Multilayer Assembly Research Articles

The MEMS-Based Electrochemical Seismic Sensor With Integrated Sensitive Electrodes by Adopting Anodic Bonding Technology

Seismic sensors are the key sensitive components in geophysical exploration, and the new-type electrochemical seismic sensors have gradually aroused researchers’ interest for their superior performance in low-frequency domain and large working inclination. In this paper, a method was developed to fabricate the MEMS (micro-electro-mechanical systems) based integrated electrodes for the electrochemical seismic sensors. The proposed integrated electrodes which employed three-layer anodic bonding structure of silicon-glass-silicon served as the substitutes for multilayer manual assembly structures. Compared to previous counterparts, this integrated structure has the advantages of simplified assembly processes of sensitive unit and high consistency as the no requirement of manual alignment. The results shown that the cross-correlation coefficient between two proposed devices was quantified as 0.998 with the sensitivity of 5956 V/(m/s) @1Hz. This electrode is so far the sensitive structure which realize both high sensitivity and high integration in the electrochemical seismic sensors.

Self‐Regenerating of Functional Polymer Surfaces by Triggered Layer Shedding Using a Stimulus‐Responsive Poly(urethane)

AbstractRegeneration of functional surfaces after damage or contamination could extend the life time of devices. Such regeneration can be achieved by layer shedding (like a lizard shedding its skin). In this work, triggered self‐regeneration of functional surfaces by an external stimulus is presented. Polymer multilayer stacks are assembled alternatingly from discrete 20–300 nm thick functional layers and depolymerizable interlayers, which are used as sacrificial layers. The sacrificial layers are depolymerizable poly(benzyl carbamates) end‐capped with 4‐hydroxy‐2‐butanone. Their depolymerization is triggered by alkaline pH, at which the end‐cap is cleaved. This initiates a 1,6‐elimination cascade of the polymer backbone, during which CO2 is released. Thus, the layer shedding is driven synergistically by mass transport and buoyancy forces. Proof‐of‐concept is achieved using poly(styrene) as a model functional layer, and also studied for hydrophilic, antimicrobially active poly(oxanorbornene) layers. The multilayer assembly and disassembly process is monitored by ellipsometry, Fourier transform infrared spectroscopy (FTIR), optical microscopy, and atomic force microscopy. FTIR spectra taken after degradation are confirmed the regeneration of the surface functionality.

GQD embedded bacterial cellulose nanopaper based multi-layered filtration membranes assembly for industrial dye and heavy metal removal in wastewater

GQD embedded bacterial cellulose nanopaper based multi-layered filtration membranes assembly for industrial dye and heavy metal removal in wastewater

A simple fabrication strategy for orientationally accurate twisted heterostructures

Van der Waals (vdW) heterostructure is a type of metamaterial where multiple layers of 2D materials are vertically aligned at controlled misorientation. The relative rotation in between the adjacent layers, or the twist angle between them plays a crucial role in changing the electronic band structure of the superlattice. The assembly of multi-layers of precisely twisted two dimensional layered materials requires knowledge of the atomic structure at the edge of the flake. It may be artificially created by the ‘tear and stack’ process. Otherwise, the crystallographic orientation needs to be determined through invasive processes such as transmission electron microscopy or scanning tunneling microscopy, and via second-harmonic generation (SHG). Here, we demonstrate a simple and elegant transfer protocol using only an optical microscope as a edge identifier tool through which, controlled transfer of twisted homobilayer and heterobilayer transition metal dichalcogenides is performed with close to 100% yield. The fabricated twisted vdW heterostructures have been characterized by SHG, Raman spectroscopy and photoluminiscence spectroscopy, confirming the desired twist angle within ∼0.5° accuracy. The presented method is reliable, quick and prevents the use of invasive tools which is desirable for reproducible device functionalities.

Artificial neural network and regression models for prediction of sewing thread consumption for multilayered fabric assembly at lockstitch 301 seam

Purpose This paper aims to propose the artificial neural network (ANN) and regression models for the estimation of the thread consumption at multilayered seam assembly stitched with lock stitch 301. Design/methodology/approach In the present study, the generalized regression and neural network models are developed by considering the fabric types: woven, nonwoven and multilayer combination thereof, with basic sewing parameters: sewing thread linear density, stitch density, needle count and fabric assembly thickness. The network with feed-forward backpropagation is considered to build the ANN, and the training function trainlm of MATLAB software is used to adjust weight and basic values according to the optimization of Levenberg Marquardt. The performance of networks measured in terms of the mean squared error and the layer output is set according to the sigmoid transfer function. Findings The proposed ANN and regression model are able to predict the thread consumption with more accuracy for multilayered seam assembly. The predictability of thread consumption from available geometrical models, regression models and industrial empirical techniques are compared with proposed linear regression, quadratic regression and neural network models. The proposed quadratic regression model showed a good correlation with practical thread consumption value and more accuracy in prediction with an overall 4.3% error, as compared to other techniques for given multilayer substrates. Further, the developed ANN network showed good accuracy in the prediction of thread consumption. Originality/value The estimation of thread consumed while stitching is the prerequisite of the garment industry for inventory management especially with the introduction of the costly high-performance sewing thread. In practice, different types of fabrics are stitched at multilayer combinations at different locations of the stitched product. The ANN and regression models are developed for multilayered seam assembly of woven and nonwoven fabric blend composition for better prediction of thread consumption.

Nanoscale Bouligand Multilayers: Giant Circular Dichroism of Helical Assemblies of Plasmonic 1D Nano-Objects.

Chirality is found at all length scales in nature, and chiral metasurfaces have recently attracted attention due to their exceptional optical properties and their potential applications. Most of these metasurfaces are fabricated by top-down methods or bottom-up approaches that cannot be tuned in terms of structure and composition. By combining grazing incidence spraying of plasmonic nanowires and nanorods and Layer-by-Layer assembly, we show that nonchiral 1D nano-objects can be assembled into scalable chiral Bouligand nanostructures whose mesoscale anisotropy is controlled with simple macroscopic tools. Such multilayer helical assemblies of linearly oriented nanowires and nanorods display very high circular dichroism up to 13 000 mdeg and giant dissymmetry factors up to g ≈ 0.30 over the entire visible and near-infrared range. The chiroptical properties of the chiral multilayer stack are successfully modeled using a transfer matrix formalism based on the experimentally determined properties of each individual layer. The proposed approach can be extended to much more elaborate architectures and gives access to template-free and enantiomerically pure nanocomposites whose structure can be finely tuned through simple design principles.

Structural insight into host plasma membrane association and assembly of HIV-1 matrix protein

Oligomerization of Pr55Gag is a critical step of the late stage of the HIV life cycle. It has been known that the binding of IP6, an abundant endogenous cyclitol molecule at the MA domain, has been linked to the oligomerization of Pr55Gag. However, the exact binding site of IP6 on MA remains unknown and the structural details of this interaction are missing. Here, we present three high-resolution crystal structures of the MA domain in complex with IP6 molecules to reveal its binding mode. Additionally, extensive Differential Scanning Fluorimetry analysis combined with cryo- and ambient-temperature X-ray crystallography and GNM-based transfer entropy calculations identify the key residues that participate in IP6 binding. Our data provide novel insights about the multilayered HIV-1 virion assembly process that involves the interplay of IP6 with PIP2, a phosphoinositide essential for the binding of Pr55Gag to membrane. IP6 and PIP2 have neighboring alternate binding sites within the same highly basic region (residues 18–33). This indicates that IP6 and PIP2 bindings are not mutually exclusive and may play a key role in coordinating virion particles’ membrane localization. Based on our three different IP6-MA complex crystal structures, we propose a new model that involves IP6 coordination of the oligomerization of outer MA and inner CA domain’s 2D layers during assembly and budding.

Multiscale modelling of graphene sheet and its application in laminated composites

Multiscale modeling strategy development of graphene and its composites is of critical importance in engineering and technological applications. In this work, we systematically developed a chirality preserved coarse-grained graphene model and the corresponding potential, TersoffCG(16–1). The robustness of this potential is verified through structural and mechanical characterization analysis comparison between coarse-grained and all-atomistic models. The Lennard-Jones potential is emphasized to adjust the interlayer properties of the interlayer distance and binding energy. Furthermore, in-plane properties of multilayer graphene assembly tensile and monolayer graphene crack, and out-of-plane properties of nanoindentation are fully analyzed. The copper/graphene nanocomposite tensile test indicated that the coarse-grained graphene model is compatible with the copper matrix. Our efficient and portable coarse-grained graphene model successfully reproduced the architectural parameters, elastic, fracture, and interlayer adhesion properties of graphene sheets compare with the reported experiment and theoretical results, which can serve as an effective strategy to research more hybrid composite systems.

Broadband sound absorbers of multilayered micro-slit panels using Bayesian probabilistic inference

Micro-perforated panel absorbers can typically achieve either visual transparency or broadband absorption, but not both. This paper assesses the potential of Multilayer Micro-Slit panels to maintain both of these characteristics simultaneously. Micro-slit panels are similar to micro-perforated panels, and can similarly achieve high absorption coefficients without fibrous backing materials. The arrangement of slits are better suited to visual transparency than perforated holes because it provides more unobstructed panel per perforated area. However, these types of absorbers are limited to a narrow frequency bandwidth of effective absorption. By combining several panels into a multilayer assembly, broadband absorption becomes possible. The inherent complexity stemming from optimizing the parameters for multiple layers to meet a given design criteria necessitates the use of the Bayesian framework. This probabilistic method rapidly hones in on the best parameters of each individual layer so that the overall composite meets the design goal. Furthermore, Bayesian inference implemented cyclically alongside panel fabrication and testing allows for corrections of fabrication tolerances while assessing visual transparency.

Multilayer assembly of thin-film nanocomposite membranes with enhanced NaCl and antibiotic rejection

Reverse osmosis and nanofiltration by thin-film nanocomposite (TFN) membranes are energy-efficient processes for water purification. However, it is difficult to fabricate high-performance TFN membranes in controllable manner. In this work, the uniform and multilayered polyamide (PA)-graphene oxide (GO) TFN membranes were assembled by a simple and controllable strategy, named as multilayer deposition, which was performed by consecutively spin-coating GO, meta-phenylene diamine (MPD)/piperazine (PIP), and trimesoyl chloride (TMC) solutions on the substrates. The introduction of GO could reduce defects, resulting in formation of smooth and defect-free reverse osmosis/nanofiltration membranes with significantly increased NaCl and antibiotic rejection. For example, the TFN reverse osmosis membrane showed NaCl rejection of 99.1% and water permeance of 1.83 L m−2 h−1 bar−1, accompanied by substantially enhanced antifouling property.

Real-Time NMR Monitoring of Spatially Segregated Enzymatic Reactions in Multilayered Hydrogel Assemblies*.

Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high‐resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi‐layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by monitoring reagent and product concentrations in real‐time. Simultaneous monitoring of an independent enzymatic process (Urs) together with a cooperative process (GOx + Cat) was achieved, with chemical conversion modulation of the GOx + Cat process demonstrated by varying the order in which the hydrogel was assembled.

Real‐Time NMR Monitoring of Spatially Segregated Enzymatic Reactions in Multilayered Hydrogel Assemblies**

AbstractCompartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high‐resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi‐layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by monitoring reagent and product concentrations in real‐time. Simultaneous monitoring of an independent enzymatic process (Urs) together with a cooperative process (GOx + Cat) was achieved, with chemical conversion modulation of the GOx + Cat process demonstrated by varying the order in which the hydrogel was assembled.

Free-Standing Thin Hydrogels: Effects of Composition and pH-Dependent Hydration on Mechanical Properties

We developed an approach to obtain nanothin free-floating poly(methacrylic acid) (PMAA) hydrogel films through the dissolution of a sacrificial SiO2 layer. The hydrogel films were produced by chemical crosslinking of PMAA in PMAA/poly(N-vinylpyrrolidone) (PVPON) hydrogen-bonded precursors assembled through spin-assisted (SA) multilayer assembly. Surface-anchored precursor films and hydrogels were released from silicon templates into an aqueous solution via etching the thick sacrificial SiO2 layer from the templates. We studied the impact of the release method on the surface morphology, thickness, and elasticity of the released (PMAA/PVPON)60 and (PMAA)60 films using atomic force microscopy (AFM) and demonstrated that these properties were affected neither by the film release nor by their following transfer onto Si wafers. We found that 16 h crosslinked (PMAA)60 hydrogels and their more swollen 8 h crosslinked counterparts (76% hydration at pH = 5) produced free-floating films with good mechanical integrity and strength. The elastic moduli of a 16 h crosslinked (PMAA)60 film decreased from 1.9 ± 0.1 GPa in the dry state to 77 ± 13 MPa at pH = 5 and then to 14 ± 3 MPa at pH = 6.5. We also showed that incorporating Zr(IV) into twice thinner (PMAA)30 hydrogels (∼60 nm dry thickness) improved film mechanical robustness and allowed their successful release and transfer onto Si wafers. In contrast, Zr-free (PMAA)30 were less tolerant to the release/transfer procedure due to mechanical fragility. The temporary coordination links between Zr(IV) and the hydrogel could be removed from the network by an EDTA chelator. The lift-off approach developed here is simple, versatile, and applicable to a wide range of polymer films. The free-floating hydrogels obtained by this method can be used as transferrable platforms to develop lab-on-a-chip systems, nanocomposite pressure sensing platforms, rapid optical biosensors, and responsive platforms for regulating cell adhesion and more effective cell sheet recoveries.

Polyelectrolyte Multilayer Films Based on Natural Polymers: From Fundamentals to Bio-Applications.

Natural polymers are of great interest in the biomedical field due to their intrinsic properties such as biodegradability, biocompatibility, and non-toxicity. Layer-by-layer (LbL) assembly of natural polymers is a versatile, simple, efficient, reproducible, and flexible bottom-up technique for the development of nanostructured materials in a controlled manner. The multiple morphological and structural advantages of LbL compared to traditional coating methods (i.e., precise control over the thickness and compositions at the nanoscale, simplicity, versatility, suitability, and flexibility to coat surfaces with irregular shapes and sizes), make LbL one of the most useful techniques for building up advanced multilayer polymer structures for application in several fields, e.g., biomedicine, energy, and optics. This review article collects the main advances concerning multilayer assembly of natural polymers employing the most used LbL techniques (i.e., dipping, spray, and spin coating) leading to multilayer polymer structures and the influence of several variables (i.e., pH, molar mass, and method of preparation) in this LbL assembly process. Finally, the employment of these multilayer biopolymer films as platforms for tissue engineering, drug delivery, and thermal therapies will be discussed.

Cofiring of LTCC multilayer assemblies with integrated NTC thermistor temperature sensor layers

We have studied the integration of Ni–Co–Zn–Mn spinel NTC thermistor layers into LTCC multilayer modules. Sintering of the cubic spinel Ni0.5Co0.5Zn0.75Mn1.25O4 with addition of 3 wt% Bi2O3 additive at 900 °C resulted in samples with 96% density and with good electrical performance. Thermistor spinel tapes, fabricated using doctor blade tape casting, were stacked with layers of a commercially available low-k dielectric LTCC tape, and cofired at 900 °C. Free or constrained cofiring did not give crack-free multilayer samples. Thermistor layers were then integrated combining the commercial LTCC tape with a Zn titanate-based tape in one multilayer laminate, and error-free multilayer samples with a room-temperature resistivity of ρ298 = (2,9 ± 0.08) kΩcm and a thermistor constant of B = (4280 ± 200) K were obtained. This demonstrates the possibility of cofiring of functional ceramic and low-k LTCC tapes and shows the potential of NTC spinel thermistor layers as temperature sensors integrated in complex LTCC multilayer architectures.

Preparation the antibacterial coatings based on natural mineral materials

The purpose of the study is to obtain nanofilms based on clay minerals and to study the antibacterial activity of the obtained nanocomposite coatings. Diatomite and kaolin were pretreated with sulfuric acid. Silicon plates were used as model substrates. For the first time, multilayers of the composition diatomite/PAA and kaolin/PAA were obtained by multilayer assembly. In the multi-layer, the antiseptic chlorhexidine was introduced, which plays the role of an antibacterial agent. Scanning electron microscopy (SEM) determined the distribution and thickness of the obtained multi-layers, the elemental composition of nanofilms was determined by the SEM-EDX method, the specific surface of natural and sulfuric acid-modified diatomite and kaolin was examined by the BET method, and the wetting angle was also determined by the lying drop method. Obtaining coatings on the surface of implantable systems is one of the promising areas in modern medicine. In this regard, the production of nanocovers with antibacterial properties is an urgent issue in the fight against infectious diseases associated with bacterial growth. One of the modern methods for producing nanofilms that are currently used is the Layer by Layer (LBL) method. Nanofilms obtained by this method have found application in tissue engineering and dressings. The peculiarity of this study is the use of clay minerals such as diatomite of the Mugodzharsky deposit and kaolin of the Alekseevsky deposit, which are concentrated in the west and north of Kazakhstan. The obtained multilayers were used as carriers for chlorhexidine and their antibacterial activity was studied. Nanofilms of diatomite/PAA/chlorhexidine and kaolin/PAA/chlorhexidine were tested against the E. Coli gram-negative bacterium. Thus, the obtained nanocomposite coatings based on kaolin and diatomite can be potential carriers for drugs.

(Invited) Transparent and Flexible PEDOT:PSS/ITO/Ag/ITO Microelectrodes for Electrophysiology Recording

High-resolution microelectrodes made of transparent conducting materials are advantageous for electrophysiology recordings when used in combination with optogenetic stimulation or optical imaging. Existing transparent electrodes made of indium tin oxide (ITO), ultrathin metal, graphene, poly-(3, 4-ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT:PSS), gold nanomesh, or silver nanowire network present tradeoffs with optical transmittance, resistivity, mechanical flexibility, and biocompatibility. In this work, we designed and constructed a flexible, fully transparent microelectrode array using a PEDOT:PSS/ITO/Ag/ITO multilayer assembly. Nanometer-thick Ag provides high electrical conductivity while being transparent over a broad spectrum. ITO offers excellent transparency as well as chemical and biological stability to protect Ag from corrosion in the biological environments. PEDOT:PSS further reduces electrochemical impedance by increasing the active surface area with the same electrode size. The thickness of each layer of the multilayer assembly was optimized at a specific wavelength using admittance loci analysis to minimize the reflectance at the inhomogeneous material interfaces. As a proof of concept, an electrode array was designed to have two sub panels, each of which consisted of 16 microelectrodes on a 3x3 mm2 area, with various electrode diameters of 25 µm to 50 µm. The fabrication of such an array started from Parylene C deposition on a 3-inch silicon wafer. Photoresist (PR) was spun on the substrate and photolithographically patterned as a sacrificial mask. After that, ITO (20 nm), Ag (9.5 nm) and ITO (24 nm) thin films were sputtered consecutively in a radio-frequency (RF) magnetron sputtering system (Denton Explorer-14, Denton Vacuum, Inc), and then patterned using the liftoff process to form microelectrodes, interconnection wires, and contact pads. Next 2 μm Parylene C was deposited on the substrate as an insulating layer and selectively etched using oxygen plasma dry etching (RIE-1701 plasma system, Nordson March, Inc) to expose the recording sites and contact pads. After that, another PR mask was patterned to expose only the microelectrode sites followed by coating diluted 0.55% PEDOT:PSS. Finally, PR was rinsed off with acetone, IPA and DI water to remove unwanted PEDOT:PSS, leaving PEDOT:PSS only on top of the ITO-Ag-ITO microelectrodes.The optical, electrical, electrochemical, and mechanical properties of the PEDOT:PSS-ITO-Ag-ITO multilayer assembly was characterized using various experimental methods. In particular, a Filmetrics thin film analyzer was utilized to measure the transmittance of thin films in a wavelength range of 300 nm - 800 nm (F20-UVX, Filmetrics, Inc) and a four-point probe (SRM-232, Bridge Technology, Inc) analyzer was used to measure the sheet resistances of the samples. Compared to a single ITO film of the same thickness, the PEDOT:PSS/ITO/Ag/ITO assembly exhibits a combination of high broad-band transmission (>80%) and significantly improved conductivity. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) analyses demonstrated the significantly enhanced electrical impedance of the multilayer film by 91.85%. The electrochemical impedance of the films remained stable after 12 weeks of storage in room temperature air, with less than 12% change. Meanwhile, peel-off tests verified the excellent adhesion of the ITO-Ag-ITO assembly on the Parylene C substrate, and bending test results demonstrated the mechanical flexibility of the combined thin films over a large surface area. In addition, the noise performance of the transparent microelectrodes was analyzed under exposure to optical illumination of various wavelengths, confirming the negligible photon-induced artifacts suitable for electrophysiology recording in combination with optical imaging or stimulation. The optical transparency of the microelectrode array was further demonstrated under both the fluorescent and bright-field illumination conditions. Finally, the ability to use the transparent microelectrodes for recording neurophysiology and electrocardiography signals was demonstrated in vivo using rat models or in vitro using heart organoids.

Fire blanket and intumescent coating materials for failure resistance

There is major fire safety concern about failure propagation of thermal runaway in multicell lithium-ion batteries. This article overviews the passive fire-protection approach based on thermal insulation by intumescent coating materials and fire blankets for viable failure resistance. The intumescent coating will expand (up to 100× on heating) to form a thick, porous char layer and act as a thermal barrier to insulate the substrate. It is also used to seal around openings in a wall or floor to impede the spread of fire and smoke. High-temperature fire blankets are made of noncombustible or flame-resistant fabric materials (e.g., aramids, fiberglass, amorphous silica, preoxidized carbon, and mineral fibers). Both working intumescent coating and fire blankets can block a significant portion (typically 60 to 90%) of the incident heat. Impact-resistant high-strength fabrics, in either soft or rigid forms, can also be used as parts of multilayer protection assembly. Thus, multilayer assemblies can be used for various commercial products, including passive and active fire-protection blankets, battery-cell partitions, confinement bags and containers, packaging materials, and personal protective equipment.

Adsorption Properties and Electron-transfer Rates of a Redox Probe at Different Interfaces of an Immunoassay Assembled on an Electro-active Photonic Platform.

Physical and chemical properties of a redox protein adsorbed to different interfaces of a multilayer immunoassay assembly were studied using a single-mode, electro-active, integrated optical waveguide (SM-EA-IOW) platform. For each interface of the immunoassay assembly (indium tin oxide, 3-aminopropyl triethoxysilane, recombinant protein G, antibody, and bovine serum albumin) the surface density, the adsorption kinetics, and the electron-transfer rate of bound species of the redox-active cytochrome c (Cyt-C) protein were accurately quantified at very low surface concentrations of redox species (from 0.4 to 4% of a full monolayer) using a highly sensitive optical impedance spectroscopy (OIS) technique based on measurements obtained with the SM-EA-IOW platform. The technique is shown here to provide quantitative insights into an important immunoassay assembly for characterization and understanding of the mechanisms of electron transfer rate, the affinity strength of molecular binding, and the associated bio-selectivity. Such methodology and acquired knowledge are crucial for the development of novel and advanced immuno-biosensors.

Transport and assembling microparticles via Marangoni flows in heating and cooling modes

The processes of the particles transfer in liquid media and the creation of structures of required configurations on substrates are of great importance in such areas as materials science, coatings, optoelectronics and biomedical researches. To control of the transfer of large ensembles of particles and dynamically transform of the particle aggregates the precise and flexible methods are strongly required. In this work, we propose a method for manipulation of microparticles in volatile liquid layers hundreds of microns thick that relies on the control of Marangoni flows by changing a sign of the temperature gradient in the liquid by the local action of the heat source and/or the heat sink. We have clearly demonstrated the applicability of the method to perform of wide range of manipulations with the particle ensembles: particles assembling in the circular patterns on a substrate when heating is applied, transfer of particles away from a heat sink when the substrate is cooled, and creation of ring-like patterns by changing the temperature gradient sign during particle assembling process. We have also studied the influence of the liquid layer thickness on the particle transfer and the size of the resulting pattern. It was found out that when heating the final pattern area on substrate decreases with the layer thickness, while the time of the pattern formation increases with the layer thickness. In the cooling mode, the final cleaned area decreases with the number of particles but the dependence on the layer thickness is ambiguous. The characteristic time decreases with an increase in the number of particles and slightly increases with the layer thickness. It was also revealed that the increase in the layer thickness makes it possible to create the multilayer dense circular assemblies of particles. The proposed method is simple for implementation, flexible and can be applied for manipulation with particles of any physicochemical properties and shapes. The method is applicable for controlling both a single microparticle and a large group of particles.