Morphology Of AgNPs Research Articles

Two-step process for synthesizing wheat-like silver nanoparticles by using facile wet-chemical method

Wheat-like silver nanoparticles (AgNPs) have been facilely synthesized by using two-step reduction method. The initial AgNPs are reduced from silver nitrate in the first step. Effects of ethyl cellulose concentration on the structure and morphology evolution of AgNPs are systematically investigated in the second step. With increasing the concentrations of ethyl cellulose, the AgNPs evolves from cauliflower-like particles to wheat-like species via a transitional shape of slender particles. The trade-off between oxidation of nitric acid and reduction of L-ascorbic acid under the induction of ethyl cellulose dispersant modifies the morphology of AgNPs, contributing to forming various shapes. The phase structure of synthesized AgNPs remains crystalline and independent of ethyl cellulose concentration. Our works indicate that such a wet-chemical method provides an easy-to-do approach for preparing AgNPs with specific shapes.

Karakter Fisik dan Aktivitas Antibakteri Nanopartikel Perak Hasil Green Synthesis Menggunakan Ekstrak Air Daun Sendok (Plantago major L.)

Silver nanoparticles have been studied for its application in wound healing therapy, while aqueous extract of Plantago major L has been studied also for its antibacterial and wound healing activity. This study aimed to obtain silver nanoparticles (AgNPs) through a green synthesis pathway using aqueous extract of Plantago major L. The initial study was performed to optimize the three concentration points of Plantago major L. aqueous extract, namely 0.125%, 0.25% and 0.5% and three synthetic temperature points (60 °C, 70 °C, and 80 °C). The formation of AgNPs were confirmed using a UV-Vis spectrophotometer which shows a peak at a wavelength of 445 nm. The obtained silver nanoparticles were then characterized using Dynamic Light Scattering (DLS Nano), Scanning Electron Microscope (SEM) dan X-Ray Diffraction (XRD). The study showed that the extract concentration of 0.25% with an optimum temperature of 70 °C was the optimal combination to produce the best AgNPs, based on the organoleptic parameters (as the color of silver was produced), the highest yield with weight of 9.13 mg and particle size of 129.20 nm with Polydispersity Index (PDI) of 0.25. The morphology of AgNPs showed the spherical shape and the crystallization of AgNPs showed a diffractogram pattern similar to the silver standard using X-Ray Diffraction (XRD). Antibacterial activity of AgNPs was tested against three bacterial strains, namely Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa and was measured with diffusion method using paper disc. The antibacterial activity testing showed that AgNPS has better antibacterial activity than silver nitrate and is equivalent to its comparative antibiotics, gentamicin sulfate for gram-negative bacteria and Chloramphenicol for gram-positive bacteria.

Meio dispersivo na síntese eficiente de nanopartículas de prata para aplicação em tecidos antimicrobianos

As nanopartículas de prata têm sido cada vez mais utilizadas em aplicações industriais há muito tempo. Os métodos químicos de síntese de nanopartículas de prata podem ser tóxicos e caros. Portanto, muitos estudos têm surgido para criar rotas de síntese ecologicamente corretas que forneçam boa dispersão e estabilidade no meio extrativo. Espécies vegetais são investigadas para a descoberta de um novo meio dispersivo para nanopartículas de prata (AgNPs) sintetizadas por via verde. As AgNPs estão dispersas em extrato vegetal, por plantas disponíveis no Brasil. Neste trabalho foram utilizadas folhas de Melissa Officinalis como meio dispersivo das nanopartículas. Através das técnicas de caracterização utilizadas foi possível observar as modificações superficiais com a presença de nanoestruturas bem dispersas, que são fundamentais para a confecção de tecidos inteligentes com ação antimicrobiana. Assim foi possível por meio de cada rota de síntese avaliar a dispersão e morfologia das AgNPs em diferentes meios dispersivos de Melissa Officinalis, concluindo-se que o mesmo é muito eficiente para estabilização das AgNPs.

Synthesis and Characterization Of Silver Nanoparticles Using Water Extract of Sarang Semut (Myrmecodia pendans) For Blood Glucose Sensors

The principles of green chemistry in nanotechnology is one of the key issues in nanoscience research. Biosynthesis of nanoparticles using plant extract is an eco- friendly approach which eliminates the need for using physical and chemical techniques. A green synthetic route for the production of stable silver nanoparticles (AgNPs) by using aqueous silver nitrate as metal precursor and Myrmecodia pendans extract as bioreductor and PVA as stabilizer is being reported for the first time. In this case, the synthesis of silver nanoparticles (AgNPs) was carried out by reduction method. The bio-reduction and stabilization of so formed silver nanoparticles was monitored by UV-Vis spectrophotometry. Maximum absorption of AgNPs using Polyvinyl Alcohol (PVA) was at wavelength 408.50 nm, whereas that of AgNPs without using PVA was at wavelength of 408 nm. SEM images showed that the morphology of AgNPs was globular. An average size of silver nanoparticles using PVA and without using PVA was 78.3 and 76.1 nm, respectively. The results of X-Ray Diffraction (XRD) displayed the same peak pattern with that of the standard silver metal showing that the resultant particles are silver nanoparticles having FCC structure. Analysis of glucose levels in blood using silver nanoparticles based sensors showed glucose concentration of 71.71 mg / dL.

Biosynthesized silver nanoparticles for inhibition of antibacterial resistance and biofilm formation of methicillin-resistant coagulase negative Staphylococci

The ability of a natural stabilizing and reducing agent on the synthesis of silver nanoparticles (Ag NPs) was explored using a rapid and single-pot biological reduction method using Nocardiopsis sp. GRG1 (KT235640) biomass. The UV–visible spectral analysis of Ag NPs was found to show a maximum absorption peak located at a wavelength position of ∼422 nm for initial conformation. The major peaks in the XRD pattern were found to be in excellent agreement with the standard values of metallic Ag NPs. No other peaks of impurity phases were observed. The morphology of Ag NPs was confirmed through TEM observation, demonstrating that the particle size distribution of Ag NPs entrenched in spherical particles is in a range between 20 and 50 nm. AFM analysis further supported the nanosized morphology of the synthesized Ag NPs and allowed quantifying the Ag NPs surface roughness. The synthesized Ag NPs showed significant antibacterial and antibiofilm activity against biofilm positive methicillin-resistant coagulase negative Staphylococci (MR-CoNS), which were isolated from urinary tract infection as determined by spectroscopic methods in the concentration range of 5–60 µg/ml. The inhibition of biofilm formation with coloring stain was morphologically imaged by confocal laser scanning microscopy (CLSM). Morphological alteration of treated bacteria was observed by SEM analysis. The results clearly indicate that these biologically synthesized Ag NPs could provide a safer alternative to conventional antibiofilm agents against uropathogen of MR-CoNS.

Green synthesis, size control, and antibacterial activity of silver nanoparticles on chitosan films

Silver nanoparticles (AgNPs) synthesized on the surface of chitosan (CS) films using ultraviolet (UV) and natural light irradiation reduction methods were studied. Field-emission scanning electron microscopy revealed that the diameter of some AgNPs synthesized by the UV irradiation reduction method lay in the range of 5–15 nm. These AgNPs were isolated and dispersed on the surface of CS film. Meanwhile, some AgNPs aggregated to form larger cubic and irregular AgNPs with average size of 50–200 nm. Compared with those synthesized via the UV irradiation reduction method, AgNPs synthesized by the natural light irradiation reduction method were found to be much more individually isolated and uniformly dispersed on the CS film surface. The AgNPs synthesized using the natural light irradiation reduction method had spherical shape with average size of 10–30 nm. Thus, the size and morphology of AgNPs on the CS films (CS-AgNPs films) can be controlled by choosing an appropriate irradiation reduction method. The results of antibacterial testing illustrated that the CS-AgNPs films synthesized by both the ultraviolet and natural light irradiation reduction methods exhibited strong antibacterial activity against Escherichia coli.

Synthesis and Characterization of Bioinspired Silver Nanoparticles by Aqueous Leaf Extract of Indigofera cassioides: Evaluation of Antimicrobial and Cytotoxic Activity

The green synthesis of silver nanoparticles (AgNPs) has increasingly promising due to their potential applications in nanomedicine. For the first time, silver nanoparticles (AgNPs) were synthesized from aqueous leaf extract of Indigofera cassioides. The AgNPs were characterized by UV-Vis spectroscopy and the characteristic surface plasmon resonance peak was observed at 414 nm. The Fourier transform infrared spectroscopy (FT-IR) revealed the phytochemicals in the plant extract which assisted bioreduction, capping and stabilizing agent. Morphology of AgNPs was characterized by transmission electron microscopy (TEM) and they were spherical shape with average particle size as 20 nm. The face centered cubic (FCC) crystalline structure of the AGNPs was confirmed by X-ray diffraction. Dynamic light scattering (DLS) and zeta potential analysis revealed that the average hydrodynamic diameter of the AGNPs as 80 nm and the negative zeta potential value of -36.6 mV indicates good stability. Antimicrobial studies disclosed that the biosynthesized AgNPs possess strong activity against pathogenic bacteria. The cytotoxicity study is concealed that the AgNPs exhibit selective cytotoxicity against the tested cell lines and a pronounced activity was observed against human breast cancer cells (MCF-7). The present study concludes that for the biosynthesized AgNPs could be used as an antimicrobial and anticancer agent.

Rosa damascena waste mediated synthesis of silver nanoparticles: Characteristics and application for an electrochemical sensing of hydrogen peroxide and vanillin

Silver nanoparticles (AgNPs) were synthesized by reduction of Ag+ with four extracts of Rosa damascena waste: post-distillation water (PW), water extract (WE), 30% (30 EE) and 70% (70 EE) ethanol extracts. The ethanolic extracts were rich in polyphenols and particularly catechin and epicatechin (1388.01 ± 2.27 and 677.47 ± 2.03 μgmL−1, respectively) while the water extracts were rich in reducing sugars, proteins and pectic substances (for WE 254.21 ± 1.26, 296.89 ± 2.01 and 242.14 ± 1.38 μgmL−1, and for PW 185.72 ± 1.47, 298.30 ± 1.57 and 181.78 ± 1.39 μgmL−1, respectively). The morphology of AgNPs was characterized by transmission electron microscopy and the calculated average size of the AgNPs synthesized using WE and PW was 25.8 ± 11.5 nm and 11.5 ± 8.4 nm, respectively. The X-ray diffraction analysis suggested presence of silver phase which crystallized in a face centered cubic space group Fm-3m with unit cell parameter 4.085 (2) Å and mean coherent domain size 10.9 (3)nm. Biosynthesized AgNPs were deposited onto a spectroscopic graphite electrode (GE), applying two different procedures, and stabilized using chitosan to build modified electrodes-catalysts. The electrochemical performances of the modified electrodes AgNPs/GE and AgNPs/CS/GE were studied by means of cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry at pH 7.0 and their applicability for amperometric detection of H2O2 and vanillin was investigated. The developed electrodes exhibited a sensitive and reproducible response for quantitative determination of H2O2 at applied potentials of −0.2, −0.25 and −0.3 V (vs. Ag/AgCl, 3 M KCl). Constant potential amperometry studies with AgNPs/CS/GE showed sensitive response to H2O2 (115.2 μA mM−1 cm−2) up to 6.6 mM at −0.3 V, and to vanillin (56.8 μA μM−1 cm−2) up to 0.5 mM with detection limit of 8.4 μM at 0.58 V. The results obtained from the quantitative electrochemical measurements of H2O2 and vanillin offer potential applications of the graphite electrodes modified with the biosynthesized AgNPs in electrochemical sensor devices for monitoring of these analytes.

Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Kluwak Pangium edule Reinw Sebagai Bioreduktor dan Uji Aktivitasnya Sebagai Antioksidan

Silver nanoparticles have been synthesized using kluwak leaf extract (Pangium edule Reinw) as bioreductor and antioxidant activity assay. The nanoparticles formed were monitored by observing UV-Vis absorption and characterized by using FTIR, PSA, XRD and SEM instruments. The result of functional group characterization with FTIR show that the functional groups OH, C = O, C-O and CH2 act as Ag+ reducing agent. The size of silver nanoparticles was determined by using Particle Size Analyzer (PSA) and the result show average particle size distribution of 93.2 nm. Morphology of AgNp were observed by Scanning Electron Microscope (SEM) and X-Ray Difraction (XRD) analysis show result of 51,78 nm. The antioxidant activity was shown by in kluwak leaf extract and silver nanoparticles with IC50 values respectively 831,33 ppm dan 1493,09 ppm.

Bio Synthesis and Characterization of Silver Nanoparticles by Leaf Extracts of Moringa Oleifere Leaf, Azardica Indica (Neem) Leaf, Bamboo Leaf, and their Antibacterial Activity

Abstract Silver nanoparticles (AgNps) have a number of applications in medical domain. Since these nanoparticles have been used for infection, prevention in the Medical study. The plant leaves extracts are used in biosynthesis of nanostructure materials can be non-toxic & cost effective; it may be more relevant to reduce their size. A Green rapid biogenic synthesis of silver nanoparticles using moringa oleifere leaf extract to compare with A.indica leaf & bamboo leaf aqueous extracts was demonstrated in some of the cases. The obtained AgNps were synthesized using M. oleifere leaf extract have been characterized by X ray diffraction (XRD) analysis shows that particles average size 28 .45 nanometer, Fourier transform infrared spectroscopy (FTIR) studies suggests that the present of function group , Energy dispersive X-ray spectroscopy (EDS) results showed the compositional mass ratio, UV-visible spectrum reveals that resonance band at ∼ 420 nm. Scanning electron microscope (SEM) shows that the morphology of AgNps can be controlled by using different leaf extracts. The bacterial activity was evaluated using E-Coli and S.Aureus.from the disc diffusion results, the synthesized AgNps shows an excellent antibacterial property and compared to the different leaves extracts.

Temperature-dependent green biosynthesis and characterization of silver nanoparticles using balloon flower plants and their antibacterial potential

Environmentally benign silver nanoparticles (Ag NPs) were synthesized at different temperatures using leaf extract of Platycodon grandiflorum. The apparent UV–Vis spectrum maxima clearly displayed plasmon resonance bands at 442 and 457 nm for nanoparticles synthesized at 37 °C and 50 °C, respectively. The morphology of Ag NPs were investigated by FESEM, FETEM and AFM analysis and the results showed the formation of uniform spherical nanoparticles with average sizes of 19 and 21 nm for Ag NPs produced at 37 °C and 50 °C, respectively, with slightly larger nanoparticles formed at 50 °C owing to agglomeration. Further characterization by XRD, XPS, and FTIR confirmed their crystal nature, while stability was confirmed by zeta potential analysis. The strong XRD diffraction pattern confirmed the face-central cubic crystalline nature of the nanoparticles. Moreover, XPS revealed Ag 3d doublet (3d5/2 and 3d3/2) with C1s peaks of the catalysts that were turned into CH (283.68) CC, CC (284.16 eV), CO (285.34 eV), and CO (288.55 eV). The O1s spectra were also exhibited with binding energy at 530.94, 531.96 and 532.53 for AgO, CO and CO, respectively, and FTIR confirmed the peaks of plant extract appeared on Ag NPs. Overall, Ag NPs synthesized at 50 °C had good shape and structure with a high stability (−5.23). The nanoparticles also exhibited antibacterial activity against Escherichia coli and Bacillus subtilis. The biosynthesized Ag NPs have the potential for use in various applications, particularly in the field of biomedicine.

Comparative study of antifungal activity of silver and gold nanoparticles synthesized by facile chemical approach

Colloidal solutions of silver nanoparticles (Ag-NPs) and gold nanoparticles (Au-NPs) have been proved to be suitable antimicrobial agents. In this study, Ag-NPs (yellow color) and Au-NPs (wine red color) have been produced by chemical reduction method using silver nitrate and chloroauric acid as metal precursors with sodium borohydride as reducing agent for Ag-NPs and tannic acid as reducing agent for Au-NPs and trisodium citrate as a stabilizing agent in both cases. UV–vis spectroscopy graph shows the adsorption for Ag-NPs and Au-NPs to be 440 nm and 520 nm respectively. X-ray diffraction (XRD) analysis showed the highly crystalline nature of Ag-NPs and Au-NPs with (111), (200), (220), (311) orientations. TEM and SEM reveal the uniform spherical morphology of Ag-NPs and Au-NPs with the presence of elemental Ag and Au metal analysed by EDAX. SAD confirms the orientation of Ag-NPs and Au-NPs with XRD analysis. The average particle size of Ag-NPs and Au-NPs was found to be 25 nm and 34 nm respectively investigated by DLS. The antifungal activity of Ag-NPs and Au-NPs on fungal cells, i.e., Candida Albicans and S.Cerevesiae showed diminished fungal growth by developing inhibition zones. The comparative studies demonstrate that Ag-NPs are more active than Au-NPs in diminishing fungus.

Effects of ionic strength on physicochemical properties and toxicity of silver nanoparticles

In the environment, silver nanoparticles (AgNPs) undergo a number of potential transformations, such as aggregation, dissolution, and redox reactions. However, the transformation in ionic strength condition, as well as their related toxicity was not quite clear, especially in the in vivo system. In the present study, we comprehensively evaluated three different characteristics (ddH2O, EPA water and K+ medium (KM)) mediated changes in the physical morphology of AgNPs and the alteration of the toxicity to Caenorhabditis elegans (C. elegans). Our results showed besides the changes of AgNPs behavior such as the transformation of morphological, with the transmission electron microscopy we found for the first time that smaller nanoparticles (<5 nm) appeared around the pristine AgNPs after incubation in EPA or KM for 5 days. Together with these changes, the toxicity of AgNPs to C. elegans changed significantly, showing that a higher ionic strength medium resulted in greater toxicity to C. elegans, as measured by germ cell apoptosis, brood size and lifespan. More importantly, our results indicated that the higher toxicity of AgNPs to C. elegans reproduction was probably related to the appearance of the smaller-size AgNPs in higher ionic strength media. These findings highlight that toxicity assessments for the release of nanomaterial to the environment need to be improved to assess environmental safety more accurately.

Green bio-inspired synthesis, characterization and activity of silver nanoparticle forms of Centaurea virgata Lam. and the isolated flavonoid eupatorin

AbstractA green synthesis method of silver nanoparticles (AgNPs) usingCentaurea virgataLam. extract and the isolated compound eupatorin was investigated in this study. Ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM)/energy-dispersive X-ray (EDX) spectroscopy, thermal gravimetric analysis, X-ray diffraction analysis and zeta potential were used for characterization of AgNPs. The UV-Vis spectrum exhibited a characteristic absorption band at 420 nm for monodisperse nanoparticles. FTIR measurements also proved the formation. X-ray diffraction patterns showed peaks at (110) and (112), which are characteristic for hexagonal crystals and also showed peaks at (111), (200) and (240), which are characteristic for orthorhombic crystals. The TEM images of AgNPs show that the morphology of AgNPs was predominantly spherical. Obtained AgNPs were highly stable according to the zeta potential values. The nitric oxide scavenging activity, which is also related to anticancer activity, of AgNPs was evaluated. It can be concluded thatC. virgataLam. extract and eupatorin can be used as a reducing agent for potential antioxidant AgNP formation.

Silver nanoparticle functionalized CS-g-(CA-MA-PZA) carrier for sustainable anti-tuberculosis drug delivery

Recently, drug functionalized biodegradable polymers have been appreciated to be imperative to fabricate multi-drug delivery nanosystems for sustainable drug release. In this work, amphiphilic chitosan–grafted-(cetyl alcohol-maleic anhydride-pyrazinamide) (CS-g-(CA-MA-PZA)) was synthesized by multi-step reactions. The incorporation of rifampicin (RF) and entrapment of silver nanoparticles (Ag NPs) on CS-g-(CA-MA-PZA) polymer was carried out by dialysis technique. From the FT-IR experiment, the polymer modification, incorporation of drugs and the entrapment of Ag NPs on micelles were confirmed. The surface morphology of Ag NPs, polymeric system and drug loaded micelles was described by SEM, TEM and AFM techniques. In addition, the controlled release behaviour of CS-g-(CA-MA-PZA) micelles was studied by UV–Vis spectroscopy. In vitro cell viability, cell apoptosis and cellular uptake experiments shows that multi-drug delivery system could enhance the biocompatibility and higher the cytotoxicity effect on the cells. Since the prepared amphiphilic polymeric micelles exhibit spotty features and the system is a promising strategy for a novel candidate for immediate therapeutically effects for alveolar macrophages.

Comparative catalytic and bacteriostatic properties of silver nanoparticles biosynthesized using three kinds of polysaccharide

Silver nanoparticles (Ag NPs) were biosynthesized using konjac glucomannan (KGM), κ-carrageenan (CRG) and inulin as reducing and capping agent through one-spot method. The structure and morphology of Ag NPs were investigated using UV-visible spectroscopy, transmission electron microscopy, Fourier transform infrared and X-ray powder diffraction. The catalysis and antibacterial properties were also evaluated. Results showed that Ag NPs fabrication was influenced by the incubation temperature and polysaccharide category. CRG displayed a stronger reduction of Ag+ than KGM, and the lowest was inulin. A High incubation temperature promoted the reduction of Ag NPs. As for Ag100 NPs, the kapp of Ag NPs based on CRG, inulin, and KGM were 5.80×10-2 s-1, 2.14×10-2 s-1, and 2.16×10-2 s-1 respectively. Bacteriostatic test showed excellent antimicrobial activity of Ag NPs for Escherichia coli and Staphylococcus aureus. Thus, natural polysaccharide could be effective reducing and stabilizing agents for Ag NPs biosynthesis. The excellent antibacterial and catalytic properties may facilitate their use in medical, food, and biological applications.

Biosynthesis of silver nanoparticles in collagen gel improves their medical use in periodontitis treatment

ABSTRACTSilver nanoparticles (AgNP) suspensions were biosynthesized by silver ions reduction in the presence of collagen, a nontoxic, organic polymer, intending to improve their medical use in periodontitis treatment. Spectrophotometric measurements showed a time- and concentration-dependent increase of AgNP formation in each suspension variant. Transmission electron microscopy revealed spherical morphology of AgNP in collagen and their mean diameter size was around 30 nm. The particle size distribution and zeta potential values of AgNP in collagen were determined by dynamic light scattering measurements. The surface charge of AgNP in collagen was positive, while commercial AgNP stabilized in citrate had negative surface charge. In vitro cytotoxicity testing of AgNP in collagen showed that they were biocompatible with human gingival fibroblasts in a wider range of concentrations than commercial nanoparticles. The antibacterial activity of AgNP in collagen against two pathogenic strains present in the periodontal pocket was dose-dependent and higher than that of AgNP in citrate. All these results demonstrated that AgNP prepared in collagen gel had improved properties, like small diameter, positive surface charge, high biocompatibility in human gingival fibroblasts, efficiency against bacterial growth and, thus, better therapeutic potential in periodontal disease treatment.

An improved green synthesis method and Escherichia coli antibacterial activity of silver nanoparticles

Silver nanoparticles (Ag NPs) were synthesized by an improved green synthesis method via a photo-reduction process using low-power UV light in the presence of poly (vinyl pyrrolidone) (PVP) as the surface stabilizer. The effective synthesis process was achieved by optimized synthesis parameters such as C2H5OH: H2O ratio, AgNO3: PVP ratio, pH value, and reducing time. The formation of Ag NPs was identified by Ultraviolet-visible (UV–vis) absorption spectra, X-ray diffraction pattern (XRD) and Fourier transform infrared spectroscopy (FTIR) spectra. Ag NPs were crystallized according to (111), (200), and (220) planes of the face-centered cubic. The transmission electron microscopy (TEM) image showed that the morphology of Ag NPs was uniform spherical with the average particle size of 16 ± 2 nm. The results of XRD pattern, TEM image, and dynamic light scattering (DLS) analysis proved that Ag crystals with uniform size were formed after the reduction process. The mechanism of the formation of Ag NPs was proposed and confirmed by FTIR spectra. The antibacterial activity of Ag NPs against Escherichia coli (E. coli) was tested and approximately 100% of E. coli was eliminated by Ag NPs 35 ppm. In the future, this study can become a new process for the application of Ag NPs as an antibiotic in the industrial scale.

Catalytic and antibacterial properties of silver nanoparticles green biosynthesized using soluble green tea powder

Silver nanoparticles (Ag NPs) were green fabricated using soluble green tea powder (SGTP) as stabilizer and reducing agent. The properties and morphology of Ag NPs were investigated through UV–visible spectroscopy, field emission transmission electron microscope (FE-TEM) and fourier transform infrared (FT-IR). The spectroscopy showed surface plasmon resonance around at 420 nm revealing the synthesis of Ag NPs. FE-TEM results confirmed that the Ag NPs are spherical and face-centered cubic structure. FT-IR spectroscopy identified the role of various functional groups in the nanoparticle synthesis. The one spot biosynthesized Ag NPs showed favourable antibacterial properties on Escherichia coli and Staphyloccocus aureus, and excellent catalytic reduction of 4-nitrophenol. This work provided a feasible, green method to fabricate Ag NPs with promising photocatalytic and antimicrobial activities.

Silver Nanoparticles Synthesized Using Wild Mushroom Show Potential Antimicrobial Activities against Food Borne Pathogens.

The present study demonstrates an economical and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) using the wild mushroom Ganoderma sessiliforme. The synthesis of AgNPs was confirmed and the products characterized by UV-visible spectroscopy, dynamic light scattering spectroscopy and X-ray diffraction analysis. Furthermore, Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed to identify the viable biomolecules involved in the capping and active stabilization of AgNPs. Moreover, the average sizes and morphologies of AgNPs were analyzed by field emission scanning electron microscopy (FE-SEM). The potential impacts of AgNPs on food safety and control were evaluated by the antimicrobial activity of the synthesized AgNPs against common food-borne bacteria, namely, Escherichia coli, Bacillus subtilis, Streptococcus faecalis, Listeria innocua and Micrococcus luteus. The results of this study revealed that the synthesized AgNPs can be used to control the growth of food-borne pathogens and have potential application in the food packaging industry. Moreover, the AgNPs were evaluated for antioxidant activity (DPPH), for biocompatibility (L-929, normal fibroblast cells), and for cytotoxic effects on human breast adenosarcoma cells (MCF-7 & MDA-MB231) to highlight their potential for use in a variety of bio-applications.