Medullary Collecting Duct Function Research Articles

Effects of increased perfusion pressure on medullary collecting duct function

The role of the medullary collecting duct in pressure natriuresis has not been established. In vivo microcatheterization was used to study the effect of an acute increase in blood pressure induced by bilateral carotid artery and vagal nerve ligation on medullary collecting duct function in anaesthetized rats. Increased fluid and electrolyte excretion during pressure natriuresis were accompanied by increased delivery of water, sodium, chloride, and potassium to the beginning of the medullary collecting duct, a change that was significantly greater than in a second series of time-control animals. These increases in delivery were within the range for which constant fractional NaCl reabsorption had been found previously. However, during increased perfusion pressure, reabsorption of both sodium and chloride in the medullary collecting duct as a fraction of delivered load were reduced from 81 +/- 4.1 to 51 +/- 9.3% (p less than 0.01) and from 65.7 +/- 6.0 to 42.7 +/- 9.1% (p less than 0.01), respectively. No significant changes in medullary collecting reabsorption were seen in the time controls. We conclude that increased perfusion pressure, in addition to increasing delivery to the medullary collecting duct, also inhibits sodium chloride reabsorption in this nephron segment.

Collecting duct function in cis-platinum nephrotoxicity.

Microcatheterization was used to study the effect of cis-platinum nephrotoxicity on inner medullary collecting duct function in anaesthetized rats. Osmolality of collecting duct fluid increased from the beginning to the end (papillary tip) of the collecting duct by only 69 +/- 11 mosmol/kg in cis-platinum treated rats (at 5-6 days) compared with 306 +/- 75 mosmol/kg in sham controls (p less than 0.01). Tubular fluid to plasma inulin concentration ratio was reduced at the beginning and end of the collecting duct. Tubular fluid sodium, chloride, and potassium concentrations were lower at the papillary tip in cis-platinum treated rats (p less than 0.01). The results indicate that collecting duct water reabsorption is reduced, but electrolyte reabsorption is normal (or even increased) in cis-platinum nephrotoxicity. Papillary tissue sodium chloride concentration was reduced in cis-platinum treated rats. We conclude that the characteristic decrease in urine concentrating ability in cis-platinum nephrotoxicity is not primarily the result of an intrinsic abnormality in collecting duct function but is secondary to decreased papillary hypertonicity resulting from impaired function in more proximal nephron segments, presumably the pars recta of the proximal tubule and the loop of Henle where previous studies have demonstrated abnormal function.

Inner medullary collecting duct function during rebound alkalemia.

Rats, made acidemic when fed NH4Cl, become alkalemic with discontinuation of the NH4Cl. This phenomenon has been called rebound metabolic alkalemia (RMA). This study examines the function of the inner medullary collecting duct (IMCD) during RMA. Rats drank only 1.5% NH4Cl for 5 days and then water for 16 h prior to study, yielding an arterial pH = 7.50 +/- 0.01, PCO2 = 39 +/- 1 mmHg, and bicarbonate = 29.5 +/- 1.0 mM. The IMCD data were obtained by microcatheterization from deep (1.5-3.0 mm) and tip (0.2-0.5 mm) samples. Equilibrium pH decreased from 5.92 +/- 0.09 (n = 20) to 5.38 +/- 0.04 (n = 20) and PCO2 increased from 32 +/- 1 to 38 +/- 1 mmHg between deep and tip samples. Bicarbonate delivery decreased from 37 +/- 8 to 7 +/- 1 nmol/min. Titratable acid and ammonium delivery increased from 284 +/- 52 to 347 +/- 62 nmol/min and from 549 +/- 38 to 685 +/- 40 nmol/min, respectively. Calculated net acid excretion increased from 796 +/- 88 to 1,026 +/- 95 nmol/min. Thus during RMA, proton secretion continues along the IMCD, although there is a systemic alkalemia. It appears that factors in addition to systemic acid-base parameters are important in the regulation of proton secretion by the IMCD.

Effect of acetylcholine and secretin on medullary collecting duct function in the rat.

Microcatheterization was used to study the effect of renal arterial infusion of acetylcholine or secretin on medullary collecting duct function in anaesthetized rats. Acetylcholine infusion was associated with natriuresis and increased sodium delivery to, and decreased reabsorption in, the collecting duct. No changes from control function were found with secretin. Renal blood flow was increased with acetylcholine (+82%, p less than 0.001), but unchanged with secretin (+15%, nonsignificant). We conclude that acetylcholine natriuresis is due to inhibition of tubular reabsorption of sodium in the medullary collecting duct, as well as in upstream nephron segments. While the latter may be hemodynamically mediated, the former indicates a direct transport effect of the hormone in the terminal nephron segment.

Thiazide diuretic effect on medullary collecting duct function in the rat

The distal convoluted tubule is thought to be the principal site of action of thiazide diuretics, but, to our knowledge, there are no studies of their possible effects on collecting duct transport. Microcatheterization of the inner medullary collecting duct (IMCD) was carried out in rats undergoing a modest diuresis, natriuresis, and chloriuresis from hydro-chlorothiazide (2 mg/kg/hr) and in normal controls. Delivery of fluid, sodium, and chloride to the beginning of the IMCD was increased, but not significantly, while the load remaining at the papillary tip (end) of the duct was increased markedly by hydrochlorothiazide. Chloride reabsorption in the IMCD was affected most markedly; the chloride reabsorption between the beginning and end of the duct, as a fraction of the delivered load, was reduced from 70.4 +/- 5.4% in controls to insignificant amounts with hydrochlorothiazide (8.2 +/- 11.5%, P less than 0.001). The fraction of delivered sodium reabsorbed along the collecting duct was decreased from 78.5 +/- 4.9% in controls to 37.2 +/- 12.4% (P less than 0.005) in thiazide-treated rats and fluid reabsorption was decreased from 59.4 +/- 4.0% in controls to 31.9 +/- 5.1% (P less than 0.005). Small but significant potassium secretion into the IMCD occurred with hydrochlorothiazide, probably secondary to the marked increase in potassium delivery to the duct. Increased potassium excretion could account for a maximum of 50% of chloriuresis with hydrochlorothiazide. The observation that thiazide diuretics decrease chloride, sodium, and fluid reabsorption in the medullary collecting duct, like the recently demonstrated inhibitory effect of furosemide on this nephron segment, has significant implications for the rationale for diuretic use.

Effect of adrenal enucleation on inner medullary collecting duct function in the rat

Page F453: H. H. Bengele and E. A. Alexander. “Effect of adrenal enucleation on inner medullary collecting duct function in the rat.” Page F456: The following acknowledgment should be added: The accomplishment of this work was helped by the National Biotechnology Resource in Electron Probe Microanalysis, Claude Lechene Director, Harvard Medical School. This work was supported by Grant 5-P41-RR00679 from the National Institutes of Health.

Urea reabsorption in the medullary collecting duct of protein-depleted young rats before and after urea infusion.

The aims of the present study were to examine urea handling along the length of the medullary collecting duct (MCD) in protein-depleted young rats and to determine the effect of urea infusion on MCD function and urine concentrating ability. In 10 young rats on a low protein diet, urea reabsorption equivalent to 18.3% of the filtered load was observed along the MCD (4.5 mm) using the microcatheterization technique. Collecting duct urea reabsorption occurred almost entirely (16.6%) in the distal portion of the MCD (mid-zone to papillary tip, 2.8 mm). These results are in contrast to the lack of net urea reabsorption along the MCD in protein-replete adult rats [21]. After urea infusion which raised plasma urea level from 3.5 to 10.5 mmol/l in protein-depleted rats, urine non-urea solute concentration increased in the non-exposed right kidney from 827 to 1,199 mosm kg−1 (P<0.001) but the increase was not significant in the experimental left kidney (590 to 619 mosm kg−1). Thus exposure of the papilla interfered with urea-induced enhancement of urine concentrating ability. After urea infusion, fractional urea reabsorption in the distal portion of the MCD was similar to that before infusion (21.1%) of filtered load) but the absolute load of urea delivered to the MCD and reabsorbed along the duct was markedly increased (2.7-fold). In 6 rats with an increase in urine non-urea solute concentration in the experimental kidney after urea infusion, fluid reabsorption along the duct was significantly increased. The results indicate that in protein-depleted young rats (1) there is significant urea reabsorption in the distal portion of the medullary collecting duct, (2) urea infusion contributes to enhanced urine concentrating ability, in part, by increasing absolute urea reabsorption and also water reabsorption in the collecting duct.

Medullary collecting duct function in the remnant kidney before and after volume expansion

The role of the medullary collecting ducts (CD) in the regulation of water and electrolyte excretion by the remnant kidney has not been determined. Medullary CD function was therefore studied by the microcatheterization technique during hydropenia and after volume expansion with an isotonic saline load in rats with sham-operated normal kidney (stage I), remnant kidney (stage II), remnant kidney after contralateral nephrectomy (stage III), and sham-operated normal kidney after contralateral nephrectomy (stage-III control). Sodium, potassium, water, and solute reabsorption along the medullary CD were not altered in stage II during hydropenia when compared to normal control (stage I), but water reabsorption proximal to the medullary CD was decreased. In stage III, where the uremia was mild (BUN, 41 mg/dl), the fractions of the filtered load of sodium (72%) and water (62%) which were reabsorbed along the medullary CD were reduced in comparison to stage II (94% and 83%, respectively), stage I, or stage-III control. The fraction of filtered potassium entering the medullary CD was increased to 53% in stage III, compared to 16% in stage II, and 10 or 11% in stage I or stage-III control. No change in the fraction of filtered potassium remaining along the collecting ducts was observed. After volume expansion, there was no significant change in the fraction of filtered sodium and water remaining along the medullary CD in stage I, II, or III. The greater fractional excretion in stage III resulted from decreased reabsorption in more proximal nephron segments. The results indicate that (a) during hydropenia, fractional reabsorption of sodium and water is decreased along the medullary CD of the stage-III remnant kidney in the presence of mild uremia, (b) the increased fractional excretion of potassium in the remnant kidney with uremia is not determined by altered potassium handling in the medullary CD but occurs proximal to this nephron segment, and (c) extracellular fluid volume expansion with isotonic saline results in similar inhibition of fractional sodium and water reabsorption in the collecting ducts of both remnant and normal kidneys.

Effects of furosemide, acetazolamide, and mannitol on medullary collecting-duct function in the rat kidney.

The microcatheterization technique was used to study transport of fluid, sodium, and potassium in the medullary collecting duct in rats (232–357 g) before and during administration of diuretic drugs. Series I received furosemide (1.5 mg initial dose + 1.5 mg/h), Series II acetazolamide (0.75 mg +0.75 mg/h), and Series III and IV mannitol at 0.3 g+0.3 g/h, and 0.3 g+1.0 g/h, respectively. All diuretics caused diuresis, natriuresis, and kaliuresis. The renal response to furosemide and acetazolamide was associated with increased hematocrit and decreased filtration rate, indicating depletion of blood volume. No such effect was seen in Series III and IV. In the collecting duct furosemide completely abolished the normal reabsorption of sodium and fluid and initiated net secretion of potassium. Partial inhibition of salt and water transport in the collecting duct was observed with acetazolamide and the low dose of mannitol (III). Both treatments resulted in potassium secretion. In Series IV the high rate of mannitol infusion was associated with complete inhibition of salt and water reabsorption from the medullary collecting system similar to that of Series I. The greater excretory response in this group compared to the furosemide series was due to increased delivery of tubular load to the collecting duct. It is concluded that a major site of action of furosemide is in the medullary duct, resulting in quantitative inhibition of salt and water reabsorption from this nephron segment. The partial transport inhibition during acetazolamide or modest mannitol diuresis can be explained by the presence of poorly absorbable solute in duct fluid. The mechanism of inhibition of reabsorption after the high rate of mannitol infusion remains undetermined.

Effect of adrenalectomy on medullary collecting-duct function in rats before and during blood volume expansion.

Adrenalectomized rats, kept on tap water for 3 days and infused with the glucocorticoid dexamethasone during the experiment, were compared to similarly treated sham-operated rats. Using the microcatheterization technique, reabsorption of fluid, sodium and potassium in the medullary collecting duct was studied before and after infusion of donor blood (2.3% of body weight). Before intravascular volume expansion sodium excretion in adrenalectomized rats was greater than in sham-operated ones. Extensive overlap between the two groups made this difference not statistically significant. However, the fraction of filtered sodium excreted was significantly greater after adrenalectomy, indicating the expected tubular transport defect. Fluid reabsorption from the medullary collecting-duct system was comparable in both series. Adrenalectomy did not inhibit net sodium reabsorption from the inner medullary duct, although reduction of Na transport in the outer medullary collecting system could be inferred. Renal excretion of potassium was not associated with net K secretion in the collecting duct in either group. During hypervolemia induced by intravenous infusion of donor blood, marked diuresis, natriuresis and kaliuresis were observed in all animals, associated with inhibition of net fluid and sodium reabsorption along the collecting system in both inner and outer medulla. Small, but statistically significant secretion of potassium became evident. The relatively reduced renal response in adrenalectomized animals could be attributed in part to a decreased filtered load compared to sham-operated rats. It is concluded: (1) that lack of mineralocorticoid does not prevent the normal fluid and sodium reabsorption from the lumen of the inner medullary collecting system, and (2) that the inhibition of this reabsorption consequent to hypervolemia is independent of changes in plasma aldosterone levels.

The role of the medullary collecting ducts in postobstructive diuresis.

Medullary collecting duct function was studied by direct microcatheterization techniques in rats undergoing postobstructive diuresis. Significant net addition of water and sodium to the duct was demonstrated during postobstructive diuresis after relief of 24-h bilateral ureteral ligation. This striking abnormality in function was associated with reduced delivery of sodium and water to the collecting duct compared to sham-operated controls. To examine the role of circulating factors in this phenomenon, another group of rats was studied that underwent 24 h of total urine reinfusion into the femoral vein. Natriuresis and diuresis were similar to the postobstructive group, but absolute collecting duct reabsorption of sodium and water was normal. The natriuresis and diuresis in rats with urine reinfusion resulted from increased delivery of fluid and sodium to the medullary collecting duct. A third group of rats was studied with 24-h unilateral ureteral ligation as well as urine reinfusion from the contralateral normal kidney. Without urine reinfusion there was no diuresis-natriuresis but with urine reinfusion the diuresis and natriuresis after relief of unilateral obstruction was similar to that after relief of bilateral obstruction. Moreover, net addition of sodium and no significant water reabsorption were demonstrated in the medullary collecting duct of such animals. The results indicate that (a) the medullary collecting duct is the critical nephron segment affected by ureteral obstruction, since postobstructive diuresis occurred despite reduced delivery of fluid from the more proximal nephron; (b) the net addition of sodium to the medullary collecting duct observed during postobstructive diuresis is probably a direct effect of obstruction, since it was found during postobstructive diuresis after relief of bilateral or unilateral ureteral ligation, but not with urine reinfusion alone; and (c) blood-borne factors are important in the development of postobstructive natriuresis and diuresis, and probably act by increasing the fraction of filtered sodium and water delivered from the proximal and distal tubule to the collecting duct.

Evidence for urinary dilution by the collecting tubule.

ARTICLESEvidence for urinary dilution by the collecting tubuleRL Jamison, and FB LacyRL Jamison, and FB LacyPublished Online:01 Oct 1972https://doi.org/10.1152/ajplegacy.1972.223.4.898MoreSectionsPDF (1 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByUrine Concentration and DilutionThe renal segmental distribution of claudins changes with developmentKidney International, Vol. 62, No. 2Renal Actions of Vasopressin1 January 2011Requirement of aquaporin-1 for NaCl-driven water transport across descending vasa rectaJournal of Clinical Investigation, Vol. 105, No. 2Urinary Concentration and Dilution: Physiology1 January 2011Ultrastructure of the nephron of the young camelCamelus dromedariusJournal of Morphology, Vol. 210, No. 2Mechanisms and regulation of ion transport in the renal collecting ductComparative Biochemistry and Physiology Part A: Physiology, Vol. 90, No. 4Relative Density of Urine: Methods and Clinical Significance27 September 2008 | CRC Critical Reviews in Clinical Laboratory Sciences, Vol. 26, No. 3Mechanism of Concentration and Dilution of UrineStimulation by human calcitonin of electrolyte transport in distal tubules of rat kidneyPflügers Archiv, Vol. 399, No. 2A patient with polyuria and hyponatremiaKidney International, Vol. 24, No. 2Thiazide diuretic effect on medullary collecting duct function in the ratKidney International, Vol. 23, No. 5BIBLIOGRAPHYAnnals of the New York Academy of Sciences, Vol. 394, No. 1 The BrattleboDisorders of urinary concentration and dilutionThe American Journal of Medicine, Vol. 72, No. 2Functional characterization of drug-induced experimental papillary necrosisKidney International, Vol. 15, No. 3Effects of furosemide, acetazolamide, and mannitol on medullary collecting-duct function in the rat kidneyPfl�gers Archiv European Journal of Physiology, Vol. 373, No. 2Immunochemistry of prostaglandin endoperoxide-forming cyclooxygenases: The detection of the cyclooxygenases in rat, rabbit, and guinea pig kidneys by immunofluorescenceProstaglandins, Vol. 13, No. 5The role of the collecting duct in the regulation of excretion of sodium and other electrolytesKidney International, Vol. 6, No. 1Effect of plasma sodium concentration on diluting segment sodium reabsorptionKidney International, Vol. 5, No. 1 More from this issue > Volume 223Issue 4October 1972Pages 898-902 Copyright & PermissionsCopyright © 1972 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1972.223.4.898PubMed5075165History Published online 1 October 1972 Published in print 1 October 1972 Metrics

Renal response to blood volume expansion: distal tubular function and urinary excretion.

ARTICLESRenal response to blood volume expansion: distal tubular function and urinary excretionH SonnenbergH SonnenbergPublished Online:01 Oct 1972https://doi.org/10.1152/ajplegacy.1972.223.4.916MoreSectionsPDF (2 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByDominant factors that govern pressure natriuresis in diuresis and antidiuresis: a mathematical modelRobert Moss, and Anita T. Layton1 May 2014 | American Journal of Physiology-Renal Physiology, Vol. 306, No. 9Renal fluid and electrolyte handling in BKCa-β1−/− miceJennifer L. Pluznick, Peilin Wei, Pamela K. Carmines, and Steven C. Sansom1 June 2003 | American Journal of Physiology-Renal Physiology, Vol. 284, No. 6Renal regulation of salt balance: a primer for non-puristsPediatric Nephrology, Vol. 4, No. 4Chapter 25. Endogenous Natriuretic AgentsProstaglandin synthesis inhibition during volume expansion: Collecting duct functionKidney International, Vol. 22, No. 1CHLORPROMAZINE IN PROPHYLAXIS OF MYOCARDIAL INFARCTIONThe Lancet, Vol. 313, No. 8131CHLORIURETIC OR NATRIURETIC HORMONE?The Lancet, Vol. 313, No. 8131BLOOD-CULTURE TECHNIQUE: TWO NEEDLES OR ONE?The Lancet, Vol. 313, No. 8131Sodium wasting, acidosis and hyperkalemia induced by methicillin interstitial nephritisThe American Journal of Medicine, Vol. 64, No. 3Effects of furosemide, acetazolamide, and mannitol on medullary collecting-duct function in the rat kidneyPfl�gers Archiv European Journal of Physiology, Vol. 373, No. 2The Collecting DuctCollecting duct function in deoxycorticosterone acetate-escaped, normal, and salt-deprived rats. Response to hypervolemia.Circulation Research, Vol. 39, No. 2Effects of Diuretics on Renal Transport of PotassiumFiltration of protein in the anti-glomerular basement membrane nephritic rat: A micropuncture studyKidney International, Vol. 10, No. 6The effects of ringer-locke or blood infusions on the lateral intercellular spaces of the rat proximal tubuleThe Anatomical Record, Vol. 182, No. 2Neurosecretion and sodium excretionKidney International, Vol. 6, No. 5The role of the collecting duct in the regulation of excretion of sodium and other electrolytesKidney International, Vol. 6, No. 1Natriuretic HormonesEffect of plasma sodium concentration on diluting segment sodium reabsorptionKidney International, Vol. 5, No. 1 More from this issue > Volume 223Issue 4October 1972Pages 916-924 Copyright & PermissionsCopyright © 1972 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1972.223.4.916PubMed5075167History Published online 1 October 1972 Published in print 1 October 1972 Metrics

Pressures in static and dynamic states from capsules implanted in the kidney.

ARTICLESPressures in static and dynamic states from capsules implanted in the kidneyCE Ott, LG Navar, and AC GuytonCE Ott, LG Navar, and AC GuytonPublished Online:01 Aug 1971https://doi.org/10.1152/ajplegacy.1971.221.2.394MoreSectionsPDF (2 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByAssociations of renal sinus fat with blood pressure and ectopic fat in a diverse cohort of adultsInternational Journal of Cardiology Cardiovascular Risk and Prevention, Vol. 16The influence of systemic and ectopic fat depots on the state of kidney function3 January 2023 | Systemic Hypertension, Vol. 19, No. 4Altered renal medullary blood flow: A key factor or a parallel event in control of sodium excretion and blood pressure?7 April 2020 | Clinical and Experimental Pharmacology and Physiology, Vol. 47, No. 8Volumetric evaluation of renal sinus adipose tissue on computed tomography images in bilateral nephrolithiasis patients31 January 2020 | International Urology and Nephrology, Vol. 52, No. 6Renal hemodynamics and fatty acid uptake: effects of obesity and weight lossEleni Rebelos, Prince Dadson, Vesa Oikonen, Hidehiro Iida, Jarna C. Hannukainen, Patricia Iozzo, Ele Ferrannini,* and Pirjo Nuutila*5 November 2019 | American Journal of Physiology-Endocrinology and Metabolism, Vol. 317, No. 5Changes of renal sinus fat and renal parenchymal fat during an 18-month randomized weight loss trialClinical Nutrition, Vol. 37, No. 4Therapeutic effect of quercetin on renal function and tissue damage in the obesity induced ratsBiomedicine & Pharmacotherapy, Vol. 89Renal Autoregulation in Health and DiseaseMattias Carlström, Christopher S. Wilcox, and William J. Arendshorst1 April 2015 | Physiological Reviews, Vol. 95, No. 2Inhibition of sodium-linked glucose reabsorption normalizes diabetes-induced glomerular hyperfiltration in conscious adenosine A 1 -receptor deficient mice27 August 2013 | Acta Physiologica, Vol. 210, No. 2Scoping review report: obesity in older adults13 March 2012 | International Journal of Obesity, Vol. 36, No. 9Renal Medullary Circulation1 January 2012Fatty Kidney, Hypertension, and Chronic Kidney DiseaseHypertension, Vol. 58, No. 5Renal Sinus Fat and Poor Blood Pressure Control in Middle-Aged and Elderly Individuals at Risk for Cardiovascular EventsHypertension, Vol. 56, No. 5Moment-to-Moment Characteristics of the Relationship Between Arterial Pressure and Renal Interstitial Hydrostatic PressureHypertension, Vol. 56, No. 4The Renal Microcirculation1 January 2011The Renal MicrocirculationEctopic fat storage in heart, blood vessels and kidneys in the pathogenesis of cardiovascular diseases13 December 2004 | International Journal of Obesity, Vol. 28, No. S4The renin-angiotensin system in kidney developmentActa Physiologica Scandinavica, Vol. 181, No. 4Analysis of myogenic mechanisms in renal autoregulation9 April 2002 | Acta Physiologica Scandinavica, Vol. 174, No. 4Mechanisms of pressure natriuresisCurrent Hypertension Reports, Vol. 4, No. 2Mannitol therapy revisited (1940–1997)Kidney International, Vol. 52, No. 4Osmotic Diuretics: MannitolControl of Sodium Excretion: An Integrative Approach1 January 2011RENAL VENOUS WEDGE PRESSURE IN RENAL WRAP HYPERTENSION IN RABBITSClinical and Experimental Pharmacology and Physiology, Vol. 16, No. 8Role of prostaglandins in proximal tubule sodium reabsorption: response to elevated renal interstitial hydrostatic pressure.Circulation Research, Vol. 64, No. 5Escape from the sodium-retaining effects of mineralocorticoids: Role of ANF and intrarenal hormone systemsKidney International, Vol. 35, No. 3Pressure-diuresis in volume-expanded rats. Cortical and medullary hemodynamics.Hypertension, Vol. 12, No. 2Microcirculation of the KidneysA mathematical model of fluid transport in the kidneyActa Physiologica Scandinavica, Vol. 112, No. 4Progress toward resolving the controversy of positive Vs. negative interstitial fluid pressure.Circulation Research, Vol. 49, No. 2Stimulus-secretion coupling of renin. Role of hemodynamic and other factors.Circulation Research, Vol. 47, No. 4Influence of replacement of chloride by sulphate upon urine excretion and glomerular filtration rate in blood perfused isolated dog kidneysPflügers Archiv - European Journal of Physiology, Vol. 382, No. 3Transsinusoidal fluid dynamics in canine liver during venous hypertension.Circulation Research, Vol. 45, No. 3Effects of furosemide, acetazolamide, and mannitol on medullary collecting-duct function in the rat kidneyPfl�gers Archiv European Journal of Physiology, Vol. 373, No. 2Microcirculation and Tubular Urine Flow in the Mammalian Kidney Cortex (in vivo Microscopy)Changes in interstitial pressure during and after blood volume expansion in ratsPfl�gers Archiv European Journal of Physiology, Vol. 364, No. 3Influence of Peritubular Hydrostatic and Oncotic Pressures on Fluid Reabsorption in Proximal Tubules of the Rat KidneyActa Physiologica Scandinavica, Vol. 93, No. 2Role of physical and neuroendocrine factors in proximal electrolyte reabsorptionMetabolism, Vol. 23, No. 8Analysis of the Macula Densa Feedback Hypothesis for Intrinsic Regulation of Renal Vascular ResistanceIFAC Proceedings Volumes, Vol. 6, No. 4Transcapillary Fluid Exchange in the Renal CortexCirculation Research, Vol. 33, No. 1Colloid osmotic pressure of the subcapsular interstitial fluid of rat kidneys during hydropenia and volume expansionPfl�gers Archiv European Journal of Physiology, Vol. 340, No. 2 More from this issue > Volume 221Issue 2August 1971Pages 394-400 Copyright & PermissionsCopyright © 1971 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1971.221.2.394PubMed5560286History Published online 1 August 1971 Published in print 1 August 1971 Metrics

Effect of amiloride, ouabain, and furosemide on distal tubular function in the rat

DUARTE, CRISTOBAL G., FRANCOISE CHOMETY, AND GERHARD GIEBISCH. Effects of amiloride, ouabain, and furosemide on distal tubular function in the rat. Am. J. Physiol. 221(Z) : 632-639. 1971 .-Freeflow samples of tubular fluid (TF) were collected from superficial distal rat tubules and analyzed for inulin-14C, sodium (Na), and potassium (K). The effects of the three diuretic agents, a,miloride, ouabain, and furosemide, were studied. Amiloride completely suppressed K secretion at the distal tubular level with only small modifications of distal tubular Na reabsorption. Ouabain induced distal kaliuresis and natriuresis in animals kept on a low-potassium diet. The effects of ouabain upon distal tubular electrolyte transport consisted in depression of both tubular Na and K reabsorption. The distal potassium effects of ouabain were independent of any increase in distal sodium delivery and are thought to be due to depression of active potassium uptake across the luminal cell membrane of distal tubule cells. The main kaliuretic effect of furosemide on distal potassium transport is related to the delivery to the distal tubule of a much greater than normal fraction of fluid. After furosemide administration, despite a very significant elevation of distal tubular sodium concentration, fractional Na reabsorption rate control values. along this tubular segment greatly exceeded

Effect of diuretic states on hamster collecting duct electrical potential differences.

ARTICLESEffect of diuretic states on hamster collecting duct electrical potential differencesR Laurence, and DJ MarshR Laurence, and DJ MarshPublished Online:01 Jun 1971https://doi.org/10.1152/ajplegacy.1971.220.6.1610MoreSectionsPDF (2 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByElectrophysiological study of inner medullary collecting duct of hamstersPfl�gers Archiv European Journal of Physiology, Vol. 416, No. 1-2The role of the collecting duct in urinary concentrationKidney International, Vol. 31, No. 2Water, urea, sodium, chloride, and potassium transport in the in vitro isolated perfused papillary collecting ductKidney International, Vol. 22, No. 5MECHANISM OF CHLORIDE TRANSPORT ACROSS MAMMALIAN COLLECTING DUCTEffect of aldosterone on the transepithelial potential difference of the rat distal tubuleKidney International, Vol. 19, No. 5Electrophysiology of the early distal tubule: Further observations on electrode techniquesKidney International, Vol. 19, No. 1Structural-functional heterogeneity along the rabbit collecting tubuleKidney International, Vol. 14, No. 6Comparison between the electrical potential profile and the chloride gradients in the thin limbs of Henle's loop in ratsKidney International, Vol. 14, No. 5Effects of furosemide, acetazolamide, and mannitol on medullary collecting-duct function in the rat kidneyPfl�gers Archiv European Journal of Physiology, Vol. 373, No. 2The Collecting DuctElectrophysiological Measurements on the Renal TubuleSome aspects of distal tubular solute and water transportKidney International, Vol. 9, No. 2The effect of amiloride on the transepithelial potential difference of the distal tubule of the rat kidneyPfl�gers Archiv European Journal of Physiology, Vol. 361, No. 3Electrical properties of the medullary collecting ducts of the golden hamster kidneyPfl�gers Archiv European Journal of Physiology, Vol. 351, No. 2 More from this issue > Volume 220Issue 6June 1971Pages 1610-1616 Copyright & PermissionsCopyright © 1971 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1971.220.6.1610PubMed5087808History Published online 1 June 1971 Published in print 1 June 1971 Metrics

Influence of postglomerular hematocrit and protein concentration on rat nephron fluid transfer.

Influence of postglomerular hematocrit and protein concentration on rat nephron fluid transfer.

Acetazolamide in studying sodium reabsorption in diluting segment.

ARTICLESAcetazolamide in studying sodium reabsorption in diluting segmentJM Rosin, MA Katz, Rector FC Jr, and DW SeldinJM Rosin, MA Katz, Rector FC Jr, and DW SeldinPublished Online:01 Dec 1970https://doi.org/10.1152/ajplegacy.1970.219.6.1731MoreSectionsPDF (1 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByDonald Wayne Seldin, MD: A Conversation with the Editor11 December 2017 | Baylor University Medical Center Proceedings, Vol. 16, No. 2Pharmacology of DiureticsThe American Journal of the Medical Sciences, Vol. 319, No. 1Guidelines for Development of a New Diuretic AgentClearance Techniques1 January 2011Effects of Diuretics on Renal Function1 January 2011A lithium clearance study of sodium reabsorption at the proximal tubule in liver cirrhosis with ascitesGastroenterologia Japonica, Vol. 24, No. 5Reduction of renal blood flow and proximal bicarbonate reabsorption in rats by gentamicinKidney International, Vol. 24, No. 5Evidence for feedback mediated reduction of glomerular filtration rate during infusion of acetazolamideActa Physiologica Scandinavica, Vol. 114, No. 1Effect of hypochloremia on loop segment chloride and solute reabsorption in the rat during volume expansionKidney International, Vol. 20, No. 5SECRETION AND REABSORPTION OF BICARBONATE IN SINGLE RENAL TUBULES OF THE RABBITAnnals of the New York Academy of Sciences, Vol. 341, No. 1 Anion and ProRole of Extracellular Fluid Volume Expansion and Diuretics in Renal Handling of PhosphateInfluence of plasma potassium concentration on the capacity for sodium reabsorption in the diluting segment of the kidney8 July 2009 | Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 40, No. 1Control of Renal Bicarbonate Transport9 July 2009 | Membrane Biochemistry, Vol. 2, No. 3-4Effects of furosemide, acetazolamide, and mannitol on medullary collecting-duct function in the rat kidneyPfl�gers Archiv European Journal of Physiology, Vol. 373, No. 2Cellular Defects in Urinary Acidification and Renal Tubular AcidosisCharacteristics of transcellular NaCl reabsorption in the kidney8 July 2009 | Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 38, No. 7Renal energy metabolism and regulation of sodium reabsorptionKidney International, Vol. 11, No. 3Influence of volume expansion on NaCl reabsorption in the diluting segments of the nephron: A study using clearance methodsKidney International, Vol. 10, No. 3Some aspects of distal tubular solute and water transportKidney International, Vol. 9, No. 2The Use of Clearance Methods for the Determination of Sites of Action of Diuretics in the KidneyEffect of Acetazolamide on Glomerulotubular Balance and Renal Metabolic Rate8 July 2009 | Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 36, No. 7Effect of plasma sodium concentration on diluting segment sodium reabsorptionKidney International, Vol. 5, No. 1The influence of flow rate and chloride delivery on TcHO formation in the ratKidney International, Vol. 3, No. 5Alterations in renal tubular sodium and water reabsorption in chronic renal disease in manKidney International, Vol. 2, No. 3Renal acidosisKidney International, Vol. 1, No. 5Chapter 10. Diuretics More from this issue > Volume 219Issue 6December 1970Pages 1731-1738 Copyright & PermissionsCopyright © 1970 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1970.219.6.1731PubMed5485691History Published online 1 December 1970 Published in print 1 December 1970 Metrics

Renal tubular transfer of water and electrolytes in adrenalectomized rats.

ARTICLESRenal tubular transfer of water and electrolytes in adrenalectomized ratsMA CortneyMA CortneyPublished Online:01 Mar 1969https://doi.org/10.1152/ajplegacy.1969.216.3.589MoreSectionsPDF (2 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByRegulation of K+ ExcretionRegulation of Potassium ExcretionEffects of glucocorticoid and mineralocorticoid on potassium transport in the rat medullary thick ascending limb of Henle's loopKidney International, Vol. 47, No. 3The Effects of Oral Chloroquine Administration on Kidney Function7 July 2009 | Renal Failure, Vol. 16, No. 2A method to extend the application of microdroplet techniques in the SEM2 August 2011 | Journal of Microscopy, Vol. 170, No. 2Application of the Microdroplet Method of X-Ray Analysis to the Characterization of InksJournal of Forensic Sciences, Vol. 38, No. 1Transport Functions of the Distal Convoluted TubuleTransport Functions of the Distal Convoluted TubuleTubular Processing of Glomerular Ultrafiltrate: Mechanisms of Electrolyte and Water TransportElectron Probe Microanalysis for Clinical Investigations: Microdrop and Soft Tissue AnalysisAnnals of the New York Academy of Sciences, Vol. 428, No. 1 Technology ImDie Pathogenese des kardialen ÖdemsMechanism of increased renin release in the adrenalectomized rat. Adrenal insufficiency and renin.Hypertension, Vol. 5, No. 2_pt_2Factors influencing renal sodium reabsorption in volume expansion20 June 2005Heterogeneity of the rabbit collecting tubule: Localization of mineralocorticoid hormone action to the cortical portionKidney International, Vol. 20, No. 3Electron probe analysis of microdroplets: Factors affecting the proportionality between measured X-ray intensity and concentration2 August 2011 | Journal of Microscopy, Vol. 123, No. 3Proximal tubular reabsorption of inorganic phosphate in adrenalectomized ratsPfl�gers Archiv European Journal of Physiology, Vol. 385, No. 3Effect of insulinopenia and adrenal hormone deficiency on acute potassium toleranceKidney International, Vol. 17, No. 5Liquid Droplets and Isolated CellsTransport Functions of the Distal Convoluted TubuleSegmental heterogeneity of epithelial transport in rat large intestinePfl�gers Archiv European Journal of Physiology, Vol. 378, No. 1Effect of adrenalectomy on medullary collecting-duct function in rats before and during blood volume expansionPfl�gers Archiv European Journal of Physiology, Vol. 368, No. 1-2Elementary Quantitative Analysis of Lyophilized 10 -10 L Volume Solutions18 June 2020 | Proceedings, annual meeting, Electron Microscopy Society of America, Vol. 35Some aspects of distal tubular solute and water transportKidney International, Vol. 9, No. 2The mechanism of aldosterone functionPharmacology & Therapeutics. Part B: General and Systematic Pharmacology, Vol. 2, No. 2The role of bicarbonate in proximal tubular sodium chloride transportKidney International, Vol. 5, No. 4Effects of Aldosterone on Blood Pressure, Water, and ElectrolytesMechanism of sodium and water retention in rats with experimental heart failureKidney International, Vol. 4, No. 5Simultaneous Mg, Ca, P, K, Na and Cl analysis in rat tubular fluidPflügers Archiv, Vol. 340, No. 3Mechanism of action of aldosterone on potassium transfer in the rat kidneyPfl�gers Archiv European Journal of Physiology, Vol. 345, No. 2The tissue effects of mineralocorticoidsThe American Journal of Medicine, Vol. 53, No. 5Effect of aldosterone on sodium and potassium transport in the kidneyJournal of Steroid Biochemistry, Vol. 3, No. 2Time course of development of transtubular sodium concentration differences in proximal surface tubules of the rat kidneyPfl�gers Archiv European Journal of Physiology, Vol. 313, No. 3 More from this issue > Volume 216Issue 3March 1969Pages 589-598 Copyright & PermissionsCopyright © 1969 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1969.216.3.589PubMed5765611History Published online 1 March 1969 Published in print 1 March 1969 Metrics