Shock Model Research Articles

Analyzing the multi-state system under a run shock model

Abstract A system experiences random shocks over time, with two critical levels, d1 and d2, where $d_{1} \lt d_{2}$ . k consecutive shocks with magnitudes between d1 and d2 partially damaging the system, causing it to transition to a lower, partially working state. Shocks with magnitudes above d2 have a catastrophic effect, resulting in complete failure. This theoretical framework gives rise to a multi-state system characterized by an indeterminate quantity of states. When the time between successive shocks follows a phase-type distribution, a detailed analysis of the system’s dynamic reliability properties such as the lifetime of the system, the time it spends in perfect functioning, as well as the total time it spends in partially working states are discussed.

25% HUMAN SERUM ALBUMIN IMPROVES HEMODYNAMICS AND PREVENTS THE NEED FOR NEARLY ALL PREHOSPITAL RESUSCITATION IN A RAT ( RATTUS NORVEGICUS ) MODEL OF TRAUMA AND HEMORRHAGE.

Combat casualty care can be complicated by transport times exceeding the "golden hour," with intervention and resuscitation limited to what the medic can carry. Pharmaceutical albumin comes highly saturated with nonesterified fatty acids (NEFAs). We recently showed that treatment with 25% bovine serum albumin (BSA) loaded with oleic acid, but not NEFA-free BSA, improved survival for hours after severe hemorrhage and often eliminated the need for resuscitation in rats. However, it was unknown whether pharmaceutical albumin, derived from human sources and loaded with caprylic acid (CA), would have the same benefits. We compared adjunct treatment with oleic acid-saturated BSA, CA-saturated BSA, pharmaceutical human serum albumin, or a no-albumin control in a similar rat hemorrhagic shock model to determine whether the three NEFA-albumin groups provided the same benefits relative to control. We found almost no significant differences among the NEFA-albumin groups in any measure. Mortality in controls was too low to allow for detection of improvement in survival, but NEFA-albumin groups had significantly improved hemodynamics, lactate clearance, and greatly reduced fluid requirements compared with controls. Contrary to expectations of "dehydration," 25% albumins shifted little additional fluid into the vasculature. Rather, they restored protein to the autotransfusion fluid. Nonesterified fatty acids-albumin did not worsen lung permeability, but we observed a loss of circulating protein suggesting it may have increased overall vascular permeability. Our findings suggest that, though imperfect, 25% human serum albumin could be a solution for resuscitation in austere conditions requiring prolonged field care.

Near-infrared spectroscopy for kidney oxygen monitoring in a porcine model of hemorrhagic shock, hemodilution, and REBOA

Acute kidney injury is a common complication of trauma and hemorrhagic shock. In a porcine model of hemorrhagic shock, resuscitative endovascular balloon aortic occlusion (REBOA) and hemodilution, we hypothesized that invasive kidney oxygen concentration measurements would correlate more strongly with noninvasive near infra-red spectroscopy (NIRS) oxygen saturation measurements when cutaneous sensors were placed over the kidney under ultrasound guidance compared to placement over the thigh muscle and subcutaneous tissue. Eight anesthetized swine underwent hemorrhagic shock 4 of which were resuscitated with intravenous fluids prior to the return of shed blood (Hemodilution protocol) and 4 of which underwent REBOA prior to resuscitation and return of shed blood (REBOA protocol). There was a moderate correlation between the NIRS and kidney tissue oxygen measurements (r = 0.61 p < 0.001; r = 0.67 p < 0.001; r = 0.66 p < 0.001for left kidney, right kidney, and thigh NIRS respectively). When the animals were separated by protocol, the Hemodilution group showed a weak or nonsignificant correlation between NIRS and kidney tissue oxygen measurements (r = 0.10 p < 0.001; r = 0.01 p = 0.1007; r = 0.28 p < 0.001 for left kidney, right kidney, and thigh NIRS respectively). This contrasts with the REBOA group, where left and right kidney as well as thigh NIRS were moderately correlated with kidney tissue oxygen (r = 0.71 p < 0.001; r = 0.74 p < 0.001; r = 0.70 p < 0.001; for left kidney, right kidney, and thigh NIRS respectively). There was a strong correlation between both kidney NIRS signals and thigh NIRS measurements (r = 0.85 p < 0.001; r = 0.88 p < 0.001;for left kidney vs thigh and right kidney vs thigh respectively). There was also a strong correlation between left and right kidney NIRS (r = 0.90 p < 0.001). These relationships were maintained regardless of the resuscitation protocol. These results suggest that kidney NIRS measurements were more closely related to thigh NIRS measurements than invasive kidney tissue oxygen concentration.

VX-765 Alleviates Circadian Rhythm Disorder in a Rodent Model of Traumatic Brain Injury Plus Hemorrhagic Shock and Resuscitation.

Severe traumatic brain injury (TBI) can result in persistent complications, including circadian rhythm disorder, that substantially affect not only the injured people, but also the mood and social interactions with the family and the community. Pyroptosis in GFAP-positive astrocytes plays a vital role in inflammatory changes post-TBI. We determined whether VX-765, a low molecular weight caspase-1 inhibitor, has potential therapeutic value against astrocytic inflammation and pyroptosis in a rodent model of TBI plus hemorrhagic shock and resuscitation (HSR). A weight-drop plus bleeding and refusion model was used to establish traumatic exposure in rats. VX-765 (50mg/kg) was injected via the femoral vein after resuscitation. Wheel-running activity was assessed, brain magnetic resonance images were evaluated, the expression of pyroptosis-associated molecules including cleaved caspase-1, gasdermin D (GSDMD), and interleukin-18 (IL-18) in astrocytes in the region of anterior hypothalamus, were explored 30days post-trauma. VX-765-treated rats had significant improvement in circadian rhythm disorder, decreased mean diffusivity (MD) and mean kurtosis (MK), increased fractional anisotropy (FA), an elevated number and branches of astrocytes, and lower cleaved caspase-1, GSDMD, and IL-18 expression in astrocytes than TBI + HSR-treated rats. These results demonstrated that inhibition of pyroptosis-associated astrocytic activations in the anterior hypothalamus using VX-765 may ameliorate circadian rhythm disorder after trauma. In conclusion, we suggest that interventions targeting caspase-1-induced astrocytic pyroptosis by VX-765 are promising strategies to alleviate circadian rhythm disorder post-TBI.

CLASSY. VIII. Exploring the Source of Ionization with UV Interstellar Medium Diagnostics in Local High-z Analogs* *Based on observations made with the NASA/ESA Hubble Space Telescope, obtained from the Data Archive at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-26555.

In the current JWST era, rest-frame UV spectra play a crucial role in enhancing our understanding of the interstellar medium (ISM) and stellar properties of the first galaxies in the epoch of reionization (z > 6). Here, we compare well-known and reliable optical diagrams sensitive to the main ionization source (i.e., star formation, SF; active galactic nuclei, AGN; and shocks) to UV counterparts proposed in the literature—the so-called “UV–BPT diagrams”—using the HST COS Legacy Archive Spectroscopic SurveY (CLASSY), which is the largest high-quality, high-resolution, and broad-wavelength range atlas of far-UV spectra for 45 local star-forming galaxies. In particular, we explore where CLASSY UV line ratios are located in the different UV diagnostic plots, taking into account state-of-the-art photoionization and shock models, and, for the first time, the measured ISM and stellar properties (e.g., gas-phase metallicity, ionization parameter, carbon abundance, and stellar age). We find that the combination of C iii] λ λ1907,9 He ii λ1640 and O iii] λ1666 can be a powerful tool to separate between SF, shocks, and AGN at subsolar metallicities. We also confirm that alternative diagrams without O iii] λ1666 still allow us to define an SF-locus, with some caveats. Diagrams including C iv λ λ1548,51 should be taken with caution given the complexity of this doublet profile. Finally, we present a discussion detailing the ISM conditions required to detect UV emission lines, visible only in low gas-phase metallicity (12 + log(O/H) ≲ 8.3) and high ionization parameter (log(U) ≳ −2.5) environments. Overall, CLASSY and our UV toolkit will be crucial in interpreting the spectra of the earliest galaxies that JWST is currently revealing.

GRB 200612A: An Ultralong Gamma-Ray Burst Powered by Magnetar Spinning Down

GRB 200612A could be classified as an ultralong gamma-ray burst due to its prompt emission lasting up to ∼1020 s and the true timescale of the central engine activity t burst ≥ 4 × 104 s. The late X-ray light curve with a decay index of α = 7.53 is steeper than the steepest possible decay from an external shock model. We propose that this X-ray afterglow can be driven by dipolar radiation from the magnetar spindown during its early stage, while the magnetar collapsed into the black hole before its spindown, resulting in a very steep decay of the late X-ray light curve. The optical data show that the light curve is still rising after 1.1 ks, suggesting a late onset. We show that GRB 200612A’s optical afterglow light curve is fitted with the forward shock model by Gaussian structured off-axis jet. This is a special case among GRBs, as it may be an ultralong gamma-ray burst powered by a magnetar in an off-axis observation scenario.

A Novel Pre-Clinical Modeling of Massive Hemorrhagic Shock in Non-Human Primate: A Safe and Reproducible Method (Macaca fascicularis)

Although non-human primates are the closest animals to humans to simulate physiological and metabolic responses, there is a paucity of primate hemorrhagic shock models that are standardized and reproducible. Herein, we describe a model that is a clinical replica of extreme class IV hemorrhagic shock with a step-by-step description of the procedure in cynomolgus macaque monkeys. The physiological changes that occurred during the process were evaluated using hemodynamic parameters, echocardiogram, and laboratory values. Five female monkeys were subjected to trauma laparotomy, followed by cannulation of the abdominal aorta to achieve graded hemorrhage. A central line was placed in the right internal jugular vein, which was subsequently used for laboratory sampling and volume resuscitation. The withdrawal of blood was ceased when a predefined cardiac endpoint with cardiac arrhythmia or bradycardia was reached. The animals were then immediately resuscitated with transfusion. The primary cardiac endpoint was consistently reached in all 5 animals during the fourth hemorrhage when more than 70% of the estimated total blood volume was lost. No mortality occurred during the process. The blood pressure, cardiac output measured from an echocardiogram, and hemoglobin correlated well with increasing loss of circulating volume, whereas the pulse pressure variation did not. The echocardiogram was also a useful predictor for urgent volume replacement. This model offers a safe and reproducible surgical hemorrhagic model in non-human primates and simulates clinical practice. This could provide a useful platform on which further studies can be carried out to address unanswered questions in trauma management.

Criss-cross Nebula: A Case Study of Shock Regions with Resolved Microstructures at Scales of ∼1000 au

Using integral field spectroscopy (IFS) from MaNGA, we study the resolved microstructures in a shocked region in the Criss-cross Nebula (CCN), with an unprecedentedly high resolution of ≲1000 au. We measure surface brightness maps for 34 emission lines, which can be broadly divided into three categories: (1) the [O iii] λ5007-like group including seven high-ionization lines and two [O ii] auroral lines that uniformly present a remarkable lane structure, (2) the Hα λ6563-like group, including 23 low-ionization or recombination lines that present a clump-like structure, and (3) [O ii] λ3726 and [O ii] λ3729 showing high densities at both the [O iii] λ5007 lane and the Hα clump. We use these measurements to constrain resolved shock models implemented in MAPPINGS V. We find our data can be reasonably well fitted by a model that includes a plane-parallel shock with a velocity of 133 ± 5 km s −1, plus an isotropic two-dimensional Gaussian component, which is likely another clump of gas ionized by photons from the shocked region, and a constant background. We compare the electron density and temperature profiles as predicted by our model with those calculated using observed emission-line ratios. We find different line ratios to provide inconsistent temperature maps, and the discrepancies can be attributed to observational effects caused by limited spatial resolution and projection of the shock geometry, as well as contamination of the additional Gaussian component. Implications on shock properties and perspectives on future IFS-based studies of the CCN are discussed.

Particle acceleration test with Cas A multiwavelength emission

AbstractThe investigation of supernova remnants (SNRs) across the electromagnetic spectrum from radio up to very high energy gamma‐rays can serve as a test of the particle acceleration and touches on one of the unresolved problems of modern astrophysics, namely the origin of cosmic rays and the Galaxy's contribution to the overall cosmic ray spectrum. The multiwavelength observations of Cas A SNR demonstrated that structure and spectral features have clear signs of young SNRs and its overall properties make this object the best target to test a hypothesis of cosmic ray origin in SNRs. Studies of Cas A at very high energies by SHALON telescope showed the location of TeV gamma‐ray emission region relative to the position of reveres shock. Also, the spectral energy distribution was obtained at high and very high energies. To describe the spectral and structural features of this SNR viewed in non‐thermal emission, two approaches involving reverse and also both reverse and forward shocks to the mechanism of diffusive shock acceleration of cosmic rays in Cas A were applied. It is demonstrated that the observational properties of Cas A are well reproduced by the hadronic model with significant contribution of both the forward and reverse shocks in the generation of broadband emission. Calculation results suggest that the very high efficiency of particle acceleration in Cas A, which value is up to 25% of the supernova explosion energy with energy of accelerated particles not exceeding of eV. Whereas, the forward shock model predicts the spectral characteristics of the TeV‐gamma‐emission corresponding to ones detected at 800 GeV–40 TeV that are the evidence of acceleration of the hadronic cosmic rays in shells of SNRs up to eV

The GUAPOS project: G31.41+0.31 Unbiased ALMA sPectral Observational Survey

Context. The astrochemistry of the important biogenic element phosphorus (P) is still poorly understood, but observational evidence indicates that P-bearing molecules are likely associated with shocks. Aims. We study P-bearing molecules and some shock tracers towards one of the chemically richest hot molecular cores, G31.41+0.31, in the framework of the project “G31.41+0.31 Unbiased ALMA sPectral Observational Survey” (GUAPOS), which is being carried out with the Atacama Large Millimeter Array (ALMA). Methods. We observed the molecules PN, PO, SO, SO2, SiO, and SiS through their rotational lines in the spectral range 84.05– 115.91 GHz covered by the GUAPOS project. Results. PN is clearly detected, while PO is tentatively detected. The PN emission arises from two regions southwest of the hot core peak, named regions 1 and 2 here, and is undetected or tentatively detected towards the hot core peak. The PN and SiO lines are very similar both in spatial emission morphology and spectral shape. Region 1 is partly overlapping with the hot core and is warmer than region 2, which is well separated from the hot core and located along the outflows identified in previous studies. The SO, SO2, and SiS emissions are also detected towards the PN-emitting regions 1 and 2, but arise mostly from the hot core. Moreover, the column density ratio SiO/PN remains constant in regions 1 and 2, while SO/PN, SiS/PN, and SO2/PN decrease by about an order of magnitude from region 1 to region 2, indicating that SiO and PN have a common origin even in regions with different physical conditions. The PO/PN ratio in region 2, where PO is tentatively detected, is ~0.6–0.9, which is in line with the predictions of pure shock models. Conclusions. Our study provides robust confirmation of previous observational evidence that PN emission is tightly associated with SiO and is likely a product of shock chemistry, as the lack of a clear detection of PN towards the hot core allows us to rule out relevant formation pathways in hot gas. We propose the PN-emitting region 2 as a new astrophysical laboratory for shock-chemistry studies.

Discovery of a Collimated Jet from the Low-luminosity Protostar IRAS 16253−2429 in a Quiescent Accretion Phase with the JWST

Investigating Protostellar Accretion (IPA) is a JWST Cycle 1 GO program that uses NIRSpec integral field units and MIRI Medium Resolution Spectrograph to obtain 2.9–28 μm spectral cubes of young, deeply embedded protostars with luminosities of 0.2–10,000 L ⊙ and central masses of 0.15–12 M ⊙. In this Letter, we report the discovery of a highly collimated atomic jet from the Class 0 protostar IRAS 16253−2429, the lowest-luminosity source (L bol = 0.2 L ⊙) in the IPA program. The collimated jet is detected in multiple [Fe ii] lines and [Ne ii], [Ni ii], and H i lines but not in molecular emission. The atomic jet has a velocity of about 169 ± 15 km s−1, after correcting for inclination. The width of the jet increases with distance from the central protostar from 23 to 60 au, corresponding to an opening angle of 2.°6 ± 0.°5. By comparing the measured flux ratios of various fine-structure lines to those predicted by simple shock models, we derive a shock speed of 54 km s−1 and a preshock density of 2.0 × 103 cm−3 at the base of the jet. From these quantities and using a suite of jet models and extinction laws, we compute a mass-loss rate between 0.4 and 1.1 ×10−10 M ⊙ yr −1. The low mass-loss rate is consistent with simultaneous measurements of low mass accretion rate (2.4 ± 0.8 × 10−9 M ⊙ yr−1) for IRAS 16253−2429 from JWST observations, indicating that the protostar is in a quiescent accretion phase. Our results demonstrate that very low-mass protostars can drive highly collimated, atomic jets, even during the quiescent phase.

Sub-GeV Gamma Rays from Nearby Seyfert Galaxies and Implications for Coronal Neutrino Emission

Recent observations of high-energy neutrinos by IceCube and gamma rays by the Fermi Large Area Telescope (LAT) and the MAGIC telescope have suggested that neutrinos are produced in gamma-ray opaque environments in the vicinity of supermassive black holes. In this work, we present 20 MeV–1 TeV spectra of three Seyfert galaxies whose nuclei are predicted to be active in neutrinos, NGC 4151, NGC 4945, and the Circinus galaxy, using 14.4 yr of Fermi LAT data. In particular, we find evidence of sub-GeV excess emission that can be attributed to gamma rays from NGC 4945, as was also seen in NGC 1068. These spectral features are consistent with predictions of the magnetically powered corona model, and we argue that NGC 4945 is among the brightest neutrino active galaxies detectable for KM3Net and Baikal-GVD. On the other hand, in contrast to other reported results, we do not detect gamma rays from NGC 4151, which constrains neutrino emission from the accretion shock model. Future neutrino detectors such as IceCube-Gen2 and MeV gamma-ray telescopes such as AMEGO-X will be crucial for discriminating among the theoretical models.

A 3D Parametric Venusian Bow Shock Model with the Effects of Mach Number and Interplanetary Magnetic Field

Using global magnetohydrodynamics simulations, we have developed a three-dimensional parametric model for the Venusian bow shock based on a generalized conic section function defined by six parameters, with the effects of the solar wind magnetosonic Mach number (M MS) and the interplanetary magnetic field (IMF) involved. The parametric model’s results reveal the following findings: (1) The size of the Venusian bow shock is primarily determined by M MS. An increase in M MS results in the bow shock moving closer to Venus and a reduction in its flaring angle. (2) Both the subsolar standoff distance and the bow shock’s flaring angle increase with the strength of the IMF components that are perpendicular to the solar wind flow direction (B Y and B Z in the Venus-centered solar orbital coordinate system), whereas the parallel IMF component (B X ) has a limited impact on the subsolar standoff distance but affects the flaring angle. (3) The cross section of the bow shock is elongated in the direction perpendicular to the IMF on the Y–Z plane, and the elongation degree is enhanced with increasing intensities of B Y and B Z . (4) The quasi-parallel bow shock locates closer to the planet as compared to the quasi-perpendicular bow shock. These findings are in alignment with prior empirical and theoretical models. The influences of M MS and IMF on the bow shock’s position and geometry are attributed to the propagation of fast magnetosonic waves, showing the nature of the formation of a collisionless bow shock under the interaction of magnetized flow with an atmospheric object.

In quest of China sports lottery development path to common prosperity in 2035.

The China sports lottery contributes to sports and welfare causes. This study aims to construct a macro forecasting model supporting its sustained growth aligned with Vision 2035. The modeling employed a distributional regression. Sales data of the China sports lottery from 2011 to 2022 were chosen as the response variable, alongside various macro- and event-level explanatory factors. A gamma distribution best fit the data. In the stable model spanning 2011-2019, urbanization, population dynamics, and FIFA emerged as significant contributors (Chi-square p < 0.05) to the location shift parameter. These three factors retained their significance in the 2011-2022 shock model, where shock itself notably impacted sales (p < 0.001). Utilizing the shock model, we simulated the trajectory of the China sports lottery up to 2035. China's demographics changes are poised to create structural headwinds starting in 2026, leading to an anticipated decline in sales driven by population shifts from 2032 onward. However, the FIFA effect is projected to continue fortifying this sector. Beyond offering original insights into the sales trajectory until 2035, specifically concerning new urbanization, negative population growth, and the FIFA effect, this macro forecasting framework can assist in addressing the policy priority of balancing growth with risk mitigation. We recommend policymakers connect market development with mass sports, potentially garnering a dual boost from the growing population of older consumers and the inherent benefits of a "FIFA (mass sports)" effect. A people-centered approach to the China sports lottery could significantly contribute to the long-range objectives of achieving common prosperity outlined in Vision 2035.

Systemic Hemodynamics, Cardiac Mechanics, and Signaling Pathways Induced by Extracorporeal Membrane Oxygenation in a Cardiogenic Shock Model.

Peripheral venoarterial extracorporeal membrane oxygenation (VA-ECMO) is increasingly being used in patients suffering from refractory cardiogenic shock (CS). Although considered life-saving, peripheral VA-ECMO may also be responsible for intracardiac hemodynamic changes, including left ventricular overload and dysfunction. Venoarterial extracorporeal membrane oxygenation may also increase myocardial wall stress and stroke work, possibly affecting the cellular cardioprotective and apoptosis signaling pathways, and thus the infarct size. To test this hypothesis, we investigated the effects of increasing the peripheral VA-ECMO blood flow (25-100% of the baseline cardiac output) on systemic and cardiac hemodynamics in a closed-chest CS model. Upon completion of the experiment, the hearts were removed for assessment of infarct size, histology, apoptosis measurements, and phosphorylation statuses of p38 and protein Kinase B (Akt), and extracellular signal-regulated kinase mitogen-activated protein kinases (ERK-MAPK). Peripheral VA-ECMO restored systemic perfusion but induced a significant and blood flow-dependent increase in left ventricular preload and afterload. Venoarterial extracorporeal membrane oxygenation did not affect infarct size but significantly decreased p38-MAPK phosphorylation and cardiac myocyte apoptosis in the border zone.

13C-Metabolic flux analysis detected a hyperoxemia-induced reduction of tricarboxylic acid cycle metabolism in granulocytes during two models of porcine acute subdural hematoma and hemorrhagic shock.

Supplementation with increased inspired oxygen fractions has been suggested to alleviate the harmful effects of tissue hypoxia during hemorrhagic shock (HS) and traumatic brain injury. However, the utility of therapeutic hyperoxia in critical care is disputed to this day as controversial evidence is available regarding its efficacy. Furthermore, in contrast to its hypoxic counterpart, the effect of hyperoxia on the metabolism of circulating immune cells remains ambiguous. Both stimulating and detrimental effects are possible; the former by providing necessary oxygen supply, the latter by generation of excessive amounts of reactive oxygen species (ROS). To uncover the potential impact of increased oxygen fractions on circulating immune cells during intensive care, we have performed a 13C-metabolic flux analysis (MFA) on PBMCs and granulocytes isolated from two long-term, resuscitated models of combined acute subdural hematoma (ASDH) and HS in pigs with and without cardiovascular comorbidity. Swine underwent resuscitation after 2 h of ASDH and HS up to a maximum of 48 h after HS. Animals received normoxemia (PaO2 = 80 - 120 mmHg) or targeted hyperoxemia (PaO2 = 200 - 250 mmHg for 24 h after treatment initiation, thereafter PaO2 as in the control group). Blood was drawn at time points T1 = after instrumentation, T2 = 24 h post ASDH and HS, and T3 = 48h post ASDH and HS. PBMCs and granulocytes were isolated from whole blood to perform electron spin resonance spectroscopy, high resolution respirometry and 13C-MFA. For the latter, we utilized a parallel tracer approach with 1,2-13C2 glucose, U-13C glucose, and U-13C glutamine, which covered essential pathways of glucose and glutamine metabolism and supplied redundant data for robust Bayesian estimation. Gas chromatography-mass spectrometry further provided multiple fragments of metabolites which yielded additional labeling information. We obtained precise estimations of the fluxes, their joint credibility intervals, and their relations, and characterized common metabolic patterns with principal component analysis (PCA). 13C-MFA indicated a hyperoxia-mediated reduction in tricarboxylic acid (TCA) cycle activity in circulating granulocytes which encompassed fluxes of glutamine uptake, TCA cycle, and oxaloacetate/aspartate supply for biosynthetic processes. We further detected elevated superoxide levels in the swine strain characterized by a hypercholesterolemic phenotype. PCA revealed cell type-specific behavioral patterns of metabolic adaptation in response to ASDH and HS that acted irrespective of swine strains or treatment group. In a model of resuscitated porcine ASDH and HS, we saw that ventilation with increased inspiratory O2 concentrations (PaO2 = 200 - 250 mmHg for 24 h after treatment initiation) did not impact mitochondrial respiration of PBMCs or granulocytes. However, Bayesian 13C-MFA results indicated a reduction in TCA cycle activity in granulocytes compared to cells exposed to normoxemia in the same time period. This change in metabolism did not seem to affect granulocytes' ability to perform phagocytosis or produce superoxide radicals.

Circulating biomarkers of the CS4P and CLIP scores are not altered in a pig model of acute cardiogenic shock and additional short-term circulatory support

BackgroundCardiogenic shock (CS) is the leading cause of death in patients with myocardial infarction with a mortality rate greater than 50%. Recently, the CS 4 Proteins (CS4P) and CLIP scores have been developed to predict survival in CS patients. However, their impact in acute CS and additional short-term left ventricular (LV) circulatory support as prognostic markers is currently not known. Methods and resultsCS was induced in a porcine model by injecting microsphere particles into the left main coronary artery. Mechanical circulatory support was performed by additional percutaneous LV unloading using an Impella microaxial flow-pump for 30 minutes. Serum samples were collected at baseline, following the onset of CS, and additional LV unloading. Serum levels of biomarkers of the CS4P (beta-2-microglobulin, ALDOB, L-FABP, SerpinG1) and the CLIP scores (Cystatin C, Lactate, Interleukin-6, NT-proBNP) were neither different at any time point investigated nor did they correlate with cardiac output. ConclusionThe CS4P and CLIP scores do not reflect immediate whole-body dysregulation in acute CS and have not been able to predict the potential reversal following additional short-term mechanical support by LV unloading in our experimental model. The impact of both scores as prognostic markers after the immediate onset of CS and following additional short-term LV unloading to identify patients at greatest risk remains to be determined.

Allergenic protein-induced type I hypersensitivity models: a review.

Type I hypersensitivity affects approximately one-third of the global population. As the pathophysiology underlying the development of type I hypersensitivity (asthma, food allergy, and anaphylactic shock, etc.) is complex and heterogeneous, animal model studies continue to be the key to identifying novel molecular pathways and providing therapeutic strategies. Selection of the animal model should be done with careful consideration of the protocol variables, animal species, and strains to accurately reflect the clinical symptoms typical of humans. The following databases were searched: PubMed and Web of Science. Foreign allergens include allergenic proteins and chemical haptens. This review summarizes the various methods used for designing animal models of common allergenic protein-induced type I hypersensitivity, namely, passive anaphylaxis model, active systemic anaphylaxis/anaphylaxis shock model, food allergy model, asthma model, and IgE-mediated cell models. Additionally, we summarize shrimp tropomyosin-induced type I hypersensitivity models from our previous studies and discuss their advantages and limitations compared with that of ovalbumin-induced models.

Machine Learning Predictive Model for Septic Shock in Acute Pancreatitis with Sepsis.

Acute pancreatitis (AP) progresses to septic shock can be fatal. Early identification of high-risk patients and timely intervention can prevent and interrupt septic shock. By analyzing the clinical characteristics of AP with sepsis, this study uses machine learning (ML) to build a model for early prediction of septic shock within 28 days of admission, which guided emergency physicians in resource allocation and medical decision-making. This retrospective cohort study collected data from the emergency departments (EDs) of three tertiary care hospitals in China. The dataset was randomly divided into a training dataset (70%) and a testing dataset (30%). Ten ML classifiers were utilized to analyze characteristics of AP with sepsis in the training dataset upon admission. Results were evaluated through cross-validation analysis. The optimal model was then tested on the testing dataset without any parameter modifications. The ML model was evaluated using the receiver operating characteristic curve (ROC) and compared to scoring systems through the DeLong test. A total of 604 AP patients with sepsis were included in this study. The auto-encoder (AE) model based on mean normalization, Pearson correlation coefficient (PCC), and recursive feature elimination (RFE) selection, achieved the highest Area Under the Curve (AUC) on the validation dataset (AUC 0.900, accuracy 0.868), with the AUC of 0.879 and accuracy of 0.790 on the testing dataset. Compared to the Sequential Organ Failure Assessment (AUC 0.741), quick Sequential Organ Failure Assessment (AUC 0.727), Acute Physiology and Chronic Health Evaluation II (AUC 0.778), and Bedside Index of Severity in Acute Pancreatitis (AUC 0.691), the AE model showed superior performance. The AE model outperforms traditional scoring systems in predicting septic shock in AP patients with sepsis within 28 days of admission. This assists emergency physicians in identifying high-risk patients early and making timely medical decisions.

Magnetic field properties inside the jet of Mrk 421

Aims. We aim to probe the magnetic field geometry and particle acceleration mechanism in the relativistic jets of supermassive black holes. Methods. We conducted a polarimetry campaign from radio to X-ray wavelengths of the high-synchrotron-peak (HSP) blazar Mrk 421, including Imaging X-ray Polarimetry Explorer (IXPE) measurements from 2022 December 6–8. During the IXPE observation, we also monitored Mrk 421 using Swift-XRT and obtained a single observation with XMM-Newton to improve the X-ray spectral analysis. The time-averaged X-ray polarization was determined consistently using the event-by-event Stokes parameter analysis, spectropolarimetric fit, and maximum likelihood methods. We examined the polarization variability over both time and energy, the former via analysis of IXPE data obtained over a time span of 7 months. Results. We detected X-ray polarization of Mrk 421 with a degree of ΠX = 14 ± 1% and an electric-vector position angle ψX = 107 ± 3° in the 2–8 keV band. From the time variability analysis, we find a significant episodic variation in ψX. During the 7 months from the first IXPE pointing of Mrk 421 in 2022 May, ψX varied in the range 0° to 180°, while ΠX remained relatively constant within ∼10–15%. Furthermore, a swing in ψX in 2022 June was accompanied by simultaneous spectral variations. The results of the multiwavelength polarimetry show that ΠX was generally ∼2–3 times greater than Π at longer wavelengths, while ψ fluctuated. Additionally, based on radio, infrared, and optical polarimetry, we find that the rotation of ψ occurred in the opposite direction with respect to the rotation of ψX and over longer timescales at similar epochs. Conclusions. The polarization behavior observed across multiple wavelengths is consistent with previous IXPE findings for HSP blazars. This result favors the energy-stratified shock model developed to explain variable emission in relativistic jets. We considered two versions of the model, one with linear and the other with radial stratification geometry, to explain the rotation of ψX. The accompanying spectral variation during the ψX rotation can be explained by a fluctuation in the physical conditions, for example in the energy distribution of relativistic electrons. The opposite rotation direction of ψ between the X-ray and longer wavelength polarization accentuates the conclusion that the X-ray emitting region is spatially separated from that at longer wavelengths. Moreover, we identify a highly polarized knot of radio emission moving down the parsec-scale jet during the episode of ψX rotation, although it is unclear whether there is any connection between the two events.