Architectural Organization Research Articles (Page 1)

Rapid methodological breakthroughs over the past ten years have transformed epigenomics from bulk, population-averaged assays into single-cell, multi-omic, and intracellular spatial investigations. This review surveys the interconnected technology pillars that now map the epigenome with unprecedented breadth and resolution. First, advances in next-generation and long-read sequencing empower investigators to chart chromatin accessibility, histone and DNA modifications, and three-dimensional higher-order chromatin structure in thousands of individual cells while retaining allele-specific information across kilobase-long molecules. Second, live-cell fluorescence probes and multiplexed chromatin tracing enable visualizing the dynamic organization of epigenetic marks and genome architecture of intact nuclei and tissues. Third, integrative platforms merge base-level reads with their native 3D coordinates, providing a holistic view of gene regulation in physiologic context. We distill key biological insights yielded by each methodology, discuss unresolved and persistent limitations, and outline future directions toward routine, cost-effective investigations. Together, these innovations are redefining how we interrogate chromatin biology in health and disease.

Adaptive flexibility of cells through nonequilibrium entropy production.

Cardiac regeneration revisited: Enhanced cardiomyocyte differentiation and repair through composite microenvironments and small molecules.

Animal models, particularly the chicken embryo (CE), remain crucial for advancing developmental biology and medicine. Liver embryogenesis, a complex and tightly regulated process, is particularly susceptible to developmental abnormalities. This study presents a comprehensive 2D and 3D µCT analysis of CE liver development (HH22-HH41) using a refined 1% phosphotungstic acid (PTA) staining protocol. Our methodology yielded high-resolution visualization of liver microstructures, including intricate vascular networks, with image quality and contrast comparable to histological analysis. Quantitative assessment revealed a critical period of rapid liver growth between incubation days 6 and 9 (HH29-HH35), followed by the stabilization of hepatic vascular volume by day 10 (HH36). The ease of structural orientation in µCT datasets, enhanced by 3D renderings, further underscores the technique's utility. By establishing µCT as an effective tool for liver imaging, this study provides essential normative data on the liver's spatial organization, morphology, and vascular architecture during embryogenesis, thereby opening new avenues for research in embryology, teratology, pharmacology, and toxicology.

Animal models, particularly the chicken embryo (CE), remain crucial for advancing developmental biology and medicine. Liver embryogenesis, a complex and tightly regulated process, is particularly susceptible to developmental abnormalities. This study presents a comprehensive 2D and 3D µCT analysis of CE liver development (HH22–HH41) using a refined 1% phosphotungstic acid (PTA) staining protocol. Our methodology yielded high-resolution visualization of liver microstructures, including intricate vascular networks, with image quality and contrast comparable to histological analysis. Quantitative assessment revealed a critical period of rapid liver growth between incubation days 6 and 9 (HH29–HH35), followed by the stabilization of hepatic vascular volume by day 10 (HH36). The ease of structural orientation in µCT datasets, enhanced by 3D renderings, further underscores the technique’s utility. By establishing µCT as an effective tool for liver imaging, this study provides essential normative data on the liver’s spatial organization, morphology, and vascular architecture during embryogenesis, thereby opening new avenues for research in embryology, teratology, pharmacology, and toxicology.

Purpose This study aims to bridge the gap in leadership theories that view adaptability and opportunity recognition as separate processes. It proposes adapreneurial leadership—a mid-range theoretical construct positioned at the intersection of adaptive systems thinking and entrepreneurial execution—as a response to the strategic leadership challenges faced in digitally volatile and structurally constrained emerging marketing environments. Design/methodology/approach Grounded in dynamic capabilities, complexity leadership and effectuation theory, the study reconceptualises leadership as a systemic design capability, moving beyond conventional behaviour or relationship-based models. To empirically test this conceptualisation, and in recognition of agility’s prevalent operationalisation through quantitative constructs, the study used partial least squares-structural equation modelling (PLS-SEM), using data from 232 agile practitioners in the Indian IT sector. Findings The findings reveal that adapreneurial leadership impacts sustainable digital transformation outcomes both directly and indirectly through Agile Transformation Maturity, serving as a mediating variable. This demonstrates the model’s ability to integrate strategic adaptiveness with organisational learning frameworks, thereby enhancing agility. Research limitations/implications This study is cross-sectional and focuses on India’s IT sector. Future scholars should explore longitudinal patterns, validate scales across different geographies and assess the applicability of these scales in industries with high regulatory requirements. Practical implications This study advances the understanding of how leaders and policymakers can shift from motivational leadership to designing agile, opportunity-driven systems that embed flexibility, innovation and strategic responsiveness into organisational architecture. Social implications This study contributes to the discourse on leadership, complexity and digital transformation by establishing adapreneurial leadership as a systemic leadership capability and advancing mid-range theory with entrepreneurial intent, structural adaptability and ecosystem design. Originality/value This study introduces adapreneurial leadership as a mid-range theory, redefining leadership as the ability to design and coordinate complex systems, which enables systemic change in both constricted and turbulent contexts, and makes a key contribution to the theory. It also critiques the existing theories for their linearity and Western-centricity. It proposes an integrated model for a more relevant, empirically validated framework for digitally transforming organisations in emerging market economies.

This paper aims to develop a novel theoretical framework for organizational structures in the era of artificial intelligence (AI). It conducts a comparative analysis of traditional, modern, and postmodern organizational structures to identify limitations in accommodating AI’s autonomous capabilities. Through extensive literature review and critical analysis, the study synthesizes organizational theories with emerging AI research to propose a new paradigm integrating AI as an active participant in organizational dynamics. The findings reveal a significant theoretical gap in existing models, which predominantly treat AI as a tool rather than an autonomous agent. The proposed AI-driven paradigm emphasizes distributed intelligence, adaptive structural fluidity, human-AI symbiosis, and transparent accountability. The conceptual nature of the study calls for empirical validation across different industries and cultures. The paradigm provides a framework for managers and practitioners to redesign organizational architectures, fostering agility and ethical governance in AI-augmented environments. This research fills a critical gap in organizational theory by positioning AI as a core actor influencing structure and decision-making, offering a comprehensive model for organizations navigating the complexity of the digital age.

Meningeal lymphatic architecture and drainage dynamics surrounding the human middle meningeal artery

This article examines how organizational structures mediate the relationship between global talent networks and local economic complexity. As economies become increasingly knowledge-driven, the interaction between internationally mobile talent and local economic ecosystems has emerged as a crucial determinant of innovation capacity and economic diversification. Drawing on research from economic geography, organizational science, and talent management, this analysis identifies how organizational architecture either facilitates or impedes the translation of global knowledge flows into local economic complexity. The evidence suggests that organizations with permeable boundaries, cross-functional collaboration mechanisms, and decentralized decision-making are better positioned to leverage international talent networks to enhance local capabilities. By deliberately designing organizational structures that support knowledge transfer across geographic and cultural boundaries, firms can serve as crucial intermediaries that transform global talent mobility into locally embedded economic complexity, ultimately driving regional competitive advantage and resilience.

DNA methylation inhibitors are widely used in treating myeloid malignancies, yet their precise effects on chromatin organization and nuclear architecture remain incompletely understood. Here, the integrated molecular, cellular, and biophysical approaches to investigate the impact of azacitidine (AZA) and decitabine (DEC) on chromatin structure and nuclear mechanics in AML-007 leukemia cells are presented. Confocal microscopy revealed drug-induced alterations in nuclear morphology and actin cytoskeleton organization, with DEC inducing significant nuclear enlargement and disorganization at lower concentrations (1.0 µM) compared to AZA (5.0 µM). Chromatin condensation assays demonstrated that DEC increased chromatin accessibility in a concentration-dependent manner, while AZA produced subtler effects. Optical tweezers measurements showed both agents reduced nuclear stiffness, with DEC exerting a greater impact. Spectroscopic analysis confirmed differential drug incorporation into DNA, with higher methylation loss and structural changes observed following DEC treatment. Refractive index mapping revealed chromatin decompaction, aligning with increased accessibility and nuclear softening. These findings demonstrate that DNA hypomethylating agents exert distinct, concentration-dependent effects on nuclear organization and chromatin structure, which can be quantified through molecular and biophysical readouts. This study underscores the value of integrative methods for revealing epigenetic drug effects on chromatin architecture in leukemia cells.

The role of the cultural and educational center as a tool for the digitalization of the modern city is examined. The impact of digitalization through cultural and educational spaces on city development is considered. Key directions for the implementation of digital technologies and tools in the context of architectural treatment of cultural spaces are identified. The challenges of advanced digital technologies are considered and analyzed in their interrelation with architecture taking into account the philosophical subtext of development. Key aspects of digitalization in the global development of cultural and educational spaces are delineated. The analysis of relationships between digital language and technologies employed in cultural center spaces suggests main vectors in the development of digital environments within the framework of architectural organization of spaces.

The current relevance of the research topic is determined by the need to develop sustainable forms of agricultural activity in an urban environment, and to provide support for structural and spatial organization of ecourbanic farming centers (EUFC). The urban area is considered as a promising platform for the integration of ecologically oriented agricultural practices that contribute to restoring the natural balance, ensuring food security, and developing recreational spaces and educational initiatives in the field of sustainable agriculture. This issue is lacking theoretical and methodological foundations and models of spatial integration of the EUFC into the structure of a modern city. The purpose of the study was to identify the features of the architectural structure and spatial organization of the EUFC by matrix analysis. The study helped to identify the basic principles of eco-agrarian center location taking into account urban landscape planning characteristics; the structure of the functional zones of the EUFC; the typology of centers by functional content and their spatial architectural organization.

In multi-agent systems (MAS), autonomy is a fundamental characteristic that enables agents to operate independently and adaptively within complex environments. However, such characteristics may cause the system to fall into undesirable situations. On the one hand, purely autonomous agents are difficult to predict. On the other hand, fully controlled agents lose many of their abilities. Therefore, control frameworks have been designed in the form of organizational architectures to help address the need for balance between purely autonomous and fully controlled agents. This paper investigates the impact of organization on the autonomy of the agents. To measure this impact, we propose a set of seven metrics (Behavioral Wealth (BW), Service Wealth (SW), Frequency of Service Searches per Time (FoSST), Frequency of Service Searches per Behavior (FoSSB), Number of Service Searches (NoSS), Number of Service Demands per Behavior (NoSDB), and Number of Provided Services per Demand (NoPSD)) and apply them to a case study implemented in two configurations: with and without organizational aspects. To model organizational aspects, we adopt the Agent–Group–Role (AGR) model, chosen for its structured approach to defining agent responsibilities and interactions. The findings of this study show that the organizational aspects reduce the communication load and enhance the effectiveness of agents.

BackgroundThe Sichuan taimen (Hucho bleekeri Kimura) is a critically endangered fish species that has attracted significant attention and is classified as a national first-level protected species in China. Despite its ecological importance, genomic resources for the Sichuan taimen and its closely related species are limited, which hampers effective conservation efforts. A comprehensive understanding of the genomic characteristics of this species, particularly those related to its evolutionary adaptations and population dynamics, is essential for improving conservation strategies.ResultsWe present the first chromosome-level genome of the Sichuan taimen, with a final genome size of approximately 3.45 Gb. Notably, tandem repetitive sequences constitute 44.47% of the genome and significantly influence its genomic architecture and functional organization. Among these, VNTRs account for the majority of the tandem repeats, which Likely contributes to the relatively large genome size. Positive selection analysis revealed genes associated with immune responses and neurobiological functions, potentially facilitating adaptation to the unique environmental conditions of mountain stream habitats. Additionally, the divergence time between the Siberian taimen and the Sichuan taimen was estimated to be approximately 2.34 Mya, with a notable decline in their effective population size occurring around 1 Mya.ConclusionsThe genome assembly of the Sichuan taimen provides insights into its evolutionary adaptations, highlighting the functional significance of tandem repeats and positively selected genes. These findings establish a genomic foundation for elucidating its survival mechanisms in a unique habitat, and emphasize the critical role of integrating genomic data into conservation strategies to enhance the management of Sichuan taimen populations and contribute to the conservation of other endangered salmonid species.Supplementary InformationThe online version contains supplementary material available at 10.1186/s12864-025-12057-z.

In the digital age, the information-based transformation of business administration is an inevitable trend for enterprises to enhance management efficiency and core competitiveness. This study elucidates the connotation and significance of the information-based transformation of business administration, conducts an in-depth analysis of the challenges encountered during the transformation process, including difficulties in technology application and integration, data security and management risks, organizational and talent bottlenecks, as well as the balance between cost investment and benefits. Targeted innovative strategies are proposed, encompassing technological innovation to build an integrated information platform, data management innovation to establish a full-process governance system, organizational and talent innovation to restructure the organizational architecture and training mechanisms, and cost-benefit innovation to optimize resource allocation and evaluation. The aim is to provide effective guidance for enterprises in their information-based transformation of business administration and drive enterprises to achieve management model upgrades and sustainable development.

Organization of French level 1 adult trauma centers: A national survey.

The article investigates Piet Mondrian's Neoplasticism as a visual philosophy of modernity and analyzes its influence on the formation of a universal artistic language that remains relevant in contemporary visual design practices. The object of the study is Neoplasticism as an integral cultural project of the modern era. The subject of the study is the specificity of Mondrian's artistic language and the mechanisms of adaptation of its principles in architecture, design, and digital interfaces of the 20th–21st centuries. It is shown that Mondrian's artistic system, combining rational calculation with a utopian striving for universal harmony, formed a stable visual code. This code, based on the balance of simplest elements, proved to be an effective tool for organizing visual space far beyond painting. Special attention is paid to comparing Mondrian's approach with other directions of abstract art – Malevich's Suprematism and Kandinsky's expressive abstraction. The work applies a systemic approach, allowing to consider Neoplasticism as an integral phenomenon uniting philosophical foundations, artistic method, and socio-cultural influence. The methodology includes a comparative analysis of works by Mondrian, Malevich, and Kandinsky. The method of interpreting visual forms in a philosophical and cultural context reveals the significance of Neoplasticism's formal elements as symbols of universal categories. The scientific novelty of the research lies in the application of an integrative approach that combines art-historical analysis of the formal elements of Neoplasticism with cultural-philosophical understanding of its socio-transformational potential. Unlike existing studies focusing primarily on artistic aspects, this approach reveals the mechanisms of translation of Mondrian's visual code across different historical epochs – from the theosophical origins of the early 20th century to modern digital design practices. The results and conclusions of the study demonstrate that the principles of Neoplasticism remain relevant in contemporary visual practices, especially: in modular systems in web design (adaptive Material Design grid); in principles of visual hierarchy in UI/UX design; in spatial organization in contemporary architecture and exhibition design. The conducted analysis confirms that the cultural code of Neoplasticism continues to function as an effective tool for organizing the visual environment, offering a model for harmonious information structuring in conditions of digital overload.

Objective This study aims to develop and evaluate the wound-healing and anti-inflammatory potential of Ash–ZnO NPs and Ash–ZnO NPs gel formulation, synthesized using Saraca asoca extract and stabilized in a chitosan matrix. The goal was to overcome the limitations of conventional ZnO nanoparticles, including instability, cytotoxicity, and uncontrolled release. Significance Although ZnO nanoparticles possess antimicrobial and regenerative properties, their clinical utility is limited by aggregation and dose-dependent toxicity. The use of Saraca asoca, a medicinal plant rich in flavonoids and phenolics, provides a green synthesis approach that enhances nanoparticle stability and biological activity. Incorporation into a chitosan-based gel further improves topical application by enhancing adhesion, moisture retention, and sustained release. Chitosan also contributes additional healing benefits due to its intrinsic biocompatibility, antimicrobial activity, and role in tissue regeneration. Methods Ash–ZnO NPs were synthesized through a green co-precipitation method and formulated into a chitosan hydrogel. In vitro assays, including MTT, scratch wound, and protein denaturation tests, were used to assess cytocompatibility, fibroblast migration, and anti-inflammatory potential. In vivo wound-healing efficacy was evaluated in rats using an excision model, supported by histopathological analysis. Results The Ash–ZnO NPs gel exhibited 72.5% inhibition of protein denaturation and achieved 75.63% wound closure within 48 h. Histology confirmed organized tissue architecture and minimal inflammation. Conclusion The Ash–ZnO NPs gel represents a promising, biocompatible wound-care formulation that enhances nanoparticle stability, modulates inflammation, and accelerates tissue repair, offering strong potential for clinical dermatological applications.

In this paper I examine climate-aligned development finance initiatives (DFIs) that foster private-sector investment in ‘sustainable’ urban infrastructures. I am concerned with the ways in which they work to create the institutional preconditions for such investment. I develop the notion of Municipal Structural Adjustment (MSA) as a lens for examining the fields of intervention and modes of policy-making through which these initiatives seek to reform municipalities’ governance structures, innovate planning tools and introduce financial instruments. Empirically, this paper focuses on initiatives that get implemented in Mexican municipalities. It builds on a historical exploration of the ways in which development interventions have addressed municipal-scale institutions, as well as on expert interviews with development officers, DFI staff, municipal officials, planners and consultants to analyse these actors’ efforts to reform urban bureaucracies. I delineate three interrelated modalities of institutional adjustment that I take to characterise MSA: (1) the superimposition of DFIs’ planning schemes with government legislation and their gradual institutionalisation; (2) the burial of decisions in the development of infrastructure deals and (3) the infiltration of municipal authorities, coupled with interventions in the organisational architecture of urban bureaucracies. In contrast to earlier Structural Adjustment Programmes in which development actors enforced a clear package of reforms by holding national governments hostage to conditionality-laden policy measures, these modalities highlight how DFIs institutionalise change through subtle, vested and protracted mechanisms. To place them in the lineage of Structural Adjustment Programs indicates that MSA is a deeply political process, embedded in a political economy of structural domination.

This volume, Sedimentary Successions of the Arctic Region and their Hydrocarbon Prospectivity , developed around maps of the sedimentary successions of the Arctic Region, and contains a brief, but comprehensive compilation of geological and geophysical data characterizing all significant sedimentary successions in the Arctic, which cover 57% of the polar area north of 64° N. Its two main goals are to provide, based on present-day knowledge and data, a characterization of all Arctic sedimentary successions (or sedimentary accumulations) and to supply a snapshot of hydrocarbon-related exploration in the Arctic at the end of the first quarter of this century. To achieve these goals, we represent sedimentary successions as consisting of one or several ‘tectono-sedimentary elements’ (TSEs) based on the main tectonic regimes that formed accommodation space for accumulation of sediments. A TSE characterization template has been developed as an efficient method of organizing and presenting the most important information about the stratigraphy, structure and petroleum geology of a TSE, including the most significant exploration facts. This organizational architecture is the backbone of the volume and is a key feature that distinguishes it from other studies of Arctic sedimentary basins. The online volume includes six large-size foldout maps portraying the mapped TSEs in the Circum-Arctic context, including tectonic grain of the consolidated basement, anomalous gravity and magnetic fields, location of the Arctic sampling sites and seismic profiles.