Opposite-sex Dizygotic Twin Pairs Research Articles

Dementia risk in women higher in same-sex than opposite-sex twins.

Hormones may be one possible mechanism underlying sex differences in dementia incidence. We examined whether presumed differential prenatal hormone milieu is related to dementia risk by comparing dementia rates in same- and opposite-sex dizygotic twin pairs in male and female twins. The sample comprised 43,254 individuals from dizygotic twin pairs aged 60 and older from the Swedish Twin Registry. Survival analyses were conducted separately for females and males. Female twins from opposite-sex pairs had significantly lower dementia risk than female twins from same-sex pairs, but the differences emerged only after age 70 (hazard ratio = 0.64, P = 0.004). Results were not explained by postnatal risk factors for dementia, and no interaction between twin type and apolipoprotein E (APOE) ε4 was found. Male twins from same-sex versus opposite-sex pairs did not differ significantly. The results suggest that relatively masculine prenatal hormone milieus correlate with lower dementia risk in females.

Sex differences in the pathways to symptoms of alcohol use disorder: a study of opposite-sex twin pairs.

We sought to develop an empirical, broad-based developmental model for sex differences in risk for symptoms of alcohol use disorders, here called alcohol problems (APs). We assessed 18 risk factors in 5 developmental tiers in both members of 1,377 opposite-sex dizygotic twin pairs from the Virginia population-based twin registry. Analyses were conducted by structural modeling, examining within-pair differences. The best-fitting model explained 73% of the variance in men and 71% in women for last year AP. Forty-nine percent of paths differed significantly across sexes. Ten variables had appreciably different predictive effects on AP in males versus females. Three were stronger in females: familial risk, early-onset anxiety disorders, and nicotine dependence. Seven predictors had a stronger total effect in males: novelty seeking, conduct disorder, childhood sexual abuse, parental loss, neuroticism, low self-esteem, and low marital satisfaction. In a co-twin control design, which matches sisters and brothers on genetic and familial-environmental background, we found numerous sex differences in predictors of last year AP. Factors that were more prominent in men and in women were diverse, reflecting both internalizing and externalizing psychopathology. The model was slightly more successful at predicting AP in men than in women.

Sex differences in the pathways to major depression: a study of opposite-sex twin pairs.

The authors sought to clarify the nature of sex differences in the etiologic pathways to major depression. Retrospective and prospective assessments of 20 developmentally organized risk factors and the occurrence of past-year major depression were conducted at two waves of personal interviews at least 12 months apart in 1,057 opposite-sex dizygotic twin pairs from a population-based register. Analyses were conducted by structural modeling, examining within-pair differences. Sixty percent of all paths in the best-fit model exhibited sex differences. Eleven of the 20 risk factors differed across sexes in their impact on liability to major depression. Five had a greater impact in women: parental warmth, neuroticism, divorce, social support, and marital satisfaction. Six had a greater impact in men: childhood sexual abuse, conduct disorder, drug abuse, prior history of major depression, and distal and dependent proximal stressful life events. The life event categories responsible for the stronger effect in males were financial, occupational, and legal in nature. In a co-twin control design, which matches sisters and brothers on genetic and familial-environmental background, personality and failures in interpersonal relationships played a stronger etiologic role in major depression for women than for men. Externalizing psychopathology, prior depression, and specific "instrumental" classes of acute stressors were more important in the etiologic pathway to major depression for men. The results are consistent with previously proposed typologies of major depression that suggest two subtypes that differ in prevalence in women (deficiencies in caring relationships and interpersonal loss) and men (failures to achieve expected goals, with lowered self-worth).

The Genetics of Sexuality and Aggression (GSA) Twin Samples in Finland

The Genetics of Sexuality and Aggression (GSA) project was launched at the Abo Akademi University in Turku, Finland in 2005 and has so far undertaken two major population-based data collections involving twins and siblings of twins. To date, it consists of about 14,000 individuals (including 1,147 informative monozygotic twin pairs, 1,042 informative same-sex dizygotic twin pairs, 741 informative opposite-sex dizygotic twin pairs). Participants have been recruited through the Central Population Registry of Finland and were 18-49 years of age at the time of the data collections. Saliva samples for DNA genotyping (n = 4,278) and testosterone analyses (n = 1,168) were collected in 2006. The primary focus of the data collections has been on sexuality (both sexual functioning and sexual behavior) and aggressive behavior. This paper provides an overview of the data collections as well as an outline of the phenotypes and biological data assembled within the project. A detailed overview of publications can be found at the project's Web site: http://www.cebg.fi/.

A Twin Study of Computer Anxiety in Turkish Adolescents

The present study investigated computer anxiety within a sample of Turkish twins aged 10-18. A total of 185 twin-pairs participated in the study. Of the twins, 64 pairs (34.6 percent) were monozygotic (MZ) and 121 pairs (65.4 percent) were dizygotic (DZ). Of the 121 DZ twins, 54 pairs (44.63 percent) were same-sex twins and 67 pairs (55.37 percent) were opposite-sex twins. Computer anxiety was assessed using Computer Anxiety Rating Scale-Turkish Version (CARS-TV), one of the three main scales of "Measuring Technophobia Instruments" developed by Rosen and Weil. The results of paired t test comparisons showed no significant differences in MZ and same-sex DZ twin-pairs' levels of computer anxiety. On the other hand, a significant difference was found in opposite-sex DZ twin-pairs' level of computer anxiety. Interesting enough, males appeared to be more computer anxious than their female co-twins. In the present study, using Falconer's formula, heritability estimate for computer anxiety was derived from correlations based on MZ and DZ twins' mean scores on CARS-TV. The results showed that 57 percent of the variance in computer anxiety was from genetics and 41.5 percent was from nonshared environmental factors. Shared environmental influence, on the other hand, was very small and negligible. Interpretations of results and potential directions for future research are presented.

The Role of Attention-Deficit/hyperactivity Disorder in the Association between Verbal Ability and Conduct Disorder

Although there is clear evidence that low verbal ability is a risk factor for conduct disorder (CD), some researchers have questioned whether this association is due to the common comorbidity between attention-deficit/hyperactivity disorder (ADHD) and CD. The present study examined the association among verbal ability, ADHD, and CD in a genetically informative sample in order to examine the role of genes and/or environmental influences shared in common with ADHD on the covariation between verbal ability and CD. Participants were 2744 adolescents from the Center for Antisocial Drug Dependence (CADD), and included 360 monozygotic (MZ) female twin pairs, 221 dizygotic (DZ) female twin pairs, 297 MZ male twin pairs, 220 DZ male twin pairs, and 274 opposite-sex DZ twin pairs. The Diagnostic Interview Schedule for Children (DISC-IV) was used to assess lifetime symptoms of ADHD and CD. Verbal ability was assessed via the Vocabulary subtest of the Wechsler Adult Intelligence Scale III (WAIS-III) for individuals over the age of 16 and the Vocabulary subtest of the Wechsler Intelligence Scale for Children III (WISC-III) for individuals under the age of 16. There was a small but significant negative covariance between verbal ability and CD and between verbal ability and ADHD. Results also suggest that the covariation between verbal ability and CD is due to influences shared in common with ADHD.

Sex Differences in the Relationship Between Social Support and Risk for Major Depression: A Longitudinal Study of Opposite-Sex Twin Pairs

Compared to men, women have larger and more intimate social networks and higher rates of major depression. Prior studies have suggested that women are more sensitive to the depressogenic effects of low social support, but most of these studies had substantial methodologic limitations. In two interview waves at least 1 year apart, 1,057 pairs of opposite-sex dizygotic twin pairs ascertained from a population-based register were assessed. The authors predicted risk of major depression in the year before the wave 2 interview from levels of social support assessed at wave 1. Women reported higher levels of global social support than their twin brothers. Global social support at wave 1 predicted risk for major depression at wave 2 significantly more strongly in female than in male members of these pairs, and the same effect was seen when the analysis controlled for the history of major depression in the year prior to wave 1. Women were more sensitive than men to the depressogenic effects of low levels of social support, particularly from the co-twin, other relatives, parents, and spouses. Levels of social support did not explain the sex difference in risk for major depression. Emotionally supportive social relationships are substantially more protective against major depression for women than for men. While these effects cannot explain sex effects on the prevalence of major depression, they do suggest important sex differences in pathways of risk. Clarification of the nature of the causal links between low social support and depression in women is needed.

Similarity of childhood experiences and personality resemblance in monozygotic and dizygotic twins: a test of the equal environments assumption

The equal environments assumption suggests that the environment does not contribute more strongly to the similarity of monozygotic (MZ) compared to dizygotic (DZ) twins. It was tested in 525 MZ, 200 same-sex DZ, and 68 opposite-sex DZ twin pairs. Almost 80% of the twins were female, and their age varied widely around a mean of 32 years. Similarity of twin environments was assessed by a self-report questionnaire on childhood experiences, and personality resemblance was measured using the self-report and peer-report versions of the NEO-Five-Factor Inventory. MZ twins reported more similar experiences than DZ twins and were also more alike in personality. Across twin pairs, however, treatment similarity was unrelated to personality resemblance, except in the combined group of MZ and DZ twins. These results are consistent with the equal-environments assumption.

Widespread pain among 11-year-old Finnish twin pairs.

To examine the prevalence of widespread musculoskeletal pain (WSP) symptoms in 11-year-old Finnish twins and to determine the relative role of genetic and environmental factors in the etiology of WSP. Data on current pain items were collected from 1995 to 1998 from a national sample of Finnish families with 11-year-old twins born between 1984 and 1987. The presence of WSP was determined using a validated questionnaire method. Pairwise similarity was computed for 583 monozygotic (MZ) pairs, 588 same-sex dizygotic (DZ) pairs, and 618 opposite-sex DZ twin pairs. Variance components for genetic and environmental factors were estimated using biometric structural equation modeling techniques. The prevalence of WSP was 9.9%, with no sex difference. The majority of twin pairs with WSP were discordant. The tetrachoric correlations for male MZ (r = 0.38), male DZ (r = 0.37), female MZ (r = 0.59), female DZ (r = 0.54), and opposite-sex pairs (r = 0.43) showed little difference by zygosity. Female pairs were more concordant than male pairs among both MZ and DZ twins. Biometric model-fitting indicated that genetic factors did not account for the pattern of twin similarity. Among boys 35%, and among girls 56%, of the variation in liability to WSP could be attributed to shared familial environmental effects. The remainder was attributed to unshared environmental effects. Genetic factors seem to play at most a minor role in WSP in 11-year-old twins, and environmental factors shared by family members account for a substantial proportion of the variability in WSP.

A twin study of gender-influenced individual differences in general cognitive ability

In order to test the hypothesis that the etiology of individual differences in general cognitive ability differs in males and females, full-scale IQ data from a sample of 426 twin pairs [211 monozygotic (MZ) twin pairs, 128 same-sex dizygotic (DZ) twin pairs, and 87 opposite-sex DZ twin pairs], with a mean age of 11.85 years, were fitted to a structural equation model of sex-limitation. Resulting estimates of heritability did not differ significantly in males and females (0.69 and 0.41, respectively), and corresponding estimates of the variance due to shared-environmental influences (0.10 and 0.32) were not significantly different from zero. Moreover, the estimate of the between-sex genetic correlation was one, with a 95% confidence interval of 0.61–1.00. Thus, individual differences in general cognitive ability appear to be due substantially to common genetic influences in males and females.

Genetic and Environmental Influences on Personality: A Study of Twins Reared Together Using the Self‐ and Peer Report NEO‐FFI Scales

ABSTRACT Previous behavior‐genetic research on personality has been almost exclusively based on self‐report questionnaire measures. The purpose of this research was to measure personality constructs via self‐ and peer reports on the items of the NEO Five‐Factor Inventory (Costa & McCrae, 1989). The sample included 660 monozygotic and 200 same sex and 104 opposite sex dizygotic twin pairs. We collected self‐ and two independent peer reports for each of the twins. Our analysis of self‐report data replicates earlier findings of a substantial genetic influence on the Big Five (h2= .42 to .56). We also found this influence for peer reports. Our results validate findings based solely on self‐reports. However, estimates of genetic contributions to phenotypic variance were substantially higher when based on peer reports (h2= .51 to .81) or self‐ and peer reports (h2= .66 to .79) because these data allowed us to separate error variance from variance due to nonshared environmental in‐fluences. Correlations between self‐ and peer reports reflected the same genetic influences to a much higher extent than identical environmental effects.

Sex-typed play in opposite-sex twins

To examine possible prenatal hormonal influences on sex-typed play, we compared girls with a boy co-twin to girls with a girl co-twin and girls with an older brother. In opposite-sex dizygotic twin pairs, the uterine environment may allow transfer of testosterone from the male to the female fetus. Singletons with an older brother provide a control for shared social environment. Girls aged 3 to 8 years (N = 91) were observed playing with sex-typed toys, and mothers completed questionnaires about the child's activities. Contrary to expectation, girls with a boy co-twin did not spend more time playing with boys' toys than girls with a girl co-twin or girls with an older brother. Although these results might suggest that normal variations in hormones do not contribute to within-sex variations in childhood activities, they combine with other work to suggest factors to consider in evaluating hormonal influences on human behavior, including level and timing of exposure.

Sex differences and nonadditivity in heritability of the Multidimensional Personality Questionnaire Scales.

A sex-limitation model was applied to the Multidimensional Personality Questionnaire (MPQ) data from the Minnesota Twin Family Registry. The sample included 626 monozygotic (MZ) twin pairs, 517 same-sex dizygotic (DZ) twin pairs, and 114 opposite-sex DZ twin pairs ranging in age from 27 to 64 years (mean age = 37.8). In addition, whenever possible, family members of the twins were also assessed: 1,690 spouses, 495 parents, 322 siblings, and 535 offspring. The goals of the present investigation were to analyze the covariance matrices to determine whether (a) there was evidence for sex limitation of heritability of the MPQ scales and (b) there was evidence for nonadditive genetic influences on these scales. Significant sex differences in heritability were found for 3 of the scales: Alienation, Control, and Absorption. In addition, evidence for dominance was found for all scales except Traditionalism and Absorption.

Sex differences and nonadditivity in heritability of the Multidimensional Personality Questionnaire Scales.

A sex-limitation model was applied to the Multidimensional Personality Questionnaire (MPQ) data from the Minnesota Twin Family Registry. The sample included 626 monozygotic (MZ) twin pairs, 517 same-sex dizygotic (DZ) twin pairs, and 114 opposite-sex DZ twin pairs ranging in age from 27 to 64 years (mean age = 37.8). In addition, whenever possible, family members of the twins were also assessed: 1,690 spouses, 495 parents, 322 siblings, and 535 offspring. The goals of the present investigation were to analyze the covariance matrices to determine whether (a) there was evidence for sex limitation of heritability of the MPQ scales and (b) there was evidence for nonadditive genetic influences on these scales. Significant sex differences in heritability were found for 3 of the scales: Alienation, Control, and Absorption. In addition, evidence for dominance was found for all scales except Traditionalism and Absorption.

Genetic influences on ambulatory blood pressure patterns: The Medical College of Virginia twin study

The genetic influence of ambulatory blood pressure and heart rate was examined in 38 pairs of monozygotic twins, 17 pairs of same-sex dizygotic twins, and 11 pairs of opposite-sex dizygotic twins, all aged 15 or 17 years. The data were analyzed taking into consideration that the response was multivariate (24-h values) instead of the usual univariate response. The results demonstrated the heritability of ambulatory blood pressure and heart rate. This was true regardless of whether the estimate of heritability involved monozygotic twin pairs compared to same-sex dizygotic twin pairs only, or all dizygotic twin pairs. The time-related intraclass correlation coefficient within each twin classification indicated that the patterns of response within twin pairs correlated more for monozygotic twin pairs than within twin pairs for either set of dizygotic twin pairs. In addition, although the opposite-sex dizygotic twin pairs may have different mean levels of response, they exhibit a similarity of patterns of response akin to that seen within same-sex dizygotic twin pairs.

Familial influences on heart rate, blood pressure, and self-report anxiety responses to stress: results from 100 twin pairs.

Two hundred healthy adolescent to middle-aged individuals (12-44 years, M = 20 years) were tested in a standardized stress protocol. These individuals comprised 20 monozygotic female, 20 monozygotic male, 20 same-sex dizygotic female, 20 same-sex dizygotic male, and 20 opposite-sex dizygotic twin pairs. Familial influences on heart rate, blood pressure, and self-report anxiety responses to four different kinds of stressors (Visual-Verbal Test for Conceptual Thought, mental arithmetic, isometric handgrip, cold pressor) were assessed using biometrical genetic model fitting. Evidence of significant genetic effects on resting heart rate and blood pressure was obtained, providing heritability estimates of .65, .63, and .58 for resting heart rate and systolic and diastolic blood pressure, respectively. Cardiovascular reactivity to the Visual-Verbal Test, mental arithmetic, and the cold pressor test appeared to be primarily influenced by genetic and idiosyncratic (nonfamilial) environmental factors, whereas reactivity to handgrip was more related to effects of the family environment. The results of multivariate model fitting suggested that the genetic effects on reactivity were relatively independent of those affecting resting heart rate and blood pressure and that there was significant overlap of genetic influences on heart rate and blood pressure responses to the two active coping tasks.