Oral Soft Tissue Research Articles

MarginView3D: A Novel Software Enabling Comprehensive Three-Dimensional Surgical Pathology Documentation for Improved Intra- and Postoperative Cancer Care.

Surgical pathology reports are crucial to the delivery of personalized and coordinated cancer management. However, traditional reports often lack clarity and comprehensiveness, which can lead to misinterpretation or suboptimal therapeutic planning. To address these issues, we developed MarginView3DTM (MV3D), a novel surgical pathology reporting software. This software integrates real-time three-dimensional (3D) visualizations of surgical specimens, surgical defects, anatomic models, annotated radiographs, and audiovisual narrative summaries from surgeons and pathologists to enhance the care of patients with head and neck cancer. We detail the internally developed MV3D surgical pathology reporting platform created by a multidisciplinary team incorporating open-source platforms, commercial 3D scanners, and medical illustrators. This software was piloted in 26 head and neck cancer cases over 6 months. The software facilitates comprehensive margin mapping and pathology reporting in a range of procedures: palatomaxillectomy (n=5), parotidectomy (n=5), mandibulectomy (n=5), oral cavity soft tissue resection (n=5), laryngectomy/laryngopharyngectomy (n=4), thyroidectomy (n=1), and facial cutaneous malignancy resection (n=1). Notably, 24 out of 26 surgeries achieved well-documented negative final margin statuses. The median number of inadequate margins ("margins at-risk") per case was 1.5 (range 0-6). The median number of supplemental tissue samples harvested per case was two (range 0-13). MV3D enhances surgical pathology documentation by consolidating precise anatomic orientation, clear margin reconciliation, and narrative summaries offered by the surgeon and pathologist into one platform to aid in the treatment of head and neck cancer. MV3D has the potential to improve margin reporting, care coordination, adjuvant treatment planning, and personalized cancer management.

Presenting a novel model to visualize the interaction process between blue (445 nm) and infrared laser radiation (810 nm) in oral soft tissue.

Presenting a novel model to visualize the interaction process between blue (445 nm) and infrared laser radiation (810 nm) in oral soft tissue.

Innovative applications of electrocautery in oral soft tissue lesion treatment: Focus on mucoceles

Introduction: Oral mucoceles are common benign lesions in children, frequently caused by trauma to minor salivary glands, with the lower lip being the most affected site. Chronic lip biting is a common etiological factor. While conventional excision is widely practiced, electrocautery presents a minimally invasive alternative with superior hemostasis and healing properties, making it suitable for pediatric patients. Case History: A 10-year-old male presented with a painless, clear, soft swelling on the left side of the lower lip, present for approximately one month. The lesion measured 5 mm in diameter. The patient reported a habitual lip biting behavior. Based on clinical findings, an extravasation-type mucocele was diagnosed and the lesion diagnosis was confirmed by histopathology anatomy (HPA). The lesion was excised under local anesthesia using electrocautery, along with adjacent minor salivary glands to prevent recurrence. The procedure was bloodless, quick, and required no sutures. Postoperative recovery was uneventful, with observed within one weeks follow up. Discussion: Electrocautery offers precise tissue removal, excellent intraoperative control, and minimal postoperative morbidity. Compared to scalpel or laser excision, it is cost-effective and better tolerated by pediatric patients. Addressing etiological habits, such as lip biting, is essential to prevent recurrence. Conclusion: Electrocautery is an effective, minimally invasive method for managing soft tissue lesion, specifically a mucocele in a pediatric patient. Its ability as an innovative treatment that results in minor bleeding and promotes accelerating recovery.

Preparation and Characterization of PVA/PVP/CS Bionic Hydrogels.

Poly(vinyl alcohol) (PVA) hydrogels are water-rich biomimetics with soft properties. However, the mechanical properties and surface properties of PVA hydrogels are quite different from natural oral soft tissues when applied to a simulated oral environment. To select suitable enhancement to modify PVA hydrogel for simulating oral soft tissue, an ingenious strategy is developed for enhancing PVA hydrogel in this study. The PVA/polyvinylpyrrolidone (PVP) (APV) hydrogel was prepared by solvent exchange and then cross-linked with chitosan (CS) to prepare a PVA/PVP/CS (PPC) composite hydrogel, and their surface, mechanical, lubrication properties, and biocompatibility were deeply investigated. The results demonstrate that the solvent exchanging system using DMSO promoted the cross-linking density of PVA and PVP. When the mass ratio of PVA/PVP was 3:1, the APV hydrogel had the excellent tensile property. After the cross-linking between APV and CS, the compression modulus of PPC hydrogels was 212% higher than that of PVA hydrogels and was closer to that of natural human tongue. The superhydrophilicity of APV was significantly adjusted by CS, and the water contact angle of PPC2 reached 27.07°. The lubrication properties of PPC were greatly improved compared with PVA and APV, and the average friction coefficient of the PPC2 hydrogel decreased by 62% compared with the APV hydrogel and could maintain stable lubrication properties under a simulating oral environment. The bionic PPC hydrogels had better biocompatibility than APV, and the survival rate of MC3T3-E1 cultured in PPC extract for 48 h reached 96.18%. This preparation strategy has greatly improved the comprehensive properties of PVA hydrogels without affecting their biocompatibility, which provides valuable insights into the enhancement of PVA-based hydrogels and the preparation of oral substitute bionic materials.

Oral Soft Tissue and Jawbone Sarcomas: A Retrospective Clinicopathologic Analysis of 128 Cases from Two Institutions and Comprehensive Literature Review

PurposeOral soft tissue and jawbone sarcomas (OSTJS) are rare neoplasms accounting for only 1% of all intraoral malignancies. As a result, robust epidemiologic data pertaining to OSTJS are limited. Here, we present a collaborative, retrospective analysis of the clinicopathologic characteristics of 128 cases of OSTJS, together with a comprehensive review of the literature.MethodsArchived OSTJS cases (2000–2022) were retrieved from the electronic laboratory databases of the oral pathology services at The Ohio State University and University of Minnesota. Patient age and sex, anatomic site and histopathologic diagnosis were recorded.ResultsAmong 128 OSTJS, 123 (96.1%) were primary and 5 (3.9%) metastatic (M: F = 1.5:1; mean age = 43.7 years, range = 4-102 years). Most OSTJS presented in adults (113, 88.3%; mean age = 47.8 years) with only 15 pediatric cases (11.7%; mean age = 13 years). Favored sites included the mandible (48, 37.5%), maxilla (39, 30.4%), gingiva (15, 11.7%), palate (13, 10.2%), and tongue (4, 3.1%). In adults, osteosarcoma represented the predominant OSTJS (58, 51.3%), followed by Kaposi sarcoma (18, 15.9%), leiomyosarcoma (7, 6.2%), chondrosarcoma (6, 5.3%), low-grade myofibroblastic sarcoma (5, 4.4%), and 4 each (3.5%) of angiosarcoma, rhabdomyosarcoma and undifferentiated pleomorphic sarcoma. Similarly, osteosarcoma comprised the most common OSTJS histotype in the pediatric population (8, 53.3%), followed by Ewing sarcoma (4, 26.7%) and 1 each (6.7%) of TFCP2::EWSR1-rearranged rhabdomyosarcoma, mesenchymal chondrosarcoma, and alveolar soft part sarcoma.ConclusionOSTJS represent an uncommon, histopathologically diverse, subset of mesenchymal malignancies. In our series, most patients were adults in their 4th − 5th decade with a broad age range and a slight male predilection. Overall, jawbone osteosarcoma and Kaposi sarcoma accounted for two-thirds of OSTJS cases in this cohort. While the diagnosis of OSTJS relies heavily on routine light microscopic findings, ancillary immunohistochemistry and/or cytogenetic studies are frequently warranted.

Esthetic and Healing Outcomes of Oral Fibroma Excision Using a Diode Laser: A Case Report

This case report evaluates the clinical outcomes of oral fibroma excision using a 980 nm diode laser, focusing on its effectiveness in reducing postoperative discomfort, promoting healing, and improving esthetic outcomes. A 980 nm diode laser was used in CW mode at 2 watts of power to remove two fibrous masses on the buccal mucosa from a 65-year-old male patient who had presented with these lesions. The procedure was performed under local anesthesia using 2% lidocaine with 1:80,000 epinephrine. Postoperative evaluations were performed at follow-up visits on the 3rd day, 1st week, 2nd week, and 4th week to evaluate bleeding, pain, edema, functional interference, mucosal scarring, and healing through standardized clinical indices. At each follow-up visit, photographic documentation was collected to objectively track the healing progress. The procedure resulted in no bleeding, no postoperative swelling, and mild pain that did not necessitate analgesics. The recovery of function was swift. Esthetic evaluation revealed no scarring on the left side and a minor scar on the right side, with excellent overall appearance. The left fibroma site underwent complete healing by the second week, whereas the right site necessitated four weeks for full reepithelialization. The results showed that the 980 nm diode laser is a suitable substitute for traditional scalpels for oral soft tissue surgery, with few complications, mild pain after the procedure, and fast healing time. This case highlights the clinical advantages of diode lasers in minor oral surgical procedures and supports their broader adoption in clinical practice.

Histological Evaluation of Oral Soft Tissue Biopsy by Dual-Wavelength Diode Laser: An Ex Vivo Study

Background: Diode lasers are valuable in oral surgery due to their excellent hemostasis, minimum post-operative pain, and minimally invasive procedures. A dual-wavelength diode laser in dentistry combines two distinct wavelengths, typically 450 nm and 808 nm, to provide a versatile approach to soft tissue procedures. This ex vivo study investigated the quantity of thermal effects of a dual-wavelength diode laser on porcine lingual mucosa to determine the optimal laser parameters for oral soft tissue biopsies and to improve the reliability of histological evaluation. The presence of thermal damage in the prelesional margins may compromise the diagnostic accuracy, particularly in cases of suspected malignancy. Methods: Thirty-six porcine lingual mucosa samples were excised using a diode laser (Wiser 3, Doctor Smile) in continuous wave (CW) and pulsed wave (PW) modes at average powers of 2, 3, and 4 W. The samples, preserved in 5% buffered formalin, underwent histological evaluation to measure epithelial and connective tissue damage. Results: The study demonstrated variable thermal effects depending on the laser mode and power settings. Minimal epithelial damage (0.62 mm) was observed at 2 W CW, while maximum damage (3.12 mm) occurred at 4 W pulsed wave (PW). Connective tissue exhibited slightly greater damage than epithelial tissue, with minimal damage (0.53 mm) at 4 W CW and maximum damage (3.19 mm) at 4 W pulsed wave (PW). Statistical analyses were performed using t-tests and ANOVA and revealed significant differences in tissue damage between certain groups, highlighting the impact of laser parameters on thermal effects. Conclusions: The dual-wavelength diode laser seems to have good surgical properties and is suitable for managing complex clinical cases. Although the low power average showed minimal thermal damage, for the importance of the diagnosis of suspected lesions of malignancy, a 2 mm prelesional margin should be maintained.

Exploring the non-toxic therapeutic potential of dioscorea communis in combating oral pathogenic bacteria and their effect on hard and soft oral tissues

Exploring the non-toxic therapeutic potential of dioscorea communis in combating oral pathogenic bacteria and their effect on hard and soft oral tissues

Systemic Ozone Therapy Improves Oral Hard and Soft Tissue Healing in Medication-Related Osteonecrosis of the Jaw (MRONJ): A Study in Senescent Female Rats.

Background/Objectives: Medication-related osteonecrosis of the jaw (MRONJ) is a challenging condition often associated with bisphosphonate use, leading to impaired bone healing and difficult clinical management. Given the lack of predictable therapeutic options, this study investigated the effects of systemic ozone therapy on MRONJ healing. This study aimed to analyze the effects of systemic ozone therapy on oral hard and soft tissue healing in senescent rats with medication-related osteonecrosis of the jaw (MRONJ) induced by antiresorptive therapy. Methods: Twenty-eight senescent Wistar rats, aged eighteen months and weighing ~350 g, were used for this study. The animals were divided into four groups. The negative control (SAL) group received saline applications, while the control-treated (SAL+OZ) group received saline applications and ozone therapy (0.7 mg/kg). The MRONJ (ZOL) group received Zoledronate, an intravenous antiresorptive drug (100 μg/kg), and the MRONJ-treated (ZOL+OZ) group received zoledronate application and was treated with systemic ozone therapy (0.7 mg/kg). All rats underwent molar extraction in the third week of the experiment and were euthanized in the seventh week of the experiment. The mandibles were resected, reduced, and prepared for microtomographic analysis, histopathological/histometric analysis, and immunohistochemistry. Results: The ZOL group presented characteristics of vitreous, non-vital, and dense bone, poor vascularization, and high values of inflammation markers compatible with MRONJ. In contrast, the ZOL+OZ group exhibited improvement in alveolar bone and soft tissue healing, a decrease in nonvital bone area, and modulation of local inflammation. Conclusions: It can be concluded that Ozone therapy improved oral hard and soft tissue healing of MRONJ in senescent female rats subjected to antiresorptive drugs and might be considered for future clinical applications.

Clinical efficacy of composite transplantation of fascia lata-anterolateral thigh flap in repairing complex defects after radical tumor resection in maxillofacial region

Objective: To investigate the clinical efficacy of composite transplantation of fascia lata-anterolateral thigh flap for repairing complex defects after radical tumor resection in maxillofacial region. Methods: This study was a retrospective observational study. From January 2023 to January 2024, 12 patients (7 males and 5 females, aged 42 to 60 years) meeting the inclusion criteria with complex defects after radical tumor resection in maxillofacial region were treated at the Department of Burns and Cutaneous Surgery of the First Affiliated Hospital of Air Force Medical University. After radical tumor resection, the sizes were 6.0 cm×4.0 cm to 11.0 cm×10.0 cm for skin and soft tissue defects and 4.0 cm×3.0 cm to 11.0 cm×5.0 cm for buccal mucosa-zygomatic arch defects. The anterolateral thigh flap with area ranging from 7.0 cm×5.0 cm to 12.0 cm×11.0 cm was first designed and harvested, followed by the underlying fascia lata (ranging from 4.0 cm×3.0 cm to 11.0 cm×5.0 cm). Then, the fascia lata was used first to reconstruct the oral lining, and the anterolateral thigh flap was subsequently employed to repair the residual skin and soft tissue defects. Postoperatively, the survival of the patient's flap was observed, the epithelialization time of the fascia lata used as lining was recorded, and wound healing at the donor and recipient sites was monitored, along with whether complications such as vascular crisis, oral fistula, or infection were present at the recipient site. During follow-up, the epithelialization of the fascia lata as a lining was observed, the mouth opening degree of the patient was measured, the occlusal function was evaluated, and the facial appearance and scar condition in donor site were observed. Results: Postoperatively, all flaps of patients survived successfully, with the fascia lata used as a lining achieving complete epithelialization within 8-10 days. Only one patient with maxillary sinus squamous cell carcinoma experienced delayed wound healing at the flap margin, which was resolved after dressing changes; the recipient site wounds in the remaining patients healed well. All the donor site healed well. No vascular crisis, oral fistula, infection, or other complications occurred at the recipient sites. During 3 months of follow-up, the oral mucosa had covered the surface of the fascia lata; the vertical mouth opening was 3 transverse fingers in 9 cases, 2.5 transverse fingers in 1 case, 2 transverse fingers in 1 case, and 1.5 transverse fingers in 1 case, the horizontal mouth opening was 3-5 cm; chewing and swallowing functions were normal. All patients exhibited facial symmetry with the surgical area being full, and only linear scars were left in the donor sites. Conclusions: The composite transplantation of fascia lata-anterolateral thigh flap demonstrates excellent efficacy in repairing complex defects after radical tumor resection in maxillofacial area. Through staged reconstruction of both the oral barrier and soft tissue defects, this approach significantly reduces the risk of postoperative complications while achieving functional recovery and aesthetic reconstruction.

Advising patients with dental and oral soft tissue pain Free CPD for dentists and other dental health professionals.

Advising patients with dental and oral soft tissue pain Free CPD for dentists and other dental health professionals.

3D Bioprinting and Artificial Intelligence‐Assisted Biofabrication of Personalized Oral Soft Tissue Constructs (Adv. Healthcare Mater. 13/2025)

3D Bioprinting and Artificial Intelligence‐Assisted Biofabrication of Personalized Oral Soft Tissue Constructs (Adv. Healthcare Mater. 13/2025)

Optimizing Oral Soft Tissue Healing: A Comparative Evaluation of 450 nm and 976 nm Diode Lasers with Adjunctive Hyaluronic Acid and Low-Level Laser Therapy. Histological & immunohistochemical study

Optimizing Oral Soft Tissue Healing: A Comparative Evaluation of 450 nm and 976 nm Diode Lasers with Adjunctive Hyaluronic Acid and Low-Level Laser Therapy. Histological & immunohistochemical study

Mouth protector as a preventive measure against unintended oral mucosal injury during the use of dental instruments

Background: Modern dental practice frequently involves the use of invasive instruments. In complex clinical settings with limited access to the operative field, such tools can cause unintended trauma to the oral mucosa. Existing protective methods have notable limitations and do not fully prevent mechanical injury to oral soft tissues. As a potential solution, the authors propose the use of mouth protectors. Aim: To evaluate the clinical efficacy of the proposed mouth protectors. Methods: A single-center, observational, prospective study was conducted involving 40 patients aged 20 to 60 years undergoing prosthodontic treatment with fixed dental prostheses. Participants were randomly assigned to two equal groups. Group A received treatment using the proposed mouth protectors during tooth preparation. Group B underwent tooth preparation without mouth protectors. The total duration of the study was 5 weeks. Throughout the study, all cases of unintended oral mucosal injury occurring during the tooth preparation stage were recorded for both groups using visual inspection and staining, and documented in a summary table. The surface area of the lesions was measured photometrically, and patient-reported symptoms were assessed via structured interviews. The results were compared between groups to assess the effectiveness of the mouth protectors. Results: All participants completed the study. The incidence of oral mucosal injuries in Group A was 76.9% lower than in Group B. The total lesion area in Group A was 74.6% smaller than in Group B. Conclusion: The findings demonstrate the effectiveness of the proposed mouth protectors in reducing mechanical injury to the oral mucosa during prosthodontic procedures.

Extranasopharyngeal Angiofibroma in the Tongue: A Case Report With Literature Review.

Angiofibroma is a rare, benign vascular tumor traditionally associated with the nasopharynx of adolescent males. In contrast, extranasopharyngeal angiofibromas (ENAs) exhibit a wider demographic distribution and may arise in diverse anatomical sites. We report a case involving a 45 year-old woman presenting with a 4 month history of a mass located on the lateral border of the anterior tongue. Complete excision under local anesthesia was performed. Histopathological analysis demonstrated a proliferation of irregular vascular channels lined by benign endothelial cells, set within a fibrovascular stroma, findings consistent with angiofibroma, and devoid of malignant features. Given that lingual involvement is exceptionally rare, this represents only the second documented case of tongue angiofibroma. The diagnostic challenge is compounded by its overlapping clinical and histological characteristics with other vascular lesions. Surgical removal proved curative, with no evidence of recurrence after 1 year of follow-up. This case underscores the importance of thorough clinicopathological correlation in establishing an accurate diagnosis and expands the recognized spectrum of ENA, reminding clinicians to include this entity in the differential diagnosis of oral soft tissue masses. Our findings significantly enhance clinical awareness and management.

Dual Antibacterialand Soft-Tissue-Integrative Effectof Combined Strontium Acetate and Silver Nitrate on Peri-Implant Environment:Insights from Multispecies Biofilms and a 3D Coculture Model

Creation of a biological seal and efficient antibacterialqualitiesin the peri-implant environment is essential for the success of dentalimplants. Therefore, novel multifunctional strategies are being developedto address these issues, aiming at the simultaneous improvement oftissue integration and hindering pathological biofilm formation. Inthis study, we investigated the effect of tissue-promotive strontiumacetate (SrAc), antibacterial silver nitrate (AgNO3), andtheir combination on oral soft tissue cells and an oral multispeciesbiofilm not only in monoculture setups but also in a three-dimensional(3D) implant-tissue-oral bacterial-biofilm model (INTERbACT model)that takes the naturally occurring interactions into account. Applicationof SrAc led to improved fibroblast migration in the monoculture setting,without impairment of metabolic activity, even upon additional AgNO3 administration. Notably, the combined treatment of SrAc andAgNO3 resulted in a synergistic antibacterial effect duringbiofilm formation as well as on early matured biofilms. Most interestingly,the antibacterial effect of the combined treatment was even furtherenhanced within the coculture setup leading to increased bacterialdeath and decreased biofilm volume. The 3D tissue in the coculturesetup underwent the combined treatment with a notable rise in CCL20and IL-1β levels. Histologically, only the AgNO3-treatedgroups exhibited damage to the integrity of the epithelial barrier.Therefore, the results of this study demonstrated promising dual antibacterialand tissue-integrative characteristics of combined AgNO3 and SrAc in the dental implant environment. Additionally, the studyemphasizes the importance of considering naturally occurring tissue–bacteriainteractions for reliable in vitro testing of novelimplant materials.

Exploring the Non-Toxic Therapeutic Potential of Dioscorea communis in Combating Oral Pathogenic Bacteria and Their Effects on Hard and Soft Oral Tissues.

Background/Objectives: Gingivitis and dental caries are oral diseases resulting from bacterial accumulation in dental plaque, leading to inflammation, tissue destruction and the demineralization of tooth structures. Dioscorea communis, due to its anti-inflammatory and antimicrobial properties, could be a new treatment candidate. Methods: This study evaluated the preventive and therapeutic effect of a D. communis berry juice paste, formulated at 3% and 7% concentrations, on gingivitis and dental caries, in 55 male SKH-hr2 hairless mice. Gingivitis and dental caries were induced by ligation of the upper left incisor and the paste was applied topically three times daily, five days a week. Treatment efficacy was assessed through clinical examinations, photo-documentation, histopathological analysis and FT-IR spectroscopy. Results/Conclusions: Preventive administration of D. communis 7% significantly delayed disease onset, while therapeutic effects on established conditions were limited. Both concentrations were non-toxic to gingival tissues and dental structures.

Prevalence of oral diseases among adolescents in prisons in Taiwan: a population-based study

ObjectiveThe aim of this study was to estimate the prevalence of oral disease in Taiwanese adolescent prisoners.MethodsWe included 1,610 participants from the National Health Insurance (NHI) Program. Outcomes were measured using the clinical version of the International Classification of Diseases, Ninth Revision (ICD-9-CM).ResultsThe prevalence rate of oral diseases among adolescent prisoners in Taiwan was 29.38%, which was slightly higher among male (30.02%) adolescent prisoners than among female (27.34%) adolescent prisoners (p = 0.316). Among all the patients diagnosed with oral diseases, the top three disease categories were hard tissues of teeth diseases (19.01%), gingival and periodontal diseases (7.70%), and diseases of the oral soft tissues, excluding lesions specific for the gingiva and tongue (4.16%).ConclusionOral disease is not rare among Taiwanese adolescent prisoners. Early prevention and appropriate treatments are important. It is also essential to explore sex-specific needs for this subpopulation.

Tri-Layer Citrate-Based Hydroxyapatite Composite Scaffold Promoting Osteogenesis and Gingival Tissue Regeneration for Periodontal Bone Defect Repair.

Periodontal bone defect (PBD) treatment involving oral soft and hard tissues is complicated and high requirements for regenerated materials. Besides osteogenic effects, the materials are also required to have the function of barrier soft tissues and promote wound healing. Citrate is reported to promote bone formation through enhanced osteoinductivity and facilitate wound healing by enabling phased angiogenesis. Herein, a novel tri-layered citrate-based hydroxyapatite (Ci-HA) composite scaffold that serves as a bone substitute is developed by "one-pot" method for PBD treatment. It is found that Ci-HA degradation products can promote the osteoblastic differentiation of rat bone mesenchymal stem cells and human periodontal ligament stem cells and upregulate angiogenesis-related gene expression in human gingival fibroblasts. Moreover, 3D remodeling in vitro shows that an intermediate layer of tri-layered Ci-HA composite scaffold acts as an optimal barrier. In vivo evaluation of Ci-HA in a rat periodontal intrabony three-wall defect model shows significantly increased bone formation with markedly enhanced osseointegration and better wound healing properties similarly with commercial Bio-Oss bone powder and Bio-Gide membrane. Thus, the novel tri-layered Ci-HA composite scaffold with high biocompatibility may represent a promising biomaterial with multi-effective bone regeneration, barrier effect, and wound healing capacity in the treatment of PBD.

Efficacy of concentrated growth factor in the surgical treatment of postanaesthetic palatal necrosis.

Although uncommon, adverse local and systemic reactions to local anesthetic administration can occurred. The postanesthetic necrosis is a rare complication which usually involved the palatal region. The wound healing is a complex process regulated by a range of growth factors. Concentrated growth factor (CGF) is the latest generation of autologous platelet concentrate which has been widely used in the repair and regeneration of oral soft and hard tissue. The study aims to analyses the clinical benefit of CGF in the treatment of postanesthetic necrosis of the hard palate. The study included all patients referred to the Department of Maxillo-Facial Surgery, Marche University General Hospital, between 2020 and 2022, for aseptic ulcer of the hard palate due to local anesthetic administration. For each patient, the surgical debridement of the necrotic tissue and a peripheral blood sample were performed. The CGF was obtained by a centrifuge device and sutured in the affected site. The greasy gauze, placed to prevent the matrix detachment, was removed seven days post-operative. Seven patients (M:F=4:3, mean age 48.6±5.1 years) were enrolled in the study. After 7 days post-operative, no infection, discomfort, or dysphagia were reported. Moreover, 4 patients (57%) showed a total re-epithelialization, while 3 patients (43%) with larger defects, displayed a partial re-epithelialization. A complete recovery occurred in all cases 15 days after surgery. CFG represents a promising potential preparation for the oral mucosa wound healing able to promote the granular tissue formation and the mucosa re-epithelialization.