The article presents the author’s position of studying organizational trust in customs service and specific experience of workplace stress that influences its formation. The study sample involved 77 customs officers in the following categories of customs personnel: customs inspectors and passenger inspectors. The scientific value of this study lies in establishing the empirical referents of the relationship between organizational trust and relief of organizational stress in customs officers at different organizational levels. The article proves that the empirical referents of the interrelation of organizational trust and experience of workplace stress in the customs group 3 are at the mezzo-level of organizational trust (concern from management, supported by indicators of organizational culture in the field of communication and management) and at the micro-level (increasing organizational stress in the forms of activity and productivity, the flexibility of behaviour, acceptance of others’ values and indicators of job satisfaction due to the expressed emotional commitment of these employees to their organization). The practical value of the obtained results allows one to understand the nature and the impact of organizational stress and the level of organizational trust on the effectiveness of customs officers’ professional activities. It also makes it possible to develop a further programme of psychological support for this professional group to improve psychological safety and well-being in the workplace.