Neutral Properties Research Articles

Soluplus® nanomicelles enhance IgG neutralizing properties against Shiga toxin type 2

Shiga toxin (Stx) is the main virulence factor of Stx-producing Escherichia coli, a pathogen responsible for developing Hemolytic Uremic Syndrome. Nowadays, no specific treatment exists for this disease, and nanomicelles may be an excellent platform for the delivery of specific compounds, including immunoglobulins (IgG), for therapeutic or diagnostic strategies. In this work, Soluplus® nanomicelles (NM) were associated with IgG against Stx type 2 (NM-IgG-Stx2). Physicochemical evaluation of NM-IgG-Stx2 was performed by Dynamic Light Scattering, Uv–Vis, Fluorescence, and Circular Dichroism spectroscopy. Morphology was analyzed by transmission electron microscopy. An in silico analysis of NM-IgG-Stx2 association was performed, and neutralization properties against purified Stx2 and Stx-derived supernatants were analyzed on Vero and HGEC cells. Our results suggest that IgG-Stx2 assemble with NM, and the interaction improves the Stx2 neutralization capacity of the antibody. These results suggest that NM may enhance IgG-Stx2 paratope availability and may be an excellent platform for the recognition and neutralization of Stx2.

Comparison of acidity and chemical composition of summertime cloud water and aerosol at an alpine site in Northwest China: Implications for the neutral property of clouds in the free troposphere

Aerosol and cloud acidity are essential to human health, ecosystem health and productivity, as well as climate effects. The main chemical composition of cloud water greatly varies in different regions, resulting in substantial differences in the pH of cloud water. However, the influences of the anthropogenic emissions of acidic gases and substances, alkaline dust components, and dicarboxylic acids (diacids) on the ground concerning the acidity of cloud water in the free troposphere of the Guanzhong Plain, China, remain clear. In this study, cloud water and PM2.5 samples were simultaneously collected in the troposphere (Mt. Hua, 2060 m a.s.l). The results indicated that the cloud water was alkaline (pH = 7.6) and PM2.5 was acidic (pH = 3.2). These results showed the neutral property of clouds collected in the heavily polluted Guanzhong Plain, although most previous studies always considered acidity as a marker of pollution. The sulfate (SO42−), nitrate (NO3−), and ammonium (NH4+) (SNA) of particulate matter and cloud water in the same period were compared. SO42− was dominant in particulate matters (accounting for 63.4 % of the total SNA) but substantially decreased in cloud water (only 30.1 % of the total SNA), whereas NO3− and NH4+ increased from 28.5 % and 8.2 % to 39.8 % and 30.2 %, respectively. This could be attributed to the complex formation mechanism and sources of SO42− and NO3− in the cloud. The results of ion balance indicated that a significant deficit of inorganic anion equivalents was observed in the cloud water samples. The high concentration of diacids in the cloud phase (1237.4 μg L−1) may facilitate the formation of salt complexes with NH4+, thus influencing the acidity of the cloud water. The pH of cloud water has increased in recent decades due to the sustained reduction of sulfur dioxide, which may also affect the acidity of future precipitation.

In-silico screening of bioactive compounds from nut grass (Cyperus rotundus) to inhibit the α-bungarotoxin from Kraits (Bungarus spp.) venom

ABSTRACT Kraits (Bungarus spp.) are the snakes that commonly cause fatal bites in Tropical Asia due to their neurotoxic venom. The complex process of antivenom production contributes to the lack of available antivenom and amplifies the burden of snakebite in that region. Thus, the victims seek traditional medicine such as root extract from nut grass (Cyperus rotundus). In the present study, we utilized bioinformatics methods to analyze the potential inhibitory activity of bioactive compounds from nut grass (Cyperus rotundus) against kraits’ α-bungarotoxin. Twenty-one bioactive compounds were screened using ADMET, membrane permeability prediction, molecular docking and molecular dynamics simulations. Among these bioactive compounds, we found that alpha-cyperone, cyperol, cyperusol, rotundine A, rotundine B and 4,6,3’,4’-Tetramethoxyaurone had inhibitory activity by presenting a good ability to pass phospholipid bilayer and could bind stably into active sites of α-bungarotoxin with minimum energy required. Importantly, the neutralization property of these bioactive compounds resides in their combinational function, underscoring the crucial significance of their combined action rather than individual contributions. In conclusion, this study identifies a combination of six bioactive compounds from C. rotundus with a potential as an alternative antivenom based on in-silico screening and provides essential data for the development of a plant-based antivenom.

Autologous cryo-shocked neutrophils enable targeted therapy of sepsis via broad-spectrum neutralization of pro-inflammatory cytokines and endotoxins.

Background: Sepsis is a life-threatening disease characterized by multiple organ failure due to excessive activation of the inflammatory response and cytokine storm. Despite recent advances in the clinical use of anti-cytokine biologics, sepsis treatment efficacy and improvements in mortality remain unsatisfactory, largely due to the mechanistic complexity of immune regulation and cytokine interactions. Methods: In this study, a broad-spectrum anti-inflammatory and endotoxin neutralization strategy was developed based on autologous "cryo-shocked" neutrophils (CS-Neus) for the management of sepsis. Neutrophils were frozen to death using a novel liquid nitrogen "cryo-shock" strategy. The CS-Neus retained the source cell membrane structure and functions related to inflammatory site targeting, broad-spectrum inflammatory cytokines, and endotoxin (LPS) neutralizing properties. This strategy aimed to disable harmful pro-inflammatory functions of neutrophils, such as cytokine secretion. Autologous cell-based therapy strategies were employed to avoid immune rejection and enhance treatment safety. Results: In both LPS-induced sepsis mouse models and clinical patient-derived blood samples, CS-Neus treatment significantly ameliorated cytokine storms by removing inflammatory cytokines and endotoxin. The therapy showed notable anti-inflammatory therapeutic effects and improved the survival rate of mice. Discussion: The results of this study demonstrate the potential of autologous "cryo-shocked" neutrophils as a promising therapeutic approach for managing sepsis. By targeting inflammatory organs and exhibiting anti-inflammatory activity, CS-Neus offer a novel strategy to combat the complexities of sepsis treatment. Further research and clinical trials are needed to validate the efficacy and safety of this approach in broader populations and settings.

CeO2-based peelable gel for neutralization and skin decontamination toward chemical warfare agents

Accidents with toxic industrial chemicals and threats posed by chemical warfare agents, as organophosphorus nerve agents and blistering agents, has become an increasing concern over the last decades. Intoxication is not only due to inhalation but dermal route can also be important and can quickly lead to death. It is therefore crucial to have suitable means of personal protection and decontamination. Some are already commercially available but often present drawbacks. Efforts have been made to develop new systems and several studies have shown the interest of metallic nanoparticles. The challenge remains to entrap the particles in an adapted matrix to limit their diffusion in the immediate environment, while preserving the bare particles decontamination efficacy. In this study, an easy to handle peelable gel, incorporating CeO2 nanoparticles has been developed for efficient skin decontamination against toxic chemicals. The main objective has been to make it suitable in any context of application (battlefield or civil exposure). Nano-octahedra (NO) CeO2 particles synthetized in this study have been able to increase the degradation of paraoxon, a simulant warfare agent in liquid media. NO have then been incorporated in a peelable gel, the formulation has been optimized through design of experiments and its physico-chemical properties characterized. Even after incorporation to the gel, NO retained their neutralization properties. The formulation efficiency towards neutralization and skin decontamination have been evaluated in vitro on Franz diffusion cells. The interest of the peelable gel have been clearly demonstrated with a decrease by about 5-fold of the quantity of POX absorbed into the skin and a decrease of 3–4-fold in the viable skin, which was much higher than the use of cotton or nanoparticles as powder.

SARS-CoV-2 Neutralization by Cell Membrane-Coated Antifouling Nanoparticles.

The global outbreak of the COVID-19 pandemic has indisputably wreaked havoc on societies worldwide, compelling the scientific community to seek urgently needed therapeutic agents with low-cost and low-side effect profiles. Numerous approaches have been investigated in the quest to prevent or treat COVID-19, but many of them exhibit unwelcome side effects, such as dysfunctional viral immune responses and inflammation. Herein, we present the preparation of solid natural human pulmonary alveolar epithelial cell (ATII) membrane-coated PLGA NPs (PLGA NPs@ATII-M), which demonstrate remarkable affinity and competitiveness to neutralize the SARS-CoV-2 S1 protein-coated NPs (SCMMA NPs-S1), which are employed as a surrogate for coronavirus particles. In addition, we first considered the antifouling properties of these types of NPs, and we found that this membrane-coated NP formulation boasts excellent antifouling capabilities, which serve to protect their neutralization properties out of shielding by protein coronas in blood circulation. Moreover, this formulation is easily prepared and stored with a low-cost profile and exhibits good specificity, high targeting efficiency, and potentially side effect avoiding, thus making it a highly promising candidate for COVID-19 treatment.

SARS-CoV-2 Specific Nanobodies Neutralize Different Variants of Concern and Reduce Virus Load in the Brain of h-ACE2 Transgenic Mice.

Since the beginning of the COVID-19 pandemic, there has been a significant need to develop antivirals and vaccines to combat the disease. In this work, we developed llama-derived nanobodies (Nbs) directed against the receptor binding domain (RBD) and other domains of the Spike (S) protein of SARS-CoV-2. Most of the Nbs with neutralizing properties were directed to RBD and were able to block S-2P/ACE2 interaction. Three neutralizing Nbs recognized the N-terminal domain (NTD) of the S-2P protein. Intranasal administration of Nbs induced protection ranging from 40% to 80% after challenge with the WA1/2020 strain in k18-hACE2 transgenic mice. Interestingly, protection was associated with a significant reduction in virus replication in nasal turbinates and a reduction in virus load in the brain. Employing pseudovirus neutralization assays, we identified Nbs with neutralizing capacity against the Alpha, Beta, Delta, and Omicron variants, including a Nb capable of neutralizing all variants tested. Furthermore, cocktails of different Nbs performed better than individual Nbs at neutralizing two Omicron variants (B.1.529 and BA.2). Altogether, the data suggest the potential of SARS-CoV-2 specific Nbs for intranasal treatment of COVID-19 encephalitis.

Ionic liquid dopant induced 3D hierarchical CuO nanostructures with doped heteroatoms and highly dispersed Ag for electrochemical upgrading of 5-hydroxymethylfurfural

The electrocatalytic 5-hydroxymethylfurfural oxidation reaction (HMFOR) has received increasing attention due to its carbon–neutral and value-added chemical properties, and the development of electrocatalyst with highly active and selective is crucial. Highly dispersing metal atoms throughout the catalyst can maximize the catalytic efficiency. Here, we synthesized a 3D hierarchical CuO nanostructure induced by ionic liquid with heteroatoms doping and Ag dispersing (Ag-CuO@IL), and this self-supported catalyst can reach 10 mA cm−2 at only 1.33 V vs RHE and achieved HMF conversion of 98.5 %, FDCA yield of 97.1 % and Faraday efficiency of 92.2 %. The excellent catalytic performance of Ag-CuO@IL for HMFOR is attributed to the doped heteroatoms derived from ILs to promote electron redistribution and the generated oxygen vacancies induced by IL anions to highly disperse Ag. Also, the catalyst was the nanosheet-assembled spherical clusters with 3D nanostructure, which exposed a large number of active sites. Density functional theory calculations showed that Ag-CuO@IL possessed moderate adsorption strengths of HMF and OH–, thus facilitating the desorption of the products in the reaction process. The design that induced by ionic liquid dopant not only provides an effective and green mean for HMFOR, but also has the large potential to guide the synthesis of other catalysts with improved performances in various applications.

Increased breadth and neutralization of antibodies against SARS-CoV-2 variants after infection and vaccination: A serosurveillance study in pediatric patients of Southern Switzerland.

Little information is available about the nature of the immune response in children after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or vaccination. The aim of this study is to define the seroprevalence and the features of the antibody response in children of Southern Switzerland during the different waves of Coronavirus Disease 2019 (COVID-19) pandemic.By analyzing 756 sera collected from children aged 0 to 16years admitted to the Institute of Pediatrics of Southern Switzerland during the prepandemic period (before March 2020) and the first four pandemic waves (between March 2020 and June 2022), we investigated binding titers, cross-reactivity, and neutralizing properties of the serum antibodies against SARS-CoV-2 variants.Seroprevalence varied from 6% during the first wave to 14% and 17% during the second and third waves, respectively, peaking at 39% during the fourth wave. The 96 seropositive cases were mostly asymptomatic (42.7%) or showed mild (20.8%) to moderate (32.3%) symptoms. Moderate symptoms and close contact with COVID-19-positive individuals were associated with a higher infection risk (P < 0.001). The antibody response was mainly driven by IgG directed to the receptor-binding domain (RBD) of Wuhan-1 SARS-CoV-2 Spike (S). Children infected in the first three waves produced antibodies with up to 11-fold and 5.5-fold reduction in binding and neutralizing titers, respectively, against different SARS-CoV-2 variants, including Beta, Delta, and Omicron BA.1, BA.2, and BA.5. Such reductions were less pronounced in children infected during the fourth wave, who showed the highest frequency and titers of neutralizing antibodies against the same variants. Compared to infection, vaccination with a Wuhan-1-based messenger RNA (mRNA) vaccine induced higher and heterogenous levels of antibodies cross-reacting to the different SARS-CoV-2 variants analyzed. Conclusions: Despite the high burden of COVID-19 in Southern Switzerland, we observed an initial low seroprevalence of SARS-CoV-2 in children, which increased in the later waves. The antibody response was poor in the first three waves and improved in the fourth wave, when children produced higher levels of neutralizing antibodies after vaccination or infection with Delta and/or Omicron variants. What is Known: • Children were marginally affected by the initial SARS-CoV-2 variants. • The number of infected and hospitalized children increased after the appearance of the Omicron variants. What is New: • Seroprevalence of SARS-CoV-2 in children of Southern Switzerland increased overtime. • Children produced higher levels of neutralizing antibodies after vaccination or infection with Delta and/or Omicron variants in the fourth wave compared to children infected in the first three waves.

Evaluation of In Vitro Acid Neutralizing Capacity of Bilwadi Vati

Herbs have been used for thousands of years in traditional medicine systems, such as Ayurveda, to treat various ailments, including hyperacidity. The aim of this study was to investigate the acid neutralizing properties of Bilwadi Vati, a polyherbal formulation used to treat the intestinal disorders. A total of 14 herbal ingredients of Bilwadi Vati were tested using an in vitro assay to determine their Preliminary antacid test andacid neutralizing capacity. The results showed that Bilwadi Vati as a whole and all the herbal ingredients tested exhibitacid neutralizing properties to varying degrees. Among the tested herbal ingredients Sunthi (Zingiber officinale), Maricha (Piper nigrum), Nata (Valerianawalichi), Haridra (Curcuma longum) and Daruharidra (Berberis aristata) five ingredients demonstrated the significantly higher acid neutralizing capacity. This study supports and sheds light on the purported digestive and antiacidic properties of Bilwadi Vati and its individual ingredients. Further study to determine the in vitro neutralizing and buffering capacity of ingredients is in process.

Plasma-thermal remelting of ash and slag waste from waste incineration plants

This work is devoted to the study of the process of processing ash residues from a waste incineration plant (WIP) using a computational and experimental approach. The purpose of this work is to prevent environmental pollution by melting WIP ash and slag and obtaining a useful product with neutral properties. To achieve this goal, a combined method of heating ash and slag was proposed.

Preventive geochemical barriers during transport construction

The article discusses the possibility of using chlorite crushed stone as part of geochemical barriers in transport construction. It has been shown that during the construction of railways and during their operation, the main pollutants are petroleum products and heavy metal ions. To substantiate and study the neutralizing properties of chlorite crushed stone, infrared spectroscopy, ionomer, atomic absorption spectrometry and the gravimetric method for determining petroleum products were used. Research has shown that chlorite crushed stone is a quartz-albite-chlorite shale with a small inclusion of carbon; its physical and mechanical characteristics are not inferior to granite crushed stone. The oil capacity of chlorite crushed stone depends on the concentration of petroleum products in the model solution and ranges from 0.03 to 0.5 g/g. Chlorite crushed stone has a capacity towards nickel, iron and cadmium ions, while their simultaneous presence in the model solution does not have selectivity towards these ions. It has been proven that when the capacity of chlorite crushed stone is fully realized in relation to one of the heavy metal ions, it is not washed out and replaced by another metal ion (for example, nickel and iron ions). It has been proven that the presence of organic contaminants in the model solution does not significantly affect the capacity of chlorite crushed stone for heavy metal ions. It has been shown that chlorite crushed stone, due to its physical, physical-mechanical and chemical properties, can be used in geochemical barriers to protect the environment from pollution.

Hydrochemical characterization of groundwater for consumption and agriculture: A case study from Phulpur Upazila, Bangladesh

The aim of this study was to assess the suitability of groundwater for drinking and irrigation in Phulpur Upazila, Bangladesh, based on its hydrochemical attributes. In our research, we examined twenty groundwater specimens from shallow and hand tube wells, adhering to international and national guidelines. The data showed groundwater pH levels from 5.67 to 6.84, signifying slightly acidic to neutral properties. Total dissolved solids ranged from 79 to 298 mg/l, and conductivity varied between 120 and 832 μS/cm. Notably, the primary cations and anions were sequenced as Ca &gt; Mg &gt; Na &gt; K and HCO3 &gt; Cl &gt; SO4 &gt; PO4, respectively. A marked correlation was discerned among the physicochemical parameters, and the predominant groundwater type was magnesium-bicarbonate (Mg-HCO3). The main geochemical activity was identified as silicate weathering, pointing to significant rock-water interactions in the region. The groundwater displayed moderate salinity and low alkalinity, qualifying them as 'excellent to good' and 'normal' in quality metrics, respectively. The soluble sodium percentage was rated as 'excellent'. On multiple assessment scales, the groundwater quality was deemed 'good' for irrigation. In line with established classification systems, most samples were suitable for drinking and irrigation. Hence, the area's groundwater is viable for consumption and agricultural utilization. Cumulatively, our research offers critical insights for sustainable groundwater stewardship in the region, providing a foundation for policymakers to guarantee the sustained provision of high-quality groundwater for diverse applications.

Effects of dimethyl ether blending on H2-fueled combustion characteristics and thermal improvement in fuel-lean condition

Micro-combustors play a pivotal role in micro powering system due to the potential for high energy density. However, addressing challenges such as incomplete combustion and high heat dissipation losses are essential for optimizing the working performance. So, the blended DME is proposed to micro-combustion, which acts as a promising oxygenated fuel with significant attention due to its higher reactivity, lower pollutant emissions, and carbon–neutral properties. However, the lack of dynamic models applicable to H2/DME fueled combustion in micro condition, so an optimized mechanism for blended H2/DME combustion is developed and validated. The results demonstrate that a reduced mechanism comprising 29 species and 106 reactions (M29-106*) can be commendably employed in micro H2/DME/air combustion simulation, according to the sensitivity analysis and reaction modification. Besides, investigation is conducted to explore micro-combustion characteristics of H2/DME/Air. The result shows that burning process and heat transfer are notably influenced by DME addition, leading to shifts in flame positions and adjustments in combustion reaction rates. So, DME addition enhances energy efficiency and modifies flame location through fluid field optimization and reduced heat loss. The thermal performance of the combustor is significantly improved with DME blending, resulting in higher radiative power and radiation efficiency. However, the choice of DME blending ratio must be carefully considered, as high blending ratios may compromise flame stability and lead to increased exhaust heat losses under fuel-lean conditions. Specifically, burning with 30 % DME addition achieves a radiative power of 20.24 W and a radiative efficiency of 40.57 % at the same chemical energy Ec = 49.9 W, which is 5.57 W and 11.17 % higher than that of pure H2-fueled combustion, respectively. These findings offer valuable insights for the design and optimization of micro-combustors, where compact and efficient power sources are required.

A cleavable chimeric peptide with targeting and killing domains enhances LPS neutralization and antibacterial properties against multi-drug resistant E. coli

Pathogenic Escherichia coli is one of the most common causes of diarrhea diseases and its characteristic component of the outer membrane-lipopolysaccharide (LPS) is a major inducer of sepsis. Few drugs have been proven to kill bacteria and simultaneously neutralize LPS toxicity. Here, the chimeric peptides-R7, A7 and G7 were generated by connecting LBP14 (LPS-targeting domain) with L7 (killing domain) via different linkers to improve antibacterial and anti-inflammatory activities. Compared to parent LBP14-RKRR and L7, the antibacterial activity of R7 with a cleavable “RKRR” linker and the “LBP14-RKRR + L7” cocktail against Escherichia coli, Salmonella typhimurium and Staphylococcus aureus was increased by 2 ~ 4-fold. Both A7 and G7 with non-cleavable linkers almost lost antibacterial activity. The ability of R7 to neutralize LPS was markedly higher than that of LBP14-RKRR and L7. In vivo, R7 could be cleaved by furin in a time-dependent manner, and release L7 and LBP14-RKRR in serum. In vivo, R7 can enhance mouse survival more effectively than L7 and alleviate lung injuries by selective inhibition of the NF-κB signaling pathways and promoting higher IAP activity. It suggests that R7 may be promising dual-function candidates as antibacterial and anti-endotoxin agents.

Tyrosinase inhibitory peptides: Structure-activity relationship study on peptide chemical properties, terminal preferences and intracellular regulation of melanogenesis signaling pathways

BackgroundBioactive peptides have gained attention as potential alternatives to chemical-based skin lightening agents. Based on literature search, the reported articles focused mainly on the sources and preparation methods of tyrosinase inhibitory peptides and there is lacking information regarding the structure-activity relationship (SAR) between peptide property and tyrosinase inhibition. It was hypothesized that peptide properties such as hydrophobicity/hydrophilicity and the amino acid type and position/arrangement at the terminal positions could affect peptide mode of binding hence result in various degrees of tyrosinase inhibition. MethodsIn this study, the sequences of 128 tyrosinase inhibitory peptides were collected from peer-reviewed articles. The hydrophobicity/hydrophilicity property and the amino acid profile of peptides at the N- and C-terminals were analyzed using bioinformatics tools. Molecular docking analysis was employed to further elucidate the roles of reactive amino acids in tyrosinase-peptide binding interactions. The peptide-regulated intracellular melanogenesis pathways were also compiled and discussed. ResultsIt was found that hydrophobic and/or polar neutral properties may facilitate or stabilize peptide binding with tyrosinase. Moreover, short peptides featuring a cysteine and tyrosine at the N- and C- terminal ultimate positions tend to bind to the active site of tyrosinase whereas positively charged amino acid such as arginine at the N-terminal does not favor peptide binding to tyrosinase. ConclusionsThese findings provide detailed explanation on how peptide/amino acid structures are related and what function they play in tyrosinase inhibition. It could also inspire researchers to account for tyrosinase-peptide SAR and the underlying anti-melanogenesis mechanisms in formulating peptide-based treatments or strategies against skin hyperpigmentation.

Способы образования парных терминов в кумыкском языке

The aim of the study is to reveal the peculiarities of the formation of paired terms in the Kumyk language. The author grouped paired terms according to the method of formation of the analysed terms. The paper demonstrates the attribution of the terms under consideration to a specific thematic group. The scientific novelty of the study lies in identifying the characteristics of paired terms in the Kumyk language. In the Kumyk language, a paired term cannot acquire expressive or stylistic connotations like commonly used paired words, due to its properties of exactness and neutrality. As a result, six types of paired terms were identified based on their formation method; the ways of translating the components of borrowed and calqued relative adjectives were described. In addition, the changes in terminological units borrowed from Russian were shown. In the Kumyk language, calquing and borrowing from the Russian language are most prevalent in the socio-political sphere. Paired terms in the Kumyk language are direct translations of Russian terminological concepts.

Mechanisms of Drug Solubility Enhancement Induced by β-Lactoglobulin-Based Amorphous Solid Dispersions.

Protein-based amorphous solid dispersions (ASDs) have emerged as a promising approach for enhancing solubility in comparison to crystalline drugs. The dissolution behavior of protein-based amorphous solid dispersions (ASDs) was investigated in various pH media. ASDs of four poorly soluble model drugs with acidic (furosemide and indomethacin), basic (carvedilol), and neutral (celecoxib) properties were prepared by spray drying at 30 wt % drug loading with the protein β-lactoglobulin (BLG). The effect of spray-dried BLG (SD-BLG) solubility and protein binding ability with dissolved drugs in solution were investigated to retrieve the mechanisms governing the improvement of drug solubility from the BLG-based ASDs. Powder dissolution results showed that all ASDs obtained a higher maximum concentration (Cmax) compared to the respective pure crystalline drugs. It was found that the solubility increase of the drugs from the ASDs was to a large extent dependent on the solubility of the pure SD-BLG at the investigated pH values (low solubility at pH near the isoelectric point (pI) of BLG). Furthermore, drug-protein interactions in a solution were observed, in particular at pH values where the drugs were neutral. These drug-protein interactions also resulted, to some extent, in the stabilization of the drug in supersaturation.

Classification of genotypes based on the VP1 gene of feline calicivirus and study of cross-protection between different genotypes.

Feline calicivirus (FCV) causes upper respiratory tract diseases and even death in cats, thereby acting as a great threat to feline animals. Currently, FCV prevention is mainly achieved through vaccination, but the effectiveness of vaccination is limited. In this study, 105 FCV strain VP1 sequences with clear backgrounds were downloaded from the NCBI and subjected to a maximum likelihood method for systematic evolutionary analysis. Based on the genetic analysis results, FCV-positive sera were prepared using SPF mice and Chinese field cats as target animals, followed by a cross-neutralization assay conducted on the different genotype strains and in vivo challenge tests were carried out to further verify with the strain with best cross-protection effect. The results revealed that FCV was mainly divided into two genotypes: GI and GII. The GI genotype strains are prevalent worldwide, but all GII genotype strains were isolated from Asia, indicating a clear geographical feature. This may form resistance to FCV prevention in Asia. The in vitro neutralization assay conducted using murine serum demonstrated that the cross-protection effect varied among strains. A strain with broad-spectrum neutralization properties, DL39, was screened. This strain could produce neutralizing titers (10 × 23.08-10 × 20.25) against all strains used in this study. The antibody titers against the GI strains were 10 × 23.08-10 × 20.5 and those against the GII strains were 10 × 20.75-10 × 20.25. Preliminary evidence suggested that the antibody titer of the DL39 strain against GI was higher than that against GII. Subsequent cross-neutralization assays with cat serum prepared with the DL39 strain and each strain simultaneously yielded results similar to those described above. In vivo challenge tests revealed that the DL39 strain-immunized cats outperformed the positive controls in all measures. The results of several trials demonstrated that strain DL39 can potentially be used as a vaccine strain. The study attempted to combine the genetic diversity and phylogenetic analysis of FCV with the discovery of potential vaccines, which is crucial for developing highly effective FCV vaccines.

Measuring Neutral Hydrogen Properties in the Interplanetary Medium

Measuring Neutral Hydrogen Properties in the Interplanetary Medium