Mating Partners Research Articles

Chemical and Mechanical Signals Trigger Courtship in the Wild Large-Headed Resin Bee Heriades truncorum

Hymenopteran females are mostly highly selective, choosing their mating partner according to male quality signals, and may also signal their fertility through chemical advertisement. Male insects, in turn, often display a complex set of courtship behaviors to allure females to mate. In this study we explored the mechanisms triggering courtship behaviors in the oligolectic bee Heriades truncorum. In this species, males perform wing fanning followed by a sideways rocking motion before copulation. We tested the role of chemical cues in partner recognition by exposing males to dead females washed from cuticular extracts (i.e., sex pheromones) that were posteriorly coated, or not, with an extract of unmated females. Additionally, we experimentally reduced male wing length to test whether wing fanning was responsible for triggering sideways rocking motions. We found males to interact more often with females coated by extracts from unmated females, and to display wing fanning in the presence of these pheromones. Male wing fanning was important to perform sideways rocking motions, which are an essential behavior for successful mating and possibly an element used by females to evaluate male quality. Our study shows that both chemical and mechanical signals from female and male bees, respectively, are important elements in the mating system of H. truncorum.

Anidulafungin Treatment Blocks the Sexual Cycle of Pneumocystis murina and Prevents Growth and Survival without Rescue by an Alternative Mode of Replication.

The proposed life cycle of fungi in the genus Pneumocystis has typically included both an asexual cycle via binary fission and a sexual cycle. Until recently, the strategy used for sexual replication was largely unknown, but genomic and functional assays now support a mode known as primary homothallism (self-fertilization). The question of whether an asexual cycle contributes to the growth of these fungi remains. Treatment of Pneumocystis pneumonia in immunosuppressed rodent models with the class of drugs known as echinocandins is challenging the historical concept of asexual replication. The echinocandins target 1,3-β-D-glucan (BG) synthesis resulting in death for most fungi. Because Pneumocystis species have both non-BG expressing life cycle stages (trophic forms) and BG-expressing asci, treatment with anidulafungin and caspofungin resulted in elimination of asci, with large numbers of non-BG expressing organisms remaining in the lungs. Transcriptional analyses of anidulafungin treated Pneumocystis murina-infected lungs indicated that these agents were blocking the sexual cycle. In the present study, we explored whether there was an asexual or alternative method of replication that could rescue P. murina survival and growth in the context of anidulafungin treatment. The effects of anidulafungin treatment on early events in the sexual cycle were investigated by RT-qPCR targeting specific mating genes, including mam2, map3, matMi, matPi, and matMc. Results from the in vivo and gene expression studies clearly indicated there was no rescue by an asexual cycle, supporting these fungi's reliance on the sexual cycle for survival and growth. Dysregulation of mating-associated genes showed that anidulafungin induced effects early in the mating process. IMPORTANCE The concept of a sexually obligate fungus is unique among human fungal pathogens. This reliance can be exploited for drug development and here we show a proof of principle for this unusual target. Most human fungal pathogens eschew the mammalian environment with its battery of immune responses. Pneumocystis appear to have evolved to survive in such an environment, perhaps by using sexual replication to help in DNA repair and to introduce genetic variation in its major surface antigen family because the lung is the primary environment of these pathogens. The concept of primary homothallism fits well into its chosen ecosystem, with ready mating partners expressing both mating type receptors, and a sexual cycle that can introduce beneficial genetic variation without the need for outbreeding.

Squeeze-out and bond strength of patterned Cu[sbnd]Sn SLID seal-frames

Control of Sn squeeze-out due to the presence of liquid phase and bond pressure is important in CuSn SLID bonding. This work studies the impacts of seal-frame design for limiting this squeeze-out. Three alternative seal-frame geometries are evaluated and compared to a traditional continuous seal-frame with respect to squeeze-out and shear strength. The alternative designs include open spaces to accommodate squeeze-out and are: 1) One mating partner with 3 × 50 μm wide rails and 25 μm gaps. 2) Previous geometry with 50 μmx200 μm square hatches added across the rails with a pitch of 75 μm. 3) One 150 μm wide mating partner leaving 25 μm of open area at either side. For all geometries, the other mating partner is a continuous 200 μm wide seal-frame. A 46 % reduction in squeeze out could be seen in the bond with asymmetric bonding partners, but no significant reduction was measured for the geometry with rails and the geometry with hatches. The hatched, railed and asymmetric seal-frames displayed shear strengths of 57 ± 13 MPa, 80 ± 18 MPa, and 48 ± 30 MPa respectively, which was significantly higher than that of the traditional continuous bonds which only measured 17 ± 7 MPa. Fracture surface analysis showed no correlation between fracture modes and differences in shear strengths.

Genetic Association between Farrowing Rates and Swine Leukocyte Antigen Alleles or Haplotypes in Microminipigs.

We have previously reported specific swine leukocyte antigen (SLA) haplotype associations with significant effects on several reproduction performance traits in a highly inbred miniature pig population of Microminipigs (MMPs). In this study, to clarify the effects on farrowing rates of SLA similarity between mating partners in the MMP population, we compared the farrowing rates as a measure of reproductive success after 1063-cumulative matings among the following three groups of mating partners: (1) completely sharing SLA class I or class II haplotypes or alleles between partners (CS), (2) only one sharing the haplotypes or alleles (OS), and (3) non-sharing the haplotypes or alleles (NS). Average farrowing rates in CS groups consisting of completely sharing SLA class II haplotypes or DRBI and DQB1 alleles were lowest in the three groups. Moreover, lower farrowing rates were indicated in mating pairs with smaller amino acid pairwise genetic distances of SLA-1, SLA-3, DRB1 and DQB1 alleles between the pairs. These results suggested that the dissimilarity of SLA class I and class II alleles between mating partners markedly improved reproductive performance; therefore, SLA alleles or haplotypes are potentially useful genetic markers for the selection of mating pairs in breeding programs and epistatic studies of reproductive traits of MMPs.

Kinesin-2 motors differentially impact biogenesis of extracellular vesicle subpopulations shed from sensory cilia

SummaryExtracellular vesicles (EVs) are bioactive lipid-bilayer enclosed particles released from nearly all cells. One specialized site for EV shedding is the primary cilium. Here, we discover the conserved ion channel CLHM-1 as a ciliary EV cargo. Imaging of EVs released from sensory neuron cilia of Caenorhabditis elegans expressing fluorescently tagged CLHM-1 and TRP polycystin-2 channel PKD-2 shows enrichment of these cargoes in distinct EV subpopulations that are differentially shed in response to mating partner availability. PKD-2 alone is present in EVs shed from the cilium distal tip, whereas CLHM-1 EVs bud from a secondary site(s), including the ciliary base. Heterotrimeric and homodimeric kinesin-2 motors have discrete impacts on PKD-2 and CLHM-1 colocalization in both cilia and EVs. Total loss of kinesin-2 activity decreases shedding of PKD-2 but not CLHM-1 EVs. Our data demonstrate that anterograde intraflagellar transport is required for selective enrichment of protein cargoes into heterogeneous EVs with different signaling potentials.

Effects of social and environmental contexts on multi-male mating and mixed paternity in socially monogamous female prairie voles.

With whom and how often to mate are fundamental questions that impact individual reproductive success and the mating system. Relatively few studies have investigated female mating tactics compared with males. Here, we asked how differential access to mates influences the occurrence of mixed paternity and overall reproductive success in socially monogamous female prairie voles (Microtus ochrogaster). We created male- and female-biased sex ratios of prairie voles living in semi-natural outdoor enclosures. We ran paternity analyses to determine the identity and number of mating partners females had and the number of offspring produced. We found that 57.1% of females had litters fathered by two or more males when males outnumbered females, and 87.5% of females had litters with more than one father when females outnumbered males. However, the percentage of mixed paternity and the total number of embryos were not statistically different between social contexts. We determined that female fecundity (i.e. number of embryos) correlated with the number of male fathers in each litter across social contexts. Although our study did not support the hypothesis that social context directly influences female mating decisions, it did suggest that female multi-male mating might lead to increased fertilization success under semi-natural conditions.

Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery.

The mating of budding yeast depends on chemotropism, a fundamental cellular process. Haploid yeast cells of opposite mating type signal their positions to one another through mating pheromones. We have proposed a deterministic gradient sensing model that explains how these cells orient toward their mating partners. Using the cell-cycle determined default polarity site (DS), cells assemble a gradient tracking machine (GTM) composed of signaling, polarity, and trafficking proteins. After assembly, the GTM redistributes up the gradient, aligns with the pheromone source, and triggers polarized growth toward the partner. Since positive feedback mechanisms drive polarized growth at the DS, it is unclear how the GTM is released for tracking. What prevents the GTM from triggering polarized growth at the DS? Here, we describe two mechanisms that are essential for tracking: inactivation of the Ras GTPase Bud1 and positioning of actin-independent vesicle delivery upgradient.

Mating pair drives aggressive behavior in female Drosophila

Aggression is an adaptive set of behaviors that allows animals to compete against one another in an environment of limited resources. Typically, males fight for mates and food, whereas females fight for food and nest sites.1 Although the study of male aggression has been facilitated by the extravagant nature of the ritualized displays involved and the remarkable armaments sported by males of many species,2,3,4 the subtler and rarerinstances of inter-female aggression have historically received much less attention. In Drosophila, females display high levels of complex and highly structured aggression on a food patch with conspecific females.5,6,7,8,9 Other contexts of female aggression have not been explored. Indeed, whether females compete for mating partners, as males do, has remained unknown so far. In the present work, we report that Drosophila melanogaster females reliably display aggression toward mating pairs. This aggressive behavior is regulated by mating status and perception of mating opportunities and relies heavily on olfaction. Furthermore, we found that food odor in combination with OR47b-dependent fly odor sensing is required for proper expression of aggressive behavior. Taken together, we describe a social context linked to reproduction in which Drosophila females aspiring to mate produce consistent and stereotyped displays of aggression. These findings open the door for further inquiries into the neural mechanisms that govern this behavior.

Mate choice is affected by parasite infestation rate of the choosing individual as well as of potential mating partners.

Parasites are known to be a key driving force in mate choice and are important for the expression and evolution of ornaments and behavioral traits being used. However, there is little experimental evidence on how the parasite's burden of the choosing individual is integrated into the mate-choice process and how it affects decision-making, especially in relation to parasite infestation of potential mates. Thus, the aim of our study was to determine whether female house sparrows Passer domesticus adjust their mate preference according to their own as well as the parasite load of prospective partners. To do this, we experimentally manipulated female parasite load and determined their mate preferences prior to and after parasite treatment. We manipulated the chronic coccidian parasite burden of females either by initiating the acute infection phase via re-infecting them with coccidian or by temporally reducing the parasite load of coccidia. We then measured the effect of this manipulation on mate preference by presenting females with a choice of four stimuli: three males with similar ornaments, but unmanipulated, naturally varying chronic coccidiosis levels, and an unmanipulated control female. Additionally, we recorded some males' behavior in relation to their infection status pointing toward an increased or reduced interest in mating. We found that females preferred highly infested males prior to manipulation, regardless of their own infestation level. However, after manipulation, infested females avoided highly infested males probably in response to the deterioration of their health condition by parasites. Our study suggests that mate-choice decisions are more complex when they are mediated by parasites. The implications of parasites for evolutionary theories of sexual signaling and mate choice are discussed.

Mate choice in a promiscuous poison frog.

In many animal species, members of one sex, most often females, exhibit a strong preference for mating partners with particular traits or resources. However, when females sequentially mate with multiple partners, strategies underlying female choice are not very well understood. Particularly, little is known if under such sequential polyandry females mate truly randomly, or if they actively try to spread mating events across multiple partners. In the present study, we used the highly promiscuous poison frog Allobates femoralis to investigate whether promiscuity could result from a preference for novel mates. Furthermore, we examined the importance of call characteristics for mate choice. We conducted mate choice experiments in a laboratory setup, by presenting females with recent mating partners or novel males. We recorded call characteristics of both males and the time females spent close to each male. In our trials, females preferred previous mating partners over novel males and also males with shorter advertisement calls. Results from previous studies on A.femoralis suggest that females in our trials recognized previous partners based on individual call characteristics. While mating decisions in the wild and in the laboratory might differ, our study provides first evidence for female mate choice in a poison frog with sequential polyandry.

Genetic footprints of assortative mating in the Japanese population

Assortative mating (AM) is a pattern characterized by phenotypic similarities between mating partners. Detecting the evidence of AM has been challenging due to the lack of large-scale datasets that include phenotypic data on both partners, especially in populations of non-European ancestries. Gametic phase disequilibrium between trait-associated alleles is a signature of parental AM on a polygenic trait, which can be detected even without partner data. Here, using polygenic scores for 81 traits in the Japanese population using BioBank Japan Project genome-wide association studies data (n = 172,270), we found evidence of AM on the liability to type 2 diabetes and coronary artery disease, as well as on dietary habits. In cross-population comparison using United Kingdom Biobank data (n = 337,139) we found shared but heterogeneous impacts of AM between populations.

Hearing, touching, and multisensory integration during mate choice.

Mate choice is a potent generator of diversity and a fundamental pillar for sexual selection and evolution. Mate choice is a multistage affair, where complex sensory information and elaborate actions are used to identify, scrutinize, and evaluate potential mating partners. While widely accepted that communication during mate assessment relies on multimodal cues, most studies investigating the mechanisms controlling this fundamental behavior have restricted their focus to the dominant sensory modality used by the species under examination, such as vision in humans and smell in rodents. However, despite their undeniable importance for the initial recognition, attraction, and approach towards a potential mate, other modalities gain relevance as the interaction progresses, amongst which are touch and audition. In this review, we will: (1) focus on recent findings of how touch and audition can contribute to the evaluation and choice of mating partners, and (2) outline our current knowledge regarding the neuronal circuits processing touch and audition (amongst others) in the context of mate choice and ask (3) how these neural circuits are connected to areas that have been studied in the light of multisensory integration.

Functional characterization of sex pheromone receptors in Spodoptera frugiperda, S. exigua, and S. litura moths.

Moths possess an extremely sensitive and diverse sex pheromone processing system, in which pheromone receptors (PRs) are essential to ensure communication between mating partners. Functional properties of some PRs are conserved among species, which is important for reproduction. However, functional differentiation has occurred in some homologous PR genes, which may drive species divergence. Here, using genome analysis, 17 PR genes were identified from Spodoptera frugiperda, S. exigua, and S. litura, which belong to 6 homologous groups (odorant receptor [OR]6, 11, 13, 16, 56, and 62); of which 6 PR genes (OR6, OR11, OR13, OR16, OR56, and OR62) were identified in S. frugiperda and S. exigua, and 5 PR genes were identified in S. litura, excluding OR62. Using heterologous expression in Xenopus oocytes, we characterized the functions of PR orthologs including OR6, OR56, and OR62, which have not been clarified in previous studies. OR6 orthologs were specifically tuned to (Z,E)-9,12-tetradecadienyl acetate (Z9,E12-14:OAc), and OR62 orthologs were robustly tuned to Z7-12:OAc in S. frugiperda and S. exigua. The optimal ligand for OR56 was Z7-12:OAc in S. frugiperda, but responses were minimal in S. exigua and S. litura. In addition, SfruOR6 was male antennae-specific, whereas SfruOR56 and SfruOR62 were male antennae-biased. Our study further clarified the functional properties of PRs in 3 Spodoptera moth species, providing a comprehensive understanding of the mechanisms of intraspecific communication and interspecific isolation in Spodoptera.

Male mating preference in an ixodid tick

BackgroundMate choice is a fundamental element of sexual selection and has the potential to shape the evolution of traits. Mate choice based on body size has been shown to be a common trait in several arthropod species. In hard ticks, a taxon of medical and veterinary importance, engorgement weight is positively correlated with reproductive output but it is unknown whether adult males show mate choice. Here, we experimentally investigated whether males (i) use chemical cues to choose their mating partner, (ii) consistently choose for the same female individual and (iii) prefer females with highest weight after feeding.MethodsWe used two experimental setups which allowed chemical communication between ticks: (i) a horizontal tube preventing physical contact with the female and (ii) an arena where tactile cues were allowed. In total, we tested 62 different triads in 124 tests (66 tests in the horizontal tube and 58 in the arena) composed of one male that could choose between two engorged females. Specifically, we tested 42 triads in the tube and 46 in the arena; 24 triads were repeatedly tested in the tube while 38 triads were tested in both setups.ResultsWe found no preference for individual or heavier females in either setup. However, in the horizontal tube setup, males significantly preferred females that were not visited by them in the previous test.ConclusionsOur results suggest a lack of male mate choice despite heavier females having higher fecundity. However, future studies should take into account that males may recognize the potential mating partners they previously met.Graphical

Reprogramming the topology of the nociceptive circuit in C. elegans reshapes sexual behavior

The effect of the detailed connectivity of a neural circuit on its function and the resulting behavior of the organism is a key question in many neural systems. Here, we study the circuit for nociception in C.elegans, which is composed of the same neurons in the two sexes that are wired differently. We show that the nociceptive sensory neurons respond similarly in the two sexes, yet the animals display sexually dimorphic behaviors to the same aversive stimuli. To uncover the role of the downstream network topology in shaping behavior, we learn and simulate network models that replicate the observed dimorphic behaviors and use them to predict simple network rewirings that would switch behavior between the sexes. We then show experimentally that these subtle synaptic rewirings indeed flip behavior. Interestingly, when presented with aversive cues, rewired males were compromised in finding mating partners, suggesting that network topologies that enable efficient avoidance of noxious cues have a reproductive "cost." Our results present a deconstruction of the design of a neural circuit that controls sexual behavior and how to reprogram it.

Urban Individuals of Three Rove Beetle Species Are Not More Exploratory or Risk-Taking Than Rural Conspecifics.

Simple SummaryUrbanization-derived disturbances and threats, as well as changes in environmental and habitat parameters act as selection pressures on various features of urban-dwelling animals, including their behavior. Earlier studies on vertebrates showed that urban individuals are more exploratory and bolder than their rural counterparts. Similar analyses on invertebrates are rare, therefore we studied the exploratory and risk-taking behavior of individuals of three rove beetle species from rural and urban populations during their main reproductive period. Beetles of all three studied species responded consistently in the different behavioral tests. The exploratory behavior of beetles was consistent over time indicating the existence of personalities, but did not differ in differently urbanized habitats. Ocypus nitens males, however, were significantly more exploratory than females which can be explained by the active searching of males for mating partners.Urbanization is creating changes in environmental and habitat conditions, as well as creating disturbance and threats to urban-associated species. Some traits, such as high exploratory and risk-taking behavior, are beneficial to allow colonization of urban habitats and coping with urbanization-derived pressures. In this study the exploratory and risk-taking behavior of rural and urban individuals of three forest-associated rove beetle species were tested during their main reproductive period by five frequently used behavioral measures. Individuals of all studied species were similarly ranked by all behavioral measures, indicating that the studied rove beetles responded consistently in the different contexts. However, the behavior of beetles was consistent over time for all/most studied species only by using two measures of exploratory behavior. These provide evidence for the existence of the exploratory dimension of personality in rove beetles. We found a higher exploratory behavior in males than females in Ocypus nitens which can be explained by the active searching of males for mating partners. There were no urbanization-related differences in the exploratory behavior of individuals, suggesting that behavioral changes (being more exploratory) may not yield additional fitness benefits in these rove beetle species with good dispersal capacity.

Mechanism of commitment to a mating partner in Saccharomyces cerevisiae.

Many cells detect and follow gradients of chemical signals to perform their functions. Yeast cells use gradients of extracellular pheromones to locate mating partners, providing a tractable model for understanding how cells decode the spatial information in gradients. To mate, yeast cells must orient polarity toward the mating partner. Polarity sites are mobile, exploring the cell cortex until they reach the proper position, where they stop moving and "commit" to the partner. A simple model to explain commitment posits that a high concentration of pheromone is detected only upon alignment of partner cells' polarity sites and causes polarity site movement to stop. Here we explore how yeast cells respond to partners that make different amounts of pheromone. Commitment was surprisingly robust to various pheromone levels, ruling out the simple model. We also tested whether adaptive pathways were responsible for the robustness of commitment, but our results show that cells lacking those pathways were still able to accommodate changes in pheromone. To explain this robustness, we suggest that the steep pheromone gradients near each mating partner's polarity site trap the polarity site in place.

The need for unbiased genetic screens to dissect aggression in Drosophila melanogaster.

Aggression is an evolutionarily conserved behavior present in most animals and is necessary for survival when competing for limited resources and mating partners. Studies have shown that aggression is modulated both genetically and epigenetically, but details of how the molecular and cellular mechanisms interact to determine aggressive behavior remain to be elucidated. In recent decades, Drosophila melanogaster has emerged as a powerful model system to understand the mechanisms that regulate aggression. Surprisingly most of the findings discovered to date have not come from genetic screens despite the fly’s long and successful history of using screens to unravel its biology. Here, we highlight the tools and techniques used to successfully screen for aggression-linked behavioral elements in Drosophila and discuss the potential impact future screens have in advancing our knowledge of the underlying genetic and neural circuits governing aggression.

Density‐dependent changes of mating system and family structure in Brandt's voles (Lasiopodomys brandtii)

AbstractA mating system is an important life history for animals dealing with changing environments. Population density affects the plasticity of a mating system and subsequently the family structure of animals, but its impacts on mating systems and social structures are rarely investigated by using molecular markers in field conditions. In this study, using microsatellite genetic markers, we examined the changes in the social and genetic mating system and family structure of Brandt's voles in the grassland of Inner Mongolia, China, under low‐, medium‐, and high‐density enclosures (each enclosure 0.48‐ha with 4 replicates.) We found, with the increase in population density of the founder voles introduced into the enclosure in early spring, both sexes increased their number of genetic mating partners, while males increased their social partners, resulting in a more promiscuous mating system. The number of genetic fathers and mothers per family, the number of social offspring per founder male or female, and the proportion of extra‐group offspring increased with increased density, indicating that the response of the family structure to density change, which is consistent with the change in mating system observed in our study. Both male and female voles had multiple mates, but males had a larger number of social and genetic mates, the number of social and genetic offspring, and the number of social and genetic families. Our study highlights the significance of density‐dependent plasticity of a mating system and family structure in affecting the population change of small mammals.

Ontogeny can provide insight into the roles of natural and sexual selection in cricket cuticular hydrocarbon evolution.

The often complex cocktails of hydrocarbon compounds found on the cuticles of insects can serve both naturally and sexually selected functions, contributing to an individual's ability to withstand water loss and attract mating partners. However, whether natural and sexual selection act synergistically or antagonistically on a species' cuticular hydrocarbon (CHC) profile remains unclear. Here, we examined the ontogeny of the CHC profile in a species of cricket, Teleogryllus oceanicus, while manipulating humidity during development. We predicted that juvenile crickets should produce only those compounds that contribute to desiccation resistance, while those compounds contributing specifically to male attractiveness should be produced only at sexual maturity. Further, if attractive CHCs come at a cost to desiccation resistance as predicted by some models of sexual selection, then males reared under low humidity should be constrained to invest less in attractive CHCs. Crickets reared under low humidity produced more long-chain methyl-branched alkanes, alkenes and alkadienes than did crickets reared under high humidity. The abundance of n-alkanes was unaffected by humidity treatment. Sexual dimorphism in the CHC profile was not apparent until adult emergence and became exaggerated 10 days after emergence, when crickets were sexually mature. Males produced more of the same compounds that were increased in both sexes under low humidity, but the humidity treatment did not interact with sex in determining CHC abundance. The data suggest that CHC profiles which protect crickets from desiccation might have synergistic effects on male attractiveness, as there was no evidence to suggest males trade-off a CHC profile produced in response to low humidity for one associated with sexual signalling.