Microbiological Preparations Research Articles

The Influence of Bisolbifit as a Fertilizer Modifier on the Oat Productivity

The purpose of the research was to study the effect of mineral fertilizers and biological product Bisolbi-fit as a modifier on the oat productivity. The studies were carried out on the experimental field of Ulyanovsk Research Institute of Agriculture, branch of Sam SC RAS. The soil was leached, heavy-clayey chernozem. The experiment was carried out in a 3-fold repetition by the method of split plots with a systematic arrangement of options. The total area of one plot was 145 m2 (5.8×25), the accounting area was 100 m2 (4×25). The experiment scheme was as follows: 1. Control; 2. Presowing seed treatment with BisolbiFit in a dose of 400–600 g/t; 3. N15P15K15 in a dose of 15 kg ai/ha; 4. N15P15K15m in a dose of 15 kg ai/ha; 5.½ N15P15K15m in a dose of 7.5 kg a.i./ha. The effectiveness of the fertilizers was assessed during the cultivation of crops for three backgrounds: 1) zero – without fertilization; 2) NH4NO3m – treated with BisolbiFit in the dose of 40 kg a.i./ha; 3) NH4NO3 – in a dose of 40 kg ai/ha. It was established that the use of the microbiological preparation based on the Bacillus subtilis strain Ch-13, intended both for inoculation of seeds and use as a biomodifier of mineral fertilizers, had a positive effect on the crop structure, yield and grain quality. The most effective option was the one with Azofoska applied in a dose of 15 kg and modified with BisolbiFit at the rate of 4 kg per one ton of fertilizers on all the backgrounds. The use of BisolbiFit both for the pre-sowing seed treatment and treatment of mineral granules, increased the NPK payback by the yield gain.

Модификация метода искусственного заражения проростков подсолнечника возбудителем фомоза в лабораторных условиях для определения колонизирующей активности штаммовпродуцентов микробиопрепаратов

We carried the work in the biomethod laboratory of the crop management department of V.S. Pustovoit All-Russian Research Institute of Oil Crops. We modified the method of artificial inoculation of sunflower seedlings with a pathogen in a laboratory conditions for the secondary screening of antagonist strains from the collection of the biomethod laboratory to Phoma rot pathogen. We developed a five-point scale for evaluation of affection degree of sunflower seedlings by Phoma rot pathogen: 0 points – healthy seedlings; 1 point – darkening of the root tip, intensive development of lateral roots; 2 points – darkening of the root by a third or up to the middle, but intensive development of lateral roots; 3 points – necking of rot in the middle of the root or between the hypocotyl and the root, lateral roots are poorly developed; 4 points – root rotting to the middle or necking between the hypocotyl and the root, but intensive development of lateral roots; 5 points – complete rotting of the root, lateral roots are poorly developed or absent; 1-3 – viable seedlings; 4-5 – non-viable seedlings. The optimal period of exposure of the root tips of sunflower seedlings to the pathogen colony equal to 3 hours develops an average background of infection with the pathogen (60.0 %) already on the first day, at which it is possible to evaluate at an early stage the difference between the variants by the colonizing activity of laboratory samples of microbiological preparations.

Transformation of oil and hexadecane in soil by microbial preparations and earthworms

This study focuses on the effect of earthworms, oil-decomposing microbial preparation Destroil, and their combination on the destruction of oil in contaminated soil. The earthworms Eisenia andrei Bouché, 1972 accelerated the transformation of oil and hexadecane by microbiological preparations Destroil and Devouroil. The use of the combination of biological product and earthworms resulted in: (1) maximum reduction in the content of oil and hexadecane in the soil samples under study; (2) reduction of the phytotoxicity of oil-contaminated soil; (3) improvement in the agrophysical properties of soils in comparison with the action of these biodegradants separately. The transformation of oil hydrocarbons was monitored using NMR and IR spectroscopy. The biological oxidation process was accompanied by the accumulation of unsaturated triglycerides of variable structure. The chain length of the acid residue of triglycerides varied within the C11-C22 carbon atoms. In this case, the number of carbon atoms in the chain was most often -C18. The position of the double bonds was stochastic. At the same time, they were all removed from the carboxyl group by more than 4 carbon atoms. Stimulation of the processes of complex biotransformation of hexadecane under the action of the drug Devouroil and earthworms was manifested with the introduction of a biological product at concentrations of 32 × 1010 cells/kg and 64 × 1010 cells/kg. A further increase in the number of cells of the biological product Devouroil with the combined use of the biological product and worms led to a leveling of the stimulating effect of earthworms on the transformation processes of this hydrocarbon. At the same time, the phytotoxicity of the studied samples decreased.

Study of enzymatic activity of microbiological biopreparation in the cultivation of cotton plant (Gossypium hirsutum L.) under saline stress conditions

The changes in enzymic activity of the soils have been studied in the result of inoculation of cotton plants with microbiological preparation “Zamin-M” which was obtained on the base of association of strains Pseudomonas stutzeri SKB-308, Bacillus subtilis SKB-309 and Bacillus megaterium SKB-310 isolated from the rhizosphere of cotton plant grown in different saline soils. The results of the study showed that when the biopreparation “Zamin-M” was used, the enzymic activity of peroxidase was 1.76 ± 0.32 and in the control 1.5 ± 0.31 (mg purpurgalin/g soil, 24 hours), as well as the enzymic activity of polyphenol oxidase was 2.1 ± 0.53 and in the control 1.64 ± 0.51 (mg purpurgalin/g soil, 24 h). In the control variants, the activity of the enzyme of catalase relative to 1 g of absolutely dry soil was 1,0 ± 0,55 according to the amount of oxygen released in 5 minutes, while using the preparation its increase was observed and made 1.8±0.27 ml O2.Similar indicators were found in the study of invertase enzymic activity, when the preparation “Zamin-M” was applied, its amount constituted 271 ± 0.55 (mkg/absolutely dry soil), while in the control variant it made 111 ± 0.41 (mkg/absolutely dry soil). Urease enzymic activity was active under 24 ± 0.3 units when the preparation was used and in the control it was noted to be 14 ± 0,64 (mkg N/NH4/1 g soil) and phosphatase enzymic activity made 74±0.21 and in the control 53±0.32 (mkg P2O5/g soil, 24 hr) which led to the activation of biochemical processes in the soil.

The effect of azotovit and phosphatovit on the yield of vetch oats

In the Novgorod region, the effectiveness of the integrated use of new microbiological preparations of Azotovit, Phosphatovit and mineral fertilizers has been investigated. The purpose of the experiment was to increase the yield of green mass of oatmeal mixture. Field experiment took place in 2017-2019 on sod-podzolic light loamy, clay-laden soil. Of the varieties recommended for cultivation in the North-West region, the following objects of research were used: spring vetch Lyudmila and oats of spring variety Borrus. Three methods of applying micronutrient fertilizers were tested: presowing treatment of seeds; foliar treatment in the phase of branching the wiki and tillering of oats; seed treatment presowing + foliar in the phase of branching the vetch and tillering of oats on two backgrounds of mineral nutrition - background 1 – N82P105К70 and background 2 – N41P53К35. The highest yield of green mass of oatmeal mixture (on average over 3 years) – 42.4 t/ha (6.8 t. units/ha) and digestible protein of 0.85 t/ha were obtained with a dose of mineral fertilizers per calculated yield N82P105K70 during seed treatment before sowing with microbiological fertilizers (Azotovit, Phosphotovit), as well as spraying the ground vegetative part of the vetch (in the branching phase) and oats (in the tillering phase). Doses: Azotovit –1.0 l/ha + Phosphotovit – 1.0 l/ha. At the same time, the energy intensity of production of one ton of feed units in this embodiment was 2.2 GJ. In addition, energy efficiency was 4.7 units, and the increase in soil energy potential was 15 GJ/ha.

Complex composts influence on the growth and dynamics of vermiculture number

The paper examines the influence of complex composts from waste water residues and brewers grains on the growth dynamics of Eisenia foetida vermiculture in greenhouse conditions as a regional aspect of sustainable development of the Ugra territory. The substrates were used: soil with a total humus content of 1,83 0,51%, waste water residues, brewers pellet, plants, food waste, paper, bird droppings, microbiological additive Tamir, as well as 50 immature worms Prospector. The experience lasted 30 days. Each compost variant contained 7 replications with microbiological additive Tamir and worms, as well as 2 controls without worms: with and without microbiological additive Tamir. Composite composts showed a positive trend in the increase in the number of earthworms and their total weight. A decrease in the mass of the substrate after vermicomposting using the microbiological preparation Tamir was observed in all variants. A statistically regular decrease in the mass of the substrate with time was established due to the growth of worms. The optimum temperature for the development of worms is +21C, and the pH values at which a high number of worms were observed are in the range from 7,8 to 8,2. Thus, all types of complex composts can be used. The process of decomposition and positive dynamics of growth of worms was observed everywhere at different rates. The use of brewers grains in a raw state is possible with the introduction of 1025% of the total mass of the compostable substrate with organic components having a sorption capacity.

Применение новых комплексных удобрений и биологических препаратов на посевах кормовой озимой ржи в условиях Псковской области

Abstract. The development of optimal nutritional system for agricultural crops is one of the main factors for the getting of stable yields. The deficiency of some macro- and microelements in plant nutrition, leading to yield decreasing, can be compensated with new complex of balanced fertilizers and biological products. The aim of the work is to study the effect of nitrogen fertilization, new forms of complex fertilizers based on active biological substances (“Kodamin B-Mo”, “K-Gumat-Na”, “Agroflorin” “Auksinolen”) and the microbiological preparation (MBP) “Bisolbifit” on the yield and quality of grain of fodder winter rye “Novaya Era” on sod-podzolic soils in the Pskov region. The originality is that for the agro-ecological conditions of the Pskov region, the influence of new complex fertilizers, biological and humic preparations in the cultivation of winter rye has been studied. Methods. The research was carried out in 2019 on the experimental field of the laboratory of agricultural technologies of the Federal research center for Bast Crops (Pskov Department). The records, observations and analyzes were carried out according to generally accepted methods. Results. The maximal positive effect on grain yield was made with the introduction of complete mineral fertilizer in doses of N80P40K70 with fractional nitrogen application: 20 kg of active substance – in the main fertilizer, 40 kg – in the tillering phase and 20 kg – in the booting phase. Up to 1,3 t/ha or 42.2 % to the control level were obtained in that variation. Application of mineral fertilizers in a dose of N40P40K70 and MBP “Bisolbifit” increased on 0,4 t/ha of grain, which amounted 10.6 %. Foliar double treatment of plants with fertilizer “Kodamin B-Mo” increased grain yield up to 8.6 %. The influence of the investigated factors on the protein content and nitrate nitrogen in plants of fodder winter rye, the weight of 1000 seeds and the structure of the yield was established. For 1 kg of active substance of mineral fertilizers at various doses of nitrogen fertilizing the additional yield of grain from 3.68 to 6.39 kg was obtained.

Efficiency Of Helitec Against Cotton Scoop (Heliothis Armigera Hb) On Beans

The article presents the results of testing the microbiological preparation HELITEC against cotton bollworm on beans. The results of the experiment showed that in the experimental variants against the first generation, in two farms treated with Helitec at a rate of 0.300-0.400 l / ha with a liquid consumption of 300 l / ha, the biological efficiency was, respectively, on the 14th day after treatment, 96.0-93, 0%. The advantages of the microbiological method are both the possibility of prevention and their safety for humans, warm-blooded animals and the environment.

Шляхи розвитку промисловості з виробництва біологічних засобів захисту рослин в Україні

The problem of industrial production of biological plant protection products in Ukraine is important and relevant. The purpose of this study is to determine the principles of innovative approaches in the production of plant protection products based on microbiological drugs and entomophages and to propose the organizational structure of production of these products in Ukraine. Methods of theoretical generalization, research in applied microbiology and entomology, analysis and synthesis were used. The basic technological and technical methods and equipment for production at enterprises of different levels of biological plant protection products (BZZR) and their application in agrocenoses developed by ITI "Biotechnics" of NAAS are resulted, the general structure of system of biological protection of plants in Ukraine is offered. Emphasis is placed on specific technologies and equipment for the production of microbiological preparations of various scales: bacterial, fungal, viral, the use of nematodes. Attention is paid to the production of entomological drugs to protect plants from pests. Ground methods of BZZR introduction into agrocenoses and with the use of aviation are offered. The proposed innovative approaches to the development, creation and use of OSH in Ukraine, based on the system of bioproduction of these tools, will significantly increase their use in plant protection in organic production and the share of integrated plant protection. The ways of development, creation of industrial production and use of biological plant protection products in Ukraine are considered and offered, based on the developed system of bioproductions of these means. This will significantly increase their use in plant protection in organic production and the share of integrated plant protection.

Substantiation of work quality indicators of the universal seeds scarificator of the eastern galega (<em>Galega Orientalis</em>)

Eastern galega (Galega orientalis) contains amino acids and vitamins necessary for the normal functioning of the animal organism. In addition, it is a good precursor to many cultures. One of the drawbacks of Eastern galega is the hard shell of the seeds, which leads to increased seed consumption, a prolonged time of seedlings and an irrevocable loss of part of the sown seeds. The most affordable, simple and productive is the mechanical method of violating the tightness of the coating film of seeds. The aim of the study was to conduct comparative experiments with the existing scarifier, to create an improved design of a disk scarifier, which allows to improve the quality of seed treatment, and the theoretical justification of its structural and technological parameters. The original design of the disk scarifier on the seeds of the Eastern galega was investigated. The working bodies of the blades of the scarifier disc and their changing installation angle allows to adjust the contact angle of the seeds with the abrasive surface of the grinding wheel, thereby scratching the hard shell of the seeds without injuring them. In contrast to the used designs of scarifiers, the device under development provides for a pneumatic system for removing dust and impurities from the abrasive surface of the grinding wheel by the air flow and additional seed treatment with the microbiological preparation rhisotorfin. In the study of qualitative indicators of scarification of Eastern galega at an average moisture content of 9%, the following parameters were determined: seed germination,%; the degree of scarification,% and the degree of crushing of seeds,% with disk rotation modes - 700, 900, 1100, 1300, 1500 rpm. The studies have shown high-quality seed treatment compared to its counterpart. In the recommended range of disk revolutions of 900 ... 1100, the proposed scarifier showed a decrease in the degree of crushing of seeds by 0.2 ... 0.4%, an increase in the degree of scarification by 2 ... 3% and the degree of germination by 4 ... 5%. The use of the developed design of the scarifier in international practice will reduce the damage of the treated seeds and increase the yield of legumes.

Аналіз ризосферного мікробіому цукрової кукурудзи за впливу хімічних і біологічних протруйників

У статті представлено результати впливу хімічних і біологічних протруйників на кількісний склад мікробіому ризосфери цукрової кукурудзи протягом її вегетації. Показано, що за використання хімічних препаратів у ризосфері рослин на порядок знижується кількість амоніфікаторів та мікроскопічних грибів у першій половині вегетаційного періоду культури. Вплив ксенобіотиків на вміст актиноміцетів, олігонітрофілів та педоторофів є менш токсичним. У порівнянні з контрольним варіантом вміст даних мікроорганізмів знижувався на 50-75 %, при цьому змін зазнає не лише кількісний, а й якісний склад ризосферної мікрофлори. У той же час досліджувані пестициди не впливали на вміст актиноміцетів і оліготрофів. По мірі розвитку рослин негативний вплив хімічних протруйників на мікрофлору ґрунту поступово нівелювався. Відбувалося відновлення складу ризосферної мікробіоти у кожній умовній функціональній групі мікроорганізмів і до завершення вегетації кукурудзи мікробний ценоз повертався до свого первинного кількісного і якісного складу. Застосування мікробіологічних засобів захисту не призводило до змін чисельності чи дисбалансу у складі ґрунтової мікробіоти. Її кількісний та якісний склад відповідав контрольному варіанту.

Sterility of gamma-irradiated pathogens: a new mathematical formula to calculate sterilizing doses.

In recent years there has been increasing advocacy for highly immunogenic gamma-irradiated vaccines, several of which are currently in clinical or pre-clinical trials. Importantly, various methods of mathematical modelling and sterility testing are employed to ensure sterility. However, these methods are designed for materials with a low bioburden, such as food and pharmaceuticals. Consequently, current methods may not be reliable or applicable to estimate the irradiation dose required to sterilize microbiological preparations for vaccine purposes, where bioburden is deliberately high. In this study we investigated the applicability of current methods to calculate the sterilizing doses for different microbes. We generated inactivation curves that demonstrate single-hit and multiple-hit kinetics under different irradiation temperatures for high-titre preparations of pathogens with different genomic structures. Our data demonstrate that inactivation of viruses such as Influenza A virus, Zika virus, Semliki Forest virus and Newcastle Disease virus show single-hit kinetics following exposure to gamma-irradiation. In contrast, rotavirus inactivation shows multiple-hit kinetics and the sterilizing dose could not be calculated using current mathematical methods. Similarly, Streptococcus pneumoniae demonstrates multiple-hit kinetics. These variations in killing curves reveal an important gap in current mathematical formulae to determine sterility assurance levels. Here we propose a simple method to calculate the irradiation dose required for a single log10 reduction in bioburden (D10) value and sterilizing doses, incorporating both single- and multiple-hit kinetics, and taking into account the possible existence of a resistance shoulder for some pathogens following exposure to gamma-irradiation.

The effect of complex composts on the growth and dynamics of the number of vermiculture Esenia Fetida

The article considers the complex composts role from the wastewater residues and beer pellets on the Esenia fetida vermiculture growth dynamics in closed ground conditions as the Yugra territory sustainable development regional aspect. The substrates used: the soil with a total humus content of 1.83 ± 0.51%, the wastewater residues, the brewer pellet, the plants, the food waste, the paper, the bird droppings, the Tamir microbiological preparation, as well as 50 immature worms, the hybrid Prospector. The experiment lasted 30 days. In each compost variant, there were 7 replicates with the MP and worms, as well as 2 controls without worms: with MP and without it. The complex composts showed a positive trend in the increase in the earthworms number and their total mass. The decrease in the substrate mass after vermicomposting using the Tamir microbiological preparation was observed in all variants. A statistically regular decrease in the substrate mass over time due to the growth of the worms was found. The optimum temperature for the worm’s development is +21 degrees, and the pH values at which a high worms number were observed are in the range from 7.8 to 8.2. Thus, it is possible to use all the complex composts variants. The decomposition process and the growth of the worms positive dynamics were observed everywhere at different rates. The beer pellets use in the raw state is possible when adding 10-25% of the compostable substrate total mass with the organic components that have sorption capacity.

Influence of microbiological preparations on spring wheat yield

Based on the research carried out on light-gray forest soils of the Chuvash Republic, elements of spring wheat cultivation technology were developed and recommended for production, ensuring the formation of a t/ha yield with an increase of t / ha in relation to control. Evidence-based data on more effective use of microbiological preparations were obtained.

The Use of Air Induction Nozzles for Application of Fertilizing Preparations Containing Beneficial Microorganisms

The work presents the structure and characteristics of field sprayer nozzles, as well as their impact on the survival of beneficial organisms in the selected fertilizing preparations. EŻK and EŻKT nozzles, (EŻK and EŻKT are trade names of single and twin jet air induction nozzles, respectively), that are available on the market have shown low efficiency in the discussed characteristics. Survival of microorganisms under initial conditions at 13.6 × 106 cfu/mL and pressure of 0 MPa, under critical conditions dropped to 1.7 × 106 cfu/mL for EŻK02 and 1.2 × 106 cfu/mL for EŻKT02, in both variants at a pressure of 0.5 MPa. When increasing the flow rate of the components, i.e., the size of the outlet orifices, it was observed that the survival of microorganisms increased by about 11.3% compared to the previously tested component. This resulted from the negative impact of the following: the pressure generated by the application device, number of outlet orifices, and size of an outlet orifice. The results of survival of microorganisms are given in the colony-forming unit (CFU). In addition to providing guidelines useful in the creation of a prototype sprayer intended for use in the application of microbiological preparations, the presented characteristics are a source of information for the end user as regards the proper conditions for the application of these preparations.

Culturable endophytic bacteria from stems and leaves of garden pea (Pisum sativum L.)

Background. Endophytic microorganisms inhabit internal tissues of most plants. However, little is known about endophytic community of the garden pea (Pisum sativum L.), an agriculturally important crop.
 Materials and methods. Culturable endophytic bacteria were isolated from sterilized stems and leaves of three pea genotypes: K-8274 (cv. Vendevil), K-3358 (unnamed cultivar), and cv. Triumph. The taxonomic position of isolates was determined by 16S rRNA gene sequencing. The plant growth-promoting capabilityof identified bacteria was tested on the roots of watercress (Lepidium sativum L.).
 Results. In total, out of 118 morphotypes of culturable endophytic bacteria identified, for 80 the taxonomic position was determined. Proteobacteria and Firmicutes were dominant phyla, and Actinobacteria were present in minority. Eight bacterial isolates demonstrated the plant growth-promoting capability, and one of them KV17 (Rahnella sp.) maintained this capability after several passages and prolonged storage.
 Conclusion. The plant growth-promoting bacteria isolated from pea stems and leaves can become a component of microbiological preparations.

NFLUENCE OF PREPARATION VETOM 3 ON GROWTH AND DEVELOPMENT OF CARP LARVAE (<i>CYPRINUS CARPIO</i> L.) GROWING AND CONDITIONS OF INSTALLATION CLOSED WATER SUPPLYEA

Researches of the microbiological preparation Vetom 3 made on the basis of Bacillus amyloliquefaciens in NPF «Research center» LLC, on the larvae of the Altai mirror carp received in the conditions of factory reproduction in the research center of aquaculture of NSAU are carried out. To achieve this goal, 2 experimental groups and 1 control group were formed from the larvae of the Altai mirror carp. In a production experiment investigated the effect of different doses 200 mg/kg diet and 300 mg/kg diet and patterns of use the preparation on the safety of the larvae, the absolute mass, relative and average daily gain. Immediately before use, the preparation was diluted in water and mixed with feed. The duration of the preparation was 12 days. The water temperature during the experiment period was in the range of 18,8–20,9 ˚С. All groups were kept in similar conditions. It was found that the probiotic preparation Vetom 3 contributes to the safety, average daily and relative growth of larvae the Altai mirror carp; the maximum safety data amounted to 97,7 %, absolute weight – 156,15 mg; average daily and relative growth – 10,99 mg and 6,07 %. These results were obtained when using Vetom 3 at a dose of 300 mg / kg of feed daily 1 time per day for 5 days, then a day later for another 7 days before the end of the experiment.

Компостування птиці, що загинула

From 3 to 10% of the poultry in the process of growing and keeping dies from one reason or another (illness, cannibalism in the herd, etc.), and the disposal of such a bird is now a big problem for many poultry farms. Current practice involves its burial in the so-called “Czech pits”, burning or processing in autoclaves, which requires significant costs and can lead to environmental pollution. Composting such a bird is much cheaper, but it also raises the concerns of some specialists regarding the possible negative impact on the environment. The purpose of these studies was to study the kinetic laws of the composting process of dead birds, its impact on the environment, the quality and safety of the compost obtained. An experiment was conducted in which the corpses of birds were composted together with litter droppings in a ratio of 1: 6 in two versions: without treatment with a microbiological preparation (D1) and a treated microbiological preparation (D2). Option (K) was used as a control, in which the litter was composted without the addition of poultry corpses and treatment with a microbiological preparation. It was found that in the process of composting, the temperature of the raw material reached 64-66 °C, the duration of the period with temperatures of more than 60 °C was 5-9 days, which according to previous studies is enough to neutralize most types of pathogenic microorganisms. Processing the raw material with a microbiological preparation contributed to an increase in the temperature of the substrate in individual periods of the active composting phase by 1-5 °C and a decrease in ammonia emission by 1.1-1.2 times. In both cases (D1 and D2), almost complete decomposition of the corpses of birds was ensured for 45 days. Composting the litter together with the dead bird allowed to increase the nitrogen content in the finished compost. In all cases, bacteria of the genus E. coli and Salmonella were not found in the finished compost.

Характеристика эффективности и токсичности современных инсектоакарицидов, ларвицидов, регуляторов развития насекомых и репеллентов. Часть 4. Регуляторы развития насекомых, микробиологические препараты, репелленты

Характеристика эффективности и токсичности современных инсектоакарицидов, ларвицидов, регуляторов развития насекомых и репеллентов. Часть 4. Регуляторы развития насекомых, микробиологические препараты, репелленты

The influence of biostimulants and microbiological preparation on the production process of peas (Pisum sativum L.) in the drought Steppe of Ukraine

The use of plant growth bioregulators and microbiological preparations stimulates growth processes, improves nitrogen nutrition, and enhances crop resistance to adverse conditions. Therefore, the aim of the work was to elucidate the characteristics of the additive effect of biostimulants (Stimpo, Regoplant) and the microbiological preparation Azotofit-R on the growth, development, formation of a photoassimilating apparatus and the productivity of peas Oplot variety in the dry Steppe of Ukraine. Presowing and foliar treatments with biological products were made in the doses recommended by the manufacturers. The number of root nodules was calculated, the leaf area index, the content of chlorophyll were determined, and the netto-photosynthesis productivity was calculated in the experiment. The structural elements of the biological productivity of pea crops were recorded. It is established that Stimpo and Regoplant combined with Azotofit-r contributed to increase in the numbers of nodules by 14–17 % till flowering stage in comparison with the variants under the separate application of biostimulants. Presowing treatment of peas seeds and foliar treatment with more active leaves surface formation was noticed under combined application of biopreparations compared to separate one and it promoted the raise of netto-photosynthesis. Synergistic interaction of biostimulants (Stimpo, Regoplant) with bioactivator (Azotofit) was observed in the processes of chlorophyll synthesis and accumulation. Combined interaction of Stimpo, Regoplant with Azotofit rised the beans number per plant by 8 % to 28 %, and weight of 1000 seeds by 4.4 % to 6.3 % respectively. Under separate application of Azotofit, Stimpo, Regoplant, the biological yield of peas crop was 3.4, 3.8 and 3.4 t/ha. Whereas, under combined application of biopreparations (Azotofit+Stimpo) the yield was – 4.4 t/ha and (Azotofit+Regoplant) – 4.2 t/ha and its exceeded the control yield (3.1 t/ha) of peas. These data confirm the results of biopreparations positive influence on legumine biological productivity, which indicates a future perspective to research the productive processes under biopreparations effect. Key words: biostimulator, Rehoplant, Stimpo, Azotofit, pea, photoassimilation apparatus, yield.