Applicable Method Research Articles

Effect of a brush tip on in-office bleaching gels in an attachable syringe: Hydrogen peroxide penetration, bleaching efficacy and amount of gel expended

ObjectiveEvaluate the penetration of hydrogen peroxide (HP) into the pulp chamber, bleaching efficacy (BE) and amount of gel expended during in-office bleaching using an applicator brush tip and conventional tip from different commercial brands. Materials and Methods104 human premolars were randomly distributed into thirteen groups (n = 8) according to the commercial brand: DSP White Clinic 35 % Calcium (DW), Nano White 35 % (NW), Total Blanc One-Step 35 % (TS), Whiteness HP Blue 35 % (WB), Potenza Bianco Pro SS 38 % (PB), Opalescence XTra Boost 40 % (OB), no bleaching (negative control), and application method: applicator brush tip and conventional tip for all groups. Initial HP concentration (%) was determined via titration and pH was measured with digital pH meter. Concentration (µg/mL) of HP into the pulp chamber was measured using UV–Vis spectrophotometry, the BE (ΔE*ab, ΔE00 and ΔWID) was evaluated with a digital spectrophotometer, and the amount of gel expended was evaluated using a precision analytical digital balance. Statistical analysis included two-way ANOVA, Tukey's, and Dunnett's test. Comparison between HP into the pulp chamber vs BE was performed with Person's correlation (α = 0.05). ResultsBrush tip demonstrated a low amount of HP in the pulp chamber compared to the conventional method for all bleaching gels (p < 0.0003), as well as lower amount of gel expended (p < 0.002). The brush tip did not result in a significant difference in BE compared to the conventional (p > 0.05). No correlations were found between both factors (p > 0.05). ConclusionBrush tip showed lower penetration of HP in the pulp chamber and a reduced volume of spent gel when compared to the conventional tip, for all commercial brands. Clinical relevanceBrush tip is recommended for bleaching gels in an attachable syringe due to its ability to reduce the penetration of HP into the pulp chamber and minimize the amount of bleaching gel used.

Spectator Exciton Effects in Nanocrystals III: Unveiling the Stimulated Emission Cross Section in Quantum Confined CsPbBr3 Nanocrystals.

Quantifying stimulated emission in semiconductor nanocrystals (NCs) remains challenging due to masking of its effects on pump-probe spectra by excited state absorption and ground state bleaching signals. The absence of this defining photophysical parameter in turn impedes assignment of band edge electronic structure in many of these important fluorophores. Here we employ a generally applicable 3-pulse ultrafast spectroscopic method coined the "Spectator Exciton" (SX) approach to measure stimulated-emission efficiency in quantum confined inorganic perovskite CsPbBr3 NCs, the band edge electronic structure of which is the subject of lively ongoing debate. Our results show that in 5-6 nm CsPbBr3 NCs, a single exciton bleaches more than half of the intense band edge absorption band, while the cross section for stimulated emission from the same state is nearly 6 times weaker. Discussion of these findings in light of several recent electronic structure models for this material proves them unable to simultaneously explain both measures, proving the importance of this new input to resolving this debate. Along with femtosecond time-resolved photoluminescence measurements on the same sample, SX results also verify that biexciton interaction energy is intensely attractive with a magnitude of ∼80 meV. In light of this observation, our previous suggestion that biexciton interaction is repulsive is reassigned to hot phonon induced slowdown of carrier relaxation leading to direct Auger recombination from an excited state. The mechanism behind the extreme slowing of carrier cooling after several stages of exciton recombination remains to be determined.

Cascading Effects of Boron Application Methods on Nutrient Distribution and Metabolite Profiles in Pear Trees

Cascading Effects of Boron Application Methods on Nutrient Distribution and Metabolite Profiles in Pear Trees

An Overview of Advanced Antimicrobial Food Packaging: Emphasizing Antimicrobial Agents and Polymer-Based Films.

The food industry is increasingly focused on maintaining the quality and safety of food products as consumers are becoming more health conscious and seeking fresh, minimally processed foods. However, deterioration and spoilage caused by foodborne pathogens continue to pose significant challenges, leading to decreased shelf life and quality. To overcome this issue, the food industry and researchers are exploring new approaches to prevent microbial growth in food, while preserving its nutritional value and safety. Active packaging, including antimicrobial packaging, has gained considerable attention among current food packaging methods owing to the wide range of materials used, application methods, and their ability to protect various food products. Both direct and indirect methods can be used to improve food safety and quality by incorporating antimicrobial compounds into the food packaging materials. This comprehensive review focuses on natural and synthetic antimicrobial substances and polymer-based films, and their mechanisms and applications in packaging systems. The properties of these materials are compared, and the persistent challenges in the field of active packaging are emphasized. Specifically, there is a need to achieve the controlled release of antimicrobial agents and develop active packaging materials that possess the necessary mechanical and barrier properties, as well as other characteristics essential for ensuring food protection and safety, particularly bio-based packaging materials.

DEM super-resolution guided by shaded relief using attention-based fusion

Deep-learning based approaches have been proven effective for Digital Elevation Model (DEM) super-resolution (SR) tasks. Previous networks typically treat DEM elevation values as single-channel image for input. However, DEM images alone cannot fully capture spatial and terrain features. Shaded relief images (SRIs), derived from DEMs, serve as crucial visual cues that intuitively convey terrain characteristics, addressing the limitations of DEM images and providing synergistic benefits for training DL models. The primary challenge in utilizing SRIs for guiding DEM SR lies in accurately selecting a consistent structure to extract and effectively integrate features from SRIs and DEMs. In this study, we propose an Attention-based Hierarchical Terrain Fusion (AHTF) framework for guided DEM SR. Specifically, an Attention-based Feature Fusion Module (AFFM) is designed to efficiently fuse relevant information from LR DEM and SRI, which includes a feature enhancement block to select valuable features and a feature recalibration block to fuse diverse terrain features. Additionally, we optimize the loss function from the perspectives of terrain analysis and visual effects. We validate AHTF on our newly constructed real-world Shade-DEM SR dataset and two open-source DEM SR datasets. Compared to the current state-of-the-art methods, our AHTF achieves the best results in terms of root mean square error (RMSE) for elevation, slope, and aspect. Furthermore, the extracted stream networks are closer to real-world conditions. This study offers new insights and methods for further research and application in the field of DEM super-resolution. Our dataset can be obtained at https://doi.org/10.6084/m9.figshare.25590945.

Метод поєднання ливарного та термічного процесів виготовлення виливків із залізовуглецевих сплавів

With the help of innovations at an industrial enterprise, it is possible to increase labor efficiency and reduce the resource intensity of production. The relevance of the development of the technology of metal casting in sand molds, as the most common casting process, is justified by the fact that up to 80 % of the tonnage of castings is produced in such molds, including special types of casting in molds from sand materials. 60-70 % of the equipment capacities, areas, and personnel of foundry shops are attributed to molding processes, including mixing and rod production. Pollution "due to the mold" can reach up to 80%. A significant potential for the development of foundry mold technology is the improvement of the processes of casting metal into molds made of loose sand without a binding component, in particular, according to the Lost Foam Casting Process (LFC). The authors present the concept of improving the processes of casting metal into molds made of loose sand by the method of dual application of molding materials and give examples of such application. The use of loose molding sand for rapid cooling of the casting, including heat treatment, is able to strengthen its metal and reduce the duration of cooling of the casting in the mold, which leads to an increase in the productivity of foundries without increasing the number of furnace equipment and loose mold filler. The applied method of isothermal hardening (austempering) from the hot state of castings from iron-carbon alloys excludes heating with exposure for austenization of castings, which reduces the cost, time and energy consumption of obtaining products with a strengthened bainite structure, which, in fact, corresponds to the concept of realizing internal reserves as a foundry sand mold, and cast metal. Some foundry enterprises of the Vinnytsia region also use LFC or have concluded contracts, planning to reconstruct their shops for this ecological and economically beneficial foundry technology.

Molecular Connectomics Reveals a Glucagon-Like Peptide 1 Sensitive Neural Circuit for Satiety.

Liraglutide and other agonists of the glucagon-like peptide 1 receptor (GLP-1RAs) are effective weight loss drugs, but how they suppress appetite remains unclear. One potential mechanism is by activating neurons which inhibit hunger-promoting Agouti-related peptide (AgRP) neurons of the arcuate hypothalamus (Arc). To identify these afferents, we developed a method combining rabies-based connectomics with single-nuclei transcriptomics. Applying this method to AgRP neurons predicted at least 21 afferent subtypes in the mouse mediobasal and paraventricular hypothalamus. Among these are Trh+ Arc neurons, inhibitory neurons which express the Glp1r gene and are activated by the GLP-1RA liraglutide. Activating Trh+ Arc neurons inhibits AgRP neurons and feeding in an AgRP neuron-dependent manner. Silencing Trh+ Arc neurons causes over-eating and weight gain and attenuates liraglutide's effect on body weight. Our results demonstrate a widely applicable method for molecular connectomics, comprehensively identify local inputs to AgRP neurons, and reveal a circuit through which GLP-1RAs suppress appetite.

A new computed tomography-based approach to quantify swallowing muscle volume by measuring tongue muscle area in a single slice.

Measuring the swallowing muscle mass with volume measurements is complex and time intensive; therefore, it is not used in clinical practice. However, it can be clinically relevant, for instance, in the case of sarcopenic dysphagia. The aim of the study was to develop a feasible and clinically applicable method to measure swallowing muscle mass. Data from 10 head and neck cancer patients were collected from the Oncological Life Study data-biobank of the University Medical Center Groningen. The pharyngeal constrictor, genioglossus, mylohyoid and geniohyoid complex muscles, as well as the tongue complex muscles, were delineated manually on routinely performed head and neck computed tomography scans. Axial and sagittal planes were used for volume and area measurements, respectively. Muscle density measurements were performed with and without Hounsfield unit thresholding. Correlations were assessed by Pearson correlation coefficients, and interobserver reliability was measured using intra-class correlation coefficients (ICCs). Significant differences were observed between sagittal area measurements with and without Hounsfield unit thresholds for pharyngeal constrictor, tongue complex and the sum of the swallowing muscles (t>6; P-value<0.001). Stronger correlations emerged without Hounsfield unit thresholding. Strong positive and significant correlations were found between the total swallowing muscle mass volume and the sagittal area of the tongue complex muscles (r=0.87, P-value<0.05) and the sum of the sagittal areas of the pharyngeal constrictor and tongue complex muscles (r=0.85, P-value<0.05). The use of the Hounsfield unit threshold weakened correlations. Interobserver reliability was assessed and found to be fair to good for the pharyngeal constrictor muscle (ICC=0.68, P-value<0.05), excellent for the tongue complex muscles (ICC=0.98, P-value<0.05) and excellent for the total swallowing muscle area (ICC=0.96, P-value<0.05). Single-slice delineation of the sagittal area of tongue complex muscle and pharyngeal constrictor muscle is a promising, fast, simple and clinically applicable method for measuring the total volume of the swallowing muscle mass in head and neck cancer patients without Hounsfield unit thresholding. These advancements and findings would help in the early and accurate diagnosis of definitive sarcopenic dysphagia.

Strain-Release-Driven Modular Synthesis of Oxetane-Based Amide Bioisosteres: Concise, Robust and Scalable Approach.

Amide bioisoterism is a widely used strategy in drug development to fine-tune physicochemical, pharmacokinetic, and metabolic properties, eliminate toxicity and gain intellectual property rights in uncharted chemical space. Of these, oxetane-amines offer particularly exciting possibilities as bioisosteres, although they are less frequently investigated than warranted due to the lack of simple and widely applicable synthetic methods. Herein, we report a two-step, practical, modular, robust, and scalable method for the construction of oxetane-containing amide bioisosteres that relies on the readily available oxetan-3-one. This operationally simple procedure exploits the enhanced reactivity of the keto group of the commercially available oxetan-3-one to form amine-benzotriazole intermediates, which springloaded adducts are then reacted with various aliphatic and aromatic organometallic reagents under mild conditions to afford various amino-oxetanes in good to high yields. The simplicity and broad applicability of the method greatly facilitates the synthesis of derivatives that were previously difficult or impossible to produce. The usefulness of this method in the field medicinal chemistry was also demonstrated by eliminating the well-known metabolic problem of ketoconazole.

Endophytic bacterium Sphingomonas panaciterrae NB5 influences soil properties and improves growth, nutrient contents, and yield of red amaranth (Amaranthus tricolor L.)

Plant growth promoting rhizobacteria (PGPR) are crucial for enhancing plant growth and restoring soil health. Despite the excellent plant growth promoting traits, information is limited on the efficacy of Sphingomonas as a PGPR, especially in vegetable crops. In this study, we used Sphingomonas panaciterrae NB5 as a biofertilizer in leafy vegetable red amaranth in three methods: seed priming (SP), root drenching + foliar (RD + FA), and bacterial culture filtrate (BCF) foliar application. Bio-inoculation of NB5 significantly increased the plant height, number of leaves, leaf area, stem girth, total chlorophyll, vitamin C, and antioxidant contents of red amaranth in all methods of application. Bacterial treatment resulted in notable alterations to the root structure, consisting of the formation of secondary, tertiary, and fibrous roots, particularly in the BCF foliar application and RD + FA treatment.The fresh and dry biomass significantly increased both in root and shoot, resulting in improved yield. The nutritional profile revealed that bacterial application significantly increased the nitrogen, potassium, magnesium, iron, and zinc content, with a slight increase in phosphorus content, in shoots and roots in all the methods of bacterial application compared to control. In post-harvest soil, NB5 boosted total nitrogen, available phosphorus, calcium, and sulfur, as well as soil organic carbon (SOC) and total bacterial populations, regardless of the application methods. The RD+FA treatment outperformed the other methods of application in most of the plant and soil parameters, and the next was the BCF foliar application. Multivariate analysis also confirmed the better performance of RD+ FA and BCF foliar applications. Therefore, simultaneous application of NB5 through root drenching and foliar application could be recommended to the farmers for increasing the yield of red amaranth with improved nutrients and restoring soil health and productivity.

Experimental study on manufacturing of vacuum-brazed grinding tools with diamond grains

AbstractThis work provides an experimental study on manufacturing methods for vacuum-brazed diamond grinding for edge machining of carbon fiber-reinforced polymers (CFRP). Therefore, various methods for applying active brazing alloys and diamond grains were investigated. The application methods were evaluated regarding practicability and the possibility of automation. Additionally, the samples were examined after vacuum brazing and brazing results were compared concerning method, amount and type of brazing alloy, diamond grains and binding agent. It was found that the best results could be achieved by using fluidized bed coating for diamond application and a brazing alloy containing a 15% binding agent. The method and brazing alloy were then used to produce cylindrical mounted points for grinding tests. It was possible to show that vacuum-brazed tools performed as good as commercially available electroplated tools.

Development of a New Method for Evaluating Heat and Moisture Exchanger Performance.

A model system described in International Organization for Standardization 9360 is the standard method for estimating the humidifying performance of heat and moisture exchangers (HMEs). However, there are no reliable bedside methods for evaluating the ongoing humidification performance of HMEs. Therefore, this study aimed to develop 2 clinically applicable methods for estimating the ongoing humidifying performance of HMEs and to evaluate their reliability in a model system. Physiologically expired gas was simulated using a heated humidifier, and ventilation was delivered using a ventilator with constant flow through 3 different types of HMEs. Relative humidity (RH) was measured using a capacitive-type moisture sensor. Water content lost during expiration was calculated by integrating absolute humidity (AH), instantaneous gas flow measured at the expiratory outlet of the ventilator, and time. We also calculated the water content released and captured by the HMEs during tidal ventilation by integrating the difference in AH across the HMEs, instantaneous gas flow, and time. We found that the RH, temperature, and AH were almost constant on the expiratory outlet of the ventilator but rapidly varied near the HMEs. The water content lost by the 3 HMEs was associated with the manufacturer-reported values and inversely correlated with the calculated values of the water content exchanged by the HMEs. The water content released and captured by HMEs was closely correlated with the difference in HME weight measured at the end of inspiration and expiration; however, the water content captured by HMEs seemed to be overestimated. Our results demonstrated that our system was able to detect the differences in the performance of 3 models of HMEs and suggest that our method for calculating water loss is reliable for estimating the water retention performance of HMEs during mechanical ventilation, even in the presence of a constant flow.

A Review of Research Progress in Microfluidic Bioseparation and Bioassay.

With the rapid development of biotechnology, the importance of microfluidic bioseparation and bioassay in biomedicine, clinical diagnosis, and other fields has become increasingly prominent. Microfluidic technology, with its significant advantages of high throughput, automated operation, and low sample consumption, has brought new breakthroughs in the field of biological separation and bioassay. In this paper, the latest research progress in microfluidic technology in the field of bioseparation and bioassay is reviewed. Then, we focus on the methods of bioseparation including active separation, passive separation, and hybrid separation. At the same time, the latest research results of our group in particle separation are introduced. Finally, some application examples or methods for bioassay after particle separation are listed, and the current challenges and future prospects of bioseparation and bioassay are discussed.

The Place and Approaches of Family Medicine Doctors in the Health Management of CBRN Events: The Case of Türkiye

In this study, it was aimed to determine the level of knowledge of Family Medicine residents about Chemical, Biological, Radiological and Nuclear (CBRN) events, their risk assessments and their thoughts on the roles that CBRN events can play in healthcare services. As a multi-center and cross-sectional analytical study, 444 family medicine residents at the national level were included in the study via an online survey application method. The data form included a total of 25 questions prepared to evaluate the sociodemographic information of the participants and their evaluation, awareness, knowledge level, approach to events and general opinions regarding CBRN event risks. 91.7% of physicians evaluated their knowledge level as "very little" or "little" and 95.7% evaluated their preparation level as "very little" or "little". 77.3% of physicians think that family physicians should have a role in CBRN incidents. For a possible CBRN event in the next 5 years, physicians predict biological events as the highest risk and nuclear events as the lowest risk. The rate of physicians who stated that they had previously received CBRN training was 17.8%, and the rate of those who participated in the CBRN drill was only 0.9%. While 89.9% of the participants wanted to receive training on health management in CBRN incidents; 80.2% want the training to be included in the specialty curriculum. While the participants' level of knowledge about the symptoms that may develop after a CBRN event is high, when asked about the awareness of CBRN risks around their institutions, 79.3% of physicians stated that they were not aware. It was determined that the level of knowledge, preparation, training, practice and awareness of family physicians regarding CBRN events was low. On the other hand, their willingness to receive training and their belief that they had a role in CBRN incidents were found to be high. According to the data of our study, we evaluate that there is a need for institutional structuring against CBRN risks, including primary health care services, and we suggest that CBRN training modules should be included in specialty curricula, starting from medical faculties.

Arsenic and Zinc Accumulation in Zinc-Biofortified Wheat under Arsenic-Contaminated Irrigation and Varied Zinc Application Methods

Arsenic and Zinc Accumulation in Zinc-Biofortified Wheat under Arsenic-Contaminated Irrigation and Varied Zinc Application Methods

Method for layout optimization of coastal cage aquaculture systems in Southeast Asia

This paper presents the development of a generally applicable method for the layout optimization of coastal cage aquaculture systems in Southeast Asia. The method combines data from the authors’ spatial-planning methodology with results of water quality modelling and an optimization technique based on genetic algorithms. This study successfully demonstrates the method's effectiveness for a target aquaculture site in northwest Bali, Indonesia. Optimum siting and sizes of fish farms to maximize fish production and promote fish growth while securing environmental sustainability are delivered for the site in Bali. The results of the application for several fish farming sizes show that by proper fish farming layout, environmentally sustainable fish production on the western channel of the site in Bali could rise tremendously with respect to the current operating conditions. Fish farm clusters with 256 cages are found to be best suited to the western channel. The method has broad applicability, can be upscaled to new sites, and can be used to assess, expand, and optimize currently operating sites.

A bacteria-based index of biotic integrity indicates aquatic ecosystem restoration

Intensive ecological interventions have been carried out in highly polluted shallow lakes to improve their environments and restore their ecosystems. However, certain treatments, such as dredging polluted sediment and stocking fish, can impact the aquatic communities, including benthos and fishes. These impacts can alter the composition and characteristics of aquatic communities, which makes community-based ecological assessments challenging. Here we develop a bacteria-based index of biotic integrity (IBI) that can clearly indicate the restoration of aquatic ecosystems with minimal artificial interventions. We applied this method to a restored shallow lake during 3-year intensive ecological interventions. The interventions reduced nutrients and heavy metals by 27.1% and 16.7% in the sediment, while the total organic carbon (TOC) increased by 8.0% due to the proliferation of macrophytes. Additionally, the abundance of sulfur-related metabolic pathways decreased by 10.5% as the responses to improved ecosystem. The score of bacteria-based IBI, which is calculated based on the diversity, composition, and function of benthic bacterial communities, increased from 0.62 in 2018 to 0.81 in 2021. Our study not only provides an applicable method for aquatic ecological assessment under intensive artificial interventions but also extends the application of IBI to complex application scenarios, such as ecosystems with significantly different aquatic communities and comparisons between different basins.

Application Methods of Qing Dynasty Cloud Shoulders in Modern Women's Fashion

Application Methods of Qing Dynasty Cloud Shoulders in Modern Women's Fashion

Development of bioinsecticide based on Streptomyces griseoflavus PAL114 for control of black bean aphids Aphis fabae

ABSTRACT This research highlights the efficiency of a bioinsecticide based on Streptomyces griseoflavus PAL114 in controlling black bean aphids and its effect on fava beans. Three actinobacterial strains were tested in vitro for their aphicidal activity. The PAL114 strain was then formulated in talc powder and tested again. Formulation processes were performed with four spore suspension densities (102, 104, 106 and 108 spore ml−1) at a rate of 25 ml per 100 g of talc powder. Furthermore, the in vivo effect of bioinsecticide on fava bean plants was studied in pot experiments using two application methods: spray and spray + soil amendment. The results showed that only the PAL114 strain had a highly significant effect on mortality (p = .001). Their high-density formulations at 106 and 108 spore ml−1 induced very highly significant activity (p < .001) for both, with no influence difference exceeding 90% after 58 h. In contrast, the other low-density formulations at 102 and 104 spore ml−1 had no significant effect (p = .322 and p = 1.000, respectively). There was no adverse effect of the bioinsecticide on fava bean; instead, there was an improvement in growth, especially when the spray application was combined with soil amendment. The present study opens up prospects for field studies on the biocontrol of this crop pest.

On the Practical Application of Contextual Teaching in Higher Vocational Preschool Education English Classrooms

Contextual teaching is a new instructional model emerging under the innovative backdrop of educational theories and methodologies in modern times. In the context of higher vocational colleges specializing in preschool English education, this approach is particularly conducive to creating a favorable teaching environment and atmosphere. It enables students to actively engage in the learning process, thereby enhancing their subsequent application of English knowledge. This article primarily analyzes the advantages of contextual teaching in the field of preschool English education at higher vocational colleges. Furthermore, it discusses practical application methods of contextual teaching in this specific educational context, aiming to provide insights for enhancing the learning capabilities of early childhood English educators.