Garbage is the main problem causing environmental problems which are dominated by organic waste. Management of organic waste must be carried out extensively, one of which is by making eco-enzymes. This study aimed to determine the production of eco-enzymes from kitchen organic waste and the characterization of eco-enzymes based on them. This type of research is an experiment with a qualitative approach. The sample used is a mixture of kitchen organic waste, namely the production and characterization of eco-enzymes which include pH, COD, BOD, TSS, and TDS. The research results obtained data on eco-enzyme production by mixing 3 components in a ratio of 1 : 3: 10 (1 part sugar, 3 parts organic kitchen waste. 10 parts water), then put into a closed container and stored in a cool, dry place, circulation air is good, and dark for 3 months. Eco-enzyme characterization showed a pH of 2.59, a TDS of 2840mg/L, a TSS of 4.46mg/L, a BOD of 60 mg/L, and a COD of 13100mg/L. Kata kunci: Produksi, Karakterisasi, Eco-enzim
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