Optimal Conditions For Production Research Articles

Production of xylooligosaccharides from different pretreated Camellia oleifera shell with crude enzyme from Aspergillus fumigatus

Production of xylooligosaccharides (XOS) from Camellia oleifera shell (COS) using the crude enzyme from Aspergillus fumigatus (A. fumigatus) was investigated. COS was subjected to different pretreatments, namely thermal autoclaving, combination of diluted acid, diluted alkali or concentrated alkali with thermal autoclaving, microwave-assited ethanol pretreatment. The results showed that the combination of diluted alkali with thermal autoclaving is the best pretreatment for XOS production. The optimal conditions for enzymatic xylooligosaccharides production from the best pretreatment are: temperature 50 °C, pH 4.5, substrate concentration 8% (m/V), enzymatic hydrolysis time 9 h, and enzyme dose of 40 IU/g substrate. A maximum yield of 17.08 ± 0.67 g/L of XOS was obtained under the above optimum conditions for the best pretreatment.

Partial purification of linoleic acid isomerase enzyme from Lactobacillus paracasei bacteria isolated from milk.

Conjugated Linoleic Acid (CLA) has attracted the attention of many researchers, especially that of microbial origin due to its biological importance to the consumer. The current study aims to extract LA Isomerase enzyme from Lactobacillus paracasei bacteria from milk and to use the enzyme in the production of CLA. Selective media, including MRS and MRS-Dagatose, were used in isolating local strains. The selected bacterial isolates were tested for their ability to produce LA-Isomerase enzyme. The isolate with high enzymatic activity was selected. After extraction and partial purification of the enzyme, the optimal conditions for the production of conjugated fatty acid were studied, and the reaction products were diagnosed using GC-MS technology. It was found that 11 isolates have the ability to produce CLA at different concentrations, H1 isolate showed the highest production of conjugated fatty acid at a concentration of 120.45 g.ml-1, this isolate was selected as the source for enzyme extraction. The enzymatic activity of the crude extract and partially purified with ammonium sulfate was estimated using color methods at wavelength of 233 nm. The effect of the optimum conditions (pH, temperature, linoleic acid concentration and enzyme concentration) on the CLA product was studied using the partially purified LA Isomerase enzyme, the optimum conditions for production were 6.5, 45 °C, 100 μg.ml-1 and 0.7 ml, respectively. The GC-MS technique showed the presence of a number of reaction products that are isomers of conjugated linoleic acid (C9T11, T9T12, T10C12) with different concentrations.

Investigation of The Use of Calcium Naphthalene Sulfonate as A Binder in Magnesite Spinel Brick Production and Determination of Optimum Conditions

Refractory materials are indispensable for industries working with high temperatures. Magnesite spinel refractory bricks are used in the cement industry, and calcium lignosulfonate is used as a binder in their production. Due to forest fires and similar reasons in recent years, there is a problem in the supply of calcium lignosulfonate raw materials. In this study, research was conducted on alternative binders to be used in the production of magnesite spinel bricks and the optimum conditions for production with the selected binder were determined. Some of the binders are calcium naphthalene sulfonate, sodium naphthalene sulfonate, magnesium oxide, magnesium sulfate, and molasses. As a result of preliminary tests, it was observed that the most successful results were obtained with calcium naphthalene sulfonate. The optimum conditions for the production of magnesite spinel refractory bricks using calcium naphthalene sulfonate were found by the Taguchi method. As parameters, seawater sintered magnesite in 4 different fractions (A: 3-5 mm, B: 1-3 mm, C: 0-1 mm and D: Powder) and Sinter Spinel in 2 different fractions (E: 3-5 mm and F: 1-3 mm) in total, 6 parameters were selected and an L16 (44x22) Taguchi orthogonal array design was created for this. Volume weight, water absorption, porosity, and strength tests were performed on the samples obtained. Accordingly, taking into account the strength values, the optimum conditions were determined as A1, B1, C2, D4, E1 and F2. Under these conditions, the estimated strength value was calculated as 84.24 N/mm2 and the experimental value was 83.85 N/mm2.

Optimizing solid-state fermentation for metabolite enrichment by Aspergillus tamarii on rice bran and wheat

Globalization has led to an increase in the global population and drug resistance, necessitating the development of novel pharmaceuticals. Fungi represent a promising source for new drugs, producing a diverse array of secondary metabolites, some of which are integral to clinically essential drugs. This study aimed to determine the optimal conditions for enhancing metabolite production by the Indonesian fungal strain Aspergillus tamarii (IPBCC 880066) when cultivated on solid media, specifically rice bran and wheat. The optimal fermentation conditions, including moisture content, growth temperature, and incubation duration, were determined based on the number of spots observed on thin-layer chromatography plates. Results indicated that the optimum conditions for metabolite production were a 40% moisture content, a growth temperature of 25°C, and a 14-day incubation period. Subsequent UPLC-MS/MS analysis identified 22 metabolites produced by A. tamarii on rice bran and 20 metabolites on wheat. Notably, two compounds, N-(2-hydroxypropyl)-2-methylacrylamide and N6-[(benzyloxy)carbonyl]-L-lysine, were detected in both rice bran and wheat fermented by A. tamarii. Some of the identified metabolites have the potential to be applied in the pharmaceutical industry. In conclusion, our study emphasizes A. tamarii’s efficacy in diverse metabolite production under optimized conditions, providing valuable insights for maximizing fungal metabolite production in potential pharmaceutical applications.

Comparative genomic analysis and optimization of astaxanthin production of Rhodotorula paludigena TL35-5 and Rhodotorula sampaioana PL61-2.

Astaxanthin is a powerful antioxidant known to enhance skin, cardiovascular, eye, and brain health. In this study, the genome insights and astaxanthin production of two newly isolated astaxanthin-producing yeasts (TL35-5 and PL61-2) were evaluated and compared. Based on their phenotypic and genotypic characteristics, TL35-5 and PL61-2 were identified as basidiomycetous yeasts belonging to Rhodotorula paludigena and Rhodotorula sampaioana, respectively. To optimize astaxanthin production, the effects of cultural medium composition and cultivation conditions were examined. The optimal conditions for astaxanthin production in R. paludigena TL35-5 involved cultivation in AP medium containing 10 g/L glucose as the sole carbon source, supplemented with 1.92 g/L potassium nitrate, pH 6.5, and incubation at 20°C for 3 days with shaking at 200 rpm. For R. sampaioana PL61-2, the optimal medium composition for astaxanthin production consisted of AP medium with 40 g/L glucose, supplemented with 0.67 g/L urea, pH 7.5, and the fermentation was carried out at 20°C for 3 days with agitating at 200 rpm. Under their optimal conditions, R. paludigena TL35-5 and R. sampaioana PL61-2 gave the highest astaxanthin yields of 3.689 ± 0.031 and 4.680 ± 0.019 mg/L, respectively. The genome of TL35-5 was 20,982,417 bp in length, with a GC content of 64.20%. A total of 6,789 protein-encoding genes were predicted. Similarly, the genome of PL61-2 was 21,374,169 bp long, with a GC content of 64.88%. It contained 6,802 predicted protein-encoding genes. Furthermore, all essential genes involved in astaxanthin biosynthesis, including CrtE, CrtYB, CrtI, CrtS, and CrtR, were identified in both R. paludigena TL35-5 and R. sampaioana PL61-2, providing evidence for their ability to produce astaxanthin.

Efficiency of cotton and fiber wetting in cotton ginning plants

This article provides a comprehensive examination of humidification technologies and points within ginneries, with a specific focus on the moisture content of the produced fiber. Several key aspects are covered, ranging from the assessment of existing humidification technologies to the determination of moisture levels in the fiber across different ginneries. Additionally, the article delves into the changes in moisture content observed in various cotton components during technological processes, particularly in fiber bales utilized in production at the spinning factory "OSBORN TEXTILE," along with associated challenges. By examining these facets, the article provides valuable insights into the intricacies of managing moisture content in cotton processing, from ginneries to spinning factories. The findings contribute to a better understanding of the role of humidification technologies in maintaining optimal conditions for cotton fiber production and subsequent textile manufacturing processes. The identification of challenges and vices allows for targeted improvements and optimizations in the industry's practices.

Vitamin B12 production in solubilized protein extract of bioprocessed wheat bran with Propionibacterium freudenreichii

Wheat bran, a nutrient-rich byproduct of wheat milling, has the potential to be used for the production of vitamin B12 (B12) in order to fortify plant-based foods that are lacking in this vitamin. This study aimed to investigate B12 production in the soluble extract of bioprocessed wheat bran using Propionibacterium freudenreichii. The optimal conditions for B12 production were found to be a bioprocessing time of 24 h, along with the use of a commercial starter culture of lactic acid bacteria and yeast, with pH adjusted from 4.0 to 6.4. The highest B12 production (55 ng/mL) was achieved within three days of fermentation. Cobalt supplementation (as CoCl2 or food-grade yeast extract) resulted in up to four-fold increase in B12 production. Similar results (50–70 ng/mL) were obtained when the processes were scaled up, with bioprocessing involving up to 15 kg and B12 fermentation involving up to 5 L, demonstrating the robustness of the procedure. Microbiological safety was ensured with the absence of Enterobacteriaceae in the extracts after bioprocessing and B12 fermentation. Overall, this study highlights the potential of under-utilized wheat bran as a raw material for producing B12-enriched bran extract, which can be used to fortify plant-based products in various food applications.

Valorization of bio-oil distillation residue through co-pyrolysis with moso bamboo to prepare biochar: Optimization study on effects of blend ratio, pyrolysis temperature, and residence time

In view of the high energy consumption and pollutant emissions in the cement production industry, biomass as the renewable carbon-neutrality energy is promisingly conducive to remitting cement carbon dioxide emissions and simultaneously realizing waste-to-energy initiatives. In this study, bio-oil distillation residue (DR), with high carbon content and heat value, is co-pyrolyzed with moso bamboo (MB) to optimize the bulk and combustion properties of biochar, aiming at yielding bio-fuel as an alternative fuel to replace coals for energy and heat supply in cement industry. The impacts of three primary process parameters on the carbonization reaction were investigated, while the parametric interactions were further estimated by using response surface methodology (RSM) to optimize the ultimate, proximate and fuel characteristics of biochars. The results showed the co-pyrolysis synergies between bio-oil distillation residue and moso bamboo endowed the maximum biochar yield of 44.62% and the maximum difference biochar yield of 7.56%. Meanwhile, the experimental carbon content, fixed carbon content and high heat value (HHV) were regulated through coupling parametric distribution and further increased by 1.96%, 2.59% and 1.23 MJ/kg, respectively. The parametric responses on combustion characteristics of biochar based on thermogravimetric analysis were predicted, and the optimal carbonization conditions for biochar production were also summarized. This study would offer an optimal biochar production scheme to adaptively yield coal-like biochar for reducing anthropogenic carbon dioxide emissions in cement industry.

Exploration and characterization of a newly isolated bacterium, Enterobacter quasihormaechei strain BDIFST24001, capable of producing rhamnolipid biosurfactant for oil remediation.

Biosurfactants are naturally occurring compounds synthesized by micro-organisms that increasingly attract attention due to both their living area and application in various industries. In this study, we explore and characterize a novel bacterium, Enterobacter quasihormaechei strain BDIFST24001, isolated for its ability to produce rhamnolipid biosurfactants, with the aim of facilitating oil remediation processes. The isolation of this bacterium was carried out using Luria-Bertani (LB) broth media from environmental samples collected from oil-contaminated sites in Dhaka City. Screening tests, including the oil spreading method and drop collapse assay, were conducted to identify potential biosurfactant-producing strains, leading to the selection of E. quasihormaechei strain BDIFST24001 based on its favourable performance. Subsequent molecular identification revealed a high similarity of the strain's 16S rRNA gene to E. quasihormaechei, which was corroborated through phylogenetic analysis. Further analysis of the biosurfactant produced by this strain indicated its rhamnolipid nature, as confirmed by FT-IR spectroscopy. The rhamnolipids exhibited promising surface-active properties, including a significant reduction in surface tension and emulsification activity, as evidenced by surface tension measurements and emulsification index assays. Optimization studies revealed that the optimal conditions for rhamnolipid production by E. quasihormaechei strain BDIFST24001 were a temperature of 37 °C, pH 10.0 and salinity of 4 %. The rhamnolipids produced by this strain demonstrated effective oil remediation capabilities, as observed through controlled experiments using petrol oil. The rhamnolipids effectively reduced the surface tension of the oil-water interface, facilitating the dispersion and emulsification of the oil phase in water. Overall, our findings highlight the potential of E. quasihormaechei strain BDIFST24001 as a promising candidate for biosurfactant-mediated oil spill cleanup and environmental remediation efforts.

Beauveria Bassiana Amylase-Polygalacturonase Production Using Lignocellulosic Biomass and Application in Juice Processing.

The search for sources of industrial biocatalysts, which are non-pathogenic and can utilise cheap nutrient sources, has been a continuous endeavour in the ~ 7 billion USD enzyme industry. Beauveria bassiana, an endophytic fungal entomopathogen, is non-pathogenic and possesses the potential to secrete various bioproducts while utilising readily available lignocellulosic biomass. This study investigated the optimised production of two glycosyl hydrolases, amylase and polygalacturonase, by B. bassiana while utilising readily available agricultural residues. Subsequently, the industrial potential of the enzymes in the clarification of fruit juice was evaluated. Initially, seven agro residues were screened for the concomitant production of amylase and polygalacturonase by B. bassiana SAN01. Subsequently, statistical optimisation tools, Plackett Burman Design (PBD) and Central Composite Design (CCD), were employed for the optimisation of enzyme production. The enzyme mixture was partially purified and applied in the clarification of pineapple juice. The production of B. bassiana SAN01 amylase and polygalacturonase was found to be maximal while utilising wheat bran. Subsequent to PBD and CCD optimisation, the optimal conditions for enzyme production were identified to be at 30 °C, pH 6.0 and wheat bran concentration of ~40 g.L-1. Under these optimised conditions, heightened production levels of 34.82 and 51.05 U.mL-1 were recorded for amylase and polygalacturonase, respectively, which were 179% and 187% of the initial unoptimised levels. In addition, the most effective clarification of the juice (~90%) was observed at 35 °C after an incubation time of 120 min with no significant effect on the pH and total dissolved solids. B. bassiana, a well-known biocontrol agent, was shown to produce amylase and polygalacturonase using readily available agricultural residues for the first time. These enzyme production levels are the highest for these enzymes from any known endophytic fungal entomopathogen. This study further demonstrates the potential applicability of B. bassiana in other industrial processes besides its widespread use as a biopesticide.

Investigating the Optimization of Magnetic Biochar Production from Rubber Seed Shells for Enhanced Cr(VI) Removal Efficiency

This study aimed to utilize agricultural and produce low-cost magnetic biochar from rubber-seed shells using ferric chloride (FeCl3) as a transition metal. The study employs Response Surface Methodology (RSM) based on the Box-Behnken Design (BBD) to determine optimal production conditions for removing chromium (Cr(VI)). The effect of preparation conditions such as pyrolysis temperature (500-700 °C), duration (90-180 min), and impregnation (1-3 M) on the produced magnetic biochar was examined. The optimal condition was demonstrated based on yield percentage and Cr(VI) removal efficiencies. The study revealed that the optimal conditions for producing magnetic biochar from rubber seed shells were a pyrolysis temperature of 580 °C, a pyrolysis time of 130 min, and a FeCl3 concentration of 3 M. Under these conditions, a yield of 48.63% was achieved, and the removal efficiencies for Cr(VI) were 41.29%. This research suggests that utilizing agricultural waste products from rubber seed shells may be a viable and economical method for producing magnetic biochar, which can serve as an efficient adsorption agent.

PTIMIZATION AND CHARACTERIZATION OF USED COOKING OIL FOR BIODIESEL PRODUCTION USING RESPONSE SURFACE METHODOLOGY

This study focuses on optimizing and characterizing alkali-catalyzed biodiesel production from used cooking oil. Transesterification using potassium hydroxide (KOH) and methanol, followed by solvent-solvent extraction, yielded biodiesel. Physicochemical analysis of the used cooking oil revealed an acid value of 29 mgNaOH/g, free fatty acid (FFA) value of 14.5, and density of 0.91 g/cm3. The high FFA content suggests the use of a heterogeneous catalyst. Optimization parameters included alcohol-to-oil ratio, catalyst concentration, reaction temperature, and time, employing Response Surface Methodology (RSM) based on Central Composite Design (CCD). Optimal conditions for biodiesel production were determined at a reaction temperature of 60°C, a reaction time of 60 minutes, 0.3g KOH catalyst concentration, and a 3:20 methanol-to-oil ratio, predicting a 100% yield. Physiochemical properties of the produced biodiesel indicated specific gravity and pH values of 0.891 and 7.60, respectively. Biodiesel blends (B100, B80, and B20) exhibited specific gravity and pH values of 0.891, 0.842, and 0.839, and 7.60, 7.81, and 5.5, respectively. Comparative analysis with diesel suggests the biodiesel's suitability for standalone or blended use in diesel engines. Characterization involved physicochemical analysis, Fourier Transform Infrared Spectroscopy (FTIR), and Thin Layer Chromatography (TLC). Overall, the optimized process presented a viable and efficient approach to producing biodiesel from used cooking oil with favourable fuel properties

Optimisation of Polyhydroxy Butyrate Production by Lysinibacillus fusiformis and Metabacillus indicus isolated from Spent Engine-oil Contaminated Soil

This study isolated bacteria from spent engine oil-contaminated soil and optimized their production of polyhydroxybutyrate (PHB), a biodegradable polymer belonging to the polyesters classes that are of interest as bioderived and biodegradable plastics. Out of 12 bacterial isolates (species of Bacillus, Pseudomonas, Staphylococcus and Lactobacillus) recovered from the spent engine oil contaminated soils, and screened for their capacity to accumulate polyhydroxybutyrate (PHB), only two bacterial isolates (Lysinibacillus fusiformis and Metabacillus indicus), showed significant PHB production. L. fusiformis produced PHB at a concentration of 1.5 g/L, while M. indicus produced PHB at a concentration of 1.0 g/L. Optimal production conditions included a temperature of 35°C, agitation speed of 100 rpm, neutral pH of 7.0, glucose as the carbon source, and peptone as the nitrogen source. Gas chromatography-mass spectrometry confirmed the presence of PHB in the extracted samples, with hexadecanoic acid methyl ester identified as the predominant peak. These findings highlight the potential of bacteria from engine oil-contaminated soil as efficient PHB producers and contribute to the development of sustainable and biodegradable plastics.

Comprehensive approach for assessing, managing, and Monitoring Asphaltene Deposition and inhibitor utilization in oil wells

This study addresses the challenges of managing asphaltene precipitation in oil well production, aiming to establish a rapid computational approach for production management based on individual well productivity. The methodology involves modeling the impact of inhibitors on tubing diameter and incorporating well and reservoir performance. Validation using real-world data reveals optimal production conditions related to Asphaltene Onset Pressure (AOP) and Bubble Point Pressure (Pb) in relation to inhibitor location. The study emphasizes the importance of positioning AOP above the perforation interval for operable conditions and observes the impact of asphaltene deposition on wellhead pressure. To address increased bottomhole drawdown pressure, a production rate regulatory program is developed. The research offers a novel computational approach for managing asphaltene precipitation, grounded in real-world data, and provides insights into optimizing production rates while ensuring flow assurance in oil well operations. Additionally, strategic recommendations for field development are proposed, including acid/hydraulic fracturing and deviated horizontal/bilateral wells drilling to mitigate bottomhole drawdown pressure.

Optimization of the Centrifugal Spinning Parameters to Prepare Poly(butylene succinate) Nanofibers Mats for Aerosol Filter Applications.

Air pollution is becoming a serious issue because it negatively impacts the quality of life. One of the first most useful self-defense approaches against air pollution are face masks. Typically made of non-renewable petroleum-based polymers, these masks are harmful to the environment, and they are mostly disposable. Poly(butylene succinate) (PBS) is regarded as one of the most promising materials because of its exceptional processability and regulated biodegradability in a range of applications. In this regard, nanofiber-based face masks are becoming more and more popular because of their small pores, light weight, and excellent filtration capabilities. Centrifugal spinning (CS) provides an alternative method for producing nanofibers from various materials at high speeds and low costs. This current study aimed to investigate the effect of processing parameters on the resultant PBS fiber morphology. Following that, the usability of PBS nonwoven as a filter media was investigated. The effects of solution concentration, rotating speed, and needle size have been examined using a three-factorial Box-Behnken experimental design. The results revealed that PBS concentration had a substantial influence on fiber diameter, with a minimum fiber diameter of 172 nm attained under optimum production conditions compared to the anticipated values of 166 nm. It has been demonstrated that the desired function and the Box-Behnken design are useful instruments for predicting the process parameters involved in the production of PBS nanofibers. PBS filters can achieve an excellent efficiency of more than 98% with a pressure drop of 238 Pa at a flow rate of 85 L/min. The disposable PBS filter media was able to return to nature after use via hydrolysis processes. The speed and cost-effectiveness of the CS process, as well as the environmentally benign characteristics of the PBS polymer, may all contribute considerably to the development of new-age filters.

Comparative evaluation of the effect of ultrasonic pretreatment at different temperatures on enhancing anaerobic digestion of rumen wastes

The aim of this study was to determine the most appropriate treatment for the duration of ultrasonic pretreatment at two different temperatures in the process of anaerobic digestion (AD) of ruminal contents in the slaughterhouse. The experiments were performed at the Golshan industrial slaughterhouse in Lorestan province. In this study, after homogenizing the rumen contents of different animals, the effect of ultrasonic pretreatment time at three different intervals of 10, 20, and 30 min at two different temperatures of 30 °C and 40 °C was investigated to determine the effect of variables related to the trend of changes in methane, total biogas, and daily biogas production. The Taguchi L18 method was used to investigate the process conditions and achieve optimal production conditions for biogas methane. The results showed that with an increase in pretreatment time up to 30 min and the temperature up to 40 °C, the production of biogas and methane also increased. The highest amount of methane, total biogas, and daily biogas produced was related to the use of ultrasonic pretreatment for 30 min at 40 °C with 50.66 % methane, total biogas production of 332.66 mL/gVS, and the best performance compared to other treatments in the shortest possible time. Therefore, the use of ultrasonic pretreatment over longer periods and at higher temperatures improves the AD process and produces more total methane and biogas. Finally, this research presented equations for the production of biogas and methane at a time of 10–30 min and a temperature of 30–40 °C, which showed high accuracy.

Biological Ammonia Production via Anaerobic Fermentation of Soy Meal Protein.

Conventional ammonia production methods, notably the energy-intensive Haber-Bosch process, are costly and contribute substantially to about 2% of the world's CO2 emissions. This study focuses on the biological approach to convert protein to ammonia via hyper-ammonia-producing bacteria (HAB) fermentation. A consortium of ruminal microbes was employed in this work to ferment soybean meal protein under varying processing conditions. The parameters investigated included pH (7-11), inoculum concentrations (1-10%), substrate concentrations (5-20%), and fermentation time (0-168 h). Optimal conditions for microbial growth and biological ammonia production were observed at pH 7, fermentation duration of 72 h, inoculum concentration of 10%, and substrate concentration of 10%. ~8000 mg/L biological ammonia was produced following HAB fermentation. By leveraging the capabilities of rumen HAB, this study contributes to the ongoing efforts to develop environmentally friendly processes for ammonia production that will mitigate both economic and environmental concerns associated with traditional methods.

Viscosity influence on kinetics parameters in dry anaerobic digestion

The viscosity that governs mass exchanges is a determining parameter of dry digestion kinetics. This observation leads to the measurement of viscosity influence on key kinetic parameters of digestion such as microbial growth, degradation of COD, biogas production and its CH4 quality. Thus, for limit viscosities at the beginning of digestion in batch mode at 37 °C and 55 °C of potato residues of 18–35 % TS content, the evolution of kinetic parameters is observed. The results show strong microbial growth for 4.39 × 10−2 Pa s and 7.5 × 10−2 Pa s in digestion at 37 °C and 4.12 × 10−2 Pa s in digestion at 55 °C. The reduction of COD is optimal for 7.50 × 10−2 Pa s at 37 °C and 6.57 × 10−2 Pa s at 55 °C. Biogas production is higher for 7.5 × 10−2 Pa s at 37 °C and 4.12 × 10−2 Pa s at 55 °C. Finally, the CH4 content of biogas is higher for 4.39 × 10−2 Pa s and 4.12 × 10−2 Pa s at 37 °C and 55 °C, respectively. These results show that optimal conditions for microbial growth and CH4 production are obtained with the 22 % TS scenario giving a viscosity of 4.39 × 10−2 Pa s at 37 °C and 4.12 × 10−2 Pa s at 55 °C. These results confirm the importance of the viscosity in biochemical kinetics in dry digestion.

In situ chlorpyrifos (CPF) degradation by Acrobeloides maximus: Insights from chromatographic analysis

The objective of this study was to evaluate the efficiency of nematodes in zooremediation of chlorpyrifos (CPF), an organophosphate pesticide. The nematode population Acrobeloides maximus (A. maximus) was employed for bioremediation, converting CPF into non-toxic residues. Optimal growth conditions for mass production of A. maximus were achieved by maintaining a temperature of 25 °C, pH 8, and supplementing the culture medium with plant nutrients. The nematodes were then immobilized within sodium alginate beads. The efficacy of the degradation process was assessed using various analytical techniques, including UV-Visible spectroscopy, HPTLC, FTIR, and LC-MS, confirming the successful breakdown of CPF. The bioreactor demonstrated a complete degradation efficiency of CPF exceeding 99%. Additionally, LC-MS analysis was conducted to elucidate the degradation pathway based on the formation of intermediates. These results underscore the potential of A. maximus as a sustainable organism for addressing environmental contamination arising from CPF pesticide.

Production of Dextran from Domestic Weissella cibaria Isolated

Dextran is an exopolysaccharide (EPS) of bacterial origin that is readily available in the commercial market. that is used in various industrial applications as an adjuvant, emulsifier, carrier, and stabilizer in the food and biomedical industries. Industrial production of dextran is carried out by fermentation in sucrose-rich media. Studies to optimize dextran production have shown that dextran yield varies depending on specific production conditions. This study aimed to generate dextrans Lactobacillus species were obtained from the vaginal and stool samples of healthy infants. The mucosal technique was employed to determine the biosynthesis of dextran from various isolates of Lactobacillus spp. colony assay and confirmed by isopropanol precipitation. The effects of various parameters such as sucrose concentration, nitrogen concentration, temperature, incubation time, pH, and inoculum size were studied to optimize the maximum dextran yield. In the present study, Weissella cibaria may produce dextran. Optimal conditions for dextran production are a 48-hour incubation at The experimental conditions involved maintaining a temperature of 37°C, with a sugar concentration of 10%, and a yeast extract concentration of 0.5%. The inoculum volume was set at 4%, and the pH level was maintained at 6.5. The experiment was conducted under aerobic conditions. The obtained value for dextran dry weight was 520 mg/100 ml.