Metal Ion Chelating Research Articles

Antioxidant and anti-advanced glycation end products formation properties of palmatine

Context: Oxidative stress and formation of advanced glycation end products (AGEs), due to glycation of proteins, lipids and nucleic acids are characteristic in diabetic patients. Palmatine, a protoberberine alkaloid bioactive isolated from Coscinium fenestratum (CF) stem extract, which previously has shown to possess antidiabetic and antioxidant properties and to be able to protect the kidney and liver in a STZ-diabetic induced rat model. Aims: To evaluate the in vitro and in vivo antioxidant and anti-AGE activity of palmatine. Methods: In vitro and in vivo studies were conducted to measure radical scavenging, reducing power, inhibition of lipid peroxidation, carbonyl trapping and metal ion chelation. In vitro antiglycation activity was done using bovine serum albumin-methylglyoxal (BSA-MGO), bovine serum albumin – glucose (BSA-GLU) and glycated hemoglobin. In vivo antioxidant and antiglycation activity were used to evaluate liver and kidney extracted from STZ-induced diabetic rat after treatment with palmatine. Results: The results showed palmatine blocked the formation of AGE as shown by the results of BSA-GLU, BSA-MGO, glycated hemoglobin and inhibited the free radicals generated by DPPH, nitric oxide, hydrogen peroxide, lipid peroxidation, FRAP and metal ion chelating. It was able to stimulate in vivo the activity of catalase, super oxide dismutase and glutathione peroxidase. Conclusions: Palmatine possess antioxidant and antiglycation properties, the mechanism of action seems to be via the blockage of free radical formation, decreasing of reactive carbonyl. Further research is ongoing to determine the effect of palmatine on glyoxalase 1 and aldose reductase pathway and interaction with receptor for AGE.

Development of a novel IMAC sorbent for the identification of melamine in dairy products by HPLC

To determine melamine (MEL) in dairy products, a novel immobilized metal affinity chromatography (IMAC) sorbent using chitosan (CTS) and 2-hydroxyethyl methacrylate (HEMA) as monomers, ethylene glycol dimethacrylate (EGDMA) as cross-linker and Cu2+ ions as chelated metal ion was synthesized by radical polymerization and characterized by Fourier transfer infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and swelling test. Adsorption parameters (pH, contact time, initial concentration, etc.) were studied to evaluate the adsorption process. The results showed that the CTS-p(HEMA)-Cu2+ IMAC sorbent displayed a rapid dynamic adsorption with a maximum adsorption capacity of 275.49 μg g−1 toward melamine. The CTS-p(HEMA)-Cu2+ IMAC sorbent was also applied to determine and enrich melamine in dairy products (milk and infant formula). An enrichment ratio of 5-fold, detection limit of 0.005 and 0.015 μg mL−1, and quantification limit of 0.0150 and 0.1261 μg mL−1 were achieved for MEL in milk and infant formula, respectively.

Nasal Delivery of D-Penicillamine Hydrogel Upregulates a Disintegrin and Metalloprotease 10 Expression via Melatonin Receptor 1 in Alzheimer's Disease Models.

Alzheimer’s disease (AD) is a type of neurodegenerative disease that is associated with the accumulation of amyloid plaques. Increasing non-amyloidogenic processing and/or manipulating amyloid precursor protein signaling could reduce AD amyloid pathology and cognitive impairment. D-penicillamine (D-Pen) is a water-soluble metal chelator and can reduce the aggregation of amyloid-β (Aβ) with metals in vitro. However, the potential mechanism of D-Pen for treating neurodegenerative disorders remains unexplored. In here, a novel type of chitosan-based hydrogel to carry D-Pen was designed and the D-Pen-CS/β-glycerophosphate hydrogel were characterized by scanning electron microscopy and HPLC. Behavior tests investigated the learning and memory levels of APP/PS1 mice treated through the D-Pen hydrogel nasal delivery. In vivo and in vitro findings showed that nasal delivery of D-Pen-CS/β-GP hydrogel had properly chelated metal ions that reduced Aβ deposition. Furthermore, D-Pen mainly regulated A disintegrin and metalloprotease 10 (ADAM10) expression via melatonin receptor 1 (MTNR1α) and the downstream PKA/ERK/CREB pathway. The present data demonstrated D-Pen significantly improved the cognitive ability of APP/PS1 mice and reduced Aβ generation through activating ADAM10 and accelerating non-amyloidogenic processing. Hence, these findings indicate the potential of D-Pen as a promising agent for treating AD.

Potentially Bioaccessible Phenolic and Antioxidant Potential of Fresh and Stored Lentil Sprouts—Effect of Lactobacillus plantarum 299v Enrichment

The phenolic and antioxidant potential of potentially bioaccessible fractions of lentil sprouts was studied. Sprouts were cocultivated with a probiotic to obtain a new functional product and further stored in cool conditions. The fraction obtained after buffer extraction and gastric digestion had higher content of phenolics compared to the control (by 20% and 46%, respectively); however, a 9% decrease was observed in samples obtained after gastrointestinal digestion. After gastrointestinal digestion, the highest content of phenolics (278 µg/g d.w.) was determined in the fresh control sprouts. Compounds neutralizing ABTS and hydroxyl radicals, chelating metal ions, and exhibiting strong reducing power were effectively released after gastrointestinal digestion (e.g., the values of the gastrointestinal digestibility index for chelating power and ability to quench hydroxyl radicals significantly exceeded 1 in all studied samples). It was proved that the enrichment of sprouts with a probiotic and further storage significantly improved the antioxidant potential; compared to the fresh control sprouts, an increase by 45% and 10% was determined after the gastric and gastrointestinal digestion, respectively. Lentil sprouts enriched with L. plantarum 299v may be a new functional product characterized by the high antioxidant capacity of the potentially bioaccessible fraction.

Selection of organic materials potentially used to enhance bioremediation of acid mine drainage

Acid mine drainage (AMD), produced when sulfide minerals are subjected to oxygen and water, is one of the major issues in mining industries. Without proper management, AMD's release to the environment would cause seriously prolonged environmental and health issues, such as increases soil acidity and reduces water quality due to extremely low pH, high sulphate concentration, and heavy metal solubility. AMD treatments are divided into two categories, i.e., active treatment, conducted by applying a chemical to the AMD to neutralize pH and precipitate heavy metals; and passive treatment, which relies on biological and biochemical processes. The active treatment may provide an immediate effect, but costly and yet sustainable; meanwhile, passive treatment takes time to establish and to generate an effect, but it is more economical, sustainable, and environmentally friendly. The wetland system is an example of passive treatment. Therefore, this review focuses on passive treatments, especially the selection of organic materials used in constructed AMD wetland treatment. Organic materials play a central role in the wetland system, i.e., to chelate metal ions, remove sulphate from the solution, increase pH, and growth media for microbes, especially sulphate reducing bacteria (SRB) and plants are grown in the system. Overall, organic materials determine the effectiveness of the wetland system to neutralize AMD passively and sustainably.

A Model System to Explore the Detection Limits of Antibody-Based Immuno-SPECT Imaging of Exclusively Intranuclear Epitopes.

Imaging of intranuclear epitopes using antibodies tagged to cell-penetrating peptides has great potential given its versatility, specificity, and sensitivity. However, this process is technically challenging because of the location of the target. Previous research has demonstrated a variety of intranuclear epitopes that can be targeted with antibody-based radioimmunoconjugates. Here, we developed a controlled-expression model of nucleus-localized green fluorescent protein (GFP) to interrogate the technical limitations of intranuclear SPECT using radioimmunoconjugates, notably the lower target-abundance detection threshold. Methods: We stably transfected the lung adenocarcinoma cell line H1299 with an enhanced GFP (EGFP)-tagged histone 2B (H2B) and generated 4 cell lines expressing increasing levels of GFP. EGFP levels were quantified using Western blot, flow cytometry, and enzyme-linked immunosorbent assay. An anti-GFP antibody (GFP-G1) was modified using dibenzocyclooctyne-N3-based strain-promoted azide-alkyne cycloaddition with the cell-penetrating peptide TAT (GRKKRRQRRRPPQGYG), which also includes a nuclear localization sequence, and the metal ion chelator N3-Bn-diethylenetriamine pentaacetate (DTPA) to allow radiolabeling with 111In. Cell uptake of 111In-GFP-G1-TAT was evaluated across 5 cell clones expressing different levels of H2B-EGFP invitro. Tumor uptake in xenograft-bearing mice was quantified to determine the smallest amount of target epitope that could be detected using 111In-GFP-G1-TAT. Results: We generated 4 H1299 cell clones expressing different levels of H2B-EGFP (0-1 million copies per cell, including wild-type H1299 cells). GFP-G1 monoclonal antibody was produced and purified in house, and selective binding to H2B-EGFP was confirmed. The affinity (dissociation constant) of GFP-G1 was determined as 9.1 ± 3.0 nM. GFP-G1 was conjugated to TAT and DTPA. 111In-GFP-G1-TAT uptake in H2B-EGFP-expressing cell clones correlated linearly with H2B-EGFP expression (P < 0.001). In vivo xenograft studies demonstrated that 111In-GFP-G1-TAT uptake in tumor tissue correlated linearly with expression of H2B-EGFP (P = 0.004) and suggested a lower target-abundance detection threshold of approximately 240,000 copies per cell. Conclusion: Here, we present a proof-of-concept demonstration that antibody-based imaging of intranuclear targets is capable both of detecting the presence of an epitope of interest with a copy number above 240,000 copies per cell and of determining differences in expression level above this threshold.

Cross-linking treatment of arabinoxylan improves its antioxidant and hypoglycemic activities after simulated in vitro digestion

The objective of the present study was to evaluate the impact of cross-linking treatment of arabinoxylan (AX) on its structure and physiological functions prior to and after the in vitro gastrointestinal digestion. The AX extracted from triticale bran was cross-linked using laccase. The reaction was monitored spectrophotometrically and verified based on the decrease in the ferulic acid content and an increase in the molecular weight. In comparison to the non-cross-linked AX (A-AX), the cross-linked AX (C-AX) exhibited a higher substitution degree, a more compact microstructure, and higher viscosity in the results of FT-IR, SEM, and rheological tests. The hydroxyl radical scavenging ability and the metal ion chelating ability of C-AX were higher than those of A-AX. After passing through the gastrointestinal tract simulated using an in vitro digestion model, in addition to the retentive larger molecular weight and network structure, the antioxidant activity and the glucosidase inhibition activity of C-AX were higher than those of A-AX. In conclusion, the cross-linking treatment of AX increased its tolerance of the extreme environment during the digestive process by modulating its structural characteristics.

High‐pressure microfluidization of sugarcane juice: Effect on total phenols, total flavonoids, antioxidant activity, and microbiological quality

Sugarcane juice is rich in bioactive compounds but is easily degraded during processing. Here, we observe the effect of microfluidization, an emerging high-pressure technique, on the phenols, flavonoids, antioxidants (by four assays), and microbiological safety of sugarcane juice. Results show a reduction of 30%−58% in phenols, 14%−54% in flavonoids, 24%−65% in ferric reducing antioxidant power, and 13%−64% in metal ion chelating activity of sugarcane juice. 2,2-diphenyl-1picryl hydrazil (DPPH) radical scavenging activity was unaffected after microfluidization, while hydroxyl radical scavenging activity increased by 31%−33% up to 100 MPa pressure. Sequential color palette analysis highlighted 100–150 MPa with three processing cycles as a suitable microfluidization conditions for sugarcane juice. However, sample processed at 150 MPa/3 cycles exhibited higher polyphenols and antioxidants in addition to being microbiologically safe. Variation in sugarcane juice parameters with microfluidization was found different from that of other food processing techniques perhaps due to its unique principle of operation. Practical applications Bioactive components of sugarcane juice are very sensitive to processing and require adequate food processing interventions for retention. Thermal and certain nonthermal methods of processing are known to degrade the polyphenols of sugarcane juice thereby depleting its nutritional quality. Due to these constraints and the unavailability of suitable processing technique/condition, the commercialization of sugarcane juice becomes difficult. This work investigates an emerging nonthermal technique and its effect on sugarcane juice polyphenols and antioxidants. The efficiency of microfluidization in retention of these polyphenols has been quantified for potential industrial application of the technique for plant based extracts.

Expression of SSEA-4 and Oct-4 from somatic cells in primary mouse gastric cell culture induced by brief strong acid.

Environmental changes can stress and alter biology at the molecular and cellular level. For example, metal-protein interaction is a classic physic and biological property of nature, which is fundamentally influenced by acidity. Here, we report a unique cellular reprogramming phenomenon in that a brief strong acid treatment induced the expression of pluripotent stem cell (PSC) markers. We used strong acid to briefly challenge mix-cultured gastric cells, and then subcultured survived cells in anormal cell culture medium. We found that survival acid-treated cells expressed PSC markers detected by commonly used pluripotent antibodies such as SSEA-4 and Oct4. In addition, we observed that the survived cells from theacid challenge grew faster during the second and third weeks of subculture and hada relative short doubling time (DT) than the controls. PSC marker-labeled 'older' cells also presented immature cell-like morphology with some having marker Oct4 in the nucleus. Finally, the expression of the markers appeared to be sensitive to metal ion chelation. Removal of the metals during a brief acid treatment reduced pluripotent marker-positive cells, suggesting the dissociation of metals from metal-binding proteins may be a factor involved in the induction of stem cell markers. Our findings reveal that somatic cells appear to possess a plasticity feature to express pluripotent marker proteins or to select cell subpopulations that express pluripotent marker proteins when cells are transiently exposed to strong acid. It opens new directions for understanding conserved regulatory mechanisms involved in cellular survival under stressful stimulation.

Characterization of phytochemicals and validation of antioxidant and anticancer activity in some Indian polyherbal ayurvedic products

In the present comparative study, the authors studied the antioxidant and anticancer activity of commercially available polyherbal Indian Ayurvedic products namely Divya Sarvakalp Kwath (DSKK), Divya Sanjivani Vati (DSV), Kanchanar Guggulu (KG) and Shakti Drop (SD). Authors also quantified phenolic and flavonoid contents in the samples. Solid powdered samples (DSKK, DSV, and KG) were extracted in methanol and water (1:1) using cold extraction method. Spectrophotometry technique was used to quantify the phytochemicals present in test samples. DSKK showed comparatively higher content of total phenolics (247.65 ± 0.05 μgPGE/g) and flavonoid (34.66 ± 0.19 µgQE/mg). Radical scavenging, metal ion chelation and reducing potential of test products were studied using nitric oxide scavenging, DPPH, metal ion chelation, reducing power ability, and phosphomolybdate in vitro antioxidant assays at different concentration. Dose-dependent antioxidant activity was observed in all the test samples at 100–500 µg or µl/ml concentration. Anticancer efficacy of the test samples were studied in lung (A549), colon (Colo205), and breast cancer (MCF7) cell lines at different concentrations (10–100 µg or µl/ml) using MTT assay. Confocal microscopy was used to reveal the apoptotic induction, mitochondrial membrane integrity disruption and reactive oxygen species production ability of test products in cancer cells. The present study revealed that DSKK possesses comparatively better antioxidant potential and SD has potent anticancer activity against breast cancer cells.

Amyloid-like aggregates of bovine serum albumin for extraction of gold from ores and electronic waste

Abstract Extracting gold resource from ore and electronic equipment is an important research topic worldwide, because gold has great value in various fields. The existing technology has the disadvantages of high energy consumption, difficult recycling, high cost and poor selectivity. Facing of these challenges, this work underlines the function of amyloid-like protein aggregates as a competitive biological adsorbent for recovering precious metals from low-grade ores and electronic waste. Through the triggering of the rapid amyloid-like aggregation of bovine serum albumin (BSA) via the reduction of its intramolecular disulfide bond, a sandwich-like phase-transited BSA (PTB) membrane is obtained in aqueous solution. The resultant material exhibits gold adsorption capacity per unit area up to ~316 mg g−1 m−2, which is ~2100 times higher than those of commonly used industrial adsorbents, such as activated carbon and resins. The material cost of the membrane is 1/12 that of the resin and close to that of activated carbon, and the material also shows advantageous adsorption capacities towards other precious metals, such as Ag, Pd, Pt and Ir. Electrostatic interactions, metal ion chelation and reduction on PTB are the main adsorption mechanisms of this protein-based material. Without any reducing agent, this material can directly reduce adsorbed gold ions to produce gold with a purity of 22 K after pyrolysis. This work underlines a design principle towards cost-effective high-performance biosorbent by manipulating amyloid-like aggregation of proteins to facilitate the exposure of functional groups, which is promising to reduce secondary pollution and meet the targets of circular economy and bioresource reutilization.

Do Sulfonamides Interact with Aromatic Rings?

Aromatic rings form energetically favorable interactions with many polar groups in chemical and biological systems. Recent molecular studies have shown that sulfonamides can chelate metal ions and form hydrogen bonds, however, it is presently not established whether the polar sulfonamide functionality also interacts with aromatic rings. Here, synthetic, spectroscopic, structural, and quantum chemical analyses on 2,6-diarylbenzenesulfonamides are reported, in which two flanking aromatic rings are positioned close to the central sulfonamide moiety. Fine-tuning the aromatic character by substituents on the flanking rings leads to linear trends in acidity and proton affinity of sulfonamides. This physical-organic chemistry study demonstrates that aromatic rings have a capacity to stabilize sulfonamides via through-space NH-π interactions. These results have implications in rational drug design targeting electron-rich aromatic rings in proteins.

Synthesis and Bio-Evaluation of N-Benzylpiperidine-8-Hydroxyquinoline Derivatives as Potential Cholinesterase Inhibitors, Metal Ion Chelators and Calcium Channel Blockers

Synthesis and Bio-Evaluation of N-Benzylpiperidine-8-Hydroxyquinoline Derivatives as Potential Cholinesterase Inhibitors, Metal Ion Chelators and Calcium Channel Blockers

ROLE OF NON-PROTEIN AMINO ACIDS IN AUTOIMMUNE DISEASES

Many nonprotein amino acids (NPAAs) are plant secondary metabolites and have a similar chemical structure , size, shape and charge to protein amino acids and can be mistakenly used in protein synthesis, interfere in biochemical pathways, overstimulate receptors or chelate metal ions. The Consumption of a number of plants that contain NPAAs has been shown to have acutely toxic effects in humans. The consumption of some seeds or vegetables that contain NPAAs inuences some people as a trigger for some diseases or as an exacerbating factor. The effects of NPAAs on human health are not well understood. It has been observed that chimpanzees fed alfalfa and also a volunteer subject who have intake alfalfa tablets have both developed a disease with all the characteristics of lupus erythematosus ( an autoimmune disease) and by suppressing the consumption of alfalfa the disease disappears. Most of NPAAs are of plant origin; these do not form part of the primary structure of proteins, they act as antimetabolites and most are found in higher plants; they have the same basic structure as amino acids of animal origin. The substitution of a protein amino acid in protein synthesis produces abnormal proteins which generate antibodies and modication of autoantigens as mechanism to break immunological tolerance and trigger autoimmunity. Auto-antibodies formation is a characteristic of autoimmune disease. The toxic potential of NPAAs depend the amount ingested and the consumption time. Chronic exposure to NPAAs could contribute to autoimmune disease development in genetically susceptible individuals

Metal-Ion Chelating Gel Polymer Electrolyte for Ni-Rich Layered Cathode Materials at a High Voltage and an Elevated Temperature.

Nickel-rich layered oxides (LiNi1-x-yCoxMnyO2; (1 - x - y) ≥ 0.6), the high-energy-density cathode materials of lithium-ion batteries (LIBs), are seriously unstable at voltages higher than 4.5 V versus Li/Li+ and temperatures higher than 50 °C. Herein, we demonstrated that the failure mechanism of a nickel-rich layered oxide (LiNi0.6Co0.2Mn0.2O2) behind the instability was successfully suppressed by employing cyanoethyl poly(vinyl alcohol) having pyrrolidone moieties (Pyrd-PVA-CN) as a metal-ion-chelating gel polymer electrolyte (GPE). The metal-ion-chelating GPE blocked the plating of transition-metal ions dissolved from the cathode by capturing the ions (anode protection). High-concentration metal-ion environments developed around the cathode surface by the GPE suppressed the irreversible phase transition of the cathode material from the layered structure to the rock-salt structure (cathode protection). Resultantly, the capacity retention was significantly improved at a high voltage and a high temperature. Capacity retention and coulombic efficiency of a full-cell configuration of a nickel-rich layered oxide with graphite were significantly improved in the presence of the GPE especially at a high cutoff voltage (4.4 V) and an elevated temperature (55 °C).

Neuropeptides: Roles and Activities as Metal Chelators in Neurodegenerative Diseases.

Neurodegenerative diseases, such as Alzheimer’s disease (AD) and Parkinson’s disease (PD), are characterized by deposits of amyloid proteins. The homeostasis of metal ions is crucial for the normal biological functions in the brain. However, in AD and PD, the imbalance of metal ions leads to formation of amyloid deposits. In the past four decades, there has been extensive effort to design compound agents than can chelate metal ions with the aim of preventing the formation of the amyloid deposits. Unfortunately, the compounds to date that were designed were not successful candidates to be used in clinical trials. Neuropeptides are small molecules that are produced and released by neurons. It has been shown that neuropeptides have neuroprotective effects in the brain and reduce the formation of amyloid deposits. This Review Article is focused on the function of neuropeptides as metal chelators. Experimental and computational studies demonstrated that neuropeptides could bind metal ions, such as Cu2+ and Zn2+. This Review Article provides perspectives and initiates future studies to investigate the role of neuropeptides as metal chelators in neurodegenerative diseases.

Benzothiazolyl and Benzoxazolyl Hydrazones Function as Zinc Metallochaperones to Reactivate Mutant p53.

We identified a set of thiosemicarbazone (TSC) metal ion chelators that reactivate specific zinc-deficient p53 mutants using a mechanism called zinc metallochaperones (ZMCs) that restore zinc binding by shuttling zinc into cells. We defined biophysical and cellular assays necessary for structure-activity relationship studies using this mechanism. We investigated an alternative class of zinc scaffolds that differ from TSCs by substitution of the thiocarbamoyl moiety with benzothiazolyl, benzoxazolyl, and benzimidazolyl hydrazones. Members of this series bound zinc with similar affinity and functioned to reactivate mutant p53 comparable to the TSCs. Acute toxicity and efficacy assays in rodents demonstrated C1 to be significantly less toxic than the TSCs while demonstrating equivalent growth inhibition. We identified C85 as a ZMC with diminished copper binding that functions as a chemotherapy and radiation sensitizer. We conclude that the benzothiazolyl, benzoxazolyl, and benzimidazolyl hydrazones can function as ZMCs to reactivate mutant p53 in vitro and in vivo.

Further insight on amine-metal reaction in epoxy systems

Among thermosetting polymers, epoxy resins are major components of adhesives, sealants, paints and composites. Polymerization is often achieved by reaction of epoxy monomers like DGEBA with amine hardeners such as DETA. Previous works showed that polyamines interact with metal (hydr/oxide) substrates leading to the formation of an interphase involving the chelation of surface metal ions. In this work, we further explored the interaction between DETA and aluminum (hydr)oxide, denoted Al, via a combined experimental and modeling approach. We inspected in depth by DSC the modifications of glass transition temperature Tg and change in heat capacity ΔCp after curing, allowing us quantifying the impact of Al amount on DGEBA/DETA degree of cure α. A new parameter, the “percentage of inhibition of cure” denoted α¯, was defined reaching up to ~4 % in our experimental conditions. In parallel, in situ mixing calorimetry confirmed the exothermic character of DETA interaction with Al with various degrees of division. DFT calculations were carried out to examine DETA/Al3+ chelates. Among plausible chelate configurations, one was associated with a lower conformational energy and shorted Al-N bond lengths, suggesting greater stability. Calculated and experimental Raman spectra were additionally investigated, allowing us to discuss further about the DETA/Al3+ chelates at play.

Iron Complexes of Flavonoids-Antioxidant Capacity and Beyond.

Flavonoids are common plant natural products able to suppress ROS-related damage and alleviate oxidative stress. One of key mechanisms, involved in this phenomenon is chelation of transition metal ions. From a physiological perspective, iron is the most significant transition metal, because of its abundance in living organisms and ubiquitous involvement in redox processes. The chemical, pharmaceutical, and biological properties of flavonoids can be significantly affected by their interaction with transition metal ions, mainly iron. In this review, we explain the interaction of various flavonoid structures with Fe(II) and Fe(III) ions and critically discuss the influence of chelated ions on the flavonoid biochemical properties. In addition, specific biological effects of their iron metallocomplexes, such as the inhibition of iron-containing enzymes, have been included in this review.

N-Doped fluorescent carbon nanodots derived out of Gum ghatti for the fluorescence tracking of mercury ions Hg2+ in the aqueous phase

An ecofriendly microwave pyrolysis tactic was employed to synthesize N-doped fluorescent carbon nanodots (N-CNDs) using Gum ghatti and ethylenediamine as a carbon and nitrogen source. The fabricated N-CNDs exhibits high stability in water medium and emits bright blue fluorescence with the quantum yield of 23%. We further established N-CNDs as an excellent visual and selective fluorescent-based nanoprobe for mercury ions (Hg2+ ions) detection. The limit of detection was found to be 4.6 nM. When metal ion chelator ethylenediaminetetraacetic acid (EDTA) was added into the N-CNDs + Hg2+ ions system, the fluorescence was reverted effectively. Moreover, the fluorescent quenching principle of N-CNDs + Hg2+ ions system follows the static quenching mechanism. Finally, the Gum ghatti derived N-CNDs was efficiently applied in environmental water samples like tap and mineral water to determine mercury ions.