Methanolic Leaf Research Articles

Haematology and histology studies on Moringa oleifera leaves and bark extracts in streptozotocin-induced diabetic albino rats

Alterations in haematological parameters are frequently observed in individuals with diabetes mellitus (DM). Oxidative stress has been identified as a contributing factor in the pathogenesis of organ damage and haematological dysfunction associated with DM. As a result, the potential positive impact of Moringa oleifera, a medicinal plant known for its antioxidant properties, on haematological parameters and kidney function in diabetic rats was investigated. A total of thirty-five rats were divided into seven groups, each consisting of five rats. Group I was administered only distilled water, while groups II to VII were induced with a single dose of 60mg/kg streptozotocin intraperitoneally. Subsequently, groups II to VI were treated with 150 mg/kg Moringa oleifera leaf methanol extract, 150 mg/kg Moringa oleifera bark methanol extract, 300 mg/kg Moringa oleifera leaf, 300 mg/kg Moringa oleifera bark, and metformin, respectively. The results showed positive modulation to most of the haematology parameters evaluated except neutrophils and lymphocytes by the treatments. The leaf extract showed a more significant modulation of platelets than other treatments. The histology of the kidney showed normal structure for all the treatments. Moringa oleifera especially the leaf extract is promising in the treatment of DM

Evaluation of antioxidant, anti-inflammatory and cytotoxic potential of phytochemicals from Gymnostachyum febrifugum-an in vitro study

Gymnostachyum febrifugum an ethnomedicinal plant of Acanthaceae family is used to treat fever, ulcer and metrorrhagia. The current study aimed to determine the antioxidant, anti-inflammatory and cytotoxic potential of phytochemicals from methanolic leaf and root extracts of G. febrifugum. In addition, a preliminary phytochemical analysis was carried out using thin layer chromatography (TLC) and liquid chromatography-mass spectrometry (LC-MS). The study revealed significant antioxidant and anti-inflammatory activities of methanolic extracts of G. febrifugum roots compared to that of leaves. Also, a moderate in vitro cytotoxic effect of the root methanolic extracts was observed against human lung cancer cells. LC-MS analysis of the methanolic extract of root revealed the presence of 11 phytochemicals belonging to classes of alkaloids, flavonoids, terpenoids and sugars, of which esculin, 6,7,10-trihydroxy-8-octadecenoic acid, piperlongumine, ardisiacrispin B and berbamine were detected in high abundance. Probably, these phytochemicals were responsible for the antioxidant, anti-inflammatory and cytotoxic potential of G. febrifugum. However further research on isolation and identification of active compounds is necessary to confirm these results.

Eco-friendly synthesis of silver nanoparticles using Phyllanthus niruri leaf extract: Assessment of antimicrobial activity, effectiveness on tropical neglected mosquito vector control, and biocompatibility using a fibroblast cell line model

Abstract Mosquitoes are rapidly advancing as vectors of several severe diseases. The increasing resistance of mosquitoes and the environmental harm caused by insecticides pose significant challenges for eradicating mosquito vectors. In this study, 18 plant extracts were tested for larvicidal properties against Aedes aegypti, Culex quinquefasciatus, and Anopheles stephensi larvae. Phyllanthus niruri (Pn) showed enhanced larvicidal activity in both laboratory and field trials. The biosynthesis of silver nanoparticles (AgNPs) using Pn leaf methanol extract (Pn-LME) was confirmed by UV-visible spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and Fourier-transform infrared spectroscopy. Among various concentrations, 3 mM AgNPs exhibited significant LC90 values of 0.83, 1.46, and 9.11 ppm compared to 9.25, 93.48, and 14.60 ppm of Pn-LME against A. stephensi, C. quinquefasciatus, and A. aegypti, respectively. This indicates the high mortality of mosquito vectors at low AgNP concentrations. Additionally, Pn-AgNPs showed enhanced antibacterial activity and no cytotoxicity in normal fibroblast cells (L929). Field trials demonstrated a 98.70% decrease in mosquito larval density at A. stephensi breeding sites, a 96.55% reduction at C. quinquefasciatus sites, and a 97.85% reduction at Ae. aegypti sites. This study presents an eco-friendly and cost-effective AgNP bio-pesticide synthesized from Pn leaves for controlling and preventing the transmission of filarial, dengue, and malaria vectors.

Unraveling the Ferroptosis-inducing Potential of Methanol Leaves Extract of Prosopis Juliflora Via Downregulation of SLC7A11 and GPX4 mRNA Expression in A549 Lung Cancer Cells.

Prosopis juliflora has been employed in many traditional treatments. As evidenced by our earlier research, Prosopis juliflora leaf methanol extract (PJME) has a promising future in the fight against lung cancer. It may also be used in conjunction with other treatments to effectively manage lung cancer. The main objective of this study was to explore the potential of PJME to inhibit lung cancer in A549 cells, along with its underlying mechanisms of action. The antiproliferative effects were determined using MTT and LDH tests. Apoptosis- inducing capacity was evaluated using the DAPI staining, caspase-3 test, cytochrome C assay, PARP cleavage, and qRT-PCR. To investigate the mechanism of action of PJME in lung cancer, the levels of ROS, MMP, GSH, MDA, and specific ferroptosis indicators were measured. The experimental data of the current study indicated that exposure of A549 cells to PJME reduced cell viability and increased cellular cytotoxicity. The apoptosis-inducing ability of PJME in A549 cells was validated by enhanced nuclear condensation, level of the caspase- 3, cytochrome C, and PARP release. In addition, qRT-PCR investigations verified that the administration of PJME led to a decrease in the expression of anti-apoptotic gene Bcl2 while enhancing the mRNA level of pro-apoptotic genes, such as Bax and caspase-3, in A549 cells. The study also found that PJME has the ability to activate ferroptosis pathways, as evidenced by elevated reactive oxygen species (ROS) generation, changes in the levels of antioxidant markers (MDA and GSH), and decreased expression of SLC7A11 and GPX4. The results of the present study clearly showed that PJME inhibited the proliferation of A549 cells and induced ferroptosis by reducing the expression of the important targets SLC7A11 and GPX4. Further research is necessary to fully understand the clinical efficacy of PJME before it can be investigated as supplemental or adjuvant therapy for lung cancer.

Crop Improvement of Moringa oleifera L. through Genotype Screening for the Development of Clonal Propagation Techniques of High-Yielding Clones in Malaysia.

Moringa oleifera L. is a valuable multipurpose tree species widely planted for centuries due to its high medicinal value and antifungal, antiviral, antidepressant, and anti-inflammatory properties in the food industry. However, its cultivation is hindered by production constraints such as the unavailability of planting material and the inadequate number of high-yielding clones. Thus, a study was initiated to select high-yielding clones in terms of growth and chemical content for the mass propagation of superior moringa trees. Screening on high-yielding clones with high astragalin content was conducted through the high-performance liquid chromatography (HPLC) analysis of moringa leaf extract. Selected genotypes were evaluated for their anti-inflammatory potential through in vitro bioactivity assays of leaf methanol extract. The effects of the rooting hormone, rooting substrates, and size of the cutting on the rooting response of branch cuttings of moringa were investigated. Results found that samples collected from different ecological zones of Peninsular Malaysia show significant variation in terms of astragalin content. The extracts were observed to show considerable variation in biological activity against the pro-inflammatory enzymes. The size of the cuttings had significant effects on the rooting of the cuttings as longer cuttings with bigger diameters rooted better than shorter cuttings with smaller diameters. Several genotypes of M. oleifera with superior phenotypic characteristics and bioactive compounds have been identified. Factors affecting the rooting efficiency and optimal conditions of rooting are suggested, which provides valuable information for the propagation of the superior planting material of moringa. This effort will ensure the sustainable production and supply of good quality raw materials for the production of quality end-products for the food and pharmaceutical industry.

Tyrosinase Inhibitory Activity of The Extracts from Garcinia lateriflora Blume var. Javanica Boerl, Garcinia fruticosa Lauterb, and Garcinia xanthochymus

Tyrosinase overexpression in humans increases melanin production in the skin, which may have hyperpigmentation-causing effects. In this study, various extracts from the Garcinia lateriflora Blume var. Javanica Boerl, Garcinia fruticosa Lauterb, and Garcinia xanthochymus plants were tested for their ability to inhibit the tyrosinase enzyme. The results showed that the G. fruticosa Lauterb stem bark hexane extract had the highest activity followed succesively by the G. lateriflora Blume var javanica Boerl leaf methanol extract, G xanthochymus mesocarp hexane extract, G. xanthochymus mesocarp methanol extract. The results show that the G. fruticosa Lauterb stem bark hexane extract has the highest tyrosinase inhibitory activity (45.61 ± 1.58%) among the four extracts and the tyrosinase inhibitory activity of kojic acid (65.07 0.86%).

Phytochemical composition of <i>Eriobotrya japonica</i> (Rosaceae) leaves extracts from central Veracruz, Mexico, and its effect on α-glucosidase enzyme inhibition

Background: Eriobotrya japonica is economically significant as an ornamental tree, and its leaves have medicinal properties. Question: What are the main chemical components of loquat leaves grown in a population of Central Veracruz Mexico? and does the methanolic extract have potential antidiabetic properties through α-glucosidase inhibition? Species study: Eriobotrya japonica (Thunb.) Lindl. (Rosaceae) Study site and date: Xalapa, Veracruz, Mexico, 2021-2022. Methods: Total carbon (C) and nitrogen (N) content, phosphorus (P), sodium (Na), potassium (K), calcium (Ca), and magnesium (Mg) were determined in leaves powder. Leaf methanol extract of E. japonica was tested for α-glucosidase inhibition. Also, different groups of secondary metabolites were detected by the phenolics/volatile-targeted metabolomic analysis. Results: The leaves of E. japonica are rich in C and minerals such as Na, K, Ca, and Mg, and contain high levels of flavonoids, and procyanidin B2. The leaf methanol extract (LME) effectively inhibited α-glucosidase activity (86.05 ± 0.73 %) in vitro. In addition, a leaf petroleum ether extract (LPE) contains mainly phytol, palmitic acid, linoleic acid, stearic acid, and phytol acetate. Conclusions: The leaf methanolic extract exhibited antidiabetic potential due to its potent α-glucosidase inhibition, and the presence of diverse phenolic compounds, including flavonoids and their glycoside derivatives, and some fatty acids further supports the traditional use of E. japonica as an herbal medicine with antidiabetic properties.

Nutritional, phytochemistry, antioxidant, and antidiabetic potentials of Hippocratea velutina (Afzel.) leaves: In vitro, ex vivo and in silico studies

BackgroundHippocratea velutina is a novel folk plant that is used for lowering blood glucose and is hence a potential source of new antidiabetic medication. Therefore, this study was designed to investigate the antidiabetic and antidiabetic potential of the methanol extract of Hippocratea velutina leaves. MethodsThe nutritional, elemental, and phytochemical properties of H. velutina leaves were investigated. 1,1-diphenyl-2-picrylhydrazine (DPPH) scavenging ability, Fe2+chelation and nitric oxide scavenging abilities, FeSO4-induced pancreatic injury parameters such as catalase, GSH, ENTPase, Na/K ATPase activities, α-glucosidase and α-amylase inhibitory activities were used to assess the antioxidant and antidiabetic properties of H. velutina leaf. Furthermore, HPLC-identified constituents of H. velutina leaf methanol extract were docked against human α-glucosidase (3TOP) and human α-amylase (1B2Y). ResultsThe results revealed that H. velutina contains protein, crude fat, flavonoids, anthraquinones, magnesium, sodium, and iron. Compared with quercetin, H. velutina significantly scavenged NO• radicals and chelated Fe2+. H. velutina exhibited a greater inhibitory effect on FeSO4-induced pancreatic injury. Furthermore, H. velutina showed a dose-dependent increase in the activity of ENTPDase and the Na/K ATPase enzyme, and it also had a significantly greater inhibitory effect on α-amylase and α-glucosidase enzymes than metformin. Computational study revealed that lutein showed the highest binding free energy to 3TOP, while apigenin offered the highest binding free energy to 1B2Y. ConclusionHippocratea velutina leaf possesses significant antioxidant and antidiabetic activities.

Mikania micrantha Kunth leaf Methanol Extract as Bioherbicide Againts The Growth of Amaranthus spinosus L. Weeds

The presence of weeds on plantation land causing a decrease in growth and productivity of cultivated plants. Thorny amaranth (Amaranthus spinosus L.) is broadleaf weeds that have high competitiveness. This study aims to determine the influence of the application of bitter vine (Mikania micrantha Kunth) leaf extracts against the growth of thorny amaranth and the best concentration that can inhibit the growth of thorny amaranth weed. This study has been carried out for October 2023 - March 2024 in the Biology Screen House and Chemistry Laboratory, Faculty of Mathematics and Natural Sciences, Tanjungpura University. This study used a completely randomized design (CRD) with four levels concentration treatment of bitter vine leaf extracts with five replications. The treatment consisted of bitter vine leaf extract with a concentration 0% (0g/ml) as a control, 15% (0,15 g/ml), 20% (0,2 g/ml) and 25% (0,25 g/ml). Data was analysed using Kruskal Wallis test followed by Mann-Whitney test. The results showed giving bitter vine leaf extracts can inhibited the growth of thorny amaranth weeds. Application of bitter vine leaf extract concentration 15% was the best treatment that can inhibited the growth of thorny amaranth on parameters weeds height (2,53 cm), number of leaves (2,65 strands), root length (1,74 cm), wet weight (0,13 grams) and dry weight (0,01 grams).

The Potential Alternative Antibacterial Activity of Falcata (Falcataria Falcata) Leaf Methanolic Extract against Staphylococcus Aureus and Escherichia Coli

The Potential Alternative Antibacterial Activity Of Falcata (Falcataria falcata) Leaf Methanolic Extract Against Staphylococcus aureus And Escherichia coli  Laliefe B. Arnan; Katherine Joy S. Escueta; Nor Rashida P. Rashid; Elyca M. Tacbobo; Queenie Rose M. Tinoy  Degree: Bachelor of Science in Pharmacy  Thesis Adviser: Junnin Gay L. Garay, RPh, CPh, MS Pharm Falcata is a plant that can be found in the Philippines and is used for the production of wood veneer and plywood. While in Indonesia, it is used as traditional remedy for malaria (Budiarti et al. 2020). They belong to the Fabaceae family, a family known for having great antibacterial effects (Gamo et al. 2015). This study used a percolation extraction method and the percentage yield is calculated to determine the yield from the falcata extract. Disc diffusion method is used for susceptibility testing and determining the zone of inhibition for the different groups. The CLSI guidelines for Staphylococcus aureus and Escherichia coli will be used to determine the antibacterial effect of the extract, in terms of resistance, intermediate, and susceptible results. From the results, the percentage yield of the methanolic crude leaf extract of Falcataria falcata is 2.67%. Leaves from the Falcataria falcata plant were extracted and tested against bacteria. The extracts showed promise in inhibiting the growth of Staphylococcus aureus and Escherichia coli bacteria, with 75% concentration as being more effective. However, these bacteria showed some resistance to all extract concentrations: S. aureus (90% - resistant, 75% - intermediate, 50% - resistant, 25% - resistant); E. coli (90% - resistant, 75% - resistant, 50% - resistant, 25% - resistant). Further study is needed to determine the exact antibacterial properties of the plant.

UJI KONSENTRASI EKSTRAK DAUN SIRIH HUTAN BERPELARUT ORGANIK TERHADAP WERENG COKLAT

Rice is one of the priority food crop in Indonesia. The main problem faced by rice cultivation is the attack of brown planthopper (Nilaparvata lugens Stall.), this pest is one of the most important pest in rice cultivation, because it can cause a production decrease up to 100% by causing hopperburn symptoms and becoma a desease vector. Botanical pesticide of spiked pepper (Piper aduncum L.) can be the safe alternative to controling brown planthopper. This research aims to obtain effective concentration to controling brown planthopper. The research was conducted at The Plant Pest Laboratory Faculty of Agriculture and Organic Chemistry and Natural Materials Laboratory Faculty of Engineering, Riau University, from November 2020 to Januari 2021, using completely randomized design (CRD) consist of 6 treatments 4 replications. The treatment application consisted of concentrations of 0%, 0.20%, 0.40%, 0.60%, 0.80%, and 100% spiked pepper leaf methanol solvent extract. The result showed that different concentration significantly affected to the death of brown planthopper. Concentrations starting from 0.20% has good performance in killing pest, but the effective concentration to control brown planthoppers is 0.60% concentration of spiked pepper leaf methanol extract, with a total mortality of 82.50%, initial time of death 3.25 hours, and 50% time of death at 10 hours after application.

Comparison of Antioxidant Activity of Methanol Extract of Young and Old Leaves of Avocado (Persea americana Mill.)

Avocado plants (Persea americana Mill.) are widely grown in tropical and subtropical regions. Avocado leaves contain natural antioxidants for traditional medicine. This study aims to determine the total phenolic content, total flavonoids, and antioxidant activity values of young and old avocado leaves, and analyze the relationship between total phenol content and total flavonoids to the antioxidant activity value of avocado leaf methanol extract (Persea americana Mill.). The macerated avocado leaf extract was quantitatively tested for antioxidant activity using the DPPH method and the total phenolic and flavonoid contents were calculated using the Follin-Ciocalteu and Follin-Ciocalteu methods, respectively.The results showed that the highest total phenolic content of old avocado leaves was 1.682,27mg GAE/100g dry weight. The highest total flavonoid content of avocado old leaves was 3.858,96mg QE/100g dry weight. Antioxidant activity of methanol extract of young avocado leaves has a value of IC50 321.32 ± 80.43 ppm and avocado old leaves amounted to 288.54 ± 33.06 ppm. Total flavonoid content was more influential on antioxidant activity, with a correlation coefficient of 0.856

In-vitro Anti-bacterial Activity of Methanol and Aqueous Crude Extracts of Horsfieldia iryaghedhi

Aims: Over the past two decades, the rise of multidrug resistance (MDR) in bacteria has posed a significant threat to global health. The urgent need for new treatment alternatives has brought attention to the potential of plants, which harbor a wealth of unexplored phytochemicals with therapeutic properties. This study aims to evaluate the anti-bacterial efficacy of methanol and aqueous extracts from the leaves and bark of Horsfieldia iryaghedhi In vitro. Methodology: Aqueous and methanol extracts were obtained from the cold maceration method. In vitro anti-bacterial activity of methanol and aqueous leaf, bark, and combination extracts were determined against gram-negative bacteria Escherichia coli (ATCC® 25922) and gram-positive bacteria Staphylococcus aureus (ATCC® 25923). The anti-bacterial assay for different concentrations of each extract was conducted through the well-diffusion method, with Gentamycin serving as the positive control. Results: Methanol leaf and combination extracts of Horsfieldia iryaghedhi have shown a positive anti-bacterial response at their highest concentrations of 1000µg/mL and 500µg/mL against gram-positive bacteria Staphylococcus aureus while none of the extracts showed anti-bacterial activity against gram-negative E. coli at the experimented concentrations. Conclusion: The study concludes that methanol extracts of H.iryaghedhi should be further analyzed for their anti-bacterial activity, and there could be potential lead molecules that can be developed as antibiotics.

Tinospora malabarica leaf extract fractions-derived silver nanoparticles and their spectral characterisation: evaluation of their toxicity properties against agricultural pests Spodoptera litura and Helicoverpa armigera

ABSTRACT Our study specifically delves into the synthesis of AgNPs utilizing Tinospora malabarica leaf extract fractions (Tm-LMEFs) and explore their efficacy in combating the larvae have progressed to the synthesized AgNPs (Tm-AgNPS) and the leaf methanol extract fractions (LMEFs), our investigation focused on their impact on the targeted larvae species over a 24-h exposure period. Significantly noteworthy mortality rates were observed across all Spodoptera litura and Helicoverpa armigera post-treatment, indicating pronounced efficacy. Notably, the Tm-AgNPS exhibited higher toxicity compared to the LMEFs these selected polyphagous insect pests. Quantitative analysis yielded the highest mortality (98.99%/90.44% and 55.33%/49.60%) values for the synthesized Tm-AgNPs against S. litura and H. armigera, with no observed mortality in the control group. This study marks the first instance of exploring both Tm-LMEFs, Tm-AgNPs and enzyme activity have toxicity properties against selected agriculture pests, highlighting their promising role in pest management and environmental sustainability.

Attenuation of Cadmium Toxicity by Methanol Extracts of Rauvolfia vomitoria and Aframomum melegueta leaves

Highlights:1. This study provides insight into the detrimental effect of cadmium exposure on mammalian cells using a murine model.2. The co-administration of Rauvolfia vomitoria and Aframomum melegueta leaf extracts is more effective in ameliorating cadmium-induced toxicity than a single administration of each plant extract.3. The co-administration of Rauvolfia vomitoria and Aframomum melegueta plant extracts can upregulate the expression of IL-10 and reverse the derangements in the TNF-α, IL-6, SOD, and GPX levels. Abstract Cadmium has long been known to be an environmental pollutant that can harm human health, with toxicity majorly affecting the kidneys and liver. This study aimed to investigate the mitigating potential of methanol extracts of Rauvolfia vomitoria and Aframomum melegueta leaves in cadmium-induced liver toxicity. Twenty-five male adult Wistar rats, averagely weighing 200 g, were randomly allocated into five groups, each comprising five rats. Group 1 was unexposed to any substances and only received distilled water. Group 2 was given cadmium at a standard dose of 12 mg/kg bw. Groups 3, 4, and 5 received 12 mg/kg bw of cadmium, and each group was treated with 200 mg/kg bw of Rauvolfia vomitoria leaf extract, Aframomum melegueta leaf extract, and a combination of both extracts, respectively. After 28 days, the animals were euthanized to obtain their livers, which were then excised and processed for histopathological, mRNA expression, and biochemical analyses. A one-way analysis of variance (ANOVA) was used to analyze the data, and Duncan multiple tests were employed to compare the categorical variables (p<0.05). The results revealed elevated levels of interleukin 10 (IL-10) in the rats treated with Rauvolfia vomitoria and Aframomum melegueta extracts when compared to Group 2. On the other hand, the treatment groups exhibited a significant decrease in tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) levels, along with significantly elevated levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx). Group 5 showed a normal liver histoarchitecture similar to Group 1, reversing the histopathological abnormalities shown in Group 2. In conclusion, the co-administrative treatment using Rauvolfia vomitoria and Aframomum melegueta extracts reversed the cadmium-induced toxicity better than using each plant extract individually. This further suggests that Rauvolfia vomitoria and Aframomum melegueta leaf methanol extracts may ameliorate the effects of cadmium-induced toxicity, including reducing oxidative stress and fortifying the anti-oxidant system.

Phytochemical constituents and antioxidant activity of Ricinus communis Linn leaf and seeds extracts

The study investigates phytochemicals and antioxidant potential of leaf and seeds extracts of Ricinus communis L. Ricinus communis L. commonly known as castor plant belongs to the family Euphorbiacea. Phytochemical screening showed that both seeds and leaves of Ricinus communis L. have various secondary metabolites such as: tannins, flavonoids, alkaloids, triterpenes and coumarins, while anthraquinones were not detected. Antioxidant potentials were assessed on the basis of radical scavenging effect of the stable 1,1-diphenyl-2-picrylhydrazil (DPPH). Leaf methanol Soxhlet extract showed the maximum antioxidant activity (85±0.01%) with IC50 value of (0.121±0.02mg/ml). Seed methanol maceration extract showed high antioxidant activity (80.7±0.01%) while petroleum ether extracts of both leaf and seeds showed no antioxidant activity. Spectroscopic analysis using GC-MS of the methanol maceration extract of leaf showed the presence of various compounds such as n-hexadecanoic acid with the highest peak area 22.02%, 4-methoxy-1-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile with peak area 17.41%, gamonelic acid with peak area 10.40%, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one with peak area 4.84% and 2-pyrrolidinone with peak area 3.98%.

Effect of heat use efficiency on bioproduction of drought resistance eliciting phytochemicals in groundnut (Arachishypogaea L.) genotypeGKVK-5

Thermal utilization factors are crucial throughout the crop period, from seedling emergence to maturity, significantly affecting crop yield. Groundnut (ArachishypogaeaL.) shows drought tolerance through secondary metabolites, helping maintain biomass during arid conditions. This study compared heat use efficiency with the HR-LCMS profiling of phytochemicals in groundnut genotype GKVK-5. A field study was conductedon sandy loam soils at AHRS, Bavikere, UAHS, Shivamogga, Karnataka, India, under rain-fed circumstances during the (Kharif)season. Results showed GKVK-5 had a total dry matter of over 18.75g per plant, with an increased HUE of 14.56g ºC day⁻¹ at 90 days after sowing. Higher HTUE (2.258 q ha⁻¹ HTU⁻¹) resulted in more pod output (16.73 q ha⁻¹) and haulm yield (26.99 q ha⁻¹) compared to TMV 2 (10.48 q ha⁻¹ and 15.07 q ha⁻¹). The increase in HUE demonstrates GKVK-5’s tolerance to drought stress. During a qualitative phytochemical screening, GKVK-5’s leaf methanol extract tested positive for alkaloids, flavonoids, and phenolics. The quantitative estimation yielded values of 32.44μg/mg for total alkaloids, 38.61μg/mg for flavonoids, and 47.32μg/mg for phenolics.HR-LCMS profiling identified drought resistance-eliciting phytochemicals, such as 2-Amino-3-methylhexanoic acid, 6-Deoxyfagomine Maltoxazine, and 2-Furanyl pyrrolidine, in cation mode. Antioxidant flavonoids Astragalin 7-rhamnoside, Naringenin 7-O-glucoside, and PhenolicsRutin, Gallic acid, and quinic acid were detected in anion mode using high DB hits, retention duration, and MS spectra. These oxidative stress molecules led to enhanced heat use efficiency and drought stress adaptation in genotype GKVK-5.

Phytochemical Profile and GC-MS-Identified Bioactive Compounds in Pluchea ovalis (Pers.) DC. Leaves

Background: Pluchea ovalis is a significant medicinal plant that belongs to the Asteraceae family. It is also known as “Woolly Camphor-Weed” and has traditionally been used in Ayurveda, Siddha, and Folk medicine for treating numerous ailments, particularly Jaundice, Piles, Bronchial Asthma, inflammation, and Carcinoma. Purpose: Phytochemical Profile and GC-MS-Identified Bioactive Compounds in Pluchea ovalis Research Design: The phytochemical composition of P. ovalis leaves was screened using a Soxhlet apparatus after hot, continuous, and consecutive extraction. A variety of solvents were used for the qualitative assay. Different solvents were used in increasing polarity order to carry out the extraction operation. Total content of tannins, phenolic compounds, alkaloids (bromocresol green), and flavonoids (aluminum chloride) quantified (Folin-Ciocalteu). Additionally, quick and precise GC was used in conjunction with the Mass Hunter tool and mass spectrometry method to examine the absorbed components and determine the complete elements. Results: The qualitative phytochemical screening revealed that alkaloids, flavonoids, saponins, steroids & terpenoids, phenols, tannins, glycosides, phytosterols, coumarins, quinones resins, cardiac glycosides, leuco anthocyanins, anthraquinones and fixed oils were found in all the extracts, the quantification of alkaloids in methanol, showcasing a remarkable concentration of 502 mg/g equivalent, while chloroform exhibits a substantial alkaloid content of 310 mg/g, flavonoids, yielding the highest concentration in methanol at 520 mg/g and ethyl acetate at 440 mg/g. Phenols and tannins in methanol 390 mg/g and 320 mg/g . Gas chromatography-mass spectrometry (GC-MS) analysis of P. ovalis leaf methanol extract revealed 50 peaks, identifying bioactive compounds based on retention time, molecular formula, structure, weight, and concentration (peak area%). The results indicated that the single major component, dodecanoic acid, 1,2,3-propanetriyl ester (C39H74O6), had a peak area of 38.16%, a molecular weight of 638, and was reported to have hypercholesterolemic, anti-arthritic, nematocidal and hepatoprotective activity. 2-Ethoxyethylamine (C4H11NO), on the other hand, had the second-highest peak area, at 26.46%

Antibacterial, Phytochemical and Toxicological Activities of Garcinia kola Extracts against Multidrug Resistant Clinical Bacteria

Objective: The rise in multiple antibiotic resistant microorganisms has led to a decline in the efficacy of many antibiotics, prompting the investigation of alternative solutions such as medicinal plants. To tackle this concerning issue, this study was undertaken to evaluate the phytochemical composition, antibacterial activity, and toxicological characteristics of Garcinia kola extracts against multidrug-resistant bacteria commonly found in clinical settings. Material-Method: The cold maceration technique was employed to extract the root and leaf of Garcinia kola using water and methanol. The extracts were then subjected to phytochemical screening. To evaluate the extracts' potential to inhibit bacterial growth, five multidrug-resistant isolates were utilized in this study. Agar well assay was used to determine the zones of inhibition. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using the broth macro dilution technique. Additionally, Brine shrimp lethality assay was conducted to determine the lethal concentration (LC50) of the extracts. The extracts were also combined for synergism. Results: The investigation found that the root extract in methanol had the highest yield at 25.58%. All four extracts contained ten out of the fourteen tested phytochemicals. The methanol leaf and root extracts exhibited the highest and lowest inhibition zones of 20mm and 9mm against Staphylococcus aureus, respectively. The MIC values ranged from 250 to 600 mg/ml, indicating broad-spectrum antibacterial activity. The cytotoxicity test showed a range of 33.03 to 126.3µg/mL for the aqueous and methanol extracts. Conclusion: Although Garcinia kola shows potential as a source of antibacterial compounds, caution should be exercised due to its toxic effects.

In vitro Antimicrobial Activity, Antioxidant Activity and Synergistic/Antagonistic Effect of Arum maculatum L. in Mersin Province, Turkey

Arum maculatum is used for the treatment of colitis and internal bleeding haemorrhoids among the local people in Turkey. This study was designed to evaluate the antioxidant and antimicrobial activity and protective effects of the leaf and root methanol extract of Arum maculatum L. The phenolic compound content of the root and leaf extract was determined as 207.135±0.07 µg GAE g-1, and 386.054±0.7 µg GAE g extract-1, respectively. The total flavonoid amount was determined as 53.386±0.220 µg QE g-1 and 347.704±0.352 µg QE g-1 extract, respectively. DPPH free radical scavenging of leaf and root extracts was determined as 76.69% and 67.33%, respectively.. The metal chelating effect was determined as 46.585±0.025 mg EDTA/g extract and 35.610±0.087 mg EDTA g extract-1 for roots and leaves, respectively. All extracts were effective against all tested bacteria, and Staphylococcus mutans ATTC 10449 was the most sensitive bacterium with the lowest MIC value (0.20 mg mL-1, 0.81 mg mL-1) for leaf and root extracts, respectively. The biggest diameter of growth inhibition zone (8±2.44 mm, 9±3.26) compared to Chloramphenicol. Arum leaf extracts showed a synergistic effect with OFX against Escherichia coli and Staphylococcus mutans, and Arum root extracts have a synergistic effect with OFX against Staphylococcus aureus and Staphylococcus mutans. These results displayed that A. maculatum has notable natural bioactive compounds with antioxidant and antibacterial properties.