Reduce N2O Emissions Research Articles

Contribution of zeolite to nitrogen retention in chicken manure and straw compost: Reduction of NH3 and N2O emissions and increase of nitrate

Co-composting of chicken manure, straw and zeolite was investigated in a water bath heating system to estimate the effect of zeolite on physicochemical properties and metabolic functions related to nitrogen conversion. The results indicated that NH3 catches by zeolite was concentrated in the early stage and zeolite with 10 % addition reduced 28 % NH3 and 55 % N2O emissions as compost ended. The nitrate content in 10 % zeolite group was 17 % higher than that in control group. There was no significant increase of NO2− in zeolite group. More NO2− formed NH3, rather than being converted to NOx through denitrification. The abundance of nitrification genes amoA and hao increased except nxrA in zeolite groups. Denitrification was the most obvious at 20 d and zeolite decreased the abundance of denitrification genes narG, nirK and nosZ at this time.

The Effect of Biochar and Straw Return on N2O Emissions and Crop Yield: A Three-Year Field Experiment

To evaluate the effects of application of biochar and straw return for consecutive years on N2O emissions and crop yields in North China, a three-year field experiment of applying biochar and straw following a ten-year application was conducted in a wheat–maize rotation system. Four treatments were set up, including F (NPK fertilizer only); FB (NPK fertilizer + 9.0 t·ha−1 biochar); FS (NPK fertilizer + straw); and FSB ((NPK fertilizer + 9.0 t·ha−1 biochar combined with straw). The results showed that compared with the F treatment, the FB treatment significantly reduced soil N2O emissions by 20.2%, while the FS and FSB treatments increased it by 23.7% and 41.4%, respectively. The FB treatment reduced soil N2O emissions by 15.1% in the wheat season and 23.2% in the maize season, respectively. The FS and FSB treatments increased the N2O emissions by 20.7% and 36.7% in the wheat season, respectively, and by 25.5% and 44.2% in the maize season, respectively. In the wheat season, the soil water content (SWC), NO3−-N content and pH were the main influencing factors of the soil N2O emissions. In the maize season, SWC and NO3−-N content were the main influencing factors. In addition, the FB, FS and FSB treatments increased the crop yield by 4.99%, 8.40% and 10.25% compared with the F treatment, respectively. In conclusion, consecutive application of biochar can significantly reduce N2O emissions and improve crop yield. Although FS and FSB treatments can also improve the crop yield, they are not beneficial to suppressing N2O emissions. Therefore, the successive application of biochar is an effective measure to reduce N2O emissions and maintain crop yield.

Enhanced efficiency urea fertilizers and timing effects on N2O emissions from spring wheat production in Manitoba

AbstractOpportunities exist to reduce nitrous oxide (N2O) emissions from nitrogen (N) fertilizers using enhanced efficiency fertilizers (EEFs) and managing application timing. This study examined (1) application timing (fall/spring) and (2) fertilizer N source on N2O emissions, yield, and N uptake of Canadian hard red spring wheat (Triticum aestivum L.) in Southern Manitoba. Fertilizer N sources included granular urea and four EEF products: (1) polymer‐coated urea (environmentally smart nitrogen [ESN]); (2) urea plus nitrification inhibitor (eNtrench); (3) urea plus urease inhibitor (Limus); and (4) urea plus nitrification and urease inhibitor (SuperU). Nitrification‐inhibited products most consistently reduced N2O emissions while maintaining productivity. Compared to urea alone, urea + eNtrench was most effective in reducing cumulative N2O emissions by 47%–64% at four of six site‐years. SuperU reduced N2O emissions by 37%–57% at three of six site‐years. ESN and urea + Limus did not affect emissions in most years. Wheat yield, protein, and N uptake were unaffected by N source in five of six site‐years. Compared to spring, fall application gave greater N2O emissions by 33%–67% at three of six site‐years due to spring‐thaw emissions. Fall was inferior to spring application in wetter site years with lower yield, protein, and N uptake. Overall, nitrification‐inhibited products—either alone or with a urease inhibitor—are a promising tool to reduce N2O emissions while maintaining wheat productivity in Manitoba. However, given that there were few consistent increases in yield or protein, the additional cost of the inhibitors will be a barrier to adoption.

Inclusion of sorghum grain rich in condensed tannins in the diet of steers did not affect the nitrogen utilization efficiency but increased the urine nitrous oxide emissions1

The objectives of this study which included two experiments were to investigate the effects of dietary inclusion of sorghum grain rich in condensed tannins (CT) [18.9 g kg-1 dry matter (DM)] on nitrogen (N) metabolism and urine nitrous oxide (N2O) emissions of beef steers. In experiment 1, six Limousin × Luxi steers with an initial body weight of 245 ± 18.70 kg liveweight were used as the experimental animals. Three levels of sorghum grain, i.e. 0, 167 and 338 g kg-1 DM were included in diets as experimental treatments. The animals and the treatments were randomly assigned to a replicated 3 × 3 Latin square design. In experiment 2, a static incubation technique was used to determine the N2O emissions of the urine samples collected in experiment 1. The results of experiment 1 showed that dietary inclusion of sorghum grain linearly increased the faecal N excretion (P = 0.001), the total N excretion (P = 0.010) and the faecal N to N intake ratio (P = 0.021), but did not affect the N retention and the N utilization efficiency (P > 0.10). The plasma metabolomic data showed that dietary inclusion of sorghum grain increased the relative concentrations of phenolic acid (N1,N5,N10-tris-trans-p-coumaroylspermine and prenyl cis-caffeate) and carnitine (3-hydroxyisovalerylcarnitine and linoelaidyl carnitine). The results also showed that dietary inclusion of sorghum grain linearly increased the urinary urea excretion (P = 0.010) and decreased the urinary excretion of purine derivatives (P = 0.041) as well as the estimated rumen microbial N flow (P = 0.012) based on urinary purine derivatives. The results of experiment 2 showed that including sorghum grain in the diet linearly increased the average concentrations of NH4+-N (P = 0.012), NO2--N (P = 0.009), NO3--N (P = 0.001) and the total inorganic N (P < 0.001) in the soil enriched with urine samples. The urine sample N2O-N flux (P = 0.001), the estimated steer urine N2O-N flux (P = 0.021) and the N2O-N to urinary N ratio (P = 0.038) linearly increased with increasing inclusion of sorghum grain in the diet. In conclusion, dietary inclusion of sorghum grain containing high CT at 167 and 338 g kg-1 DM did not affect the N retention rate of steers but increased the urine N2O-N emissions by 5.7% and 31.4%, respectively. For reducing N2O emissions to the environment, high levels of sorghum grain are not suggested to be included in the ration of steers.

Biochar mitigates N2O emissions by promoting complete denitrification in acidic and alkaline paddy soils

AbstractBiochar is an efficacious amendment for mitigating nitrous oxide (N2O) emissions in soils. Nevertheless, the underlying mechanisms responsible for reduced N2O emissions by biochar in paddy soils remain inadequately elucidated. Here, using two typical paddy soils with contrasting pH values (5.40 and 7.56), the N2 and N2O fluxes and the associated functional genes were investigated in soil amended with varying amounts of biochar (0%, 0.5%, and 5%, weight/weight) via soil slurry incubation integrated with the N2/Ar technique and qPCR analysis. The results showed that N2O fluxes were significantly (p &lt; 0.05) reduced by 0.65–3.64 times following biochar amendment, concomitant with a significant (p &lt; 0.05) increase in N2 fluxes (5.47–46.14%) in both acidic and alkaline paddy soils. As a result, the N2O/(N2O + N2) ratios were significantly (p &lt; 0.05) reduced by 1.53–4.65 fold in both soil types. In acidic paddy soils, the enhanced denitrification rates and the decreased N2O/(N2O + N2) ratios exhibited a strong correlation with increased pH values. In alkaline paddy soil, these changes were ascribed to the enhanced nosZ Clade I gene abundance and nosZ/(nirS + nirK) ratio. Our findings reveal that biochar primarily mitigates N2O emissions in paddy soils by promoting its reduction to N2.

Spent mushroom substrate as a substitute for chemical fertilizer changes N-cycling genes and reduces N2O emission in different textured soils

Spent mushroom substrate as a substitute for chemical fertilizer changes N-cycling genes and reduces N2O emission in different textured soils

Nitrogen addition stimulates N2O emissions via changes in denitrification community composition in a subtropical nitrogen-rich forest

Microbially driven nitrification and denitrification play important roles in regulating soil N availability and N2O emissions. However, how the composition of nitrifying and denitrifying prokaryotic communities respond to long-term N additions and regulate soil N2O emissions in subtropical forests remains unclear. Seven years of field experiment which included three N treatments (+0, +50, +150 kg N ha−1 yr−1; CK, LN, HN) was conducted in a subtropical forest. Soil available nutrients, N2O emissions, net N mineralization, denitrification potential and enzyme activities, and the composition and diversity of nitrifying and denitrifying communities were measured. Soil N2O emissions from the LN and HN treatments increased by 42.37% and 243.32%, respectively, as compared to the CK. Nitrogen addition significantly inhibited nitrification (N mineralization) and significantly increased denitrification potentials and enzymes. Nitrification and denitrification abundances (except nirK) were significantly lower in the HN, than CK treatment and were not significantly correlated with N2O emissions. Nitrogen addition significantly increased nirK abundance while maintaining the positive effects of denitrification and N2O emissions to N deposition, challenging the conventional wisdom that long-term N addition reduces N2O emissions by inhibiting microbial growth. Structural equation modeling showed that the composition, diversity, and abundance of nirS- and nirK-type denitrifying prokaryotic communities had direct effects on N2O emissions. Mechanistic investigations have revealed that denitrifier keystone taxa transitioned from N2O-reducing (complete denitrification) to N2O-producing (incomplete denitrification) with increasing N addition, increasing structural complexity and diversity of the denitrifier co-occurrence network. These results significantly advance current understanding of the relationship between denitrifying community composition and N2O emissions, and highlight the importance of incorporating denitrifying community dynamics and soil environmental factors together in models to accurately predict key ecosystem processes under global change.

Effects of extra-extreme precipitation variability on multi-year cumulative nitrous oxide emission in a semiarid grassland

High temporal precipitation variability, characterized by less frequent but larger-magnitude precipitation events, is increasing. However, how precipitation variability affects N2O emissions and the underlying mechanisms remain unclear, especially at multiple-year scales. We conducted a 3-year manipulative experiment in which the same long-term mean growing season precipitation total was repackaged into events of inversely varying magnitude and frequency to simulate four levels of precipitation variability (extra-extreme, extreme, medium, and normal) in a semiarid grassland. Cumulative N2O emissions was the smallest under the extra-extreme precipitation variability scenario (6 very large rainfall events), 26% less than emission under the other three treatments (10, 16, and 24 rainfall events) over the three growing seasons. However, this difference was almost entirely due to three sampling events in the third year. Plant community (biomass and biodiversity), soil abiotic properties (water, dissolved organic carbon and pH), soil microbial biomass (carbon, nitrogen and the ratio), and soil functional genes (archaeal and bacterial amoA, nirS, nirK, narG, and nosZ) explained 61% of the variation in N2O emissions in response to the precipitation variability. Structural equation modelling indicated that the precipitation variability had direct positive effects, and indirect negative effects via soil abiotic properties, on soil functional genes that ultimately had positive effects on N2O emissions. The reduction in N2O emission in the extra-extreme precipitation variability scenario in the third year was due to low levels of soil water content, soil pH, aboveground biomass, and microbial biomass carbon simultaneously. Our results suggest that at the multi-year timescale, semiarid grasslands may have negative feedbacks to future precipitation regimes with higher variability.

Estimating Yangtze River basin's riverine N2O emissions through hybrid modeling of land-river-atmosphere nitrogen flows

Riverine ecosystems are a significant source of nitrous oxide (N2O) worldwide, but how they respond to human and natural changes remains unknown. In this study, we developed a compound model chain that integrates mechanism-based modeling and machine learning to understand N2O transfer patterns within land, rivers, and the atmosphere. The findings reveal a decrease in N2O emissions in the Yangtze River basin from 4.7 Gg yr−1 in 2000 to 2.8 Gg yr−1 in 2019, with riverine emissions accounting for 0.28% of anthropogenic nitrogen discharges from land. This unexpected reduction is primarily attributed to improved water quality from human-driven nitrogen control, while natural factors contributed to a 0.23 Gg yr−1 increase. Notably, urban rivers exhibited a more rapid N2O efflux (FN2O), with upstream levels nearly 3.1 times higher than rural areas. We also observed nonlinear increases in FN2O with nitrogen discharge intensity, with urban areas showing a gradual and broader range of increase compared to rural areas, which exhibited a sharper but narrower increase. These nonlinearities imply that nitrogen control measures in urban areas lead to stable reductions in N2O emissions, while rural areas require innovative nitrogen source management solutions for greater benefits. Our assessment offers fresh insights into interpreting riverine N2O emissions and the potential for driving regionally differentiated emission reductions.

Microbiome and transcriptome analyses reveal the influence of calcined dolomite application on Eriocheir sinensis in a rice–crab co-culture system

Co-culture systems of rice and aquatic animals can contribute to the ecological intensification of agriculture by reducing nutrient loss and the need for N fertilizer application and by enhancing nutrient-use efficiency. However, the input of high-protein diets into paddy fields, to facilitate the growth of aquatic animals, has been found to increase N pollution and acidification of the soil. Although soil amendments have been widely used to ameliorate acidic soils, reduce N2O emissions, and improve agronomic production, the relationship between soil amendments and aquatic animal remains unclear. Therefore, this study investigated the effects of calcined dolomite (hereafter referred to as dolomite) as an acidic soil amendment and Ca–Mg supplement in rice–crab co-culture using Eriocheir sinensis crabs (Chinese mitten crabs). High-throughput sequencing was used to examine crab bacterial community composition and crab hepatopancreas biology. Although the water pH was significantly increased in the dolomite group, the number, composition, and diversity of bacteria identified in crab gut microbiome did not vary significantly between the dolomite and control groups. In the dolomite group, the probiotic agents Candidatus Hepatoplasma and Lactobacillus were highly abundant in the crab gut, and immune- and retinol metabolism-related genes were significantly upregulated in the crab hepatopancreas. Overall, dolomite application increased crab health and water pH. Dolomite is a low-cost amendment, with better stability, compared to other soil amendments, thus making it ideal for sustainable and clean rice–aquatic animal co-culture.

Microbial mechanism of biochar addition to reduce N2O emissions from soilless substrate systems

The soilless peat-based substrate partially solves the global soil problem in greenhouse vegetable production. However, it still produces serious N2O emissions due to the application of nutrient solutions. The pyrolysis biochar is regarded as an effective measure to reduce soil N2O emissions. However, the effect and mechanism of biochar on N2O emissions from the soilless substrate remain unknown. Therefore, this study set up six treatments by adjusting the ratio of biochar addition of peat-based substrate: 0% (0BC), 2% (2BC), 4% (4BC), 6% (6BC), 8% (8BC) and 10% (10BC) (v/v). The results showed that compared to the control treatment, N2O emissions reduced by 81%, 71%, 51%, 61%, and 75% in the 2BC, 4BC, 6BC, 8BC and 10BC treatments, respectively. In addition, lettuce yield increased by 10% and 7% in the 2BC and 4BC treatments and decreased by 0.5%, 4% and 6% in the 6BC, 8BC and 10BC treatments, respectively. Combining stable isotope technology, qPCR analysis and high-throughput sequencing, five microbial pathways of N2O production, including bacterial and archaea nitrification (BN and AN), denitrification performed by fungi, denitrifier bacteria and nitrifier bacteria (FD, DD and ND), were roughly distinguished. In addition, the extent of N2O reduction was obtained by δ18O vs.δ15NSP map. For all treatments, overall, the DD process (over 50%) was the main process of N2O production and reduction, while ND and AN processes were almost negligible (less 5%). In detail, the decrease of N2O emissions was caused by decreasing the contribution of FD in the 6BC, 8BC and 10BC treatments and reducing the contribution of BN in the 0BC and 2BC treatments. In addition, biochar addition increased the extent of N2O reduction to N2. In summary, the 2% biochar addition presented the greatest extent of N2O reduction to N2 (83%) and the lowest N2O emissions as well as the highest lettuce yields and nitrogen utilization efficiency. Therefore, 2% biochar is deemed the most optimal addition to the peat-based substrate.

Comparative biotic and abiotic effects on greenhouse gas emissions from agricultural ecosystems: application of straw or biochar?

Farmland has become a significant contributor to greenhouse gas (GHG) emissions, and research has shown that the addition of straw or biochar may be a viable method for mitigating these emissions. However, there is a lack of understanding regarding the comparative biotic and abiotic effects of straw and biochar amendments on GHG emissions. To address this knowledge gap, we conducted a meta-analysis of 100 published papers to quantify the impact of straw and biochar application on GHG emissions. Our findings indicate that straw application significantly increased CO2 and CH4 emissions from agricultural ecosystems by 46.2% and 113.5%, respectively, but did not have a significant effect on N2O emissions. Conversely, biochar amendment significantly reduced CO2, CH4, and N2O emissions by an average of 11.0%, 31.7%, and 22.8%, respectively. We also found that straw and biochar amendments increased soil pH, soil organic carbon (SOC), and C/N ratio, and there were significant differences between them. Moreover, straw application significantly increased the microbial biomass carbon (MBC) content and microbial quotient by 37.1% and 20.1%, respectively, while biochar application increased the MBC content by 25.0% without a significant effect on the microbial quotient. Furthermore, both straw and biochar applications promoted the nitrification process and increased the abundance of ammonia-oxidizing bacteria (AOB) by 50.7% with straw and by 57.5% and 75.1% with biochar for ammonia-oxidizing archaea (AOA) and AOB, respectively. The denitrification process was also stimulated by straw or biochar amendment, resulting in an increase in the abundance of nirK by 22.9% and 16.8%, respectively. Biochar amendment additionally increased the abundance of nosZ by 29.4%, indicating that the main reason for reducing N2O emissions through biochar application is the conversion of NO3--N to N2. Thus, compared to straw application, biochar application is a more effective method for reducing greenhouse gas emissions.

Reduction of sewage sludge and N2O emissions by an Oxic Settling Anaerobic (OSA) process: The case study of Corleone (Italy) wastewater treatment plant

Biosolid management is becoming one of the most crucial issues for wastewater treatment plant (WWTP) operators. The application of the Oxic Settling Anaerobic (OSA) process allows the minimisation of excess sludge production. This study compares conventional activated sludge (CAS) and OSA layouts in a full-scale WWTP (namely, Corleone - Italy). Extensive monitoring campaigns were conducted to assess treatment performances regarding carbon and nutrient removal, greenhouse gas (GHG) emissions, excess sludge production, and biomass activity (by means of respirometric analysis). Results showed that the effluent quality consistently met the Italian discharge limits. However, with the implementation of the OSA process, there was a decrease in ammonium removal efficiency, which could be attributed to reduced nitrifier activity related to reduced biomass production and extended anaerobic conditions affecting the nitrification process. On the other hand, the OSA configuration significantly increased phosphorus removal, indicating a high phosphorus content in the resulting waste sludge. A worsening of the sludge settling properties was observed with the OSA configuration likely due to decreased EPS concentrations. The sludge production in the OSA configuration decreased by 17.3 % compared to CAS. Nitrous-oxide measurements did not show a variation between CAS and OSA configurations, confirming that the OSA process can be a suitable solution for reducing WWTP's carbon footprint.

Cascade NH3 Oxidation and N2O Decomposition via Bifunctional Co and Cu Catalysts.

The selective catalytic oxidation of NH3 (NH3-SCO) to N2 is an important reaction for the treatment of diesel engine exhaust. Co3O4 has the highest activity among non-noble metals but suffers from N2O release. Such N2O emissions have recently been regulated due to having a 300× higher greenhouse gas effect than CO2. Here, we design CuO-supported Co3O4 as a cascade catalyst for the selective oxidation of NH3 to N2. The NH3-SCO reaction on CuO-Co3O4 follows a de-N2O pathway. Co3O4 activates gaseous oxygen to form N2O. The high redox property of the CuO-Co3O4 interface promotes the breaking of the N-O bond in N2O to form N2. The addition of CuO-Co3O4 to the Pt-Al2O3 catalyst reduces the full NH3 conversion temperature by 50 K and improves the N2 selectivity by 20%. These findings provide a promising strategy for reducing N2O emissions and will contribute to the rational design and development of non-noble metal catalysts.

Measurements and simulations on effects of elevated pressure and strain rate on NOx emissions in laminar premixed NH3/CH4/air and NH3/H2/air flames

Efforts to reduce NOx emissions by utilizing ammonia-fired combustors are made through various methods; this work investigates the possibility of combining the effects of pressure and stretch rate to reduce NOxemissions in laminar premixed flames. NOx emissions, including NO, NO2, N2O, NH3,and HCN, from NH3/CH4/air and NH3/H2/air unstrained and strained laminar premixed flames were separately measured and simulated with a high-pressure heat flux burner and CHEMKIN software. The key points are that increasing the pressure can decrease most NOx emissions, including NO, N2O, and HCN. However, it slightly increases the unburnt ammonia emissions and largely increases the NO2 formation. Medium XNH3 conditions are the most favorable for reducing NO emissions by increasing pressure. High strain rates can reduce NO emissions, but only in stoichiometric and rich conditions; they reversely increase NO emissions for lean equivalence ratios before the NO peak equivalence ratio (ϕ). The reason is explained by kinetic and reaction pathway analyses, which showed that in high-strain flames, more fuel-N species participated in the reaction to form N2 instead of NO for higher ϕ, while O and OH increased for lower ϕ, promotingNO formation. The combination of increasing pressure and strain rate can efficiently reduce NO emissions but ensure full ammonia oxidation for the medium ammonia content at ϕ = 0.9 ∼ 1.1. This sheds light on the utilization of highly strained ammonia-blend flames in more practical high-pressure burners to reduce NOx emissions.

Sustainability of hydrogen-enriched methane MILD combustion over a wide range of hydrogen-blending ratios in a strongly heat-extracted well-stirred reactor

Moderate or intense low-oxygen dilution (MILD) combustion of methane blended with hydrogen offers a potential solution for gas-fired industrial furnaces and even boilers to realize rapid decarbonization while maintaining ultra-low NO emissions. In this work, the combustion mode map for identifying MILD combustion when burning CH4/H2 mixtures from pure methane to pure hydrogen is proposed, and then the sustainability of hydrogen-enriched methane MILD combustion over a wide range of hydrogen-blending ratios from 0 to 100% under strongly heat-extracted conditions is revealed. Results show that, there exists a threshold oxygen mole fraction (XO2*) below which hydrogen-enriched methane MILD combustion can be achieved as long as the inlet temperature (Tin) is above the self-ignition temperature of reactants. As the hydrogen-blending ratio increases from 0 to 100%, XO2* is reduced from 9.3 to 6.5% and thus the unconditional MILD combustion region is concentrated, implying that hydrogen addition requires a larger oxygen dilution level to sustain MILD combustion. Interestingly, the sustainable operating range of oxygen concentration to sustain hydrogen-enriched methane MILD combustion can be extended by increasing the heat extraction ratio (HER) properly, e.g., XO2* up to 13.2% at HER = 30% for pure hydrogen fuel. However, methane MILD combustion would become unstable and even extinguished once HER exceeds about 49%, whereas MILD combustion of methane blended with a high percentage of hydrogen could be sustained even under highly heat-extracted conditions, e.g., a maximum allowable HER of about 91% for pure hydrogen fuel. In pure hydrogen MILD combustion, heat extraction can further reduce NO emissions, mainly due to less NO production via the N2O-intermediate, NNH, and thermal pathways, and NO reduction by H radicals tends to be weakened with heat extraction. In conclusion, high-percentage hydrogen co-firing makes it possible to achieve stable MILD operation with low NO emissions under strongly heat-extracted conditions encountered in boilers.

Effect of Streptomyces JD211 application on soil physicochemical properties and N2O emission characteristics of rice rhizosphere

Biocontrol agent, as a pollution-free and sustainable plant disease control method, can inhibit the spread of soil-borne diseases and promote the growth of crops. However, there are few studies on the effect of biocontrol agent on N2O emission in rice soil. In this study, after the application of the biocontrol agent Streptomyces JD211, N2O emission from rice soil were measured, and the relationship between the agent and soil N2O emissions were studied in soil chemistry and molecular biology. The results showed that the application of Streptomyces JD211 can significantly reduce the rate of N2O emission from rice soil. The NH4+-N and NO3−-N contents in rice soil decreased in a short period of time after the application of Streptomyces JD211, while the mineral N content in the soil remained stable with rice growth. 16S rRNA gene sequencing and metagenomic sequencing revealed Streptomyces JD211 application mainly increased the relative abundance of Burkholderia and Streptomyces in the soil microbial community, reduced the relative abundance of hao, norB, norC genes, and increased the relative abundance of nosZ and hcp genes. Streptomyces JD211 application promoted N2O transformation and weakened N2O production pathways, which ultimately reduced N2O emissions from rice soils. This study provided new insight of biocontrol agents to regulate soil N2O emissions, which is of great significance for the development and application of biocontrol bacteria and farmland environmental protection.

Nitrogen removal and nitrous oxide emission in the partial nitritation/anammox process at different reflux ratios

The partial nitritation/anammox (PN/A) process has been widely used in wastewater treatment owing to its notable advantages, including a low aeration rate and the non-requirement of an additional carbon source. In practical implementation, nitrite accumulation affects the nitrogen-removal efficiency and the amount of N2O released during the PN/A process. By implementing wastewater reflux, the nitrite concentration can be decreased, thereby achieving a balance between the nitrogen-removal efficiency and N2O release. This study conducted the CANON process with varying reflux ratios of 0 to 300 % and ~300 mg/L ammonium in the influent. The highest removal efficiency of ammonium and total nitrogen (98.2 ± 0.8 and 77.8 ± 2.3 %, respectively) could be achieved at a reflux ratio of 200 %. Further, a reflux ratio of 200 % led to the lowest N2O emission factor (2.21 %), with a 31.74 % reduction in N2O emission compared to the process without refluxing. Additionally, the reactor at a reflux ratio of 200 % presented the highest relative abundance of anaerobic ammonium-oxidizing bacteria (30.98 %) and the lowest proportion of ammonium-oxidizing bacteria (9.57 %). This study aimed to elucidate the impact of the reflux ratio on the nitrogen-removal efficiency of the CANON process and to theoretically explain the influence of different reflux ratios on N2O release. These findings provide a theoretical framework for enhancing the nitrogen-removal efficiency and mitigating carbon emissions in practical applications of the CANON process.

Study on the mechanism of nitrapyrin microcapsule suspension effectively improving nitrification inhibition rate in black soil

Nitrification inhibitors (NIs) have been widely applied to inhibit nitrification and reduce N2O emissions in agriculture. However, there are still some shortcomings, e.g. short effective periods, large applying amounts, low effectiveness, easy deactivation and different effect. Thus, a nitrapyrin microcapsule suspension (CPCS) was used as a new experimental material to elaborate its effects on nitrogen transformation and microbial response mechanisms in black soil by cultivation experiments with six treatments of no fertilization (CK), urea, urea+ 0.2 % CPES, urea+ 0.1 % CPCS, urea+ 0.2 % CPCS, and urea+ 0.3 % CPCS. The content of ammonium, nitrate nitrogen, functional microbial activity, degradation rate and adsorption characteristics of CPCS in the soil at different incubating times were determine. Compared with the nitrapyrin emulsifiable concentrate (CPEC) treatment, the degradation rate of CPCS decreased by 21.54 %, the half-life increased by 10.2 days, and the adsorption rate of nitrapyrin on black soil decreased more than 6-fold. CPCS effectively inhibited the transformation of ammonium nitrogen to nitrate nitrogen within more than 42 days. CPCS had a negative effect on amoA gene abundance and a positive effect on nrfA gene abundance. The research results provide a basic theoretical support for the application of CPCS on black soil.

Mitigation of N2O emission from granular organic fertilizer with alkali- and salt-resistant plant growth-promoting rhizobacteria.

Organic fertilizer application significantly stimulates nitrous oxide (N2O) emissions from agricultural soils. Plant growth-promoting rhizobacteria (PGPR) strains are the core of bio-fertilizer or bio-organic fertilizer, while their beneficial effects are inhibited by environmental conditions, such as alkali and salt stress observed in organic manure or soil. This study aims to screen alkali- and salt-resistant PGPR that could mitigate N2O emission after applying strain-inoculated organic fertilizer. Among the 29 candidate strains, 11 (7 Bacillus spp., 2 Achromobacter spp., 1 Paenibacillus sp., and 1 Pseudomonas sp.) significantly mitigated N2O emissions from the organic fertilizer after inoculation. Seven strains were alkali tolerant (pH 10) and five were salt tolerant (4% salinity) in pure culture. Seven strains were selected for further evaluation in two agricultural soils. Five of these seven strains could significantly decrease the cumulative N2O emissions from Anthrosol, while six could significantly decrease the cumulative N2O emissions from Cambisol after the inoculation into the granular organic fertilizer compared with the non-inoculated control. Inoculating alkali- and salt-resistant PGPR into organic fertilizer can reduce N2O emissions from soils under microcosm conditions. Further studies are needed to investigate whether these strains will work under field conditions, under higher salinity, or at different soil pH.