Small embryoid bodies (EB's) from the OTT-6050 transplantable mouse teratoma, obtained by gravity filtration through a 74 μ mesh, were injected into the right cerebral hemisphere of syngeneic newborn or adult mice of both sexes in order to produce differentiating teratomas after a single passage. In subsequent experiments, two solid tumors resulting from two different EB-implants into the brains of adult hosts were used to initiate sequential tumors and were carried intracerebrally in adult mice for 12 and 18 passages respectively. The animals were sacrificed when signs of increased intracranial pressure developed. Survival times were as follows: single passages in adult mice: mean, 35 days; single passages in neonatal mice: mean, 19 days; sequential passages in adult mice: mean, 25 days. Multipotential stem cells accounted for l/2 to 3/4 of the cens in all tumors. Primitive neural cells, ependymoblastic rosettes, neuroblasts and glia were present in all; stem cells, primitive neural cells and rosettes decreased proportionately as the more differentiated neural populations became prominent. Mature ganglion cells were found only in the sequentially passaged tumors and in tumors maintained for more than one month after a single passage in adult mice. Synapses were noted in the most differentiated areas. Neuroblasts were infrequent in tumors developing in neonatal hosts, and mature ganglion cells were absent. Glial fibrillary acidic protein was present by the 24th day in tumors obtained in adult hosts after single passage and in sequential passages.