The effectiveness of treatments of winter wheat crops with liquid humic preparations “EKOSS” and “BIO-Don” has been studied. Humic preparations were used as a working solution with a concentration of 0.002% (by carbon). The leaf apparatus of winter wheat was processed once (during the tillering phase) and twice (during the tillering and heading phases). The control was the variant without the introduction of humic preparations against the background of an average level of mineral nutrition N40P40K40 with the use of soil treatment technologies and cultivation methods adopted for this crop in the region. Soil sampling was carried out 4 times during the tillering (twice), heading phases and after harvest during 2021-2022. The use of humic preparations was accompanied by an increase in the activity of urease. In different weather conditions of the year, urease activity differs, but always increases from the beginning of the spring vegetation until the moment of harvesting. In favorable weather conditions in 2021, urease activity is weaker than in dry 2022, which indicates a positive effect of humic preparations under stressful conditions, where they show their adaptogenic properties. Double treatment had a better effect on the nitrogen nutrition of plants. At the same time, the humic preparation “EKOSS” had a greater effect on the nitrogen regime of plants than “BIO-Don”.