Organic Farming Systems Research Articles

Seaweed extract and fulvic acid application affect the biomass performance, the essential oil yield and composition of Sicilian oregano grown in an organic agricultural system

Origanum vulgare ssp. hirtum is one of the taxa used as ‘oregano’ and is medicinal and aromatic plant (MAP) utilized worldwide in various sectors. Nowadays, agricultural systems are moving towards sustainable, organic, and environmentally friendly agricultural production and biostimulants have been used in recent years as a potential strategy. The objective of this research was to investigate the influence of seaweed extract based on Aschophyllum nodosum (AN) and fulvic acid (FA), applied with two different doses, on morphological parameters, fresh and dry yield, essential oil (EO) traits, total phenolic content (TPC), antioxidant activity (AA) and rosmarinic acid content (RAC) of organic oregano. Two-year experiment was conducted using a randomized complete block design with one main factor (foliar biostimulant) and 3 replicates. In AN- and FA-treated plants, increases in fresh biomass between 0.3 and 8.4 t ha−1 and in dry biomass between 0.5 and 3.0 t ha−1 were observed. The application of the lowest doses of fulvic acid (FA4) produced the highest EO contents (3.49 % and 3.46 %) and EO yields (73.3 and 97.4 kg ha−1) in both years. The application of the lowest doses of FA produced an increase (on average 4 %) in thymol content. Biostimulant application generated contrasting response, and, in some cases, a reduction in some chemical parameters compared with control plants. The findings of this study contribute to add novelty in cultivation of oregano. The application of low doses of AN and FA allows to increase yields and improve some qualities of the secondary metabolites from organic oregano.

Antioxidant, Nutritional Properties, Microbiological, and Health Safety of Juice from Organic and Conventional ‘Solaris’ Wine (Vitis vinifera L.) Farming

This study investigated the technological parameters, microbiological, and functional properties of juice from Solaris grapes grown under conventional and organic farming systems to assess how these cultivation methods influence juice quality. The one-year study focused on key aspects such as the levels of health-promoting polyphenols, the presence of mycotoxins, and pesticide residues. Organic grapes showed greater bacterial and fungal diversity, with significant differences in dominant genera. Sphingomonas and Massilia were the predominant bacteria across both systems, while Erysiphe was more common in conventional grapes, and Aureobasidium was abundant in both. Despite the presence of genes for mycotoxin production, no mycotoxins were detected in the juice or pomace. Organic juice exhibited significantly higher levels of polyphenols, leading to enhanced antioxidant properties and improved technological characteristics, including lower acidity and higher nitrogen content. However, residues of sulfur and copper, used in organic farming, were detected in the juice, while conventional juice contained synthetic pesticide residues like cyprodinil and fludioxonil. These findings highlight that while organic juice offers better quality and safety in terms of polyphenol content and antioxidant activity, it also carries risks related to residues from organic treatments, and conventional juice poses risks due to synthetic pesticide contamination.

Assessing pesticide residue occurrence and risks in the environment across Europe and Argentina

The widespread and extensive use of pesticides in European crop production to reduce losses from weeds, diseases, and insects may have serious consequences on the ecosystem and human health. This study aimed to identify 20 active substances of high health risk, based on their detection frequency within and across the environmental matrices (soil, crop, water, and sediment) and to identify their associated hazardous effects. A sampling campaign was conducted across 10 case study sites in Europe and 1 in Argentina and included conventional and organic farming systems. In 31% of cases, the detected substances were found at a higher concentration in the soil than in the corresponding crops, 93% of the compounds were fungicides, and the remainder were insecticides. 43% of the substances, 57% of which were insecticides, were detected only in soil. There was a clear relationship between soils and crops in terms of contamination, but not between water and sediment. Portuguese soil (wine grapes) had the highest number of substances (12) with average concentrations (AC) varying between 1 and 162 μg/kg, followed by French (11 substances in wine grapes) (1≤AC≤64 μg/kg) and Spanish soils (9 substances in vegetables) (3≤AC≤59 μg/kg). The crops corresponding to these soils contained a relatively high number of detected substances and several in high average concentrations (AC). The risk quotient was consistently higher for conventional farms than for organic farms. For the soils from conventional farms, 5 active substances (chlorpyrifos, glyphosate, boscalid, difenoconazole, lambda-cyhalothrin, and one metabolite: AMPA) were considered high risk. For water samples, 2 substances (dieldrin and terbuthylazine) found were high risk, and for sediment, there were 3 substances (metalaxyl-M, spiroxamine, and lambda-cyhalothrin). There were 6 substances detected in crops that are suspected to cause human health effects. Uncontaminated soil is a prerequisite for the adoption of sustainable alternatives to pesticides. Efforts are needed to elucidate the unknown effects of mixtures, including biocides and banned compounds in addition to the substances used in agriculture.

Barriers of adopting sustainable soil management practices for organic and conventional farming systems

There is a general consensus among stakeholders about the benefits of sustainable soil management practices for soil health, yet their implementation lags behind expectations. The aim of this paper is to analyse factors supporting or hindering the implementation of sustainable soil management practices in conventional and organic farming systems. This is achieved using a Logit model, based on penalized maximum likelihood estimation on survey data by 76 farmers from 10 federal states in Germany. Affected factors were categorized in two main groups: agricultural soil management practices, and barriers to their implementation. The measures for soil improving agricultural management included (I) structural landscape elements, (II) organic fertilization, (III) diversified crop rotation, (IV) permanent soil cover (V) conservation tillage, (VI) reduced weight pressure, and (VII) optimized timing of wheeling. Results show differences in preferences between conventional and organic farmers for key sustainable soil management practices, including conservation tillage, optimized wheeling timing, reduced weight pressure, and diversified crop rotation. Economic constraints decrease crop rotation diversification in conventional systems by 14%. Conservation tillage raises the chance of a farm being conventional by 16%, while optimizing wheeling timing reduces it by 60%, highlighting soil compaction concerns among organic farmers. Marginal effects confirm that economic factors, not knowledge, are the main barriers to sustainable soil management. Inconsistent policies, lack of support, and insufficient financial incentives from government institutions can hinder the willingness of farmers to adopt these practices, further exacerbating the barriers to adoption.

Activity of Common Thyme (Thymus vulgaris L.), Greek Oregano (Origanum vulgare L. ssp. hirtum), and Common Oregano (Origanum vulgare L. ssp. vulgare) Essential Oils against Selected Phytopathogens

The aim of this study was to determine the activity of common thyme (Thymus vulgare L.), Greek oregano (Origanum vulgare L. ssp. hirtum), and common oregano (Origanum vulgare L. ssp. vulgare) essential oils (EOs) against selected phytopathogenic microorganisms in relation to their chemical profile. The EOs were obtained from the herbs of 2-year-old plants cultivated in the organic farming system in a temperate climate in Central Europe. The EOs’ composition was determined by GC/MS and GC/FID. The investigated species were represented by the following three chemotypes: ‘thymol’ for common thyme, ‘carvacrol’ for Greek oregano, and mixed ‘caryophyllene oxide + β-caryophyllene’ for common oregano. The antimicrobial activity of the EOs was assessed based on minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) values. The plant pathogenic bacteria Pseudomonas syringae, Xanthomonas hortorum, Erwinia carotovora, and fungi: Fusarium culmorum, Alternaria alternata, Botrytis cinerea, Epicoccum purpurascens, Cladosporium cladosporioides, Phoma strasseri, and Pythium debaryanum were tested. The EOs revealed a stronger inhibitory effect against fungal growth in comparison to bacterial growth (MIC: 0.016–2 µL/mL for fungi and 0.125–4 µL/mL for bacteria). Common thyme and Greek oregano EOs indicated stronger antimicrobial power than common oregano EO. These results were associated with the chemical profile of the analysed EOs. The growth of examined bacteria and fungi strains (in particular, X. hortorum, F. culmorum, and P. debaryanum) were negatively correlated with the content of phenolic monoterpenes and monoterpene hydrocarbons. Among the tested strains, P. strasseri turned out to be the most sensitive (MIC 0.016 µL/mL) and E. carotovora the most resistant (MIC 0.250–4 µL/mL) to all investigated EOs.

Integrated Management of Sclerotinia Stem Rot of Soybean Including Organically-Allowed Fungicides in the Midwest United States

Sclerotinia stem rot (SSR) is an economically important disease of soybean, especially in the Great Lakes region of the United States. Few studies on SSR have considered organic management practices and how integrated management techniques best apply in these systems. Trials in Wisconsin and Indiana aimed to evaluate management techniques available to organic farmers for their efficacy in SSR control while preserving yield. The practices evaluated included genetically resistant cultivars, tillage techniques, and the application of Organic Materials Review Institute (OMRI) listed foliar fungicides. The resistant cultivar had significantly lower SSR than the susceptible cultivar in three site-years (P < 0.01). The resistant cultivar also had significantly greater yield than the susceptible cultivar in two site-years (P < 0.05). Using a roller-crimped rye cover crop resulted in mixed results on SSR and yield. No effect on SSR or yield by OMRI listed foliar fungicides was observed (P > 0.05). Overall, the results emphasize the importance of planting a resistant soybean cultivar to manage SSR in organic farming systems in the Midwestern United States.

Integrated Chemometrics and Data-Independent Acquisition Proteomics for the Discovery of Meat Authenticity Biomarkers: A Study on Early Post-Mortem Pectoralis major Muscle Proteomes of Ross 308 and Ranger Classic Chicken Produced by Organic versus Antibiotic-Free Farming Systems.

Many factors, such as the farming systems and preslaughter rearing practices, can influence the physiological and metabolic functions of poultry with consequent effects on poultry meat quality. In this trial, label-free shotgun proteomics was used to analyze the early post-mortem Pectoralis major muscle proteomes of Ross 308 and Ranger Classic chicken strains raised under two divergent farming systems these being organic and antibiotic-free. The combination of chemometrics using partial-least-square discriminant analysis (PLS-DA) and shotgun proteomics allowed clear discrimination between the different groups. Chicken strains were discriminated by differences in the abundance of 73 and 62 proteins within the antibiotic-free and organic farming systems, respectively. The abundances of 71 and 52 proteins were impacted by the farming system within the Ross 308 and Ranger Classic chicken strains, respectively. The analyses allowed for the proposal of several putative biomarkers of meat authenticity, which were found to be related to muscle structure and energy metabolism pathways. This study is a significant step forward in elucidating the potential of proteomics profiling and chemometrics in chicken meat, which may provide opportunities for the efficient assessment of chicken authenticity.

Conventional agriculture affects sex communication and impacts local population size in a wild bee

Man-made agricultural stressors have been identified to compromise the reproductive dynamics of bee populations within agricultural environments. With the aid of bee hotels, we explored the influence of conventional and organic farming systems on local population size and body traits of the mason bee, Osmia bicornis, in southern Germany. We further used a chemical ecology approach and bioassays to test whether farming management influence male pre-copulatory behaviors. We observed a positive relationship between the extent of organic agriculture in the landscape and both overall brood cell production and nesting frequency. Moreover, farming systems were found to influence body traits, with bees from organic sites being smaller in size and having a different cuticular hydrocarbon composition compared with those at conventional sites. Bioassays revealed that males were more sexually attracted to freeze-killed females from conventional sites compared with those from organic sites. Intriguingly, treating females from organic fields with synthetic semiochemicals enhanced their sexual attraction to levels comparable with females from conventional sites. Our findings shed light on the intricate interplay between farming practices and the reproductive behaviors of wild mason bees, emphasizing the need for a comprehensive understanding of these dynamics for effective conservation and management strategies.

Impact of natural and organic farming systems on growth, yield and economics of greengram

Impact of natural and organic farming systems on growth, yield and economics of greengram

Improved Tomato Breeding Lines Adapted to Organic Farming Systems Have Enhanced Flavor, Yield, and Disease Resistance

Most of the tomato (Solanum lycopersicum) varieties currently used in organic farming were bred for conventional farming, often characterized by high-input use. These varieties do not perform as well in low-input organic systems, generating the need to develop varieties that are adapted to organic management systems. This project focused on improving flavor, disease resistance, and yield, all identified as key traits by organic tomato farmers in the Upper Midwest, USA. Ten advanced tomato breeding lines and two check varieties were developed and evaluated for 16 traits in organic high tunnel systems in 2020 and 2021. The line CSDE-F6.47 averaged 6.32 kg/plant and obtained high flavor intensity and overall flavor scores (3.78 and 3.69 out of 5, respectively). The line JBDE-F5.31 was another outstanding line, with a yield of 5.18 kg/plant, with good flavor intensity (3.32) and overall flavor (2.92) scores. Broad-sense heritability of marketable weight per plant was high (0.91), and the genetic variance was also high, which shows the opportunity to continue to increase the marketable weight in lines with excellent flavor. A significant positive correlation was found between overall flavor and °Brix (0.56), and titratable acidity (0.70), indicating that both measurements can be good predictors of overall flavor. The most promising lines will be further evaluated on-farm to evaluate their potential as releasable varieties.

Crop rotation diversity and tillage effects on soil and wheat grain nutrient concentration in an organically-managed system

Consumer demand for high quality organic produce in Canada has in recent years contributed to the development of a more sustainable organic agriculture system that increased productivity without decreasing soil fertility. The objective of this 4-yr field study was to determine if an organic system with diversified crop rotations under reduced tillage would produce high quality spring wheat (Triticum aestivum L.) grain and improve soil fertility. A simplified rotation (SR) consisting of a forage pea (Pisum sativum L.) green manure (GM)-spring wheat sequence and a diversified rotation (DR, forage pea GM-oilseed-pulse crop-spring wheat sequence) were compared under two tillage intensities (high tillage [HT] and low tillage [LT]). The oilseed alternated between mustard (Sinapis alba L.) and flax (Linum usitatissimum L.), and the pulse alternated between field pea and lentil (Lens culinaris L.). Generally, nutrient concentrations in wheat grain were mostly significantly higher under LT than HT and in the DR compared with the SR. In addition, tillage-rotation system influenced soil zinc, calcium, magnesium, and sodium concentrations. However, grain Cd concentration was higher in the LT treatment under the DR sequence relative to other treatments. Our results indicate that organically managed diversified crop rotations under reduced tillage can produce wheat grains with high nutritional value for humans and livestock but may not increase soil micronutrient concentrations.

The Role of Organic Farming for Climate Change Mitigation and Sustainable Development: A Review

Climate Change, exacerbated by human activities such as fossil fuel combustion and greenhouse gas emissions like carbon dioxide (CO2), is a pressing global crisis that disrupts long-term weather patterns, ecosystems, and human well-being. Organic agriculture offers a viable solution to mitigate these effects. By lowering greenhouse gas emissions and enhancing soil carbon sequestration, organic farming strengthens ecosystem resilience. Practices such as eliminating synthetic fertilizers and pesticides, rotating crops, and utilizing organic waste foster robust agricultural systems capable of enduring extreme weather and water scarcity. This paper provides an in-depth analysis of how organic farming contributes to climate change mitigation, detailing practices that support soil carbon sequestration, reduce nitrogen oxide (N2O) emissions, and boost biodiversity. It also addresses challenges such as emissions measurement and consumer behaviour, emphasizing the need for further research and supportive policies. Organic agriculture not only advances sustainable development but also promotes food security and environmental sustainability in the face of climate change.

Enhanced Soil Fertility and Carbon Dynamics in Organic Farming Systems: The Role of Arbuscular Mycorrhizal Fungal Abundance.

Arbuscular mycorrhizal fungi (AMF) are critical for soil ecosystem services as they enhance plant growth and soil quality via nutrient cycling and carbon storage. Considering the growing emphasis on sustainable agricultural practices, this study investigated the effects of conventional and organic farming practices on AMF diversity, abundance, and ecological functions in maize, pepper, and potato-cultivated soils. Using next-generation sequencing and quantitative PCR, we assessed AMF diversity and abundance in addition to soil health indicators such as phosphorus content, total nitrogen, and soil organic carbon. Our findings revealed that, while no significant differences in soil physicochemical parameters or AMF diversity were observed across farming systems when all crop data were combined, organic farming significantly enhances AMF abundance and fosters beneficial microbial ecosystems. These ecosystems play vital roles in nutrient cycling and carbon storage, underscoring the importance of organic practices in promoting robust AMF communities that support ecosystem services. This study not only deepens our understanding of AMF's ecological roles but also highlights the potential of organic farming to leverage these benefits for improving sustainability in agricultural practices.

Eco-Friendly Cow's Milk Production in Organic and Biological Farming Systems

Eco-Friendly Cow's Milk Production in Organic and Biological Farming Systems

Crop Nutrition and Soil Fertility Management in Organic Potato Production Systems

Organic farming is a comprehensive production management system that fosters and improves the health of agroecosystems, encompassing biodiversity, biological cycles, and soil biological activity. The potato (Solanum tuberosum L.) is a crucial crop in organic farming systems, standing out as one of the most highly demanded organic products on the market. Among all crops, with potatoes, there is a very large yield gap between organic and conventional systems, attributable mainly to its intensive nutrient demands. The present review, considering the most relevant scientific literature worldwide, discusses the contemporary state of knowledge on crop nutrition and soil fertility management in organic potato crop production, analyzing the effects of animal manures, green manures, organic amendments, and biostimulants on organic potato tuber yield and quality. Overall, the main findings show a particular combination is needed to effectively maintain good soil fertility, satisfy the nutritional needs of the crop, and overcome the difference in potato yield between organic and conventional farming methods while meeting consumer demand. This combination entails using an animal manure or leguminous green manure with an organic soil amendment, and even better with a biofertilizer, such as a mycorrhizae-fungus-based one. It also emerged that more targeted studies are needed to select appropriate cultivars for organic potato farming systems to optimize this environmentally friendly production method.

Characterization of differences in seed endophytic microbiome in conventional and organic rice by amplicon-based sequencing and culturing methods.

In this paper, we studied the differences in the endophytic microbial composition of rice seeds grown in conventional and organic farming systems. Our results demonstrate a greater bacterial diversity in conventional farming, while organic farming showcases a higher fungal diversity. Additionally, our research reveals the ability of seed bacterial endophytes to inhibit the growth of three fungal pathogens responsible for causing seedling blight in rice. This study provides valuable insights into the potential use of beneficial seed microbial endophytes for developing a novel microbiome-based strategy in the management of rice diseases. Such an approach has the potential to enhance overall plant health and improve crop productivity.

Impact of mechanical weed control on soil N dynamics, soil moisture, and crop yield in an organic cropping sequence

Mechanical weed control is a major element of weed suppression in organic farming systems. In addition to the direct effect on weed growth, mechanical weeding, such as harrowing or hoeing, is known to induce side effects on several soil- and crop-related properties. In this study, we investigated the impact of mechanical weeding on soil mineral nitrogen (SMN), soil moisture, and crop yield in an organic crop rotation of grass-clover (Lolium multiflorumLam., Trifolium pratense L.), silage maize (Zea mays L.) and winter barley (Hordeum vulgare L.). The experiment was conducted in two consecutive years (2021, 2022), where each crop was grown in each year on a Plaggic Anthrosol with sandy loam in North-West Germany. Two weed control treatments (mechanical: harrowing, hoeing; chemical: herbicide application) were implemented in a randomized block design with four replications. Greater net nitrogen (N) mineralization in maize compared to winter barley were attributed to the incorporation of grass-clover residues before sowing of maize and greater mineralization potential during the maize growing season. Higher weed growth in maize after mechanical weeding resulted in a reduction of up to 47% in SMN content in the topsoil. In barley, no differences in weed suppression were observed between the treatments and only small effects on SMN were determined after mechanical weeding. The soil water content in the mechanically weeded plots was significantly higher at several events in both years and for both crops, which was attributed to increased water infiltration by disrupting the soil crust. Neither crop yield nor N uptake in harvest products was affected by the different treatments.

Performance and Sustainability of Organic and Conventional Cotton Farming Systems in Egypt: An Environmental and Energy Assessment

Cotton cultivation is resource-intensive, posing significant environmental challenges, especially with conventional farming methods. Growing interest in sustainable agriculture drives the exploration of organic farming as a potential alternative with lower environmental impacts. Despite its benefits, organic farming often faces criticism for lower crop yields, sparking debates on the trade-offs between productivity and environmental impact. This study hypothesizes that organic cotton farming will have a smaller environmental footprint and higher energy efficiency compared to conventional methods. To test this hypothesis, a cradle-to-farm gate energy analysis and life cycle assessment (LCA) were conducted on both organic and conventional seed cotton production systems in the Beheira governorate of Egypt. The ReCiPe 2016 midpoint and endpoint characterization model was used for an environmental impact assessment. The impacts were evaluated using two functional units: one ton of seed cotton and one hectare of cultivated cotton. The findings revealed that organic cotton outperforms conventional cotton in net energy gain, efficiency, and profitability, with higher productivity and lower energy intensity. Regardless of the functional unit used (mass- or land-based), the assessed organic systems generally show a better environmental performance than the conventional systems in the local context, even when accounting for data uncertainty. This is due to lower input intensity and the use of less energy-intensive organic fertilizers and bio-fertilizers. Fertilization and irrigation are key factors influencing environmental impacts, with fertilization affecting midpoint impacts and irrigation affecting endpoint impacts. Therefore, precision fertilization, efficient irrigation practices, and effective nutrient and soil moisture management are recommended to minimize environmental impacts. Subsequent studies could explore whether similar patterns are observed in different geographic regions and evaluate additional social and economic aspects of cotton sustainability beyond environmental impacts. Future agricultural LCAs should use both mass-based and area-based functional units to capture a broader range of environmental effects and evaluate the co-benefits and trade-offs between organic and conventional practices.

Effects of several long-term soil health treatments on populations of Pratylenchus penetrans and the soil microbial community

Soil suppressiveness can reduce the damage by plant parasitic nematodes (PPN) in agricultural soils and is conveyed by the activity of soil microorganisms. While natural suppressiveness has been reported, it is still poorly understood if soil suppressiveness can be elicited by manipulating the soil microbial community. In the present study we assessed the number of the Pratylenchus penetrans (Pp) and the bacterial and fungal community composition over 7 years in a long-term soil health experiment. The field experiment consisted of an organic and conventional agricultural land management system and three soil health treatments (SHT): an untreated control (CT), anaerobic disinfestation (AD) and a combination of marigold cover cropping, compost and chitin amendment (CB). The land management systems were kept continuously, while the soil health treatments were applied only twice in seven years. The microbial community significantly differed between the organic and conventional system, but there was no significant difference in Pp numbers between the two systems. However, both the CB treatment and to a lesser extent the AD treatment reduced Pp numbers and increased yield with the effect being the strongest in the years immediately after the treatment. Accordingly, both the bacterial and fungal community differed significantly between the treatments, the differences being largest in the years after the treatments. Notably, the CB treatment elicited both long-term changes in the microbial community and a reduction of Pp numbers lasting for at least three years. These results indicated that a combination of treatments can lead to an altered soil microbial community in combination with persisting suppressiveness of Pp.

Energy Consumption and Sustainability Analysis in Organic Farming Systems on Arabica Coffee in Rural Farmer Latimojong Mountain in South Sulawesi, Indonesia

Energy Consumption and Sustainability Analysis in Organic Farming Systems on Arabica Coffee in Rural Farmer Latimojong Mountain in South Sulawesi, Indonesia