Significant discoveries in deeply buried marine carbonate petroleum exploration have been made recently in the Tarim Basin, NW China. Constraining the evolutionary history of fluid activity and the process of oil charging in the deeply buried carbonate reservoirs is crucial for further hydrocarbon exploration and development. In this study, we conducted an integrated study combining petrography, geochemistry, fluid inclusion analysis, and in-situ U–Pb dating of multi-phase calcite veins collected from the Ordovician carbonate reservoirs, Akeyasu area, Tarim Basin. Four distinct phases of calcite veins were found within the Ordovician carbonate reservoirs. The U–Pb ages of these four phases of calcite veins are as follows: 459.8 ± 4.7 Ma, 418.8 ± 3.7 Ma, 329.7 ± 1.6 Ma to 310.0 ± 3.6 Ma, and 291.7 ± 6.1 Ma to 220.5 ± 7.3 Ma. These ages closely correspond to key tectonic events that influenced the study area, specifically, the Middle Caledonian, Late Caledonian, Middle Hercynian, and Late Hercynian orogenies, respectively. The first phase (C1) calcite veins are believed to have precipitated from meteoric water, closely associated with the first episode of oil charging. Phase Ⅱ (C2) calcite veins primarily formed from Early-Middle Ordovician formation water. Phase Ⅲ and phase Ⅳ (C3 and C4) calcite veins originated from Middle-Late Cambrian formation water and hydrothermal fluids, respectively. Significantly, the second and third episodes of oil charging occurred concurrently with the precipitation of C3 and C4 calcites. Seismic data analysis of Paleozoic strata in the Akeyasu area revealed four stages of fault activity, corresponding to the Early Caledonian, Middle Caledonian, Late Caledonian-Early Hercynian, and Middle-Late Hercynian orogenies. Our findings strongly suggest that the evolution of fluid activity and oil charging was primarily governed by regional orogeny. These large-scale tectonic shifts led to intensified fault activity, ultimately controlling fluid activity and oil charging dynamics. Furthermore, this intensified faulting eased the connection between deep fluid systems and the Ordovician carbonate reservoirs, serving as the primary conduits for fluid migration and oil charging within the deeply buried Ordovician carbonate reservoirs.
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