Opuntia Ficus-indica Research Articles

Ultrasound-assisted extraction and encapsulation of betalain from prickly pear: Process optimization, in-vitro digestive stability, and development of functional gummies

The study aimed to extract and encapsulate betalain pigment from prickly pear (Opuntia ficus-indica) using ultrasound-assisted extraction and eco-friendly glycerol. Subsequent analysis encompassed assessing its thermal stability, shelf-life, bio-accessibility, and biological properties. The process optimization employed Response Surface Methodology (RSM), focusing on glycerol concentration (20–50 %), sample to solvent ratio (1:10–1:20), temperature (30–60 °C), and time (10–30 min). Optimal conditions were determined as 23.15 % glycerol, 1:10 sample to solvent ratio, 10.43 min treatment time, and 31.15 °C temperature. Under these conditions, betalain content reached 858.28 mg/L with a 93.76 % encapsulation efficiency. Thermal stability tests (80–180 °C; 30 & 60 min) showed degradation of betalain with higher temperatures and longer durations, affecting the visual aspect (ΔE) of the pigment. Encapsulated betalain exhibited favorable shelf stability, with optimal storage life of 404.27 days at 4 °C in amber conditions, compared to 271.99 days at 4 °C without amber, 141.92 days at 25 °C without amber, and 134.22 days at 25 °C with amber. Bio-accessibility of encapsulated betalain was significantly higher (2.05 ± 0.03 %) than conventionally extracted pigment (1.03 ± 0.09 %). The encapsulated pigment displayed strong anti-inflammatory properties in dosages of 2–20 µL, with no cytotoxic effects. Additionally, incorporation into gummies was successful and visually approved by sensory panellists. Glycerol proved to be a green encapsulating agent for betalain, offering high shelf life and bio-accessibility, making it suitable for food industry applications. The encapsulated pigment demonstrated robust thermal stability and shelf life, making it suitable for food industry applications. This study highlights glycerol’s potential as a sustainable alternative for natural pigment extraction.

Identification and molecular docking of xanthine oxidase and α-glucosidase inhibitors in Opuntia ficus-indica fruit.

Opuntia ficus-indica fruit (OFI) is rich in bioactive compounds, which can promote human health. In this work, the purified OFI extract was prepared from OFI and its bioactivities were investigated. Xanthine oxidase (XOD) and α-glucosidase (α-Glu) inhibitors of the purified OFI extract were screened and identified by bio-affinity ultrafiltration combined with UPLC-QTRAP-MS/MS technology. The inhibitory effect of these inhibitors on enzymes were verified, and the potential mechanism of action and binding sites of inhibitors with enzymes were revealed based on molecular docking. The results showed that the total phenolic content of the purified OFI extract was 355.03mg GAE/g DW, which had excellent antioxidant activity. Additionally, the extract had a certain inhibitory effect on XOD (IC50=199.00±0.14µg/mL) and α-Glu (IC50=159.67±0.01µg/mL). Seven XOD inhibitors and eight α-Glu inhibitors were identified. Furthermore, XOD and α-Glu inhibition experiments in vitro confirmed that inhibitors such as chlorogenic acid, taxifolin, and naringenin had significant inhibitory effects on XOD and α-Glu. The molecular docking results indicated that inhibitors could bind to the corresponding enzymes and had strong binding force. These findings demonstratethat OFI contains potential substances for the treatment of hyperuricemia and hyperglycemia.

Efficacy of Opuntia ficus indica mucilage coating on cell wall integrity and shelf-life of banana (Musa spp.) fruit stored at retail condition

Efficacy of <i>Opuntia ficus indica</i> mucilage coating on cell wall integrity and shelf-life of banana (<i>Musa spp</i>.) fruit stored at retail condition

A Novel Foodstuff Mixture Improves the Gut-Liver Axis in MASLD Mice and the Gut Microbiota in Overweight/Obese Patients.

Microbial community control is crucial for maintaining homeostasis of the gut-liver axis in metabolic dysfunction-associated steatotic liver disease (MASLD). Here, we show that supplementation with a mixture of Mexican foodstuffs (MexMix)-Opuntia ficus indica (nopal), Theobroma cacao (cocoa) and Acheta domesticus (crickets)-enriches several beneficial taxa in MASLD mice and overweight/obese humans. Thus, MexMix induces an important prebiotic effect. In mice, a restoration of intestinal health was observed due to the increased short-chain fatty acids (SCFAs) and intestinal crypt depth, Ocln and Cldn1 expression, and decreased Il6 and Tnfa expression. MexMix significantly reduced steatosis in the mice's liver and modified the expression of 1668 genes. By PCR, we corroborated a Tnfa and Pparg decrease, and a Cat and Sod increase. In addition, MexMix increased the hepatic NRF2 nuclear translocation and miRNA-34a, miRNA-103, and miRNA-33 decline. In overweight/obese humans, MexMix improved the body image satisfaction and reduced the fat intake. These findings indicate that this new food formulation has potential as a therapeutic approach to treat conditions associated with excessive consumption of fats and sugars.

Development of biodegradable film from cactus (Opuntia Ficus Indica) mucilage loaded with acid-leached kaolin as filler

Nowadays, substituting petroleum-based plastics with biodegradable polymers made from polysaccharides loaded with various reinforcing materials has recently gained attention due to the impact of conventional plastics wastes. In this study, polysaccharidic mucilage from Ethiopian cactus (Opuntia Ficus Indica) was derived using microwave-assisted extraction technique to develop biodegradable polymers that were inexpensive, readily available, simple to make, and ecofriendly. The effect of microwave power 300–800 W, solid-liquid (cactus-sodium hydroxide solution) ratio 1:5–1:25, sodium hydroxide concentration 0.1–0.8 mol/L, and extraction time 2–10 min on mucilage extraction were studied and the maximum yield of mucilage was attained at optimized parameters of 506 W, 1:20, 0.606 mol/L, and 9.5 min, respectively. Biodegradable polymers made with mucilage alone have poor mechanical characteristics and are thermally unstable. Thus, to overcome the stated problems, glycerol as a plasticizer and acid-leached kaolin crosslinked with urea as a reinforcing material were used. Moreover, the effect of acid-leached kaolin and glycerol on the physico-chemical properties of the films was studied, and a maximum tensile strength of 6.74 MPa with 18.45 % elongation at break, thermally improved biodegradability of 26 %, were attained at 10 % acid-leached kaolin and 20 % glycerol crosslinking with 2 % urea. But the maximum degradability of 53.5 % was attained at 30 % glycerol content. The control and reinforced biodegradable films were characterized using TGA, FTIR, SEM, and XRD to determine the thermal, functional group, morphology, and crystallinity of the bioplastics, respectively. These biodegradable plastics may be used for packaging application.

Coagulant and flocculant effect of Opuntia ficus-indica in water treatment

One of the most relevant processes in potable and wastewater treatment plants is coagulation-flocculation, which gives rise to the destabilization of dissolving solids, for the subsequent generation of flocs using a coagulating agent. Aluminum sulfate (Al2(SO4)3) is the most used coagulant internationally, due to its efficiency and low cost. However, its utilization can leave a residue of aluminum in water and bring with it a variety of health problems due to its bioaccumulative characteristics, implying both the deterioration of ecosystems and problems for human health. In this regard, it is necessary to establish alternatives that avoid the permanence of this heavy metal in water, so that the mucilage of Opuntia ficus-indica becomes a natural coagulant of great interest. Likewise, it is a sustainable alternative for water treatment, given its characteristics of biodegradability, low toxicity, commercial cost, relative abundance, renewability, and high efficiency, allowing a low generation of sludge, which can be biodegraded through a fair treatment with a high valuation spectrum

ACTIVATED CARBON PREPARED FROM SOFTWOOD LIGNOCELLULOSIC BIOMASS – OPUNTIA FICUS INDICA CORDS TO REMOVE AN ANIONIC DYE

In this work, lignocellulosic biomass, namely, Opuntia ficus indica cords (OFIC), was selected as a renewable resource for the production of activated carbon. The preparation conditions of chemically activated carbon (AC) samples were the following: activation temperature – 600 °C, heating rate – 10 °C min-1, activation time – 60 min, and OFIC/H3PO4 impregnation ratio – 1/2. The properties of OFIC and activated carbon were determined by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), Boehm’s method, and pHzpc. AC showed heterogeneous surface and acidic characteristics with a pHZPC of 2.61. OFIC and AC were used as adsorbents to remove the anionic dye Red Bemacid (RB) from aqueous solutions. OFIC was used as a reference for comparison with the prepared AC. The results showed that the experimental data fitted very well with the pseudo-second-order nonlinear model of both adsorbents, with 120 min as equilibrium time and the nonlinear isotherm models of three parameters (Sips and Redlich-Peterson isotherms) were selected as the best fitting ones. The thermodynamic parameters indicated that the adsorption process was spontaneous and endothermic for the AC/RB dye system.

Characterization of mexican Opuntia ficus indica cladode and bioactive compound profile. Oxidative stress resistance and anti-adipogenic effect in Caenorhabditis elegans

Characterization of mexican Opuntia ficus indica cladode and bioactive compound profile. Oxidative stress resistance and anti-adipogenic effect in Caenorhabditis elegans

Cladodes of Opuntia ficus-indica (L.) Mill. Possess Important Beneficial Properties Dependent on Their Different Stages of Maturity.

Opuntia ficus-indica (L.) Mill. belongs to the Cactaceae family and the genus Opuntia; it is a succulent plant that adapts to extreme climatic conditions. The aerial part of the plant consists of the cladodes, morphological changes of branches that appear green, are covered with thorns, and are essential to reduce excessive perspiration of water. The composition of cladodes is very varied, and the main constituents are water, fibers, polysaccharides, proteins, fatty acids, vitamins, sterols, minerals, and polyphenols. Polyphenols are responsible for many beneficial activities for human health, such as antioxidant, anti-inflammatory, anticancer, and nutritional properties. The purpose of this manuscript was to compare the properties of cladodes belonging to the same plant but with different stages of maturity. Relative extracts were tested both in vitro and on a cell line and antioxidant and anti-apoptotic properties were found. The antioxidant activity was tested by the Oxygen Radical Absorbance Capacity (ORAC) test, the 1,1-diphenyl-2-picrylhydrazil (DPPH) test, and the measurement of cellular accumulation of reactive oxygen species (ROS). Anti-apoptotic activity was evaluated by the annexin/PI assay and measurement of caspases 9 and 3 expression. The results obtained showed that the extracts considered possess antioxidant and anti-apoptotic properties. However, the different stages of maturity of cladodes are essential for the performance of both functions. In addition, important variations were made in the dissolution of the extracts that brought greater safety in their use. In conclusion, this manuscript provides further information on cladodes of Opuntia ficus-indica, which can be used as adjuvants in many human pathologies.

Efficacy of herbal extracts in the management of cactus pest, Dactylopius opuntiae (Hemiptera: Dactylopiidae) in Tigray Region, Ethiopia

Cactus (Opuntia ficus-indica) is a drought-resistant plant and the source of food and income for many farmers during the rainy season, and the only option to feed livestock during the dry seasons, especially in the southern part of the Tigray region of Ethiopia. The region is currently suffering from a damaging parasitoid of cactus called Dactylopius opuntiae- ficus biotype. An experimental study was conducted from August 2022 to February 2023 to investigate the efficacy of herbal extracts against cochineal insects. The experiment was conducted on the insecticidal property of methanol extracts of Solanum linnaenum, Euphorbia tirucalli, Nerium oleander, Tephrosia vogelii, Calpurnia aurea, Argemone Mexicana, Datura stramonium and Ricinus communis. Herbs were extracted, and their efficacy and LC50 were determined. Phytochemical screening for secondary metabolites was performed. There was a statistically significant difference in the mean mortality of insects across the levels of the herbs (F (8, 71) = 57.812, p = 0.0001, ƞ2 = 0.867) and the experimental extract concentrations (F (1, 71) = 4198.3, p = 0.0001, ƞ2 = 0.983). The individual extracts of S. linnaenum and N. oleander caused average mortality rates of 99.3% and 97.4%, respectively, at 5% extract concentration. Tannins and saponins were the major metabolites found in the extracts. The present study demonstrated the promising insecticidal efficacy of herbal extracts against the damaging cacti pest D. opuntia-ficus biotype, providing insights into sustainable pest control strategies for protecting vital cactus resources in the Tigray region.

Effect of seed's geographical origin on cactus oil physico-chemical characteristics, oxidative stability, and antioxidant activity

The aim of this study was the valorisation of cactus (or prickly pear, Opuntia ficus-indica) seeds growing in six different regions of Morocco. Moisture, proteins, lipids profile, total polyphenols content, oxidative stability, and antioxidant activity were investigated. The Folin-Ciocalteu test highlighted the abundant presence of phenolic compounds (165 to 225 mg EAG/100 g of extract) and a significant antioxidant capacity against DPPH free radicals. The seeds contained protein (7–9.25%) and lipids (2.7–5%). Cactus oil quality indices such as acidity and peroxide value were below 1.2% and 10 mEq.O2/kg, respectively. GC analysis revealed that linoleic and oleic acid percentages ranged from 57.1 to 63.8%, and 13.5 to 18.7%, respectively. Cactus seed oil was rich in tocopherols (500–680 mg/kg) and phytosterols (8000–11,100 mg/kg) with a predominance of γ-tocopherols and β-sitosterol. Triacylglycerols, fatty acids and sterols composition showed small variation depending on the geographical origin, while the individual tocopherol profile was significantly influenced.

Effect of removing the spring flush and nitrogen and phosphorus fertilization on the late ripening of cactus pear Opuntia ficus-indica (L.) Mill

The present work aims to study the effect of removing the spring flush (scozzolatura practice) and nitrogen and phosphorus fertilization on fruit yield and quality of the late fruiting of cactus pear Opuntia ficus-indica (L.) Mill. To meet this objective, an experiment was carried out on an adult plantation of ‘Aissa’ cv in the Agadir area. Treatments of fertilization used are: T0 (not fertilized plot: contains only the soil reserve in NP: 40-20 kg N-P ha-1), T1: 226 kg N ha-1, T2: 114 kg P2O5 ha-1 and T3: 226-114 kg N-P2O5 ha-1. The whole spring flush was removed during the full flowering stage (50% flowers in bloom); it was practiced on April 20, 2019. Obtained results showed that removing the spring flush led to a second flush of flowering (reflowering) 46 days after the spring flowering and an off-season late fruiting 68 days after the seasonal fruiting. The scozzolatura practice also has improved the fruit size and taste and its content in sugars, and reduced the number of seeds per fruit. Nitrogenous and phosphor-nitrogenous fertilizations improved the emission of shoots and flowers, fruit yield and size of scozzolatured and not scozzolaturated plants and their effect is more important on scozzolatured plants than on not scoscozzolatured ones. However, phosphorous fertilization alone does not affect these phenological and yield parameters and nitrogen and phosphorus fertilizations have no effect on the organoleptic parameters of the fruits (the rate of juice and sugars and the pH and acidity titratable of the juice).

Usefulness of Opuntia spp. on the Management of Obesity and Its Metabolic Co-Morbidities.

The plants of the Opuntia genus mainly grow in arid and semi-arid climates. Although the highest variety of wild species is found in Mexico, Opuntia spp. is widely distributed throughout the world. Extracts of these cacti have been described as important sources of bioactive substances that can have beneficial properties for the prevention and treatment of certain metabolic disorders. The objective of this review is to summarise the presently available knowledge regarding Opuntia ficus-indica (nopal or prickly pear), and some other species (O. streptacantha and O. robusta) on obesity and several metabolic complications. Current data show that Opuntia ficus-indica products used in preclinical studies have a significant capacity to prevent, at least partially, obesity and certain derived co-morbidities. On this subject, the potential beneficial effects of Opuntia are related to a reduction in oxidative stress and inflammation markers. Nevertheless, clinical studies have evidenced that the effects are highly contingent upon the experimental design. Moreover, the bioactive compound composition of nopal extracts has not been reported. As a result, there is a lack of information to elucidate the mechanisms of action responsible for the observed effects. Accordingly, further studies are needed to demonstrate whether Opuntia products can represent an effective tool to prevent and/or manage body weight and some metabolic disorders.

Reduction of Malnutrition Related to Unsafe Water Consumption in Developing Countries: Potabilization of Surface Water and Traditional Well Water, with Plant Extracts.

The consumption of unsafe water in rural areas is a real public health problem in developing countries. This situation mainly affects children under five years of age and causes several deaths and many cases of malnutrition every year. The objective of this study was to evaluate and optimize the capacity of four local plant extracts in the potabilization of unsafe water. Thus, Moringa oleifera and Boscia senegalensis seeds, or Aloe vera and Opuntia ficus-indica mucilages were prepared in a solution and applied during a jar test as biocoagulants and bioflocculants on three raw water samples of 82.3 NTU, 549.8 NTU and 796.9 NTU. After treatment results showed that 0.9 g/L of Moringa biocoagulant or 1 g/L of Boscia biocoagulant applied with 0.4 mL of Aloe vera bioflocculant or 0.6 mL of Opuntia ficus-indica bioflocculant reduced the turbidity of each water sample to values less than 5 NTU after only 15 min of decanting. Moreover, the sanitary quality of the water treated by these different extracts showed a perfect conformity of the physicochemical and microbiological parameters with the standards of acceptability in drinking water decreed by the World Health Organization. Thus, the application of these local plant extracts has made it possible to considerably improve the quality of unsafe water in record time. Their popularization could be an alternative in the fight against malnutrition related to the consumption of unsafe water, especially in rural areas.

Cactus pear (Opuntia ficus-indica) cladode extracts as a growth medium for Lactobacillus species: the case of Lactobacillus reuteri and Lactobacillus mali

Cactus pear (Opuntia ficus-indica) cladode extracts as a growth medium for Lactobacillus species: the case of Lactobacillus reuteri and Lactobacillus mali

Hemicellulose‐Based Nanofibers Decorated In Situ with Silver Nanoparticles for Antibacterial Applications

AbstractThere is growing evidence that composites made up of nanomaterials and plant extracts are effective in combating drug‐resistant pathogens, which are increasing globally. In this study, a hemicellulose extract from cactus pads (Opuntia ficus indica) is blended with poly(vinyl) acetate and electrospun into fine nanofibers. Further, the electrospun nanofibers are encapsulated with silver nanoparticles (AgNPs), in situ, to form a nanocomposite, whose surface morphology are characterized using a scanning electron microscope (SEM). The SEM images showed smooth nanofiber strands (average diameter = 240 nm), with AgNPs randomly dispersed on them. The antibacterial activity of the nanocomposite is tested against five bacterial species. The results obtained showed that the nanonomposite has strong inhibitory effects against Gram‐positive and Gram‐negative bacteria, whereas the pristine nanofibers showed minimal or no antibacterial activity. This study demonstrates i) successful electrospinning of hemicellulose extracts into fine nanofibers and ii) encapsulation with AgNPs to produce a rigid nanocomposite with antimicrobial characteristics for various applications, especially those that require protection against pathogenic bacteria.

Storage Conditions of Refrigerated Prickly Pears in Small Processing Industries

Prickly pears (Opuntia ficus-indica), which are well known for their beneficial properties for human health, are the subject of many studies due to their high content of bioactive compounds. However, the presence of spines on the fruit’s surface is a factor that limits consumption. Therefore, the viability and nutritional quality of peeled and packaged white, orange, and pink prickly pears were studied during storage. Refrigerated whole fruits stored at 8 °C and 85% RH for 0, 1, 2, 3, or 4 weeks were electrically peeled and packaged with micro-perforated film. At each time point, their microbial quality; physical parameters, such as hardness, texture, and color; and chemical parameters, including pH, titratable acidity, total soluble solids content, sugar content, ascorbic acid content, antioxidant capacity, and total phenol content, were analyzed. The mesophilic aerobic counts were lower than the values established by Spanish legislation (7 log (CFU/g f.w.)) until day 8 after packaging (or until day 6 after 4 weeks of storage). The hardness, texture, pH, sugar content, ascorbic acid content, and antioxidant capacity decreased significantly between day 0 and day 8 after packaging, independently of the number of weeks for which the whole fruits had been refrigerated previously. Furthermore, the changes in the parameters analyzed for whole fruits during storage were less remarkable. The evaluation of the sensory features was positive throughout the preserved period. The minimally processed prickly pears retained suitable microbial, nutritional, and sensory qualities when the whole fruits had been refrigerated for 1, 2, 3, or 4 weeks, facilitating their management in small processing companies.

Discriminating among Alternative Dressing Solutions for Cereal Seed Treatment: Effect on Germination and Seedling Vigor of Durum Wheat

A prompt seed germination and emergence coupled with an excellent seedling vigor are highly desired features to ensure perfect crop establishment and subsequent vegetative growth. Seed dressing with pesticides represents the most common technology for enhancing seed performance after sowing, while little is known about biostimulant seed dressing. This practice could play a fundamental role in developing new sustainable starter fertilization for cereals. The enhancement of germination and seedling vigor of durum wheat seeds (Triticum turgidum L. subsp. durum (Desf.) Husn) was the main target of this research. The experiment took place in a germination cabinet under controlled environmental conditions, settled at the constant temperature of 10 °C and under dark conditions for 8 days. The different seed dressings, sprayed on the seeds, were composed by a combination of a fungicide and different biostimulants. Coleoptile and root length, as well as biomass, were significantly increased by the different biostimulants, compared to the control. As for germination traits, seeds treated with Codium fragile and Opuntia ficus-indica extracts, containing phytohormones and different nutrients, showed a final germination (96%) significantly higher than the one obtained with the control treatment (86%). These results show that treating seeds with a suitable dressing solution can greatly improve the germination features and seedling vigor of durum wheat. This can help the crop to withstand future stresses, especially in early stages, and possibly increase the grain yield with a reduction in agrochemicals. However, the combination of the substances used in the present study rarely showed a synergistic effect on the tested variable.

Fungal causal agents of the Black Spot of the cactus (Opuntia ficus-indica) in Colima, Mexico

Objective/Background: The prickly pear cactus (Opuntia ficus-indica) holds significant economic, social, and cultural importance in Mexico. However, it is recurrently affected by Black Spot disease (BS), caused by various phytopathogenic fungi. Identifying the causal agents of BS in commercial prickly pear crops is crucial for efficient agronomic management of the disease. The objective of this study was to identify the phytopathogenic fungi responsible for BS in prickly pear plantations in the Colima state, Mexico. Materials and Methods: Fifty cladodes from 50 plants exhibiting BS symptoms were collected from commercial plantations in Colima. The pathogenicity of the isolated fungi was verified using Koch’s postulates, and those causing the most severe BS symptoms were molecularly identified. Results: Thirty-five fungi were isolated from plants with BS symptoms, of which 20 exhibited distinct mycelial growth. Only six fungi induced BS symptoms; three of them were responsible for severe symptoms in cladodes: Alternaria alternata, Corynespora cassiicola, and Neoscytalidium dimidiatum. Conclusion: BS is caused by various phytopathogenic fungi, but this is the first report of C. cassiicola and N. dimidiatum as causal agents of BS in prickly pear cactus.

Fatty acids characterisation by GC-MS, antiglycation effect at multiple stages and protection of erythrocytes cells from oxidative damage induced by glycation of albumin of Opuntia ficus-indica (L.) Mill seed oil cultivated in Eastern Morocco: Experimental and computational approaches

Ethnopharmacological relevanceOpuntia ficus-indica (L.) Mill is frequently observed in the Moroccan traditional medicinal system, where these approaches are employed to mitigate the onset of diabetes and the subsequent complications it may entail. Aim of the studyThe aim of this research was to examine the effectiveness of Opuntia ficus-indica seed oil in preventing diabetic complications. Specifically, the study assessed its ability to counteract glycation at various stages, protected red blood cells from the harmful effects of glycated albumin, and inhibited pancreatic lipase digestive enzymes to understand its potential antihyperglycemic properties. Additionally, the study aimed to identify the chemical components responsible for these effects, evaluate antioxidant and anti-inflammatory properties, and conduct computational investigations such as molecular docking. Materials and methodsThe assessement of Opuntia ficus-indica seed oil antiglycation properties involved co-incubating the extract oil with a bovine serum albumin-glucose glycation model. The study investigated various stages of glycation, incorporating fructosamine (inceptive stage), protein carbonyls (intermediate stage), and AGEs (late stage). Additionally, measurement of β-amyloid aggregation of albumin was performed using Congo red, which is specific to amyloid structures. Additionally, the evaluation of oil's safeguarding effect on erythrocytes against toxicity induced by glycated albumin included the measurement of erythrocyte hemolysis, lipid peroxidation, reduced glutathione. The fatty acid of Opuntia ficus-indica seed oil were analyzed using Gas Chromatography-Mass Spectrometry (GC–MS). The in vitro evaluation of antihyperglycemic activity involved the use of pancreatic lipase enzyme, while the assessement of antioxidant capability was carried out through the utilization of the ABTS and FRAP methods. The in vitro assessement of the denaturation of albumin activity was also conducted. In conjunction with the experimental outcomes, computational investigations were undertaken, specifically employing ADMET (absorption, distribution, metabolism, excretion, and toxicity) analysis. Furthermore, molecular docking was utilized to predict antioxidant and antiglycation mechanisms based on protein targets. ResultsIn vitro glycation assays, Opuntia ficus-indica seed oil displayed targeted inhibitory effects at multiple distinct stages. Within erythrocytes, in addition to mitigating hemolysis and lipid peroxidation induced by glycated albumin. GC-MS investigation revealed a richness of fatty acids and the most abundant compounds are Linoleic acid (36.59%), Palmitic acid (20.84%) and Oleic acid (19.33%) respectively. The findings of antioxidant ability showed a remarkable activity on FRAP and ABTS radicals. This oil showed a pronounced inhibitory impact (p < 0.001) on pancreatic lipase enzyme. It also exerted a notibale inhibition of albumin denaturation, in vitro. ConclusionThe identified results were supported by the abundant compounds of fatty acids unveiled through GC-MS analysis, along with the computational investigation and molecular docking.