Miniaturized Matrix Solid-phase Dispersion Research Articles

Optimization of a modified Captiva EMR-lipid method based on micro-matrix solid-phase dispersion coupled with gas chromatography-mass spectrometry for the determination of nine bisphenols in mussel samples.

This work describes a reliable, cheap, easy and fast method for analysis of nine bisphenols in mussel samples by gas chromatography-mass spectrometry after trimethylsilylation. The modified method consisted of miniaturized matrix solid phase dispersion (micro-MSPD) in a glass Pasteur pipette using Captiva EMR (enhanced matrix removal)-lipid as the sorbent. Good linearity was obtained in the work range (1-500 μg L-1) with a correlation coefficient (R2) ≥ 0.998. The method accuracy and precision were determined at two concentration levels. The results show recoveries ranging from 55 to 111%. The precision varied from 1.95 to 11.4% (RSD). The whole quantification limits were between 0.056 and 3.42 μg per kg dry weight. The analytical procedure was applied for the analyses of five mussel samples collected from Galician Rias. The major compound was BPA, and wild mussels from Rías de Ferrol, Vigo and A Coruña had the highest levels. The proposed method is suitable for the analysis of BPA and its analogues in mussel samples.

Miniaturized matrix solid-phase dispersion assisted by deep eutectic solvent for acrylamide determination in bread samples.

Acrylamide determination is important to state its quantity in baked food preventing any potential carcinogenic effects. Matrix solid-phase dispersion (MSPD) extraction is an extraction procedure based on a homogenization phase between a solid sample and a solid dispersing material to break sample increasing analyte extraction yield, often used for acrylamide determination. The addition of a green deep eutectic solvent (DES) during the MSPD homogenization phase improves the analyte extraction, giving the possibility to reduce the amount of organic solvent used. In this work, a miniaturized MSPD extraction assisted by a DES was developed to determine acrylamide in bread, using high-performance liquid chromatography coupled with mass spectrometry detection. The optimized procedure provides 1:1 (w/w) matrix-to-dispersing material ratio, 2mL of methanol as extraction solvent, and 50 μL of choline chloride-glycerol DES added during the homogenization phase. Method validation ensured good results with minimum recoveries of 90%, high precision with a maximum intra-day error of 4%, and inter-day error of 6%. Limit of detection and limit of quantification resulted to be 16μg/kg and 35μg/kg, respectively. This miniaturized extraction procedure represents a good alternative to those reported in the literature, guaranteeing great performance and respecting green chemistry principles.

Optimization of an analytical method based on miniaturized matrix solid-phase dispersion combined with gas chromatography-mass spectrometry for the determination of bisphenols in mussel samples

In this study, a new method has been optimized to determinate nine bisphenols (BPs) in mussel samples. This approach is based on miniaturized matrix solid-phase dispersion combined with gas chromatography-mass spectrometry determination after trimethylsilylation with 99% N,O-Bis-(trimethylsilyl)trifluoroacetamide + 1 % trimethylchlorosilane. The optimization process investigated the derivatization (derivatizing agent volume, time and temperature) and sample preparation (C18 co-column packed and C18 dispersant agent amounts and elution volume) parameters. A second clean-up step was performed using EMR-Lipid cartridges in order to efficiently remove lipids from mussel, the optimized parameters were Captiva EMR-Lipid sorbent amount and elution volume. The whole method was validated and very good linearity (r2 > 0.99) was obtained. The recoveries (accuracy) at two concentration levels ranged from 51.2 to 109 % and the coefficients of variation (precision) were between 0.55 and 13 %. The limits of quantification were between 0.014 μg/Kg dry weight for BPM and 4.0 μg/Kg dry weight for BPS. The analytical procedure was applied to four mussel samples from Galician Rías. Except for one of four samples whose BPAF level was below the limit of quantification, all BPs were found in concentrations that ranged from 0.39 to 301 μg/Kg dry weight.

Miniaturized matrix solid-phase dispersion and solid-phase clear-up combined with capillary electrophoresis for efficient determination of trace bioactive components in complicated sample matrix: Take Wubi Shanyao Pill as an example.

Accurate quantitative analysis of trace analytes in a complicated matrix is a challenge in modern analytical chemistry. An appropriate analytical method is considered to be one of the most common gaps during the whole process. In this study, a green and efficient strategy based on miniaturized matrix solid-phase dispersion and solid-phase extraction combined with capillary electrophoresis was first proposed for extracting, purifying and determining target analytes from complicated matrix, using Wubi Shanyao Pill as an example. In detail, 60mg of samples were dispersed on MCM-48 to obtain high yields of analytes, then the extract was purified with a solid-phase extraction cartridge. Finally, four analytes in the purified sample solution were determined by capillary electrophoresis. The parameters affecting the extraction efficiency of matrix solid-phase dispersion, purification efficiency of solid-phase extraction and separation effect of capillary electrophoresis were investigated. Under the optimized conditions, all analytes demonstrated satisfactory linearity (R2 >0.9983). What's more, the superior green potential of the developed method for the determination of complex samples was confirmed by the Analytical GREEnness Metric Approach. The established method was successfully applied in the accurate determination of target analytes in Wubi Shanyao Pill and thus provided reliable, sensitive, and efficient strategy support for its quality control.

Multi-residue method to determine selected personal care products from five classes in fish based on miniaturized matrix solid-phase dispersion and solid-phase microextraction coupled to gas chromatography-mass spectrometry

A method featuring matrix solid-phase dispersion combined with solid-phase microextraction coupled to gas chromatography-mass spectrometry was developed to determine parabens, musks, antimicrobials, UV filters, and an insect repellent in fish. Optimization and validation of the method was carried out on tilapia and salmon samples. Acceptable linearity (R2 > 0.97), precision (relative standard deviations < 13 %) and accuracy (recovery > 80 %) at two concentration levels for all analytes were obtained using both matrices. The limits of detection ranged from 0.01 to 1.01 μg g−1 (wet weight) for all analytes except for methyl paraben. The SPME Arrow format was applied to increase the sensitivity of the method, and yielded detection limits more than ten times lower than those achieved with traditional SPME. The miniaturized method can be applied to various fish species, regardless of their lipid content, and represents a useful tool for quality control and food safety purposes.

Isolation of DNA from plant tissues using a miniaturized matrix solid-phase dispersion approach featuring ionic liquid and magnetic ionic liquid solvents

The isolation of high-quality plant genomic DNA is a major prerequisite in many plant biomolecular analyses involving nucleic acid amplification. Conventional plant cell lysis and DNA extraction methods involve lengthy sample preparation procedures that often require large amounts of sample and chemicals, high temperatures and multiple liquid transfer steps which can introduce challenges for high throughput applications. In this study, a simple, rapid, miniaturized ionic liquid (IL)-based extraction method was developed for the isolation of genomic DNA from milligram fragments of Arabidopsis thaliana plant tissue. This method is based on a modification of vortex-assisted matrix solid-phase dispersion (VA-MSPD) in which the trihexyl(tetradecyl)phosphonium bis(trifluoromethylsulfonyl)imide ([P6,6,6,14+][NTf2-]) IL or trihexyl(tetradecyl)phosphonium tris(hexafluoroacetylaceto)nickelate(II) ([P6,6,6,14+][Ni(hfacac)3-]) magnetic IL (MIL) was directly applied to treated plant tissue (∼1.5 mg) and dispersed in an agate mortar to facilitate plant cell lysis and DNA extraction, followed by recovery of the mixture with a qPCR compatible co-solvent. This study represents the first approach to use ILs and MILs in a MSPD procedure to facilitate plant cell lysis and DNA extraction. The DNA-enriched IL- and MIL-cosolvent mixtures were directly integrated into the qPCR buffer without inhibiting the reaction while also circumventing the need for additional purification steps prior to DNA amplification. Under optimum conditions, the IL and MIL yielded 2.87 ± 0.28 and 1.97 ± 0.59 ng of DNA/mg of plant tissue, respectively. Furthermore, the mild extraction conditions used in the method enabled plant DNA in IL- and MIL-cosolvent mixtures to be preserved from degradation at room temperature.

Solid‐phase adsorption methodologies of naturally occurring anthraquinones: A review

Solid-phase extraction applied to plant matrices is nowadays a well-validated technique allowing to concentrate and purify different secondary metabolites. Several classes of phytochemicals have been selectively extracted by this methodology. During the last decade attention has been focused on biologically active anthraquinones from numerous sources like edible, healthy, and medicinal plants. The aim of this review is to provide a detailed literature survey of the solid-phase adsorption methodologies for the extraction of natural anthraquinones reported so far and to discuss and propose future directions in this field of research. Substructure search was performed in the SciFinder Scholar, PubMed, Medline, and Scopus databases. The first report about application of solid-phase adsorption for the purification of anthraquinones appeared in the literature in 2002. From this date, and in particular during recent years, the most notable examples included the use of chitin- and chitosan-based polymers, of molecularly imprinted polymers, of coated magnetic nanoparticles, of miniaturized matrix solid-phase dispersion, of functionalized resins, of differently structured lamellar solids, and finally of vortex-synchronized matrix solid-phase dispersion. The herein detailed solid-phase adsorption methodologies are powerful tools to selectively extract natural anthraquinones and/or provide anthraquinone-enriched phytopreparations. Nevertheless, many other important methods have been applied to synthetic anthraquinones (e.g., azo dyes). These could be conveniently employed also for natural anthranoids. Studies in this field are discussed in this review article.

Miniaturized ternary deep eutectic solvent-based matrix solid-phase dispersion: A green sample preparation method for the determination of chlorophenols in river sediment.

New ternary deep eutectic solvents were prepared and applied as efficient green dispersing solvents in miniaturized matrix solid-phase dispersion to extract chlorophenols from river sediments for the first time. High-performance liquid chromatography coupled with a photodiode array detector was used to analyze the target analytes. The significant factors affecting the extraction were optimized as follows: dispersant (100mg), sample (100mg), ternary eutectic solvents (150μl), grinding for 1min, 450μl of acetonitrile as the elution solvent, and vortex mixing for 20s. Under the optimal conditions, the method exhibited excellent linearity (correlation coefficient>0.9980), low limits of detection between 1.039-2.478μg/g, and extraction recoveries between 93.9% and 99.2%. Furthermore, the method demonstrated excellent precision in the intra- and inter-day analysis with a relative standard deviation below 6%. When compared to conventional extraction techniques, the miniaturized matrix solid-phase dispersion considerably reduced samples and solvent usag, offering important environmental benefits. The green profile of the method was assessed using the complementary green analytical procedure index tool confirming its eco-friendship. The technique was finally employed to evaluate sediment samples from three distinct locations along the Zuibaiji River, indicating its applicability for monitoring environmental samples.

Synchronous microextraction of active and toxic compounds from medicinal plant using nano-SiO2 assisted miniaturized matrix solid-phase dispersion

The technique of nano-SiO2-assisted miniaturized matrix solid-phase dispersion (MMSPD) was successfully developed and applied for the first time in synchronous microextraction, concentration, and separation of active and toxic ingredients from Corydalis Rhizoma. Nano-SiO2 was served as a sorbent in the MMSPD, providing a larger specific surface area and better hydrophilicity, resulting in more efficient extraction of the target compounds. The MMSPD mixture was obtained by grinding 10 mg Corydalis Rhizoma, the standard solution of toxic compounds, and nano-SiO2 (40 mg) for 60 s. The sample mixture was then eluted with MeOH + 4 % (v/v) formic acid (pH = 4.8) elution solution. The analysis of sample extracts were performed by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The response surface methodology predicted that the optimum amount of sorbent, the grinding time, and the pH of the elution solvent were 40.11 mg, 60.34 s, and 4.74, respectively. The technique was validated by the methodology and was able to obtain good linearity (R2 > 0.992) as well as a low detection limit (0.05 to 3.00 ng mL−1). Meanwhile, the precision of the method was verified with interday precisions (RSD%, n = 6) in the range of 1.7–4.4 % for peak area. The result of the sample analysis indicated that trace amounts of propazine, trietazine, and simazine were detected in the Corydalis Rhizoma sample, and the content of tetrahydropalmatine is 0.82 μg mg−1. The extraction recoveries ranged from 80.3 % to 109.6 %. This effective microextraction method is suitable for synchronous extraction of isoquinoline alkaloids and triazine herbicides from Corydalis Rhizoma samples.

A miniaturized matrix solid-phase dispersion methodology (µMSPD) for determination of β-carboline alkaloids in tobacco samples by UPLC-ESI-Q-TOF/MSE

Alkaloids play an important role in the chemical composition of tobacco, due to their effects that have led to the global consumption of this commodity. The β-carboline alkaloids present inhibitory action against the enzyme monoamine oxidase (MAO), which enhances the susceptibility to chemical dependence in smokers. There is a need for scientific studies to ensure the correct identification and quantification of these compounds in tobacco matrices. In this work, we present the development and validation of a microextraction analytical method for determination and quantification of the alkaloids harmaline, harmalol, harmane, harmine, norharmane, and tetrahydroharmine in natural and processed samples of tobacco, employing micro-matrix solid-phase dispersion (μMSPD), ultra-performance liquid chromatography (UPLC), and mass spectrometry (MS). The optimized μMSPD procedure employed of 0.01 g of sample, 0.1 g of Discovery® DPA-6S adsorbent, and elution with 2 mL of aqueous 1 % formic acid solution, resulting in a fast, practical, economical, and environmentally friendly technique. Validation of the methodology showed that it presented good linearity (R2 > 0.9945), satisfactory accuracy and precision (in the range from 72 ± 16 % to 109 ± 9 %), and limits of quantification (LOQ) and detection (LOD) in the ranges 0.02–1.0 µg g−1 and 0.01–0.2 µg g−1, respectively. The developed method was applied to tobacco samples, proving to be efficient for determination of β-carboline alkaloids. The compounds harmane and norharmane were quantified in samples of fresh tobacco leaves, cured tobacco leaves, twisted tobacco, and cigarettes. Harmine was only not quantified in the cigarettes.

Fast determination of phthalates in mussel samples by micro-matrix solid-phase dispersion (micro-MSPD) coupled with GC\u2013MS/MS

A fast, effective and low cost sample preparation method based on miniaturized matrix solid-phase dispersion (micro-MSPD) combined with gas chromatography coupled to tandem triple-quadrupole-mass spectrometry (GC–MS/MS) has been developed for the determination of six phthalate diesters (DMP, DEP, DBP, BzBP, DEHP and DnOP) in mussel samples. The six target compounds have been included in the list of priority pollutants by United States Environmental Protection Agency. The extraction step was optimized on real spiked mussel coming from Galician Rías by means of a factorial design. The final procedure involved the use of 0.45 g of sample, 0.5 g of dispersant agent (Florisil) and 3 mL of organic solvent (ethyl acetate). The optimized method was validated giving satisfactory analytical performance, low detection limits (0.09 to 6.73 ng g−1 dw) and high recoveries (93 and 114%). The validated method was applied to four real mussel samples coming from Galician Rías.

Development and Optimization of a Method for Organochlorine Pesticides Determination in Mussels Based on Miniaturized Matrix Solid-Phase Dispersion Combined with Gas Chromatography–Tandem Mass Spectrometry

Development and Optimization of a Method for Organochlorine Pesticides Determination in Mussels Based on Miniaturized Matrix Solid-Phase Dispersion Combined with Gas Chromatography–Tandem Mass Spectrometry

Miniaturized Matrix Solid-Phase Dispersion for the Analysis of Ultraviolet Filters and Other Cosmetic Ingredients in Personal Care Products

A method based on micro-matrix solid-phase dispersion (μ-MSPD) followed by gas-chromatography tandem mass spectrometry (GC–MS/MS) was developed to analyze UV filters in personal care products. It is the first time that MSPD is employed to extract UV filters from cosmetics samples. This technique provides efficient and low-cost extractions, and allows performing extraction and clean-up in one step, which is one of their main advantages. The amount of sample employed was only 0.1 g and the extraction procedure was performed preparing the sample-sorbent column in a glass Pasteur pipette instead of the classic plastic columns in order to avoid plastizicer contamination. Factors affecting the process such as type of sorbent, and amount and type of elution solvent were studied by a factorial design. The method was validated and extended to other families of cosmetic ingredients such as fragrance allergens, preservatives, plasticizers and synthetic musks, including a total of 78 target analytes. Recovery studies in real sample at several concentration levels were also performed. Finally, the green extraction methodology was applied to the analysis of real cosmetic samples of different nature.

Miniaturized matrix solid-phase dispersion based on deep eutectic solvent and carbon nitride associated with high-performance liquid chromatography: A new feasibility for extraction and determination of trace nitrotoluene pollutants in soil samples

In this research, a miniaturized version of matrix solid phase dispersion (MSPD) method was developed for successful extraction of five nitrotoluenes from soil samples. The terpene based deep eutectic solvent (DES) composed of borneol and DL-menthol was used simultaneously as extraction solvent and dispersing liquid. Graphitic-carbon nitride (g-C3N4) as sorbent was prepared by pyrolysis of melamine at a muffle furnace. The MSPD mixture was obtained by grinding soil, DES, and g-C3N4. Minimum amount of acetonitrile was used in the elution step. Some experimental variables including type and volume of DES, type of dispersant, mass ratio of dispersant to sample, volume of elution solvent, grinding time, and ultrasonication time were evaluated. Analysis of extracted samples was performed by high performance liquid chromatography-ultra violet detection. Under the optimized conditions; the limits of detection were achieved in the range of 0.12−0.33 μg g−1, and the relative standard deviations were equal to or lower than 9.3%. The extraction recoveries were ranged from 78 to 96%. This straightforward and fast method is effective for environmentally friendly extraction of nitrotoluenes from soil samples.

Column packing elimination in matrix solid phase dispersion by using water compatible magnetic molecularly imprinted polymer for recognition of melamine from milk samples

This paper reports a new variant of sample clean-up procedure based on miniaturized matrix solid phase dispersion (MSPD) combining with water compatible magnetic molecularly imprinted polymers (MMIP) for preconcentration of melamine from milk samples prior high-performance liquid chromatography with UV detection. By employing MMIP as dispersant sorbent matrix interferences could be eliminated completely and the column packing step was interestingly omitted that decreasing in glassware expenditure, labor effort and extraction time which cause improvement of the method performance. In order to investigate the functionality, morphology and magnetic properties of the prepared MMIP, it was fully characterized by FT-IR, VSM, SEM, and TEM. Multivariate optimization was applied as a versatile statistical approach for evaluating the influence of the main variables and their interactions on extraction efficiency. Under optimized conditions, the proposed method exhibited a wide linear response in the concentration range of 250.0–5000.0 μg L−1, satisfactory recoveries for all samples (86.6% ≤), and supreme repeatability (RSD less than 6.3%) were achieved as well. The low limit of detection close to 67.0 μg L−1 implied high ability of proposed method for isolation of melamine from complicate milk sample. These befitting results demonstrated that the developed method is unique in terms of the facility, rapidly and least solvent consumption as well as accurate analysis of melamine at trace levels.

Quantification of eight active ingredients in crude and processed radix polygoni multiflori applying miniaturized matrix solid-phase dispersion microextraction followed by UHPLC.

A rapid, efficient, and green sample preparation method has been developed to extract eight active ingredients (gallic acid, catechins, epicatechin, polydatin, 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside, resveratrol, emodin, and physcion) in radix polygoni multiflori by miniaturized matrix solid-phase dispersion microextraction. Simple and sensitive ultra high performance liquid chromatography combined with ultraviolet detection has been applied to analyze the multiple compounds. The best results were obtained by adding 25mg sample into 25mg adsorbent and grinding for 2min with disorganized silica as adsorbent and 1mL 150mM 1-dodecyl-3-methylimidazolium bromide as a green eluting solvent. Good linearity (r2 >0.998) for each analyte was obtained by this method. The intra-day and inter-day precision (RSD) were both below 5.31%, and the recoveries of the analytes ranged from 93.3 to 100.0%. This simple miniaturized matrix solid-phase dispersion microextraction method for analyzing the compounds in radix polygoni multiflori needs a short time and requires little sample and reagent. Thus, this method is far more eco-friendly and efficient than traditional extraction methods (reflux and ultrasound-assisted extraction). The present investigation provided a promising method for the fast preparation and discrimination of chemical differences in crude and processed radix polygoni multiflori.

Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid-phase dispersion extraction coupled with ultra high-performance liquid chromatography with photodiode array detection and quadrupole time-of-flight mass spectrometry.

A simple and effective sample preparation process based on miniaturized matrix solid-phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5-dicaffeoylqunic acid, 1,5-dicaffeoylqunic acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol-3-O-rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenanthraquinones (cryptotanshinone), and furans (5-hydroxymethylfurfural) in Naoxintong capsule by ultra high-performance liquid chromatography. The optimized condition was that 25mg Naoxintong powder was blended homogeneously with 100mg Florisil PR for 4min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.

Miniaturized matrix solid-phase dispersion followed by liquid chromatography-tandem mass spectrometry for the quantification of synthetic dyes in cosmetics and foodstuffs used or consumed by children

Miniaturized matrix solid-phase dispersion (MSPD) followed by liquid chromatography tandem mass spectrometry (LC–MS/MS) has been proposed for the simultaneous analysis of different classes of synthetic dyes in confectionery and cosmetics intended for or mostly consumed by children. Selected compounds include most of the permitted dyes as food additives as well as some of the most frequently used to color cosmetic products in accordance with the respective European directives. MSPD procedure was optimized by means of experimental design, allowing an effective, rapid and simple extraction of dyes with low sample and reagents consumption (0.1g of sample and 2mL of elution solvent). LC–MS/MS was optimized for good resolution, selectivity and sensitivity using a low ionic strength mobile phase (3mM NH4Ac-methanol). Method performance was demonstrated in real samples showing good linearity (R≥0.9928) and intra- and inter-day precision (%RSD≤15%). Method LODs were ≤0.952μgg−1 and ≤0.476μgg−1 for confectionery and cosmetic samples, respectively. Recoveries of compounds from nine different matrices were quantitative. The validated method was successfully applied to 24 commercial samples (14 cosmetics and 10 foods) in which 9 of the selected dyes were found at concentrations up to 989μgg−1, exceeding in some cases the regulated maximum permitted limits. A non-permitted dye, Acid Orange 7, was found in one candy.

Simultaneous microextraction of inorganic iodine and iodinated amino acids by miniaturized matrix solid-phase dispersion with molecular sieves and ionic liquids

This study presents an effective method of using miniaturized matrix solid phase dispersion (MSPD) for the microextraction of inorganic iodine and iodinated amino acids from seaweed samples. Quantification of the target analytes was performed by ultrahigh performance liquid chromatography with UV detection. Molecular sieve (SBA-15) was chosen as the dispersing adsorbent with an ionic liquid (1-dodecyl-3-methylimidazolium bromide) as the elution solvent. The experimental conditions for the MSPD, such as the type of sorbent, ratio of sorbent to sample, type and concentration of the elution solvent, and grinding time were evaluated and optimized. Under the final working conditions, good recoveries were obtained in the range of 86.5–95.4%, with relative standard deviation values below 6.0% in all cases. The limits of detection and limits of quantitation were in the ranges of 3.7–16.7ng/mL and 12.4ng/mL, respectively. Compared with common ultrasound assisted extraction, the advantages of this green approach are low consumption of the sorbent and solvent, short extraction time and good selectivity, even in complicated matrices. The proposed SBA-15-based MSPD method was successfully applied to the microextraction of potassium iodide, 3-iodo-l-tyrosine, and 3,5-diiodo-l-tyrosine from kelp, nori and undaria pinnatifida, respectively.

Application of ionic liquids for elution of bioactive flavonoid glycosides from lime fruit by miniaturized matrix solid-phase dispersion

In this work, two flavonoid glycosides (neohesperidin and naringin) in lime fruit were effectively extracted by miniaturized matrix solid phase dispersion (MSPD), followed by ultra-performance liquid chromatography-ultraviolet detection. The best results were obtained using Florisil (150mg) as the sorbent and 1-butyl-3-methylimidazolium tetrafluoroborate (0.4mL, 250mM) as the elution solvent. This work represents the first attempt of using ionic liquids as a green eluent for extraction of the investigated compounds in miniaturized MSPD. Compared with the conventional methods, the proposed method is advantageous due to improved enrichment factor and reduced reagent consumption. A good linearity was observed with r2 values (>0.998). Meanwhile, the method gave acceptable recoveries (90.16–96.47%) for the determination of flavonoids in plant samples. The limits of detection of the two analytes ranged between 4.08 and 5.04μg/g. The results showed that the optimized method has a great potential for sample preparation in routine analysis of complex plant samples.