Oil In Mice Research Articles

Teratogenic effects of Plectranthus fruticosus essential oil in mice

AbstractThe teratogenic potential of Plectranthus fruticosus essential oil was evaluated in Swiss‐OF1 mice injected subcutaneously. They received daily 0, 15, 45 or 135 mg/kg body weight on days 6 to 15 of gestation. A slight maternal toxicity, as evidenced by reduced weight gain and some physical alterations, was observed among treated groups compared with controls. Animals were killed and subjected to uterine examination on day 17 of pregnancy. Plectranthus fruticosus essential oil induced both embryo‐ and feto‐toxicity in the three treated groups, as manifested by a statistically significant increase in the numbers of resorbed and malformed fetuses. The observed malformations were predominantly related to the eyes (anophthalmia).

CNS activity of Mentha rotundifolia and Mentha longifolia essential oil in mice and rats

AbstractThe essential oils of Mentha rotundifolia and Mentha longifolia were analyzed with GC and GC/MS. The major components were rotundifolone (10.4%) and piperitol (57.6%) in M. rotundifolia, and rotundifolone (33.2%) and diosphenol (47.7%) in M. longifolia. M. longifolia essential oil exhibited a stronger CNS depressant effect than that of M. rotundifolia; both essential oils were somnifacients.

Depression of DNA synthesis of thymocytes with dietary autoxidized oils in mice

Depression of DNA synthesis of thymocytes with dietary autoxidized oils in mice

Teratological evaluation of Juniperus sabina essential oil in mice.

Juniperus sabina essential oil was evaluated for its fetotoxic potential on mice. Pregnant dams were injected s.c. (15-45 or 135 mg essential oil/kg body weight) on days 6 to 15 of gestation. They were killed and the uterine contents were examined on day 19 of pregnancy. The fetuses were removed for examination. The dams of the two higher treated groups showed a significant weight loss as compared to controls. An hepatotoxicity was observed among females that resorbed their whole litter, thus indicating a greater susceptibility towards Juniperus sabina essential oil during pregnancy. The essential oil induced, in the three treated groups, an embryotoxicity as manifested by a statistically significant increase in the number of affected litters; but no fetotoxicity.

Decreases in spleen weight and blood leucocytes number with long-term feeding of oxidized oil in mice.

Decreases in spleen weight and blood leucocytes number with long-term feeding of oxidized oil in mice.

Experimental studies on TDI dermatitis in mice.

Allergic contact dermatitis occurred with 2, 4-toluene diisocyanate (TDI) in BALB/c mice. The results were obtained by the measurement of the ear thickness after challenged to the ear with 1% TDI in olive oil in mice previously sensitized with 5% TDI. The ear swelling rate at 48 hr postchallenge in sensitized mice was more than twice of that in unsensitized mice. In BALB/c-nu/nu (nude) mice, delayed type hypersensitivity could not be induced even when a 30% TDI solution was applied. These results suggest that T cells may play an essential role in contact sensitivity to TDI in BALB/c mice.

18 month skin painting study of a mercapto-functional silicone oil in mice.

Thrice weekly application of lambda-mercapto-polydimethylsiloxane to the shaven backs of Swiss mice over an 18 month period did not have an obvious effect on weight gain nor did this material produce systemic toxicity. Lung adenomas and lymphosarcomas were observed upon histopathological examination in both untreated and treated group. Other lesions observed were not thought to be treatment related.

Early cellular changes and circular muscle contraction associated with the induction of decidualization by intrauterine oil in mice

Intrauterine instillations of oil or saline distended the uterus in ovariectomized mice treated with progesterone + oestrogen to sensitize the uterus to a decidualizing stimulus. Saline does not induce decidualization, and therefore uterine distension per se is not the trigger to decidual induction. Oil induces decidualization, but does not involve gross damage to the epithelium, penetration of oil into the stroma or release of epithelial lipid into the stroma. Instillation (oil, saline or sham) induced a contraction of the circular muscles along the length of the uterus which closed the uterine lumen, expelled most of the oil and located the remainder primarily in the antimesometrial cleft of the lumen. Progesterone inhibited longitudinal muscle contraction and facilitated circular muscle contraction. These effects are discussed in relation to the spacing and implantation of blastocysts.

Uterine DNA synthesis and cell proliferation during early decidualization induced by oil in mice.

[3H]Thymidine autoradiography was used to study cell proliferation during decidualization induced by intraluminal oil in ovariectomized mice treated with oestrogen and progesterone. Development of the decidual reaction involves two distinct populations of stromal cells. Periluminal cells start to synthesize DNA 11--15 h after instillation and by 17--20 h, without dividing, differentiate into epithelioid decidual cells which continue to incorporate [3H]thymidine, presumably becoming polyploid. Cells peripheral to this zone also start to synthesize DNA between 11 and 15 h, but at 18.5 h many have divided before differentiating. None of these dividing cells had been arrested in G2. The periluminal and peripheral cells do not appear to differ in their proliferative antecedents.

In vivo studies on the effect of garlic oil in mice

The in vivo effect of garlic oil on protein, glycogen and lipid metabolism as well as on oxidative phosphorylation has been studied in liver and kidneys of mice. The incorporation of [ 14C] leucine into proteins of the liver is virtually unchanged by treatment with garlic oil whereas the labelling of proteins of the kidneys is reduced. Garlic oil enhances the incorporation of [ 14C] glucose into hepatic glycogen, produces a temporary rise in blood glucose levels and depletes liver glycogen. Renal glycogen levels and incorporation of [ 14C] glucose into renal glycogen remain unchanged following garlic oil treatment. The compound has little or no effect on the incorporation of [ 14C] acetate into hepatic and renal lipids. Garlic oil impairs partially the oxidative phosphorylation associated with the oxidation of ascorbate in hepatic mitochondria without affecting noticeably the P/O ratio during the oxidation of glutamate and succinate.

Study on Carcinogenicity of Casting Oils in Mice

Study on Carcinogenicity of Casting Oils in Mice