Mimosa Pigra Research Articles

Effect of Mimosa pigra conjugated zinc nanoparticles on seed germination and seedlings growth of Solanum lycopersicum

Neutralizing Effects of Mimosa pigra Stem-Bark against Selected Snake Venom from Malaysian Habitat

Giant false sensitive plants (Mimosa invisa Colla) and giant sensitive plants (Mimosa pigra L.) are invasive plant species in the tropics and native to America. They are widely distributed in different ecosystems and soil types. Apart from being considered woody shrub weeds, these plant species can be used as herbal medicine for their antioxidant activities. An experiment to study the antioxidant activities and phytochemicals of M. invisa and M. pigra grown at 200 m from the coastline and close to the riverbank (riparian abandoned land) was carried out from March to November 2023. Weed leaves were collected as purposive randomized sampling from different terrestrial habitats in the City of Padang, West Sumatra. Antioxidant activity was identified according to a 2-2-diphenyl-1-picrilhydrazil (DPPH) radical-scavenging assay, and phytochemical compounds were identified qualitatively. Results demonstrate that M. invisa had mild antioxidant activity and M. pigra had strong antioxidant activity. The IC50 values for M. invisa grown at 200 m from the coastline and at the riverbank were 121.3 ± 11.5 and 105.6 ± 2.90 ppm, respectively. The IC50 values of M. pigra grown at 200 m from the coastline and at the riverbank were 80.6 ± 15.9 and 85.1 ± 9.03 ppm, respectively. Phenolic, saponin, and steroid were detected in all weeds grown at different habitats. However, alkaloids and terpenoids were not detected. Interestingly, flavonoid was not detected in the leaves of M. pigra grown at the riverbank.

Antioxidants are essential for neutralizing free radical and their presence in plants like mimosa weeds could be beneficial for various applications, include medical uses and agricultural development. This research focus on the potential antioxidant content of mimosa weed species (Mimosa pudica L., Mimosa invisa L., Mimosa pigra L.) from various plant parts (roots, stems, leaves and seed) using the DPPH method (2,2-diphenyl-1-picrylhydrazyl). This study aims to understand the interaction between different types of mimosa weeds and various plant parts in relation to antioxidant activity levels, as well as phytochemical content across the three types of Mimosa sp. using the DPPH method and determine the best-performing weed and plant parts based on the smallest IC50 value and highest phytochemical content. This research was conducted in Padang City. Sample drying and extraction were carried out at the Plant Physiology Laboratory, Faculty of Agriculture, Andalas University, and phytochemical tests were performed at Vahana Scientific Laboratory Padang. The research took place from February to May 2024. The design used was a factorial Completely Randomize Design (CRD). The first factor is the treatment of Mimosa sp. weed species, namely: Mimosa pudica L., Mimosa invisa L. and Mimosa pigra L. The second factor was the treatment of different parts of the Mimosa sp. Namely: root, stem, leaf and seed. From these two factors, 12 treatment combinations were obtained, each repeated three times, resulting in a total of 36 experimental units. The research data were analyzed statistically with the F test at the 5% level and if F count > F table at 5%, then it continued with Duncan’s New Multiple Range Test (DNMRT) at the 5% level. The results showed that there was an interaction between mimosa weed species and plant parts in terms of antioxidant activity, as indicated by the IC50 values. The strongest antioxidant was found in the leaves of Mimosa pigra L., with an antioxidant value of 41.89 mg/L.

This study explores the impact of seed nano-priming with green-synthesized copper oxide nanoparticles (CuO NPs) using Mimosa pigra leaf extract on the germination and seedling growth of tomato (Solanum lycopersicum). CuO NPs were synthesized through a plant-mediated green synthesis approach, where phytochemicals in M. pigra extract reduced Cu²⁺ ions and stabilized the nanoparticles. The resulting nanoparticles were characterized using UV-vis spectroscopy and SEM, revealing a surface plasmon resonance (SPR) peak at 224 nm and a nanoscale morphology with an average size of 108 nm. XRD analysis confirmed a crystalline monoclinic structure, with an average crystallite size of 30.68 nm. FTIR spectra showed characteristic Cu-O bond vibrations and plant-related functional groups, confirming successful nanoparticle synthesis. Seed germination experiments evaluated the effects of CuO NPs across a concentration gradient (0–1000 ppm). Results demonstrated a biphasic effect on germination and seedling growth. Low concentrations (5–100 ppm) enhanced germination percentages and growth metrics, while higher concentrations (≥500 ppm) inhibited these parameters. The 50 ppm treatment exhibited the highest germination rate, whereas 1000 ppm significantly suppressed seed germination and seedling growth. Statistical analysis indicated significant differences in root and shoot lengths across treatments, with oxidative stress and genotoxicity attributed to higher CuO NP concentrations as key inhibitory factors.These findings highlight the dual role of CuO NPs, emphasizing the potential of green-synthesized nanoparticles as bio-enhancers at optimal concentrations, while cautioning against their phytotoxic effects at elevated levels. This research underscores the need for further studies to optimize nanoparticle applications in agriculture and mitigate environmental risks.

Susupan gunung (Mimosa pigra L.) is a weed that grows wild and is often found in peatlands. This plant can grow in tropical and subtropical regions. Phytochemical analysis of the leaves of susupan gunung has shown that the leaves contain tannin compounds, indicating the potential of these leaves to be a source of natural textile dyes. Natural dyes can replace synthetic dyes in the textile industry, which can pollute the environment due to their carcinogenic nature. This research to find the optimal operating conditions for extracting natural dyes from susupan gunung leaves and to test the dyeing quality of cotton fabric using susupan gunung leaf extract. The variables used in the extraction process are temperature, time, and ratio of raw material to solvent volume. The tannin concentration in the extract was analyzed using the volumetric method and optimized using Response Surface Methodology (RSM) with the Box-Behnken model. Fabric dyeing was performed using alum, iron, and calcium as mordants after dyeing the fabric. The result of this research indicate that the optimal operating conditions are at a temperature of 77oC, a time of 40 minutes, and a raw material to solvent volume ratio of 1:8, with a tannin concentration of 0,004 g/ml. The best fabric dyeing quality results were obtained using alum mordant, with a colorfastness rating of 4-5 (good) for washing and a colorfastness rating of 5 (very good)for rubbing.

Wetlands in East Africa are important for providing ecosystem services and for conserving biodiversity. They are also suitable and increasingly used for agriculture. Between cropping cycles, spontaneous vegetation regrows on fallow plots. We hypothesize that recovery is affected by hydro-edaphic conditions and the duration of the fallow period. Land use intensification reduces fallow durations. A multi-year field study investigated the dynamics, biodiversity, and potential uses of fallow vegetation species after a disturbance event in an inland valley wetland in Central Uganda. The wetland was stratified into three hydrological positions along a gradient comprising the valley fringe, the mid-valley, and the riparian zone. In each zone, biomass was removed, and the soil was tilled, simulating a common disturbance event. Subsequently, four plots of 4 × 4 m size were delineated in each zone. Vegetation regrowth was subsequently monitored over a period of two years. We recorded and analyzed changes in aboveground live biomass, abundance of selected plant species, taxonomic and functional composition, and evenness during a 27-months recovery phase. While annual species dominated the vegetation initially, these were gradually replaced by herbaceous perennials, and eventually by tall reeds and woody plants, constituting three successional stages. The dynamics were similar, but species composition differed across the positions. At all successional stages, we observed the presence of useful wild plants, but also invasive species such as Mimosa pigra were recorded. While temporary fallows are important for biodiversity in tropical wetlands, such successions cannot substitute for the functions and services provided by natural vegetation and may promote invasive species.

Background and objectives: Various parts of Mimosa pigra (MPG) are used in traditional medicines to treat convulsive disorders. The objective of this study was to investigate the anticonvulsant properties of Mimosa pigra ethanol root extract (EREM).Methods: The acute toxicity of the extract was investigated using OECD 423 protocol of 2002. The anticonvulsant properties of EREM at 200,400 and 800 mg/kg were evaluated using Maximal Electroshock Test (MEST) in chicks; strychnine (SCN-) and pentylenetetrazole (PTZ)-induced seizures in mice.Results: The extract at 400 and 800 mg/kg significantly (p<0.05) prolonged the mean onset of clonic and tonic convulsions in mouse model of SCN-induced seizure. In PTZ-induced seizure, the extract at 400 mg/kg significantly (p<0.05) increased the mean onset of clonic seizure, while at 800 mg/kg, there was significant (p<0.05) prolongation in the mean onset of clonic and tonic seizure compared to control. The extract did not protect the chick against MEST but significantly (p < 0.05) reduced the mean recovery time at the of 200, 400 and 800 mg/kg. The extract offered 60 and 100% protection at 400 and 800 mg/kg respectively in SCN-induced seizure. Similarly, EREM offered 20 and 40% protection at 400 and 800 mg/kg respectively in PTZ-induced seizure. Diazepam (10 mg/kg), a reference drug significantly (p<0.05) prolonged the onset of clonic-tonic seizure and protected against SCN-, and PTZ-induced convulsion in mice.Conclusion: These findings indicated that EREM may possess anticonvulsant activity in SCN-, and PTZ-induced seizure in mice. Thus, lend scientific credence to the anticonvulsant claim of EREM in ethnomedicine.

The Rapid Assessment of Invasive Alien Species in Wetlands of Lao PDR, was conducted to assess the status of four key invasive alien species such as Giant mimosa (Mimosa pigra), Water hyacinth (Eichhornia crassipes), Golden apple snail (Pomacea canaliculata), Suckermouth catfish (Hypostomus plecostomus) at three wetland sites, including Beung Kiat Ngong in Champasack Province, Xe Champhone in Savannakhet Province and Nong Nga Swamp in Borlikhamxay Province. The study included field surveys and interviews of key informants from communities. The study showed that all invasive species are commonly found in wetlands, streams, riverbanks, ponds and rice fields. Local people opinion on floods brought and distributed IAS over these areas. Only Suckermounth catfish is not significant in all three study sites. Mimosa pigra is the most concerned from people’s opinion. It is fast growing and difficult to manage, having no benefits to human and environment. Water hyacinth impacts on water flow, creating thicker sludges along wetland edges, riverbanks and oxbow swamps. Water hyacinth is of highest concerns in Xe Champhone wetlands. People are having difficulties in fishing and boat travelling where the giant mimosa and water hyacinth are invading. When Golden apple snail is present, the variety of aquatic resources is getting loss, such as smalls fishes, shrimps, native snails. Golden apple snails also ate rice plants. However, some families, collect apple snails for sell as an extra income. No special management plan from local government or development projects for invasive species eradication. Some attempts can be done by communities to manage IAS such as cutting, ploughing, and burning mimosa. People collected water hyacinth by hand, left on the ground to dry and burn. For apple snails, farmers collected from their own fields let them dry on the ground. A collaboration on IAS eradication environmentally is required, financial supports from government and international organization is needed.

Invasive foreign species (JAI) are the most serious problem for ecosystems and biodiversity, especially in protected areas. Its fast and aggressive growth makes it easy for it to dominate the land. Tight competition for water, light and nutrients is a serious problem. Therefore, the aim of the research carried out along the Pertamina Complex Patra Asri road, Bandung City, is to determine the diversity of invasive foreign plant species found along the Pertamina road, Patra Asri Complex. The research method was carried out by collecting data directly. Invasive foreign plant species found in the category of spreading to other trees are Mikania micrantha and Ipomoea Cairica. The other types are Mimosa pigra, Imperata cylindrica, Bidens Pilosa L., Paspalum distichum L., and Sesbania drummondii. The impact of invasive plants spreading on other plants will reduce and threaten local growth. Invasive plants can also harm local plants in terms of physiology and morphology as they try to defend and compete for nutrients with invasive plants. Environmental damage due to invasive plants will result in physical damage to the habitat, such as blocking waterways, damaging building structures and disturbing beauty.

The diversity in structure and herbicidal properties detected in natural phytotoxic compounds could bring about advantages for development bio-herbicides. The present study was carried out search for potential weed inhibitors from the parts of Mimosa pigra L. The ethyl acetate (EtOAc) extract of leaf of M. pigra showed inhibitory activity during the time that Echinochloa crus-galli (barnyardgrass) germinates and grows, which is greater than that of other extracts. From this active extract, potent growth inhibitors were isolated and identified by column chromatography (CC), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (1H and 13C NMR). The six compounds were purified in this study namely: lupeol (C1, 13.2 mg), stigmastane-3,6-dione (C2, 14.7 mg), quercetin (C3, 20.2 mg), chrysoeriol (C4, 28 mg), methyl gallate (C5, 21.5 mg) and daucosterol (C6, 16.0 mg). The C2 (quercetin) compound completely inhibited the emergency, shoot height and root length of E. crus-galli at 1 mg/mL concentration (IC50 shoot height = 0.56 mg/mL). This was also the first study to report the isolation and allelopathic activity of lupeol, chrysoeriol and daucosterol from M. pigra leaf. Findings of this study highlighted that quercetin from M. pigra may become bio-herbicide to control barnyard grass and other grass weeds for the development of safe agriculture.

Kruger National Park (KNP), South Africa, covers an area of 20,000 km2 and is one of the world’s most widely known protected areas. Like many protected areas, KNP is facing an increasing problem with the introduction and spread of alien plants. However, species prioritization using poor baseline data remains a key challenge for managers globally. Publications, expert opinion, and field observations indicate that 407 alien plant taxa have been recorded in the KNP; this list also included hybrids, ornamentals, records that could only be identified to the genus level, and extralimitals (i.e., species native elsewhere in South Africa; 22 species in total); in addition, two species are considered eradicated, and three potentially eradicated. Such extensive lists of poorly defined species’ statuses accumulated over long periods of time poses challenges to current decision-making processes. This is especially important for the management of naturalized (maintaining self-sustaining populations) and invasive species (subset of naturalized species that have spreading populations), because management needs to identify and target high-priority species and vulnerable sites. Here, we provide an up-to-date inventory of alien plant species that occur in natural areas in KNP (i.e., beyond tourist camps and other infrastructure) and thus represent a potential threat to the native species diversity in the park. We identified 146 such alien taxa, of which 30 are casuals, 58 are naturalized, 21 have become invasive, and for 37 species, the status remains to be determined. Twelve of the invasive species in KNP are globally widespread, occurring in more than 100 regions, and five (i.e., Pontederia crassipes, Lantana camara, Opuntia stricta, Chromolaena odorata and Mimosa pigra) are listed among 100 of the world’s worst invasive alien species. The alien flora in KNP comprises 41 families. Solanaceae (45.5%) and Asteraceae (26.1%) are over-represented among invasive species compared to non-invasive species. The alien flora of KNP mostly originates from North America and South America, and largely consists of perennials and herbaceous species. We found no significant results regarding the effect of origin and life span on invasion status. Despite a steady increase in the numbers of alien and naturalized plants since the 1980s, species we classified as invasive generally represent earlier introductions and have not increased substantially since the beginning of this century. Our paper highlights that carefully revised naturalized and invasive species lists reflecting the current situation in protected areas present a strong knowledge base for effective management strategies. In addition to addressing data gaps related to the distribution of alien species on a global scale, knowledge from large protected areas such as KNP contributes to understanding invasions in landscapes with varying and unique habitat types.

Enhanced adsorptive removal of hexavalent chromium in aqueous media using chitosan-modified biochar: Synthesis, sorption mechanism, and reusability

Anti-diabetic activity of Mimosa pigra Linn (Fabaceae) methanol leaf extract on alloxan-induced diabetic rats

The leaves of Mimosa pigra have been reported in ethnobotany to be used for the management of the symptoms of diabetes. This study is to investigate the anti-diabetic activity of the methanol extract of the leaves of this plant. The leaves were dried, pulverized and macerated successively in n-hexane, acetone and methanol. The methanol extract (1 g/kg body weight) was evaluated in vivo for anti-diabetic activity using adult male Wistar albino rats. Alloxan (150 mg/kg b.wt.) was used for induction of hyperglycemia, and Metformin (2.5 mg/kg) was used as the standard oral hypoglycemic agent. Blood glucose levels were monitored with a glucometer. The data is presented as Standard deviation (±) of the mean of blood glucose levels (mg/dl), and analyzed using one way analysis of variance (ANOVA) at (p<0.05). The methanol extract of the leaves showed significant percent decrease (p<0.05) in blood glucose levels of 3.80, 7.15 and 10.35 %, at 60, 120 and 180 min, respectively, on Day 1. And from Day 2 to Day 7 there was a continued decrease of 10.75, 16.36, 22.09, 29.06, 33.4 and 36.67 %, respectively, compared to the control. For the same period, the standard drug Metformin demonstrated greater decreases of 24.45, 30.38, 37.37, 40.93, 46.18 and 52.30 %, respectively. Phytochemical screening revealed the presence of flavonoids and tannins in the methanol and acetone extracts, while saponins and triterpenes were present in both the hexane and methanol extracts. The presence of these metabolites could be responsible for the observed anti-diabetic activity of this plant.

Ipomoea aquatica (Water Spinach) is the most potential for livestock growth performance, including chickens, pigs, cattle, and goats, especially in a tropical country like Cambodia. It is not only an alternative feed source but also one kind of supplemented feed for goat raising. Supplementation with Water Spinach in the utilization of low-quality tree foliage results in an increase in dry matter intake in goat production. This study aimed to identify the effectiveness of supplementation of Water Spinach in the utilization of Mimosa pigra and Leucaena leucocephala leaf in in vitro fermentation. The study was designed according to a 2 × 2 factorial arrangement in randomized design of seven treatments with different ratios consisted of different three types of dietary treatments, including M. pigra, L. leucocephala, and Water Spinach. The treatments were arranged according to a completely randomized design and were as follow: T1 = M. pigra leaf (100%); T2 = L. leucocephala leaf (100%); T3 = M. pigra leaf and L. leucocephala leaf (50% and 50%); T4 = M. pigra leaf and Water Spinach (99.5% and 0.5%); T5 = L. leucocephala leaf and Water Spinach (99.5% and 0.5%); T6 = M. pigra leaf and Water Spinach (99% and 1%); and T7 = L. leucocephala leaf and Water Spinach (99% and 1%). A total of 200 mg (dry matter) of dietary treatments were prepared in a 60 mL syringe. Each treatment was replicated 3 time. Gas recording of each treatment lasted for 3 days. In vitro was performed for 72 h, was followed by Makkar method. Gas production was recorded at 2, 4, 8, 12, 24, 36, 48, and 72 h of incubation by using strict anaerobic technique. A mixture of rumen fluid and dietary treatments were carried out under continuous flushing with CO2 in sharking incubator at 39°C. After incubating for 72 h, the ammonia concentration (NH3-N) was measured and recorded to identify pH, nutrient digestibility, and ammonia concentration (NH3-N). Nutrient digestibility of the treatment with Water Spinach supplement in the utilization of L. leucocephala was obtained at a higher digestibility than treatment with M. pigra (p < 0.05). Gas production was different between groups (p < 0.05). Treatment with only M. pigra leaf had the highest gas production (A), while treatment with Water Spinach supplementation had the lowest gas production (A). At 0-24 h, the treatment with L. leucocephala leaf and Water Spinach 0.5% had the highest gas production, but after 24 h, M. pigra leaf and Water Spinach 1% and L. leucocephala leaf and Water Spinach 0.5% produced more gas compared to the other treatments (p < 0.05). The supplementation of Water Spinach 1% in treatment with M. pigra and L. leucocephala leaf resulted in increased degradability, gas production, and NH3-N concentration without a change in the pH value rumen condition. Based on these results, it is recommended that the level of Water Spinach supplementation should be 1% of dietary intake. Future studies should consider investigating the rumen ecology associated with Water Spinach supplementation. Feeding with Water Spinach remains a good supplement for ruminant performance; therefore, further studies should be conducted using Water Spinach in ruminant feeding in both metabolic and feeding trials.

Effects of replacing soybean meal with Giant Mimosa (Mimosa pigra L.) leaf meal in diet on productive performance, carcass characteristics, and apparent digestibility of native chickens (Leung Hang Kao)

The expansion of cattle ranching in the coastal plain of the Gulf of Mexico is transforming freshwater swamps resulting in the reduction of swamps area and the introduction of invasive exotic grasses. This study seeks to determine the structure of the remaining population of Annona glabra in a grassland, evaluate vegetation and environmental conditions of the grassland, and determine the germination capability of A. glabra under different cover conditions of grass growth and two flood levels. The study area is a flooded grassland invaded by Echinochloa pyramidalis in central Veracruz, Gulf of Mexico. In the grassland, we censused and mapped the remaining individuals of A. glabra. We sampled the vegetation and measured the physicochemical characteristics of the soil and water. To evaluate the effect of the invasive grass on the germination of A. glabra seeds, we designed two greenhouse experiments: one (E1) under soil moisture at field capacity and another (E2) under flooded conditions. We found 312 remnant individuals. We recorded 31 plant species, most (72.7%) classified as aquatic/semiaquatic. The relative importance value was highest for E. pyramidalis, Mimosa pigra, and Eleocharis mutata. BEST analysis did not detect significant correlations between vegetation and physicochemical variables, indicating homogeneous environmental conditions. Seed germination was above 80% in E1 and below 20% in E2 in all grass cover levels. Although flooding decreases germination rates, substantial proportion of seeds can germinate, suggesting that natural succession is viable. Based on our results, the flooded grassland has conditions to restore swamp.

Populations of invasive alien plants create disruptive plant communities that are extremely adaptable, imposing severe ecological impacts on agriculture, biodiversity and human activities. To minimise these impacts, prevention and effective weed management strategies are urgently required, including the identification of satellite populations before they invade new areas. This is a critical element that allows weed management practices to become both successful and cost-effective. Mimosa pigra L. (Giant sensitive plant) is an invasive weed that has spread across various environments around the world and is considered one of the world’s top 100 most invasive plant species. Being adaptable to a wide range of soil types, in addition to its woody protective prickles and low palatability, M. pigra has quickly spread and established itself in a range of habitats. Current control methods of this species include biological, chemical and physical methods, together with attempts of integrated application. Reports suggest that integrated management appears to be the most effective means of controlling M. pigra since the use of any single method has not yet proved suitable. In this regard, this review synthesises and explores the available global literature and current research gaps relating to the biology, distribution, impacts and management of M. pigra. The contribution of this work will help guide land managers to design appropriate and sustainable management programs to control M. pigra.

Antecedentes y Objetivos: Actualmente existe deterioro y disminución de selvas inundables debido a la deforestación para ganadería e introducción de pastos exóticos. Los objetivos del presente estudio fueron evaluar el porcentaje de supervivencia de plántulas de Annona glabra, el cambio en la vegetación acompañante, y estimar los costos de los tratamientos experimentales de restauración ecológica de una selva inundable de A. glabra transformada en pastizal e invadida por el pasto exótico Echinochloa pyramidalis. Métodos: El estudio se realizó en un pastizal inundable en la costa central de Veracruz, México. Consistió en sembrar cuatro plántulas de A. glabra en cuadros experimentales expuestos a distintas técnicas de restauración. Se probaron 15 tratamientos experimentales que consistieron en cinco técnicas de restauración (sin modificación, cubierta plástica, plantación de Pontederia sagittata, remoción de suelo, y elevación del suelo) y tres pretratamientos aplicados a las plántulas sembradas (plántulas de vivero con y sin fertilizante, plántulas de selva) en un diseño de bloques al azar.Resultados clave: Se registró baja supervivencia de plántulas (30.7%), siendo mayor en plántulas de selva (41.1%). El mayor porcentaje de supervivencia se registró con la técnica de elevación del suelo, seguido de la de remoción. Se registraron 40 especies en la vegetación; la mayor riqueza específica se observó en las técnicas de elevación del nivel del suelo y remoción. Las especies con mayor Valor de Importancia Relativa fueron Echinochloa pyramidalis, Mimosa pigra y Annona glabra. El pretratamiento en el cual se obtuvieron las plántulas directamente de la selva fue el más económico.Conclusiones: Se requieren acciones para incrementar el éxito de la reforestación. Para futuros proyectos de restauración, recomendamos la elevación del suelo en áreas de transplante y cubierta de plástico previo a la plantación, para incrementar la probabilidad de supervivencia, y reducir la cobertura del pasto; además de la reforestación con plántulas colectadas de un fragmento de selva.