Background/Objectives: Cowpea is a major source of dietary protein and plays a key role in sustainable agriculture across sub-Saharan Africa (SSA), Asia, and Latin America. Research efforts have focused mainly on enhancing productivity through higher yield and resistance to biotic and abiotic stresses in cowpea. Understanding the genetic basis of yield and associated agronomic traits is crucial for improving crop productivity. This study aims to identify quantitative trait loci (QTL) associated with grain yield and related traits in cowpea under regular rainfed conditions. Methods: We developed a set of 316 F6:7 recombinant inbred lines (RILs) mapping populations derived from a cross between RP270 and CB27 using a single-seed descent breeding method. The RILs and their two parental lines were evaluated in the field for two years, 2022 and 2023, at the International Institute of Tropical Agriculture (IITA) in Ibadan, Nigeria. The cowpea mid-density genotyping panel consisting of 2602 quality DArTag single nucleotide polymorphisms (SNPs) was used to genotype the RIL population. Results: Seven major QTLs, each explaining ≥10% of phenotypic variance, were detected for 100-seed weight, number of days to flower, number of pods per plant, number of branches per plant, and number of peduncles per plant. Putative genes associated with yield and related traits were identified within significant flanking markers. Further efforts to validate these loci will help to better understand their roles in yield and associated traits in cowpea.
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