Geranium caespitosum is self-compatible, yet selfing decreases both seed number and seed weight compared to outcrossing. A mechanism that inhibits the growth of self pollen would reduce losses in fitness from selfing and could be detected by comparing the number of pollen tubes entering ovules following self- and cross-pollination. Pistils receiving either self- or cross-pollen were each held for 2 h or 24 h and then prepared for fluorescence microscopy. After 2 h, fewer self- than cross-pollen tubes entered ovules. Differences between the number of self- and cross-pollen tubes entering ovules diminished after 24 h, indicating that many self-pollen tubes were delayed, but not excluded, from entering micropyles. Fluorescence microscopy confirmed that many pollen tubes are aborted in a specific region of transmitting tissue within the ovary. Thus, a labile mechanism favors cross-pollen but allows selfing when pollinators or neighboring plants are scarce.
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