Human metapneumovirus: Insights into immune evasion, pathogenesis and liposome-based vaccine strategies.

Introduction: Tolerance and dependence are known to occur with prolonged cannabis use. Few animal experiments on spontaneous withdrawal from delta-9-tetrahydrocannabinol (THC), the primary psychoactive compound in cannabis, have been conducted. The experiments that have been conducted use extremely high doses and report relatively mild symptoms. The objective of the present experiment was to determine the magnitude and duration of spontaneous THC withdrawal in male and female rats using voluntary home cage wheel running as a sensitive, objective, and continuous measure of withdrawal. Materials and Methods: Male and female Sprague-Dawley rats were injected with THC (3 mg/kg, s.c.) or vehicle twice daily for 7 days to induce tolerance and dependence. Spontaneous withdrawal was assessed for 5 days beginning 24 h after the day 8 tolerance test in which all rats received a THC injection. Results: On day 1, THC profoundly decreased wheel running in male and female rats compared to vehicle-treated rats. On day 8, THC given to vehicle-treated rats produced a significantly greater decrease in running than in rats previously treated with THC, indicating tolerance development. There was no sex difference in the magnitude of the decrease in wheel running caused by THC injections or in the development of tolerance. There were no significant changes in wheel running associated with spontaneous THC withdrawal. Discussion: These data suggest that spontaneous THC withdrawal in male and female rats is mild at best despite profound locomotor suppression and tolerance to repeated injections. The lack of spontaneous THC withdrawal contrasts sharply with the pronounced changes in wheel running produced by opioid withdrawal.

Background: Nowadays, one of the best ways to manage COVID-19 is to understand the characteristics of patients and the risk factors associated with the disease. Objective: This study aimed to investigate and compare the characteristics, symptoms, and prognosis of inpatients and outpatients with COVID-19 in Poledokhtar city Methods: In this descriptive-analytical study, 288 patients who tested positive for COVID-19 through PCR tests at COVID-19 screening centers and Imam Khomeini Hospital in Poledakhtar city were selected using the census method. They were divided into inpatient and outpatient groups. Data from patient files were collected and analyzed using SPSS version 25 software. Results: The study revealed significant differences in age, comorbidities, prior infection, and aspirin use between the inpatient and outpatient groups. Cough was the most common symptom among all patients, with weakness and fatigue being more common in inpatients and cough being more prevalent in outpatients. The overall mortality rate was 5.2%, with inpatients having a worse prognosis compared to outpatients. Conclusion: In the present study, inpatients were found to be older, have more underlying health conditions, fewer prior infections, and experience a poorer prognosis than outpatients. Given that patients may be asymptomatic or have mild symptoms upon admission, evaluating other patient factors is crucial in assessing disease severity.

Quantification of Zika virus using a colloidal gold nanoparticle-based immunosensor and Fourier-transform infrared spectroscopy.

Aversive light sensitivity and aging: Implications for sleep and health in older adults.

Vulnerability and core intervention targets in the symptom burden for personalized symptom management in hemodialysis care: A computational simulation modeling study.

Background. Immune checkpoint inhibitors (ICIs), widely used in cancer immunotherapy, can trigger immune-related adverse events, including polymyalgia rheumatica (PMR) and giant cell arteritis (GCA), likely through mechanisms involving altered immune tolerance. We conducted a systematic review (SLR) and meta-analysis to characterize these conditions and explore potential differences from the idiopathic forms. Materials and Methods. The SLR analyzed Medline and EMBASE databases up to July 2024, comparing ICI-induced PMR and GCA to their primary forms. Where direct comparisons with idiopathic forms were unavailable, we referred to data from prior meta-analyses and large observational cohorts to qualitatively explore potential differences. Results. From 1,237 abstracts, 46 were included, yielding 358 patients (314 with ICI-PMR and 44 with ICI-GCA). ICI-PMR had a pooled prevalence of 0.3% [95% CI: 0.1%–1.2%] among ICI recipients. Patients were predominantly males (64% [95% CI: 54%–73%]), with a mean age of 71 years [95% CI: 68–74]. PD1/PDL1 blockers were used in 85% [95% CI: 80%–89%] of cases. Inflammatory pain in the girdles was universal (100%), however pelvic girdle involvement was explicitly reported in only 3 studies. Peripheral arthritis in ICI-PMR was present in 26% [95% CI: 9%–54%], and normal inflammatory markers were detected in 26% [95% CI: 15%–40%]. Glucocorticoids (GCs) completely improved symptoms in 83% of patients [95% CI: 66%–92%], with 13% [95% CI: 12%–34%] requiring DMARDs and 18% [95% CI: 9%–33%] experiencing relapses (Figure 1). Two comparative case-control studies showed significant differences between ICI-induced and primary PMR. ICI-PMR patients had milder symptoms, lower C-reactive protein levels, and required lower median prednisone dosages. ICI-GCA prevalence was 0.06% among ICI recipients. Male patients comprised 51% [95% CI: 36%–66%], with a mean age of 71 years [95% CI: 68–74]. About 50% [95% CI: 23%–77%] received anti-CTLA4 blockers (alone or with PD1 blockers), while the rest received PD1/PDL1 blockers. Clinical features included cephalic symptoms (85% [95% CI: 70%–93%]), permanent visual loss (23% [95% CI: 12%–39%]), and large-vessel involvement (62% [95% CI: 40%–80%]). No comparative studies between ICI-GCA vs primary GCA were retrieved. High-dose GCs permitted remission in 95% [95% CI: 73%–99%], though 19% [95% CI: 7%–41%] experienced relapses and 10% [2% - 31%] required DMARDs (Figure 1). Conclusions. ICI-induced PMR and GCA may differ from idiopathic forms, potentially presenting with milder symptoms and more favorable treatment responses. Large observational data and meta-analyses on primary forms suggest higher relapse rates and prolonged GC use [1,2], yet further robust comparative studies are needed to validate whether ICI-induced syndromes truly represent a distinct and less severe clinical entity.

BackgroundThis cross-sectional study aims to describe the characteristics of physical activity, sedentary time, sleep quality, and resting EEG among college students with mild depressive symptoms, and further explore pairwise correlations between behavioral patterns, resting EEG, and mild depressive symptoms.MethodsThis study included 75 college students with mild depressive symptoms (MDS) and 75 college students without depressive symptoms (ND) as research subjects. Physical activity (vigorous physical activity (VPA), moderate physical activity (MPA), and low physical activity (LPA)) and sedentary time（ST） were measured using the International Physical Activity Questionnaire Short Form (IPAQ-SF). Sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI). Resting EEG power values were collected from subjects in a quiet, eyes-closed state using an electroencephalography (EEG) device.Results(1) Characteristic analysis revealed that compared with the ND group, the MDS group exhibited reduced MPA and VPA scores, elevated ST scores, and increased total PSQI scores along with elevated scores across its subdimensions. Their behavioral patterns (Moderate-to-Vigorous Physical Activity (MVPA), Sedentary Behavior (SB), Poor Sleep Quality (PSQ) may have changed, including a decrease in the proportion of MVPA, an increase in the proportion of SB, and an increase in the proportion of PSQ. Analysis of resting EEG revealed increased Alpha2 (α2) band power in the temporal regions (T3 and T5) and increased Beta1 (β1) band power in the frontal region (Fp1) in the MDS group (all pFDR < 0.01). (2) Exploratory analyses revealed that SB and PSQ behaviors may be positively correlated with mild depressive symptoms, while MVPA may be negatively correlated with mild depressive symptoms. The combination of certain EEG indicators (α2 at T3 and T5, β1 at Fp1) achieved an AUC of 0.659 (95% CI: 0.572 to 0.745, p = 0.001) for identifying mild depressive symptoms. Additionally, in the MDS group, sleep efficiency scores showed a negative correlation with Alpha1 (α1) band power at the frontal region (F4) electrode position, sleep disturbance scores showed a negative correlation with theta (θ) band power at the parietal region (P3), hypnotic medication scores showed a positive correlation with beta1 (β1) band power at the frontal region (F3), and total PSQI scores showed a negative correlation with θ band power at the temporal region (T3).ConclusionCollege students with mild depressive symptoms may exhibit altered behavioral patterns and abnormal neural activity in the frontal and temporal regions. Their changed behavioral patterns may correlate with mild depressive symptoms, and recognition models based on certain resting EEG indicators demonstrate preliminary application potential. The association between specific sleep issues and localized EEG activity in this population may provide evidence for further elucidating the mechanistic pathways linking their behavior and brain activity. Future longitudinal studies are recommended to explore causal relationships among these variables.

More than one-third of inguinal hernias are asymptomatic, and performing surgery in the absence or presence of mild symptoms remains a point of concern for many surgeons. This study aims to evaluate whether watchful waiting (WW) is a viable management strategy for asymptomatic and mildly symptomatic inguinal hernias by comparing its outcomes to early surgical repair. To broaden the scope of existing evidence, we included both recent randomized controlled trials and observational studies. PubMed, Cochrane Central, and EMBASE were searched for studies comparing early repair and WW in patients with asymptomatic or mildly symptomatic inguinal hernias. The primary outcomes assessed were the rate of crossover from WW to surgery, reasons for crossover, and the presence of pain in both groups. Secondary outcomes included quality of life (QoL) and hernia recurrence. A Kaplan-Meier failure function curve was constructed to estimate time from study enrollment to crossover. Among 619 screened studies, 12 met the inclusion criteria, covering 1,755 patients (748 (42.6%) in early surgical intervention and 1,007 [57.4%] in the WW). Among WW patients, 477 (27.1%) eventually crossed over to surgery, with crossover rates ranging from 6.1% (Patti 2014) to 75.4% (Chung 2011). Approximately 50% of WW patients required surgery within five years. The most reported reason for crossover was pain (65%), followed by impaired quality of life (16%), bowel-related symptoms such as incarceration or strangulation (8.5%), hernia volume progression (3%), and other or unspecified causes (7.5%). Pain was the most consistent patient-specific predictor of crossover to surgery. WW appears to be a viable and safe approach with low complications rates for patients with asymptomatic inguinal hernias. However, long-term data show that approximately 50% of patients cross over to surgery within five years, and this proportion exceeds 96% by 12years. Ultimately, the decision between WW and early surgical intervention should be guided by shared decision-making, considering patient values, symptom burden, comorbidities, and life expectancy.

BackgroundRickettsia spp. are intracellular, arthropod-borne bacteria pathogenic to humans and animals. The clinical manifestations of rickettsial infections range from mild to severe, but diagnosis is sometimes missed owing to mild symptoms or empirical antibiotic treatment for suspected tick-borne diseases. This study aimed to determine the prevalence of Rickettsia spp. in ticks across Finland, explore possible regional variations, identify the infecting Rickettsia species, and assess rickettsial exposure of certain human patients.MethodsWe analysed 5101 ticks from 20 locations, collected by flagging, crowdsourcing, or removal from pet cats and dogs between 2003 and 2021 in three study sets. Tick species were determined by quantitative PCR (qPCR), Sanger sequencing, or morphology. Rickettsial prevalences were determined by qPCR, with species confirmed by Sanger sequencing. Indirect immunofluorescence assay was used for detecting Rickettsial antibodies in human samples.ResultsIxodes ricinus predominated in southern Finland, while Ixodes persulcatus was found in central Finland and along the western coast up to southern Lapland. Occasional I. ricinus–persulcatus hybrids were observed in central southern Finland where both species co-occurred. Rickettsia DNA was more frequently detected in ticks collected from regions dominated by I. ricinus compared to those dominated by I. persulcatus. However, variations in sampling and processing may limit the comparability of these results. Ticks collected from dogs and cats contained Rickettsia spp. more frequently than those collected by flagging. Rickettsia helvetica was the primary species identified, with sporadic Candidatus Rickettsia tarasevichiae found exclusively in I. persulcatus. Among 226 sera from patients suspected of tick-borne encephalitis, 5.8% showed IgG reactivity against Rickettsial antigens, but titres were below the 1:512 threshold for confirmed infection.ConclusionsThis study highlights the need to better understand environmental or host-linked factors influencing Rickettsial prevalence and emphasizes the importance of monitoring in areas prone to tick expansion due to climate change. The findings underscore the potential for Rickettsial diseases, necessitating enhanced diagnostic frameworks to address disease burden and improve surveillance.Graphical Supplementary InformationThe online version contains supplementary material available at 10.1186/s13071-025-07090-6.

Objective: Nausea and vomiting of pregnancy (NVP) are frequently encountered in pregnancy and can lead to serious maternal and fetal complications if neglected. This study aims to determine the frequency of NVP and related factors in Rize. Material and Methods: In this prospective study, the severity and frequency of NVP were determined by scoring pregnant women between 5-20 weeks of gestation, using the Pregnancy-Unique Quantification of Emesis and Nausea (PUQE) scoring system. Pregnant women with a PUQUE score below 7 were defined as the mild NVP group, and a score of 7 and above was defined as the moderate and severe NVP group. Demographic data and blood electrolyte levels were compared between the two groups. Results: A total of 221 pregnant women were included. The frequency of moderate-severe NVP was found to be 10% (n = 22). The frequency of hyperemesis gravidarum was 2% (n=4). The average body mass index of pregnant women with moderate-severe NVP symptoms was found to be higher than that of pregnant women with mild symptoms (p&amp;lt;0.001). The average blood sodium and potassium levels of pregnant women with moderate-severe NVP were found to be lower (p=0.003 and &amp;lt;0.001). Conclusion: The frequency of NVP in Rize province is similar to the literature. Increased body mass index may be associated with the frequency of NVP. Electrolyte imbalances may occur in the blood of these pregnant women. For this reason, all pregnant women should be carefully evaluated in terms of NVP, and treatments should be planned to eliminate electrolyte imbalances in patients.

Large-scale analyses of the relationship between eyewash and hay fever symptoms after adjusting for factors associated with allergic symptoms have not yet been conducted. We aimed to evaluate the association between eyewash and ocular symptoms of hay fever. In this digital prospective observational cohort study, we used the AllerSearch smartphone application. Participants reported symptoms (eye itching, tearing, and redness) and their preventive behaviors such as eye washing. Multivariate regression analysis was used to determine the association; temporal changes in ocular symptoms were compared using a mixed-effects model. Of 476 included participants, 71 practiced eye washing. Eyewash group had milder symptoms than non-eyewash group (estimated difference between the two groups, 95% confidence interval): eye itching (− 0.851 points, − 1.369 to − 0.333, P = 0.001), tearing (− 0.655 points, − 1.217 to − 0.094, P = 0.022), total ocular symptoms score (− 0.840 points, − 1.481 to − 0.198, P = 0.010), and total Japanese Allergic Conjunctival Disease Quality of Life Questionnaire score (− 5.983 points, − 9.633 to − 2.333, P = 0.001). Longitudinal analysis indicated that eyewash group maintained milder ocular symptoms over time, with more symptom-free days. In conclusion, eye washing was associated with milder ocular symptoms of hay fever.Supplementary InformationThe online version contains supplementary material available at 10.1038/s41598-025-24950-4.

The emergence of SARS-CoV-2 has posed significant threats to global health, particularly for the older population. Similarly, common human coronaviruses, such as HCoV-229E, which typically cause mild cold-like symptoms, can lead to severe diseases, underscoring the need to understand virus-host interactions and identify host factors contributing to viral pathogenesis and disease progression. In this study, we perform a genome-wide CRISPR knockout screen using HCoV-229E and identify UHRF1 as a potent restriction factor. Mechanistically, UHRF1 suppresses HCoV-229E infection by downregulating the expression of its cell entry receptor, APN, through promoter hypermethylation. Focused CRISPR activation screens of UHRF1-downregulated genes confirm the critical role of APN in HCoV-229E infection and identify additional genes (e.g., SIGLEC1, PLAC8, and heparan sulfate biosynthesis genes) contributing to the restrictive functions of UHRF1. Transcriptomic and single-cell RNA sequencing analysis reveal that UHRF1 expression decreases with age, negatively correlating with increased APN expression. This age-related decline in UHRF1 is validated in primary alveolar macrophages from elderly individuals, which exhibit heightened susceptibility to HCoV-229E compared to those from younger individuals. Our findings highlight UHRF1 as a key age-related host defense factor against coronavirus and provide insights into the epigenetic regulation of viral entry receptors.

Background: Autism Spectrum Disorder (ASD) is a complex and heterogeneous neurodevelopmental condition. Diagnosing ASD in adults, especially in milder forms, remains challenging due to camouflaging strategies, adaptive behaviors, and frequent psychiatric comorbidities. Despite increased awareness, there is a critical need to improve recognition and tailored interventions for adults with ASD. This study aims to examine the prevalence of psychiatric comorbidities among individuals diagnosed with ASD. Methods: This retrospective cross-sectional study examined 64 adults diagnosed with ASD (n = 29 females, 45.3%; age: range, 18–57 years; mean ± SD, 30.9 ± 8.92), who accessed two university hospital outpatient units in Rome between September 2023 and January 2025. All participants were assessed using the Autism Diagnostic Observation Schedule, Second Edition–Module 4 (ADOS-2). Psychiatric comorbidities were evaluated using clinical assessments and the Mini-International Neuropsychiatric Interview (M.I.N.I.) Plus. Results: All patients received an ASD diagnosis without intellectual disability. Forty-four (68.8%) presented with at least one psychiatric comorbidity, most commonly depressive (25.0%) and anxiety disorders (9.4%). Over half of the participants (57.4%) reported at least mild depressive symptoms, and 42.6% exhibited moderate to severe depressive levels. Conclusions: High rates of psychiatric comorbidities, particularly mood and anxiety disorders, were observed, underscoring the importance of comprehensive, multidisciplinary assessment and individualized interventions. Further research using larger samples and rigorous methodologies is warranted to better characterize the ASD phenotype in adults and guide targeted therapeutic strategies.

Relevance . Acute respiratory infections (ARI) are a leading cause of morbidity and mortality in children worldwide. The COVID-19 pandemic has significantly influenced the circulation of other respiratory viruses. Understanding the interactions between SARSCoV-2 and other respiratory pathogens is crucial for predicting disease severity, improving diagnostic and treatment methods, and developing effective prevention strategies. Aims. This study aims to analyze changes in the circulation of respiratory viruses at different stages of the COVID-19 pandemic, investigate the prevalence and characteristics of viral and bacterial co-infections in children, and assess their impact on disease progression. Conclusions. At the initial stage of the COVID-19 pandemic, the incidence of respiratory viral infections declined, followed by a rapid resurgence after the lifting of restrictive measures. In children, COVID19 presents with milder symptoms than in adults and has a high proportion of asymptomatic cases. Mixed SARS-CoV-2 infections with other respiratory viruses are more common in younger children and do not significantly increase disease severity. However, secondary bacterial infections in children with COVID-19 substantially elevate the risk of severe disease progression.

Previous studies have shown that receiving Assisted Reproductive Technology (ART) treatment can affect women's mental health negatively. Using prospective cohort data of women undergoing ART treatment in Japan, we investigated the associations of depression symptoms at the early stage of ART treatment with (1) the likelihood of achieving pregnancy and (2) depression symptoms at the time of reporting pregnancy during the follow-up period. Our analysis included 338 women. Our exposure variable was depressive symptoms at the early stage of ART treatment, assessed with Quick Inventory of Depressive Symptomatology-Self report. We examined the associations with two outcomes: (1) archiving pregnancy and (2) depression symptoms, assessed with Edinburgh Postnatal Depression Scale, at the time of reporting pregnancy. We conducted Poisson regression analysis to examine the associations, controlling for potential confounders. We observed null association between depressive symptoms at the early stage of the treatment and the likelihood of achieving pregnancy. However, women with mild or more severe depressive symptoms were more likely to exhibit depressive symptoms at the time of reporting their pregnancy [Incidence Rate Ratio = 3.11 (95% confidence intervals: 1.66-5.84) with the mild symptom group]. Null association of depressive symptoms at the early stage of treatment with the likelihood of achieving pregnancy may provide reassurance to women receiving ART treatment, alleviating some of the possible mental distress. However, a greater risk of showing depressive symptoms at the time of reporting pregnancy highlights the importance of early detection and support for women's mental health during the ART treatment.

Longitudinal monitoring of cellular immunity by the ex vivo activation of genes in leukocytes (EAGL) assay highlights potential markers of mRNA COVID-19 vaccine efficacy against breakthrough symptoms.

It is unclear whether a hip with a developmental dysplasia deformity can remain functional and free of osteoarthritis (OA) throughout life. This study aims to determine the percentage of Chinese older adults without severe OA who meet the diagnostic criteria for DDH and BDDH. Additionally, this study seeks to explore hip morphology in this population. Data from 808 consecutive patients with recent unilateral femoral neck fractures, collected between January 2022 and October 2024, were retrospectively analyzed. A total of 493 patients (493 hips) were included in the analysis. For imaging evaluation, the following parameters of the contralateral (unfractured) hip were measured: LCEA, Tönnis angle, Sharp's angle, femoral head eminence index (FHEI), and femoral head lateralization. Categorical variables were expressed as numbers and percentages. Continuous variables were presented as mean ± SD if normally distributed; otherwise, they were reported as median (Q1, Q3). The Pearson chi-square test, likelihood ratio chi-square test, or Fisher's exact test was used to compare categorical variables. An independent-samples t-test or Mann-Whitney U test was used to compare continuous variables in the group analysis. For parameter comparisons between multiple groups, use ANOVA with post hoc analysis. In this study, 7.1% of individuals aged over 60 years with unilateral femoral neck fractures exhibited imaging results consistent with DDH, while 13.2% had results consistent with BDDH. Patients with DDH and BDDH were at a higher risk of developing mild OA compared to individuals with normal acetabular coverage. However, not all individuals with DDH or BDDH develop OA. Only the Tönnis angle was significantly associated with mild OA, indicating a 7.8% increase in OA risk for each 1° increase in the Tönnis angle. Significant differences were observed in the Tönnis angle (5.0 ± 3.9 vs. 11.5 ± 4.1 vs. 17.5 ± 4.1, p < 0.001), Sharp's angle (38.2 ± 2.9 vs. 41.7 ± 2.5 vs. 44.0 ± 2.5, p < 0.001), femoral head lateralization (7.8 ± 2.7 vs. 9.3 ± 2.6 vs. 10.3 ± 2.8, p < 0.001), and FHEI (17 ± 4 vs. 25 ± 3 vs. 30 ± 4, p < 0.001) between the normal group and both the DDH and BDDH groups. The prevalence of DDH imaging abnormalities is notable among Chinese older adults without severe OA. Individuals with DDH and BDDH are more likely to exhibit mild OA symptoms, although not all develop OA. Using multiple imaging parameters in addition to LCEA facilitates characterizing hip morphology in asymptomatic individuals with DDH.

Background: Heart failure (HF) is the most common complication in patients with obstructive hypertrophic cardiomyopathy (oHCM), significantly impairing quality of life and prognosis. In asymptomatic or mildly symptomatic patients, reliable markers for HF progression are lacking. Early identification could improve counseling and support timely consideration of gradient-reducing therapies. Although potentially important, the role of the right ventricle (RV) in HF progression in oHCM remains poorly understood. This study evaluated RV parameters by cardiac magnetic resonance (CMR) as early predictors of HF risk beyond conventional metrics in oHCM. Methods: In a multi-center cohort of 3134 patients with HCM who underwent CMR between 2003 and 2023, we identified 771 mildly or asymptomatic (NYHA I/II) patients with oHCM, rest or provoked gradients ≥30 mmHg, LVEF ≥50%, and no prior septal reduction therapy (SRT). CMR-derived biventricular volumes were indexed to BSA, LGE was manually quantified. The primary outcome was a composite “HF Event”: progression to NYHA Class III/IV, SRT, new LVEF&lt;50%, HF-related death, or heart transplantation. To further investigate predictors of newly emerging HF symptoms, a secondary analysis was performed in the subgroup of 347 asymptomatic patients (NYHA class I), using the composite endpoint “HF Progression”: new HF symptoms (NYHA class ≥II), SRT, new LVEF&lt;50%, HF-related death, or heart transplantation. Uni and multivariable Cox regression analyses were performed, and Kaplan–Meier plots visualize the prognostic relevance of RV stroke volume index (SVI) at an optimal cutoff determined by Youden’s index. Results: Over a mean follow-up of 3.6±3.0 years, 216 (28%) patients experienced HF Events. These patients were older, more often female, had higher BMI and outflow gradients, more intense medical treatment, and smaller RV volumes and RVSVI ( Table 1 ). In regression analyses, lower RVSVI emerged as an independent predictor of HF Events ( Figure 1A ). Among asymptomatic patients, 98 (28%) experienced HF Progression over a mean follow-up of 4.0±3.0years. Female sex, atrial fibrillation, and lower RVSVI were independent predictors of HF progression in this subgroup ( Figure 1B ). Conclusion: In oHCM patients with no or mild symptoms, lower RVSVI emerged as an independent predictor of future HF symptoms and adverse HF events. These findings highlight the underrecognized prognostic relevance of the RV in oHCM.

This study aimed to explore, characterize, and compare mental health profiles of women in peri- and post-menopause. A sample of 577 women aged between 45 and 55 in perimenopause (66.6%) and postmenopause (33.4%) participated in an online survey. A latent profile analysis was conducted to estimate the number of profiles, based on anxiety symptoms, depressive symptoms and positive mental health. Differences in menopause-related, sociodemographic, and clinical data between profiles were examined. Four mental health profiles were identified: impaired mental health (IMH; elevated depressive and anxiety symptoms and low positive mental health); threatened mental health (TMH; mild depressive and anxiety symptoms and low positive mental health); moderate mental health (MMH; low depressive and anxiety symptoms, and moderate positive mental health); complete mental health (CMH; low depressive and anxiety symptoms and high positive mental health).Significant differences between profiles were found, with women in the IMH profile reporting the highest levels of menopausal symptoms, insomnia severity, lower sexual functioning, and greater negative impact on work life, romantic relationships, and overall health and well-being. Women in the CMH profile were more likely to be employed and to have higher incomes compared to other profiles. Differences were also observed in the history of psychopathology, current professional care for peri- and post-menopausal symptoms and physical exercise practice. These findings provide evidence for diverse patterns of mental health during transition to menopause, with important implications for screening and intervention, including the development of tailored psychological interventions.