Modular Complex Research Articles

Mutation in a flexible linker modulates binding affinity for modular complexes.

Tandem beta zippers are modular complexes formed between repeated linear motifs and tandemly arrayed domains of partner proteins in which β-strands form upon binding. Studies of such complexes, formed by LIM domain proteins and linear motifs in their intrinsically disordered partners, revealed spacer regions between the linear motifs that are relatively flexible but may affect the overall orientation of the binding modules. We demonstrate that mutation of a solvent exposed side chain in the spacer region of an LHX4-ISL2 complex has no significant effect on the structure of the complex, but decreases binding affinity, apparently by increasing flexibility of the linker.

Development of Technologies and Mobile Modular Complexes for Getting Liquid Products from Associated Petroleum Gas

Various technologies and equipment complexes for associated petroleum gas (APG) stripping are analyzed and modeled to develop a universal technology and mobile modular complexes for getting liquid products from APG. The selection of a schematic design of an APG stripping plant that includes a refrigerating machine operating with a multicomponent mixed refrigerant and a fractionating column is validated.

Учебно-методический модульный комплекс для подготовки специалистов в области автоматизации технологических процессов по пусконаладке промышленных автоматизированных установок

Учебно-методический модульный комплекс для подготовки специалистов в области автоматизации технологических процессов по пусконаладке промышленных автоматизированных установок

Raw peat production and processing from flooded fields and approaches to maintain dehydration

The autonomous modular complex is energy efficient, capable to ensure year-round production of raw peat and its onboard processing thus transforming some part of fuel into thermal and electrical energy provided the complex is self-powered and generates energy for local consumers. The integrated scheme of modular units and energy intensity of raw peat production and processing depends on the preferred method of raw materials extraction. In general, the functional structure of a complex may include the following units: raw peat production, separation and crushing, formation of peat (raw peat) reserves, drying, generation of electrical energy for internal needs, production of commodity products (granules, gas, coke, fertilizers, electrical energy, etc.). To comply with the energy saving principle the autonomous production and processing complex with power self-generation focuses on such method of peat mechanical dehydration as gravity filtration and sedimentation. Magnetic treatment of a peat pulp may be used to intensify the moisture disengagement under the influence of gravity forces. The analysis of water separation intensity on a pulp did not reveal a clear influence of a constant magnetic field. However, the influence of an alternating magnetic field revealed explicit (more than twice) increase in the intensity of water separation in comparison with the basic test.

Neural network, blockchain, and modular complex system: The evolution of cyber-physical systems for material flow analysis and life cycle assessment

Neural network, blockchain, and modular complex system: The evolution of cyber-physical systems for material flow analysis and life cycle assessment

Repeated and Folded DNA Sequences and Their Modular Ag106+ Cluster

Molecular silver clusters are optical chromophores, and species with distinct spectra form with DNA strands. One such hybrid chromophore is a violet cluster bound to repeated C2X sequences where X ≠ C. We varied the number of C2X repeats and the X nucleobase and consider three observations. First, different lengths of (C2A)y and (C2T)y strands with y = 3–12 identify a minimal (C2X)6 scaffold that forms a specific Ag10 adduct. This cluster has a +6 oxidation state, absorbs between 400–450 nm, and folds its DNA host. Second, different X nucleobases alter the (C2X)6 binding site. The natural nucleobases preferentially form the Ag106+ cluster and yield strong circular dichroism. These ligands coordinate via their heteroatoms, and the N3 of thymine was identified via cluster fluorescence that varies with pH. In contrast, abasic sites and imidazole substitutions suppress circular dichroism and diminish the number of silver adducts. These observations suggest that a (C2X)6 coordinates Ag106+ via multiple nucleobases. Third, beyond the minimal (C2X)6 binding site, longer strands still form Ag106+ but can also coordinate additional Ag+ adducts. The added Ag+ do not perturb the Ag0 and their spectra and thus may partition to open C2X subunits outside the core (C2X)6–Ag106+ complex. Thus, these modular complexes distinguish oxidized and reduced silvers. Collectively, these three observations suggest that the DNA and silver cluster play complementary roles: a repeated C2X sequence stabilizes the Ag106+ cluster, while the cluster folds its host. We specifically suggest that the Ag+ within Ag106+ cross-links remote C2X subunits and the Ag0 coordinate with mismatch sites in a hairpin-like secondary structure. Distinct roles for Ag+ and Ag0 within a cluster are considered in light of the X-ray spectra of related complexes.

HYBRID RSA based highly efficient, reliable and strong personal Full Mesh Networked messaging scheme

Efficient balancing of privacy and strong authentication in end-to-end (E2E) security constitutes a challenging task in the field of personal messaging. Since RSA is a ubiquitous approach, we here propose a hybrid RSA-based, highly efficient, reliable and strong personal full mesh networked messaging scheme. M-prime RSA and CRT-RSA with shared RSA makes our hybrid RSA decryption much more secure and efficient and protects our users with complete privacy. However, computational modular exponentiation complexity and partial key exposure vulnerability of RSA present two major obstacles. Low modular complexity and asymptotic very slow speed of decryption of RSA, with the ease and speed problem in encryption of RSA are also problems to be solved. Our hybrid RSA cipher resolves all of the above issues and provides protection against exploitation of multiplicative property and homomorphic property of RSA. Our full mesh networking scheme also ensures E2E encryption for all peers. So, our three-way authenticated hybrid RSA messaging scheme achieves a perfect balance of efficiency, security, authentication, reliability and privacy. Consequently, our scheme offers a smarter choice for private messaging in existing, as well as future, internet architectures.

PRACTICAL IMPLEMENTATION OF HARDWARE AND SOFTWARE COMPLEX FOR PLANAR MEASUREMENTS OF ANTENNA CHARACTERISTICS IN THE NEAR ZONE

Directional patterns measurement of antennas is carried out by the methods of far and near zone. Measurements in the far zone are straightforward; however they have a number of disadvantages. Near zone measurement methods are free from measurement shortcomings in the far zone, but it requires complex and expensive equipment for its implementation.Earlier, the authors have developed a concept of hardware and software modular design complex to determine antenna system characteristics as per measurements in the near zone. This concept assumes creation of a universal measuring complex to investigate various types of antenna systems with any type of measurement surface (plane, cylinder, sphere) in order to solve a wide range of applied problems. The purpose of this work lies in practical implementation of a variant of this measuring complex for measurement along the plane and determination of its metrological (hardware and software) capabilities.A working experimental sample of hardware and software complex for measuring the characteristics of antenna systems that realizes a radio-holographic method for measuring along a plane has been developed, created and practically tested. A preliminary estimation of errors in amplitude and phase measurements in the dynamic range of 45 dB and a comparison of the characteristics of several types of antennas measured in far and near zones have been made. Algorithms have been developed, a software for processing, storing and graphical display of measurement results has been created.

INNOVATIVE TECHNOLOGIES OF LOGISTICAL SUPPORT OF TROOPS

Purpose. Comprehensive and uninterrupted logistical support of troops is considered one of the main factors of successful conduct of hostilities by any state. The logistics support system, created in peacetime, is intended for use in the course of a military company. It is the link between the front and the economy of the country, and at the same time, it is one of the important factors affecting the combat operation. According to experts, changes in the geopolitical and military-strategic situation in Europe resulted also changes in the armed forces of states, including their logistical support, which is undergoing changes and is developing under the direct influence of the military strategy, the achievement of whose goals it is intended to provide. All this necessitates the search for new, more effective ways of solving logistical problems in modern combat. This is true today, including for the Armed Forces of Ukraine. Therefore, the purpose of the work is to consider and implement in practice the logistic support of the state power structures of innovations in the form of a new, improved product - the creation of modular complexes, including various block containers (kitchen-dining room, shower room, laundry, post-office, dispensary and others). They will dramatically change in the future the quality of resolving the issues of everyday life of servicemen during the performance of combat missions in emergency zones and with participation in armed conflicts. Methodology. The methodology is based on the main measures to improve the logistic support system in the near future, including the reorganization of the existing logistic support system and the creation of modular structures of forces and means that are easily adapted and subsequently targeted for specific tasks. Findings. A set of design documentation for the block - container for laundry and shower in the field is offered. Technological modules for combat (block-post), rear (kitchen-dining room), medical (mobile ambulance), and others are also being developed. Originality. One of the peculiarities of reforms carried out in the Armed Forces of Ukraine is their scientific justification. For the theoretical support, development and justification of promising ideas for improving the life support of servicemen during long-term deployment in the field, the use of block containers-mobile multifunctional premises - is proposed for the first time. Practical value. Considering that the military and political leadership of Ukraine considers the logistic support of the troops as one of the components of the all-round support of the armed forces in peacetime and wartime, the use of the proposed ideas for improving the life of the troops will help to form a new image of the material and technical support system of the Armed Forces of Ukraine.

Critical Phenomenology: Borderline Between Grey, Opaque and Non-Transparent Zones – Permanent Transitional Times

In what follows, I will point to theorisations of diagramatic modular models of the human, social and cultural practices that relate to antagonistic and certainly turbulent processing of production and reproduction, political economy, real life, and forms of life in the field of contemporary non-transparent or gray sociality. My main thesis is that the transition has not been completed and that we are now in the midst of transition changes throughout the world – that contemporary media and art fictionalizes or defictionalizes our human condition. My intent in this article is to point to the modular complexity of contemporary phenomena in relation to the criteria of the politics of time (dialectic historicisation) and politics of space (geographic difference). In relation to every contemporaneity that has occurred or is occurring at different times and in different places, contemporary art and culture required different conceptualisations of ‘modernisation’ and different conceptualisations of a critical response to the transition of global/local practices from the margins of society to its hegemonic centre, both internationally and locally. In an epistemological/methodological sense I intend to develop critical phenomenology. Critical phenomenology is a project of the politicization/radicalization of conservative phenomenological thinking.

A simple and efficient algorithm for modeling modular complex networks

In this paper we introduce a new algorithm to generate networks in which node degrees and community sizes can follow any arbitrary distribution. We compare the quality and efficiency of the proposed algorithm and the well-known algorithm by Lancichinetti et al. In contrast to the later one, the new algorithm, at the cost of accuracy, allows to generate two orders of magnitude larger networks in a reasonable time and it can be easily described analytically.

Cullin-RING E3 Ubiquitin Ligases: Bridges to Destruction.

Ubiquitination is a highly conserved post-translational modification in eukaryotes, well known for targeting proteins for degradation by the 26S proteasome. Proteins destined for proteasomal degradation are selected by E3 ubiquitin ligases. Cullin-RING E3 ubiquitin ligases (CRLs) are the largest superfamily of E3 ubiquitin ligases, with over 400 members known in mammals. These modular complexes are tightly regulated in the cell. In this chapter, we highlight recent structural and biochemical advances shedding light on the assembly and architecture of cullin-RING ligases, their dynamic regulation by a variety of host factors, and their manipulation by viral pathogens and small molecules.

Об эффективности функционирования мультикритериального пожарного извещателя

Now detectors that sensing on two and more fire physical factors have appeared and get popular application. These detectors are different concerning usual combined fire detectors and they use more difficult algorithm of information processing. The considered classification of multicriterial fire detectors has allowed to dedicate two features accounting various ways of their technical implementation. The first feature is probably number of used types of monitored physical environment parameters. Now four main channels of detection are actively used in such detectors: heat, smoke, flame and gas. However, this list doesn’t limit possible detected factors of ignition. Now there are known, for example, devices which use acoustic effects, conducting fire, change video image within scene of specter and others. The second feature is way of technical implementation of detection channel. They can be joined by one constructively completed unit or be multiblock structure, with wire communication lines between cooperated components and with fire control panel. Construction complication and the introduction additional connections negatively influence on detector’s reliability. Thus, during evaluating of efficiency it is necessary additionally to consider important indicator - reliability, which can be characterized by degree of readiness of multicriterial fire detector to perform its main function of fire detection at any moment of time. In this article the opportunity of introduction of complex indicator “technical readiness” of multicriterial fire detector is considered. This indicator is probability of performance by multicriterial fire detector the main preset task (objective function), that consists in insured fire detection. The mathematical expression for technical efficiency of multicriterial fire detector is received. Its numeric analysis for determination of dependences character of “technical efficiency” from number of detection channels at different values of detection channels characteristics and parameters their reliability is carried out. The opportunity of construction detector’s optimization by usage of introduced parameter is shown in this article. The offered parameter of technical efficiency may be used in well-founded cases for designing and application of multicriterial fire detector and also for modular complexes and fire alarm systems.

Trs33-Containing TRAPP IV: A Novel Autophagy-Specific Ypt1 GEF.

Ypt/Rab GTPases, key regulators of intracellular trafficking pathways, are activated by guanine-nucleotide exchange factors (GEFs). Here, we identify a novel GEF complex, TRAPP IV, which regulates Ypt1-mediated autophagy. In the yeast Saccharomyces cerevisiae, Ypt1 GTPase is required for the initiation of secretion and autophagy, suggesting that it regulates these two distinct pathways. However, whether these pathways are coordinated by Ypt1 and by what mechanism is still unknown. TRAPP is a conserved modular complex that acts as a Ypt/Rab GEF. Two different TRAPP complexes, TRAPP I and the Trs85-containing TRAPP III, activate Ypt1 in the secretory and autophagic pathways, respectively. Importantly, whereas TRAPP I depletion copies Ypt1 deficiency in secretion, depletion of TRAPP III does not fully copy the autophagy phenotypes of autophagy-specific ypt1 mutations. If GEFs are required for Ypt/Rab function, this discrepancy implies the existence of an additional GEF that activates Ypt1 in autophagy. Trs33, a nonessential TRAPP subunit, was assigned to TRAPP I without functional evidence. We show that in the absence of Trs85, Trs33 is required for Ypt1-mediated autophagy and for the recruitment of core-TRAPP and Ypt1 to the preautophagosomal structure, which marks the onset of autophagy. In addition, Trs33 and Trs85 assemble into distinct TRAPP complexes, and we term the Trs33-containing autophagy-specific complex TRAPP IV. Because TRAPP I is required for Ypt1-mediated secretion, and either TRAPP III or TRAPP IV is required for Ypt1-mediated autophagy, we propose that pathway-specific GEFs activate Ypt1 in secretion and autophagy.

New technologies of 2-D and 3-D modeling for analysis and management of natural resources

For ensuring technological support of research and administrative activity in the sphere of environmental management a specialized modular program complex was developed. The special attention in developing a program complex is focused to creation of convenient and effective tools for creation and visualization 2d and 3D models providing the solution of tasks of the analysis and management of natural resources.

Pressing Technologies for ELVs

This article explains the physical features of various methods of compaction, primarily for end of life vehicle (ELV) bodies coming from ELV processors. By EU directive 2000/53/EC it is mandatory for all EU members to meet the reuse of 95 % of the old vehicle weight. It means that the vehicles have to be decomposed completely – metal shells freed from all the components like interior trim, rubber sealing, glass, all the liquids, seats tanks and other hard metal components like drive-train, engine etc. It follows that pressing machines used for compressing whole vehicles today, are overpowered, with high energy demand, robust structures and heavy weight. Hence it makes sense to concentrate on comparison of pressing methods and choosing the most energy efficient one and the designing a new device, if necessary. This device is aimed for operation in Slovak Republic but also usable in other countries with same or similar requirements such as optimized system for processing of car bodies, mobile concept and modularity of the whole mobile modular disintegration complex consisting of a pressing machine, shears and shredder. Car body structure

Валково-дисковый сепаратор автономного модульного комплекса добычи и переработки торфяного сырья на топливо

Валково-дисковый сепаратор автономного модульного комплекса добычи и переработки торфяного сырья на топливо

Lewis Carroll's Doublets net of English words: network heterogeneity in a complex system.

Lewis Carroll's English word game Doublets is represented as a system of networks with each node being an English word and each connectivity edge confirming that its two ending words are equal in letter length, but different by exactly one letter. We show that this system, which we call the Doublets net, constitutes a complex body of linguistic knowledge concerning English word structure that has computable multiscale features. Distributed morphological, phonological and orthographic constraints and the language's local redundancy are seen at the node level. Phonological communities are seen at the network level. And a balancing act between the language's global efficiency and redundancy is seen at the system level. We develop a new measure of intrinsic node-to-node distance and a computational algorithm, called community geometry, which reveal the implicit multiscale structure within binary networks. Because the Doublets net is a modular complex cognitive system, the community geometry and computable multi-scale structural information may provide a foundation for understanding computational learning in many systems whose network structure has yet to be fully analyzed.

Modular Complexity Analysis for Term Rewriting

All current investigations to analyze the derivational complexity of term rewrite systems are based on a single termination method, possibly preceded by transformations. However, the exclusive use of direct criteria is problematic due to their restricted power. To overcome this limitation the article introduces a modular framework which allows to infer (polynomial) upper bounds on the complexity of term rewrite systems by combining different criteria. Since the fundamental idea is based on relative rewriting, we study how matrix interpretations and match-bounds can be used and extended to measure complexity for relative rewriting, respectively. The modular framework is proved strictly more powerful than the conventional setting. Furthermore, the results have been implemented and experiments show significant gains in power.

Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration

The Spt-Ada-Gcn5-acetyltransferase (SAGA) chromatin-modifying complex possesses acetyltransferase and deubiquitinase activities. Within this modular complex, Ataxin-7 anchors the deubiquitinase activity to the larger complex. Here we identified and characterized Drosophila Ataxin-7 and found that reduction of Ataxin-7 protein results in loss of components from the SAGA complex. In contrast to yeast, where loss of Ataxin-7 inactivates the deubiquitinase and results in increased H2B ubiquitination, loss of Ataxin-7 results in decreased H2B ubiquitination and H3K9 acetylation without affecting other histone marks. Interestingly, the effect on ubiquitination was conserved in human cells, suggesting a novel mechanism regulating histone deubiquitination in higher organisms. Consistent with this mechanism in vivo, we found that a recombinant deubiquitinase module is active in the absence of Ataxin-7 in vitro. When we examined the consequences of reduced Ataxin-7 in vivo, we found that flies exhibited pronounced neural and retinal degeneration, impaired movement, and early lethality.