Months Of Preservation Research Articles

Profile of Sui Wu’u, a Fermented Pork Product Stored in Plastic Containers and Bamboo Tubes

Abstract Sui Wu’u is an Indonesian fermented pork dish made from a mixture of pork belly, corn flour, and salt. This study evaluated the physicochemical, microbiological, and sensory properties of Sui Wu’u after three months of preservation in bamboo tubes (Swbt) and polypropylene plastic containers (Swpp). Different storage containers had no effect on the pH value, water activity, fat, protein, fatty acid composition, selenium, or zinc levels of Sui Wu’u but significantly affected its moisture, potassium, and phosphorus levels. Sui Wu’u were high in MUFA, followed by SFA and PUFA, with oleic, palmitic, stearic, and linoleic acids being the most prominent fatty acids. Sui Wu’u also contained 18 amino acids, with the highest levels of glycine, proline, and arginine in Swbt. Sui Wu’u was slightly moist and soft in texture when preserved in both containers, but its color was lighter in Swpp, and its aroma was pungent in Swbt. This did not affect the acceptability of the panelists. This investigation showed that Sui Wu’u may be stored for 3 months in both Swbt and Swpp containers with good preservation. However, Swpp offers an alternative to Sui Wu’u storage due to its efficiency, mobility, and accessibility.

Biochemical potentials and stability of Oxalis corniculata L. leaf extracts

Biochemical activity and its stability of Oxalis corniculata L. leaf extracts in methanol, cyclohexane and water were investigated in this study. Preliminary phytochemical screening of the total phenolic and flavonoid contents for the methanolic extract were estimated as 18.63 ± 0.55 mg gallic acid equivalent and 6.46 ± 0.85 mg quercetin equivalents per gram of fresh leaf respectively. Maximum flavonoid content was observed in cyclohexane extract and the phenolics of aqueous extract were relatively stable as compared to the other extracts. The estimated total antioxidant capacity and the total reducing power of methanolic extract were the highest among the extracts, and were 12.33 ± 0.54 mg and 3.79 ± 0.37 mg ascorbic acid equivalent per gram of fresh leaf respectively. The IC50 values of DPPH free radical scavenging activity and hydrogen peroxide scavenging activity of the methanolic extract of fresh leaf were 10.14 ± 0.04 mg and 11.1 ± 0.01 mg, respectively. Such activities were absent or negligible in other extracts. Thin layer chromatographic studies detected the presence of at least two compounds with potent antioxidant activity. However, the phenolic and flavonoid contents, and all biochemical activities of the extracts were reduced after six months of preservation at -20oC. These data indicated that freshly prepared methanolic extract of O. corniculata could be the most potent, and such fresh preparation should be used to attain the desirable biochemical activities. Dhaka Univ. J. Biol. Sci. 34(1): 75-83, 2025 (January)

Đánh giá hiệu quả bảo quản đông khô chủng vi khuẩn và xạ khuẩn biển bằng kỹ thuật tiền xử lý lạnh sâu và sử dụng chất bảo vệ

This study aimed to evaluate the effect of deep freezing pretreatment techniques and the protectants on the efficiency of freeze-drying preservation of Bacillus amyloliquefaciens ĐL01.B09 (Genbank OQ415515) and Streptomyces sp. TA3.A03 (Genbank OQ415510) isolated from marine sediments in Truong Sa archipelago (Spratly Islands), Khanh Hoa province. The results showed that the survival rate of both strains ĐL01.B09 and TA3.A03 in the sample pretreatment formula at -80°C was higher than at -20°C and higher than the control without deep freezing pretreatment, the survival rate reached 71% with actinomycetes and 73% with bacteria. When the two microorganism strains were deep freezing pretreatment combined with the use of protectants including lactose, sucrose, trehalose, and skim milk, their survival rates were 51% - 80%, which is significantly greater than the control (21% - 18%). Using a combination of protectants including trehalose 10%, skim milk 10%, sodium glutamate 1% and NaCl 0.9% and deep freezing pretreatment at -80°C provided the best protective effect, the survival rates were 91%-88% and the density of microorganisms after freeze-drying reached ≥ 1010 CFU/mL. The quality of freeze-dried microbial strains met Vietnam's standard TCVN 6168 - 2002 after 15 months of preservation at room temperature.

Production and bioprocessing of Taxol from Aspergillus niger, an endophyte of Encephalartos whitelockii, with a plausible biosynthetic stability: antiproliferative activity and cell cycle analysis

The biosynthetic potency of Taxol by fungi raises their prospective to be a platform for commercial production of Taxol, nevertheless, the attenuation of its productivity with the fungal storage, is the challenge. Thus, screening for a novel fungal isolate inhabiting ethnopharmacological plants, with a plausible metabolic stability for Taxol production could be one of the most affordable approaches. Aspergillus niger OR414905.1, an endophyte of Encephalartos whitelockii, had the highest Taxol productivity (173.9 μg/L). The chemical identity of the purified Taxol was confirmed by HPLC, FTIR, and LC–MS/MS analyses, exhibiting the same molecular mass (854.5 m/z) and molecular fragmentation pattern of the authentic Taxol. The purified Taxol exhibited a potent antiproliferative activity against HepG-2, MCF-7 and Caco-2, with IC50 values 0.011, 0.016, and 0.067 μM, respectively, in addition to a significant activity against A. flavus, as a model of human fungal pathogen. The purified Taxol displayed a significant effect against the cellular migration of HepG-2 and MCF-7 cells, by ~ 52–59% after 72 h, compared to the control, confirming its interference with the cellular matrix formation. Furthermore, the purified Taxol exhibited a significant ability to prompt apoptosis in MCF-7 cells, by about 11-fold compared to control cells, suppressing their division at G2/M phase. Taxol productivity by A. niger has been optimized by the response surface methodology with Plackett–Burman Design and Central Composite Design, resulting in a remarkable ~ 1.6-fold increase (279.8 μg/L), over the control. The biological half-life time of Taxol productivity by A. niger was ~ 6 months of preservation at 4 ℃, however, the Taxol yield by A. niger was partially restored in response to ethyl acetate extracts of E. whitelockii, ensuring the presence of plant-derived signals that triggers the cryptic Taxol encoding genes.

Effect of growth medium composition on the viability and genetic stability of Chrysanthemum × morifolium Ramat. explants under in vitro cold storage conditions

Cultivation of plants at low positive temperatures is promising for the in vitro preservation of valuable Chrysanthemum × morifolium Ramat. varieties. The study aims to examine the effect of chlorocholine chloride concentrations in a specialized medium on the viability and the physiological and morpho-anatomical parameters of chrysanthemum plants (Excel, Sheer Purple, and William Seward varieties), as well as their genetic stability after 12 months of cold storage under in vitro gene bank conditions. In vitro microshoot tips (without leaves) were placed on a 1/4 Murashige and Skoog medium supplemented with 60.0 g/L sucrose and 0.2–0.8 g/L chlorocholine chloride. The explants were stored in cooling chambers at a temperature of 4 °C. After 12 months of preservation, a reduction was observed in growth kinetics with increasing concentration of chlorocholine chloride in the medium. The viability of explants decreased from 96 to 91% at higher concentrations of chlorocholine chloride. The effective photochemical quantum yield of photosystem II decreased more than twice under cold storage conditions as compared to the control. Several histological features were identified in the leaves of in vitro explants cold-stored in the gene bank: decreased in leaf thickness and mesophyll density in leaf blades, degradation of palisade tissue, and a high degree of transpiration regulation. The SSR and ISSR analyses were used to show that the microshoots of the examined chrysanthemum varieties were identical to the original plants following in vitro cold storage. The conducted studies revealed that the presence of 0.6–0.8 g/L chlorocholine chloride in the medium reduces the growth kinetics of explants while maintaining their high viability and genetic stability.

Preservation of Goat Kidneys Using Low Concentration Formalin for Human Anatomy Learning

<p><strong>Background: </strong>Exposure to formaldehyde from a preserved cadaver can cause several health problems in students, lecturers, and laboratory technicians working in medical anatomy laboratories. These conditions include respiratory tract irritation and cancer due to acute and chronic exposure, respectively. Lowering the frequency of contact with formaldehyde from preserved organs helps to ensure the safety of students, lecturers, and laboratory technicians. Therefore, this study aims to determine the effectiveness of low concentration formalin as a preservative solution for goat kidneys. The organ was selected due to its anatomical structure similarity to humans.</p><p><strong>Method</strong>: Goat kidneys with 50-60 ml of volume were collected, washed, immersed in preservative solution, and stored for two months. The samples were then divided into four groups, which were preserved with different formalin concentrations, namely 30%, 20%, 10%, and 5%. The preservation parameters, namely organ structure and integrity, color, springiness, odor, and mold growth were qualitatively described, while the kidney volume was quantitatively measured.</p><p><strong>Results</strong>: After the samples were preserved for two months, they were observed, and all groups showed similar characteristics. The kidneys preserved with 5% or 10% formalin showed equal volume before and after two months of preservation.</p><p><strong>Conclusion</strong>: Low concentration formalin has the same effectiveness in preserving goat kidneys as the high concentration. It also has the potential to be applied in human anatomy learning.</p>

SURGICAL TREATMENT OF UNICISTIC AMELOBLASTOMA AFTER 11 YEARS RELAPSE - CASE REPORT

SURGICAL TREATMENT OF UNICISTIC AMELOBLASTOMA AFTER 11 YEARS RELAPSE - CASE REPORT

Effects of sugar addition on adipolysis and volatile profiles of dry cured sausage

Effects of sugar addition on adipolysis and volatile profiles of dry cured sausage

Cirurgia periodontal guiada – fluxo digital para correção de sorriso gengival

To promote an effective and predictable treatment planning in cases of gingival smile correction, a periodontal surgical guided from virtual planning could be used. The guide was designed using planning software, based on the patient’s facial aesthetic analysis, photos and intraoral scanning. The present case report describes a gingival smile correction, using digital planning combined with high-power diode laser for periodontal surgery. After 16 months of preservation, there was less exposure of the gingiva in the smile and a high level of patient aesthetic satisfaction. Considering the importance of the correct treatment planning, it may be concluded that use of surgical guided made from a digital planning provided a predictable, personalized and safe treatment to the patient.

Possibility of Pulsed Electric Field and Essential Oil Pre-treatment, Microwave-air Dehydration to the Quality of the Dehydrated Sesban (Sesbania sesban) Flower

Non-heat ahead-treatment in advance of the main dehydration is essential to preserve the quality and ensure food safety. Pulsed electric field (PEF) utilizes a high-voltage electric field in a very short duration to inhibit microbes and enzymes while maintaining the most sensory and nutritional characteristics. For thermal sensitive components, the dehydration process should be performed at low temperatures. Freeze dehydration, vacuum dehydration required high cost for equipment, energy consumption, low quantity in long dehydration time. Microwave-air dehydration is considered as a promising alternative technical approach. Sesban (Sesbania sesban) flower contains numerous phytochemical components promoting health-benefit. However, it’s highly perishable after harvesting. Consumers enjoy the dried sesban flower as a healthy drink. This study examined the possibility of PEF ahead-treatment in microbial inhibition and enzymatic inactivation; essential oil and Microwave-air dehydration on retention of total phenolic content (TPC), vitamin C, 2,2 diphenyl-1-picrylhydrazyl of free radical scavenging (DPPH), ferric reducing antioxidant power (FRAP) of the dehydrated sesban flower. Research also monitored the microbial stability of the dehydrated sesban flower during 12 months of preservation. Results showed that PEF at pulse strength 1000 kV/cm, pulse duration 90 µs, pulse number 45 was remarkably inactivated polyphenol oxidase and peroxidase in raw material. Rosemary essential oil soaked for sesban flower before dehydration positively preserved the ascorbic acid, phenolic content and antioxidant capacity. These PEF and essential oil ahead-treatments strongly facilitated for the main Microwave-air dehydration. Among different air temperatures from 20°C to 40°C in microwave-air dehydration, the highest Vitamin C, TPC, DPPH and FRAP of the dried flower were recorded at air temperature from 20°C to 30°C with no significant difference. Meanwhile, airspeed 1.2 m/s showed the highest Vitamin C, TPC, DPPH and FRAP of the dried flower with no significant difference with airspeed 1.4 m/s and 1.6 m/s. There was no significant difference in Vitamin C, TPC, DPPH and FRAP of the dried flower by microwave power from 1.15 to 1.45W/g. Therefore, a combination of microwave and air dehydration at air temperature 25°C, airspeed 1.2 m/s, the microwave energy density of 1.45 W/g was recommended to better preserve vitamin C, TPC, DPPH, FRAP. Microbial stability of the dehydrated flower was also observed during 12 months of storage by 3 month-interval sampling. Coliform, yeast and mold criteria in dried product were stable within acceptable limits.

SUGGESTION FOR AN OPTIMAL MODEL FOR E.coli, S. aureus AND C. albicans PRESERVATION AT A STORAGE TEMPERATURE IN THE RANGE OF 4°C TO -20°C

The low temperature in the storage processes can vary in the range of +4°C to -80°C and even lower, using liquid nitrogen. Depending on this, the time for which we expect the microbial culture to remain viable also changes. Agar slant culture, covered with oil, stab culture, saline suspension, glycerol and DMSO preservation, drying on silica gel, drying on soil, sterile water, lyophilization, cryopreservation, etc. are methods employed for the preservation of microorganisms. However, the choice of method to be used depends on the type of microorganism, the purpose of storage, and duration of preservation. The aim of the study is to compare storage techniques using semisolid agar and cultivation at 4°C and using BHI broth with glycerol and cultivation at -20°C. These are two commonly used and accessible methods for bacteria and yeast preservation. After performing the storage procedure for a total of 18 strains of S. aureus, E. coli and C. albicans, we re-cultivated them after two and six months of preservation. From the obtained results, we can conclude that the storage of bacteria at 4°C on semisolid agar for up to 8 weeks is successful, in C. albicans, one of the tested six strains did not survive. After 6 months, another strain of the yeasts did not show growth, as well as one of the sixth E. coli strains. Storing microbes at -20°C with a cryoprotectant has proven to be a more successful method. This was an expected result, and other authors commented that lower storage temperatures provide longer life for microorganisms.

Guided Periodontal Surgery: Digital Workflow for Correction of a Gingival Smile

To promote an effective and predictable treatment planning in cases of gingival smile correction, a periodontal surgical guided from virtual planning could be used. The guide was designed using planning software, based on the patient’s facial aesthetic analysis, photos and intraoral scanning. As a result, the surgical procedure by digital planning provided a more predictable, personalized and safe treatment and outcome to the patient. The present case report describes a gingival smile correction, using digital planning combined with high-power diode laser for periodontal surgery. After 3 months of preservation, there was less exposure of the gingiva in the smile and a high level of patient aesthetic satisfaction. Considering the importance of the correct treatment planning, it may be concluded that use of surgical guided made from a digital planning provided a predictable, personalized and safe treatment to the patient.

Toward electron-beam sterilization of a pre-assembled Boston keratoprosthesis

Toward electron-beam sterilization of a pre-assembled Boston keratoprosthesis

Illumina MiSeq sequencing reveals microbial community succession in salted peppers with different salinity during preservation

Illumina MiSeq sequencing reveals microbial community succession in salted peppers with different salinity during preservation

MALDI-TOF MS: optimization for future uses in entomological surveillance and identification of mosquitoes from New Caledonia

BackgroundMosquito vectors cause a significant human public health burden through the transmission of pathogens. Due to the expansion of international travel and trade, the dispersal of these mosquito vectors and the pathogens they carry is on the rise. Entomological surveillance is therefore required which relies on accurate mosquito species identification. This study aimed to optimize the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for mosquito identification.MethodsAedes aegypti of the Bora-Bora strain and 11 field-sampled mosquito species were used in this study. Analyses were performed to study the impact of the trapping duration on mosquito identification with MALDI-TOF MS. The best preservation methods to use for short, medium and long-term preservation before MALDI-TOF MS analysis were also assessed. In addition, the number of specimens per species required for MALDI-TOF MS database creation was determined. The first MALDI-TOF database of New Caledonian mosquitoes was assembled and the optimal threshold for mosquito species identification according to the sensitivity and specificity of this technique was determined.ResultsThis study showed that the identification scores decreased as the trapping duration increased. High identification scores were obtained for mosquitoes preserved on silica gel and cotton at room temperature and those frozen at − 20 °C, even after two months of preservation. In addition, the results showed that the scores increased according to the number of main spectrum patterns (MSPs) used until they reached a plateau at 5 MSPs for Ae. aegypti. Mosquitoes (n = 67) belonging to 11 species were used to create the MALDI-TOF reference database. During blind test analysis, 96% of mosquitoes tested (n = 224) were correctly identified. Finally, based on MALDI-TOF MS sensitivity and specificity, the threshold value of 1.8 was retained for a secure identification score.ConclusionsMALDI-TOF MS allows accurate species identification with high sensitivity and specificity and is a promising tool in public health for mosquito vector surveillance.

Long-term Preservation of Micro-algal Stock for Fish Hatcheries

Long-term Preservation of Micro-algal Stock for Fish Hatcheries

Chemical hydrolysis of oleuropein affected by the type of organic acid

Chemical hydrolysis of oleuropein affected by the type of organic acid

Application of xylo-oligosaccharide as natural emulsifer in dry-fermented shrimp sausage

Present study was focused on the feasibility of utilization of broken shrimp fortified with xylo-oligosaccharide as emulsifier in physico-chemical, microbiological, and sensory attributes of the dry fermented sausage. Different ratios of xylo-oligosaccharide (0.5, 0.75, 1.0, 1.25 and 1.5%) blended with broken shrimp batter were verified. Stability of the dried shrimp sausage was also observed during 6 months of storage in vaccum packaging. Our results showed that the dried fermented shrimp sausage fortified by 1.25% xylo-oligosaccharide had strong texture firmness (N) with low water activity (aw), low microbial load (cfu/g) and high sensory score. Shelf-life of this dry-fermented sausage was stable during 6 months of preservation without any deterioration.

TREATMENT BY ENUCLEATION OF NASOLABIAL CYST - CASE REPORT

TREATMENT BY ENUCLEATION OF NASOLABIAL CYST - CASE REPORT

Effect of calcium on the preservation of green olives intended for black ripe olive processing under free-sodium chloride conditions

Effect of calcium on the preservation of green olives intended for black ripe olive processing under free-sodium chloride conditions