Abstract Type 2 diabetes represents a prevalent serious health problem worldwide. Type 2 diabetes (non-insulin-dependent) is characterized by chronic hyperglycemia and disturbances of carbohydrate, fat and protein metabolism. Complications among diabetic patients include increased recurrence and severity to many infections. Low-grade inflammation also seems to be involved in the pathogenesis of diabetes. However the underlying mechanisms of the immunological impairment in diabetes are unclear. Toll-like receptors (TLRs) play a key role in pathogen recognition by the innate immunity, interestingly altered TLR expression has been recently reported in diabetic rodent models but it has never been studied in humans. Therefore using flow cytometry we tested if, under basal conditions, TLR expression was altered in peripheral blood monocytes and granulocytes from Type 2 diabetic Mexican subjects. We found a decreased frequency of monocytes and granulocyte expressing TLR3 and TLR4 in diabetic subjects, nevertheless TLR2 and TLR9 expression was not significantly different between diabetic and healthy groups. Whereas in hyperglycemic conditions lower frequencies of TLR2, TLR3 and TLR4 expression were found, TLR9 expression was significantly higher. Our findings support that the increased incidence of infections occurring in diabetes maybe related an impaired TLR expression.