Macroporous Anion Exchange Resin Research Articles

LANXESS expands its range for PFAS removal from water

On October 31, 2023, LANXESS reports that with the new macroporous anion exchange resin Lewatit MonoPlus TP 109, the company is expanding its range of selective resins for the efficient removal of contaminants such as per- and polyfluoroalkyl substances (PFAS) from water. The resin was presented for the first time at the international trade show Aquatech, which will take place in Amsterdam, the Netherlands, from November 6 to 9, 2023.

Green oxidative esterification of furfural over nano-Au supported on highly basic anion exchange resin beads

Au nanoparticles with an average diameter of 3.7 nm supported on the macroporous anion exchange resin beads with cationic –CH2N+Me3 functional groups and OH−as counter anions were first prepared, for catalyzing the oxidative esterification of biomass-derived furfural in methanol using green O2 as an oxidant. The main product methyl furoate with a high yield of 98% was obtained at 130 °C for 2 h in the absence of soluble alkali additives. The catalyst beads possessed a particle size range of 560∼700 μm inherited from the shaped support, enabling the separation of catalyst and liquid reaction medium by a simple decantation method. Catalyst poisoning experiments demonstrated Au activated superoxide radical O2·− and the basic OH− stored in the anion exchange resin are the decisive active components for the oxidative esterification, and the main product methyl furoate with a yield of 20% and a high selectivity of 96% was obtained under N2 atmosphere revealed the reaction in this catalytic system followed an oxidative dehydrogenation route. In all catalytic cases except for the catalyst poisoning experiments and the cases of low catalyst dosages, the by-product acetal was almost completely suppressed, probably attributed to the presence of the alkaline OH− in the anion exchange resin of the catalyst.

Highly selective removal of Technetium-99 using imidazolium-based macroporous anion exchange resins

Technetium-99 (99Tc), as a β-emitting radionuclide, is one of the main components of nuclear waste. The fast environmental mobility of 99TcO4- makes it a great threat to the environment and human beings. Although some adsorption materials have been developed to remove 99TcO4- from the nuclear wastewater, the low selectivity and poor stability under acid and radiation conditions limit their actual application. In this work, an N-methylimidazolium modified macroporous anion exchange resin (PMiMCl) was developed for the highly selective removal of 99TcO4-. Batch adsorption experiments demonstrated that the PMiMCl resin exhibited high adsorption capacity (Re(VII), 677.21 mg g−1) in a wide pH range (3–7). The resin showed high stability after acid (5 mol L-1 HCl), alkali (5 mol L-1 NaOH) and radiation (1000 KGy 60Co γ) treatment and the remove ability was little influenced. More importantly, in the presence of high concentration of competing anions, the removal efficiency of 99TcO4- with PMiMCl resin can also reach to 97.64%, which was much higher than those treated by commercial resins (93.02% for ZGANR170 and 93.10% for IRN78), demonstrating its good selectivity. The density functional theory calculations revealed that the high selectivity was attributed to the strongest interaction of imidazole cation in PMiMCl resin with 99TcO4- anion compared with NO3−, SO42− and Cl− anions. These superior features coupled with low cost and feasible for large scale production and application make PMiMCl resin great potential for 99Tc removal in nuclear wastewater.

Isolation and Purification of Mustard Glucosinolates by Macroporous Anion-Exchange Resin: Process Optimization and Kinetics’ Modelling

Glucosinolates (GSL) (β-thioglucoside-N-hydroxy sulfates) are rich-sulfur secondary metabolites raising potential biofumigation interest due to their biological properties. Sinigrin and gluconapin are the main glucosinolates present in brown mustard seeds (Brassica juncea). These glucosinolates are very suitable for the development of phytosanitary products due to their fungicidal, bactericidal and insecticidal effects. In this work, the purification of sinigrin and gluconapin extracted from defatted mustard seeds was studied using macroporous anion exchange resins. A strongly and a weakly anionic resin were first tested according to the nature of their functional group and through their selectivity towards glucosinolates. Anion-exchange resin purification was first studied in static (batch) mode in order to determine the optimal operating conditions; it was then tested in a dynamic (continuous) mode (column) to validate the process. In static mode, the adsorption behavior and characteristics of both resins were compared. The results showed that the strongly basic resin PA312LOH ensures better adsorption of glucosinolates and that the experimental data fit well with the Freundlich isotherm. Moreover, analysis showed that PA312LOH resin was selective for glucosinolates purification towards the proteins. The desorption of glucosinolates was then investigated. Firstly, the operating conditions were optimized by studying the effects of salt concentration and the eluate-resin ratio. This preliminary optimization allowed recovering 72.9% of intact sinigrin and the juice purity was increased from 43.05% to 79.63%. Secondly, dynamic (continuous mode) experiments allowed the recovery of 64.5% of sinigrin and 28% of gluconapin by varying the eluent ionic strength and the flow rate. Resin was finally successfully regenerated using NaOH.

Renewable molybdate complexes encapsulated in anion exchange resin for selective and durable removal of phosphate

The existence of many anions in wastewater reduces the removal efficiency of phosphate by adsorbents under realistic conditions. Facing this challenge, the study reports on an insistent and stable composite adsorbent of molybdate complexes Fe-(MoOx) embedded in a macroporous anion exchange resin (D-201). [Fe(MoOx)]-D-201 shows 93.7% adsorption capacity (28.3 mg/g) for phosphate even when the molar concentration of coexisting ions is 5 times higher than phosphate. The capacity of adsorbent is maintained more than 84.2% after five regeneration cycles to remove phosphate in the wastewater containing coexisting ions. The ability of highly selective removal of phosphate is maintained during the regeneration cycles explained by the change of the binding of molybdate clusters with phosphate, which is due to the different structures of molybdate clusters depending on various pH. In general, this work puts forward a new idea for the development of phosphorus removal adsorbents for the treatment of wastewater containing coexisting ions.

Removal of Per- and Polyfluoroalkyl Substances (PFASs) in Aqueous Film-Forming Foam (AFFF) Using Ion-Exchange and Nonionic Resins.

Despite benefits to the firefighting industry, the release of per- and polyfluoroalkyl substances (PFASs) from aqueous film-forming foam (AFFF) into aquatic systems poses significant risks to human health and other organisms. While anion-exchange technologies have proven to be effective for removing perfluoroalkyl acids (PFAAs) from water, their effectiveness for removing the diverse PFAS structures discovered in AFFF remains unknown. Here, we report on the adsorption of 75 PFASs, including 63 polyfluorinated substances, in a diluted AFFF mixture using 14 commercially available ion-exchange (IX)/nonionic resins and granular activated carbon (GAC). Results showed that anion-exchange resins (AERs) exhibited significant adsorption of PFASs compared to cation-exchange resins (CERs), nonionic resins (NIRs), and GAC regardless of the PFAS's predicted charge. Isotherm data showed that macroporous AERs have a higher PFAS adsorption capacity compared to gel-type AERs. Cross-correlation comparison of PFAS/Cl- selectivity coefficients (Kex) for each PFAS-AER combination showed that the hydrophobicity of the AER functional group, and polymer matrix played a dominant role in determining resin affinity for PFASs. PFAS structural characteristics also significantly affected adsorption, with increasing chain length and a net negative charge increasing the extent of adsorption. Results from this study provide guidelines for the selection of resins to adsorb a wider range of PFASs and meaningful insights for the development of quantitative models for IX treatment of AFFF-impacted water.

Novel Synthesis Method, Characterization and Bioactivities of a Copper(Ⅱ)-Hesperetin Complex Using Ion Exchange Column

A copper(Ⅱ)-hesperetin complex formulated as [Cu(L)2(H2O)2]and#183;H2O (andquot;Landquot; represented the ligand hesperetin) was synthesized using a novel ion-exchanging resin column method, based on the integration of the separation of hesperetin on hesperetin-loaded resins and the reaction of hesperetin with Cu2+ using CuCl2 as an eluent and reacting reagent. The feasibility of this new method was testified firstly. Results indicated the D296 macroporous strong basic anion exchange resin could effectively adsorb hesperetin and the saturated adsorption capacity was 143.5 mg per mL resin. The favorable concentration of CuCl2 was 0.4 mol/L for the elution and the coordination process of hesperetin. The characterization of the copper(Ⅱ) complex was done by theoretical quantum chemical calculation, elemental and thermal analyses, UV-Vis absorption spectra, FTIR spectra and XRD analysis, results testified that the 4-carbonyl group and 5-hydroxyl group of hesperetin were involved in the coordination reaction. The comparison between hesperetin and its copper(Ⅱ) complex about their bioactivities like the inhibitory effect of α-amylase, antioxidant activities and solubility were evaluated. Results showed the bioactivities of the copper(Ⅱ) complex was better than that of hesperetin.

Novel Synthesis Method, Characterization and Bioactivities of a Copper(Ⅱ)-Hesperetin Complex Using Ion Exchange Column

A copper(Ⅱ)-hesperetin complex formulated as [Cu(L)2(H2O)2]and#183;H2O (andquot;Landquot; represented the ligand hesperetin) was synthesized using a novel ion-exchanging resin column method, based on the integration of the separation of hesperetin on hesperetin-loaded resins and the reaction of hesperetin with Cu2+ using CuCl2 as an eluent and reacting reagent. The feasibility of this new method was testified firstly. Results indicated the D296 macroporous strong basic anion exchange resin could effectively adsorb hesperetin and the saturated adsorption capacity was 143.5 mg per mL resin. The favorable concentration of CuCl2 was 0.4 mol/L for the elution and the coordination process of hesperetin. The characterization of the copper(Ⅱ) complex was done by theoretical quantum chemical calculation, elemental and thermal analyses, UV-Vis absorption spectra, FTIR spectra and XRD analysis, results testified that the 4-carbonyl group and 5-hydroxyl group of hesperetin were involved in the coordination reaction. The comparison between hesperetin and its copper(Ⅱ) complex about their bioactivities like the inhibitory effect of α-amylase, antioxidant activities and solubility were evaluated. Results showed the bioactivities of the copper(Ⅱ) complex was better than that of hesperetin.

Synthesis optimization and X-ray absorption spectroscopy investigation of polymeric anion exchanger supported binary Fe/Mn oxides nanoparticles for enhanced As(III) removal

A macroporous anion exchange resin containing high density of positively-charged quaternary ammonium functional groups and supporting binary Fe/Mn oxides nanoparticles (HA502P-Fe/Mn) was synthesized for simultaneous As(III)oxidation and As(V) adsorption within a single bead. Various preparation parameters were investigated and optimized to achieve the highest As(III) removal efficiency, including metal oxides type, supporting materials, preparation solvents, and number of loading cycles. The results from five imaging techniques (SEM-EDX, TEM, XRD, XANES, EXAFS) confirmed that amorphous ferric and manganese oxides nanoparticles were successfully dispersed throughout the matrix of the parent macroporous anion exchanger. The changes in oxidation state of the Mn oxides from +4 to +2 and + 2 to +4 were observed by XANES during As(III) removal and regeneration runs, respectively. The EXAFS was used to investigate the neighborhood atoms surrounding arsenic during As(III) removal by HA502P-Fe/Mn. The bond distance between AsFe and AsMn of HA502P-Fe/Mn are 3.35 and 2.94 Å, respectively, suggesting that As(III) was oxidized to As(V) and adsorbed on the surface of Fe(III) and Mn(IV) oxide nanoparticles by formation of inner-sphere complex. As(III) was oxidized by MnO2 nanoparticles under the absence of oxygen environment and only As(V) was observed after adsorption. The equilibrium As(III) sorption isotherms, effect of pH, competing ions, and NOM were also evaluated. Fixed-bed column experiments using NSF challenge water standard 53 (300 μg As(III)/L, pH 6.5) were carried out and found that the material can remove As(III) equivalent to 4300 bed volumes (BVs) compared with 2000 BVs using commercial arsenic selective material (Layne-RT). The exhausted material can be regenerated using 10 BVs of regeneration solution and 98% of adsorbed arsenic can be recovered.

Enhanced selective removal of arsenic(V) using a hybrid nanoscale zirconium molybdate embedded anion exchange resin.

Selective removal of trace arsenic is crucial for obtaining safe drinking water. Here, the selective adsorptive performance of arsenate (As(V)) on a hybrid ZMAE (nanoscale zirconium molybdate embedded a macroporous anion exchange resin) was examined. It was found that the As(V) adsorption efficiency of ZMAE was almost retained in the presence of competing ions (NO3- or SO42-) up an [SO42-]/[As] or [NO3-]/[As] ratio of 150/1, whereas that of bare AE (anion exchange resin) was negligible for [SO4]/[As] over 15/1. In addition, the As(V) maximum adsorption capacity of ZMAE was found to be 41.2 mg/g, which is in contrast with the negligible adsorption of bare AE under sulfate-rich condition. The enhanced arsenate selectivity of ZMAE can be attributed to the excellent selectivity of ZM NPs (zirconium molybdate nanoparticles), which contributed up to 45% of the adsorption capacity of ZMAE. The behavior of ZMAE towards arsenate was compared with that towards phosphate showing similar adsorption performances between them, which indicates the similar affinity of ZMAE towards arsenate and phosphate. Finally, ZMAE examined for fixed-bed column adsorption for As(V) removal from synthetic As(V) water was effective for up to 5100 BVs, treating As(V) from 0.1 mg/L to below 0.01 mg/L (meeting the WHO guidelines).

Selective removal of closely related clipped protein impurities using poly(ethylenimine)- grafted anion-exchange chromatography resin

Proteolytic degradation is a serious problem that complicates downstream processing during production of recombinant therapeutic proteins. It can lead to decreased product yield, diminished biological activity, and suboptimal product quality. Proteolytic degradation or protein truncation is observed in various expression hosts and is mostly attributed to the activity of proteases released by host cells. Since these clipped proteins can impact pharmacokinetics and immunogenicity in addition to potency, they need to be appropriately controlled to ensure consistency of product quality and patient safety. A chromatography step for the selective removal of clipped proteins from an intact protein was developed in this study. Poly(ethylenimine)-grafted anion- exchange resins (PolyQUAT and PolyPEI) were evaluated and compared to traditional macroporous anion-exchange and tentacled anion-exchange resins. Isocratic retention experiments were conducted to determine the retention factors (k′) and charge factors (Z) were determined through the classical stoichiometric displacement model. High selectivity in separation of closely related clipped proteins was obtained with the PolyQUAT resin. A robust design space was established for the PolyQUAT chromatography through Design-Of-Experiments (DoE) based process optimization. Results showed a product recovery of up to 63% with purity levels >99.0%. Approximately, one-log clearance of host cell protein and two-logs clearance of host cell DNA were also obtained. The newly developed PolyQUAT process was compared with an existing process and shown to be superior with respect to the number of process steps, process time, process yield, and product quality.

Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology

Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm2/s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.

A reactor designed for the ultrasonic stimulation of enzymatic esterification

A laboratory scale ultrasonic flow reactor capable of enhancing enzymatic reactions has been built and characterized using as a model reaction the enzymatic synthesis of isoamyl acetate using Lipozyme 435 immobilized on a macroporous anion exchange resin. The efficiency of the reactor was determined in relation to ultrasonic power density (measured by 4-nitrophenol dosimetry), position of ultrasonic horn and temperature. The results show that ultrasound can enhance the process efficiency and also reduce the reaction time.

Development of nanoscale zirconium molybdate embedded anion exchange resin for selective removal of phosphate

Development of a selective adsorbent with an enhanced removal efficiency for phosphate from wastewater is urgently needed. Here, a hybrid adsorbent of nanoscale zirconium molybdate embedded in a macroporous anion exchange resin (ZMAE) is proposed for the selective removal of phosphate. The ZMAE consists of a low agglomeration of zirconium molybdate nanoparticles (ZM NPs) dispersed within the structure of the anion exchange (AE) resin. As major results, the phosphate adsorption capacity of the ZMAE (26.1 mg-P/g) in the presence of excess sulfate (5 mM) is superior to that of the pristine AE resin (1.8 mg-P/g) although their phosphate uptake capacity was similar in the absence of sulfate and these results were supported by the high selectivity coefficient of the ZMAE toward phosphate over sulfate (SPO4/SO4) more than 100 times compared to the pristine AE resin. This superior selective performance of the ZMAE for phosphate in the presence of sulfate ions is well explained by the role of the ZM NPs that contributed to 69% of the phosphate capacity which is based on an observation that the phosphate adsorption capacity of the ZM NPs is not affected by the presence of sulfate. In addition, the behavior of the selective phosphate removal by the ZMAE was well demonstrated by not only in the batch mode experiment with simulated Mekong river water and representative wastewater effluent but also in a column test.

Simultaneous uptake of NOM and Microcystin-LR by anion exchange resins: Effect of inorganic ions and resin regeneration

This study investigated the efficiency of a strongly basic macroporous anion exchange resin for the co-removal of Microcystin-LR (MCLR) and natural organic matter (NOM) in waters affected by toxic algal blooms. Environmental factors influencing the uptake behavior included MCLR and resin concentrations, NOM and anionic species, specifically nitrate, sulphate and bicarbonate. A860 resin exhibited an excellent adsorption capacity of 3800 μg/g; more than 60% of the MCLR removal was achieved within 10 min with a resin dosage of 200 mg/L (∼1 mL/L). Further, kinetic studies revealed that the overall removal of MCLR is influenced by both external diffusion and intra-particle diffusion. Increasing NOM concentration resulted in a significant reduction of MCLR uptake, especially at lower resin dosages, where a competitive uptake between the charged NOM fractions and MCLR was observed due to limited active sites. In addition, MCLR uptake was significantly reduced in the presence of sulphate and nitrate in the water matrix. Moreover, performance of the resin proved to be stable from one regeneration cycle to another. Approximately 80% of MCLR and 50% of dissolved organic carbon (DOC) were recovered in the regenerated brine. Evidences of resin saturation and site reduction were also observed after 2000 bed volumes (BV) of operation. For all the investigated water matrices, a resin dosage of 1000 mg/L (∼4.5 mL/L) was sufficient to lower MCLR concentration from 100 μg/L to below the World Health Organization guideline of 1 μg/L, while simultaneously providing more than 80% NOM removal.

A novel process for nitrate reduction in water using bimetallic Pd-Cu catalysts supported on ion exchange resin

In this work, a Pd-Cu catalyst supported on a macroporous anion exchange resin was used in two novel processes to treat water contaminated with nitrates. The catalytic performance was evaluated in a continuous fixed bed reactor. The process (Reaction+Ion Exchange) – Regeneration, named RIE-R, has two steps: 1- reaction and ion exchange (RIE); 2- Regeneration (R). It was found that a hydrogen flow rate increase does not affect the nitrate removal efficiency during the RIE step, while the selectivity to nitrogen decreased. The presence of competing ions (sulphate, bicarbonate, chlorides) led to a decrease in the nitrate removal efficiency. In any case, the water obtained in the process meets the limits established by the World Health Organization. During the regeneration step, carried out by bubbling hydrogen to the catalytic bed, 100% of nitrates present in the resin at the end of the RIE cycle were eliminated.The other process, named IE-R, has a first step in which only ion exchange of nitrates occurs, and a second one during which the catalyst is regenerated. This process has worse performance compared to the RIE-R. Only 36.7% of the nitrates present in the resin at the beginning of the regeneration were eliminated.The catalyst was characterized by CO chemisorption, SEM, EPMA, TEM, and XRD. Pd migration towards the external surface occurs during the reaction, and also a decreased in dispersion takes place, leading to a small decrease in activity detected in consecutive reaction-regeneration cycles.A regeneration mechanism is presented.

Removal of nitrate and Cr(VI) from drinking water by a macroporous anion exchange resin

AbstractNitrate and hexavalent chromium (Cr(VI)) are potentially hazardous to human health and ecosystems. In this study, an anion exchange resin (PBO-8) was synthesized for the removal of nitrate and Cr(VI) from aqueous solution. The PBO-8 resin was characterized with Fourier transform infrared spectroscopy, scanning electron microscopy and BET surface area analyses. The results of batch adsorption experiments indicated that the maximum equilibrium uptake of nitrate and Cr(VI) in single solute solutions were 21.13 and 45.02 mg/g, respectively. The maximum quantity of PBO-8 resin for the adsorption of nitrate and Cr(VI) were, respectively, decreased to 14.17 and 27.03 mg/g in a nitrate and Cr(VI) binary solution. The increase in temperature and decrease of pH enhanced the capacity of the PBO-8 resin for Cr(VI) adsorption, but such effects were less significant for nitrate. The Gibbs free energy change, enthalpy change, and entropy change illustrated that the adsorption of aqueous nitrate and Cr(VI) were f...

Characterization and evaluation of a macroporous anion exchange resin for nitrate removal from drinking water

The traditional drinking water purification process is inefficient in removing nitrate significantly. Ion exchange may be the most practical method for the removal of nitrate from drinking water. In this study, an anion exchange resin (PBE-8) was synthesized for selective nitrate removal. The PBE-8 resin was characterized by Fourier transform infrared spectrometry, scanning electron microscopy, and BET surface area analyses. Batch adsorption experiments were carried out to evaluate the thermodynamic and kinetic behaviors of the PBE-8 resin for the adsorption of nitrate. The results indicated that the maximum equilibrium nitrate uptake of PBE-8 was 26.19 mg N/g, which was higher than that of Purolite A 520E (23.07 mg N/g), but lower than that of HZ-222 (33.57 mg N/g). PBE-8 and Purolite A 520E demonstrated better adsorption of nitrate than HZ-222 in the presence of competing anions, such as sulfate, chloride, and bicarbonate in aqueous solution. The Gibbs free energy change (−12.15 to −1.15 kJ/mol), enthalpy change (0.35–7.73 kJ/mol), and entropy change (0.005–0.066 kJ/mol K) indicated that the adsorption of aqueous nitrate by PBE-8 was feasible, endothermic, and spontaneous. Furthermore, the nitrate adsorption by the PBE-8 resin showed a better fit to the Langmuir isotherm and pseudo-second-order kinetic models. Intraparticle diffusion was the main rate-limiting step.

Formation of carbon nanosheets via simultaneous activation and catalytic carbonization of macroporous anion-exchange resin for supercapacitors application.

Two-dimensional mesoporous carbon nanosheets (CNSs) have been prepared via simultaneous activation and catalytic carbonization route using macroporous anion-exchange resin (AER) as carbon precursor and ZnCl2 and FeCl3 as activating agent and catalyst, respectively. The iron catalyst in the skeleton of the AER may lead to carburization to form a sheetlike structure during the carbonization process. The obtained CNSs have a large number of mesopores, a maximum specific surface area of 1764.9 m(2) g(-1), and large pore volume of 1.38 cm(3) g(-1). As an electrode material for supercapacitors application, the CNSs electrode possesses a large specific capacitance of 283 F g(-1) at 0.5 A g(-1) and excellent rate capability (64% retention ratio even at 50 A g(-1)) in 6 mol L(-1) KOH. Furthermore, CNSs symmetric supercapacitor exhibits specific energies of 17.2 W h kg(-1) at a power density of 224 W kg(-1) operated in the voltage range of 0-1.8 V in 0.5 mol L(-1) Na2SO4 aqueous electrolyte, and outstanding cyclability (retains about 96% initial capacitance after 5000 cycles).

Separation and Purification of Sinigrin and Gluconapin from Defatted Indian Mustard Seed Meals by Macroporous Anion Exchange Resin and Medium Pressure Liquid Chromatography

Defatted seed meals, the byproducts of Indian mustard seed oil industry, which are just used as animal feedstock and nitrogen fertilizers, possess various nutrients and phytochemical compounds, such as sinigrin and gluconapin. The present study demonstrated a novel, low-cost, and efficient method for the co-production of sinigrin and gluconapin from defatted Indian mustard seed meals by D261 strong basic anion-exchange macroporous resin and medium pressure liquid chromatography (MPLC). The adsorption/desorption capacities of eight adsorbents characterized by BET, IR, and EDS were screened. 26.4 g defatted Indian mustard seed meals, which contained about 1210.3 mg sinigrin and 696.6 mg gluconapin, could produce 2059.6 mg of glucosinolate-rich extract of 53.67% sinigrin and 31.30% gluconapin after separation by D261 resin. Then, 949.40 mg of 97% sinigrin and 568.40 mg of 95% gluconapin could be obtained after the extracts were purified by MPLC, with the recovery of 76.03% of sinigrin and 77.38% of gluconapin. The products were assessed by analytical HPLC and characterized by UV, MS, 1H NMR, and 13C NMR. In conclusion, this method is practical and environmentally friendly, and it is a low-cost process to make full use of Indian mustard seeds.