The Yellow River is known as the "mother river" of Chinese civilization, which not only nurtures the Chinese nation but also has unique cultural characteristics. Folk songs, as an important carrier of inheriting traditional Chinese culture, embodies the wisdom of the Chinese people and provide strong impetus for promoting social development. The middle and upper reaches of the Yellow River connect Qinghai-Tibet Plateau, the Inner Mongolian Plateau, Hetao Plain, Loess Plateau and other regions, forming an overall combination, organically integrating nomadic civilization and agricultural civilization, then a unique culture of the Yellow River basin has been formed. Along this cultural belt, cultural integration among multiple ethnic groups in various regions has developed a unique musical and cultural pattern in the Yellow River Basin. Therefore, studying the integration characteristics and cultural connotations of folk songs in the middle and upper reaches of the Yellow River is of great significance for inheriting Yellow River culture and promoting traditional Chinese culture of folk songs.