The recent emergence of many new instruments andmethods for optical noninvasive diagnostics is accompanied, with rare exceptions, by the fact that their adoptionand use in practice are implemented without full metrological assurance [13]. Among them is the method oflaser Doppler flowmetry (LDF) allowing estimation ofblood flow intensity in the microcirculation link of thebloodstream as well as detection and study of collectiverhythmic processes of blood microcirculation. One way toincrease the level of metrological assurance is to developoptical phantoms (test objects) reproducing the measuredquantities and/or registered biomedical parameters [4, 5].According to its physical meaning, the measurementresults in LDF –microcirculation index (MI) – is a valuemeasured in relative (perfusion) units, which is proportional to the average concentration of red blood cellensemble and their average velocity [6]. In LDF devices,this quantity is determined by probing biological tissue bylaser radiation in the wavelength range of 6301100 nmand measuring the Doppler shift frequency in the range of2024,000 Hz arising after the reflection of radiation froman ensemble of red blood cells moving at different speedsin the range 0.110 mm/s in small vessels – arterioles,capillaries, and venules [7]. The current method of LDFis in fact the only method that allows local study of capillary tissue perfusion intensity quickly and noninvasively.One of the main diagnostic applications of LDF is analysis of capillary microcirculation rhythms in the range of0.011.8 Hz [8].The main constraint for development of the LDFmethod is the unsatisfactory reproduction of the size ofthe signal recorded by LDF, which is used for the purposeof setup and calibration at the production stage, as well asto check the current metrological state during operation[9]. The most widespread method of LDF signal reproduction using a stabilized suspension of lightscatteringparticles undergoing Brownian motion [10] has a numberof disadvantages that make it of little use for practicalapplications: low stability, short shelf life, extreme sensitivity to external influencing factors – temperature andvibration especially, and the sample is able to reproduceonly one level of signal. The combination of these factorsleads to the fact that in the Russian Federation LDFdevices are not subject to state metrological control during their operation (procedures carried out to identify thelimit state or latent failure), which often leads todecreased confidence of medical doctors in the method.Thus, of critical importance are theoretical foundations of LDF signal reproduction, as well as its practicalimplementation in the form of a device (test object) suitable for metrological state control (MSC) not only at theproduction stage (adjustment, final checking, testing ofaccuracy how the specified static and dynamic characteristics of the instrument match its individual characteristics), but also during the operational phase in the health