Introduction Magnetic Nanoparticle Hyperthermia constitutes one of the latest promising treatments for cancerous tumors. The basic principle of hyperthermia lies on the necrosis of cancer cells for temperatures above the threshold of 42 °C, while healthy tissue undergoes negligible damage. Purpose The objectives of the conducted research included the setup and validation of a numerical simulation based on Rosensweig’s analytical relationships, in order to calculate the volumetric power dissipation due to magnetic nanoparticles in the matrix fluid or tissue (SAR – Specific Absorption Rate or SLP – Specific Loss Power). Materials and methods To simulate the experimental setup, as well as the heating process a numerical model was developed using Comsol Multiphysics software. Comsol solvers use FEM (Finite Element Methods) for approximating partial differential equations. To validate the numerical simulation, water based ferrofluids containing superparamagnetic iron oxide nanoparticles (Fe 3 O 4 ) in different concentrations were prepared. The samples were exposed to an alternating magnetic field produced by a solenoid connected to an “Easy Heat” system. Optical fiber was immersed in the subject ferrofluid in order to measure its temperature. The acquired data were processed to obtain hyperthermia heating curves. The geometry, parameters and variables of the experiment were implemented to Comsol Multiphysics. Results The simulated magnetic field, was validated using analytical expressions. Small divergence was observed, since Comsol software uses partial differential equations to compute the field amplitude and gradient accurately. The results produced by the numerical simulation concerning the heating process, were in agreement with those obtained during the experimental procedure. Conclusion In this study, the numerical simulation of ferrofluid heating was validated. The successful analytical description of this in vitro application, encourages the development of advanced models, designed for simulating in vivo applications.