Moringa Oleifera Aqueous Leaf Extract Research Articles

Effect of Moringa oleifera Leaf Aqueou Extract on Histology of Liver and Its Biochemical Parameters in Heigher Fat Diets Induced Obesity in Wistar Rats

Introduction: Obesity has emerged as a major health problem and risk factor for various disorders worldwide. Obesity has been defined as abnormal or excessive fat accumulation triggered by disproportion in energy intake than expenditure. Obesity is a non-communicable medical disorder that has largely been accepted as a reflection of healthy living and affluence among Nigerians. It is usually defined as body mass index (BMI) ≥ 30 kg/m2.
 Aim: The aim of this study was to determine the histopathological and biochemical effect of Moringa olerifera aqueous leaf extract on obesity induced albino rat.
 Methodology: A total number of 25 healthy albino rats were used. The group 1 served as negative control group consisting of 5 rats including, they were given water ad libitum and were fed with standard rat chow for 12 weeks. The group 2 consist of 5 rats, received water ad libitum and were administered orally with high-fat diet in the dose of 1ml daily of mixture of melted pig fat and hydrogenated vegetable oil in 3:2 ratio respectively for 12weeks and also fed with a standard rat chow and served as obese positive control group. The group 3 also consist of 5 rats, served as standard control group in which they were fed orally with high fat diet in a dose of 1ml of mixture of melted pig fat and hydrogenated vegetable oil in 3:2 ratio for 6 weeks and were orally administered standard drug orlistat 12mg/kg body weight daily for another 6 weeks. While group 4 and group 5 have 5 rats each received water and were administered orally with melted pig fat and hydrogenated vegetable oil 1ml daily for 6 weeks and standard rat chow after 6 weeks of feeding, moringa oleifera aqueous leaf extract in a dose of 200mg/kgbwt and 400mg/kgbwt respectively, daily for another 6 weeks the dose of extract chosen were based on the result of acute toxicity (LD50) study conducted.
 Results: The LD50 of M. oleifera is more than 5000mg/kgbwt as all doses below this incurred zero percentage of death among all groups of rats, shows no toxicity or mortality recorded after 24 hours all the animals in both Phase I and Phase II. The biochemical Parameters of Liver shows that there was no statistical significant difference in results of the parameters of all the groups when compared among the groups. Liver section shows hepatic fatty deposition when compared with control group. While A Group1 and B Group 4 Treated 200mg/kg shows no hepatic fatty deposition in hepatocytes and central vein. So also in A Group 1 and B Group 5 Treated 400mg/kg shows normal hepatocytes and central vein.
 Conclusion: This study shows that Moringa oleifera leaf extract possesses anti-hepatic fatty deposition properties like that of selected standard drugs. At higher acceptable dose of Moringa oleifera demonstrated better anti-obesity properties with the selected standard drugs. Hence, leaf extract of Moringa. oleifera is beneficial in the management of dietary obesity.

Exploring the potential of eco-friendly silver nanoparticles to inhibit azole-resistant clinical isolates of Candida spp.

The antimicrobial activity and biological efficiency of silver nanoparticles (AgNps) have been widely described and can be modeled through stabilizing and reducing agents, especially if they exhibit biocidal properties, which can enhance bioactivity against pathogens. The selective action of AgNps remains a major concern. In this regard, the use of plant extracts for the green synthesis of nanoparticles offers advantages because it improves the toxicity of Nps for microorganisms and is harmless to normal cells. However, biological evaluations of the activity of AgNps synthesized using different reducing agents are determined independently, and comparisons are frequently overlooked. Thus, we investigated and compared the antifungal and cytotoxic effects of two ecological AgNps synthesized from Moringa oleifera aqueous leaf extract (AgNp-M) and glucose (AgNp-G) against azole-resistant clinical isolates of Candida spp. and nontumor mammalian cells. Synthesized AgNps exhibited an antifungal effect on planktonic cells of drug-resistant C. albicans and C. tropicalis (MIC 0.21–52.6 µg/mL). The toxicity was influenced by size. However, the use of M. oleifera extracts allows us to obtain AgNps that are highly selective and nongenotoxic to Vero cells due to modifications of the shape and surface. Therefore, these results suggest that AgNp-M has antimicrobial potential and deserves further investigation for biomedical applications.

Nutritional and physiological responses of broiler chicken to the dietary supplementation of Moringa oleifera aqueous leaf extract and ascorbic acid in tropics.

This study was intended to determine the comparative effects of Moringa oleifera aqueous leaf extract (MOALE) and ascorbic acid supplementation in the drinking water on growth performance, haemato-biochemical profile, antioxidant status and immune response of broiler chickens under tropical climate. All 135-day-old broiler chicks were divided into 3 different treatment groups. T0 served as control, T1 were fed basal ration with MOALE (90ml/L drinking water), and T2 offered basal ration with ascorbic acid (15mg/L drinking water) for 35-day experiment. All the standard managemental practices were followed during the experimental period. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of MOALE was calculated with reference to different ascorbic acid concentration as standard. The antioxidant activity percentage of MOALE (90µl) was 93.89%, and ascorbic acid (15µg) was 98.76%, respectively, which was nearer to cent percent. Therefore, 90ml MOALE and 15mg ascorbic acid per litre drinking water, respectively, were used for supplementation in broiler chicken. The growth performance and feed efficiency were better in MOALE group followed by ascorbic acid supplemented birds without affecting the metabolism of nutrients. However, 10.71% better FCR was noted in MOALE supplemented group followed by ascorbic acid group (6.28%) in comparison with control. Most of the haemato-biochemical profiles were unaffected by the treatment except creatinine, while antioxidant profile was improved in the treatment group. Immunity status of broiler chicken against NDV was enhanced in both treatment groups; however, maximum profit is obtained in MOALE group followed by ascorbic acid supplemented birds.

Reduce the Risk of Oxidation and Pathogenic Bacteria Activity by Moringa oleifera Different Leaf Extract Grown in Sudan

High Performance Liquid Chromatography (HPLC) used in this study to identified Polyphenol constituents of Moringa oleifera leaf extract by different methods (aqueous, ethanol, ethyl acetate and chloroform), it contain gallic acid, Chlorogenic acid, Catechin, Coffeic acid, Rutin, Pyro catechol, Coumaric acid, Vanillin, Ferulic acid1, Naringenin, Propyl Gallate, 4`,7-Dihydroxyisoflavone, and Cinnamic Acid at conc. (µg/15 mg) in all extracts. Ellagic acid gave the highest concentration when extracted by ethyl acetate Caffeine gave the lowest concentration. in all different extract, The effect of moringa (aqueous, ethanol, ethyl acetate and chloroform) leaf extracts against four different pathogenic bacteria Salmonella typhimurium, Pseudomonas aeruginosa, Escherichia coli, and Bacillus cereus, were examined using Mueller Hinton Agar and measuring inhibition zone (diameter mm), were found that, there were a significant different of all moringa leaf extracts against bacteria. The study was conducted to determine the polyphenol constituents of Moringa oleifera aqueous, ethanol, ethyl acetate and chloroform leaf extract. The effect of Moringa oleifera (aqueous, ethanol, ethyl acetate and chloroform) leaf extracts against four different pathogenic bacteria.

Effects of <i>Moringa oleifera</i> Aqueous Leaf Extract on Some Serum-biochemical Constituents on Wister Rats

Medicinal plants have been used in healthcare since time immemorial. Studies have been carried out globally to verify their efficacy and some of the findings have led to the production of plant-based medicines. The global market value of medicinal plant products exceeds $100 billion per annum. This study was conducted to know the effect of Moringa oleifera leaf on Serum biochemistry of experimental exposed Wister rats. Moringa oleifera leaf was extracted with distilled water and concentrated to semisolid form with rotary evaporator at 42°C. Twenty apparently healthy rats weighing 128-233g were exposed to different concentration of Moringa oleifera aqueous leaf extract. Control group were exposed to only distill water. The animals were divided into 3 groups of five rats each, with Group-I exposed to 500 mg/kg crude extract. Group-II exposed to 1000 mg/kg crude extract and Group-III exposed to 2000 mg/kg crude extract. There significant difference (p< 0.05) in analyzed serum biochemical parameters (Creatinine, Urea, Aspartate amino transferase and Alanine amino transferase) between the experimental and control group. From this finding, it can be concluded that Moringa oleifera leaf extract up to 2000mg/kg is safe and cause slight changes in serum biochemistry. Toxicity studies of this plant extracts as well as phytochemical studies to reaffirm it effect in several body tissues and organs and identify the bioactive compounds are highly recommended.

PACKAGING OF HEPATITIS B SURFACE ANTIGEN VACCINE AND MORINGA OLEIFERA EXTRACT INTO CHITOSAN NANOPARTICLES

Objective: Oftentimes, the recombinant antigen for the use of vaccines is less immunogenic than live attenuated or inactive vaccines. Hence, a potent adjuvant is needed to enhance the immune response. Moreover, the role of vector design is also important to facilitate the improvement of the immune response. The aim of this research was to develop hepatitis B surface antigen (HBsAg)-loaded nanoparticles and Moringa oleifera aqueous leaf extracts as an adjuvant using chitosan polymer. Methods: Chitosan nanoparticles were prepared by the ionic gelation method using sodium tripolyphosphate as the cross-linking agent. A system was composed of chitosan core in which HBsAg and M. oleifera extracts were incorporated. The concentration of HBsAg used in this combination was 10 μg/ml, and the concentrations of extracts were 10, 50, and 100 μg/ml, respectively. In this study, three types of nanoparticles were produced: HBsAg-loaded nanoparticles, M. oleifera-loaded nanoparticles, and combination of HBsAg–M. oleifera-loaded nanoparticles. The nanoparticles formed were characterized by the particle size, HBsAg entrapment efficiency using sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the entrapment efficiency of extracts using the total flavonoid method. Results: The results showed that the particle size was between 111 and 245 nm. The entrapment efficiency of HBsAg in the separate formula was 79%, while that in the combined formula was approximately 96–98%. Furthermore, the entrapment efficiency of the extracts in the separate formula was around 64–91%, while that in the combined formula was 55–82.5%. Particularly, HBsAg–M. oleifera-loaded chitosan nanoparticles with the extract concentrations of 50 μg/ml showed the highest entrapment efficiencies of HBsAg and M. oleifera extracts of approximately 98 and 82.5%, respectively. Conclusion: Collectively, the system has been successfully developed, so it is then plausible to determine the function of the devices to enhance the immune response in the future.

PACKAGING OF HEPATITIS B SURFACE ANTIGEN VACCINE AND MORINGA OLEIFERA EXTRACT INTO CHITOSAN NANOPARTICLES

Objective: Oftentimes, the recombinant antigen for the use of vaccines is less immunogenic than live attenuated or inactive vaccines. Hence, a potent adjuvant is needed to enhance the immune response. Moreover, the role of vector design is also important to facilitate the improvement of the immune response. The aim of this research was to develop hepatitis B surface antigen (HBsAg)-loaded nanoparticles and Moringa oleifera aqueous leaf extracts as an adjuvant using chitosan polymer. Methods: Chitosan nanoparticles were prepared by the ionic gelation method using sodium tripolyphosphate as the cross-linking agent. A system was composed of chitosan core in which HBsAg and M. oleifera extracts were incorporated. The concentration of HBsAg used in this combination was 10 μg/ml, and the concentrations of extracts were 10, 50, and 100 μg/ml, respectively. In this study, three types of nanoparticles were produced: HBsAg-loaded nanoparticles, M. oleifera-loaded nanoparticles, and combination of HBsAg–M. oleifera-loaded nanoparticles. The nanoparticles formed were characterized by the particle size, HBsAg entrapment efficiency using sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the entrapment efficiency of extracts using the total flavonoid method. Results: The results showed that the particle size was between 111 and 245 nm. The entrapment efficiency of HBsAg in the separate formula was 79%, while that in the combined formula was approximately 96–98%. Furthermore, the entrapment efficiency of the extracts in the separate formula was around 64–91%, while that in the combined formula was 55–82.5%. Particularly, HBsAg–M. oleifera-loaded chitosan nanoparticles with the extract concentrations of 50 μg/ml showed the highest entrapment efficiencies of HBsAg and M. oleifera extracts of approximately 98 and 82.5%, respectively. Conclusion: Collectively, the system has been successfully developed, so it is then plausible to determine the function of the devices to enhance the immune response in the future.

Antihelminthic Activity in Aqueous Leaf Extract of Moringa oleifera Lam.

The objective of the study is to evaluate the antihelminthic property of Moringa oleifera aqueous leaf extract by studying the paralysis and mortality time of Tubiflex tubiflex (blood worm). The aqueous extract at 200mg/ml exhibited paralysis time of 1 minute and death time of 2 minutes which was on par with synthetic drug Albendazole at 20mg/ml. Crude condensed tannin paralysed T.tubiflex at 60 seconds with mortality time of 9 minutes at 1mg/ml. T.tubiflex was selected for the study since they share a similar anatomical and physiological aspects with the intestinal round worm. The study indicated that T.tubiflex was inhibited effectively by condensed tannin in Moringa oleifera aqueous leaf extract and perhaps might be more effective on the round worms as well, since it is consumed as vegetable for its nutritive and medicinal values in tropical and subtropical regions.

Effect of Moringa oleifera aqueous leaf extract on hepato-renal changes of albino rats induced with Salmonella typhimurium

Background: This study was designed to evaluate the effect of Moringa oleifera against Salmonella typhimurium infected changes in liver and kidney function parameters in albino rats.Methods: Twenty acclimatized albino rats were divided into four groups of five rats each (groups A-D). Groups B, C and D were exposed to 0.1ml (3.0x104 CFU) of Salmonella typhimurium. Six hours post- infection, Groups B and C were treated with 50 and 100mg/kg of Moringa oleifera aqueous leaf extract respectively and continued for 21 days. Group D was not treated while group A was neither infected nor treated. Serum alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), bilirubin, albumin, total protein, urea and creatinine were estimated using standard methods. All data were collected and analyzed in October 2015.Results: S. typhimurium infection significantly (p&lt;0.05) increased the levels of ALP, ALT, AST, bilirubin, urea and creatinine when compared with control levels (Group A). Total protein and albumin concentrations were significantly (p&lt;0.05) reduced following the infection. However, Moringa oleifera supplementation was associated with significant (p&lt;0.05) decrease in the levels of ALP, ALT, AST, bilirubin, urea and creatinine. It is also associated with decrease in concentrations of total protein and albumin.Conclusions: This study showed S. typhimurium infection induced changes in liver and kidney function parameters and also revealed possible amelioratory effects to these changes after Moringa oleifera supplementation.

Moringa oleifera aqueous leaf extract inhibits reducing monosaccharide-induced protein glycation and oxidation of bovine serum albumin.

Advanced glycation end products (AGEs) play an important factor for pathophysiology of diabetes and its complications. Moringa oleifera is one of the medicinal plants that have anti-hyperglycemic activity. However, anti-glycation property of Moringa oleifera leaf extract on the different types of reducing monosaccharides-induced protein glycation has not been investigated. Therefore, the aim of this study was to examine the protective effect of Moringa oleifera aqueous leaf extract (MOE) on reducing sugars-induced protein glycation and protein oxidation. Total phenolic content of MOE was measured using the Folin–Ciocalteu method. Bovine serum albumin was incubated with 0.5 M of reducing sugars (glucose or fructose) with or without MOE (0.5–2.0 mg/mL) for 1, 2, 3 and 4 weeks. The results found that total phenolic content was 38.56 ± 1.50 mg gallic acid equivalents/g dry extract. The formation of fluorescent and non-fluorescent AGEs [Nε-(carboxymethyl) lysine (CML)] and the level of fructosamine were determined to indicate protein glycation, whereas the level of protein carbonyl content and thiol group were examined for protein oxidation. MOE (0.5–2.0 mg/mL) significantly inhibited the formation of fluorescent, Nε-CML and markedly decreased fructosamine level (P < 0.05). Moreover, MOE significantly prevented protein oxidation manifested by reducing protein carbonyl and the depletion of protein thiol in a dose-dependent manner (P < 0.05). Thus, the findings indicated that polyphenols containing in MOE have high potential for decreasing protein glycation and protein oxidation that may delay or prevent AGE-related diabetic complications.

Moringa oleifera Lam. leaf extract as bioregulator for improving growth of maize under mercuric chloride stress

ABSTRACTThe contamination of agriculture soils with heavy metals is increasing due to both natural and anthropogenic activities. This has resulted in lowering the yield and quality of agriculture crops. The aim of the present investigation was to determine the effect of Moringa oleifera (M. oleifera) aqueous leaf extract (MALE) on growth and Hg phytoextraction potential of maize cv. Azam under mercuric chloride (HgCl2)-induced stress. Soil was contaminated with different concentrations of HgCl2 (1 and 0.5 mg/kg). The MALE was applied at 5% and 2.5% as seed soaking prior to sowing of seeds in the pots. The M. oleifera leaf was a good source of macronutrients (Na, K, Ca and Mg) and micronutrients (Fe, Zn, Mn, Co and Ni) as well as natural phenolics. The induced HgCl2 stress significantly reduced seed germination (%), shoot dry weight, root dry weight, chlorophyll content and carotenoids content. The HgCl2-induced stress was associated with accumulation of phenolics and Hg in roots. The accumulation of Hg in roots was significantly correlated with phenolics content (r = .8007, p = .000). The exogenous application of MALE significantly ameliorated adverse effects of HgCl2 stress on maize plants. The accumulation of Hg in roots was significantly increased by MALE. It is inferred from findings of the present investigation that MALE served as bioregulator and can be applied to reduce adverse effects of HgCl2 on maize. Moreover, MALE can enhance the Hg phytoremediation potential of maize.

Moringa oleifera Gold Nanoparticles Modulate Oncogenes, Tumor Suppressor Genes, and Caspase-9 Splice Variants in A549 Cells.

Gold nanoparticles (AuNP's) facilitate cancer cell recognition and can be manufactured by green synthesis using nutrient rich medicinal plants such as Moringa oleifera (MO). Targeting dysregulated oncogenes and tumor suppressor genes is crucial for cancer therapeutics. We investigated the antiproliferative effects of AuNP synthesized from MO aqueous leaf extracts (MLAuNP ) in A549 lung and SNO oesophageal cancer cells. A one-pot green synthesis technique was used to synthesise MLAuNP . A549, SNO cancer cells and normal peripheral blood mononuclear cells (PBMCs) were exposed to MLAuNP and CAuNP to evaluate cytotoxicity (MTT assay); apoptosis was measured by phosphatidylserine (PS) externalization, mitochondrial depolarization (ΔΨm) (flow cytometry), caspase-3/7, -9 activity, and ATP levels (luminometry). The mRNA expression of c-myc, p53, Skp2, Fbw7α, and caspase-9 splice variants was determined using qPCR, while relative protein expression of c-myc, p53, SRp30a, Bax, Bcl-2, Smac/DIABLO, Hsp70, and PARP-1 were determined by Western blotting. MLAuNP and CAuNP were not cytotoxic to PBMCs, whilst its pro-apoptotic properties were confirmed in A549 and SNO cells. MLAuNP significantly increased caspase activity in SNO cells while MLAuNP significantly increased PS externalization, ΔΨm, caspase-9, caspase-3/7 activities, and decreased ATP levels in A549 cells. Also, p53 mRNA and protein levels, SRp30a (P = 0.428), Bax, Smac/DIABLO and PARP-1 24 kDa fragment levels were significantly increased. Conversely, MLAuNP significantly decreased Bcl-2, Hsp70, Skp2, Fbw7α, c-myc mRNA, and protein levels and activated alternate splicing with caspase-9a splice variant being significantly increased. MLAuNP possesses antiproliferative properties and induced apoptosis in A549 cells by activating alternate splicing of caspase-9. J. Cell. Biochem. 117: 2302-2314, 2016. © 2016 Wiley Periodicals, Inc.

Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model

Background: Cadmium (Cd), an environmental metallic toxicant with varying degrees of toxicity, exists in different oxidational or transitional states and causes various blood disorders. Cadmium (Cd) is known to affect various organs on both acute and chronic exposures. Studies have revealed that the most important tissue in body in which metabolic alterations are mainly reproduced is the blood. Moringa oleifera, originally from India, has been scientifically assessed for various medicinal applications. Aims and Objective: To examine the effect of M. oleifera aqueous extract taken from the leaf on total leukocyte count including its differentials (neutrophils and lymphocytes) in cadmium-treated rats. Materials and Methods: Twenty-four adult Wistar Albino rats, weighing between 180 and 200 g were broadly divided into four groups, six animals in each group, with group I being the control. Group IV was pretreated with the extract and then cadmium chloride orally for 1 day. Data were presented as mean ± SD and p ≤ 0.05 considered significant. Result: The results portrayed that pretreatment with Moringa leaf extract, 100 mg/kg/bw, earlier to the cadmium administration exhibited a significant increase (p ≤ 0.001) in the total leukocyte count including its differentials in comparison with the cadmium-exposed group, whereas the total WBC count was significantly decreased in group III in comparison with groups I and II. This study depicts a marked increase in the total leukocyte (T-WBC) count and its differentials in the animals pretreated with Moringa oleifera leaf extract, before the infusion of cadmium could be an indication that M. oleifera offers protection against damage to hematological parameters induced by cadmium. Conclusion: Therefore, our study suggests that aqueous leaf extract of M. oleifera enhances the immune mechanisms to fight back foreign substances when exposed to cadmium toxicity.

Effects of Aqueous Extract of Moringa oleifera leaves on Epidermal Wound Healing in Domestic Rabbit

ABSTRACT A study was undertaken to assess the healing properties of Moringa oleifera leaf extract on epidermal wounds in rabbits. Twelve adult rabbits were divided into two equal groups of six. A pair of wound measuring 2cm x 2cm each was created on each rabbit. The wound on the rabbits in the experimental group were treated with Moringa oleifera aqueous leaf extracts while those in the control group were cleaned with distilled water daily. Wound contraction was measured daily with days 5, 9 and 12 representing the inflammatory, proliferative and maturation phases of wound healing respectively. Blood samples were collected on days 0, 5, 9 and 12 for analysis. Moringa oleifera leaf extract-treated animals had faster rates of wound contraction and healing with shorter days of scab fall off within 7 days; increases in packed cell volume, mean corpuscular volume, lymphocyte and neutrophil counts (P

English

The study evaluated the effect of Moringa oleifera aqueous leaf extracts on follicle stimulating hormone and serum cholesterol in Wistar rats. Thirty six (36) mature Wistar rats (20 male and 16 female rats) were used. The male rats were grouped into four groups with five animals each, while the female animals were grouped into four made up of four animals per group, on the basis of their body weights. Graded doses (1, 5 and 10 g) aqueous extract were prepared from the Mo leaves as the test samples. In the first phase: The test extract was administered orally after acclimatization to individual groups: A-male and E-female rats, 1%; groups B-male and F-female rats, 5%; and groups C-male and F-female rats, 10%. These test groups also had rat chow and water ad libitum. The second phase of the experiment involved mating the male and female animals that had the same dose of M. oleifera extract. The results show that the mean body weights of the male rats increased significantly after treatment (p&lt;0.05). The study also reveals that the administration of M. oleifera extract at different doses for the male and female rats differed significantly (p&lt;0.05) with that of the control in raising the level of follicle stimulating hormone (FSH). A-1% increased by 38.52% while B-5% decreased (-21.20%); E-1% decreased (-12.96%) and F-5% (-25.64%). After mating, the % increase in FSH concentration was observed to be significantly (p&lt;0.05) difference. Administration of Mo extract at different levels for the male and female rats differed significantly (p&lt;0.05) in this study as compared with the control in lowering the total serum cholesterol of the rats. A-1% increased total cholesterol by 1.10%, while B-5% revealed a decrease of (-10.99%). &nbsp; Key words: Moringa oleifera, follicle stimulating hormone, serum cholesterol, Wistar rats.

Moringa Oleifera aqueous leaf extract down-regulates nuclear factor-kappaB and increases cytotoxic effect of chemotherapy in pancreatic cancer cells

BackgroundFewer than 6% patients with adenocarcinoma of the pancreas live up to five years after diagnosis. Chemotherapy is currently the standard treatment, however, these tumors often develop drug resistance over time. Agents for increasing the cytotoxic effects of chemotherapy or reducing the cancer cells’ chemo-resistance to the drugs are required to improve treatment outcome. Nuclear factor kappa B (NF-kB), a pro-inflammatory transcription factor, reportedly plays a significant role in the resistance of pancreatic cancer cells to apoptosis-based chemotherapy. This study investigated the effect of aqueous Moringa Oleifera leaf extract on cultured human pancreatic cancer cells - Panc-1, p34, and COLO 357, and whether it can potentiates the effect of cisplatin chemotherapy on these cells.MethodsThe effect of Moringa Oleifera leaf extract alone and in combination with cisplatin on the survival of cultured human pancreatic cancer cells was evaluated by XTT-based colorimetric assay. The distribution of Panc-1 cells in the cell cycle following treatment with Moringa leaf extract was evaluated by flow cytometry, and evaluations of protein levels were via immunoblotting. Data of cell survival following combined treatments were analyzed with Calcusyn software.ResultsMoringa Oleifera leaf extract inhibited the growth of all pancreatic cell lines tested. This effect was significant in all cells following exposure to ≥0.75 mg/ml of the extract. Exposure of Panc-1 cells to Moringa leaf extract induced an elevation in the sub-G1 cell population of the cell-cycle, and reduced the expression of p65, p-IkBα and IkBα proteins in crude cell extracts. Lastly, Moringa Oleifera leaf extract synergistically enhanced the cytotoxic effect of cisplatin on Panc-1 cells.ConclusionMoringa Oleifera leaf extract inhibits the growth of pancreatic cancer cells, the cells NF-κB signaling pathway, and increases the efficacy of chemotherapy in human pancreatic cancer cells.

Prevention of Liver Injury by Moringa oleifera aqueous leaf extract in rats treated with isoniazid and rifampicin

Prevention of Liver Injury by Moringa oleifera aqueous leaf extract in rats treated with isoniazid and rifampicin

Toxicity potentials of the nutraceutical Moringa oleifera at supra-supplementation levels

Moringa oleifera Lam. (order –Moringales, family –Moringaceae and genus –Moringa) is a well known nutraceutical used in the treatment of hypercholesterolemia and hyperglycemia, and also, as a nutritional supplementation. Its popularity use raises the question of possible toxicity at supra-supplementation levels. The objective of the study was to ascertain possible acute toxicity with supra-supplementation using Sprague-Dawley (S-D) rats. In experiment 1, human peripheral blood mononuclear cells were given graded doses of Moringa oleifera aqueous leaf extract to induce cytotoxicity. In experiment 2, two groups of rats received low and high dose (LD and HD, respectively) levels (1000 and 3000mg/kgb.wt, respectively) per o.s. alongside negative and positive control rats (0.9% saline and 10mg/mL N-ethyl-N-nitrosourea – administered i.m., respectively). Each group consisted of five rats. Rats were killed after 48h and the femur bone marrow aspirate examined for polychromatic micronucleated erythrocytes (PCEMN)/normochromatic micronucleated erythrocytes (NCEMN) ratios after Giemsa/Leishman staining. In experiment 3, control, LD and HD groups were established. The LD and HD extracts were administered per o.s. to the respective groups and observed for 14 days. Each group consisted of five rats. Blood was sampled after 48h and 14 days and examined biochemically and haematologically for acute toxicity. Experiment 1 showed that Moringa oleifera was cytotoxic at 20mg/mL. In experiment 2, PCEMN/NCEMN ratios were: negative control=2.087; LD=1.849; HD=1.397; positive control=1.257. Statistically, LD and HD ratios were significant (p=0.020). Experiment 3 showed that hepatonephro-toxicity was nil with no abnormal haematology results. Genotoxicity results have hitherto not been shown. Moringa oleifera is genotoxic at supra-supplementation levels of 3000mg/kgb.wt. However, intake is safe at levels≤1000mg/kgb.wt.

Redox-Based Mechanisms in Diabetes

Antioxidants & Redox SignalingVol. 7, No. 11-12 Forum EditorialRedox-Based Mechanisms in DiabetesMartin J. StevensMartin J. StevensSearch for more papers by this authorPublished Online:15 Dec 2005https://doi.org/10.1089/ars.2005.7.1483AboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail FiguresReferencesRelatedDetailsCited byEffects of Moringa oleifera aqueous leaf extract in alloxan induced diabetic miceInterventional Medicine and Applied Science, Vol. 8, No. 3Protective effect of polysaccharides from Opuntia dillenii Haw. fruits on streptozotocin-induced diabetic ratsCarbohydrate Polymers, Vol. 124Redox regulation of antioxidant enzymes: post-translational modulation of catalase and glutathione peroxidase activity by resveratrol in diabetic rat liver17 April 2014 | Molecular and Cellular Biochemistry, Vol. 393, No. 1-2δ-aminolevulinate dehydratase activity in lung cancer patients and its relationship with oxidative stressBiomedicine & Pharmacotherapy, Vol. 68, No. 5Iridoid glucoside attenuates hyperglycemia-mediated oxidative stress and thyroid hormone function in streptozotocin–induced diabetic ratsBiomedicine & Preventive Nutrition, Vol. 4, No. 1Amelioration of alloxan-induced diabetic keratopathy by beta-caroteneExperimental and Toxicologic Pathology, Vol. 66, No. 1Myricetin modulates streptozotocin–cadmium induced oxidative stress in long term experimental diabetic nephrotoxic ratsJournal of Functional Foods, Vol. 5, No. 3In vitro upregulation of erythrocytes glucose uptake by Rhaphnus sativa extract in diabetic patientsBiochimie, Vol. 94, No. 5Phenol-Based Antioxidants and the In Vitro Methods Used for Their Assessment29 February 2012 | Comprehensive Reviews in Food Science and Food Safety, Vol. 11, No. 2Effects of resveratrol on biomarkers of oxidative stress and on the activity of delta aminolevulinic acid dehydratase in liver and kidney of streptozotocin-induced diabetic ratsBiochimie, Vol. 94, No. 2Hypoglycaemic, antioxidative and nephroprotective effects of taurine in alloxan diabetic rabbitsBiochimie, Vol. 91, No. 2Lipoic acid ameliorates oxidative stress and renal injury in alloxan diabetic rabbitsBiochimie, Vol. 90, No. 3 Volume 7Issue 11-12Nov 2005 InformationCopyright 2005, Mary Ann Liebert, Inc.To cite this article:Martin J. Stevens.Redox-Based Mechanisms in Diabetes.Antioxidants & Redox Signaling.Nov 2005.1483-1485.http://doi.org/10.1089/ars.2005.7.1483Published in Volume: 7 Issue 11-12: December 15, 2005PDF download

ROLE OF MORINGA OLEIFERA LEAF EXTRACT IN THE REGULATION OF THYROID HORMONE STATUS IN ADULT MALE AND FEMALE RATS

The role of Moringa oleifera aqueous leaf extract in the regulation of thyroid hormone status, was studied in adult Swiss rats. Other than the thyroid hormone concentrations, hepatic lipid peroxidation (LPO) and the activities of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) were evaluated. In the first experiment, effects of the leaf extract (175 mg kg−1body wt. day−1for 10 days) were studied both in male and female animals. Following the administration of the extract, serum triiodothyronine (T3) concentration and hepatic LPO decreased with a concomitant increase in the serum thyroxine (T4) concentration, in female rats, while in males no significant changes were observed, suggesting that Moringa oleifera leaf extract is more effective in females than in the males. To evaluate the impact of a higher dose, in the second experiment, the study was repeated in female rats, with 350 mg kg−1body wt. day−1for the same duration. Almost similar reduction in the serum T3concentration (approx. 30%) and an increase in the T4concentration were observed suggesting the inhibiting nature of Moringa oleifera leaf extract in the peripheral conversion of T4to T3, the principal source of the generation of latter hormone. As the antiperoxidative effects were exhibited only by the lower dose and percent decrease in T3concentration was nearly the same by both the doses, it is suggested that the lower concentration of this plant extract may be used for the regulation of hyperthyriodism. 2000 Academic Press@p$hr