Sugarcane viral diseases: Epidemiology, detection, and advanced breeding methods for resistance.

Negative regulation of ZmBBX24 gene under combined light and drought stress modulates drought tolerance in Arabidopsis.

The soybean gene GmSP1L-8 improves salt tolerance through the enhancement of the ROS scavenging system.

Environmental control of flowering through alternative splicing of FLC and COOLAIR.

Genomic selection study on resistance to Cryptocaryon irritans in turbot (Scophthalmus maximus)

PmPPO-mediated drought adaptation mechanism in Prunus mira Koehne: Interaction with PmRad23d and regulation of ROS homeostasis.

Genome-wide association study and transcriptome analysis identify candidate genes associated with low nitrogen-induced root plasticity in Zea mays L.

Genome-wide association study for salinity tolerance traits in grass carp (Ctenopharyngodon idella)

Systematic study of genomic loci in Escherichia coli B and K12 for genomic integration: application in plasmid-free astaxanthin production.

The GOLDEN2-LIKE (GLK) gene family, known for its role in chloroplast development, has recently been implicated in involvement of anthocyanin biosynthesis in kiwifruit (Actinidia spp.), but the underlying regulatory mechanism remains unclear. Here we report the characterization of a kiwifruit GLK homolog AcGLK2 in regulating anthocyanin accumulation. We found that expression of AcGLK2 is much higher exclusively in the red pigment-accumulated fruit tissue. Overexpression of AcGLK2 in Arabidopsis and kiwifruit significantly enhanced anthocyanin content, whereas its RNAi-mediated silencing compromised anthocyanin accumulation. RNA-Seq analysis revealed significant upregulation of many structural genes and transcription factors (TFs) associated with the flavonoid pathway in AcGLK2-overexpressing kiwifruit. ChIP-Seq analysis indicated that AcGLK2 directly binds to AcMYB5 and AcTRY. We showed AcMYB5, an R2R3-MYB TF, promotes anthocyanin accumulation by interacting with the bHLH protein AcbHLH42, while AcTRY, an R3-MYB protein, competitively inhibits the interaction between key MYBs and AcbHLH42, thereby repressing anthocyanin biosynthesis. Transgenic and molecular assays in tobacco, tomato, and kiwifruit demonstrated that AcGLK2 positively participates into regulation of anthocyanin accumulation through transcriptionally activating AcMYB5 expression and concomitantly suppressing AcTRY expression. This study reveals the dual regulatory mechanism of AcGLK2 in anthocyanin biosynthesis, broadening the understanding of GLK gene functions and providing a valuable genetic resource for molecular breeding of nutritional quality in kiwifruit and other crops.

Genome-wide identification of AcPP2C gene family and functional characterization of AcPP2C40 in response to heat stress in kiwifruit.

Integrated transcriptomic and metabolomic analysis provides insights into the growth advantage in triploid Pacific abalone (Haliotis discus hannai)

Rice cold tolerance: Physiological responses, molecular regulatory networks, and improvement strategies.

Internode morphology critically determines bamboo wood quality. Phyllostachys edulis 'Heterocycla' (GJ) is a natural dwarf mutant of P. edulis (WT), yet regulatory mechanisms remain unclear. Here, we show that impaired parenchyma cell elongation is the primary cellular basis for dwarfism in GJ. This phenotype correlates with a marked reduction in gibberellin (GA) and an increase in abscisic acid (ABA) levels. Through weighted gene co-expression network analysis, we identified PeGA20ox1 as a critical regulator of internode elongation. In GJ, PeGA20ox1 undergoes alternative splicing (AS), producing a transcript, PeGA20ox1-GJ, with a premature termination codon. Moreover, we characterized that a bifunctional transcription factor PeRAP2-1 mediates GA/ABA homeostasis, which is an activator of PeABA3 and an inhibitor of PeGA20ox1 by binding to different cis-acting elements. Intriguingly, this AS event results in the loss of function of PeGA20ox1-GJ, which disrupts the PeRAP2-1-mediated hormonal balance, ultimately inhibiting internode elongation. This study suggests that the posttranscriptional regulation induced by AS may adapt to the rapid growth of bamboo plants and provides important genetic resources for the molecular breeding of bamboo with improved culm traits.

Genome-wide selection and association analyses implicate retinal and reproductive genes in laying-period variation of domestic geese.

Application of genomic selection in Korean abalone by evaluating growth traits using PBLUP, GBLUP, and ssGBLUP models

Genome-wide association study for the detection of genetic variants associated with the antibody response upon viral hemorrhagic septicemia virus vaccination in Paralichthys olivaceus.

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Disruption of Pik3r1 promotes muscle hyperplasia and lipolysis in grass carp (Ctenopharyngodon idella).

The Mozambique tilapia (Oreochromis mossambicus) is a species of significant ecological and economic importance in Southern Africa. However, urbanization and water management challenges have led to the species being classified as threatened by the International Union for Conservation of Nature. Despite its widespread distribution and significance as a key food source, the genetic architecture of Southern African O. mossambicus remains inadequately characterized. This gap in knowledge hinders efforts to conserve and manage the species effectively. This study presents a high-quality chromosome-level genome assembly for a South African O. mossambicus assembled using both PacBio HiFi and Dovetail Omni-C sequencing. The assembled genome span 1.09 Gb genome size which is highly contiguous (Contig N50 = 35.6 Mb, scaffold N50 = 42.8 Mb), with 99.4% of sequences anchored into 22 chromosomes and a BUSCO completeness of 97.5%. We annotated 27,544 protein-coding genes. This genome provides an essential resource for preserving native biodiversity and enabling genomic selection for traits like disease resistance and growth performance within the South African aquaculture context.