Present study was to find out biodiversity status of River Jamuaari and evaluate several effects of herbicide spraying in the river basin. The main objective of this study was to monitored lose of the biodiversity and current land-use activities in Jamuaari river basin and their impacts on the nutrient load in river ecosystem. In India, many researchers have worked on physico-chemical study of rivers (Chaturvedi, 2006) and toxicity of Glyphosate herbicide. Recently scientists have found harmful effects of pesticides on living being (Mose et al; 2008, Gasnier et al; 2009) e.g. cancer, geno-toxicity, endocrine disruption, reproduction. Some research work on the limnological study of river (Kumar & Sinha, 2005), but No complete Physico-chemically, Ecologically and zoogeographically work done prier and tributary basin of Jamuaari river in Sarairanjan block of south Samastipur has not been investigated such manner till now. So this research work was performed. Total five year Phosphate and bicarbonate concentration was the main determinant of the nutrient loads/productivity, and ranged between 30/1 and 90/5 of the total five year change. Five-year water and soil samples confirmed that eutrophication increased with increasing herbicide use, with significant differences between biodiversity of tributary basin and far most sampling sites. The seasonal phosphate and bicarbonate concentration change, were highest in tributary basin (65/5.3,60/3.7,15/1.8, site I, site II and site III ), mainly site I as compared to far most region of river bank (5/2.2,5/1.4,5/5.5, site IV, site V and site VI ).Within five year phosphate and bicarbonate concentration change, were highest in tributary basin (35/0.2,25/1.6,20/0, site I, site II and site III) mainly site l as compared to far most region of river bank(5/0.4,0/0,5/1.4, site IV, site V and site VI) also. The lose of distribution of flora and fauna species as a function of ecosystem has also showed significant differences in the basin as the biodiversity status (rate of biodiversity index change) of the tributary basin.