This study analyzed the relationship between physical activity, diet, and blood sugar levels in patients with diabetes mellitus at the Tambak Wedi Baru Community Health Center, Surabaya, from February to May 2025. The study design used was a correlational analytic design with a cross-sectional approach. The sample consisted of 72 respondents aged 20 to 71 years, who were selected randomly. Univariate analysis focused on a single variable through descriptive statistics, presenting data as numbers, ratios, percentages, or prevalence. Bivariate analysis examined the relationship between two variables using non-parametric statistics, such as the Spearman test, to explore associations between diet, physical activity, and blood sugar levels. Findings revealed significant relationships between physical activity and diet and blood sugar levels (ρ = 0.007; ρ = 0.000). Good physical activity (>1500 MET-min) was associated with normal blood sugar levels, while low physical activity (<600 MET-min) was associated with abnormal blood sugar levels. On the other hand, poor diet (<650 calories) also contributed to high blood sugar levels. This study highlights the importance of multidisciplinary interventions to increase physical activity and healthy eating patterns for the effective management of diabetes mellitus.

This study evaluated the effects of a year-long educational program on adolescents’ knowledge of type 2 diabetes mellitus (T2DM). In this longitudinal study of 643 Arab adolescents (300 boys, 343 girls; mean age 14.8 ± 1.7 years), baseline education sessions were conducted in schools, focusing on T2DM risk factors, development, and the importance of lifestyle modifications. Educational sessions were held every 3 months by certified endocrinologists and nutritionists. Knowledge about T2DM was assessed using the modified Michigan Diabetes Research and Training Center Diabetes knowledge test. A significant increase in T2DM knowledge scores was observed in all participants (40.5 ± 15.2 vs 28.4 ± 15.3, P < .001) after 12 months, with significant improvements for both males and females (P-values < .001). Girls showed a greater increase in knowledge score than boys (P < .001). T2DM participants also improved their knowledge scores significantly (45.4 ± 13.9 vs 28.1 ± 14.6, P < .001). After 12 months, 10.3% of participants with normal glycemia transitioned to prediabetes, and 8.3% progressed to T2DM. Among those with prediabetes at baseline, 87.2% achieved normal blood sugar levels, while 55.0% of T2DM participants at baseline reverted to normal. Health education translated to improved knowledge related to T2DM among adolescents, particularly in girls and those with T2DM. Strengthening health education in schools across Saudi Arabia is advisable to raise awareness and prevention of T2DM.

ABSTRACTBackground and AimsLifestyle and diet influence chronic disease risk through their impact on systemic inflammation. This research evaluated the association among dietary habits, physical activity, and inflammatory biomarkers, specifically glucose and C‐reactive protein (CRP).MethodsThis cross‐sectional study examined lifestyle, sociodemographic, and nutritional indicators using a structured questionnaire, which was adapted from the WHO STEPwise guideline. A total of 200 participants were included based on their attendance for CRP and glucose assays at two health centers between May and September 2023 in Bangladesh.ResultsAmong participants with high fiber intake (> 20 g/day), 22% had normal blood glucose levels and 25.5% showed slightly increased CRP values. In contrast, among those with low fiber intake (< 10 g/day), 28% had pre‐diabetes, 23.5% had diabetes, and a moderate 56% exhibited moderately increased CRP levels. Besides, participants with higher saturated fat intake (> 10 g/day) showed moderate CRP elevations in 50%, pre‐diabetes in 24%, and diabetes in 19.5%, whereas those with lower intake (< 10 g/day) exhibited smaller CRP elevations in 28.5% and normal blood sugar levels in 27.5% of the group. Participants with a habit of rare or once‐weekly physical activity had pre‐diabetes in 38.5%, diabetes in 27%, and moderate CRP elevations in 57% of their group. On the contrary, those reporting regular physical activity demonstrated lower CRP levels in 20.5% and normal sugar levels in 34.5% of their group. Intake of fruit, vegetables, red meat, seafood, sugary drinks, high‐sugar foods, and smoking showed statistically significant associations with inflammatory biomarkers (p < 0.05); however, water intake did not exhibit a similar association. Glucose and CRP levels were found to be significantly associated with age, dietary factors (fiber, saturated fat, red meat, sugary diet), and lifestyle behaviors (physical activity, smoking), whereas sociodemographic factors (gender and education) showed no statistically significant associations.ConclusionThe findings highlight associations between diet and physical activity and inflammatory markers, suggesting the necessity for focused public health interventions.

Background: Pyridoxine-dependent epilepsy due to ALDH7A1 gene mutation is a known, but rare autosomal recessive disorder, presenting with early-onset, refractory seizures. Also, mutations in the RHOBTB2 gene are responsible for a rare developmental and epileptic encephalopathy 64 disorder. We report a neonate with refractory seizures harboring mutations in both the genes. Clinical Description: A 4-day-old female neonate, born to second-degree consanguineous parents, presented with left-sided focal seizures. The antenatal period was uneventful with no birth asphyxia. The elder male sibling had died at 4 years of age due to infantile-onset epilepsy. Examination of the neonate revealed hypotonia and impaired neonatal reflexes. Management and Outcome: Investigations revealed a negative septic screen, normal blood sugar, calcium, and magnesium levels. Magnetic resonance imaging of the brain was normal, and the electroencephalogram showed generalized epilepsy. Seizures persisted despite 4 days of treatment with intravenous phenobarbitone, levetiracetam, and phenytoin. Pyridoxine was added on day 5 of hospitalization, following which there was reduction in seizure frequency over 24 hours and complete cessation over the subsequent 48 hours. Genetic testing revealed a homozygous missense variant of uncertain significance in the ALDH7A1 gene as well as a heterozygous nonsense variant of uncertain significance in the RHOBTB2 gene, both genes showing phenotypic corroboration. Conclusion: Though the addition of pyridoxine may be beneficial in refractory seizures in neonates, genetic analysis may be considered to elicit underlying mutations, especially with a background of consanguinity and positive family history. Our case is unique, showing the coexistence of ALDH7A1 and RHOBTB2 gene variants in a case of neonatal seizures.

IntroductionResearch works relating to the lifestyle of students, non-academic staff, and communities have had considerable attention. However, studies on the lifestyles of university lecturers in Ghana and their association with the occurrence of hypertension and diabetes have received very scanty attention.ObjectiveThis study assesses the modifiable lifestyle practices among lecturers in a Ghanaian university, and how these are associated with the occurrence of hypertension and diabetes.MethodsIt was a cross-sectional study design with a quantitative approach involving 205 lecturers in a Ghanaian university. Data were analyzed using descriptive statistics and regression.ResultsThe study found that 50.73% and 33.66% of lecturers were overweight (25.0 < 30 Kg/m2) and Obese (<30 Kg/m2) respectively. Also, 97.07% and 85.85% % of Lecturers had normal fasting blood sugar (<5.6 mmol/L) and normal random blood sugar (<11.1mmol/dL) respectively. Again, 62% of lecturers eat breakfast and only 5.4% take fruits and vegetables of 5 servings per day/week. Moreover, 34.6% of lecturers are habitual late meal takers. Again, 30% of lecturers do not undertake any form of exercise. A Lecturer’s age was found to be statistically significant (p = 0.044). It was found that, lecturer aged over 50 years accounted for about 91.25%. Also, 18.54%, 16.10%, and 12.68% of lecturers in Health Sciences, Engineering, Agriculture, and Natural Resources had high blood pressure (systolic >130mmgH, diastolic >90mmgH). Lecturers from the Colleges of Health Science and Engineering were 4 times [COR: 4.11; 95% CI (1.30–12.95)] more likely to develop high blood pressure when compared to those in other Colleges. The prevalence of hypertension and diabetes among the university lecturers was found to be 54.6% and 17.07% respectively.ConclusionThe study concludes that modifiable lifestyles are precursors for the occurrence of hypertension and diabetes among the lecturers studied in a Ghanaian university.

The article theoretically substantiates and experimentally confirms the feasibility of using grass carp (Ctenopharyngodon Idella) for the production of healthy fish products. The relevance of the study is due to the need to use available sources of fish raw materials to manufacture products that meet modern market trends. The aim of the article is to confirm the nutritional benefits of grass carp, assess its potential for use in the development of healthy food products, and provide recommendations for the high-value use of freshwater fish resources. The article examines the consumer properties, chemical composition, biological value, and beneficial characteristics of grass carp as an ingredient for healthy eating. Based on an analysis of literature sources, it was found that grass carp is rich in protein of high biological value, has a low content of saturated fats, and contains polyunsaturated fatty acids (PUFA), vitamins, minerals. The results of organoleptic studies showed that grass carp has tender meat, few intermuscular bones, and a light fishy odor, demonstrating good technological adaptation and a high yield of edible parts. The results of the study of the chemical composition of the grass carp meat showed low fat content (0.96%), high protein content (19.64%), and high moisture content (79.38%). Studies of the amino acid composition have shown that the grass carp protein is not only complete, but also has an amino acid composition close to the ideal for the human body. The dominant amino acids are lysine, valine, and histidine. According to the results of the study of the mineral composition, it was found that the grass carp is a source of calcium, phosphorus, potassium, magnesium, selenium for the human body. A systematic analysis of the research results showed that regular consumption of grass carp helps reduce the risk of cardiovascular diseases, improves lipid metabolism, maintains normal body weight and blood sugar levels, and also has a positive effect on immune function, growth and development of children, and vision health in the elderly.

Reinforcement learning (RL) provides a flexible framework for optimizing personalized healthcare treatment options. In this work, we implemented a diabetes management problem using actor-critic reinforcement learning from a matrix game perspective, with the goal of maximizing long-term health outcomes. We simulated a diabetic patient over 20 weeks, with the actor recommending treatment plans (e.g., insulin and dietary interventions), while the critic determined the patient's benefit. The model was able to learn patient-specific plans that recommended treatment with normal blood sugar levels more frequently (75%) compared to using a fixed baseline (50%). We also developed a Python application to simulate diabetes models, providing visualizations of policy evolution, health outcomes, and value estimates. The results of this work demonstrate promising signs for integrating reinforcement learning into healthcare precision medicine, while highlighting future implementation challenges, addressing relevant safety constraints and appropriate data use.

Diabetes mellitus is one of the major scientific problems facing the human body due to its delayed detection at its onset. It is often referred to as the silent killer. Cardiovascular disease, renal disease, and neuropathy are among the many complications that can develop in people with diabetes. The pancreatic beta cells secrete the hormone insulin. Its regulation of metabolism is crucial for maintaining normal blood sugar levels. Due to the complications caused by chemotherapy and its high cost, medicinal herbs have been used in this field to reduce the effects of diabetes and lower blood levels. One such herb is basil. Its aqueous leaf extract is used to treat diabetes, given its content of active compounds such as flavonoids, alkaloids, saponins, and other compounds. Forty adult male albino rats were evaluated. They weighed between 200 and 250 grams and were 10 to 12 weeks old. Four groups were formed from them. A control group, which did not receive any therapy, was the first. On the other hand, 150 mg/kg bw of alloxan was administered to the second group. The third set of subjects received alloxan injections and a monthly dosage of 250 mg/kg bw of water-based basil leaves extract. The fourth set of subjects received a single alloxan injection, along with a monthly dosage of aqueous basil leaves extract (500 mg/kg body weight). Following the injection of alloxan, the findings demonstrated a significant decline (p &lt; 0.05) in the quantity of red blood cells, compressed cell volume, and hemoglobin concentration. As shown in the control group, the levels increased once more after administering the aqueous basil leaf extract. Nevertheless, in contrast to the control group, the alloxan injection resulted in a substantial increase (p&lt;0.05) in the number of white blood cells. It dropped down to levels similar to the control group after the administration of the water-based basil leaves extract (p&lt;0.05).

BackgroundKetoacidosis, a life‐threatening disease, can be caused by diabetes, alcohol consumption, and severe malnutrition, which are not uncommon in psychiatry. Diabetic and alcoholic ketoacidosis (AKA) are particularly frequent, and differentiating between these two is essential in terms of the substantially different treatment interventions. In recent years, the advent of sodium‐glucose co‐transporter 2 (SGLT2) inhibitors has made this distinction more difficult, mainly due to the increase in euglycemic diabetic ketoacidosis (euDKA)—ketoacidosis in normal or near‐normal blood sugar levels—in the presence of SGLT2 inhibitors.Case PresentationWe describe a 52‐year‐old man's case of severe euDKA caused by an SGLT2 inhibitor with alcohol use disorder. We initially suspected mainly AKA due to his mild disturbance of consciousness, habitual high intake of alcohol, and almost normal blood glucose level (142 mg/dL). We administered supplemental fluids and vitamin B complex instead of insulin, which is needed for euDKA, but his disorientation and metabolic acidosis worsened. Upon recognizing his inadequate response to fluid replacement and considering his history of SGLT2 inhibitor use, we identified euDKA as the primary pathology. Implementing continuous insulin therapy and tight glycemic control resulted in a rapid improvement in the patient's condition.ConclusionEven with normal or near‐normal blood sugar levels in a patient with suspected AKA, missing the possibility of euDKA can be fatal. Therefore, psychiatrists should remain vigilant for euDKA, especially in patients using SGLT2 inhibitors, by conducting frequent blood gas examinations.

Set it and forget it: Engineered cells for drug delivery.

This program is a response to significant public health challenges, where non-communicable diseases (NCDs) and poor oral health are still prevalent and impact the quality of life of residents, while awareness of routine health checkups and preventive behaviors remains low. This program aims to conduct early detection of NCD risk factors and increase community knowledge about healthy lifestyles and oral health maintenance. Activities include health examinations covering blood pressure, blood sugar, cholesterol, uric acid, and dental examinations, followed by health education and guidance sessions by the Regular Community Service Program (KKN) IPE 010 of Muhammadiyah University Yogyakarta. A total of 46 residents participated in the check-ups, with results showing a high prevalence of hypertension (63.1%) and high cholesterol (80%), while most participants had normal blood sugar levels (94.6%) and normal uric acid levels (80%). Dental examinations revealed a very high DMF-T index (7.91), indicating widespread and untreated dental problems. This program has proven to enhance community understanding regarding early detection, the importance of routine health monitoring, and the adoption of healthy lifestyle behaviors. These findings underscore the need for sustained promotive and preventive interventions through collaboration between healthcare workers and stakeholders to improve health outcomes.

Disclosure: M.D. Balina: None. J. Uy-Ho: None. H. Yao: None. S. Reburiano: None. E. David: None. F. Sorsona: None.Background: Autoimmune Polyglandular Syndromes (APS) are groups of 2 or more autoimmune endocrine and non-endocrine disorders that occur in discreet patterns. APS type 2, considered to be the most common form, is composed of primary adrenal insufficiency, type 1 diabetes mellitus, and autoimmune thyroid disease. Recognition of signs and symptoms of adrenal insufficiency before management of a diagnosed thyroid disease is of utmost importance. Case: This is a case of a 58-year-old female who was referred to endocrine service due to nodular goiter. Additional physical examination showed areas of hyperpigmentation, particularly sun-exposed areas, gums, and ears, which developed gradually over 3 years. On further probing, the patient reported brief episodes of lightheadedness, easy fatigability, and weight loss. She was initially diagnosed with hypothyroidism and tested positive for anti-thyroid peroxidase antibodies. She had mild hyponatremia with normal potassium and blood sugar levels. Further endocrine work-up showed low fasting 8 am serum cortisol levels at 1.99 mcg/dL associated with elevated ACTH of 1488 pg/mL. A tuberculosis infection was also ruled out. Ideally, 21-hydroxylase antibody testing should be done to diagnose autoimmune adrenal insufficiency. However, it was not available in our country. Anti-glutamic acid decarboxylase was negative. The adrenal CT scan result was also unremarkable. She was started on a physiologic dose of prednisone 5mg tab once daily and eventually given levothyroxine replacement for Hashimoto’s thyroiditis. On her latest follow-up, she reported significant improvement in daily activities and showed no signs of steroid excess. Conclusion: This case underscores the importance of the ability to diagnose clinical syndromes. Starting a patient on a thyroid hormone replacement for autoimmune thyroiditis without suspecting the presence of possible adrenal insufficiency will lead to worsening of the patient’s symptoms. Early recognition and intervention are crucial in preventing complications and improving the quality of life for affected individuals while also being able to anticipate the future development of associated disorders. Recommendation: Active screening for the development of type 1 diabetes mellitus in the future for our patient is recommended. Relatives should be advised of the early symptoms and signs of the principal component disease and should undergo screening every 3-5 years.Presentation: Monday, July 14, 2025

Metabolic syndrome is a collection of metabolic disorders that are closely related to an increased risk of developing diabetes mellitus and cardiovascular diseases in obese adolescents. Metabolic syndrome in adolescents is still reversible with varying timeframes. In adolescents with metabolic syndrome, the transition from homeostasis with normal blood sugar levels to type 2 diabetes occurs more rapidly compared to adults. Early diagnosis of metabolic syndrome is essential for prevention through lifestyle changes. Living in urban areas has a higher risk of obesity compared to living in rural areas after controlling for other variables. This activity aims to initially screen metabolic syndrome in middle school adolescents in urban and rural areas. The measurement of metabolic syndrome parameters involved 107 students, consisting of 47 middle school students in urban areas and 60 in rural areas. The measurement and data analysis results showed significant differences in body mass index (p&lt;0,001), waist size (p=0.03), waist circumference (p=0.007), and upper arm circumference (p=0.002) between students in urban and rural areas, while blood sugar levels, blood pressure, and neck circumference did not show significant differences.

The dietary habits and nutritional status of university students, particularly those in the field of biochemistry, are crucial for maintaining optimal health, academic performance, and professional success. This study aimed to assess the dietary habits and nutritional status of 400-level Biochemistry students at Federal University, Birnin Kebbi, Kebbi State. A total of 46 participants were assessed using structured questionnaires, anthropometric measurements, biochemical tests, and urinalysis. The majority of participants (54.3%) were aged 22–24 years, with 56.5% being female and 65.2% residing on-campus. Dietary habit analysis revealed that 43.5% consumed three meals daily, with breakfast being the most skipped meal (43.5%). Daily consumption of grains (87.0%) and proteins (76.1%) was high, while 54.3% consumed fruits daily. Nutritional assessments indicated that 43.5% rated their diets as average, and 65.2% believed their diets met their nutritional needs. Anthropometric analysis showed an average BMI of 20.50, placing most participants within the normal range, while biochemical tests revealed normal blood sugar (mean RBS = 6.99 mmol/L) and total protein levels (7.0–7.8 g/dL). Urinalysis showed no abnormalities, indicating overall good metabolic and nutritional health among the participants. These findings highlight healthy dietary practices among most students but underscore the need for enhanced nutrition education to improve dietary patterns further.

Hyperglycemia is a condition characterized by higher than normal blood sugar levels and is the main sign of diabetes mellitus. According to the International Diabetes Federation (IDF) in 2021, Indonesia ranks fifth with 19.47 million diabetics and a population of 179.72 million, and this makes the prevalence of diabetes in Indonesia 10.6%. One of the plants that is widely used as traditional medicine to reduce blood sugar levels is the brotowali plant (Tinospora crispa). This study aims to analyze the mechanism of active compounds of the brotowali plant (Tinospora crispa) that can inhibit the α-amylase enzyme through molecular tethering in silico. This study uses the method of tethering to receptors obtained from the PDB database (ID 1OSE). The positive control used was acarbose so as to obtain a new antihyperglycemic drug candidate with a good pharmacokinetic profile. The molecular tethering simulation results showed that the DL-Carnitine compound has the lowest ΔG value of -4.32 kcal/mol with a Ki value of 680.33 µM and a hydrophobic bond that plays an active role with the natural ligand ASP A:197. In terms of pharmacokinetics, the DL-carnitine compound is better than acarbose. It can be concluded that the DL-Carnitine compound is predicted to be carried out further as research as an antihyperglycemic candidate through α-amylase inhibition.

Primary polydipsia, or psychogenic polydipsia, is characterised by excessive fluid intake without an underlying physiological cause. If left untreated, primary polydipsia may result in life-threatening hyponatremia and can lead to complications such as nausea, vomiting, confusion and seizure episodes, potentially becoming life-threatening if not recognised and managed early. The aetiology of this condition is incompletely understood and is frequently attributed to psychiatric disorders, most commonly chronic schizophrenia. Psychogenic polydipsia occurs in up to 20% of psychiatric patients, and this case serves as a reminder to be cognisant of water overconsumption. This case report presents a 74-year-old male with a three-year history of excessive thirst and polyuria, which worsened following the death of his elder brother. Laboratory investigations showed normal random blood sugar and HbA1c levels, normal renal and liver function tests and mild hyponatremia, while radiological investigations were grossly normal. Ultimately, a water deprivation test ruled out diabetes insipidus, leading to a diagnosis of primary polydipsia. The patient was started on oral medication and psychotherapy after diagnosis, and he improved significantly with treatment. He was discharged on oral atypical antipsychotics, with water restriction and regular psychotherapy sessions for further improvement. This case highlights the importance of a thorough diagnostic evaluation of excessive thirst in elderly patients and the necessity of initiating appropriate treatment, which can prevent complications such as severe dilutional hyponatremia in this population.

Type 2 diabetes mellitus is a chronic metabolic disease that is common in the elderly and has a major impact on quality of life, especially through risk complications such as impaired vision, heart disease, kidney failure, and amputation. The elderly are often unaware of the early symptoms of diabetes because complaints are considered part of the aging process, such as fatigue or frequent urination. The Community Service Program (PKM) at Panti Werdha Hana aims to increase awareness of the elderly about the importance of early detection of diabetes through fasting blood sugar screening activities. This activity uses the Plan-Do-Check-Act (PDCA) approach which includes checking blood sugar levels with the Point-of-Care Testing (POCT) tool and educational counseling on risk factors and prevention of diabetes. The results showed that out of 76 participants, 8 people (10.53%) were identified as having diabetes, 9 people (11.84%) had prediabetes, and the rest had normal blood sugar levels. Early detection through screening has proven to be an important step in preventing diabetes progression and severe complications, as well as supporting the improvement of the quality of life of the elderly through timely interventions and ongoing education.

Community Service carried out by a group of students in order to advance the welfare of the community and educate the nation's life. This activity was carried out in Mersam Village, Mersam District, Batanghari Regency. It was carried out using the method of providing learning experiences in the form of socialization and counseling that went directly to the community carried out by students and lecturers, carried out within 30 days. The results of several activities that we have carried out are in the activity of checking hypertension and blood sugar health, the results were obtained from 40 people who participated in this activity, who had blood pressure&gt; 120/80 mmHg as many as 6 people, 120 - 129/80 - 84 mmHg as many as 9 people, 130 - 139/85 - 89 as many as 7 people, and&gt; 140/90 mmHg as many as 18 people. And have normal blood sugar levels (&gt;99 mg/dl) as many as 7 people, pre-diabetes (100-180 mg/dl) as many as 21 people, Diabetes (&gt;200 mg/dl) as many as 12 people. This KKN activity has succeeded in increasing the knowledge and participation of Mersam Village residents regarding personal and environmental health, increasing local roles and resources, increasing community independence and welfare as well as knowledge for Mersam Village residents regarding the correct use of drugs and safe cosmetics.

Background : Diabetes is a disease with metabolic disorders that make blood sugar levels above normal. Factors that can affect blood sugar levels are medication adherence, treatment adherence plays an important role in maintaining blood sugar levels in the normal range. Objective : To determine the relationship between medication adherence and blood sugar levels in patients with type 2 diabetes mellitus. Method : Quantitative with a cross sectional approach, a sample of 174 people was determined by random sampling. The research instrument used the Medication Adherence Scale-8 questionnaire as a measure of medication adherence and a glucometer as a measure of blood sugar levels. Test bivariate analysis using the Chi-Square test. Results : Of the 174 respondents, it was found that the majority of patients with moderate medication adherence were 82 people (46.9%). out of 174 respondents the majority of results with normal blood sugar levels were 88 people (50.3%) and with abnormal blood sugar levels were 87 people (49.7%). Statistical results obtained p value = 0.002 &lt; α (0.05) there is a relationship between adherence to treatment of blood sugar levels in patients with type 2 diabetes mellitus. Conclusion : There is a relationship between medication adherence and blood sugar levels in patients with type 2 diabetes mellitus in the working area of ​​the Pasundan Public Health Center, Samarinda Ulu.

Introduction and Objective: Glucokinase (GCK) maintains glucose homeostasis. Gck heterozygous inactivation mutation leads to maturity onset diabetes of the young 2 with clinical features of mild hyperglycemia and low insulin levels, and the incidence of microvascular complications is significantly low. ATP-Sensitive K+ Channel (K-ATP) is vital in regulating insulin secretion. Inactivated variants of Abcc8 encoding SUR1 subunit of the K-ATP can lead to hyperinsulinemia and kidney injury. Our study aims to elucidate the role of GCK and hyperinsulinemia in the development of kidney injury. Methods: Gck knockout mouse (Gck+/-) and Abcc8 R306C mutation mouse (Abcc8mut/+) were constructed using CRISPR-Cas9. After hybridization, we obtained Gck knockout and Abcc8 mutant heterozygote mouse (G&amp;A), observed metabolic phenotype and evaluated the kidney injury. Results: Gck +/- mice showed hyperglycemia and impaired glucose toleranc. Abcc8mut/+ mice showed normal blood sugar, elevated fasting insulin, and significant kidney injury. Compared to Abcc8mut/+ mice, G&amp;A mice developed elevated fasting blood glucose but lower insulin levels, and alleviated kidney injury. (Figure 1) Conclusion: Hyperinsulinemia leads to severe renal injury independent of hyperglycemia. Suppression of glucokinase on the basis of hyperinsulinemia can partially alleviate renal injury. Disclosure H. Lian: None. C. Yang: None. L. Ji: None. X. Han: None. Funding National Natural Science Foundation of China (82170814)