Non-glutinous Rice Research Articles

Effect of starch modification in the whole white rice grains on physicochemical properties of two contrasting rice varieties

The effect of acetylation of milled rice of selected rice varieties viz. TDK8 and DG on their physicochemical properties was investigated at different acetic anhydride concentrations (1–7 g per 100 g of milled rice samples in 225 mL of water). Results showed that the intact starch of milled grains of both selected verities could be acetylated (Acetyl % for TDK8 = 2.18 and for DG = 0.89) even with 1 g of acetic anhydride. X-ray diffraction patterns showed that acetylation resulted in reduced crystallinity. Acetylation resulted in reduced peak and final viscosities and gel strength, particularly in glutinous (TDK 8) and non-glutinous (DG) rice. Thermal study showed acetylation resulted in reduced thermal transition temperatures and enthalpy of both varieties. Although the increase in retrogradation thermal temperatures was observed, the amount of retrograded starch was decreased in both varieties. Furthermore, the texture of cooked acetylated grains was less hard and more adhesive. In vitro digestion showed significant decrease in GI possibly due to structural changes in the native starch during acetylation. These findings suggest a good potential of applying acetic anhydride pre-treatments in rice processing, especially glutinous varieties to control the hardness and maintain the stickiness properties of rice.

Characteristics of Rice Flour Breads Using Yeast Isolated from Pear Red Bartlett Fruits

To develop high qualities of rice flour breads, we tried to prepare breads using rice flours from major five non-glutinous rice cultivars on market shares of Japan and wild-type Saccharomyces cerevisiae strain YTPR1 isolated from pear Red Bartlett fruits. Apparent amylose contents of rice flours were measured about 12.1-19.9%. Damaged starch contents of these flours were about 22% regardless to the kinds of rice cultivars. Gluten was added about 20 wt.% based on rice flour, and breads were made in the same way. Any bread has caused caving. Next, the amount of water added in dough was regulated in consideration of moisture contents of rice flours. Except for Akitakomachi flour, breads largely expanded, although loaves volumes did not amount to that on bread made from bread flour and commercially available baker’s dried yeast. It was observed correlation between the amount of water and amylose contents of rice flours with R2 = 0.703. It suggested that the amount of water added in dough might estimate from amylose contents of rice flours. Specific volumes of these loaves were low compared with that made from bread flour. However, by sensory analysis, breads made from Hinohikari and Haenuki flours had total points closest to that made from bread flour: it could produce high quality of breads using Hinohikari and Haenuki flours and yeast isolated from pear Red Bartlett fruits.

Development and characterization of a new rice cultivar, 'Chikushi-kona 85', derived from a starch-branching enzyme IIb-deficient mutant line.

A new super-hard rice cultivar, ‘Chikushi-kona 85’, which was derived from a cross between ‘Fukei 2032’ and ‘EM129’, was developed via bulk method breeding. ‘Chikushi-kona 85’ showed a higher content of resistant starch than the normal non-glutinous rice cultivar, ‘Nishihomare’, and a higher grain yield than the first super-hard rice cultivar, ‘EM10’. The amylopectin chain length of ‘Chikushi-kona 85’ and its progenitor line ‘EM129’ was longer than that of ‘Nishihomare’, and was similar to that of ‘EM10’. This suggests that the starch property of ‘Chikushi-kona 85’ was inherited from ‘EM129’, which is a mutant line deficient in a starch branching enzyme similar to ‘EM10’. Genetic analysis of ‘Chikushi-kona 85’ crossed with ‘Nishihomare’ also showed that the starch property of ‘Chikushi-kona 85’ was regulated by a single recessive gene. Consumption of processed cookies made from ‘Chikushi-kona 85’ flour showed a distinctive effect in controlling blood sugar levels in comparison to the normal non-glutinous rice cultivar ‘Hinohikari’. These results show that ‘Chikushi-kona 85’ is a novel genetic source to develop new products made of rice, which could reduce calorie intake and contribute to additional health benefits.

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A new super-hard rice cultivar, ‘Chikushi-kona 85’ (center right), showed higher content of resistant starch than the normal non-glutinous rice cultivar, ‘Nishihomare’ (center left). Grain appearance of ‘Chikushi-kona 85’ (lower right) was opaque, which differed from the translucent appearance of normal non-glutinous rice cultivar, ‘Nishihomare’ (lower left). Consumption of processed cookies made of Chikushi-kona 85 flour (lower center) showed distinctive effect in controlling blood sugar levels. Thus, ‘Chikushi-kona 85’ may be a novel genetic source to develop new products made of rice, which may reduce calorie intake and provide additional health benefits (This issue, p. 278–283).

粳米から製造する千葉県伝統食品「性学もち」の水分含量と測定時温度が物性値に与える影響

粳米から製造する千葉県伝統食品「性学もち」の水分含量と測定時温度が物性値に与える影響

Changes in pasting properties and free fatty acids of different brown rice cultivar during storage

Paddy rice is typically stored during postharvest until rice grain is processed into brown rice and milled rice by hulling and milling procedure, respectively. Recently, instead of storing paddy rice, storage of brown rice has been in the spotlight because it is more convenient and economically feasible. Different brown rice cultivars with varying amylose contents including waxy rice, medium-waxy rice, and non-glutinous rice were stored in room temperature storage for four months, and the changes in grain qualities of brown rice were evaluated. Amylose content significantly affected pasting properties in which rice cultivar with higher amylose content showed longer pasting time and higher peak viscosity. Storage also affected pasting viscosities, showing an increase in peak viscosity, but a decrease in breakdown viscosity. The changes in pasting viscosity during storage could be an important starch property for aged brown rice utilization. Waxy brown rice showed the weakest aging property in terms of free fatty acids (FFA) accumulation, whereas non-glutinous rice was more substantial grain quality against aging. The FFA values of two months storage were not significantly different from the initial FFA contents, suggesting that brown rice stored in room temperature for two months could be feasible for direct consumption of brown rice.

Characteristics of Korean Alcoholic Beverages Produced by Using Rice Nuruks Containing Aspergillus oryzae N159-1

Herein, nuruks derived from non-glutinous and glutinous rice inoculated with Aspergillus oryzae N159-1 (having high alpha-amylase and beta-glucosidase activities) were used to produce Korean alcoholic beverages. The resultant beverages had enhanced fruity (ethyl caproate and isoamyl alcohol) and rose (2-phenethyl acetate and phenethyl alcohol) flavors and high taste scores.

紫黒米(黒米)モチ品種「きたのむらさき」と新育成ウルチ品種「TMK^*」の育成

紫黒米(黒米)モチ品種「きたのむらさき」と新育成ウルチ品種「TMK^*」の育成

紫黒米(黒米)新育成ウルチ品種「TMK^*」の発芽について

紫黒米(黒米)新育成ウルチ品種「TMK^*」の発芽について

Qualitative and quantitative characterization of secondary metabolites and carbohydrates in Bai-Hu-Tang using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and ultraperformance liquid chromatography coupled with photodiode array detector

Bai-Hu-Tang (BHT), a classic traditional Chinese medicine (TCM) formula used for clearing heat and promoting body fluid, consists of four traditional Chinese medicines, i.e., Gypsum Fibrosum (Shigao), Anemarrhenae Rhizoma (Zhimu), Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (Zhigancao), and nonglutinous rice (Jingmi). The chemical composition of BHT still remains largely elusive thus far. To qualitatively and quantitatively characterize secondary metabolites and carbohydrates in BHT, here a combination of analytical approaches using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and ultraperformance liquid chromatography coupled with photodiode array detector was developed and validated. A total of 42 secondary metabolites in BHT were tentatively or definitely identified, of which 10 major chemicals were quantified by the extracting ion mode of quadrupole time-of-flight mass spectrometry. Meanwhile, polysaccharides, oligosaccharides, and monosaccharides in BHT were also characterized via sample pretreatment followed by sugar composition analysis. The quantitative results indicated that the determined chemicals accounted for 35.76% of the total extract of BHT, which demonstrated that the study could be instrumental in chemical dissection and quality control of BHT. The research deliverables not only laid the root for further chemical and biological evaluation of BHT, but also provided a comprehensive analytical strategy for chemical characterization of secondary metabolites and carbohydrates in traditional Chinese medicine formulas.

Development of an HPLC-UV Method for Free Asparagine in Grains

A novel analytical method was developed for the determination of free asparagine (Asn), which is a precursor of acrylamide, in grains. Asn was extracted from a sample with 5% (w/v) aqueous trichloroacetic acid solution, and the crude extract was cleaned up using a reversed-phase solid-phase cartridge. The cleaned extract was derivatized with dansyl chloride and analyzed by HPLC-UV. HPLC separation was performed by gradient elution on a ODS column using 0.01 mol/L ammonium acetate and acetonitrile mixture as the mobile phase. The calibration curve was linear in the range of 0.5-100 μg/mL. The mean recoveries from potato starch, non-glutinous rice flour and whole wheat flour ranged from 97.7 to 102.6%, the repeatability (RSDs) ranged from 0.8 to 2.0%, and the within-laboratory reproducibility (RSDwr) ranged from 1.4 to 6.2%. Limits of quantitation (LOQs) were 13 mg/kg for potato starch and 4 mg/kg for non-glutinous rice flour. The quantitative values obtained for about 15 kinds of grains using this developed method were approximately equal to those obtained with an automatic amino acid analyzer.

고령자의 선호 식재 및 고령친화식품의 인지, 경험에 대한 분석

Development of elderly foods that improve health among the older population is needed. The purpose of this study was to investigate the preference of specific foods for development as elderly foods. A one-to-one survey method was used for data collection from 150 elderly people attending senior welfare centers. The results of the study showed that the preferred cooking materials were vegetables, meats and fishes; and preferred cooking method was soup and stew rather than deep-frying. The elderly preferred meats and seafood but they did not eat them frequently. The results of investigating the preference of specific foods including meats, Kimchi, nuts and rice cakes indicated that old people liked broiled meats, Chinese cabbage Kimchi, walnuts and nonglutinous rice cakes the most, and they did not feel comfortable with chewing the foods. Most respondents were not familiar with the elderly foods. Moreover, the most important factors affecting their choice of elderly foods were hygiene, nutrition and flavor.

Multiresponse optimization of a UPLC method for the simultaneous determination of tryptophan and 15 tryptophan-derived compounds using a Box-Behnken design with a desirability function.

A Box-Behnken design was used in conjunction with multiresponse optimization based on the desirability function to carry out the simultaneous separation of tryptophan and 15 derivatives by Ultra Performance Liquid Chromatography. The gradient composition of the mobile phase and the flow rate were optimized with respect to the resolution of severely overlapping chromatographic peaks and the total run time. Two different stationary phases were evaluated (hybrid silica and a solid-core-based C18 column). The methods were validated and a suitable sensitivity was found for all compounds in the concentration range 1-100μgL-1 (R2>0.999). High levels of repeatability and intermediate precision (CV less than 0.25% and 1.7% on average for the retention time and the signal area, respectively) were obtained. The new method was applied to the determination tryptophan and its derivatives in black pigmented glutinous and non-glutinous rice grain samples.

Influence of ethnic traditional cultures on genetic diversity of rice landraces under on-farm conservation in southwest China.

BackgroundCrop genetic resources are important components of biodiversity. However, with the large-scale promotion of mono-cropping, genetic diversity has largely been lost. Ex-situ conservation approaches were widely used to protect traditional crop varieties worldwide. However, this method fails to maintain the dynamic evolutionary processes of crop genetic resources in their original habitats, leading to genetic diversity reduction and even loss of the capacity of resistance to new diseases and pests. Therefore, on-farm conservation has been considered a crucial complement to ex-situ conservation. This study aimed at clarifying the genetic diversity differences between ex-situ conservation and on-farm conservation and to exploring the influence of traditional cultures on genetic diversity of rice landraces under on-farm conservation.MethodsThe conservation status of rice landrace varieties, including Indica and Japonica, non-glutinous rice (Oryza sativa) and glutinous rice (Oryza sativa var. glutinosa Matsum), was obtained through ethno-biology investigation method in 12 villages of ethnic groups from Guizhou, Yunnan and Guangxi provinces of China. The genetic diversity between 24 pairs of the same rice landraces from different times were compared using simple sequence repeat (SSR) molecular markers technology. The landrace paris studied were collected in 1980 and maintained ex-situ, while 2014 samples were collected on-farm in southwest of China.ResultsThe results showed that many varieties of rice landraces have been preserved on-farm by local farmers for hundreds or thousands of years. The number of alleles (Na), effective number of alleles (Ne), Nei genetic diversity index (He) and Shannon information index (I) of rice landraces were significantly higher by 12.3–30.4 % under on-farm conservation than under ex-situ conservation. Compared with the ex-situ conservation approach, rice landraces under on-farm conservation programs had more alleles and higher genetic diversity. In every site we investigated, ethnic traditional cultures play a positive influence on rice landrace variety diversity and genetic diversity.ConclusionMost China’s rice landraces were conserved in the ethnic areas of southwest China. On-farm conservation can effectively promote the allelic variation and increase the genetic diversity of rice landraces over the past 35 years. Moreover, ethnic traditional culture practices are a crucial foundation to increase genetic diversity of rice landraces and implement on-farm conservation.Electronic supplementary materialThe online version of this article (doi:10.1186/s13002-016-0120-0) contains supplementary material, which is available to authorized users.

저온 적응성 효모와 발효온도에 따른 약주의 품질특성 변화

The objective of this study was to evaluate the effects of low temperature-adapted Saccharomyces cerevisiae Y297 and fermentation temperatures on the quality of Yakju brewed. Physicochemical properties of Yakju brewed were compared pH, total acidity, ethanol, free amino acid, organic acid contents, and volatile flavor compounds in S. cerevisiae Y297 with control treatment. Cooked non-glutinous rice and saccharogenic amylase in koji were mixed with ethanol-producing yeasts and then fermented at 15℃, 20℃, and 25℃ for 20 days. Yakju brewed using the Y297 treatment showed the highest ethanol yield (17.9%) at 20℃. Expression of heat shock protein (HSP) 104 was evaluated by immunoblotting as an indication of adaptation to low temperatures (15℃); levels of the HSP104 protein were higher in the Y297 treatment than in the control. Organic acid analysis showed that the lactic acid content of Yakju brewed using the control was the highest at 25℃. Finally, free amino acid analysis showed that the Y297 treatment had a higher proportion of essential amino acids than the control. Overall, these results indicate that S. cerevisiae Y297 could be used as a suitable yeast for Yakju brewed under low temperature (15℃) condition.

Antioxidative flavonoids in adzuki-meshi (rice boiled with adzuki bean) react with nitrite under simulated stomach conditions

Non-glutinous rice boiled with adzuki bean (adzuki-meshi) is often prepared in Japan, and the colour of rice in adzuki-meshi is purplish red, suggesting the transfer of adzuki bean components to rice. In fact, red-coloured rice of adzuki-meshi contained adzuki bean flavonoids such as (+)-catechin, (−)-epicatechin, taxifolin, quercetin 7-O-glucoside, rutin, quercetin, and cyanidin-catechin conjugates (vignacyanidins). A salivary component nitrite reacted with the above flavonoids in adzuki-meshi under the conditions simulating the stomach. The reaction resulted in the decrease in the antioxidative components of adzuki-meshi, and the decrease was accompanied by the production of functional compounds like nitric oxide (•NO), 6,8-dinitrosocatechin, 6,8-dinitrosoepicatechin, and 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone from nitrous acid, (+)-catechin, (−)-epicatechin, and quercetin, respectively. In this manuscript, functions of adzuki-meshi are discussed considering the reactions of the flavonoids with nitrite in the stomach.

Genome-wide association study of eating and cooking qualities in different subpopulations of rice (Oryza sativa L.).

BackgroundStarch and protein are two major components of polished rice, and the amylose and protein contents affect eating and cooking qualities (ECQs). In the present study, genome-wide association study with high-quality re-sequencing data was performed for 10 ECQs in a panel of 227 non-glutinous rice accessions and four derived panels.ResultsPopulation structure accounted for high phenotypic variation in three routine panels and had minor effects on subspecies-based panels. Using the mixed linear model method based on the P + K model, we detected 29, 24, 16, 17, and 29 loci that were significant for ECQ parameters in each of the five panels. Some of these loci were close to starch synthesis-related genes. Two quantitative trait loci (QTLs) (chr.9: 15417525 ~ 15474876; 17538294 ~ 18443016) for several starch paste viscosity properties detected in four panels were close to the isoamylase 3 gene, one QTL (chr.1: 30627943 ~ 31668474) for consistency detected in three panels was close to the starch synthase IV-1 gene. The QTL (chr.7: 1118122 ~ 1967247) for breakdown (BD), detected in the whole panel and japonica panel, and one QTL (chr.7: 25312126 ~ 26540950) for BD and setback (SB), detected in the whole panel and indica panel, may be specific gene alleles in japonica or indica panels. One previously detected QTL (chr.11: 22240707 ~ 22563596) for protein content and one new QTL (chr.5: 7756614 ~ 8042699) for many ECQ traits detected in more than two panels, may represent valuable targets for future cloning of the underlying genes.ConclusionsThis study detected minor-effect QTLs affecting ECQs, and may increase our understanding of the genetic differences regulating the formation of ECQ between indica and japonica varieties.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3000-z) contains supplementary material, which is available to authorized users.

Effect of steaming, freezing, and re-steaming on the texture properties of non-glutinous rice cakes

The effects of steaming time (6, 8, and 10 min), freezing storage period, and re-steaming for thawing on the textural properties of non-glutinous rice cakes (baekseolgi) were investigated. As the steaming time increased, the rice cakes softened. In particular, the sample that was steamed for 10 min showed lower hardness than those steamed for shorter periods. A short period of steaming was insufficient for water bound to the surface of the starch granules to penetrate the granules in the dough. During the re-steaming process of the frozen non-glutinous rice cake samples, the retrogradation of starch and water syneresis contributed to the increased hardness of non-glutinous rice cakes.

Retrogradation—Digestibility Relationship of Selected Glutinous and Non-Glutinous Fresh and Stale Cooked Rice

This study examined the retrogradation and digestibility relationship of fresh and stale cooked rice of three rice varieties: glutinous (TDK11) and non-glutinous (Doongara and floating rice). The effect of rice variety, degree of milling and retrogradation (staling) of cooked rice on the estimated glycaemic index was determined. Although high-glycaemic index values were obtained for fresh cooked rice of all varieties, staling rice at 4oC for 24 h showed positive effect on floating rice only, yielding intermediate-glycaemic index. The effect of staling on retrogradation rates was corroborated by changes in x-ray diffraction peaks. The thermal and textural properties of rice samples showed higher pasting temperature, final viscosity, and hardness, and lower peak viscosity and adhesiveness for fresh cooked non-glutinous varieties, which were also significantly affected by degree of milling, in terms of hardness, after retrogradation.

Variability in amylose content of Bangladeshi rice cultivars due to unique SNPs in Waxy allele

Waxy gene (Granule Bound Starch Synthase I) is responsible for amylose synthesis in the rice endosperm. Several mutations in this gene have been shown to be responsible for variable amylose content (AC) phenotypes. The G/T mutation in 5′ splice site of Waxy intron 1 has been traced as the origin of the glutinous rice phenotype and differentiates low AC from non-glutinous intermediate/high AC rice. Sequencing of Waxy promoter and 5′ noncoding regions from 22 rice cultivars showed that the evolutionary pattern of all Bangladeshi non-glutinous and most glutinous rice accessions are in line with the general patterns of South and Southeast Asia. However, three cultivars Khara Beruin, Modhu Beruin White and Kathali Beruin Red with low to very low amylose lacked the G/T splice site mutation. These were more closely related to non-glutinous cultivars based on their SNP patterns in promoter and noncoding regions. Further sequencing revealed a unique C deletion at a pyrimidine tract of intron 5 of these three cultivars that may cause slippage of intron splicing. Additional SNPs at intron 9 and 10 were also identified among these cultivars. These Bangladeshi-genotype-specific mutations could be the cause of waxy or low amylose phenotypes in these glutinous accessions.