Microbial Flora Research Articles

The Application of Photobiomodulation and Stem Cells Seeded on the Scaffold Accelerates the Wound Healing Process in Mice.

Introduction: The purpose of this research was to test the impact of seeding a hydrogel chitosan scaffold (HCS) with human adipose-derived stem cells (hADSCs) under the influence of photobiomodulation (PBM) on the remodeling step on the wound repairing process in mice. Methods: Thirty mice were randomly assigned to five groups (n=6 per group ): The control group (group 1) consisted of mice without any intervention. In group 2, an HCS was implanted into the wound. In group 3, a combination of HCS+hADSC was inserted into the wound. In group 4, an HCS was inserted into the wound and PBM was applied. In group 5, a combination of HCS+hADSCs was inserted into the wound, followed by PBM treatment. Results: Improvements in the injury closing rate (WCR) and microbial flora were observed in all groups. However, the highest WCRs were observed in group s 5, 4, 3, and 2 (all P values were 0.000). Groups 3-5 showed increased wound strength compared to group s 1 and 2, with group 2 demonstrating better results than group 1 (P values ranged from 0.000 to 0.013). Although group s 3-5 showed increases in certain stereological elements compared to group s 1 and 2, group 2 exhibited superior results in comparison with group 1 (P values ranged from 0.000 to 0.049). Conclusion: The joined use of HCS+hADSCs+PBM significantly accelerated the wound healing process during the maturation phase in healthy mice. This approach demonstrated superior wound healing compared to the use of HCS alone, hADSCs+HCS, or PBM+HCS. The findings suggest an additive effect when HCS+hADSCs+PBM are combined.

The role of IL-37 in gastrointestinal diseases.

Gastrointestinal mucosal surface is frequently under challenge due to it's the large surface area and most common entry of microbes. IL-37, an anti-inflammatory cytokine, regulates local and systemic host immunity. H. pylori infection leads to the inhibition of IL-37 in the gastric mucosa, contributing to heightened mucosal inflammation and destruction, thereby facilitating increased proliferation of H. pylori. Food allergy, due to immune dysregulation, also contribute to GI injury. On the other hand, elevated levels of IL-37 observed in gastric cancer patients align with reduced host immunity at the cellular and humoral levels, indicating that IL-37 may contribute to the development of gastric cancer via suppressing pro-inflammatory responses. While IL-37 provides protection in an IBD animal model, the detection of highly produced IL-37 in IBD patients suggests a stage-dependent role, being protective in acute inflammation but potentially exacerbates the development of IBD in chronic conditions. Moreover, elevated colonic IL-37 in CRC correlates with overall survival time and disease time, indicating a protective role for IL-37 in CRC. The differential regulation and expression of IL-37 between upper- and lower-GI organs may be attributed to variations in the microbial flora. This information suggests that IL-37 could be a potential therapeutic agent, depending on the stage and location.

Experimental Models in Unraveling the Biological Mechanisms of Mushroom-Derived Bioactives against Aging- and Lifestyle-Related Diseases: A Review.

Mushrooms have garnered considerable interest among researchers due to their immense nutritional and therapeutic properties. The presence of biologically active primary and secondary metabolites, which includes several micronutrients, including vitamins, essential minerals, and other dietary fibers, makes them an excellent functional food. Moreover, the dietary inclusion of mushrooms has been reported to reduce the incidence of aging- and lifestyle-related diseases, such as cancer, obesity, and stroke, as well as to provide overall health benefits by promoting immunomodulation, antioxidant activity, and enhancement of gut microbial flora. The multifunctional activities of several mushroom extracts have been evaluated by both in vitro and in vivo studies using cell lines along with invertebrate and vertebrate model systems to address human diseases and disorders at functional and molecular levels. Although each model has its own strengths as well as lacunas, various studies have generated a plethora of data regarding the regulating players that are modulated in order to provide various protective activities; hence, this review intends to compile and provide an overview of the plausible mechanism of action of mushroom-derived bioactives, which will be helpful in future medicinal explorations.

Isolation, Characterization and Evaluation of Pseudomonas aeruginosa in the Biodegradation of Propanil Contaminated Rice Farms

Propanil are herbicide commonly used on rice farm for the control of weeds and pests. Like other herbicides, tendencies to persists in the soil and impact on the microbial flora of rice farm soils does exists although study finds that propanil is not recalcitrant. This work aimed to isolate, characterize, and evaluate Pseudomonas aeruginosa from Enugu propanil contaminated rice farm. A total of nine [9], contaminated soil samples {three each from Nenwe, Ugbawka and Akpuoga-Nike} were collected and analyzed for presence of P.aeruginosa. The average total count were 3.8x106 [Ugbawka], 3.5x106 (Nenwe) and 2.8x106 (Akpuoga-Nike). Out of nine samples collected, 7 samples were positive for P.aeruginosa. Nine soil samples yielded 13 Pseudomonas aeruginosa isolates. Out of 13 P.aeruginosa isolated 4(30.8%) from Nenwe, 6(46.1%) from Ugbawka and 3(23.1%) from Akpuoga –Nike soil samples. P.aeruginosa were more in Ugbawka followed by Nenwe. The ability to utilize propanil was tested with other isolates based on their growth. It was observed that Pseudomonas aeruginosa and mixed culture showed heavy growth in mineral salt propanil media than in the media without propanil. The results of molecular identification revealed that Pseudomonas aeruginosa from two different strains utilized propanil heavily than other isolates. The result of the varying concentration of propanil showed that Pseudomonas aeruginosa (S1) had increased growth with increase in concentration of propanil from 0.8 at 1ml to 1.2 at 2ml. S2 showed 0.3 at 1ml to 1.0 at 2ml and mixed culture showed 0.6 at 1ml to 0.9 at 2ml. The result showed that P.aeruginosa is an efficient propanil degrader. Application of P.aeruginosa through fertilizer or soil amendment could be used to manage propanil pollution in the agricultural system.

Oral Prevalence of Selenomonas noxia Differs among Orthodontic Patients Compared to Non-Orthodontic Controls: A Retrospective Biorepository Analysis.

The oral microbial flora may be significantly altered by orthodontic therapy and the use of fixed orthodontic brackets. Most orthodontic research has focused on cariogenic pathogens, while some evidence has demonstrated an increase in many known periodontal pathogens. However, little is known about the prevalence of the Gram-negative periodontal pathogen Selenomonas noxia (SN) among these patients. Using an existing saliva biorepository, n = 208 samples from adult and pediatric orthodontic and non-orthodontic patients were identified and screened for the presence of SN using qPCR and validated primers. In the pediatric study sample (n = 89), 36% tested positive for the presence of SN, with orthodontic patients comprising more SN-positive samples (87.5%) than SN-negative samples (78.9%), p = 0.0271. In the adult study sample (n = 119), SN was found in 28.6%, with orthodontic patients comprising 58.8% of positive samples and only 28.2% of negative samples (p < 0.0001). These data demonstrated that both pediatric and adult orthodontic patients exhibited higher prevalence of SN compared with age-matched non-orthodontic controls. As this microorganism is associated not only with periodontal disease but also long-term health issues such as obesity, more research is needed regarding the factors that increase the prevalence of this microbe.

Oral microbiota diversity in moderate to severe plaque psoriasis, nail psoriasis and psoriatic arthritis

Gaining a comprehensive understanding of the role played by the oral microbiome in moderate to severe plaque psoriasis and its potential implications for disease management and development holds significant importance. With the objective of exploring correlations between the oral microbiota and severe psoriasis, this study involved 72 severe psoriasis patients and 16 healthy individuals, whose clinical manifestations and living habits were carefully recorded. Cutting-edge techniques such as 16S rRNA gene sequencing and bioinformatics analysis were employed to compare the microbial flora, investigating dynamic changes among severe plaque psoriasis patients, psoriatic arthritis patients and healthy individuals. The findings revealed noteworthy patterns including increased levels of Aggregatibacter in the psoriatic arthritis group, accompanied by a decrease in the level of Prevotella. Moreover, the enrichment o Capnocytandophaga (P = 0.009), Campylobacter (P = 0.0022), and Acetobacter (P = 0.0292) was notably more substantial in the psoriasis group compared to the control group, whereas certain bacterial species such as Bacteroides (P = 0.0049), Muribaculaceae (P = 0.0048) demonstrated decreased enrichment. Additionally, the psoriatic arthritis group exhibited significantly higher levels of Ralstonia, Bifidobacterium and Micromonospora. Based on these findings, it can be inferred that individuals with lower levels of Prevotella and higher levels of Corynebacterium may be more susceptible to psoriasis exacerbation.

Clostridium butyricum Prevents Diarrhea Incidence in Weaned Piglets Induced by Escherichia coli K88 through Rectal Bacteria-Host Metabolic Cross-Talk.

This study aimed to evaluate the effects of Clostridium butyricum (C. butyricum) on the prevention of the diarrhea rates and growth performances of weaned piglets induced by Escherichia coli K88 (E. coli K88). Twenty-four weaned piglets (6.92 ± 0.11 kg) were randomly assigned to one of three treatment groups for a period of 21 days. Each group consisted of eight pigs, with each pig being housed in an individual pen. Group I received the control diet along with normal saline, Group II received the control diet along with E. coli K88, and Group III received the control diet supplemented with 5 × 108 CFU/kg of C. butyricum and E. coli K88. We examined alterations in rectal microbiota and metabolites, analyzed the incidence of diarrhea, and investigated the interactions between microbiota and metabolites through the application of Illumina MiSeq sequencing and liquid chromatography-mass spectrometry. The results showed that, from days 14 to 21, the diarrhea incidence in Group III decreased significantly by 83.29% compared to Group II (p < 0.05). Over the entire experimental duration, the average daily feed intake of Group III decreased significantly by 11.13% compared to Group I (p < 0.05), while the diarrhea incidence in Group III decreased by 71.46% compared to Group II (p < 0.05). The predominant microbial flora in the rectum consisted of Firmicutes (57.32%), Bacteroidetes (41.03%), and Proteobacteria (0.66%). Administering E. coli K88 orally can elevate the relative abundance of Megasphaera (p < 0.05). Conversely, the supplementation of C. butyricum in the diet reduced the relative abundance of Megasphaera (p < 0.05), while increasing the relative abundance of unclassified_f_Lachnospiraceae (p < 0.05). Rectal metabolomics analysis revealed that supplementing C. butyricum in the feed significantly altered the amino acids and fatty acids of the piglets infected with E. coli K88 (p < 0.05). The correlation analysis showed that the occurrence of diarrhea was inversely related to adipic acid (p < 0.05) and positively associated with (5-hydroxyindol-3-YL) acetic acid and L-aspartic acid (p < 0.05). Prevotella_1 exhibited a negative correlation with octadecanoic acid (p < 0.05). Prevotellaceae_UCG-005 showed a negative correlation with (5-hydroxyindol-3-YL) acetic acid (p < 0.05). The findings from this research study aid in probiotic development and the enhancement of healthy growth in weaned piglets.

Abstract Tu022: Gut-derived bacterial metabolites promote cardiac inflammation, leading to heart failure in murine model of DSS-induced colitis

Background: Inflammatory Bowel Disease (IBD) affects millions of people all over the world. This has been known to contribute to several other comorbidities. Recently, impaired gut-barrier permeability has been known to contribute to cardiovascular diseases in IBD patients. However, the precise mechanisms remain unclear. In this study, we propose that bacterial metabolites, like peptidoglycan, may play a role in promoting cardiac inflammation and ultimately leading to heart failure in mice following colitis. Methods: We induced colitis in C57 black mice with DSS (dextran sodium sulfate) treatment. Further, we collected fecal sample for 16s rRNA analysis to evaluate microbial flora. Additionally, heart and intestine tissues were collected to determine inflammatory genes expression (qPCR), immune cell infiltration (FACS), and intestinal permeability (WB and immunohistochemistry). Blood was collected to quantify the circulating peptidoglycan (PGN) level. The effect of PGN on NFkB inflammatory pathways was evaluated in macrophages. Results: Echo-data showed cardiac dysfunction in DSS mice. Masson’s trichrome staining showed deteriorated intestinal villi, while western blot data indicated decreased Occludin and increased PV-1 level, suggesting a leaky gut in DSS treated mice. Comparative analysis of 16s rRNA sequencing data showed significantly altered Firmicutes/Bacteroidetes ratio in DSS treated mice as compared to control mice. Moreover, plasma PGN level was significantly increased 3 weeks following DSS treatment. Real-time gene expression data of pro-inflammatory cytokines (TNFα) and fibrosis associated genes (col1α1, fibronectin) were increased in DSS mice heart. Increased CD68 expression in the immunostaining data of DSS mice heart suggests the recruitment of inflammatory macrophages. At the molecular level, CDK9 expression was significantly increased in macrophage following PGN treatment, which ultimately leads to an increase in NFkB signaling. Finally, CDK9 inhibition attenuated PGN-induced NFkB signaling. Conclusion: Together, our study suggests that colitis-induced gut leakage leads to CDK9-mediated NFκB upregulation, contributing to cardiac inflammation. Ongoing research will shed light on the underlying molecular mechanisms of cardiovascular dysfunction.

A Comparative Study of Microbial Communities, Biogenic Amines, and Volatile Profiles in the Brewing Process of Rice Wines with Hongqu and Xiaoqu as Fermentation Starters.

Rice wine is primarily crafted from grains through saccharification and liquification with the help of Qu. Qu plays an important role in the formation of the flavor quality of rice wine. Hongqu and Xiaoqu represent two prevalent varieties of Qu that are typically utilized in the brewing process of rice wine and play a crucial role in its production. In this study, GC, GC-MS, HPLC, and metagenomic sequencing techniques were used to contrast the microbial flora, biogenic amines, and aroma characteristics developed during the fermentation of rice wines, with Hongqu and Xiaoqu being used as initiating agents for the brewing process. The results show that the content of higher alcohols (including n-propanol, isobutanol, 3-methyl-1-butanol, and phenethyl alcohol) in rice wine brewed with Xiaoqu (XQW) was significantly higher than that in rice wine brewed with Hongqu (HQW). Contrarily, the concentration of biogenic amines in HQW surpassed that of XQW by a notable margin, but tyramine was significantly enriched in XQW and not detected in HQW. In addition, a multivariate statistical analysis revealed distinct disparities in the constitution of volatile components between HQW and XQW. Hexanoic acid, ethyl acetate, isoamyl acetate, ethyl caproate, ethyl decanoate, 2-methoxy-4-vinylphenol, etc., were identified as the characteristic aroma-active compounds in HQW and XQW. A microbiome analysis based on metagenomic sequencing showed that HQW and XQW had different dominant microorganisms in the brewing process. Burkholderia, Klebsiella, Leuconostoc, Monascus, and Aspergillus were identified as the primary microbial genera in the HQW fermentation period, while Pediococcus, Enterobacter, Rhizopus, Ascoidea, and Wickerhamomyces were the main microbial genera in the XQW brewing process. A bioinformatics analysis revealed that the concentrations of microbial genes involved in biogenic amines and esters biosynthesis were significantly higher in HQW than those in XQW, while the content of genes relevant to glycolysis, higher alcohol biosynthesis, and fatty acid metabolism was significantly higher in XQW than in HQW, which are the possible reasons for the difference in flavor quality between the two kinds of rice wine from the perspective of microbial functional genes.

Modulation of Microbiota and its Impact on Depression

Gut microbial flora is the largest micro-ecosystem in the human body, it is symbiotically associated with the host; and maintains normal physiological processes in a dynamic equilibrium state. A plethora of evidence supports that gut microbial flora influences the neurotransmitters of the central nervous system. This gut flora influences cognitive function, anxiety, depression; and mood disorders as they are capable of synthesizing neurotransmitters in the nervous system. Therefore intake of probiotics influences gut microbiome; and depression. The versatility and number of gut microbial flora varies individually, so the content of common gut microbes may affect the neurotransmitters, manipulating the gut microbiota with probiotics offers a novel approach to treat brain disorders such as depression via GUT-BRAIN AXIS. The present review outlines the aspect of such alterations and how modulation of gut microbiota influences depression.

Efficacy and Safety of Preoperative Prophylaxis in Cataract Surgery with Combined Topical Antiseptics: A Microbiological Study.

To evaluate the antimicrobial efficacy of an ophthalmic formulation containing hexamidine diisethionate (HD) 0.05%, polyhexamethylene biguanide (PHMB) 0.0001%, and edetate disodium (EDTA) 0.01% (Keratosept®, Bruschettini, Genova, Italy) on the microbial flora of a healthy ocular surface. Patients were enrolled consecutively. Each patient applied two drops of Keratosept® in the eye scheduled for cataract surgery (study eye) three times daily in the 2 days prior to surgery and one time in the morning of surgery. The contralateral eyes were considered as control (control eye). Bilateral conjunctival swabs were collected before the first administration (T0) and the morning of surgery (T1). The swabs were processed within 3 h from sampling for the automated detection of the presence of replicating microorganisms (colony-forming units, CFU/mL) and the provision of real-time growth curves. Conjunctival swabs of 32 patients (n = 128) were examined. Six patients were excluded from the efficacy analysis because of microbial load < 50 CFU/mL at T0 in the study eye. No difference between study and control eyes was observed at T0 (p = 0.40). Compared with T0, 20 (76.9%) study eyes and 10 (38.5%) control eyes showed a ≥ 1 log reduction of the microbial load at T1, with a significant difference between groups (p = 0.005). Keratosept® showed good tolerability, and no adverse events or eye discomfort were recorded. This study showed that the low-dose combination of antiseptic agents in the Keratosept® ophthalmic solution effectively reduces the bacterial load of healthy flora on the ocular surface.

Impact of Fixed Partial Dentures on Oral Microbial Flora and Gingival Health: An In Vitro Assessment of Salivary Microbial Profiles.

Introduction The oral cavity hosts diverse microorganisms affected by factors like pH, temperature, and oxygen levels, influencing disease potential. Dentists manage oral diseases and improve aesthetics using durable restorations. Understanding periodontal response to crowns and fixed partial dentures (FPDs) is essential for effective treatment. This study aims to assess the impact of FPDs on periodontal health by comparing microbial colonies in individuals with and without FPDs. The hypothesis is that there will be no difference in microbial flora and gingival health between the two groups. Materials and methods This in vitro study utilized 40 salivary samples divided into two groups: 20 from patients with FPDs/crowns (Group 1) and 20 from patients without (Group 2). Unstimulated saliva was collected, diluted, and cultured on nutrient agar and Sabouraud Dextrose Agar to quantify anaerobic bacteria and Candida colonies. Colony counts were scored from 0 to IV based on colony-forming unit (CFU), and microscopic examination identified the types of microbes present. Data were analyzed using an unpaired t-test with IBM SPSS Statistics for Windows, Version 26 (Released 2019; IBM Corp., Armonk, New York, United States), with significance set at p < 0.05. Results The independent t-test analysis showed significantly higher mean CFUs of anaerobic microbes in Group 1 (experimental) compared to Group 2 (control) (p = 0.000). However, mean CFUs of Candida did not significantly differ between groups (p = 0.194). Microscopic examination identified Enterococcus faecalis, Pseudomonas aeruginosa, Candida albicans, Staphylococcus aureus,and Streptococcus mutans in the experimental group, whereas the control group contained only Staphylococcus aureus and Streptococcus mutans. Conclusion Individuals with FPDs exhibit higher concentrations of anaerobic microbes and specific bacteria, suggesting an increased risk of gingival inflammation and emphasizing the importance of maintaining good oral hygiene.

A novel konjac rice formula with glucomannan and tapioca starch improve postprandial glycemic response – a randomized single-blind clinical trial

PurposeDue to white rice’s association with diabetes and other chronic diseases in many Asian countries, many industries are working to develop high-fiber rice substitutes with similar organoleptic characteristics. Konjac rice (KR) is a promising option, but maintaining its optimal fiber content for health benefits while preserving its ideal sensory profile remains a challenge. This study aims to investigate whether a KR formula, combining tapioca flour and glucomannan gel, possesses similar organoleptic attributes to white rice while preventing glycemic response elevation.Design/methodology/approachIn a six-week randomized single-blind clinical trial, 13 normoweight nondiabetic subjects received varying konjac-based rice and white rice ratios. Blood glucose levels were measured at intervals, and glycemic response was assessed using incremental area under the curve (iAUC). Visual analog scale gauged satiety, and organoleptic properties were evaluated.FindingsSubstituting white rice with pure and partial konjac-based rice significantly lowered postprandial blood sugar levels and glycemic response (p = 0.002). iAUC for pure KR and KR 1:1 was notably lower than white rice (p = 0.002). Subjects reported a sense of fullness comparable to white rice, with no significant organoleptic score differences (p = 0.260).Research limitations/implicationsThe study’s generalizability is compromised due to the limited number of participants, impacting external validity. The examined parameters offer a rough understanding of konjac grain’s impact on postprandial glycemic responses but do not elucidate underlying mechanisms or the duration of its inhibitory effect on glucose absorption. Long-term effects on metabolic, hormonal parameters and the colon’s microbial flora composition and function remain unexplored, constraining comprehensive insights into konjac grain’s extended implications.Practical implicationsThis study introduces a novel KR formula to address the escalating diabetes risks associated with white rice consumption. Substituting white rice with KR significantly reduces postprandial blood sugar levels, highlighting its potential in preventing type 2 diabetes (T2D). Tapioca flour enhances palatability, making KR a viable option. While promising, long-term effectiveness and safety require further research, emphasizing comprehensive lifestyle interventions. The study contributes valuable insights to innovative dietary strategies for prevalent health conditions, emphasizing the need for ongoing efforts in public health.Social implicationsWhite rice, a staple in Asian societies, is linked to a heightened risk of T2D due to increased production and inadequate dietary fiber. This connection contributes to the economic burden on governments through health insurance and lost productivity. Encouraging alternatives rich in fiber can mitigate this burden, offering a socioeconomically beneficial solution to preventable chronic diseases.Originality/valueThis trial demonstrates konjac-based rice’s potential in curbing glycemic responses, hinting at its role in preventing T2D. Glucomannan’s viscosity, satiety induction and potential gut health impact are highlighted. Further research is warranted for long-term effectiveness and safety. These findings contribute to the growing evidence supporting glucomannan as a valuable tool in addressing prevalent health conditions.

Supplementation of coated sodium butyrate relieved weaning stress and reshaped microbial flora in weaned lambs.

Weaning is an important period in the growth and development of lambs. Thus, effectively reducing the occurrence of weaning stress is critical for maintaining lamb production. Coated sodium butyrate has been shown to reduce inflammation, promote intestinal health, and maintain homeostasis. However, the application and potential mechanism of coated sodium butyrate in alleviating weaning stress in lambs are still unclear. To evaluate the effects of coated sodium butyrate on the growth performance, antioxidant capacity, and gut microbiota of weaned lambs, 10 weaned lambs of 21-day-old were randomly divided into two groups: the CON group (basal diet) and the NaB group (basal diet +3 g/kg of coated sodium butyrate). The trial lasted 21 days. The experimental results showed that compared to the CON group, coated sodium butyrate supplementation in the diet significantly increased the average daily weight gain and daily feed intake of lambs (p < 0.05). In addition, compared to the CON group, the addition of coated sodium butyrate also significantly decreased the serum MDA level of lambs (p < 0.05). Notably, the addition of coated sodium butyrate did not have a significant effect on the cecal microbiota, while increasing the diversity of colonic microbiota and promoting the abundance of Lachnospiraceae, Verrucomicrobiota, Akkermansia, Roseburia, and Sinobacteraceae, which are associated with the nutrient absorption of lambs (p < 0.05). These results indicate that dietary supplementation with coated sodium butyrate could promote the growth and antioxidant capacity of weaned lambs and alleviate weaning stress.

Colonization of construction materials by microbial flora of the oral cavity and its significance for prosthetics

Today there is no unified personalized approach to the selection of construction materials in orthopedic dentistry, hence it is necessary to introduce and further study the effect of construction materials on the microbiota of the oral cavity. In particular, principles of microbial adhesion to various construction materials, development of biofilm and bacterial colonization of concrete construction materials need to be analysed. The article provides a review of literature devoted to the description of the adhesive properties of oral microbiota to orthopedic materials from the groups of polymers, metals and ceramic constructions as well as the development of the negative impact of the construction material on the microbiota of a particular group of teeth and the general condition of the microflora of the oral cavity, increase of the risks of dental plaque formation, biofilms formation and a shift of microflora towards the predominance of cariogenic one.

From Gut Microbiomes to Infectious Pathogens: Neurological Disease Game Changers.

Gut microbiota and infectious diseases affect neurological disorders, brain development, and function. Compounds generated in the gastrointestinal system by gut microbiota and infectious pathogens may mediate gut-brain interactions, which may circulate throughout the body and spread to numerous organs, including the brain. Studies shown that gut bacteria and disease-causing organisms may pass molecular signals to the brain, affecting neurological function, neurodevelopment, and neurodegenerative diseases. This article discusses microorganism-producing metabolites with neuromodulator activity, signaling routes from microbial flora to the brain, and the potential direct effects of gut bacteria and infectious pathogens on brain cells. The review also considered the neurological aspects of infectious diseases. The infectious diseases affecting neurological functions and the disease modifications have been discussed thoroughly. Recent discoveries and unique insights in this perspective need further validation. Research on the complex molecular interactions between gut bacteria, infectious pathogens, and the CNS provides valuable insights into the pathogenesis of neurodegenerative, behavioral, and psychiatric illnesses. This study may provide insights into advanced drug discovery processes for neurological disorders by considering the influence of microbial communities inside the human body.

Chemical profile by GC-MS and protective effect of Algerian cloves (Syzygium aromaticum) against Lactobacillus spp. and Streptococcus spp. isolated from dental caries

The oral cavity is home to a large and diversified microbial flora, which plays an important role in the genesis of multiple diseases, including tooth decay. Indeed, tooth decay is the most common ailment in the world, with almost everyone having experienced it at least once in their lifetime. Examining plants used in traditional medicine is one of the research approaches used to discover novel, potent antibacterial chemicals with a broad spectrum of action, as present antibacterials have significant drawbacks. This study aims to examine the chemical composition of Algerian clove Syzygium aromaticum using GC-MS and to evaluate the antibacterial activity of the methanol extract against bacteria isolated from dental caries caused by Streptococcus spp. and Lactobacillus spp. The results show a strong extraction yield of 29.7%, with high amounts of polyphenols and flavonoids calculated at 178.82 mg GAEQ/g and 24.13 mg QEQ/g. The principal chemical elements of S. aromaticum peel methanol extract were identified as eugenol (61.23%) and eugenol acetate (26.45%) based on mass spectrum data and retention times. The methanol extract has a significant antibacterial effect against tested strains, with MICs ranging from 111.37 to 445.5 mg/mL. Higher concentrations of polyphenols resulted in a significant increase in inhibition zone diameter against S1 (r2 = 0.94, p &amp;lt; 0.001), L3 (r2 = 0.94, p &amp;lt; 0.001), L5 (r2 = 0.93, p &amp;lt; 0.001), and L9 (r2 = 0.96, p &amp;lt; 0.001).

A comparative analysis of the antimicrobial efficacy of different concentrations of silver nanoparticles and chlorhexidine against Enterococcus faecalis - In vitro study

Introduction: Root canal irrigants combat endodontic microbial flora, effectively targeting resilient microbes like Enterococcus faecalis, which persist despite treatment. Chlorhexidine (CHX), as an irrigant, boasts broad-spectrum antibacterial activity, notable substantivity in dentine, and favorable biocompatibility. In the realm of nanotechnology, the use of silver nanoparticle (AgNP) solutions appears promising, given their strong antibacterial and antimicrobial properties. Objective: The objective of the study was to compare the antimicrobial effectiveness of varying concentrations (1%, 0.75%, and 0.5%) of AgNPs and 2% CHX against E. faecalis. Materials and Methods: Four irrigant solutions, including 1%, 0.75%, and 0.5% AgNPs and 2% CHX with gentamicin as the control, will be assessed. The ATCC 29212 strain of E. faecalis will be cultivated at 37°C on brain–heart infusion Broth plates. After 48 h, the bacterial population will be quantified by counting colony-forming units on agar plates. Each irrigant will be applied to the plates, and the resulting bacterial inhibition zone will be measured. Results: Our study demonstrates the antimicrobial effects of 1%, 0.75%, and 0.5% AgNPs and 2% CHX against E. faecalis. While 2% CHX exhibited superior activity, AgNPs at various concentrations also displayed inhibitory effects against E. faecalis. Conclusion: In conclusion, this study highlights the antimicrobial properties of 1%, 0.75%, and 0.5% AgNPs and 2% CHX against E. faecalis. The results indicate that 2% CHX exhibits superior activity, while AgNPs at various concentrations also demonstrate inhibitory zones against E. faecalis.

Comparative Evaluation of Disinfection Protocols for Dental Impressions in Prosthodontics.

In prosthodontics, dental impressions are essential for creating precise dental restorations. However, these impressions are susceptible to microbial contamination, which can pose a risk of infection to patients. Consequently, effective disinfection methods are crucial to prevent postoperative infections. This study aims to evaluate the effectiveness of various disinfection techniques for dental impressions used in prosthodontics. A total of 148 poured dental impressions were randomized into three disinfection groups: immersed in 0.5% sodium hypochlorite, 2% glutaraldehyde, or 0.2% chlorhexidine solution. The bacterial contamination was evaluated by direct colony-forming unit (CFU) counting, while the dimensional accuracy and surface detail duplication of each resin sample were determined as physical properties. Data were analyzed using SPSSversion 23.0 (IBM Corp., Armonk, NY). Either analysis of variance (ANOVA) with the option for post-hoc or non-parametric tests was used to investigate and compare the efficacy of the better disinfection protocols where the p-value was considered significant if less than 0.05. Glutaraldehyde showed the lowest mean CFU count (2.5 log10 CFUs), followed by sodium hypochlorite (3.2 log10 CFUs) and chlorhexidine (3.5 log10 CFUs). All disinfection protocols were able to significantly reduce microbial contamination when compared with the control group (p < 0.05). The results of the physical property assessment demonstrated acceptable dimensional accuracy in all tested protocols, with slight differences recorded between them regarding the reproduction of surface detail. More specifically, the mean dimensional deviation was in the range between 0.02 and 0.04 mm, while scores for surface detail reproduction ranged from 2 to 4. The ANOVA results revealed significant differences in microbial contamination levels (F(2, 145) = 5.72, p = 0.007) and dimensional accuracy (F(2, 145) = 3.45, p = 0.032) between the various disinfection protocols. This study enlightens the effective sterilization protocol to be adopted in prosthodontics for dental impressions. Glutaraldehyde was most effective in microbial reduction, while sodium hypochlorite and chlorhexidine were equally effective. Therefore, clinicians must be vigilant in assessing the type of microbial flora that can be encountered during prosthodontic procedures while choosing disinfection protocols for patient safety and quality of impressions.

Peculiarities of the examination of citrus fruits (mandarins) during the forensic examination

In today’s environment, the issue of ensuring the identification and safety of imported products is of particular relevance, which is aimed at the successful functioning of the state in the global economy and the reduction of technical barriers to trade. The article discusses the peculiarities of conducting an examination of citrus fruits, in particular, tangerines, with a view to their further classification in the Ukrainian Classifier of Goods for Foreign Economic Activity. Approaches to the classification of citrus fruits in the process of commodity examination are summarized. The diagnostic tasks to be solved by the forensic expert during the study of citrus fruits are defined, which will allow to have more analytical information from this category of goods. The main identification tasks of the commodity examination of citrus fruits, in particular mandarins, are: determination of the variety or species of mandarins, detection of certain quality markers, determination of origin, assessment of the degree of freshness and maturity, detection of deviations from standards. Modern methods of analysis are indicated, which allow to carry out research on tangerines at the molecular, chemical and morphological levels in order to obtain objective information about their quality, safety and other characteristics that may be important in court proceedings. The main aspects of microbiological methods during the examination include: detection of pathogenic microorganisms (analysis for the presence of pathogenic bacteria such as Salmonella, E. coli and Listeria monocytogenes), determination of the level of the total microbial flora, assessment of the degree of rot and mold, determination of the effectiveness of preservatives and other preservation methods, assessment of compliance with food safety standards. Systematization of information data on characteristic defects of citrus fruits (tangerines), namely: rot, mold, mechanical damage, weak skin color, increased acidity, defects in shape or size (identified defects are of natural origin) is presented. Defects that are characteristic of low-quality products with signs of deterioration: rot, mold, mechanical damage, signs of drying or desiccation, heterogeneity in color and texture. The mechanism of forensic commodity examination of citrus fruits (tangerines) is described.