The improvement of water quality has been the major focus of the research community due to the rapid contamination of water bodies by anthropogenic and other activities. Microalgae can acclimatize to these environments by using some specific mechanisms. In the present study, photosynthetic eukaryote H2 isolated from pond water collected from the Hoshiarpur district (Punjab) was acclimatized by exposing it to the highly polluted wastewater of Buddha Nala over 30 days. Thereafter,the biomass of the dominating acclimatized microalgae consortium was harvested i.e. A1, and elucidated for biochemical and proximate compositionfor its potential applications. The treatment efficacy of H2 and acclimatized microalgae consortium A1 was compared based onthe reduction of physico-chemical parameters of treated wastewater. The study revealed adapted microalgae consortium A1 with more potential which may be due to higher biomass production and lesser lag phase time in contaminated wastewater. Hence, the findings of the study underscore the astounding ability of the acclimatized microalgae consortium A1 to biotreat highly polluted Buddha Nala wastewater.