Melaleuca Cajuputi Research Articles

ABSTRACT. Essential oil (EO) was extracted from Melaleuca cajuputi Powell leaves using the steam distillation method. The main aim of this study is to determine several physicochemical properties of EO, including acid value, saponification value, ester value, relative density, absolute density, and freezing point. In addition, the chemical components were also analyzed using the gas chromatography–mass spectrometry (GC–MS) method. The obtained 1,8-cineol content is quite high (71.83%). In addition, these components in EO had an antioxidant capacity and antibacterial activity, including gram-positive and gram-negative bacteria (using the paper disc diffusion method for antibiotic susceptibility testing). Particularly, this EO inhibited the growth of Escherichia coli (ATCC 25922), Salmonella enteritidis (ATCC 13076), and Staphylococcus aureus (ATCC 25923).
 
 KEY WORDS: Antimicrobial activity, Antioxidant capacity, GC-MS, Essential oil, Melaleuca cajuputi Powell.
 
 Bull. Chem. Soc. Ethiop. 2021, 35(3), 677-683.
 DOI: https://dx.doi.org/10.4314/bcse.v35i3.18

Coal mining is one of economic sector that requires forestry area in Indonesia. Most of coal mining concession are forestry area loan use from the government and during mining operation facing with acid mine drainage (AMD). AMD passive treatment can be integrated with revegetation process that related with better handling of overburden (OB) with potential acid forming (PAF) during reclamation processed. The most expensive methods and uncertainty process are the use of quick lime in treatment of AMD. During operation and preparing mine closure stage need to create the effective one to comply with the regulation. “Swampy Forest (SF) system” is an alternative method of sustainable and naturally process. Selecting the appropriate tree species as a preliminary process to develop a better SF system by batch experiment in the form of a forest constructed wetland. The tree species selected are Nauclea orientalis (lonkida tree) and Melaleuca cajuputi (kayuputih tree) as non-local species then Nauclea subdita (bangkal tree) and Melaleuca leucadendron (galam tree) as local species have been selected. Local species higher in Fe and Mn adsorption in pH of AMD <4 then nonlocal species higher in Fe and Mn adsorption on pH of AMD >4 condition.

Proapoptotic activity of essential oils from Syzygium aromaticum, Melaleuca cajuputi, and Cymbopogon nardus on HeLa human cervical cancer cells

Phytoconstituents and biological activities of Melaleuca cajuputi Powell: A scoping review

UV irradiation can cause cutaneous damage that may be specific according to the wavelength of UV rays. For example, damage from UVB irradiation manifests itself in the form of sunburn cells and enhancement of the expression of p53, while damage from UVA exposure results in an increase in the expression of vimentin. These reactions to UV irradiation were used in this work to evaluate the photoprotective capacities of two sunblock preparations that were applied to the surface of the skin. One sunblock preparation is a UVB absorber containing the coastal plant of Melaleuca cajuputi essential oil and titanium oxide (TiO2) exclusively. The other sunblock preparation is a conventional sunblock containing oxybenzone as comparison. Result obtained for UV-Vis test was observed that the new formulation derived showed good absorption and exhibit high potential to be further develop as sunscreen in cosmeceutical applications

Kebakaran sering terjadi di hutan hujan tropis terutama di musim kemarau. Salah satu dampaknya yaitu berkurangnya biomassa dan menurunkan kandungan karbon. Tujuan penelitian adalah melakukan evaluasi sediaan karbon pada hutan bekas terbakar berulang. Penelitian dilakukan pada bulan Oktober 2019 di hutan sekunder terbakar pada tiga lokasi terpilih yaitu lokasi kebakaran tahun 2015, lokasi kebakaran tahun 2017, serta lokasi kebakaran berulang tahun 1997, 2015 dan 2017 yang berada di areal kerja PT Bumi Andalas Permai, Provinsi Sumatera Selatan. Pada masing-masing lokasi, plot penelitian dibuat seluas satu Ha (100 x 100 m). Penelitian menunjukkan bahwa komposisi tegakan tingkat pohon, tiang dan semai di hutan terbakar tahun 2015 didominasi jenis uba (Syzygium sp.), pada hutan terbakar tahun 2017 didominasi jenis Acacia crassicarpa dan Macaranga sp., sedangkan pada hutan yang terbakar berulang tahun 1997, 2015 dan 2017 didominasi oleh jenis gelam (Melaleuca cajuputi). Sediaan karbon pada tegakan berdiameter ≥10 cm secara umum sangat rendah, yaitu sebesar 20,06 ton C/Ha pada tegakan hutan terbakar tahun 2015, sebesar 0,42 ton C/Ha di hutan terbakar tahun 2017 dan sebesar 0,16 ton C/Ha pada hutan terbakar berulang tahun 1997, 2015 dan 2017. Kebakaran hutan berulang berdampak pada turunnya biomassa dan jumlah pohon per-ha, sehingga untuk memulihkannya diperlukan restorasi dan kerja yang ektra keras mencegah kebakaran.

Abstract. Faradilla FA, Prihatini I, Suranto, Susilowati A. 2022. Genetic variation of Austropuccinia psidii in some species of Myrtaceae as host plants in Java, Indonesia based on simple sequence repeats (SSR) markers. Biodiversitas 23: 256-263. Austropuccinia psidii is pathogenic rust with a wide host and is considered a biosecurity threat to the Myrtaceae family in many countries. The genetic variation of this rust in Java, Indonesia is poorly reported. Therefore, this study aimed to determine the genetic variation among A. psidii from different Myrtaceae hosts, namely Syzygium polyanthum, S. myrtifolium, and Melaleuca cajuputi from three different locations in Java using seven simple sequence repeats (SSR) markers. Data were collected from 28 A. psidiisamples from three different hosts and locations in Java. The genetic variations of A. psidii were found in six isolates in locus USYD_Pp168, PpSSR161, and PpSSR195*. The results showed that the expected heterozygosity value among 12 isolates of A. psidii is 0.312. The dendrogram illustrates two clusters constructed with cluster I consisting of subcluster IA (S1, S3, S4, S5, K1, K2, K3, K4, and P1) and IB (P2); and cluster II consists of isolates S2 and S6. Principal Coordinate Analysis (PCoA) was used to demonstrate the similarity and dissimilarity among isolates based on microsatellite sites. Axis 1 and 2 explained 87.7 % of the total variations and sep a rated isolate S1, S2, S3, S4, S6 from other isolates, and grouping the rest of isolates together. The dendrogram and PCo analysis demonstrated A. psidii isolates tend to have close genetic similarity based on their host.

Non-Timber Forest Products (NTFPs) management can lead to various benefits for community livelihood and forest sustainability. However, such management has not been carried out optimally and sustainably in Indonesia, due to various limiting factors including ineffective policies, undeveloped cultivation technologies, and inadequate innovation in processing technologies. Further, the diversity of NTFPs species requires that policy-makers determine the priority species to be developed. Agarwood (Aquilaria spp. and Gyrinops spp.), benzoin (Styrax spp.), sandalwood (Santalum album L.), and cajuput (Melaleuca cajuputi Powell) are aromatic NTFPs species in Indonesia that forest-dwellers have utilized across generations. This paper reviews the current governance, cultivation systems, processing and valuation, and benefits and uses of these species. We also highlights the future challenges and prospects of these NTFPs species, which are expected to be useful in designing NTFPs governance, in order to maximize the associated benefits for the farmers and all related stakeholders.

Melaleuca cajuputi breeding in Indonesia is entering the advanced generation cycle and improvements have been achieved for oil concentration and 1.8 cineole-content. In commercial plantations, the total oil yield is an important factor to ensure the sustainability and continuity of oil production. This variable is calculated based on oil concentration, survival rate, and leaf biomass. However, to date, biomass productivity is maintained through silviculture practices rather than genetics. Therefore, genetic improvement for other traits related to leaf biomass is necessary. This study aimed to optimize the breeding strategy of M. cajuputi for a multiple-trait selection using the economic weight of traits related to oil yield. The economic weight was derived by combining selection results in the past generation breeding population and the assessment in genetic gain trials. The study revealed that leaf biomass should be prioritized as selection criteria for oil concentration in the advanced generation breeding based on the current baseline of the achieved gain. The implication of the economic weight to further generation breeding selection for improving oil yield productivity is that the major traits affecting the oil yield should be incorporated simultaneously for selection in the breeding strategy of M. cajuputi. The leaves biomass could be more weighted than other traits in constructing the index for the multiple-trait selection considering the correlation among the three traits observed.

Gelam tree or Melaleuca cajuputi (M. cajuputi) is an important species for the local economy as well as coastal ecosystem protection in Terengganu, Malaysia. This study aimed at producing a current habitat suitability map and predicting future potential habitat distribution for M. cajuputi in Terengganu based on Species distribution modeling (SDM) using the Maximum Entropy principle. Our modeling results show that for the current potential distribution of M. cajuputi species, only 10.82% (1346.5 km2) of Terengganu area is suitable habitat, which 0.96% of the areas are under high, 2.44% moderate and 7.42% poor habitat suitability. The model prediction for future projection shows that the habitat suitability for M. cajuputi would decrease significantly in the year 2050 under RCP 4.5 where the largest contraction from suitable to unsuitable habitat area is about 442.1 km2 and under RCP 2.6 is the highest expansion from unsuitable to suitable habitat area (267.5 km2). From the future habitat suitability projection, we found that the habitat suitability in Marang would degrade significantly under all climate scenarios, while in Setiu the habitat suitability for M. cajuputi remains stable throughout the climate change scenarios. The modeling prediction shows a significant influence on the soil properties, temperature, and precipitation during monsoon months. These results could benefit conservationist and policymakers for decision making. The present model could also give a perception into potential habitat suitability of M. cajuputi in the future and to improve our understanding of the species’ response under the changing climate.

Abstract. Alam T, Suryanto P, Supriyanta, Basunanda P, Wulandari RA, Kastono D, Widyawan MH, Nurmansyah, Taryono. 2021. Rice cultivar selection in an agroforestry system through GGE-biplot and EBLUP. Biodiversitas 22: 4750-4757. Genotype-by-environment interaction (GEI) causes differences in the productivity of rice cultivars in agroforestry systems. For this reason, the stability of rice cultivars is an important aspect that should be considered before a cultivar is recommended to farmers. Superior genotypes and ideal environments are commonly identified using two statistical models, namely, genotype–genotype-by-environment biplot (GGE-biplot) and empirical best linear unbiased prediction (EBLUP). In this study, 15 rice cultivars were evaluated in terms of their productivity and stability in three soil types (Lithic Haplusterts, Ustic Epiaquerts, and Vertic Haplustalfs) in an agroforestry system with kayu putih (Melaleuca cajuputi) in 2019 and 2020 at the Menggoran Forest Resort, Playen Forest Section, Yogyakarta Forest Management District, Indonesia. The cultivars were treated as random effects to select and obtain the EBLUP of the best cultivars in each soil type. The EBLUP revealed that Situ Patenggang showed the highest yields of 4.887 and 5.456 tons ha?1 in Lithic Haplusterts and Vertic Haplustalfs, respectively. GM 28 exhibited the highest yield of 6.492 tons ha?1 in Ustic Epiaquerts. Ciherang, GM 2, GM 8, GM 11, GM 28, Inpari 6 Jete, Inpari 33, IR-64, and Way Apo Buru were classified as stable and fairly stable cultivars, whereas the other cultivars were unstable. Therefore, rice cultivars with high yields in specific soil types should be selected.

The kinetics of antibacterial activity of cajuput oil (Melaleuca cajuputi) to E. coli, B. cereus, and S. aureus were investigated. The aim of this study was to determine the reaction orders of cajuput oil as an antibacterial agent. The extraction of cajuput oil was conducted by water-steam distillation. The yield was 0.88%. GC-MS analysis showed that the cajuput oil contained β-ocimene (19.35%), 1.8-cineole (17.67%), limonene (12.09%), β-caryophyllene (9.51%), γ-terpinene (8.93%), 2-β-pinene (8.85%), α-terpinolene (4.96%), α-humulene (4.10%), α-terpineol (2.83%), and p-cymene (2.33%). The extract showed antibacterial activity to E. coli, B. cereus, and S. aureus, with the reaction orders of 0.4460, 0.8235 and 0.6928, respectively.

Tumih (Combretocarpus rotundatus (Miq.) Danser) and Galam (Melaleuca cajuputi) are typical vegetation of the wetlands of South Kalimantan. Tumih and galam wood waste can be made into charcoal briquettes which have economic value. The aims of this study were: 1) Analyzing the characteristics of charcoal briquettes, namely: water content, density, ash content, volatile matter content, bound carbon content and calorific value and 2) Knowing the best treatment from a variety of treatments. The design model used was a completely randomized design (CRD) with 4 treatments and 3 replications. The process of making charcoal briquettes at the Forest Products Technology Laboratory. Testing the characteristics of charcoal briquettes was done at the Laboratory of the Research and Industrial Standardization Institute (BARISTAND) Banjarbaru. The results of the analysis of diversity showed that the mixed treatment of tumih wood waste and galam wood waste had a very significant effect on water content, ash content, volatile matter content, bound carbon content and calorific value, but had no significant effect on density. The water content of charcoal briquettes from galam wood waste and tumih wood waste and the combination of a mixture of galam wood waste and tumih wood waste ranged from 6.1133-10.6667 %, the average density value was between 0.5228-0.5897 g/cm3, the average value The average ash content is between 1.3000-2.9300%, the volatile matter content shows an average range of 41.6333-48.4767%, the average value of bound carbon content is 37.9267-50.5400% and the average calorific value ranged from 5084.41 to 6230.59 cal/g. Treatment A3 (25% galam wood waste + 75% tumih wood waste is the best treatment and meets American standards for moisture content and calorific value. Ash content of all treatments are A1. A2, A3 and A4 meet American standards.

<p class="02abstracttext">In recent few years, many works have been dedicated to search for new source of renewable energy. In this study, new source of renewable energy is based in the briquette made from mixture of ironwood (Eusideroxylon zwageri) powder and gelam (Melaleuca leucadendron) wood powder. The mixture was carbonized at temperature of 500 <sup>o</sup>C ± 10 <sup>o</sup>C for 120 minutes. The size of the particles used was 50 mesh while the ratio between wood powder adhesives, i.e. starch powder, used in the study was 1:1. The composition variations between ironwood charcoal and gelam were 100% ironwood charcoal powder and 0% gelam, 70%: 30%, 50%: 50%, 30%: 70%, 0% ironwood: 100% gelam. The compaction pressure during briquette production was varied at 100 kg/cm<sup>2</sup>, 125 kg/cm<sup>2</sup> and 150 kg/cm<sup>2</sup>. The highest combustion temperature at 205 <sup>o</sup>C occurred in the specimen with composition of 30% ironwood powder and 70% gelam wood powder with compaction pressure of 100 kg/cm<sup>2</sup>. The longest burning duration 140 minutes (2 hours 20 minutes) occurred at composition of 50% ironwood powder and 50% gelam wood powder at compaction pressure of 150 kg/cm<sup>2</sup>. The fastest initial ignition time was 7 minutes and occurred for mixture with composition of 70% ironwood powder and 30% gelam wood powder at compaction pressure of 125 kg/cm<sup>2</sup>.</p>

Melaleuca cajuput (cajuput) subsp. cajuputi is an endemic species of Indonesia, found naturally in the island of Buru, Seram and Ambon and cultivated in Java. The cajuputi tree is harvested for the leaves which contain important essential oil of 1,8 cineole. Because of intensive utilization and inappropriate management of the resources, the genetic diversity in the natural distribution is under threat. Collection of genetic materials across its natural distribution conducted in 1995 and 1998 had captured more than 160 genotypes for screening of high oil yield and 1,8 cineole content. The selected genotypes had been planted as seed orchards in Java for production of genetically improved seeds. Breeding program is continuing to produce elite clones. The use of superior seeds/clones for plantation is the best solution to increase cajuput oil production and to achieve self-sufficiency in oil supply. Both industrial scale and smallholder plantations are being established to increase the national production which currently stood at around 650 ton/year whilst domestic demand is already over 3500 ton/year. The species is a good example of successful domestication. The native stands are still utilized for oil production and plantations outside the natural range are developing. The paper also discusses importance of breeding program to increase cajuputi oil production in Indonesia.

Waste of cajuput leaves (Melaleuca cajuputi Powell) has the potential to be used as an alternative cattle feed because it has a fairly high nutritional content. The nutritional content of cajuput leaf waste needs to be improved before being used as an alternative feed. The fermentation process can increase nutrient content in cajuput leaf waste and increase the digestibility of organic matter (OMD), dry matter digestibility (DMD), Ammonia (NH3), and Volatile Fatty Acid (VFA). This study aimed to determine the improvement of cajuput leaf waste nutrition with the help of yeast as well as the effect of fermented cajuput leaf waste along with a mixture of concentrates and field grass on digestibility, NH3, and VFA which were carried out in vitro test. The results showed the nutritional content of cajuput leaf waste fermented by yeast of Aspergillus chevalieri had the best value of protein 16.03%, fiber 16.92%, and fat 5.93%. The treatment R4 (50% cajuput leaf waste + 25% concentrate + 25% field grass) had the best digestibility (DMD=46.12%; DMO=32.08%), NH3 (8.37mM) and VFA (168.5mM) production.

Galam (Melaleuca cajuputi subsp. Cumingiana (Turcz.) Barlow) is a peat swamp forest plant. This plant is used to treat respiratory diseases, colitis, sore throat, itchy skin, diarrhea, and dizziness. This study aimed to analyze the antibacterial and antifungal activity of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit against Escherichia coli and Candida albicans bacteria. This research is a pretest-posttest research with control group design and consists of 3 stages of research. The first stage is the phytochemical screening test, the second stage determines the minimum inhibitory concentration (MIC) and the third determines the minimum bactericidal concentration (MBC). The results showed that the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit contained terpenoids, flavonoids, phenols, saponins, quinones, steroids, tannins, while alkaloid compounds only found in the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit. The MIC and MBC values ​​of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower methanol extract against E. coli were 1.67 and 2.083 mg/mL, respectively, while the MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower against C. albicans were 3.125 and 25 mg/mL. The MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against E. coli have same value were 3.334 mg/mL, while the MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against C. albicans were 3.125 and 12.5 mg/mL, respectively.
 Keywords: Galam antibacterial, antifungal, Escherichia coli, Candida albicans

Modelling the susceptibility of wetland plant species under climate change in the Mekong Delta, Vietnam

Some types of fungi are known to have the ability to produce Indole Acetic Acid (IAA). Fungi can be isolated from the rhizosphere and tissues of various plants, including from the rhizosphere and the root "Galam" (Melaleuca cajuputi Powell.), which grow predominantly in peatlands. Therefore, the purposes of this study were: (a) to isolate and measure the potential of fungi from endophytic and rhizospheric of “Galam”(M. cajuputi) as a producer of IAA hormone, (b) determine the types of fungal interaction that occur and their potential to increase the total IAA hormone produced. This research begins with isolation, purification, isolate screening, analysis of IAA hormone production, data analysis, seed germination test and isolates identification. The result showed that the concentration of IAA produced by Penicillium sp. IRZ15 was 5.86 ± 0.47 μg.mL-1 to 8.46 ± 0.26 μg.mL-1 and Syncephalastrum sp. AG15 is 4.77 ± 0.44 μg.mL-1 to 8.77 ± 0.25 μg.mL-1. Meanwhile, the combination of rhizospheric fungi Penicillium sp. IRZ15 and endophytic fungi Syncephalastrum sp. AG15 does not produce significantly different IAA concentrations (6.42 ± 0.34 μg.mL-1 to 9.19 ± 0.50 μg.mL-1 ) compared to fungi used alone without combinations.

This study was conducted to assess the water quality affected by different land use patterns in U Minh Ha National Park, Ca Mau, Vietnam. This study determined the water quality characteristics in three land use types (Acacia hybrid, planted melaleuca cajuputi, and natural melaleuca cajuputi) at different plant ages on two acid sulfate soil layers in the rainy season (8/2018) and dry season (4/2019) using nine water quality parameters. Multivariate statistical analyses were applied to evaluate the correlation and spatial and temporal variations in the water quality. The study results showed that the water quality in S-ASS was more polluted than that in D-ASS, characterized by low pH; the EC, organic matters (BOD and COD), nutrients (N-NH4+ and N-NO3−), and metal ions (Al3+ and Fe3+) were high; and the EC, BOD, COD, Al3+, and N-NO3− were determined high in D-ASS. The NMC area was noted to have high concentrations of organic matters and nutrients, while the factors specific to acidic soil were found to be higher in the AH and PMC areas. The water quality in the rainy season tended to be more polluted than that in the dry season. The cluster analysis grouped the land use patterns on S-ASS and D-ASS in both seasons into four groups, with a clear similarity between the wet and dry seasons in the areas at various plant ages. The seasonal variations of the water quality of the three land use types were distinguished by the main parameters, including pH, EC, BOD, N-NO3−, and Al3+ (S-ASS) and EC, BOD, N-NO3−, N-NH4+, and Fe3+ (D-ASS). Therefore, there is a need for better water management measures in the rainy season and focus on the key parameters causing water quality variations in each area. The findings in this study provided important information for the future water quality monitoring for both agricultural production and conservation in the national park.