Measurement Of Effects Research Articles

Advances in X-ray radiography for the measurement of dynamic effects in structured packings

Imaging techniques have significantly improved the understanding of fluid dynamics in mass transfer columns over the last decades. In order to detect small-scale and highly dynamic flow phenomena such as droplets, a high spatial and temporal resolution is of crucial importance. One approach is ultrafast electron beam X-ray computed tomography, another is to extend the X-ray computed tomography by a radiographic measuring method. The latter allows a very high spatial and temporal resolution. For example, the X-ray computed tomograph used in the present study allows up to 500projections per second. This enables the precise detection of highly dynamical effects in the entire column without influencing the flow. A new evaluation routine for radiographic measurements is presented and validated. The procedure enables to measure the liquid hold-up over a wide range with considerable low evaluation times, thus enabling a high experimental throughput. Additionally, a novel method to extract and quantify the dynamic share of the total hold-up is introduced. The possibilities arising from this are then explored utilizing Sulzeŕs structured packing MellapakTM as an example. We compare our findings of the hold-up with published data and discuss how the highly dynamic phenomena affect total flow, its local distribution, and substance dependency. Furthermore, the observations serve as indicator to which extend the computer tomographic method is suited to assess the total hold-up within a structured packing.

Theoretical Measurements of Quantitative Effects Caused by Spectator Ligands on Palladium-catalyzed C–H Activation

Ligands can definitely influence C–H activation at the metal center. A ligand not directly participating in the reaction is called a spectator ligand. We attempt to quantitatively characterize the effects of diverse spectator ligands on C-H activation at palladium. We designed a model palladium catalyst and selected an array of spectator ligands, such as methoxyl, amide, methyl, phenyl, cyanide, fluorine, chlorine, and several neutral ligands, and performed density functional theory calculations on the mechanism and energetics of C–H activation reactions of benzene with different catalysts. Univalent ligands have substantially larger effects than neutral ligands, and strongly σ-donating ligands (e.g., methyl and phenyl) severely hinder the C-H activation in progress. A ligand trans to the reaction site influences C–H activation more than that cis to the reaction site, indicating electronic effects to be at work. For example, the existence of a methyl ligand raises the barrier height of C-H activation by 6.4 or 14.4 kcal/mol when it is placed at the position cis or trans to the C–H activation site. The effects of poorly σ-donating ligands are not significant and similar to those of the κ1-acetate ligand. Some σ-donating and π-accepting ligands, such as cyanide and isonitrile, hinder the C–H activation trans to them but appear to facilitate the C–H activation cis to them. On the basis of molecular orbital analyses, a chemical model is proposed to understand the observed ligand effects. Lastly, the conclusions are applied to explain the plausible mechanism of the dehydrogenative Heck coupling.

Study on the exciton dynamic processes of exciplex host–guest system by transient magnetic field effects

Utilizing exciplex as the host and fluorescence emitter with dopant materials has been proved successfully to fabricate highly-efficient organic light-emitting diodes. Exciton evolution and energy transfer in this exciplex host–guest system are complex. Gaining insight into the electroluminescence (EL) mechanisms in exciplex-based devices is key for further optimizing device configuration. Here, we have investigated exciton dynamics in devices with exciplex as host and 4-(dicyanomethylene)-2-tert-butyl-6-(1,1,7,7-tetramethyljulolidin-4-yl-vinyl)-4H-pyran (DCJTB) as red fluorescence emitter. Two exciplexes, 2,6-bis(3-(9H-carbazol-9-yl)phenyl)pyridine (26DCzPPY) doped 2,4,6-tris[3-(triphenylphosphine)phenyl]-1,3,5-triazine (POT2T), and 4,4′,4″-tris[3-methylphenyl(phenyl)amino]-triphenylamine (m-MTDATA) doped tris(8-hydroxyquinoline)aluminum(III) (Alq3), with different band energy are utilized as host materials. Combining the measurements of transient EL, transient photoluminescence and magnetic field effect (MFE), it is concluded that Dexter energy transfer, together with Förster resonance energy transfer, are confirmed in the pure fluorescence doped system. Meanwhile, it is found that DCJTB works with the hot excitons mechanism but not a traditional red fluorescence emitter as recognized previously. This work presents that the transient MFE is powerful for detecting excitonic dynamic processes in excipelx based host–guest EL systems.

Construction of sandwich-structured Al/Mg-Li/Al multifunctional composites with enhanced electromagnetic shielding effectiveness performance

Magnesium alloy has good electromagnetic shielding performance, but its shielding effectiveness on high-frequency electromagnetic waves is weak. In order to solve the above problems, aluminum, which is also a lightweight material, is covered on top of Mg. The feasibility of this method is demonstrated by microstructure analysis and measurement of conductivity and electromagnetic shielding effect, and the results show that the Al/Mg-Li/Al-X sandwich structure is improved by nearly 10 dB at 6000 MHz, and the improvement of electromagnetic shielding efficiency is attributed to the absorption loss caused by the addition of Al layer and the reflection loss at the interlayer interfaces. In addition, the alternating arrangement of α-Mg/β-Li in the Mg layer, and the interfaces formed by the Mg12YZn hard phase particles and the matrix phase also contribute to the electromagnetic shielding efficiency.

Detecting GPCR Signals With Optical Biosensors of Gα-GTP in Cell Lines and Primary Cell Cultures.

G protein-coupled receptors (GPCRs) are the largest class of transmembrane receptors and mediate a wide variety of physiological processes. GPCRs respond to a plethora of extracellular ligands and initiate signaling pathways inside cells via heterotrimeric G proteins (Gαβγ). Because of the critical role GPCRs play in regulating biological processes and as pharmacological targets, the availability of tools to measure their signaling activity are of high interest. Live-cell biosensors that detect the activity of G proteins in response to GPCR stimulation have emerged as a powerful approach to investigate GPCR/G protein signaling. Here, we detail methods to monitor G protein activity through direct measurement of GTP-bound Gα subunits using optical biosensors based on bioluminescence resonance energy transfer (BRET). More specifically, this article describes the use of two types of complementary biosensors. The first protocol explains how to use a multicomponent BRET biosensor that relies on expression of exogenous G proteins in cell lines. This protocol yields robust responses that are compatible with endpoint measurements of dose-dependent ligand effects or with kinetic measurements of subsecond resolution. The second protocol describes the implementation of unimolecular biosensors that detect the activation of endogenous G proteins in cell lines expressing exogenous GPCRs or in primary cells upon stimulation of endogenous GPCRs. Overall, using the biosensors as described in this article will help users characterize the mechanisms of action of many pharmacological agents and natural ligands that modulate GPCR and G protein signaling with high precision. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Using bimolecular BRET biosensors to monitor Gα-GTP formation of tagged Gα in live cells Alternate Protocol 1: Measuring GPCR dose-dependent Gα-GTP responses in endpoint format Basic Protocol 2: Using unimolecular BRET biosensors to study endogenous G protein activity Alternate Protocol 2: Using unimolecular BRET biosensors to study endogenous G protein activity in mouse cortical neurons.

The Ferris ferromagnetic resonance technique: Principles and applications

Measurements of ferromagnetic resonance (FMR) are pivotal to modern magnetism and spintronics. Recently, we reported on the Ferris FMR technique, which relies on large-amplitude modulation of the externally applied magnetic field. It was shown to benefit from high sensitivity while being broadband. The Ferris FMR also expanded the resonance linewidth such that the sensitivity to spin currents was enhanced as well. Eventually, the spin Hall angle (θSH) was measurable even in wafer-level measurements that require low current densities to reduce the Joule heating. Despite the various advantages, analysis of the Ferris FMR response is limited to numerical modeling, where the linewidth depends on multiple factors such as the field modulation profile and the magnetization saturation. Here, we describe, in detail, the basic principles of operation of the Ferris FMR and discuss its applicability and engineering considerations. We demonstrated these principles in a measurement of the orbital Hall effect taking place in Cu using an Au layer as the orbital-to-spin current converter. This illustrates the potential of the Ferris FMR for the future development of spintronics technology.

A new method for characterizing piezoelectric properties of hexagonal boron nitride nanoflakes activated by focused ultrasound

Piezoelectric nanomaterials wirelessly activated by ultrasound have been studied for biomedical applications. However, the quantitative measurement of piezoelectric effects in nanomaterials and the correlation between the ultrasound dose and the piezoelectric amplitude are still explored. We demonstrated the synthesis of boron nitride nanoflakes by mechanochemical exfoliation and employed the electrochemical method to quantitatively evaluate the piezoelectric performance of the nanoflakes under ultrasonic circumstances. The change of voltametric charge, current, and voltage in response to different acoustic pressure was obtained in the electrochemical system. The charge was reached up to 69.29 μC with a net increase of 49.54 μC/mm2 under 2.976 MPa. The output current was measured up to 597 pA/mm2 and positive shift of output voltage shifted from −600 mV to −450 mV. Additionally, the piezoelectric performance linearly increased with acoustic pressure. The proposed method could be a standardized evaluation test bench for characterization of ultrasound-mediated piezoelectric nanomaterials.

Symmetry-based requirement for the measurement of electrical and thermal Hall conductivity under an in-plane magnetic field

The in-plane (thermal) Hall effect is an unconventional transverse response when the applied magnetic field is in the (heat) current plane. In contrast to the normal Hall effect, the in-plane Hall effect requires the absence of certain crystal symmetries, and possibly manifests a non-trivial topology of quantum materials. An accurate estimation of the intrinsic in-plane (thermal) Hall conductivity is crucial to identify the underlying mechanisms as in the case of the Kitaev spin-liquid candidate alpha-RuCl3. Here, we give the symmetry conditions for the in-plane Hall effect and discuss the implications that may impede the experimental evaluation of the in-plane (thermal) Hall conductivity within the single-device measurement. First, the lack of symmetry in crystals can create merohedral twin domains that cancel the total Hall signal. Second, even in a twin-free crystal, the intrinsic response is potentially contaminated by the out-of-plane conduction in three-dimensional systems, which is systematically unavoidable in the in-plane Hall systems. Third, even in a quasi-two-dimensional system, the conversion of (thermal) resistivity, rho (lambda), to (thermal) conductivity, sigma (kappa) requires protocols beyond the widely-used simplified formula due to the lack of in-plane-rotational symmetry. In principle, two independent sample devices are necessary to accurately estimate the s_xy (k_xy). As a case study, we discuss the half-integer quantization of the in-plane thermal Hall effect in the spin-disordered state of alpha-RuCl3. For an accurate measurement of the thermal Hall effect, it is necessary to avoid crystals with the merohedral twins contributing oppositely to k_xy, while the out-of-plane transport may have a negligible effect. To deal with the field-induced rotational-symmetry breaking, we propose two symmetry-based protocols, improved single-device and two-device methods.

Manipulating tunnelling gateways in condensed phase isomerization

AbstractWhen a chemical reaction occurs via tunnelling, a simple mass‐dependence is expected, where substitution of atoms by heavier isotopes leads to a reduced reaction rate. However, as shown in a recent study of CO orientational isomerization at the NaCl(1 0 0) interface, the lightest isotopologues need not exhibit the fastest tunnelling; for the CO/NaCl system, the non‐monotonic mass‐dependence is understood through a new picture of condensed phase tunnelling where the overall rate is dominated by a few pairs of reactant/product states. These state‐pairs – termed quantum gateways – gain dynamical importance through accidentally enhanced tunnelling probabilities, facilitated by a confluence of the energetic landscape underlying the reaction as well as the phonon bath of the surrounding medium. Here, we explore gateway tunnelling through measurements of the kinetic isotope effect for CO isomerization in a monolayer buried by many layers of either CO or N2. With an N2 overlayer, tunnelling rates are accelerated for all four isotopologues (12C16O, 13C16O, 12C18O and 13C18O), but the degree of acceleration is isotopologue‐specific and non‐intuitively mass dependent. A one‐dimensional tunnelling model involving an Eckart barrier cannot capture this behaviour. This reflects how a modification of the potential energy surface moves states in and out of resonance, thereby changing which tunnelling gateways can be accessed in the isomerization reaction.Key points The paper describes new systems that showcase resonance‐enhanced condensed phase tunnelling. Condensed phase tunnelling as described in this work may have implications for astrochemistry. A previously hypothesized mechanism is subjected to subsequent experimental scrutiny – the hypothesis stands the test.

Tensoelectric properties irradiated by the fast electrons n-Ge single crystals

Tensoelectric properties of the uniaxially deformed along the crystallographic direction [100] n-Ge single crystals, irradiated by the flows of electrons , , and , with the energy of , were investigated. Mechanisms of changes in resistivity and mobility of current carriers under the uniaxial deformation for the studied n-Ge single crystals based on the measurements of tenso-Hall effect and infrared Fourier spectroscopy were established.The presence of tensoresistance for n-Ge single crystals, irradiated by the flow of the electrons , is associated with changes under the uniaxial pressure of electron concentration in the conduction band and holes in the valence band due to a decrease of the ionization energy of the deep level of the radiation defects which belongs to the complexes , at the uniaxial deformation. A significant increase of the effective mobility of carriers current in uniaxially deformed single crystals n-Ge is primarily related to the deforming redistribution of electrons between the branch of ‘light’ holes and the level . For single crystals n-Ge irradiated by the flows of electrons , which lead to the n-p conversion of the conductivity type of germanium, significant tensoresistance is observed only at the pressures . In this case, the resistivity of n-Ge decreases both due to an increase in the concentration of holes and their mobility under the deformation. Mobility of holes does not depend on deformation for uniaxial pressures , and a slight decrease in the resistivity of the researched single crystals n-Ge is caused by an increase in their concentration. Radiation-induced tensosensitivity at the room temperature for single crystals n-Ge can be used in technologies for creating uniaxial pressure sensors based on germanium. Obtained radiation effect of a significant increase in the mobility of current carriers for the uniaxially deformed n-Ge single crystals can find its practical application in the creation of ‘germanium’ channels of n-MOSFET and p-MOSFET transistors.

Resolving Deleterious and Near-Neutral Effects Requires Different Pooled Fitness Assay Designs.

Pooled sequencing-based fitness assays are a powerful and widely used approach to quantifying fitness of thousands of genetic variants in parallel. Despite the throughput of such assays, they are prone to biases in fitness estimates, and errors in measurements are typically larger for deleterious fitness effects, relative to neutral effects. In practice, designing pooled fitness assays involves tradeoffs between the number of timepoints, the sequencing depth, and other parameters to gain as much information as possible within a feasible experiment. Here, we combined simulations and reanalysis of an existing experimental dataset to explore how assay parameters impact measurements of near-neutral and deleterious fitness effects using a standard fitness estimator. We found that sequencing multiple timepoints at relatively modest depth improved estimates of near-neutral fitness effects, but systematically biased measurements of deleterious effects. We showed that a fixed total number of reads, deeper sequencing at fewer timepoints improved resolution of deleterious fitness effects. Our results highlight a tradeoff between measurement of deleterious and near-neutral effect sizes for a fixed amount of data and suggest that fitness assay design should be tuned for fitness effects that are relevant to the specific biological question.

100 Years of Land-Use and Land-Cover Data: What Has Been the Effect of Spatial Planning in Coastal Land-Use and Land-Cover Change?

The Sustainable Development Goals require us to rethink spatial planning policies’ effectiveness. This article proposes a reproducible method for assessing the effect of past planning practices and simulating future land-use and land-cover (LULC) changes with a Cellular Automata model. The originality of our approach is to systematically compare observed changes in LULC with the planning rules in force over almost a century of evolution. A quasi-exhaustive database was constructed at a very fine spatial resolution for the municipality of Portimão (Southern Portugal), including the location and changes of LULC categories, and the planning rules of the corresponding time period on nine dates between 1947 and 2018. The quantified measurement of the actual effect of planning rules enables us to identify other determinants of the evolution. Findings reveal that the policies established by the local government—which aimed to foster well-planned comprehensive urban areas—were not as effective as intended. The quantified discrepancies between planning recommendations and observed evolution help to simulate which LULC scenarios could be designed to reach the expected result in future planning policies. Our assessment method could be applied in other urban and tourist regions where land artificialization exerts strong pressure on the environment.

Measurement of Sprawl Effect Based on Urban Growth Trends and Prediction in Kedungkandang District, Malang City

Urban development generally produces a specific type of urban growth that can be identified by looking at the pattern formed by the growth of occuring built-up area. Urban growth in some theories has three types of growth, namely infill, leapfrog and sprawl. Each type has logical consequences for environmental sustainability, such as leapfrog and sprawl which can lead to inefficient use of resources. This study focuses on measuring sprawl intensity based on urban growth trends or built-up area in Kedungkandang District from 2012 to 2020 and based on the results of urban growth or built-up area predictions from 2021 to 2036. Urban growth predictions are carried out using the Cellular Automata method, that there will be 975 hectares built-up land growth from 2016 to 2036. Then to measure the intensity of sprawl as a result of urban growth, the Shannon’s Entropy method is used with the result that the urban growth process in Kedungkandang District within 24 years tends to decrease in sprawl intensity by 50%. The results of this study are expected to provide insight and future views for the Government in dealing with urban growth in Kedungkandang District in the future.

On-site measurement and numerical simulation study on characteristic of urban heat island in a multi-block region in Beijing, China

The urban heat island (UHI) effect can lead to an increase in winter temperatures in cold regions, which in turn increases energy consumption and air pollution within urban areas. Field measurements of UHI effects can provide high-precision and high-resolution data, making them suitable for investigating the spatial-temporal distribution of UHI. However, there have been relatively few studies on the field testing of UHI effects in multiple blocks, especially those that involve continuous monitoring. Additionally, while many studies have demonstrated the impact factors of UHI intensity, there is a scarcity of research on quantitative and weight analyses of these factors. In this study, on-site measurements of UHI characteristics were carried out in a typical multi-block region in Beijing. The on-site sampling was conducted in both summer and winter seasons for 29 consecutive sampling days with 20 sampling points. The average urban heat island intensity (UHII) during the sampling period was 1.7 ± 1.2 °C in summer and 3.7 ± 1.8 °C in winter. Temperatures measured at different sampling locations varied significantly, with a maximum spatial difference of 7.5 °C in summer and 4.1 °C in winter. Simulation results showed that, in summer, each ±10% change in wind speed, albedo, or solar radiation intensity would cause the UHII to change by ∓0.16 °C, ∓0.21 °C, or ± 0.17 °C, respectively. In winter, the effects of these factors were very limited. The effect weights of wind speed, albedo, and solar radiation intensity on UHII in summer were 31.3%, 36.6%, and 32.1%, respectively. The position of green space in relation to the dominant wind direction of the city would have a greater effect on UHI mitigation in summer than in winter. This study provides a theoretical basis for mitigating UHI effects and is of great significance for sustainable urban design.

Fatty Liver Disease, Metabolism and Alcohol Interplay: A Comprehensive Review.

Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease worldwide, and its incidence has been increasing in recent years because of the high prevalence of obesity and metabolic syndrome in the Western population. Alcohol-related liver disease (ArLD) is the most common cause of cirrhosis and constitutes the leading cause of cirrhosis-related deaths worldwide. Both NAFLD and ArLD constitute well-known causes of liver damage, with some similarities in their pathophysiology. For this reason, they can lead to the progression of liver disease, being responsible for a high proportion of liver-related events and liver-related deaths. Whether ArLD impacts the prognosis and progression of liver damage in patients with NAFLD is still a matter of debate. Nowadays, the synergistic deleterious effect of obesity and diabetes is clearly established in patients with ArLD and heavy alcohol consumption. However, it is still unknown whether low to moderate amounts of alcohol are good or bad for liver health. The measurement and identification of the possible synergistic deleterious effect of alcohol consumption in the assessment of patients with NAFLD is crucial for clinicians, since early intervention, advising abstinence and controlling cardiovascular risk factors would improve the prognosis of patients with both comorbidities. This article seeks to perform a comprehensive review of the pathophysiology of both disorders and measure the impact of alcohol consumption in patients with NAFLD.

Thinking teacher education through military imaginaries: A diffractive and decolonising reading

ABSTRACT This article problematises Initial Teacher Education (ITE) and assessment practices to contribute to the ongoing work of decolonising higher education. It critiques the entanglement of military imaginaries and ITE via a diffractive reading of ITE discourse and policy in the Australian context through military imaginaries in academic and SF literature. In military imaginaries there exists an imperative to enact warfare as ‘target processing’; that is, the essence of warfare is understood to be the operationalisation of a continuous cycle of identification of targets, selection and implementation of pre-determined methodologies to match these targets, followed by measurement of the outcomes of the process. This article considers how current ITE expectations prioritise a similar continuous cycle of selection and implementation of standardised methodologies and measurement of effects; effectively an ideology of ‘teaching as processing’. It examines how this expectation assists in creating certain concerning expectations and disappearances within teaching and education.

Destabilization mechanism of the collisional microtearing mode in magnetized slab plasmas

The destabilization mechanism of the collisional microtearing mode driven by an electron temperature gradient is studied using theoretical analyses and gyrokinetic simulations including a comprehensive collision model, in magnetized slab plasmas. The essential destabilization mechanism of the microtearing mode is the lag of the parallel inductive electric field behind the magnetic field owing to the time-dependent thermal force and inertia force induced by the velocity-dependent electron–ion collisions. Quantitative measurements of the collision effects enable us to identify the unstable regime against collisionality and reveal the relevance of the collisional microtearing mode with existing toroidal experiments. A nonlinear simulation demonstrates that the microtearing mode does not drive magnetic reconnection with the explosive release and conversion of the magnetic energy.

Evaluation and measurement of technological functions

Technological transfer is an important factor of technological development and requires assessment and measurement of effects. Evaluation and measurement can be done with the use of models, the most important being the model of the technology, i.e. of the object being transferred. In the paper, a version of technological function's model is developed, starting from the models of interaction, establishing links, choosing the mechanism and forming the interaction interface, forming the technological function by modifying the internal functions of the objects in interaction and modifying the properties them. The modifications of the properties of the object subjected to the action, of the object carrying technological tasks and of the object-interface serves as the basis for the evaluation and measurement of the technological function in all its complexity. It is shown that technological task bearers can be of two categories: physical-technical (materials, energy, processes, etc.) and immaterial (theories, concepts, data, knowledge, etc.). Both the mechanisms and the measure of manifestation of the technological functions for the two categories are different.

Measurement of Doppler effects in a cryogenic buffer-gas cell

Buffer-gas cooling is a universal cooling technique for molecules and used for various purposes. One of its applications is using molecules inside a buffer-gas cell for low-temperature spectroscopy. Although a high-intensity signal is expected in the cell, complex molecular dynamics is a drawback for precise spectroscopy. In this study, we performed high-resolution absorption spectroscopy of low-J transitions in the $\tilde{A}^2\Pi (0,0,0)-\tilde{X}^2\Sigma ^+(0,0,0)$ band of calcium monohydroxide (CaOH). CaOH molecules were produced by laser ablation in a copper cell and cooled to $\sim$5\,K using helium buffer gas. We probed the Doppler effects in a buffer-gas cell by injecting counter-propagating lasers inside the cell. The time evolutions of the Doppler width and shift were simulated using a dedicated Monte Carlo simulation and compared with data.

Abstract 5402: Using computer vision to resolve proliferative dynamics within therapeutic responses in large scale screens of patient derived models

Abstract IC50, Emax, and AUC values are commonly used to assess susceptibility for a given therapeutic candidate but are subject to the number of cell divisions for a given in vitro model. When comparing multiple in vitro models and especially patient-derived models such as patient-derived tumor organoids, the degree of intrinsic cell proliferation or doubling time substantially differs and therefore limits conventional metrics as above. Furthermore, assays that directly measure cellular viability can oftentimes overlook therapeutic agents that have cytostatic, versus cytotoxic mechanisms of action and require assays with similar sensitivity and precision to account for this phenotype. Finally, terminal endpoint assays can require tedious temporal optimization to capture the full dynamic range of a given therapeutic which makes cross-therapeutic and cross-model comparisons difficult to interpret. An assay that incorporates these multiple features and in vitro specific doubling time would lead to highly accurate measurements of drug effects and allow the calculation of growth-adjusted IC50 values i.e. GI50 values. We, therefore, sought to develop a computer vision model that utilizes label-free longitudinal light microscopy images as input to report the total number of nuclei present within a given experimental well, allowing for the monitoring of cell division and any cytostatic effects. To train the neural network, we use Hoechst-stained images as our ground truth. The model used is an extension of the Regularized Conditional Adversarial Network (PMID: 34320344). It takes as input Brightfield images along with the registered Hoescht stained wells. The label for each well is obtained using commercial image processing software to count the total number of nuclei present in each well from the Hoescht stained channel. The model was trained to predict the virtually stained Hoescht well for every Brightfield, as well as the aggregate nuclei count for all organoids present inside the well. The model was trained on 29000 individual sites across 9 distinct cancer types. Five experiments were conducted during training to test for bit depth and tonality effects of the input training data. Inference on 9240 images showed a pearsonR score of 0.81 on the best-performing model. Results show that the model performs well and can be applied in real-time using only experimental data to correct for differing cell division rates and measure cytostatic phenotypes all while using simple light microscopy. The tool will enable cross-comparison of different therapeutic MoAs as well as enable cross-cancer type/indication comparison for a therapeutic in development to inform early development clinical strategy. Citation Format: Madhavi Kannan, Brian Larsen, Chi-Sing Ho, Jagadish Venkataraman, Martin Stumpe, Ameen Salahudeen. Using computer vision to resolve proliferative dynamics within therapeutic responses in large scale screens of patient derived models. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5402.