Mild Thermal Treatment Research Articles

Exploring the Power of Thermosonication: A Comprehensive Review of Its Applications and Impact in the Food Industry

Thermosonication (TS) has been identified as a smart remedy for the shortcomings of heat treatment, which typically requires prolonged exposure to high temperatures. This technique combines moderate heat treatment with acoustic energy to eliminate harmful microorganisms and enzymes in food products. Unlike conventional heat treatment, thermosonication utilizes short holding times, allowing for the preservation of food products' phytochemical compounds and sensory characteristics. The benefits and challenges of this emerging technology, such as equipment cost, limited availability of data, inconsistent results, high energy consumption, and scale-up challenges, have been assessed, and the design process for using ultrasound in combination with mild thermal treatment has been discussed. TS has proven to be a promising technique for eliminating microorganisms and enzymes without compromising the nutritional or sensory quality of food products. Utilizing natural antimicrobial agents such as ascorbic acid, Nisin, and ε-polylysine (ε-PL) in combination with thermosonication is a promising approach to enhancing the safety and shelf life of food products. Further research is required to enhance the utilization of natural antimicrobial agents and to acquire a more comprehensive comprehension of their impact on the safety and quality of food products.

Viability and removal assessment of Escherichia coli and Salmonella spp. by real-time PCR with propidium monoazide in the hygienization of sewage sludge using three anaerobic processes

Sewage sludge should be stabilized for its beneficial use and pathogens, among other factors, should comply with environmental regulations. Three sludge stabilization process were compared to assess their suitability for producing Class A biosolids: MAD-AT (mesophilic (37 °C) anaerobic digestion (MAD) followed by an alkaline treatment (AT)); TAD (thermophilic (55 °C) anaerobic digester); and TP-TAD (mild thermal (80 °C, 1 h) pretreatment (TP) followed by a TAD). E. coli and Salmonella spp. were determined, differentiating three possible states: total cells (qPCR), viable cells using the propidium monoazide method (PMA-qPCR), and culturable cells (MPN).Culture techniques followed by the confirmative biochemical tests identified the presence of Salmonella spp. in PS and MAD samples, while the molecular methods (qPCR and PMA-qPCR) showed negative results in all samples. The TP + TAD arrangement reduced the concentration of total and viable E. coli cells in a greater extent than the TAD process. However, an increase of culturable E. coli was observed in the corresponding TAD step, indicating that the mild thermal pretreatment induced the viable but non-culturable state in E. coli. In addition, the PMA technique did not discriminate viable from non-viable bacteria in complex matrices. The three processes produced Class A biosolids (fecal coliforms < 1000 MPN/gTS and Salmonella spp, < 3 MPN/gTS) maintaining compliance after a 72 h storage period. It appears that the TP step favors the viable but not culturable state in E. coli cells, a finding that should be considered when adopting mild thermal treatment in sludge stabilization process arrangements.

Spatially resolved micron-scale wrinkle structures at asphaltene films induced by mild thermal treatment and its impact on emulsion stability

Mild thermal treatment is an important partial upgrading technique to enable bitumen pipeline transportation, but no attention has been paid to the impact of mild thermal treatment on the emulsification behavior of emerging partially upgraded bitumen. Asphaltene compounds are active emulsion stabilizers in bitumen oil. The emulsion stabilizing capacity of bitumen asphaltenes was investigated, before and after a mild thermal treatment at 400 °C. The structural morphology and mechanical property of the asphaltene interfacial films were analyzed by using a combination of cryo-SEM, Langmuir trough, and Brewster angle microscopy. The thermal treatment significantly enhanced the emulsion stabilizing capacity of bitumen asphaltenes; the interfacial films formed by the thermally treated asphaltene samples appeared to be rougher and thicker with more abundant micron-scale wrinkle structures. The interfacial corrugation may intensify the mechanical stability/flexibility of the asphaltene films and consequently strengthen the stability of emulsion droplet.

An Economical and Scalable Method to Synthesize Graphitic-Like Films.

An economical and facile method to synthesize a precursor for carbon films and materials has been developed. This precursor can be easily coated onto substrates without binder reagents and then converted into a graphitic-like structure after mild thermal treatment. This approach potentially allows the coating of glass surfaces of different shapes and forms, such as the inside of a glass tube, for instance. The precursor consists of tetrahedral halocarbyne units which randomly combine through single electron transfer with organometallic compounds to create a poly(carbyne)-like polymeric material. Advanced characterization tools reveal that the synthesized product (poly(halocarbyne) or PXC, where X indicate the presence of halogens, is composed mostly of carbon, hydrogen, and a variable percentage of residual halocarbon groups. Therefore, it possesses good solubility in organic solvents and can be coated on any complex substrate. The coated PXC material produced here was annealed under mild conditions, leading to the production of a graphitic-like film on a glass substrate. The chemical homogeneity of the carbon material of the film was confirmed by Raman spectroscopy.

Functional Properties of an Oat-Based Postbiotic Aimed at a Potential Cosmetic Formulation

The concept of postbiotic has been attracting the attention of the scientific community and several industrial realities to develop new claims and new market segments for functional fermented products. The aim of this work was to develop a process to produce an oat-based postbiotic ingredient to be used in personal care cosmetic formulations. A hydrolyzed oatmeal suspension was fermented using Lacticaseibacillus paracasei CBA L74 as starter culture, at 37 °C for 48 h by controlling the pH; then the bacterial charge was inactivated by a mild thermal treatment at 80 °C for 30 s, obtaining a postbiotic. The effect of different process steps, hydrolysis, sterilization, fermentation, and inactivation phases, on lactic acid concentration, total polyphenolic content, antioxidant activity, tyrosinase inhibition activity and Sun Protection Factor value was investigated, demonstrating the potential cosmetic applications. The maximum bacterial growth and lactic acid production were achieved after 24 h of process, with a cell density and a lactic acid concentration of 3.05 × 109 CFU/mL and 8.60 g/L, respectively. The total phenolic content and the antioxidant activity reached their maximum values (2.5 mgGAE/mL, EC50 = 2.2 mg/mL and 1.38 × 10−2 mmol Fe2+/g and 7.3 × 10−3 mmol TE/g, respectively) after the sterilization treatment; the maximum tyrosinase inhibition of 50.6%, corresponding to a sample concentration of 16 mg/mL, was found after 24 h of fermentation process. Fermentation did not show an impact on UV shielding ability and the SPF value decreased during the process.

Effect of Pasteurization by Moderate Intensity Pulsed Electric Fields (PEF) Treatment Compared to Thermal Treatment on Quality Attributes of Fresh Orange Juice.

Novel pulsed electric field (PEF) process conditions at moderate electric field strength and long pulse duration have recently been established to obtain microbial inactivation. In this study, the effect of these PEF conditions (E = 0.9 and 2.7 kV/cm, with pulse duration 1000 µs) at variable maximum temperatures was evaluated on quality attributes of freshly squeezed orange juice. Results were compared to orange juice that received no treatment or a mild or severe thermal pasteurization treatment. No differences for pH and soluble solids were found after application of any treatment, and only small differences were observed for color and vitamin C content (ascorbic acid and dehydroascorbic acid) after processing, mainly for conditions applied at higher temperature. Variations in the maximum temperatures of the PEF and thermal processes led to differences in flavor compounds and the remaining activity of pectinmethylesterase (PME). At PEF conditions with a maximum temperature of 78 °C or higher, PME activity levels were below a critical value, meaning that the cloud is stable. At this temperature volatiles associated with fresh juice (such as octanal and nonanal) are statistically identical to untreated juice, while they are statistically distinguishable from thermal treated. This papers demonstrates the potential of using moderate intensity PEF as an adequate alternative to thermal pasteurization of orange juice with a better retention of the fresh flavor.

Material and Processing Considerations for Thick All Active Material Mildly Sintered Lithium-Ion Electrodes

Electrodes containing only electroactive material processed to relatively large thicknesses (often greater than 500 microns) can have high energy density at the cell level due to the high loading of electroactive material. The specific all electroactive lithium-ion material electrodes in this work undergo a mild thermal treatment and are referred to as sintered electrodes. While in general the great thickness results in long ion diffusion paths which limits rate capability of sintered electrode cells, the lack of inactive additives provides a relative advantage in effective ionic transport properties.In this talk, recent improvements in sintered electrode batteries will be discussed. In particular, incorporation of new materials with increased gravimetric and more importantly volumetric energy density will be described. In some cases, the alternative materials have lower electronic conductivity than previously used electroactive materials for sintered electrodes, and processing strategies for alleviating electronic conductivity limitations will be discussed. Incorporation of modified materials and processing results in very high energy density small form factor battery cells.

P-type conversion of distorted SnOx thin film by mild thermal annealing treatment in pure N2 environment

Tin oxide semiconductors can achieve both n- and p-type conduction, depending on the oxidation state of Sn. An n-type conduction can easily be fabricated; however, considerable optimization is required for fabrication of a p-type behavior. In this study, n-type SnOx thin films, prepared by reactive magnetron sputtering, were converted to p-type behavior using only post-deposition annealing at 600 °C in a pure nitrogen atmosphere. The annealing-temperature-dependent electrical properties of the SnOx thin films led to a remarkable increase in the yield of p-type behavior at 600 °C. X-ray diffraction analysis revealed that the SnOx film had a SnO2-dominant crystal phase and also suggested that N2 molecules dissociated at 600 °C and filled the oxygen vacancy (VO) site as atomic nitrogen. A detailed analysis of the binding state by x-ray photoelectron spectroscopy confirmed an increase in SnO-derived components, the appearance of peaks derived from N–Sn bonding, a decrease in VO caused by nitrogen doping, and charge transfer. Thus, we found that addition of nitrogen atoms promotes a chemical shift from Sn4+ to Sn2+ and that simultaneously passivates VO and contributes to hole generation.

Modifications of Thermal-Induced Northern Pike (Esox lucius) Liver Ferritin on Structural and Self-Assembly Properties.

Ferritin, as an iron storage protein, regulates iron metabolism and delivers bioactive substances. It has been regarded as a safe, new type of natural iron supplement, with high bioavailability. In this paper, we extracted and purified ferritin from northern pike liver (NPLF). The aggregation stabilities, assemble properties, and structural changes in NPLF were investigated using electrophoresis, dynamic light scattering (DLS), circular dichroism (CD), UV–Visible absorption spectroscopy, fluorescence spectroscopy, and transmission electron microscopy (TEM) under various thermal treatments. The solubility, iron concentration, and monodispersity of NPLF all decreased as the temperature increased, and macromolecular aggregates developed. At 60 °C and 70 °C, the α-helix content of ferritin was greater. The content of α-helix were reduced to 8.10% and 1.90% at 90 °C and 100 °C, respectively, indicating the protein structure became loose and lost its self-assembly ability. Furthermore, when treated below 80 °C, NPLF maintained a complete cage-like shape, according to the microstructure. Partially unfolded structures reassembled into tiny aggregates at 80 °C. These findings suggest that mild thermal treatment (80 °C) might inhibit ferritin aggregation while leaving its self-assembly capacity unaffected. Thus, this study provides a theoretical basis for the processing and use of NPLF.

Activating Molybdenum Carbide Nanoparticle Catalysts under Mild Conditions Using Thermally Labile Ligands.

Transition-metal carbides are promising low-cost materials for various catalytic transformations due to their multifunctionality and noble-metal-like behavior. Nanostructuring transition-metal carbides offers advantages resulting from the large surface-area-to-volume ratios inherent in colloidal nanoparticle catalysts; however, a barrier for their utilization is removal of the long-chain aliphatic ligands on their surface to access active sites. Annealing procedures to remove these ligands require temperatures greater than the catalyst synthesis and catalytic reaction temperatures and may further result in coking or particle sintering that can reduce catalytic performance. One way to circumvent this problem is by replacing the long-chain aliphatic ligands with smaller ligands that can be easily removed through low-temperature thermolytic decomposition. Here, we present the exchange of native oleylamine ligands on colloidal α-MoC1–x nanoparticles for thermally labile tert-butylamine ligands. Analyses of the ligand exchange reaction by solution 1H NMR spectroscopy, FT-IR spectroscopy, and thermogravimetric analysis–mass spectrometry (TGA-MS) confirm the displacement of 60% of the native oleylamine ligands for the thermally labile tert-butylamine, which can be removed with a mild activation step at 250 °C. Catalytic site densities were determined by carbon monoxide (CO) chemisorption, demonstrating that the mild thermal treatment at 250 °C activates ca. 25% of the total binding sites, while the native oleylamine-terminated MoC1–x nanoparticles showed no available surface binding sites after this low-temperature treatment. The mild pretreatment at 250 °C also shows distinctly different initial activities and postinduction period selectivities in the CO2 hydrogenation reaction for the ligand exchanged MoC1–x nanoparticle catalysts and the as-prepared material.

Thermal-Driven Formation of Silver Clusters Inside Na/Li FAUY Zeolites for Formaldehyde Detection.

In this research, the LiY zeolite was firstly synthesized by using NaY as the parent zeolite; thereafter, the LiYAg and NaYAg zeolites created for formaldehyde gas detection were prepared with further Ag+-Li+/Na+ exchange and a mild thermal treatment at 300 °C to promote the formation of luminescent Ag CLs. The spectra experimental results indicated that Ag CLs showed stronger and blue-shifted emissions in LiYAg compared with in NaYAg, and the emission intensity of Ag CLs in both zeolites monotonously decreased when exposed to increasing formaldehyde gas content. Moreover, the linear dependence of the Ag CLs’ emission intensity variation on formaldehyde content indicated a reliable method for fast and sensitive formaldehyde detection. According to the XPS, UV–vis absorption, and N2 adsorption–desorption isotherm studies, the formaldehyde-gas-induced luminescence quenching of Ag CLs is due to the formation of Ag2O and Ag NPs, in which the higher content of Ag+/Ag0 redox couples in LiYAg and larger surface area of NaYAg benefit the precise detection of formaldehyde gas in low- and high-content ranges, respectively. Furthermore, the blue-shifted peak position and widened FWHM of Ag CLs can also be used for the indication of formaldehyde gas and the detection limit of NaYAg and LiYAg, which both meet with the standards of the WHO and OSHA.

Stability of phenolic compounds in apple and strawberry: Effect of different processing techniques in industrial set up

Different technologies commonly employed by the fruit processing industry affect the phenolic content. This study aimed to establish the extent to which different processing techniques and conditions, tested at an industrial scale, affect phenolics, color, and sensory attributes of fresh strawberries and apples. The effects of freezing, thermal treatments, and high-pressure processing were investigated. In strawberries, mild and standard thermal treatments showed similar patterns for most phenolic groups; an increase in proanthocyanidins, no change in ellagic acid conjugates, and a major decrease in flavonols and anthocyanins. In apples, mild treatments and high-pressure processing had similar effects in all phenolic groups, with increases in dihydrochalcones, hydroxycinnamics, and proanthocyanidins and decreases in flavonols. However, the standard thermal treatment increased flavonols and dihydrochalcones concentrations. This study shows that each fruit behaves differently, and both technology and processing conditions should be customized accordingly to preserve or even increase the phenolic content.

Reversible and irreversible effects of mild thermal treatment on the properties of wood used for making musical instruments: comparing mulberry to spruce

Thermal treatments can be considered as an accelerated ageing, bringing partly similar changes in properties as naturally aged wood. Thermal treatment was applied on white mulberry (Morus alba L.), a dominant species for making musical instruments from middle-East to Far-East, to investigate the effects on the vibro-mechanical and physical properties of this wood, and the results compared to previously published data on spruce (Picea abies Karst.) as a reference for the soundboard of Western string instruments. Thermal treatment (TT) at 150 °C and 0% of relative humidity was applied to five analogous groups of specimens with five different durations (2.5, 8, 24, 72, 261 hours). Humidity re-conditioning of specimens was done to explore the reversibility of TT effects. Physical and vibrational properties such as specific gravity (γ), equilibrium moisture content (EMC), CIELab colorimetric values, specific modulus of elasticity (E’/γ) and damping coefficient (tanδ) in longitudinal (L) and radial (R) directions, have been measured after stabilisation of samples in standard conditions (20 °C, 65% RH), before and after TT and then after re-conditioning. Untreated mulberry had a low EMC, very low L/R anisotropy and low E’L/γ, and relatively low tanδ. Weight loss (WL) and CIELab values evolved similarly during TT for mulberry and for previous results on spruce, however, their EMC and vibrational properties were affected differently. This could be explained in part by the low anisotropy of mulberry, and in part by its particular extractives. The parts of irreversible effects, linked to chemical modification or degradation, and of reversible effects, linked to physical configuration, were different between mulberry and spruce. The applied treatments did not bring permanent “improvements” in vibrational properties of mulberry, yet its colour appearance was enhanced.

Evaluation of Potential for Butyl and Heptyl Para-Hydroxybenzoate Enhancement of Thermal Inactivation of Cronobacter sakazakii during Rehydration of Powdered Infant Formula and Nonfat Dry Milk

In previous studies, parabens in model systems enhanced the thermal inactivation of foodborne pathogens, including Cronobacter sakazakii, Salmonella enterica serotype Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes. However, few studies have been conducted to evaluate this phenomenon in actual food systems. In the present study, the potential enhancement of thermal inactivation of C. sakazakii by butyl para-hydroxybenzoate (BPB) was evaluated in powdered infant formula (PIF) and nonfat dry milk (NFDM) in dry and rehydrated forms. When PIF was rehydrated with water at designated temperatures (65 to 80°C) in baby bottles, BPB did not enhance thermal inactivation. When rehydrated NFDM and lactose solutions with BPB were inoculated and heated at 58°C, BPB enhancement of thermal inactivation of C. sakazakii was negatively associated with the concentration of NFDM solutions in a dose-dependent manner, whereas thermal inactivation was enhanced in the presence of lactose regardless of its concentration, suggesting an interaction between proteins and BPB. Fluorescence testing further indicated an interaction between BPB and the proteins in PIF and NFDM. In inoculated dry NFDM with and without BPB stored at 24 and 55°C for 14 days, BPB did not substantially enhance bacterial inactivation. This study suggests that BPB is not likely to enhance mild thermal bacterial inactivation treatments in foods that have appreciable amounts of protein.

Torrefaction Process of Needles, Cones and Bark of Spruce (Picea Abies (L.) Karst) and Pine (Pinus Sylvestris L.)

The actual problems related with the depletion of fossil resources and the accentuated increase of global warming requires rapid shift to renewable and eco-friendly alternatives. Biomass forestry residues are valuable candidates that can be used as sustainable resources. Torrefaction is a mild thermal treatment that can convert biomass into value-added liquid and solid products. Here we performed torrefaction at 250 �C of needles, cones and bark from spruce and pine, as representative coniferous species in Romanian Carpathians. The elemental analysis of solid materials was performed and the calculated O/C and H/C atomic ratios showed that torrefaction placed the solid materials in the region of coals, close to the origin of the Van Krevelen diagram. The calorific values were improved, leading to higher energy density. The energy yield was close to the unit, indicating that torrefaction is a highly efficient process. The liquid products had complex composition, with compounds coming from the compositional and structural changes in the forestry residues. Terpenes are the dominating compounds from volatile extractives. Furans, from which furfural and 5-methylfurfural were the main compounds produced from degradation of hemicelluloses, while phenol derivatives were produced from lignin. Exploratory data analysis revealed diferences between samples from spruce and pine but also between needles, cones and bark.

Simulated discharge overpotential distributions for sintered electrode batteries in rechargeable coin cell form factors

Sintered electrode lithium-ion batteries contain only electroactive material which has undergone a mild thermal treatment to improve particle connectivity and thus electrode mechanical strength. These electrodes can be made very thick, and due to the lack of inactive binders or conductive additives there are ion transport advantages relative to equivalent thickness and porosity composite electrodes. In this study, the discharge of sintered electrode full cells was simulated at the cell level for three different form factors: CR2032, CR2025, and CR2016. Simulations also include relatively high and low rates of discharge and two different electrolytes. Using the simulations, overpotential distributions enable analysis of the contributors to resistance in the cell analyzed for the different regions of the anode, cathode, and separator. Overpotential sources include electronic overpotential, ionic overpotential, interfacial overpotential, and OCV overpotential. As the total thickness increases, the main overpotential contribution shifts from interfacial to ionic, with both originating from the electrodes. In all cases a higher conductivity electrolyte dramatically increases total capacity delivered at higher discharge rates.

Oxidative condensation/esterification of furfural with ethanol using preformed Au colloidal nanoparticles. Impact of stabilizer and heat treatment protocols on catalytic activity and stability

The oxidative condensation of furfural and ethanol has been studied using supported gold colloidal nanoparticles. The influence of the nature of stabilizer (polyvinylalcohol, polyvinylpyrrolidone), the choice of thermal treatment and washing for removing the stabilizer, on the catalytic performance has been evaluated. Variation of the mean gold particle size and surface coverage of the gold nanoparticles onto the support surface were achieved by modifying the stabilizer to Au weight ratio. Thus, the mean gold particle size was varied in the range of 3–8 nm. The catalytic results showed that the choice of stabilizer, the stabilizer to Au weight ratio, thermal treatment of the catalyst, affect catalytic activity and selectivity/yield to the desired products. When PVA was the chosen stabilizer, the highest yield to furan-2-acrolein (33%) was attained with a PVA to Au weight ratio of 2.4. On the contrary, when PVP was the stabilizer, the best catalytic performance was achieved in its absence, with the yield of furan-2-acrolein reaching 21%. These results showed the strong impact of stabilizer to control catalytic activity and to enhance, in the case of PVA, the yield to the target product, whereas a strong negative effect was observed with PVP. Moreover, a mild thermal treatment of the catalyst and a washing step for the removal of the stabilizer from the catalyst had a positive effect on the catalytic performance.

Flexible and Transparent Electrode of Hybrid Ti3C2TX MXene-Silver Nanowires for High-Performance Quantum Dot Light-Emitting Diodes.

The development of electrodes with high conductivity, optical transparency, and reliable mechanical flexibility and stability is important for numerous solution-processed photoelectronic applications. Although transparent Ti3C2TX MXene electrodes with high conductivity are promising, their suitability for displays remains limited because of the high sheet resistance, which is caused by undesirable flake junctions and surface roughness. Herein, a flexible and transparent electrode has been fabricated that is suitable for a full-solution-processed quantum dot light-emitting diode (QLED). An MXene-silver nanowire (AgNW) hybrid electrode (MXAg) consists of a highly conductive AgNW network mixed with solution-processed MXene flakes. Efficient welding of wire-to-wire junctions with MXene flakes yields an electrode with a low sheet resistance and a high transparency of approximately 13.9 Ω sq-1 and 83.8%, respectively. By employing a thin polymer buffer layer of poly(methyl methacrylate) (PMMA), followed by mild thermal treatment, a hybrid PMMA-based MXene-AgNW (MXAg@PMMA) electrode in which the work function of an MXAg hybrid FTE physically embedded in PMMA (MXAg@PMMA) can be tuned by controlling the amount of MXene in the hybrid film facilitates the development of a high-performance solution-processed QLED that exhibits maximum external quantum and current efficiencies of approximately 9.88% and 25.8 cd/A, respectively, with excellent bending stability. This work function-tunable flexible transparent electrode based on solution-processed nanoconductors provides a way to develop emerging high-performance, wearable, cost-effective, and soft electroluminescent devices.

In situ crosslinkable acrylic random copolymer binders for silicon anodes in lithium‐ion batteries

Silicon is in the spotlight as a promising anode material for next-generation Li-ion batteries. However, its practical application is limited by a rapid decay in capacity due to considerable volume changes. Rational design of binders that can exhibit strong adhesion and robust mechanical properties is crucial for overcoming such challenges. Herein, we synthesize a series of multifunctional acrylic random copolymers comprising various ratios of acrylic acid and glyceryl groups by free-radical polymerization. The adhesive and mechanical properties of the binders are systematically modulated, and their impact on the electrochemical properties of a Si-nanoparticle anode are investigated. Owing to the presence of both acrylic acid and hydroxyl groups, the copolymers containing 7 mol% and 25 mol% glyceryl group undergo in situ crosslinking under mild thermal treatment (70°C for 24 h). In particular, the copolymer containing 7 mol% glyceryl group exhibits the highest specific capacity and capacity retention (1170 mAh g−1 and 54% after 100 cycles at 0.5 A g−1, respectively), which is superior to those of a conventional poly(acrylic acid) binder (880 mAh g−1 and 52% after 100 cycles at 0.5 A g−1). The enhanced electrochemical properties attribute to the improved adhesive and mechanical properties enabled by the network structure.

Positive Influences of Ohmicsonication on Phytochemical Profile and Storage Stability of Not-from-Concentrate Mango Juice.

Processing technique and storage conditions are the main factors that affect the phytochemical profile of Not-from-Concentrate (NFC) juice, which could decrease the nutritional and bioactive properties of the corresponding juice. The aim of this study was to evaluate the quality changes that occurred in NFC mango juice after Ohmicsonication (OS) and during storage in comparison to other processing methods such as sonication (S), thermosonication (TS), ohmic heating (OH), and conventional heating (CH). Quality attributes such as polyphenoloxidase (PPO) and pectinmethylesterase (PME) activities, ascorbic acid and hydroxymethyl furfural (HMF) contents, total phenolics, total flavonoids, total carotenoids, electric conductivity, color values and microbial load (total plate count, mold, yeast, and psychrophilic bacteria) were examined. OS and OH treatments demonstrated the highest inactivation of PPO (100%), while CH and TS displaying inhibitions 89% and 90%, respectively and only S treatment exhibited insufficient inactivation of both PPO and microbial load. However, the inhibition of PME followed the order OS (96.5%) > OH (94.9%) > TS (92.5%) > CH (88.5%). The best treatment, with the highest retention of phytochemical contents (ascorbic acid, total carotenoids, antioxidant activity, total flavonoids, and total phenolic content) for NFC mango juice and during storage was obtained with OS treated samples compared to other treatments (in the order from the lowest to highest percentage, OS < OH < TS < CH). Consequently, the results indicated that OS could be applied as a new mild thermal treatment in the production of mango juice with improved quality properties of stored NFC mango juice.