Metabolomics profiles of type 2 diabetes and insulin resistance and their associations with total mortality.

Untargeted metabolomic and lipidomic profiling in a hyperuricemic rabbit model: a pilot study.

Integrated transcriptomic and metabolomic analyses reveal the mechanism of hypoxia tolerance in Aspergillus chevalieri under controlled nitrogen atmosphere.

Metabolomic profiling and anti-biofilm activity of Majorana hortensis Moench., as well as its tissue cultures studies and molecular docking

Lipidomic, metabolomic, and proteomic profiles provide insight into differences in meat quality between rabbit longissimus lumborum and hind-leg muscles.

Diquat-induced central nervous system injury: serum multi-omics and zebrafish evidence for histidine-methylmalonate imbalance and neuroinflammation.

Revealing improvement mechanism of yak meat quality by dietary with Codonopsis pilosula stems and leaves based on transcriptomics and metabolomics.

Evaluation of rapid evaporative ionization mass spectrometry (REIMS) relationships with biochemical markers of tenderness in beef Longissimus lumborum steaks.

Sexual dimorphism in liver fibrotic metabolic dysfunction: Effects of testosterone and estrogen on CCl4-induced liver injury in ovariectomy and orchiectomy models.

Preclinical demonstration of the efficacy and mechanisms of antitumor activity of dihydroartemisinin in cholangiocarcinoma xenograft: Induction of autophagy and reprogramming of metabolism.

Metabolomics reveals early predictors of blastocyst formation in equine ICSI-derived embryos.

Beyond 100 years of vitamin E and related molecules - Milestones and open challenges.

Hepatocellular carcinoma, the third leading cause of cancer-related deaths globally, presents a critical public health burden in China due to its high incidence and mortality. While targeted therapies and immunotherapies have improved survival in advanced HCC, drug resistance remains a major therapeutic challenge. Recent studies suggest that gefitinib, an EGFR inhibitor, overcomes lenvatinib resistance, yet its mechanistic underpinnings are incompletely understood. To investigate gefitinib's metabolic effects in HCC, we conducted untargeted metabolomic profiling using two separate platforms: gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) with both hydrophilic interaction liquid chromatography (HILIC) and reversed-phase modes. Raw data were processed by Mass Hunter, normalized with internal standards, and analyzed via SIMCA for pattern recognition. Principal component analysis (PCA) of quality control samples and experimental groups (n = 6 each) confirmed system stability and clear inter-group separation. Orthogonal projections to latent structures discriminant analysis models were validated by 200 permutation tests. Analysis identified 42 metabolites with VIP > 1, of which 25 showed significant alterations (p < 0.05) post-gefitinib treatment. KEGG/RaMP-DB enrichment revealed perturbations in four key pathways: arginine-proline metabolism, nitrogen metabolism, branched-chain amino acid biosynthesis, and taurine metabolism. These results delineate gefitinib-induced metabolic reprogramming in HCC cells, providing a foundation for targeting metabolic vulnerabilities to overcome therapy resistance.

Physiological effects of long-term saline-alkaline stress on the gills of Acanthopagrus latus: A combined analysis of transcriptomics and metabolomics.

Digestibility and metabolomic profiling of camel–chickpea yogurt: Impact on rheological and bioactive properties

Gauging the effects of a chronic exposure to polystyrene nanoplastics in the liver and intestine of Carassius auratus.

Single-cell and metabolomic profiling reveals multi-lineage hepatic perturbations and metabolic reprogramming in pregnant mice exposed to dimethomorph

The gut microbiota is a key regulator of host metabolism and skeletal muscle physiology, yet its role in muscle fiber-type transformation in pigs remains unclear. Here, we used germ-free (GF) and specific pathogen-free (SPF) Rongcheng piglets to explore how microbial absence influences muscle development. GF piglets showed significantly reduced body weight and an increased proportion of fast-twitch fibers compared with SPF controls. Transcriptome sequencing identified 1332 differentially expressed genes (DEGs), notably those involved in cGMP-PKG, Thyroid hormone, HIF-1, VEGF, and Butanoate metabolism signaling pathways, which are essential for myofiber specification. Non-targeted metabolomic profiling revealed 320 differentially expressed metabolites (DEMs), with major alterations in Bile secretion. Integrated transcriptomic and metabolomic uncovered 10 KEGG pathways co-enriched with DEGs and metabolites, including Bile secretion, Ferroptosis, Inflammatory mediator regulation of TRP channels, and HIF-1 signaling pathway. Correlation analysis revealed that strong positive correlations were observed between fast-twitch fiber genes (e.g., PRKCA, MYOZ3, NFATC1) and fatty acid-related metabolites (e.g., Dodecanoic acid, Valproic Acid, and Decanoic acid), whereas negative correlations were detected between these genes and bile acid-related metabolites (e.g., Cholic acid and 7-Ketolithocholic acid), suggesting microbial metabolites is associated with muscle fiber phenotype via metabolic and signaling crosstalk. Collectively, our findings demonstrate that gut microbial absence in pigs disrupts bile acid metabolism and fatty acid metabolism, which in turn drives the shift of skeletal muscle fiber composition toward fast-twitch glycolytic fibers. These metabolites are identified as key microbial mediators linking the gut microbiota to the transcriptional regulatory function of porcine muscle fiber type specialization, providing new insights into the gut-muscle axis and highlighting the important role of microbiota-derived metabolites in maintaining porcine muscle fiber composition and metabolic balance.

Optimization of lens size and ion source parameters for gas chromatography/mass spectrometry-based metabolomics using hydrogen as a carrier gas.

Non-additive effects of microplastics, atrazine, and sulfamethoxazole co-exposure on Penaeus vannamei health and environmental risks.