Mango is traditionally classified as a climacteric fruit, in which ethylene and abscisic acid (ABA) play central roles in ripening. Mango fruit is cultivated only in tropical and some subtropical regions, therefore, to meet global demand, cold storage is widely used during transport, followed by a ripening period. This study evaluated the effects of simulated commercial transit cold storage (9 °C, > 85% relative humidity [RH]) used during the sea freight supply chain from the Southern hemisphere to the UK, by comparing the two main mango fruit cultivars (‘Keitt’ and ‘Kent’) imported into the UK market. Fruit were ripened with and without prior cold storage (control) and the synthesis and catabolism of key ripening regulators (ABA and its metabolites) as well as associated changes in quality attributes (internal colour, firmness, individual sugar content) were investigated. Cold storage delayed firmness loss, extending the postharvest life from 10 to 12 days to 29 days. ABA accumulated throughout ripening in control fruit but was depleted in cold-stored fruit for both cultivars, indicating its central role in mango ripening; cold storage fundamentally altered ABA dynamics by suppressing accumulation and modifying spatial distribution and conjugation patterns in a cultivar and pulp dependent manner. Additionally, sugar metabolism was altered, likely affecting taste, and meaning that, in cold-stored fruit, sugar content no longer correlated with total soluble solids (TSS). These findings highlight ABA as a potential target for postharvest management and underscore the need for integrated quality indices beyond TSS to improve fruit evaluation and reduce losses in global supply chains. • Cold storage depletes abscisic acid and disrupts normal ripening progression. • Sugar metabolism is altered, decoupling sugars from soluble solids content. • No chilling injury symptoms were observed after storage at 9 °C.

n-butanol treatment alleviates chilling injury of mango fruit by regulating membrane lipid metabolism

Efficacy of plant-based extracts on shelf life and postharvest quality of stored mango fruits was carried out. The experiment was laid out in a CRD with a 2 x 3 factorial experiment replicated 3 times. Healthy mango fruits of two varieties (Julie and Mabrouka) were harvested at harvest maturity and treated by immersing into three treatments Aloe vera gel, Aloe vera bark and a control. The fruits allowed to stay until the fruits were completely coated with the extracts, the stored at ambient temperature. Data obtained from the study was subjected to ANOVA, means were separated using (F-LSD) at 5% level of probability. The study results showed that plant-based extracts had significantly higher (≤0.05) effects on Weight Change, Total Soluble Solids (TSS) (°Brix), Firmness (N/cm), Lycopene (mg/100g), pH, Titratable Acidity (TA) (%), Decay Percentage (%) and Shelf-Life of stored mango fruits as compared to the control. There was no significant difference (≥0.05) in the interaction effect of variety and plant-based on postharvest weight change of fruits at 1 and 3WAS but significant effect was recorded on 0, 2 and 4WAS. TSS of fruits steadily increased in storage, however, the main effect of plant-based extracts as well as the interaction effect of variety and plant-based extracts on the TSS of mango fruits during storage was not significant only on OWAS. At the end of the storage Julie treated with Aloe vera gel (24.70) (°Brix), recorded the highest TSS. At the end of the storage, decay percentage of the untreated fruits was (18.78%), while fruits treated with Aloe vera extract (5.00%) and bark of Aloe vera (15.00%). These botanicals are environmentally friendly, cost effective, easy to produce and safe for consumers. Bio-based extracts of Aloe vera gel and Bark are recommended in the storage of flesh mango fruits.

Mango fruits undergo various biochemical changes and ripen rapidly after harvest. This study evaluated the effects of salicylic acid (SA) and hot water treatment (HWT) on the maintenance of postharvest quality of mango cv. Dashehari under ambient storage conditions. Physiologically mature fruits were subjected to different treatments, including SA 1 mM, SA 2 mM, HWT, HWT + SA 1 mM, HWT + SA 2 mM, and distilled water (control). Compared with untreated fruits, the combined treatments HWT + SA 1 mM and HWT + SA 2 mM significantly reduced weight loss, decay incidence, electrolyte leakage, reactive oxygen species accumulation, and activities of fruit softening enzymes, while better maintaining fruit firmness, soluble solids content, titratable acidity, and ascorbic acid during storage. The responses of these two combined treatments were statistically comparable for most quality attributes; however, fruits treated with HWT + SA 1 mM exhibited significantly lower malondialdehyde content after storage. These findings suggest that the integration of SA with HWT is an effective strategy for delaying ripening and preserving the postharvest biochemical quality of mango fruits under ambient conditions.

Fungal decay is a major limitation to the shelf-life and marketability of fresh mango. However, limited information is available regarding the development of multifunctional bioactive biomaterials capable of modulating fruit physiological and biochemical responses during storage. In this study, a sodium alginate edible coating enriched with plant-derived bioactives, including freeze-dried banana rachis leachate (Musa spp.) and an ethanolic leaf extract of Eugenia uniflora L., was evalueted as a biofunctional polymeric matrix designed to act as a bioactive interface between the fruit surface and the storage environment, to suppress postharvest decay and maintain quality in "Manila" mango during shelf-life storage. SEM and FTIR analyses confirmed the formation of a cross-linked coating matrix with heterogeneous structural features and suggested molecular interactions between alginate and phenolic constituents of the extracts. Fruits were stored for 16 days at 25°C and 50%-70% RH under four treatments: an uncoated control (T4) and three coating formulations (T1: extract + CaCl2, T2: extract only, T3: base coating). The best formulation (T1) reduced the rot severity by 62% relative to the control (p < 0.01), maintained higher firmness (+42%, p < 0.05), and delayed ripening as indicated by lower reducing sugars. T1 also enhanced retention of vitamin C (+32%), total phenolics (+27%), and antioxidant activity (+39%) compared to the control (p < 0.05), suggesting improved oxidative stability and delayed senescence processes. Importantly, T1 exhibited higher cumulative weight loss than the uncoated control, indicating that its performance was primarily associated with bioactive modulation rather than effective water-vapor barrier performance under in vivo conditions. These results demonstrate the potential of bioactive edible coatings derived from agro-industrial residues as sustainable biomaterial systems for postharvest biological preservation and functional quality retention in fresh produce. PRACTICAL APPLICATIONS: Natural edible coating made from alginate and plant-derived extracts may help reduce postharvest decay and maintain mango quality during storage. The formulation uses agro-industrial residues, offering a sustainable alternative to synthetic fungicides. This approach could support fruit producers and exporters seeking eco-friendly strategies to extend shelf-life and reduce postharvest losses in mango fruits.

Exploring mango (Mangifera indica) fruit peel extract mediated bio-preparation of CuO nanoparticles for biological, dye degradation and sensor applications

Pest infestation affects global agriculture, causing massive crop yield losses, ecosystem degradation, and negative economic impacts. High accuracy and efficiency in detecting small insect pests are crucial to avoid pesticide overuse and biodiversity loss. The objective of this research was to evaluate state-of-the-art object detection models (YOLOv7-v13) for multi-pest identification, focusing on small insect (< 5% image area) in mango (fruit flies), maize (fall armyworm), and cotton crops (pink bollworm) for effective decision-making. We developed the Five-Pest dataset comprising 17,251 images captured by IoT-based smart traps and 194,050 pest instances of five economically significant species (three fruit fly species, fall armyworm, and pink bollworm). Images were annotated via Roboflow, then augmented and smoothed ([Formula: see text]). YOLO models were trained under identical hyperparameters (40 epochs, batch 8, IoU = 0.8, max_det = 550). Performance was assessed by mAP@50, precision, recall, F1-score, and ROC-AUC. YOLOv9 achieved the highest mAP@50 (0.929), an average that includes lower precision such as fruit flies, underscoring the model's robustness across varied pest types. YOLOv9 was followed by YOLOv8 (0.924), YOLOv12 (0.922), YOLOv10 (0.921), YOLOv11 (0.909), and YOLOv13 (0.909), while YOLOv7 achieved mAP@50 of 0.899 for the Five-Pest dataset. YOLOv12 demonstrated comparable performance to YOLOv8 and YOLOv10 with stable precision and recall, whereas YOLOv13 achieved competitive detection performance but required substantially higher training time, indicating the accuracy-computational cost trade-off in later YOLO generations. All models detected fall armyworm with very high accuracy (mAP 0.96-0.99), while fruit flies (especially B. zonata) remained comparatively more challenging. Pink bollworm recognition remained consistently strong across all variants (mAP 0.91-0.97). To improve per-class detection robustness, an ensemble model averaging YOLOv7-YOLOv13 predictions showing enhanced consistency but requiring more computation. Here we identified the strengths and limitations of YOLO variants for small-insect detection, guiding the selection of models to help reducing pesticide use and enhancing environmental protection in precision agriculture.

Mango (Mangifera indica L.) represents one of the most significant fruit crops cultivated across multiple regions of Mexico. In recent years, cases of stem-end rot and dieback have been observed in mango-producing areas. This research aimed to characterize the diversity of Lasiodiplodia species associated with these symptoms, determine their geographic distribution in five Mexican states, and evaluate their pathogenicity on mango fruits. During 2014, samples exhibiting dieback and stem-end rot symptoms were collected from 27 commercial orchards located in five states, resulting in the obtention of 87 Lasiodiplodia isolates. From these, 36 representative isolates were selected and identified through phylogenetic analyses (ITS, tef1-α, tub2), employing the Maximum Likelihood and Bayesian Inference approach. Eight Lasiodiplodia species were resolved: L. brasiliense, L. laeliocattleyae, L. subglobosa, L. theobromae, L. iraniensis, L. mexicanensis, L. hyalina, and L. pseudotheobromae. Among them, L. brasiliense, L. laeliocattleyae, L. subglobosa, L. iraniensis, L. mexicanensis, and L. hyalina are reported for the first time in association with mango tissues in Mexico. Pathogenicity tests conducted on detached mango fruits using the mycelial plug inoculation method demonstrated that all species were capable of inducing necrotic lesions. However, L. laeliocattleyae and L. brasiliense exhibited the highest levels of aggressiveness, while L. mexicanensis, L. hyalina, and L. pseudotheobromae were the least aggressive.

Anthracnose, caused by Colletotrichum sp. isolate XCC1, is a major postharvest disease causing significant quality deterioration and economic losses in ‘Cat Chu’ mango during storage. This study evaluated the effectiveness of sodium alginate–carboxymethyl cellulose (SA-CMC) coating with natamycin for controlling anthracnose and maintaining postharvest fruit quality. Mango fruits were treated with the SA-CMC-Natamycin coating and stored under controlled conditions (25 ± 2 °C; RH = 60 ± 5%) to assess disease development, plant defense enzyme activities, and fruit quality attributes. Natamycin inhibited spore germination of Colletotrichum sp. isolate XCC1 with a Minimal Inhibitory Concentration (MIC) of 6.25 µg mL−1. The SA-CMC-Natamycin coating significantly reduced anthracnose development, resulting in a three-fold decrease in disease incidence and a 3.86-fold reduction in disease severity compared with the control on day 9 of storage. However, the persistence of the treatment was limited since no significant disease incidence reduction was observed after 15 days. The treatment also enhanced chitinase (CHI) and β-1,3-glucanase (GLU) activities and increased phenolic compound accumulation. In addition, the coating delayed fruit ripening by maintaining firmness, titratable acidity (TA), vitamin C, and chlorophyll while suppressing increases in color change and total soluble solids (TSS). These results demonstrate that SA-CMC-Natamycin coating is a promising eco-friendly strategy for controlling anthracnose and preserving postharvest quality of ‘Cat Chu’ mango.

Edible coatings play a crucial part in preventing fruit decay and extending their shelf life. However, a significant volume of water will collect on the fruit's surface due to respiratory metabolism during storage, causing the covering to disintegrate. Therefore, water-resistant coating is synthesized using hydroxypropyl methylcellulose, chitosan, and the hydrophobic nature of beeswax to preserve mango fruit. In this way, three types of edible coatings that consisted of hydroxypropyl methylcellulose, chitosan, and beeswax (HCB) were made by mixing the polyphenol extracts of orange peels (OPEs), apple peels (APEs), and spinach leaves (SPEs), respectively, which were named HCB-OPE, HCB-APE, and HCB-SPE. The prepared edible coatings were found to be stable and compatible, as determined by a Zetasizer, zeta potential, Fourier transform infrared (FTIR), atomic force microscopy, and antimicrobial analysis. Furthermore, the quality parameters of the applied coatings showed promising results on mango fruit during a 13-day storage period. The HCB-OPE coating was the most active at a dose of 50mg against Escherichia coli, with inhibition zones of 32.50±7.83, and HCB-APE with 50mg inhibited against Staphylococcus aureus by 29.56±5.64mm, respectively. The HCB-OPE coating showed a low weight loss of 1.20%±0.02% to 6.80%±0.06%. The coated mango fruits with HCB-OPE emulsion showed the lowest respiration rate (25.2±0.6mg CO2/kg/h).

A three-dimensional-simulation-assistant optimization of heat transfer and the influence of hot water treatment on postharvest quality, volatile flavor compounds and cuticular wax metabolisms of three cultivar mangoes.

Black flesh (BF) is an internal disorder in mango, characterized by the development of dark brown to black pigmentation in the inner mesocarp tissue during storage or transport. This study investigated the effects of harvest maturity, low storage or low transport temperatures, and 1-methylcyclopropene (1-MCP) on mango fruit susceptibility to BF. In the first experiment, 'Tommy Atkins', 'Kent', and 'Palmer' mangoes were harvested at two maturity stages and BF incidence was assessed after 30 days' storage at 8, 10, or 12.5 °C plus 7 days of shelf life at 20 °C. In the second experiment, 'Tommy Atkins' and 'Keitt' mangoes were also treated at harvest with the ethylene action inhibitor 1-MCP (0-800 nL L-1) and stored at 9 °C for 45 days, followed by 7 days of shelf life at 20 °C. The most effective 1-MCP treatment concentration (200 nL L-1) was then validated on mangoes for reduction of BF incidence and severity. The incidence of BF increased with early harvest and lower storage temperatures (≤10 °C), but it was not observed at 12.5 °C. 1-Methylcyclopropene at 200 nL L-1 markedly reduced the incidence and severity of BF, while suppressing respiration and ethylene production. This concentration also maintained flesh firmness and antioxidant capacity, performing in a similar way to higher concentrations. Early harvest and low storage temperature increased the incidence of BF whereas 1-MCP treatment reduced it. © 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

This study aimed to explain how key root-zone microorganisms affect mango growth and fruit quality in different regions. It provided theoretical support for targeted regional microbial control in mango cultivation. We chose three main mango-growing areas: Hainan, Yunnan and Southeast Asia. We used field test data from local mango orchards and high-throughput sequencing technology to analyze root-zone microbial community features. These features are shaped by soil and climate factors such as pH, altitude and humidity. The results showed a clear "core-stable + region-specific" distribution pattern in mango root-zone microbes. Stable core microbes meet mangoes' basic growth and metabolic needs, while area-specific microbes help the crop adapt better to local environmental stresses. Using too many chemical fertilizers and pesticides, together with extreme weather, breaks the balance of microbial communities. It causes soil quality to drop, reduces helpful microbial types, and then lowers mango yield and fruit quality. Targeted microbial control methods are effective. These methods include using custom microbial inoculants, breeding weather-resistant microbial strains and using integrated disease control. They restore soil microbial balance, improve soil health, and greatly increase mango yield, fruit quality and environmental stress resistance. This builds a solid scientific base for sustainable and region-specific mango growing.

Mango (Mangifera indica L.), recognized as the national fruit of Pakistan, stands as a vital tropical fruit crop notable for its economic value and significance in export markets. However, the cultivation of mango is severely hindered by infestations from fruit fly pests, particularly the Peach fruit fly, Bactrocera zonata (Hendel) (Diptera: Tephritidae). A comprehensive field study conducted at the Mango Research Institute (MRI) in Multan in 2025 aimed to assess the efficacy of various insecticides, botanical solutions, and bioagents using two specific techniques: Bait Application Technique (BAT) and Male Annihilation Technique (MAT) for effective fruit fly management. Findings from this study indicated that four treatments emerged as the most effective for each method based on metrics like percentage mortality and LT50 values. Within the scope of BAT, the combination of 10% jaggery with spinosad at a concentration of 0.02% proved to be the best field treatment. Conversely, in the context of MAT, a mixture of Ammonium acetate, lure, and spinosad in a 6:4:0.2 volume ratios demonstrated superior effectiveness. Spinosad, in particular, is recommended as an environmentally friendly alternative to the traditional organophosphate insecticide, malathion, due to its biodegradability, reduced toxicity to mammals, and overall safety for the environment. This research offers pivotal insights for the timely monitoring and implementation of Integrated Pest Management (IPM) strategies. These strategies notably include both the Male Annihilation Technique (MAT) and Bait Application Technique (BAT), alongside eco-friendly control measures. Understanding the pest’s population patterns and environmental drivers is essential for reducing fruit loss, minimizing pesticide use, and ensuring sustainable mango production in the region.

Abstract Global food loss and plastic pollution have emerged as critical environmental challenges, with over one-third of food produced for human consumption wasted annually and millions of tons of petroleum-based plastic packaging discarded in landfills and oceans. To address these issues, edible films and coatings derived from agricultural by-products have gained increasing attention as sustainable, biodegradable alternatives to conventional packaging. These films form semi-permeable barriers that regulate gas exchange, moisture transfer, and oxidation while offering the potential to incorporate natural antimicrobials and antioxidants. This review explores the valorisation of agricultural residues – including orange, lime, mango, pomegranate, potato, prickly pear, and other fruit peels – as renewable raw materials for the production of functional edible films. It highlights the historical evolution of edible packaging, the physicochemical properties of polysaccharide-, protein-, lipid-, and composite-based films, and modern processing techniques such as electro-spraying, solution casting, and 3D printing. Additionally, it emphasises advances in barrier performance, mechanical strength, and bioactivity resulting from the incorporation of plant extracts and nanomaterials. By converting agri-waste into value-added biodegradable films, this approach not only enhances food shelf life and safety but also contributes to circular economy goals and reduces environmental burden.

Starch degradation is a fundamental process underlying the development of key postharvest quality attributes in ripening mango fruit; however, its regulatory mechanisms remain incompletely understood. In this study, ethephon (ETH) and 1-methylcyclopropene (1-MCP) were used as ripening modulators to investigate the patterns of starch degradation and its transcriptional regulation in postharvest mango fruit. The results demonstrated that ETH fumigation accelerated the ripening process, while 1-MCP treatment effectively inhibited this process. Transcriptome and RT-qPCR analyses revealed and validated that six starch-degrading genes (MiAMY1.1, MiAMY3, MiBAM3, MiBAM9, MiGWD and MiPWD) were regulated in response to ETH. Furthermore, the transcription factor MiRAP2.11 was identified as a nucleus-localized transcriptional activator of MiBAM3 and MiPWD. Transient transformation assays demonstrated that MiRAP2.11 positively regulated starch degradation, thereby promoting development of ripening and quality attributes. The results indicate that MiRAP2.11 functions as a crucial regulator mediating ethylene-induced starch degradation during mango ripening.

Mango is one of the most abundant and economically valued fruits of Nepal and is widely treated with organophosphate and carbamate insecticides for quality and pest control. But in its non-scientific use and bulk applications, toxic residues are created that affect human health as well as the environment. Mango fruits used for this investigation were bought from four major bazaars Haatbazar, Purano Sabjimandi, Chatakpur Haatbazar, and Bhat-Bhateni and analyzed for quality through RBPR process, a pesticide residue screening system technology derived from AChE enzyme inhibition. The main objective of this study is to find the existence of pesticide residues in mango fruits sold in local markets of Dhangadhi Sub-Metropolitan City, Kailali, through the Rapid Bioassay of Pesticide Residue (RBPR) method. RBPR method was used because it is easy, cheap, and convenient to use, hence particularly ideal for developing countries like Nepal where access to advanced laboratory equipment might not be readily available. Spectrophotometric detection showed variation in residues with the highest inhibition at Chatakpur Haatbazar (17.514% carbamate, 12.123% organophosphate) and lowest at Bhat-Bhateni. All were below the 35% inhibition national safety limit and hence safe for consumption. Variability in residues on markets reflects variability in post-harvest and pesticide treatment. It requires vigilant monitoring, mass consciousness, training for farmers, and adoption of safer agricultural technologies like Integrated Pest Management (IPM). The study concludes that RBPR is an affordable, cost-saving, and practical approach for local food safety assessment and must be implemented at the national level policy for making Nepalese fruit production exportable and sustainable.

Mango fruits harvested at the right maturity ripen quickly but have a short shelf life, are prone to moisture loss, and are susceptible to microbial spoilage, resulting in severe post-harvest losses. This experiment was undertaken with the major objectives of increasing shelf life while maintaining quality. The study focuses on the green synthesis of silver nanoparticles (AgNPs) incorporated with different biopolymers (beeswax, arabic gum, chitosan, and guar gum), which formed the treatments, using the dip-coating method in two mango varieties, namely Alphonso and Bangalora. The AgNPs were green-synthesised using Sauropus androgynus (Chekkurmanis) leaf extracts and further characterised using Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) to confirm the presence of the nanomaterial. The coated fruits were stored at ambient room temperature, and their physiological and biochemical attributes were systematically monitored throughout the storage period which extended up to 16 days. The results revealed that postharvest treatments with beeswax (20% v/v) incorporated with AgNPS showed the most promising effects, extending shelf life to 16.72 days (Alphonso) and 18.86 days (Bangalora), compared to 12.8 days in uncoated fruits. This treatment also significantly reduced physiological weight loss (12%), maintained higher firmness (up to 14.43 N), and delayed increases in total soluble solids, sugars, and carotenoids. This research highlights the potential use of S. androgynus mediated AgNP-based biopolymer coatings as a sustainable postharvest method to prolong the shelf life of mangoes, making this the first report on mangoes with retention of fruit quality under ambient storage.

Females of the oriental fruit fly (Bactrocera dorsalis) disproportionately oviposit in unripe fruits, despite their lower nutritional value compared to ripe fruits, but the sensory cues driving such counterintuitive site selection have not been determined. Here, using an oriental fruit fly-mango interaction system, we identified a specific flavonoid compound, hesperidin, as a major avoidance cue driving oviposition site selection in this species. Hesperidin accumulates as mango fruits ripen, and high concentrations suppress both larval growth and adult emergence. This indicates that higher levels of hesperidin exert a direct harmful effect on B. dorsalis larvae within ripe fruits. Interestingly, we found that B. dorsalis females perceive hesperidin via gustatory sensors on the ovipositor. The BdorGr28b gene is strongly expressed in the ovipositor, and we showed that the corresponding protein is the major hesperidin receptor. These results demonstrate that gustatory sensing via the ovipositor drives maternal avoidance of hesperidin to benefit the offspring in B. dorsalis. Our study provides insight into oviposition site selection in this species and the broader functional repertoire of insect gustation.

Stem-End Rot (SER) is a postharvest disease of mango fruit causing heavy losses in storage. On August 16, 2025, 12 apparently healthy mango fruits (cv. SanNian) at 70‑80% maturity stage were harvested from an orchard in Xishuangbanna (21°08′N 101°50') and placed at room temperature (25-28℃). After 5-7 d, 8 fruits showed typical SER symptoms. Originally, small brown-black spots appeared around the peduncle of fruits, and then quickly expanded and merged. After sterilization with 75 % C2H6O for 3 min and 3% NaClO for 30 s, rinsing with sterile water and air-drying, fruit tissue pieces around 0.5 cm × 0.5 cm were aseptically cut from the lesion margins. Tissues pieces were placed on PDA plates and cultured in darkness at 28°C for 7 d (Tovar-Pedraza et al. 2020). Three isolates with similar morphology (BNSN-1, BNSN-2, and BNSN-3) were obtained via the single-spore isolation method, and the most virulent strain, BNSN-3, was used for further analysis after preliminary pathogenicity assays. Colonies covered the whole plate and appeared distinctive zonation appearance with irregular edges after 7 d incubation at 28 °C in the dark. The front of the colony was off-white to light grayish-white, while the reverse side was dark brown to grayish-brown with lighter periphery. Within 15 to 30 d, pycnidia appeared, growing in clusters, with a white-gray color and covered with velvety hyphae on the surface. Alpha conidia, 5.54~7.81×1.64~2.96 µm (n=30), were aseptate, transparent, glabrous, guttulate, fusiform to elliptical, tapering toward both ends, straight with obtuse apexes . Beta conidia, 19.05~29.41×1.37~1.98 µm (n=30) and less numeous than alpha conidia, were hyaline, smooth, straight, bent or hamular. These characteristics corresponded to the original descriptions of Diaporthe garethjonesii (Kytövuori 2016). The ITS (the internal transcribed spacer region), tef1-α (translation elongation factor 1-alpha), CAL (calmodulin), HIS (histone), and β-TUB (beta-tubulin) gene sequences of BNSN-3 were amplified, sequenced and submitted to GenBank (ITS: PX781187, tef1-α: PX789849, CAL: PX789851, HIS: PX789850, β-TUB: PX789852). BNSN-3 clustered with the type strain D. garethjonesii MFLUCC 120542aT (ITS: KT459423, tef1-α: KT459457, CAL: KT459470, β-TUB: KT459441) using maximum likelihood analysis by MEGA 7.0.21 (Norphanphoun et al. 2022). Based on morphological and molecular identification, BNSN-3 was definitively identified as D. garethjonesii. Pathogenicity assays were conducted on 15 healthy mango fruits (cv. Golek) by inoculating mycelial plugs onto needle-punctured wounds at the peduncle and equatorial regions according to Feng et al. (2023). Control fruits were treated identically, except that sterile PDA plugs were used instead of mycelial plugs. Three replicate fruits were used per each treatment and the experiment was performed three times. Typical SER symptoms appeared on inoculated mango fruits 7 days post-inoculation (dpi) after incubation at 28 ℃ and 95% ± 3% relative humidity, whereas the controls remained asymptomatic. The isolates obtained from symptomatic artificially inoculated fruits were identified as D. garethjonesii through ITS, tef1-α, CAL, HIS, and β-TUB gene sequencing, thus fulfilling Koch's postulates. To date, there is no reported case of plant disease including SER, induced by D. garethjonesii. This study extends the known host range of D. garethjonesii and is conducive to developing targeted control measures for mango SER.