MTA Fillapex Research Articles

Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers.

ObjectivesThis study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus.Materials and MethodsBC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05).ResultsThe MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control (p < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control (p > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound (p < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex (p < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing (p > 0.05).ConclusionsAll tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.

Effect of two irrigation protocols and obturation techniques on the apical sealing ability of different root canal sealers: An in vitro study

Abstract Aim: The aim of this study was to compare the influence of two irrigation protocols and obturation techniques on the apical seal of three endodontic sealers. Materials and Methods: Crowns of 130 single-rooted teeth were cut and 10 roots were selected as controls, and the remaining roots were divided into four experimental groups (n = 30) according to the type of irrigation protocol and obturation technique. Each group was divided into three subgroups (n = 10) according to the type of root canal sealer. All root canals were prepared with size F4 Protaper Universal and irrigated with 3% sodium hypochlorite (NaOCl) + 3% hydrogen peroxide (H2O2) (Groups 1 and 3) or 3% NaOCl + 2% chlorhexidine (CHX) (Groups 2 and 4). Two gutta-percha obturation techniques were used: single-cone (Groups 1 and 2) and continuous wave (Groups 3 and 4) techniques. The following sealers were tested: MTA Fillapex (Subgroup A), AH Plus (Subgroup B), and Realseal SE (Subgroup C). The apical seal was assessed using dye penetration method, and the data were statistically analyzed. Results: In the first group, AH Plus showed the best apical seal (0.46 ± 0.098 mm), while RealSeal SE system showed the worst apical seal (3.47 ± 0.434 mm). In the second group, MTA Fillapex (0.58 ± 0.115 mm) and AH Plus (0.63 ± 0.109 mm) showed the best apical seal. In the third and fourth groups, all types of sealers showed similar high leakage patterns. Marked increase in the apical seal of RealSeal was observed in the third group (1.71 ± 0.453 mm). Conclusions: The type of irrigation protocol and obturation technique could affect the apical seal of tested sealers. When the single-cone technique was used, AH Plus with NaOCl + H2O2 and MTA Fillapex with NaOCl + CHX irrigation protocols showed significantly a high apical seal. RealSeal SE showed the worst apical seal when canals were irrigated with NaOCl/H2O2 and filled with the single-cone technique. All sealers showed inferior results when used with the continuous wave technique.

Evaluation of the film thickness and antibacterial property of mineral trioxide aggregate mixed with propylene glycol as a root canal sealer

The aim of this in vitro study was to compare the film thickness and antibacterial properties of mineral trioxide aggregate-propylene glycol (MTA-PG) as a sealer in comparison with MTA Fillapex and AH26 sealers. In these in vitro study the antibacterial property of the sealers was evaluated using direct contact test in fresh and set states. Enterococcus faecalis was incubated in direct contact with fresh and set materials. The growth of exposed bacteria was evaluated by counting colony-forming units (CFUs) after 10 min and 1 h in the culture medium. The film thickness of sealers was measured according to the International Standard Organization 6876/2012. The data were statistically analyzed using an independent t-test and repeated measures of ANOVA. The level of significance was set at 0.05. CFU means in AH26 was significantly more than other groups (P < 0.0001), but there was no difference between MTA-PG and MTA Fillapex. The mean of CFUs in set AH26 after 1 h exposure was significantly >10-min exposure (P = 0.006). The mean film thickness values of MTA Fillapex, MTA-PG, and AH26 were 57.3, 50.9, and 78.3 μm, respectively. MTA-Fillapex and MTA-PG showed distinct antibacterial effect. AH26 showed more antibacterial effect in fresh state in comparison with set state. The film thickness of MTA-PG and MTA-Fillapex was significantly less than AH26.

Dentin moisture conditions strongly influence its interactions with bioactive root canal sealers.

ObjectivesIt is known that bioactive materials interact with the dentin to undergo biomineralization. The exact role of moisture in this interaction is unknown. Here, we investigate the effects of dentin moisture conditions on the dislocation resistance of two bioactive root canal sealers (MTA Fillapex [Angelus Solucoes Odontologicas] and GuttaFlow BioSeal [Colténe/Whaledent AG]) at 3 weeks and 3 months after obturation.Materials and MethodsMandibular premolars (n = 120) were prepared and randomly divided into 3 groups based on the dentin condition: group 1, dry dentin; group 2, moist dentin; group 3, wet dentin. Each group was divided into 2 subgroups for root canal filling: MTA Fillapex and GuttaFlow BioSeal. Dislocation resistance was evaluated by measuring the push-out bond strength at 3 weeks and 3 months. Failure modes were examined under a stereomicroscope. Data were statistically analyzed by Kruskal-Wallis test with a significance level of 5%.ResultsMoist dentin resulted in higher bond strength values for both materials at both time points. This was significantly higher than wet and dry dentin for both the sealers at the 3 months (p < 0.05), while at 3 weeks it was significant only for GuttaFlow Bioseal. The different moisture conditions demonstrated similar trends in their effects on the dislocation resistance of the 2 root canal sealers.ConclusionsThe dentin moisture conditions had a significant impact on its interaction with the bioactive materials tested. Maintaining moist dentin, but not dry or wet dentin, may be advantageous before the filling root canals with bioactive sealers.

Comparison of the effect of sodium hypochlorite, EDTA, and etidronic acid on wettability of root canal sealers using contact angle goniometer: An in vitro study

Aim: To evaluate and compare the effect of Sodium hypochlorite, etidronic acid, and ethylenediamine tetraacetic acid (EDTA) on the contact angle of AH Plus and MTA Fillapex root canal sealers. Materials and Methods: 45 freshly extracted single rooted teeth were selected and decoronated. They were then split longitudinally to obtain two halves which were trimmed as flat dentin bars of 2 mm thickness. They were randomly assigned to 3 groups of 30 each. The samples were surface treated with 2.5% sodium hypochlorite in Group A, 2.5% sodium hypochlorite followed by 17% EDTA in Group B, and a combination of 2.5% sodium hypochlorite and 18% etidronic acid in Group C. Each group was subdivided into two subgroups based on the sealer used, i.e., Subgroup 1-AH Plus and Subgroup 2-MTA Fillapex. The contact angle was analyzed using Rame Harte Goniometer. Results: AH Plus has significantly lesser contact angles than MTA Fillapex. The contact angle values showed the order of C1 Conclusion: Irrigating solutions affect the wettability of rootcanal sealers. AH Plus showed better wettability compared to MTA Fillapex. Irrigation with a combination of sodium hypochlorite and etidronic acid significantly decreased the contact angle, thereby increasing the wettability of sealers.

Postoperative pain after root canal filling with different endodontic sealers: a randomized clinical trial.

The aim of this randomized clinical trial was to compare the occurrence and intensity of postoperative pain and analgesic intake after root canal treatment, using different root canal sealers. Sixty single-rooted teeth diagnosed with asymptomatic necrosis and apical periodontitis were randomly assigned to 3 experimental groups (n=20), according to the root canal sealer: AH Plus, Endofill or MTA Fillapex. Endodontic treatment was performed in two sessions, and calcium hydroxide was used as the intracanal dressing. Patients were instructed to record pain intensity as none, slight, moderate and severe. Scores from 1 to 4 were attributed to each level of pain after 24 h, 48 h and 7 days. The need for analgesic intake was also recorded. Differences in the incidence of postoperative pain and the need for an analgesic were analyzed using the chi-square test. Differences in pain intensity after treatment were analyzed using the ordinal (linear) chi-square test. No significant differences were detected among the groups in terms of either incidence or intensity of postoperative pain, or need for analgesic intake, at any timepoint (p>0.05). No pain was reported after 7 days. AH Plus, Endofill and MTA Fillapex used for filling root canals resulted in the same rate of postoperative pain and need for analgesic medication.

Bacterial Leakage of Four Endodontic Sealers Using Fresh Human Saliva.

Bacterial leakage plays a considerable role in endodontic related failures. The aim of this study was to compare the bacterial leakage of bioceramic (including Well Root ST and MTA Fillapex) and resin-based root canal sealers (naming AH-Plus and AdSeal). Sixty-eight maxillary central incisors were included in this experimental study. These were randomly allocated into four experimental groups, which were obturated with gutta-percha and four different sealers (n=15), and the two control groups (positive, n=4 and negative, n=4). A dual-chamber technique was carried out using fresh human saliva and traced for 90 days. The Chi-square and Long Rank tests were performed for data analysis. Bacterial leakage was observed in 33.3% of the samples in Well Root ST, 40% in AH-Plus group, 53.3% in AdSeal group, and 66.6% in MTA Fillapex group. The difference between the Well Root ST and MTA Fillapex groups was significant (P=0.02). The mean leakage time between the Well Root ST (85.6 days) and MTA Fillapex groups (71.2 days) was also significant (P=0.037). The results of this in vitro study revealed bacterial leakage in all experimental groups. The Well Root ST and the MTA Fillapex groups showed the lowest and highest bacterial leakages, respectively. Then it seems that the Well Root ST is a good substitute for MTA Fillapex in clinical applications.

Root Fracture Resistance of Maxillary Premolars Obturated with Three Root Canal Sealers after Passive Utrasonic Irrigation: An in Vitro study.

Maxillary premolars, may be more susceptible to fracture due to their anatomy; especially when there is loss of tooth structure. Therefore, it is necessary to evaluate materials and techniques that may increase fracture resistance during and post root canal treatment. This in vitro study aimed to evaluate root fracture resistance of maxillary premolars when filled with three root canal sealers as well as whether this resistance would be increased by passive ultrasonic irrigation (PUI). Sixty-four maxillary premolars with two roots were randomly divided into one negative control group (intact canals; n = 8), one positive control group (instrumented, unsealed canals; n = 8), and six experimental groups (n = 8), which were instrumented with ProTaper Next rotary system up to X2 file and subdivided according to final irrigation (with or without PUI) and type of sealer used (AH-Plus [AH], MTA Fillapex [MTA], or EndoSequence BC Sealer [ES]). The specimens were subjected to fracture strength test in a universal testing machine at a speed of 1 mm/min until fracture. The maximum force required to induce fracture was recorded (N). The Kruskal-Wallis test and DUNN test were used for analysis. The lowest force required to cause root fracture was observed in the positive control group (310.48 ± 54.08 N); this was significantly different from the other groups (P < 0.05). There was no significant difference between experimental groups obturated with the same sealer, whether with or without PUI (AH with PUI: 558.80 ± 87.12 N; AH without PUI: 508.75 ± 97.55 N; MTA with PUI: 507.27 ± 174.55 N; MTA without PUI: 516.69 ± 96.56 N; ES with PUI: 526.76 ± 143.97 N; ES without PUI: 628.40 ± 94.74 N) (P > 0.05). There was also no significant difference between the experimental groups and the negative control group (P > 0.05). In this in vitro study PUI did not increase the fracture resistance of maxillary premolars, while AH Plus, MTA Fillapex, EndoSequence sealers increased fracture resistance of instrumented root canals.

Comparative evaluation of sealer penetration depth into radicular dentinal tubules using confocal scanning microscope: an in vitrostudy

Background: Endodontic treatment involves the removal of the vital and necrotic contents of the root canal through chemo-mechanical means followed by obturation of the prepared root canal to prevent the ingress of fluids and avoid bacterial infection or regrowth. Root canal sealers and core filling materials are used together to fill the irregularities in the root. Penetration into the dentinal tubules also results in the inhibition of bacterial regrowth and increases the success of root canal therapy. Aim: This study aimed to evaluate the penetration depth of various sealers into the dentinal tubules using a confocal microscope. Materials and methods: A total of 65 specimens were decoronated to standardize the root length of 13mm. Working length was determined, and Biomechanical preparation for all the samples was done with a rotary ProTaper file till F4. Samples were randomly divided into five groups containing 13 teeth in each group based on the sealer used, namely Group 1: Endomethasone (n=13), Group 2: AH-Plus (n=13), Group 3: Roekoseal (n=13), Group 4: MTA Fillapex (n=13), Group 5: Endosequence BC (n=13). All the sealers were labelled with Rhodamine-B dye, and samples were obturated using cold lateral compaction technique. The specimens were sectioned orthogonally at coronal, middle, and apical thirds. All the samples were examined with a Zeiss Pascal Laser Scanning Microscope to examine the sealer penetration depth into the dentinal tubules. The data were subjected to statistical analysis using one- way Analysis of Variance (ANOVA) and Tukey's Honest Significant Difference (HSD) tests. Results: Endosequence BC showed the highest penetration into dentinal tubules, followed by MTA Fillapex and Roekoseal, AH-Plus, and Endomethasone exhibited the least penetration. Conclusion: Endosequence BC sealer exhibited maximum penetration. All the groups showed maximum penetration at coronal third, followed by the middle and apical third.

Push-Out Bond Strength of MTA as Root Canal Sealer: A Systematic Review

Introduction: Endodontic treatment includes different phases like controlling the growth of microorganism, thorough cleaning and shaping and fluid-tight seal. Sealer plays an important role in Root Canal Treatment (RCT). Sealer fills the space between filling material and dentin. Sealer also acts as a lubricant. Sealer has ability to fill the spaces and prevent microleakege from the root canal. Mineral Trioxide Aggregate (MTA) is a calcium silicate based sealer which has high radiopacity, low solubility and good antibacterial activity. Aim: To evaluate push-out bond strength of MTA as a root canal sealer. Materials and Methods: Studies which were published in English, studies with different years of publication were included. Studies which includes bond strength of MTA as Endodontic Sealer to dentinal wall were also included. Studies used in this review were in-vitro studies and clinical trials. Studies showing tissue toxicity, biocompatibility of MTA were excluded. Also, case reports and in-vivo studies were excluded. All abstracts as well as full texts were studied. PubMed/Medline, Web of Science and Scopus were used for this study. Keywords used were “root canal sealer”, “push-out bond strength”, “MTA”. Total 18 articles were collected. But only five articles fulfilled the inclusion and exclusion criteria. So only five articles were included in this study and discussed in detail. Results: According to the results, of articles which were chosen for this study stated that MTA can be used as an endodontic sealer though it shows lesser push-out bond strength when compared with different newer sealers. Conclusion: In this systematic review, only one in-vitro study showed that Proroot MTA used as root canal sealer exhibits high push-out bond strength than other sealers like MTA Fillapex sealer and AH plus sealer. While MTA Fillapex show lesser push-out bond strength in comparison with other sealers like Proroot MTA sealer, AH plus, Endo CPM sealer. So this systematic review partially fulfills the hypothesis as it gives a utility of Proroot MTA as a root canal sealer.

Evaluation of Sealing Ability of Three Root Canal Sealers: An In Vitro Study

To evaluate the sealing ability of three different types of sealers using confocal laser microscopy. Sixty extracted single-root premolars were selected and divided into three groups (20 teeth in each group) according to the type of sealer used, namely, mineral trioxide aggregate (MTA) Fillapex, AH Plus, and Bio C Sealer. Root canal preparation and obturation were done in all the samples. Roots was dissected transversely in apical plane. Percentage of gap from region to canal circumference was calculated using a confocal laser microscope. Samples were subjected to statistical analysis. High dye penetration was seen with AH Plus compared to MTA Fillapex and least with Bio C Sealer. The AH Plus is the best sealer with respect to seal ability of all the three. This study helps to appraise the sealing ability of the different types of sealers using confocal laser microscopy which is useful for the success of root canal treatment. As sealer has to seal voids, foramina, and canals, it should have good penetration for the success of root canal treatment.

Comparative evaluation of flow rate of four different endodontic sealers: An in vitro study

Aim: The purpose of this study was to compare the flow abilities of four different root canal sealers namely, AH Plus, mineral trioxide aggregate (MTA) Fillapex, Sealapex, and Adseal. Materials and Methods: According to the method proposed by International Organization for Standardization (ISO) specification 6876/2012 (root canal sealing materials), a volume of 0.05 ml of the cement was taken and mixed according to the manufacturer's recommendation and placed on a glass plate. At 180 ± 5 s after mixing, the second glass plate of 20 g was placed on top of the sealer, followed by the weight of mass 100 g to make a total mass on the plate of 120 ± 2 g. After 10 min from the start of mixing, the weight was removed and the diameter of the compressed disc of sealer was measured. The mean of five such values for each sealer was taken as the flow of the material. Results: The flow obtained for the sealers are as follows: 21.84 mm for Adseal, 20.01 mm for AH Plus, 22.43 mm for MTA Fillapex, and 19.84 mm for Sealapex. The results were expressed as arithmetic means, and the statistical analysis was then performed for the mean values using ANOVA and Tukey's tests using SPSS 16.0 and a value of Conclusion: All the sealers tested conform to ISO specification that requires that a sealer should have a diameter of not

Comparative Evaluation of Antifungal Efficacy of Five Root Canal Sealers against Clinical Isolates of Candida albicans: A Microbiological Study.

ABSTRACTAim and objectiveThe aim of this study was to evaluate and compare the antifungal efficacy of MTA Fillapex, Metapex, zinc oxide eugenol cement, Endomethasone, and Endoflas against Candida albicans.Materials and methodsRoot canal exudates of 30 patients were tested against MTA Fillapex (Angelus), Metapex (BioMed), zinc oxide eugenol (Deepak Enterprise), Endomethasone (Septodont), Endoflas FS (Sanlor Laboratories), MTA (Angelus) (positive control), and glycerine (negative control). Children with failed endodontic cases were included in the study. Tube dilution and agar diffusion methods were used to check the antifungal efficacy of the root canal sealers. In tube dilution method, 24-well culture plates containing freshly mixed material along with Candida albicans were used. Wells containing MTA (Angelus) along with Sabouraud dextrose agar and Candida albicans served as positive control while glycerine along with Sabouraud dextrose agar and Candida albicans served as negative control. All plates were incubated at 37°C for 24 hours. Growth of the fungi was monitored after 24 hours by the presence of the turbidity. The samples were recultured to test the experimental material using agar well diffusion method, and the Petri plates were incubated for 24 hours and 72 hours. Zone of inhibition was measured after respective time period. Paired t test was used for the data analysis.ResultsIt was seen in tube dilution method Endomethasone showed least turbidity while maximum was shown by Metapex; similar results were seen in case of agar well diffusion method in which largest zone of inhibition was shown by Endomethasone while smallest was by Metapex.ConclusionIt was concluded that Endomethasone showed maximum efficacy against Candida albicans as compared to Metapex.How to cite this articleSingh S, Srivastava B, Gupta K, et al. Comparative Evaluation of Antifungal Efficacy of Five Root Canal Sealers against Clinical Isolates of Candida albicans: A Microbiological Study. Int J Clin Pediatr Dent 2020;13(2):119–123.

To compare and evaluate the antimicrobial activity of three different root canal sealers: An In Vitro Study.

Aim of the Study:The purpose of this study is to compare and analyze the antimicrobial activity of three different root canal sealers.Materials and Methods:In this study, the antimicrobial activity of three different root canal sealers were compared against two strains of bacteria Enterococcus faecalis which is known to be common isolates of necrotic pulp and endodontic lesions. This test was done at various time intervals (1, 6, 15, and 60 min) using agar diffusion test and direct contact test.Results:Bioceramic showed the best antimicrobial activity against E. faecalis among the three groups of sealers used. Moreover, the results were statistically analyzed.Conclusion:Within the limitations of this study, it can be concluded that Bioceramic sealer showed the best antimicrobial activity followed by MTA Fillapex and Apexit.

External root surface temperature changes during high-temperature injectable thermoplasticized root canal obturation in simulated immature teeth

Introduction: The present study evaluated the change in the temperature of the external root surface of simulated immature teeth obturated with high-temperature injectable thermoplasticized gutta-percha. Materials and Methods: Root canals of 60 mandibular premolars were enlarged till size # 6 peeso freely passed through the apex. After placement of an apical barrier of 4 mm of mineral trioxide aggregate (MTA), two K-type thermocouples were attached at middle and apical thirds. The canals were obturated using high-temperature injectable thermoplasticized gutta-percha with either no sealer; AH plus sealer; or MTA Fillapex sealer. Maximum temperature rise was measured and evaluated. The data were analyzed using two-way ANOVA test. Results: The temperature increased after 4 s of placement of heated gutta-percha. There was no significant effect of sealer placement on the peak rise in temperature at the middle third (P Conclusions: The use of thermoplasticized gutta-percha can significantly increase the temperatures on the external root surface.

Ageing of TotalFill BC Sealer and MTA Fillapex in Simulated Body Fluid.

Objective:The bioactivities of TotalFill BC and the MTA Fillapex sealers were evaluated.Methods:Sixty horizontal root sections were enlarged to size 5 Gates Glidden and randomly divided into six groups (n=10 in each group). In Groups 1–3, sections were filled with TFBCS, while sections in groups 4–6 were filled with MTAFS. Specimens from groups 1 and 4 were soaked in simulated body fluid (SBF) for one day, those from groups 2 and 5 for one week, and those from groups 3 and 6 for two months. All specimens were processed for scanning electron microscope (SEM) examination. Apatite precipitation on sealer and sealer–dentine interfaces was quantified using image analysis software (ImageJ). Energy dispersive X-ray (EDX) was used to analyse calcium (Ca) and phosphate (P) contents of surface precipitation on which calculation of the calcium phosphate (Ca/P) ratio was based.Results:TFBCS samples, regardless of the duration of SBF soaking, yielded a significantly higher surface area of precipitation compared to MTAFS (P<0.05); such precipitation increased over time, and the differences among the three time-points were also statistically significant. Following one day of SBF soaking, MTAFS samples showed only limited precipitation that started to appear after one week. EDX showed that Ca content and the Ca/P ratio of surface deposits on TFBCS samples increased over time with no difference between one week and two months of SBF soaking. The Ca content and Ca/P ratio of surface deposits on MTAFS were significantly lower than that of TFBCS samples regardless of the SBF soaking time.Conclusion:Ageing TotalFill BC sealer in SPF can induce considerable apatite formation. In addition, the TotalFill BC sealer surface showed high Ca2+ ion release as reflected by the formation of apatite with a high Ca/P ratio. These bioactivity features increased over time. In comparison, the MTAFS appears to have lower and delayed bioactivity.

Endodontic sealers and post-endodontic waiting time affect the bond strength of the fiber posts

Abstract Introduction The resistance adhesive of a fiber post can be affected by several factors, such as the endodontic sealer and post-endodontic waiting time. Objective The aim of this study was to evaluate the effect of different endodontic sealers and two different post-endodontic waiting times on the bond strength of fiber posts. Material and method Seventy-two bovine teeth were endodontically treated and filled using three endodontic sealers: eugenol-based, epoxy resin-based, or mineral trioxide aggregate-based. The specimens were stored at 37°C for 24 hours or for 30 months. After the respective storage times, the root canals were prepared for luting fiber posts using RelyX U200. Push-out tests and analysis of failures were performed. The push-out data were analyzed by two-way analysis of variance to compare the effects of the endodontic sealer and with the t-test to compare the effects of post-endodontic waiting time. Result The AH Plus sealer yielded the highest bond strength values at 30 months post-endodontics (11.26 Mpa) (p &lt; 0.05), however no had difference with Endofill sealer at the same time. Endofill and MTA Fillapex sealers did not differ significantly in their effects, irrespective of the post-endodontic waiting time. Conclusion In conclusion, the endodontic sealer used and post-endodontic waiting time affect the adhesive resistance of fiber posts. The adhesion increases significantly when the fiber post is cemented 30 months after the root canal filling, while the adhesion is reduced when cementing immediately after root canal treatment, in particular for eugenol-based endodontic sealers.

Evaluation of the amount of remained sealer in the dentinal tubules following re-treatment with and without solvent.

Background:The effect of solvent and sealer type (BioRoot RCS [BRRCS], mineral trioxide aggregate [MTA] Fillapex, and AH26) on the root canal residues was evaluated by confocal laser scanning microscopy (CLSM).Materials and Methods:Distal root canals of 60 mandibular molars prepared up to ProTaper-F4 were filled using one of the sealers labeled with 0.1% rhodamine-B (n = 20 per sealer). Samples were divided into two according to reprocessing methods as follows: ProTaper Universal Reprocessing System (PTUR) or solvent plus PTUR. Samples were sectioned at 2–5 and 8 mm from the apex and the samples were observed under CLSM. Penetration depth, percent penetration depth, and percent penetration into canal walls were evaluated. Statistical analysis was performed using the repeated measures of the two-way ANOVA and Bonferroni post hoc test at P = 0.05.Results:The highest penetration depth was measured in BRRCS in all root canal thirds with or without chloroform addition. Percentage of penetration depth values of MTA Fillapex and BRRCS was statistically similar and higher than AH26 in all sections, except the apical section re-treated with the addition of chloroform.Conclusion:None of the sealers were completely removed. The type of sealant and the use of chloroform changed the amount of sealant remaining in the root canal system.

Evaluation of physico-chemical properties, time of hardening and draining of an endodontic sealer added to mta in different proportions

Objetivo: o objetivo desta pesquisa foi avaliar o tempo e o fluxo de endurecimento do cimento AH-PLUS e MTA nas proporções de 10%, 20% e 30%, a fim de adicionar a capacidade de estimular a deposição de tecido duro. Material e Métodos: Para o teste de fluxo, 0,5 ml de cimento foram depositados em uma placa de vidro e outra placa foi adicionada com uma carga adicional, após 10 minutos a carga foi removida e o diâmetro do círculo formado pelo cimento foi medido com um Bússola digital. Para o tempo de endurecimento, foram utilizados moldes cilíndricos preenchidos com o cimento testado, mantidos dentro de um forno e os testes de agulha de Gillmore foram realizados no material em intervalos de 60 segundos até que as marcas não fossem visíveis na superfície do cimento testado. Resultados: Na vazão, os valores obtidos foram: 41,1 mm (10% MTA), 32,86 mm (20% MTA), 21,91 mm (30% MTA), 24,41 mm AHPlus® e 26,47 mm MTA Fillapex®. O tempo de endurecimento encontrado nesta pesquisa foi de 1631 'em proporção (10% MTA), 628,4 em proporção (20% MTA), 567' em proporção (30% MTA), 767 em AH Plus e Fillapex MTA não. presou dentro de 6 semanas. Conclusão: concluiu-se que a adição de 10%, 20% e 30% de MTA ao AH Plus não alterou as propriedades do fluxo e o tempo estabelecido.

Evaluation of the radiopacity of selected calcium-silicate root canal sealers

Abstract Introduction: International standards require adequate radi-opacity of root canal filling materials to distinguish them from natural structures, such as tooth tissues, and to allow evaluation of the quality of the root canal filling. The aim of this in vitro study was to assess the radiopacity of selected calcium-silicate root canal sealers compared with the resin-based AH Plus root canal sealer. Materials and methods: The study included 6 root canal sealers: BioRoot RCS, GuttaFlow bioseal, MTA Fillapex, Total Fill BC Sealer, Well-Root ST and AH Plus. Six disc-shaped samples were prepared from all analysed materials (4 mm in diameter and 1 mm thick) and analysed using a digital radiography technique and aluminium step wedges (1.5–15 mm thick) as a standard reference. The radiopacity was determined using computer image analysis. The data was statistically analysed using a Tukey’s range test. Results: All root canal sealers showed radiopacity at least corresponding to the 3 mm aluminium step wedge. AH Plus (13.23 mm Al) showed the highest radiopacity, followed by Total Fill BC Sealer (7.93 mm Al), Well-Root ST (7.12 mm Al), BioRoot RCS (5.93 mm Al) and GuttaFlow bioseal (5.08 mm Al). MTA Fillapex (3.02 mm Al) showed the lowest radiopacity. Conclusions: Although the evaluated root canal sealers show different radiopacities, all the materials met the criteria laid down by the International Organization of Standardization.