Protocol for the fixation of nitrogen from air using focused ultrasound.

Nitrogen fixation in plasma integrated mixed ionic electronic conducting membrane reactors

Perfluorooctanoic acid at environmentally relevant concentrations interfere with nitrogen fixation and amino acid synthesis in soil-soybean system.

Differential effects of sulfide-induced transformation of biodegradable and conventional microplastics on sedimentary CO2 and CH4 emissions: Underlying microbiome-mediated mechanisms.

Divergent edaphic filters drive ecosystem-specific microbiome assembly and metabolic strategies in Northern China's transitional zone.

One-pot in situ synthesis of oxygen vacancy-rich Bi3O4Br/Bi2O2CO3 S-scheme heterojunction for highly efficient photocatalytic nitrogen fixation.

This article presents the draft genome sequence of Methanosarcina mazei strain OFF1024, isolated from paddy field sediments of Pondicherry, India. The genome was sequenced using the Illumina HiSeq PE150 platform and assembled de novo with MEGAHIT, resulting in a 4.07 Mb genome comprising 260 contigs with a GC content of 41.56%. Genome annotation identified 3459 coding sequences, 44 tRNA genes, and 3 rRNA genes. Comparative genomic analysis using TYGS confirmed the strain's placement within the M. mazei species cluster, supported by high dDDH similarity to M. mazei C16 and M. mazei S-6. Functional gene mining revealed the nitrogen fixing gene set, including nitrogenase structural subunits, regulatory components, hydrogenases, and Fe-Mo cofactor biosynthesis genes, indicating the diazotrophic potential of this methanogen. The genome additionally encodes diverse methanogenesis pathways and stress-response mechanisms relevant to flooded agroecosystems. This dataset provides a valuable genomic resource for studying methanogenic archaea in paddy soils, supporting future research on methane cycling, archaeal ecology, nitrogen fixation, and bioenergy applications.

Cyanolichen crusts are a key predictor of nitrogen cycling and fixation potential in soils from semiarid Mediterranean ecosystems

Synergistic catalysis of hybrid discharges for sustainable nitrogen fixation via nanosecond pulse-driven underwater bubble systems

Transition metal doping-induced defect engineering in Bi4O5Br2 for enhanced photocatalytic nitrogen fixation

Coulomb repulsion induced symmetry-breaking in BaZrO3 perovskites for triggering piezo-photocatalytic nitrogen fixation reaction

Summary statement Research reveals that DCPTA treatment boosts soybean nodulation and pinpoints the nodule‐specific gene GmATL31 as essential for this process, where it functions by enhancing symbiotic signaling and nutrient metabolism. These findings offer a genetic strategy and theoretical foundation for using DCPTA to improve biological nitrogen fixation in legumes, potentially reducing reliance on chemical fertilizers.

Soybean ( Glycine max ) root nodules, formed through symbiosis with nitrogen-fixing rhizobia, are essential for biological nitrogen fixation. While quantifying key nodulation traits, nodule number and weight, is critical for assessing symbiotic efficiency and yield potential, current methods are destructive and labor-intensive, unsuitable for longitudinal monitoring and high-throughput phenotyping. Here, we established hyperspectral leaf reflectance as a non-destructive, high-resolution tool capable of monitoring root nodule development. Using Partial Least Squares Regression models, we connected spectral data with nodule metrics from 528 unique soybean plants across 18 genotypes, inoculated with different rhizobium strains, and under different abiotic stresses. These models achieved high accuracy for predicting nodule number (R 2 = 0.75, nRMSE = 6.02%) and moderate accuracy for nodule weight (R 2 = 0.53, nRMSE = 12.38%). Crucially, spectral analyses revealed distinct hyperspectral signatures sensitive to nodule traits. While different rhizobium strains induced comparable changes in both nodule traits, and therefore produced highly overlapped spectral domains, diagnostically distinct spectral patterns were generated under drought versus salt stress, with the former suppressing nodulation more significantly than the latter. Furthermore, we demonstrated the effectiveness of our models for real-time in-situ monitoring of nodule development for individual plants. Spectral-nodule trait covariation analyses further revealed leaf signatures correlated with nodule traits primarily through systemic physiological coupling governed by carbon-nitrogen exchange dynamics and plant water status. This study showcased hyperspectral sensing as a transformative methodology, enabling the unprecedented non-destructive quantification of nodulation dynamics, revealing novel physiological insights into plant-microbe-environment interactions, facilitating breeding and management strategies for sustainable soybean production.

Co-inoculation of arbuscular mycorrhizal fungi and rhizobia reshapes microbial ecology and nutrient metabolism to rehabilitate iron ore tailings.

Synergistic engineering of oxygen vacancies and Schottky junctions for enhanced solar-driven nitrogen fixation on hierarchical hollow Bi4Ti3O12

Single-atom Ti doping on boron nitride monolayers with different defects as an efficient catalyst for nitrogen fixation

Zn-induced energy-level and polarization coupling enables highly efficient photo-piezocatalytic nitrogen fixation over layered PbBiO2Cl

Sweet potatoes were cultivated in hydroponics with low nitrogen fertilization. Through 16S rRNA gene amplicon sequencing, the most abundant genera in their storage roots were identified as Azospirillum, Sphingomonas, Burkholderia, Bradyrhizobium, and Arthrobacter, all of which have been reported as nitrogen-fixing bacteria. These results suggest that nitrogen-fixing bacteria may selectively increase to compensate for insufficient nitrogen fertilization.

Over the past decades, the intensive use of chemical fertilizers in agriculture has shown low efficiency while causing serious environmental issues and leading to soil nutrient imbalances. These challenges are compounded by climate change, increasing incidence of diseases and pests, and soil acidification, factors that jeopardize agricultural productivity and, consequently, threaten global food security. Soybean (Glycine max L.) is one of the world's most important crops, serving as a key source of protein and oil for both human consumption and animal feed. Its global relevance continues to grow with rising demand for food, biofuels, and industrial applications, with Brazil, the United States, and Argentina leading production. Beyond its economic value, soybean contributes to agricultural sustainability through symbiotic nitrogen fixation, reducing the need for synthetic fertilizers. However, maintaining high yields under changing environmental conditions requires innovative management strategies. In this context, one promising strategy to mitigate these problems is the use of plant growth-promoting bacteria (PGPB), which contribute to more sustainable crop yield. Although numerous studies are underway regarding the potential of PGPB, further research is still necessary due to the limited understanding of their mechanisms of action and the vast range of benefits they may offer. Currently, there is a wide variety of inoculants based on different bacterial species, which play a key role in stimulating plant growth and reducing reliance on agrochemicals. Among emerging technologies, noteworthy examples include molecular inoculants (still not widely adopted commercially), bacterial and fungal consortia formulated into a single product, and inoculants containing genetically edited microorganisms-all of which have shown great promise in enhancing the performance of beneficial microbial species. The selection and genetic editing of rhizosphere-associated PGPB-an essential component of the plant microbiome-are viable alternatives for promoting more sustainable agriculture. Thus, this review examines the main inoculant technologies aimed at obtaining efficient microorganisms capable of improving rhizosphere conditions and microbial community dynamics, representing a strategic opportunity for developing solutions that enhance soybean sustainability.

This study evaluated methanogenic effluent combined with nitrogen-fixing bacteria (NFB) strain BSN6 as a sustainable nutrient solution for hydroponic green oak lettuce (Lactuca sativa var. crispa L.) cultivation. A preliminary experiment assessed the NFB effects on germination, while the main experiment tested six effluent concentrations (0, 2.5, 5, 10, 15, and20%) with and without the NFB using a completely randomized design. The 2.5% effluent combined with the NFB produced optimal growth, achieving 16.66 ± 0.95cm plant height, 25.60 ± 0.60 leaves, and 39.74 ± 3.21g fresh weight, which was comparable to commercial AB Mix fertilizer. Higher concentrations (15-20%) caused plant toxicity, with 20% + the NFB resulting in complete mortality. The NFB enhanced nitrogen availability at low concentrations but proved ineffective at higher levels. A preliminary safety assessment showed no detectable copper or chromium in lettuce tissue. This approach offers environmentally sustainable effluent management while reducing synthetic fertilizer dependence, demonstrating significant potential for integrated biogas-agriculture systems.