You have accessJournal of UrologyBenign Prostatic Hyperplasia: Medical & Hormonal Therapy1 Apr 2014PD23-08 COMBINATION OF TAMSULIN (0.4 MG) AND DUTASTERIDE (5 MG) FOR BENIGN PROSTATIC HYPERPLASIA. OBJECTIVE ANALYSIS OF STROMAL COMPONENTS OF THE TRANSITION ZONE Andre Cavalcanti, Felipe Restrepo, Waldemar Costa, Carla Gallo, Bruna Pereira, Nathalia Machado, and Francisco Sampaio Andre CavalcantiAndre Cavalcanti More articles by this author , Felipe RestrepoFelipe Restrepo More articles by this author , Waldemar CostaWaldemar Costa More articles by this author , Carla GalloCarla Gallo More articles by this author , Bruna PereiraBruna Pereira More articles by this author , Nathalia MachadoNathalia Machado More articles by this author , and Francisco SampaioFrancisco Sampaio More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1845AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We conducted an objective (stereological) histological analysis of the stromal components of the transition zone of benign prostatic hyperplasia (BPH) patients using or not using a combination of tamsulin (0.4-mg) and dutasteride (5-mg). METHODS BPH samples were analyzed in the transition zone of 5 patients submitted to open surgery without previous treatment (control group) and 5 patients with BPH, submitted to open surgery, which underwent treatment with tamsulosin + dutasteride for 3 months prior to surgery. The samples were removed after open surgery, and fixed in 4% buffered formalin for a maximum period of 24 hours. Fragments from the 2 groups were subjected to the following histochemical techniques; hematoxylin-eosin, Masson's trichrome and Picrosirius red for analysis of connective tissue, and immunohistochemical staining by using the anti-alpha-actin for the analysis of smooth muscle fibers and anti-elastin for analysis elastic system. Quantitative analysis was performed using Image J software with the differentiation method of color segmentation. RESULTS The analysis of sections stained by Picrosirius observed under polarized light showed no difference in collagen arrangement and distribution between treated and untreated patients. Both groups presented reddish homogeneous fibers in all analyzed fields, characterizing the presence of thicker fibers, possibly collagen type I. In the sections stained by Masson's trichrome, the quantitative analysis showed a significant decrease of approximately 34% of the collagen in individuals that used combo dart, when compared to controls. The immunohistochemical analysis showed an increase of 42% in the elastic system fibers in patients using combodart, when compared to controls. Regarding smooth muscle fibers, the patients in combodart group presented an increase of 81%. CONCLUSIONS Although these results are preliminary, with a small number of patients, to our knowledge, this is the first report of a comparative objective of BPH stromal components of controls and patients using combodart. The study would continue increasing the number of patients and an analysis of other components of the stroma and parenchyma. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e669 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Andre Cavalcanti More articles by this author Felipe Restrepo More articles by this author Waldemar Costa More articles by this author Carla Gallo More articles by this author Bruna Pereira More articles by this author Nathalia Machado More articles by this author Francisco Sampaio More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
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